WO1996024876A1 - Chambre de comptage et son procede de fabrication - Google Patents

Chambre de comptage et son procede de fabrication Download PDF

Info

Publication number
WO1996024876A1
WO1996024876A1 PCT/NL1996/000061 NL9600061W WO9624876A1 WO 1996024876 A1 WO1996024876 A1 WO 1996024876A1 NL 9600061 W NL9600061 W NL 9600061W WO 9624876 A1 WO9624876 A1 WO 9624876A1
Authority
WO
WIPO (PCT)
Prior art keywords
adhesive
counting chamber
granules
plates
dimension
Prior art date
Application number
PCT/NL1996/000061
Other languages
English (en)
Inventor
Hendrik Jan Westendorp
Alfons Petrus Antonius Gerrit De Kock
Original Assignee
Hendrik Jan Westendorp
Kock Alfons Petrus Antonius Ge
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hendrik Jan Westendorp, Kock Alfons Petrus Antonius Ge filed Critical Hendrik Jan Westendorp
Priority to AU48466/96A priority Critical patent/AU4846696A/en
Priority to EP96904341A priority patent/EP0871914A1/fr
Publication of WO1996024876A1 publication Critical patent/WO1996024876A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/061—Counting droplets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0822—Slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces

Definitions

  • the invention relates to a counting chamber for determining the number of particles present in a fluid, said counting chamber substantially consisting of two parallel, at least partially transparent plates, which are bonded together by means of an adhesive and between which a practically entirely enclosed space is present, said space having a substantially constant dimension in the direction perpendicularly to said plates, which dimension will hereafter be called the cross dimension or the width of the space.
  • a counting chamber of this type is for example used for carrying out a semen analysis, whereby the sperm concentration of a sperm sample is determined.
  • the small spacing between the surfaces of the glass plates leads to a capillary action, so that a sample charged to the counting chamber will spread over the space of the counting chamber.
  • a very thin layer of the material of the sample is then formed, which can subsequently be observed optically, possibly by means of a microscope.
  • one of the glass plates is for example provided with a check pattern, it becomes possible to count the number of particles per unit area which are present in the sample.
  • the space of a counting chamber used for semen analysis has a cross dimension of for example 20 ⁇ m, whilst the space of a counting chamber used for analysing blood or other body fluids will have a cross dimension of for example 100 ⁇ m.
  • one of the plates may be locally provided with a coating having a predetermined thickness, after which the two plates are bonded together at the location of said coating.
  • the space of the counting chamber will be bounded by the coating that has been applied and will have a cross dimension which corresponds with the thickness of the coating.
  • the object of the invention is to provide a counting chamber which can be manufactured in a simple and reliable manner.
  • the counting chamber is provided with plates whose facing sides are substantially flat, and the cross dimension of the space is substantially equal to the thickness of the adhesive layer present between the plates.
  • the adhesive contains granules having dimensions which determine the spacing between the plates.
  • said granules are spherical and consist of glass or a similar material.
  • the presence of the granules prevents the adhesive layer from being flattened during the bonding process.
  • the adhesive contains granules having a predetermined maximum diameter of approximately 10 - 150 ⁇ m, preferably approximately 20 ⁇ 100 ⁇ m, as well as granules having a smaller diameter.
  • the thickness of the adhesive layer will substantially correspond with the dimension of the granules having the maximum diameter, whilst the granules having a smaller diameter are not a determining factor with regard to the cross dimension. In this manner a very accurate width dimension of the space, which is constant over the entire space, can be obtained in a reliable and simple manner.
  • a transparent part of at least one of said plates may be provided with a grid or a check pattern, for example consisting of squares.
  • the number of particles per volume unit of the fluid present in the counting chamber can be determined in a simple manner by counting the number of particles present within one square.
  • the invention furthermore relates to a method for manufacturing a counting chamber, wherein two at least partially transparent plates are bonded together in mutually parallel relationship, whereby the thickness of the adhesive layer is according to the invention substantially equal to the cross dimension of the space of the counting chamber.
  • the adhesive may thereby comprise granules having a dimension which determines the thickness of the adhesive layer.
  • the adhesive contains spheres having a predetermined maximum diameter of approximately 10 - 150 ⁇ m, as well as spheres having a smaller diameter, which spheres are selected by sifting spheres of various diameters.
  • a problem in the production of spheres, for example glass spheres, is to produce spheres having a predetermined average dimension (diameter) within a predetermined range of dimensions.
  • said spheres are sifted mechanically by means of a screen which is set to a diameter which approximately corresponds with the average diameter of the spheres, this results in an amount of spheres, a relatively large number of which corresponds with the diameter to which the screen is set.
  • the spheres selected by sifting may then be added to the adhesive with which the plates of the counting chamber are bonded together.
