WO1997015584A3 - Use of linalool synthase in genetic engineering of scent production - Google Patents

Use of linalool synthase in genetic engineering of scent production Download PDF

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Publication number
WO1997015584A3
WO1997015584A3 PCT/US1996/016482 US9616482W WO9715584A3 WO 1997015584 A3 WO1997015584 A3 WO 1997015584A3 US 9616482 W US9616482 W US 9616482W WO 9715584 A3 WO9715584 A3 WO 9715584A3
Authority
WO
WIPO (PCT)
Prior art keywords
polypeptide
linalool synthase
genetic engineering
disclosed
nucleic acid
Prior art date
Application number
PCT/US1996/016482
Other languages
French (fr)
Other versions
WO1997015584A2 (en
Inventor
Eran Pichersky
Original Assignee
Univ Michigan
Eran Pichersky
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Michigan, Eran Pichersky filed Critical Univ Michigan
Priority to CA 2234647 priority Critical patent/CA2234647A1/en
Priority to AU76632/96A priority patent/AU7663296A/en
Priority to EP96939462A priority patent/EP0858507A2/en
Publication of WO1997015584A2 publication Critical patent/WO1997015584A2/en
Publication of WO1997015584A3 publication Critical patent/WO1997015584A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A purified S-linalool synthase polypeptide from Clarkia breweri is disclosed as is the recombinant polypeptide and nucleic acid sequences encoding the polypeptide. Also disclosed are antibodies immunoreactive with the purified peptide and with recombinant versions of the polypeptide. Methods of using the nucleic acid sequences, as well as methods of enhancing the smell and the flavor of plants expressing the nucleic acid sequences are also disclosed.
PCT/US1996/016482 1995-10-12 1996-10-15 Use of linalool synthase in genetic engineering of scent production WO1997015584A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA 2234647 CA2234647A1 (en) 1995-10-12 1996-10-15 Use of linalool synthase in genetic engineering of scent production
AU76632/96A AU7663296A (en) 1995-10-12 1996-10-15 Use of linalool synthase in genetic engineering of scent production
EP96939462A EP0858507A2 (en) 1995-10-12 1996-10-15 Use of linalool synthase in genetic engineering of scent production

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US514695P 1995-10-12 1995-10-12
US60/005,146 1995-10-12

Publications (2)

Publication Number Publication Date
WO1997015584A2 WO1997015584A2 (en) 1997-05-01
WO1997015584A3 true WO1997015584A3 (en) 1997-09-25

Family

ID=21714417

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/016482 WO1997015584A2 (en) 1995-10-12 1996-10-15 Use of linalool synthase in genetic engineering of scent production

Country Status (3)

Country Link
EP (1) EP0858507A2 (en)
AU (1) AU7663296A (en)
WO (1) WO1997015584A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU7377298A (en) * 1997-05-08 1998-11-27 Regents Of The University Of Michigan, The Methods and compositions for use of (iso)eugenol methyltransferase
WO1999023226A1 (en) * 1997-10-30 1999-05-14 The Regents Of The University Of Michigan Methods and compositions for use of benzylalcohol acetyl transferase
AR022383A1 (en) 1998-09-18 2002-09-04 Univ Kentucky Res Found SYNTHESES
AU2687000A (en) * 1999-02-22 2000-09-14 Yissum Research Development Company Of The Hebrew University Of Jerusalem Transgenic plants and method for transforming carnations
US7129393B1 (en) 1999-02-22 2006-10-31 Yissum Research Development Company Of The Hebrew University Transgenic plants and method for transforming carnations
EP1130104A1 (en) * 2000-02-16 2001-09-05 Stichting Dienst Landbouwkundig Onderzoek Reduction of in planta degradation of recombinant plant products
EP1231273A1 (en) * 2001-02-12 2002-08-14 Plant Research International B.V. Terpene synthase/cyclase and olefin synthase and uses thereof
AU2002233614B2 (en) * 2001-03-22 2007-05-24 Scentgene Pollination Ltd. Method of enhancing entomophilous
ES2324508B1 (en) * 2006-12-27 2010-05-31 Consejo Sup. De Invest. Cientificas IMPROVEMENT OF THE AROMATIC CONTENT OF WINES AND OTHER ALCOHOLIC BEVERAGES THROUGH THE USE OF MICROORGANISMS THAT, DURING FERMENTATION, PRODUCE SYNTHETIC MONOTERPEN.
JP5956252B2 (en) * 2011-06-10 2016-07-27 サントリーホールディングス株式会社 Polynucleotides encoding linalool synthase and uses thereof
CN110452918B (en) * 2019-08-30 2021-03-09 福建农林大学 Molecular method for creating novel high-aroma petunia germplasm

