WO1997024594A3 - Method of preparing and activating samples for radioimmunoassay and other test - Google Patents

Method of preparing and activating samples for radioimmunoassay and other test Download PDF

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Publication number
WO1997024594A3
WO1997024594A3 PCT/US1996/020724 US9620724W WO9724594A3 WO 1997024594 A3 WO1997024594 A3 WO 1997024594A3 US 9620724 W US9620724 W US 9620724W WO 9724594 A3 WO9724594 A3 WO 9724594A3
Authority
WO
WIPO (PCT)
Prior art keywords
radioimmunoassay
activating
sample
samples
preparing
Prior art date
Application number
PCT/US1996/020724
Other languages
French (fr)
Other versions
WO1997024594A2 (en
WO1997024594B1 (en
Inventor
Jose A Fernandez-Pol
Original Assignee
Jose A Fernandez-Pol
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jose A Fernandez-Pol filed Critical Jose A Fernandez-Pol
Priority to AU16885/97A priority Critical patent/AU1688597A/en
Publication of WO1997024594A2 publication Critical patent/WO1997024594A2/en
Publication of WO1997024594A3 publication Critical patent/WO1997024594A3/en
Publication of WO1997024594B1 publication Critical patent/WO1997024594B1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25625—Dilution
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

A method of activating target proteins in a biologic sample for radioimmunoassay or other immunoassay by adding the sample to a diluent containing no calcium or magnesium, heating the mixture in a boiling water bath for a designated period of time, cooling the sample, centrifuging the mixture to isolate the activated protein containing supernatant, and then vortexing the supernatant to ensure homogeneity of the protein.
PCT/US1996/020724 1995-12-29 1996-12-20 Method of preparing and activating samples for radioimmunoassay and other test WO1997024594A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU16885/97A AU1688597A (en) 1995-12-29 1996-12-20 Method of preparing and activating samples for radioimmunoassay and other test

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/581,072 1995-12-29
US08/581,072 US5668016A (en) 1995-12-29 1995-12-29 Method of preparing and activating samples for radioimmunoassay and other test

Publications (3)

Publication Number Publication Date
WO1997024594A2 WO1997024594A2 (en) 1997-07-10
WO1997024594A3 true WO1997024594A3 (en) 1997-08-21
WO1997024594B1 WO1997024594B1 (en) 1997-10-16

Family

ID=24323778

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/020724 WO1997024594A2 (en) 1995-12-29 1996-12-20 Method of preparing and activating samples for radioimmunoassay and other test

Country Status (3)

Country Link
US (1) US5668016A (en)
AU (1) AU1688597A (en)
WO (1) WO1997024594A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001058900A1 (en) * 2000-02-09 2001-08-16 Hokuriku Seiyaku Co., Ltd. 1h-imidazopyridine derivatives
US6803379B2 (en) * 2002-06-04 2004-10-12 Jose A. Fernandez-Pol Pharmacological agents and methods of treatment that inactivate pathogenic prokaryotic and eukaryotic cells and viruses by attacking highly conserved domains in structural metalloprotein and metalloenzyme targets

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4999304A (en) * 1987-12-28 1991-03-12 Miles Inc. Dynamic braking centrifuge
US5243041A (en) * 1991-08-22 1993-09-07 Fernandez Pol Jose A DNA vector with isolated CDNA gene encoding metallopanstimulin
US5367063A (en) * 1990-12-06 1994-11-22 Bomalaski John S Human phospholipase activating protein and methods for diagnosis of rheumatoid arthritis
US5395753A (en) * 1993-02-19 1995-03-07 Theratech, Inc. Method for diagnosing rheumatoid arthritis
US5494803A (en) * 1991-11-19 1996-02-27 North Carolina State University Immunodiagnostic assay using liposomes carrying labels thereof on outer liposome surface
US5530100A (en) * 1990-05-07 1996-06-25 Rhone-Poulenc Rorer Pharmaceuticals Inc. Methods for purification of recombinantly produced proteins

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4999304A (en) * 1987-12-28 1991-03-12 Miles Inc. Dynamic braking centrifuge
US5530100A (en) * 1990-05-07 1996-06-25 Rhone-Poulenc Rorer Pharmaceuticals Inc. Methods for purification of recombinantly produced proteins
US5367063A (en) * 1990-12-06 1994-11-22 Bomalaski John S Human phospholipase activating protein and methods for diagnosis of rheumatoid arthritis
US5243041A (en) * 1991-08-22 1993-09-07 Fernandez Pol Jose A DNA vector with isolated CDNA gene encoding metallopanstimulin
US5494803A (en) * 1991-11-19 1996-02-27 North Carolina State University Immunodiagnostic assay using liposomes carrying labels thereof on outer liposome surface
US5395753A (en) * 1993-02-19 1995-03-07 Theratech, Inc. Method for diagnosing rheumatoid arthritis

Also Published As

Publication number Publication date
WO1997024594A2 (en) 1997-07-10
US5668016A (en) 1997-09-16
AU1688597A (en) 1997-07-28

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