  • the granules are sifted prior to being added to the adhesive, by means of a screen having gaps of a predetermined width. Since gaps having a predetermined width are used, only those granules which are capable of being incorporated in an adhesive layer which corresponds with the width of the gaps can pass said gaps.
  • the sifting of the granules preferably takes place in a fluid, for example water, so that the granules can be transported over and/or through the screen in a simple manner.
  • the granules may be electrically charged in such manner as to cause mutual repulsion among said granules.
  • this may for example take place by rubbing the granules in a woollen cloth.
  • the lumping of the granules is prevented by providing the granules with a coating of a material whose cohesion is smaller than the adhesion between said adhesive and said material.
  • a mould containing a material having substantially the shape and dimension of the space of the counting chamber is placed on a flat side of one ot said plates, after which an adhesive is applied, which adhesive is skimmed off by means of an object having a substantially straight edge, which is moved over said mould.
  • an adhesive is applied to the places present between the material of the mould, in a thickness which is determined by the thickness of the material of the mould. This makes it possible to apply a properly distributed adhesive layer of a predetermined thickness in an accurate manner.
  • said thickness is slightly greater, for example 1 - 10%, than the cross dimension of the space of the counting chamber and the maximum dimension of- the granules present in the adhesive, so that it will be possible to exert sufficient force on the adhesive when pressing down the adhesive between the plates.
  • the mould consists of a plate-shaped element, which comprises unperforated material at the location where the space of the counting chamber is to be formed, and which is perforated in those places where the adhesive is to be applied.
  • a mould may be obtained by locally providing a layer consisting of a gauze or an apertured fabric with an impermeable material, which bonds in said layer, so that the adhesive, when being skimmed off, can only pass through the layer at those locations where no impermeable material is present.
  • a similar technique is used in screen-printing.
  • an adhesive is used which slightly shrinks during the curing process, so that the plates are slightly pulled down on the granules during said curing.
  • Figure 1 is a front view of a counting chamber formed by two bonded-together glass plates
  • Figure 2 is a plan view of the device according to Figure
  • Figure 3 is a sectional view along line III-III in Figure
  • Figure 4 is a sectional view of two bonded-together glass plates .
  • Figures 1, 2 and 3 shows two glass plates 1, 2, which are bonded together by means of an adhesive layer 3.
  • the adhesive layer 3 is not present on the entire surface of the glass plates 1, 2, but only in circumferential area 4, as is shown in Figure 1.
  • a space not containing any adhesive is present within circumferential area 4, so that a chamber 5 is formed between glass plates 1, 2.
  • Said chamber 5 communicates with the outside via an opening 6.
  • a counting chamber for single use has been formed, which may have a width of for example 10 - 20 ⁇ m between the substantially parallel glass plates.
  • a fluid is charged to the chamber 5, for example by means of a pipette, said fluid will spread over the entire chamber, at least over a large part thereof, as a result of the capillary action, so that a thin layer of fluid is formed, which has a predetermined thickness everywhere.
  • this fluid may be observed, for example by means of a microscope, in order to be able to detect particles which may be present in said fluid.
  • a semen analysis may be carried out in such a chamber.
  • the two glass plates may be entirely transparent, but it is also possible to provide a check pattern on one glass plate or on both, in order to make it easier to count the number of particles per unit area present in the fluid.
  • a suitable counting chamber 5 can only be formed when the adhesive layer 3 has a constant thickness over the entire surface on which the adhesive layer is present. Because the adhesive layer is very thin, for example between about 10 ⁇ m and 100 ⁇ m, and because a high degree of accuracy of the thickness of the adhesive layer is required, it is very difficult to manufacture the illustrated device by maintaining the required spacing between the glass plates by mechanical means during the curing of the adhesive.
  • Figure 4 diagrammatically shows on a slightly enlarged scale the manner in which the thickness of the adhesive layer is maintained at a predetermined level.
  • the adhesive layer 3 present between glass plates 1, 2 comprises spherical glass granules 7, 8, which have been selected such that the diameter of the largest granules 7 corresponds with the thickness of the desired thickness of the adhesive layer.
  • Smaller granules 8, which may also be present in the adhesive layer do not have an influence on the thickness of the adhesive layer. Since the granules have been selected by means of a mechanical screen, it is possible that said smaller granules 8 are contained in the adhesive layer, but the adhesive layer thickness is determined by the largest granules 7 that were able to pass said screen.
  • the adhesive used may be a silicone adhesive, preferably an adhesive is used which contains acrylic monomers and/or acrylic urethane oligomers.
  • An adhesive of this type is commercially available under the name "Viprocoll Adhesive UV2000” or "UV200 Stabifix Adhesive” from Visprox B.V., Haarlem ( ⁇ L) , for example.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Adhesives Or Adhesive Processes (AREA)

