WO1996021042A3 - Primers for the pcr amplification of metastatic sequences - Google Patents

Primers for the pcr amplification of metastatic sequences Download PDF

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Publication number
WO1996021042A3
WO1996021042A3 PCT/US1996/000461 US9600461W WO9621042A3 WO 1996021042 A3 WO1996021042 A3 WO 1996021042A3 US 9600461 W US9600461 W US 9600461W WO 9621042 A3 WO9621042 A3 WO 9621042A3
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WO
WIPO (PCT)
Prior art keywords
metastatic
sequences
primers
specific
pcr amplification
Prior art date
Application number
PCT/US1996/000461
Other languages
French (fr)
Other versions
WO1996021042B1 (en
WO1996021042A9 (en
WO1996021042A2 (en
Inventor
Robert A Edelstein
Robert B Moreland
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Univ Boston
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Boston filed Critical Univ Boston
Priority to AU48978/96A priority Critical patent/AU4897896A/en
Publication of WO1996021042A2 publication Critical patent/WO1996021042A2/en
Publication of WO1996021042A3 publication Critical patent/WO1996021042A3/en
Publication of WO1996021042B1 publication Critical patent/WO1996021042B1/en
Publication of WO1996021042A9 publication Critical patent/WO1996021042A9/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/112—Disease subtyping, staging or classification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to methods for the detection of metastatic diseases such as metastatic prostate, breast and lung carcinoma, in fresh or fixed biological samples. Nucleic acids are purified from fixed samples of patient tissue or fluid suspected to contain metastatic tissue and reverse transcribed to cDNA. Metastatic-specific sequences within the cDNA are amplified by polymerase chain reaction. Primers for PCR amplification comprise sequences from the exonic regions of a metastatic-specific expression product that span regions of expressed RNA that are not degraded even after long-term storage in paraffin. Metastatic-specific expression products include, for example, nucleic acids that contain sequences of the prostate specific antigen gene for use in the detection of metastatic prostate carcinoma. The resulting amplified nucleic acid sequences will include metastatic-specific sequences which can be easily detected. The invention also relates to nucleic acid primers which can be used for PCR amplification of metastatic-specific sequences such as sequences which correspond to the exonic regions of the PSA gene, and to kits which comprise these primers and other reagents useful for the rapid detection of metastatic diseases.
PCT/US1996/000461 1995-01-04 1996-01-04 Primers for the pcr amplification of metastatic sequences WO1996021042A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU48978/96A AU4897896A (en) 1995-01-04 1996-01-04 Primers for the pcr amplification of metastatic sequences

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36870695A 1995-01-04 1995-01-04
US08/368,706 1995-01-04

Publications (4)

Publication Number Publication Date
WO1996021042A2 WO1996021042A2 (en) 1996-07-11
WO1996021042A3 true WO1996021042A3 (en) 1996-09-12
WO1996021042B1 WO1996021042B1 (en) 1996-10-17
WO1996021042A9 WO1996021042A9 (en) 1996-11-28

Family

ID=23452406

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/000461 WO1996021042A2 (en) 1995-01-04 1996-01-04 Primers for the pcr amplification of metastatic sequences

Country Status (2)

