WO1996015266A3 - Eukaryotic cell assay using ribosomal rna detection - Google Patents

Eukaryotic cell assay using ribosomal rna detection Download PDF

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Publication number
WO1996015266A3
WO1996015266A3 PCT/US1995/014579 US9514579W WO9615266A3 WO 1996015266 A3 WO1996015266 A3 WO 1996015266A3 US 9514579 W US9514579 W US 9514579W WO 9615266 A3 WO9615266 A3 WO 9615266A3
Authority
WO
WIPO (PCT)
Prior art keywords
ribosomal rna
assay
eukaryotic cell
effect
cells
Prior art date
Application number
PCT/US1995/014579
Other languages
French (fr)
Other versions
WO1996015266A2 (en
Inventor
Janice T Brown
Yu Ping Maguire
Original Assignee
Dade Int Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dade Int Inc filed Critical Dade Int Inc
Priority to AU42331/96A priority Critical patent/AU4233196A/en
Publication of WO1996015266A2 publication Critical patent/WO1996015266A2/en
Publication of WO1996015266A3 publication Critical patent/WO1996015266A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/136—Screening for pharmacological compounds

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

An assay to determine the effect of an agent on eukaryotic cell viability or cell proliferation is disclosed. The assay involves quantitating the amount of ribosomal RNA in cells by hybridizing the ribosomal RNA in the sample with a detection probe. The amount of rRNA present in the sample is correlated with the effect of the agent on the viability or proliferation of the cells. The assay may be used to test the effect of chemotherapeutic agents or growth supplements on eukaryotic cells, or to measure histocompatibility in a mixed lymphocyte response assay.
PCT/US1995/014579 1994-11-09 1995-11-09 Eukaryotic cell assay using ribosomal rna detection WO1996015266A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU42331/96A AU4233196A (en) 1994-11-09 1995-11-09 Eukaryotic cell assay using ribosomal rna detection

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33673094A 1994-11-09 1994-11-09
US08/336,730 1994-11-09

Publications (2)

Publication Number Publication Date
WO1996015266A2 WO1996015266A2 (en) 1996-05-23
WO1996015266A3 true WO1996015266A3 (en) 1996-09-12

Family

ID=23317405

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1995/014579 WO1996015266A2 (en) 1994-11-09 1995-11-09 Eukaryotic cell assay using ribosomal rna detection

Country Status (2)

Country Link
AU (1) AU4233196A (en)
WO (1) WO1996015266A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994002636A1 (en) * 1992-07-28 1994-02-03 Hitachi Chemical Company Ltd. Gene detection system

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994002636A1 (en) * 1992-07-28 1994-02-03 Hitachi Chemical Company Ltd. Gene detection system

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
FERRARI, SERGIO ET AL: "Abundance of the primary transcript and its processed product of growth-related genes in normal and leukemic cells during proliferation and differentiation", CANCER RES. (1992), 52(1), 11-16 CODEN: CNREA8;ISSN: 0008-5472, XP002001931 *
GREENHALGH DA ET AL: "Effects of 5-fluorouracil on cytotoxicity and RNA metabolism in human colonic carcinoma cells.", CANCER CHEMOTHER PHARMACOL, 1989, 25 (1) P37-44, GERMANY, WEST, XP002001929 *
GRUMMT, INGRID: "Specific transcription of mouse ribosomal DNA in a cell-free system that mimics control in vivo", PROC. NATL. ACAD. SCI. U. S. A. (1981), 78(2), 727-31 CODEN: PNASA6;ISSN: 0027-8424, XP002001932 *
PAJOR, L. ET AL: "Flow cytometric measurement of rRNA levels detected by fluorescent in situ hybridization in differentiating K-562 cells", HISTOCHEMISTRY (1991), 96(1), 73-81 CODEN: HCMYAL;ISSN: 0301-5564, XP002001930 *
WALLNER, GUENTER ET AL: "Optimizing fluorescent in situ hybridization with rRNA targeted oligonucleotide probes for flow cytometric identification of microorganisms", CYTOMETRY (1993), 14(2), 136-43 CODEN: CYTODQ;ISSN: 0196-4763, XP002001933 *

Also Published As

Publication number Publication date
AU4233196A (en) 1996-06-06
WO1996015266A2 (en) 1996-05-23

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