Abstract

Chambre de comptage destinée à compter les particules présentes dans un liquide et sensiblement constituée de deux plaques parallèles (1, 2) au moins partiellement transparentes et liées l'une à l'autre au moyen d'une colle (3), entre lesquelles est formé un volume (5) quasi fermé, de dimension sensiblement constante dans le sens perpendiculaire aux dites plaques. Les faces des plaques (1, 2) qui se situent en regard l'une de l'autre son sensiblement plates, et ladite dimension de l'espace est sensiblement égale à l'épaisseur de la couche de colle présente entre les plaques. La colle (3) renferme des billes de verre (7, 8) de diamètre maximal prédéterminé, lequel diamètre correspond à ladite dimension du volume. On peut obtenir les billes de verre par tamisage de billes de diamètres différents.
PCT/NL1996/000061 1995-02-07 1996-02-07 Chambre de comptage et son procede de fabrication WO1996024876A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU48466/96A AU4846696A (en) 1995-02-07 1996-02-07 Counting chamber and method for manufacturing a counting chamber
EP96904341A EP0871914A1 (fr) 1995-02-07 1996-02-07 Chambre de comptage et son procede de fabrication

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
NL9500222 1995-02-07
NL9500222 1995-02-07
NL1000607A NL1000607C1 (nl) 1995-02-07 1995-06-20 Telkamer en werkwijze voor het vervaardigen van een telkamer
NL1000607 1995-06-20

Publications (1)

Publication Number Publication Date
WO1996024876A1 true WO1996024876A1 (fr) 1996-08-15

Family

ID=26642105

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL1996/000061 WO1996024876A1 (fr) 1995-02-07 1996-02-07 Chambre de comptage et son procede de fabrication

Country Status (4)

Country Link
EP (1) EP0871914A1 (fr)
AU (1) AU4846696A (fr)
NL (1) NL1000607C1 (fr)
WO (1) WO1996024876A1 (fr)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008063124A1 (fr) * 2006-11-21 2008-05-29 Gyros Patent Ab Procédé de liaison d'un dispositif microfluidique et dispositif microfluidique
EP1949310A2 (fr) * 2005-10-19 2008-07-30 Wardlaw Partners, LP Appareil et procede permettant d'effectuer des decomptes dans un echantillon de fluide biologique
US8241572B2 (en) 2004-04-07 2012-08-14 Abbott Point Of Care, Inc. Disposable chamber for analyzing biologic fluids
US9199233B2 (en) 2010-03-31 2015-12-01 Abbott Point Of Care, Inc. Biologic fluid analysis cartridge with deflecting top panel
CN105728069A (zh) * 2016-01-30 2016-07-06 深圳市贝沃德克生物技术研究院有限公司 用于快速自检血液的多通道微流控芯片
US9579651B2 (en) 2009-12-18 2017-02-28 Abbott Point Of Care, Inc. Biologic fluid analysis cartridge
US9873118B2 (en) 2010-12-30 2018-01-23 Abbott Point Of Care, Inc. Biologic fluid analysis cartridge with sample handling portion and analysis chamber portion
US10782306B2 (en) 2015-12-24 2020-09-22 Koninklijke Philips N.V. Method and a system for determinations of cell suspensions

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3447863A (en) * 1966-07-11 1969-06-03 Sodell Research & Dev Co Method for preparing a slide for viewing
EP0030102A1 (fr) * 1979-11-23 1981-06-10 Bl Cars Limited Couches adhésives à épaisseur contrôlée
US4801411A (en) * 1986-06-05 1989-01-31 Southwest Research Institute Method and apparatus for producing monosize ceramic particles
EP0321889A2 (fr) * 1987-12-22 1989-06-28 Board Of Regents, The University Of Texas System Méthodes et appareils pour la détermination quantitative de constituants dans des échantillons liquides
EP0437408A2 (fr) * 1990-01-12 1991-07-17 United Medical Systems Israel Ltd. Dispositif à usage unique pour déterminer la qualité des spermatozoides
WO1991020009A1 (fr) * 1990-06-15 1991-12-26 Doody Michael C Procede et dispositif ameliores d'utilisation de microspheres en microscopie et de quantification de test postcoital