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992006206A1 (en) * 1990-10-09 1992-04-16 Imperial Chemical Industries Plc Dna, dna constructs, cells and plants derived therefrom
WO1992016611A1 (en) * 1991-03-12 1992-10-01 Pernod-Ricard Method of alcoholic fermentation to obtain muscat type aromas
WO1993007257A2 (en) * 1991-10-04 1993-04-15 Smart Plants International, Inc. Tissue-specific and developmentally regulated transcriptional sequences and uses thereof
WO1995011913A1 (en) * 1993-10-28 1995-05-04 Washington State University Research Foundation DNA ENCODING LIMONENE SYNTHASE FROM $i(MENTHA SPICATA)

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992006206A1 (en) * 1990-10-09 1992-04-16 Imperial Chemical Industries Plc Dna, dna constructs, cells and plants derived therefrom
WO1992016611A1 (en) * 1991-03-12 1992-10-01 Pernod-Ricard Method of alcoholic fermentation to obtain muscat type aromas
WO1993007257A2 (en) * 1991-10-04 1993-04-15 Smart Plants International, Inc. Tissue-specific and developmentally regulated transcriptional sequences and uses thereof
WO1995011913A1 (en) * 1993-10-28 1995-05-04 Washington State University Research Foundation DNA ENCODING LIMONENE SYNTHASE FROM $i(MENTHA SPICATA)

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
ARCH. BIOCHEM. BIOPHYS. (1995), 316(2), 803-7 CODEN: ABBIA4;ISSN: 0003-9861 *
CHEMICAL ABSTRACTS, vol. 118, no. 23, 7 June 1993, Columbus, Ohio, US; abstract no. 230249, SPENCER, ANDREW ET AL: "In vitro biosynthesis of monoterpenes by Agrobacterium transformed shoot cultures of two Mentha species" XP002036392 *
CHEMICAL ABSTRACTS, vol. 122, no. 15, 10 April 1995, Columbus, Ohio, US; abstract no. 181648, PICHERSKY, ERAN ET AL: "Purification and characterization of S-linalool synthase, an enzyme involved in the production of floral scent in Clarkia breweri" XP002036391 *
COLBY, S.M., ET AL.: "4S-limonene synthase from the oil glands of spearmint (Mentha spicata)", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 268, no. 31, 1993, pages 23016 - 23024, XP002036389 *
DUDAREVA N ET AL: "Evolution of floral scent in Clarkia: Novel patterns of S-linalool synthase gene expression in the C. breweri flower.", PLANT CELL 8 (7). 1996. 1137-1148., July 1996 (1996-07-01), XP002036387 *
MCGARVEY, D.J., ET AL.: "Terpenoid metabolism", THE PLANT CELL, vol. 7, no. 7, July 1995 (1995-07-01), pages 1015 - 1026, XP002036386 *
MOL J N M ET AL: "FLORICULTURE: GENETIC ENGINEERING OF COMMERCIAL TRAITS", TRENDS IN BIOTECHNOLOGY, vol. 13, no. 9, September 1995 (1995-09-01), pages 350 - 355, XP002024912 *
PELLEGRINESCHI, A., ET AL.: "Improvement of ornamental characters and fragrance production in lemon-scented geranium through genetic transformation by Agrobacterium rhizogenes", BIOTECHNOLOGY, vol. 12, no. 1, January 1994 (1994-01-01), pages 64, XP002036390 *
PHYTOCHEMISTRY (1993), 32(4), 911-19 CODEN: PYTCAS;ISSN: 0031-9422 *
PICHERSKY E ET AL: "Floral Scent Production in Clarkia (Onagraceae): I. Localization and Developmental Modulation of Monoterpene Emission and Linalool Synthase Activity.", PLANT PHYSIOLOGY (ROCKVILLE) 106 (4). 1994. 1533-1540., XP002036388 *

Also Published As

Publication number Publication date
WO1997015584A2 (en) 1997-05-01
AU7663296A (en) 1997-05-15
EP0858507A2 (en) 1998-08-19

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