WO1996017940A3 - Method of desulfurization of fossil fuel with flavoprotein - Google Patents

Method of desulfurization of fossil fuel with flavoprotein Download PDF

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Publication number
WO1996017940A3
WO1996017940A3 PCT/US1995/015865 US9515865W WO9617940A3 WO 1996017940 A3 WO1996017940 A3 WO 1996017940A3 US 9515865 W US9515865 W US 9515865W WO 9617940 A3 WO9617940 A3 WO 9617940A3
Authority
WO
WIPO (PCT)
Prior art keywords
fossil fuel
flavoprotein
organic sulfur
biocatalyst
molecules
Prior art date
Application number
PCT/US1995/015865
Other languages
French (fr)
Other versions
WO1996017940A2 (en
Inventor
Charles H Squires
Wan Ji
Lei Xi
Beatrice C Ortego
Olga S Pogrebinsky
Kevin A Gray
John D Childs
Original Assignee
Energy Biosystems Corp
Charles H Squires
Wan Ji
Lei Xi
Beatrice C Ortego
Olga S Pogrebinsky
Kevin A Gray
John D Childs
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Energy Biosystems Corp, Charles H Squires, Wan Ji, Lei Xi, Beatrice C Ortego, Olga S Pogrebinsky, Kevin A Gray, John D Childs filed Critical Energy Biosystems Corp
Priority to EP95942572A priority Critical patent/EP0797668A2/en
Priority to JP8517746A priority patent/JPH10510423A/en
Priority to AU43755/96A priority patent/AU693633B2/en
Priority to BR9510082A priority patent/BR9510082A/en
Publication of WO1996017940A2 publication Critical patent/WO1996017940A2/en
Publication of WO1996017940A3 publication Critical patent/WO1996017940A3/en
Priority to MXPA/A/1997/004165A priority patent/MXPA97004165A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10G—CRACKING HYDROCARBON OILS; PRODUCTION OF LIQUID HYDROCARBON MIXTURES, e.g. BY DESTRUCTIVE HYDROGENATION, OLIGOMERISATION, POLYMERISATION; RECOVERY OF HYDROCARBON OILS FROM OIL-SHALE, OIL-SAND, OR GASES; REFINING MIXTURES MAINLY CONSISTING OF HYDROCARBONS; REFORMING OF NAPHTHA; MINERAL WAXES
    • C10G32/00—Refining of hydrocarbon oils by electric or magnetic means, by irradiation, or by using microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Production Of Liquid Hydrocarbon Mixture For Refining Petroleum (AREA)

Abstract

The invention relates to the discovery that the rate of reaction of the desulfurization of fossil fuels is enhanced by the addition of a flavoprotein to the biocatalyst. The invention is drawn to a method for enhancing the rate of desulfurizing a fossil fuel containing organic sulfur compounds, comprising the steps of: a) contacting the fossil fuel with an aqueous phase containing a biocatalyst capable of cleaving carbon-sulfur bonds and a rate-enhancing amount of a flavoprotein, thereby forming a fossil fuel and aqueous phase mixture; b) maintaining the mixture of step (a) under conditions sufficient for cleavage of the carbon-sulfur bonds of the organic sulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; and c) separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase. The invention also relates to a recombinant microorganism containing one or more recombinant DNA molecules which encode a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and which encode a flavoprotein. The invention also relates to a composition comprising (a) a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and (b) a flavoprotein.
PCT/US1995/015865 1994-12-08 1995-12-05 Method of desulfurization of fossil fuel with flavoprotein WO1996017940A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP95942572A EP0797668A2 (en) 1994-12-08 1995-12-05 Method of desulfurization of fossil fuel with flavoprotein
JP8517746A JPH10510423A (en) 1994-12-08 1995-12-05 Desulfurization method of fossil fuel using flavin protein
AU43755/96A AU693633B2 (en) 1994-12-08 1995-12-05 Method of desulfurization of fossil fuel with flavoprotein
BR9510082A BR9510082A (en) 1994-12-08 1995-12-05 Method to increase the rate of bio-sulfurization of a fossil fuel containing organic sulfur compounds, DNA molecule, microorganism and composition
MXPA/A/1997/004165A MXPA97004165A (en) 1994-12-08 1997-06-05 Method of desulfuration of combustible fosil conflavoprote

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35175494A 1994-12-08 1994-12-08
US08/351,754 1994-12-08

Publications (2)

Publication Number Publication Date
WO1996017940A2 WO1996017940A2 (en) 1996-06-13
WO1996017940A3 true WO1996017940A3 (en) 1996-08-29

Family

ID=23382228

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1995/015865 WO1996017940A2 (en) 1994-12-08 1995-12-05 Method of desulfurization of fossil fuel with flavoprotein

Country Status (8)