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3447863A (en) * 1966-07-11 1969-06-03 Sodell Research & Dev Co Method for preparing a slide for viewing
EP0030102A1 (fr) * 1979-11-23 1981-06-10 Bl Cars Limited Couches adhésives à épaisseur contrôlée
US4801411A (en) * 1986-06-05 1989-01-31 Southwest Research Institute Method and apparatus for producing monosize ceramic particles
EP0321889A2 (fr) * 1987-12-22 1989-06-28 Board Of Regents, The University Of Texas System Méthodes et appareils pour la détermination quantitative de constituants dans des échantillons liquides
EP0437408A2 (fr) * 1990-01-12 1991-07-17 United Medical Systems Israel Ltd. Dispositif à usage unique pour déterminer la qualité des spermatozoides
WO1991020009A1 (fr) * 1990-06-15 1991-12-26 Doody Michael C Procede et dispositif ameliores d'utilisation de microspheres en microscopie et de quantification de test postcoital

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8241572B2 (en) 2004-04-07 2012-08-14 Abbott Point Of Care, Inc. Disposable chamber for analyzing biologic fluids
US9084995B2 (en) 2004-04-07 2015-07-21 Abbott Laboratories Disposable chamber for analyzing biologic fluids
US10578602B2 (en) 2004-04-07 2020-03-03 Abbott Laboratories Disposable chamber for analyzing biologic fluids
US9696252B2 (en) 2005-10-19 2017-07-04 Abbott Laboratories Apparatus for performing counts within a biologic fluid sample
EP1949310A2 (fr) * 2005-10-19 2008-07-30 Wardlaw Partners, LP Appareil et procede permettant d'effectuer des decomptes dans un echantillon de fluide biologique
EP1949310A4 (fr) * 2005-10-19 2011-11-23 Wardlaw Partners Lp Appareil et procede permettant d'effectuer des decomptes dans un echantillon de fluide biologique
US8158434B2 (en) 2005-10-19 2012-04-17 Abbott Laboratories Method for performing counts within a biologic fluid sample
US20100044376A1 (en) * 2006-11-21 2010-02-25 Gyros Patent Ab Method of bonding a micrifluidic device and a microfluidic device
WO2008063124A1 (fr) * 2006-11-21 2008-05-29 Gyros Patent Ab Procédé de liaison d'un dispositif microfluidique et dispositif microfluidique
US9993817B2 (en) 2009-12-18 2018-06-12 Abbott Point Of Care, Inc. Biologic fluid analysis cartridge
US9579651B2 (en) 2009-12-18 2017-02-28 Abbott Point Of Care, Inc. Biologic fluid analysis cartridge
US9199233B2 (en) 2010-03-31 2015-12-01 Abbott Point Of Care, Inc. Biologic fluid analysis cartridge with deflecting top panel
US9873118B2 (en) 2010-12-30 2018-01-23 Abbott Point Of Care, Inc. Biologic fluid analysis cartridge with sample handling portion and analysis chamber portion
US10391487B2 (en) 2010-12-30 2019-08-27 Abbott Point Of Care, Inc. Biologic fluid analysis cartridge with sample handling portion and analysis chamber portion
US11583851B2 (en) 2010-12-30 2023-02-21 Abbott Point Of Care Inc. Biologic fluid analysis cartridge with sample handling portion and analysis chamber portion
US10782306B2 (en) 2015-12-24 2020-09-22 Koninklijke Philips N.V. Method and a system for determinations of cell suspensions
CN105728069A (zh) * 2016-01-30 2016-07-06 深圳市贝沃德克生物技术研究院有限公司 用于快速自检血液的多通道微流控芯片
CN105728069B (zh) * 2016-01-30 2021-01-19 深圳市安测健康信息技术有限公司 用于快速自检血液的多通道微流控芯片

Also Published As

Publication number Publication date
EP0871914A1 (fr) 1998-10-21
NL1000607C1 (nl) 1996-08-07
AU4846696A (en) 1996-08-27

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