Country Link
AU (1) AU4897896A (en)
WO (1) WO1996021042A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6103237A (en) * 1993-07-22 2000-08-15 Hybritech Incorporated Stable variant hK2 polypeptide
CA2189774A1 (en) * 1994-05-10 1995-11-16 Donald J. Tindall Recombinant hk2 polypeptide
US5919638A (en) * 1996-10-08 1999-07-06 Abbott Laboratories Reagents and methods useful for detecting prostate tumors
DE19782095T1 (en) * 1996-11-06 2000-03-23 Sequenom Inc DNA diagnosis based on mass spectrometry
EP0941368A2 (en) * 1996-11-14 1999-09-15 Mayo Foundation For Medical Education And Research Method for detection of metastatic prostate cancer
US6479263B1 (en) 1996-11-14 2002-11-12 Baylor College Of Medicine Method for detection of micrometastatic prostate cancer
US6235486B1 (en) 1997-06-20 2001-05-22 Mayo Foundation For Medical Education & Research Method for detection of breast cancer
WO1999037811A1 (en) * 1998-01-21 1999-07-29 Urocor, Inc. Biomarkers and targets for diagnosis, prognosis and management of prostate disease
US5972615A (en) 1998-01-21 1999-10-26 Urocor, Inc. Biomarkers and targets for diagnosis, prognosis and management of prostate disease
EP0999268B1 (en) 1998-10-07 2008-04-02 Boehringer Mannheim Gmbh New gene with upregulated expression in metastatic human tumor cells and a protein coded thereby, methods of production, and use thereof
US6551778B1 (en) 1999-01-28 2003-04-22 Gen-Probe Incorporated Nucleic acid sequences for detecting genetic markers for cancer in a biological sample
WO2002081656A2 (en) * 2001-04-05 2002-10-17 The Henry M. Jackson Foundation Enhanced diagnostic potential of prostate-specific antigen expressing cells
US7910295B2 (en) * 2002-11-14 2011-03-22 John Wayne Cancer Institute Detection of micro metastasis of melanoma and breast cancer in paraffin-embedded tumor draining lymph nodes by multimarker quantitative RT-PCR
CN113549689B (en) * 2021-09-23 2021-12-24 默禾医疗科技（上海）有限公司 Kit and method for detecting PROS1 gene exon

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991012343A2 (en) * 1990-02-07 1991-08-22 F. Hoffmann-La Roche Ag Detection of point mutations in genes encoding gtp binding proteins
EP0520794A1 (en) * 1991-06-26 1992-12-30 F. Hoffmann-La Roche Ag Methods for detection of carcinoma metastases by nucleic acid amplification
WO1994010343A1 (en) * 1992-10-29 1994-05-11 Thomas Jefferson University Methods of detecting micrometastasis of prostate cancer
WO1994027152A1 (en) * 1993-05-14 1994-11-24 Nordion International Inc. Detection of prostate-specific antigen in breast tumors

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991012343A2 (en) * 1990-02-07 1991-08-22 F. Hoffmann-La Roche Ag Detection of point mutations in genes encoding gtp binding proteins
EP0520794A1 (en) * 1991-06-26 1992-12-30 F. Hoffmann-La Roche Ag Methods for detection of carcinoma metastases by nucleic acid amplification
WO1994010343A1 (en) * 1992-10-29 1994-05-11 Thomas Jefferson University Methods of detecting micrometastasis of prostate cancer
WO1994027152A1 (en) * 1993-05-14 1994-11-24 Nordion International Inc. Detection of prostate-specific antigen in breast tumors

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
EDELSTEIN R ET AL: "A rapid and simple method for the detection of prostate-specific antigen mrna in archival tissue specimens using a reverse transcription-polymerase chain reaction assay", UROLOGY, vol. 45, no. 4, April 1995 (1995-04-01), pages 547 - 603, XP000575614 *
FINKE ET AL: "An improved strategy and a useful housekeeping gene for RNA analysis from formalin-fixed parraffin-embedded tissues by pcr", BIOTECHNIQUES, vol. 14, no. 3, March 1993 (1993-03-01), pages 448 - 453, XP002007865 *
MARTIN R ET AL: "Detection of ptc in archival formalin-fixed paraffin-embedded tissue", DIAGNOSTIC MOLECULAR PATHOLOGY, vol. 3, no. 4, December 1994 (1994-12-01), pages 233 - 9, XP000575617 *
MIES C: "A simple, rapid methoid for isolating RNA from paraffin-embedded tissues for reverse transcription-polymerase chain reaction (RT-PCR)", THE JOURNAL OF HISTOCHEMISTRY AND CYTOCHEMISTRY, vol. 42, no. 6, June 1994 (1994-06-01), pages 811 - 13, XP000575605 *

Also Published As

Publication number Publication date
AU4897896A (en) 1996-07-24
WO1996021042A2 (en) 1996-07-11

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