Country Link
US (2) US5733773A (en)
EP (1) EP0797668A2 (en)
JP (1) JPH10510423A (en)
CN (1) CN1169159A (en)
AU (1) AU693633B2 (en)
BR (1) BR9510082A (en)
CA (1) CA2206987A1 (en)
WO (1) WO1996017940A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10510423A (en) * 1994-12-08 1998-10-13 エナジー バイオシステムズ コーポレーション Desulfurization method of fossil fuel using flavin protein
US5804433A (en) * 1996-10-23 1998-09-08 Energy Biosystems Corporation Rhodococcus flavin reductase complementing DszA and DszC activity
US5952208A (en) * 1997-04-07 1999-09-14 Energy Biosystems Corporation Dsz gene expression in pseudomonas hosts
AU2505700A (en) * 1999-01-14 2000-08-01 Energy Biosystems Corporation Growth of biocatalyst within biodesulfurization system
FR2795807B1 (en) 1999-07-02 2001-07-27 Coflexip DEVICE FOR LIMITING THE PROPAGATION OF A DEFORMATION IN A DOUBLE WALL TUBE
US6402939B1 (en) 2000-09-28 2002-06-11 Sulphco, Inc. Oxidative desulfurization of fossil fuels with ultrasound
EP1957118B1 (en) * 2005-10-28 2013-06-19 Indian Oil Corporation Limited Method for bio-oxidative desulfurization of liquid hydrocarbon fuels and product thereof
CN105087060A (en) * 2015-09-21 2015-11-25 广西大学 Method for applying insulin and cholic acid to coker diesel oil desulfurization
CN105219431B (en) * 2015-09-21 2017-02-01 广西大学 Desulphurization method for coking diesel oil by using cholic acid
CN105112096B (en) * 2015-09-21 2017-02-01 广西大学 Method of applying insulin to coking diesel oil desulfurization

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0445896A2 (en) * 1990-02-28 1991-09-11 Institute Of Gas Technology Bacterial produced extracts and enzymes for cleavage of organic C-S bonds
WO1994001563A1 (en) * 1992-07-10 1994-01-20 Energy Biosystems Corporation Recombinant dna encoding a desulfurization biocatalyst

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2641564A (en) * 1948-03-31 1953-06-09 Texaco Development Corp Process of removing sulfur from petroleum hydrocarbons and apparatus
US4562156A (en) * 1983-07-11 1985-12-31 Atlantic Research Corporation Mutant microorganism and its use in removing organic sulfur compounds
US5104801A (en) * 1990-01-05 1992-04-14 Institute Of Gas Technology Mutant microorganisms useful for cleavage of organic c-s bonds
US5002888A (en) * 1990-01-05 1991-03-26 Institute Of Gas Technology Mutant microorganisms useful for cleavage of organic C-S bonds
US5198341A (en) * 1990-01-05 1993-03-30 Institute Of Gas Technology Useful for cleavage of organic C-S bonds Bacillus sphaericus microorganism
US5358870A (en) * 1990-02-28 1994-10-25 Institute Of Gas Technology Microemulsion process for direct biocatalytic desulfurization of organosulfur molecules
US5344778A (en) * 1990-02-28 1994-09-06 Institute Of Gas Technology Process for enzymatic cleavage of C-S bonds and process for reducing the sulfur content of sulfur-containing organic carbonaceous material
US5356813A (en) * 1992-04-30 1994-10-18 Energy Biosystems Corporation Process for the desulfurization and the desalting of a fossil fuel
JPH10510423A (en) * 1994-12-08 1998-10-13 エナジー バイオシステムズ コーポレーション Desulfurization method of fossil fuel using flavin protein

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0445896A2 (en) * 1990-02-28 1991-09-11 Institute Of Gas Technology Bacterial produced extracts and enzymes for cleavage of organic C-S bonds
WO1994001563A1 (en) * 1992-07-10 1994-01-20 Energy Biosystems Corporation Recombinant dna encoding a desulfurization biocatalyst

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
S.A. DENOME ET AL: "Characterization of the desulfurization genes from Rhodococcus sp. strain IGTS8", JOURNAL OF BACTERIOLOGY, vol. 176, no. 21, November 1994 (1994-11-01), pages 6707 - 6716, XP000567805 *
TAKASHI OHSHIRO ET AL: "Enzymatic desulfurization of dibenzothiophene by a cell-free system of Rhodococcus erythropolis D-1", FEMS MICROBIOLOGY LETTERS, vol. 118, 1994, pages 341 - 344, XP002002324 *
TAKASHI OHSHIRO ET AL: "Involvement of flavin coenzyme in dibenzothiophene degrading enzyme system from Rhodococcus erythropolis D-1", BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY, vol. 59, no. 7, July 1995 (1995-07-01), pages 1349 - 1351, XP002002325 *

Also Published As

Publication number Publication date
WO1996017940A2 (en) 1996-06-13
AU693633B2 (en) 1998-07-02
JPH10510423A (en) 1998-10-13
MX9704165A (en) 1997-09-30
US5733773A (en) 1998-03-31
AU4375596A (en) 1996-06-26
EP0797668A2 (en) 1997-10-01
BR9510082A (en) 1998-07-14
CN1169159A (en) 1997-12-31
US5985650A (en) 1999-11-16
CA2206987A1 (en) 1996-06-13

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