WO1995032297B1 - Novel chromosome fragment and its use as a vector - Google Patents
Novel chromosome fragment and its use as a vectorInfo
- Publication number
- WO1995032297B1 WO1995032297B1 PCT/GB1995/001195 GB9501195W WO9532297B1 WO 1995032297 B1 WO1995032297 B1 WO 1995032297B1 GB 9501195 W GB9501195 W GB 9501195W WO 9532297 B1 WO9532297 B1 WO 9532297B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- chromosome
- vector according
- fragment
- human
- vector
- Prior art date
Links
- 210000000349 Chromosomes Anatomy 0.000 title claims abstract 7
- 210000001182 Chromosomes, Human, Y Anatomy 0.000 claims abstract 5
- 238000000034 method Methods 0.000 claims abstract 3
- 210000002230 Centromere Anatomy 0.000 claims abstract 2
- 230000022131 cell cycle Effects 0.000 claims abstract 2
- 229920003013 deoxyribonucleic acid Polymers 0.000 claims abstract 2
- 238000001502 gel electrophoresis Methods 0.000 claims abstract 2
- 238000005204 segregation Methods 0.000 claims abstract 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 230000000717 retained Effects 0.000 claims 1
- 210000004507 Chromosomes, Artificial Anatomy 0.000 abstract 1
- 238000006062 fragmentation reaction Methods 0.000 abstract 1
- 238000001415 gene therapy Methods 0.000 abstract 1
- 230000002068 genetic Effects 0.000 abstract 1
Abstract
The present invention relates to the field of molecular genetics and in particular to artificial chromosomes, to their preparation in particular using telomere-directed chromosome fragmentation techniques and to their use as DNA vectors, for instance for application in gene therapy and animal transgenesis. Vectors of the invention comprise a chromosome fragment which fragment is at least partly responsible for centromere function of the parent chromosome and is capable of replication and segregation during cell cycle, and which is of such a size that it can be resolved using gel electrophoresis. Suitable fragments are derived from the human Y chromosome.
Claims
1. A vector comprising a chromosome fragment other than a yeast chromosome fragment, which fragment comprises sequences which have centromeric activity, is capable of replication and segregation during cell cycle, and is of such a size that it can be resolved using gel electrophoresis.
2. A vector according to claim 1 which is derived from the human Y chromosome.
3. A vector according to claim 2 comprising a linear derivative of the human Y chromosome which includes at least some alphoid DNA.
4. A vector according to claim 2 or claim 3 which is derived solely from the short arm of the human Y chromosome.
5. A vector according to claims 2 or claim 3 which is derived solely from the long arm of the human Y chromosome.
6. A vector according to any one of the preceding claims which is less than 6Mb in size.
7. A vector according to claim 6 which is less than 3.5 megabase in size.
8. A process for obtaining a vector according to any one of the preceding claims which process comprises dissecting a chromosome in such a manner that a fragment retaining centromere function is retained.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU25343/95A AU2534395A (en) | 1994-05-25 | 1995-05-25 | Novel chromosome fragment and its use as a vector |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9410446.0 | 1994-05-25 | ||
| GB9410446A GB9410446D0 (en) | 1994-05-25 | 1994-05-25 | Novel chromosome fragment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1995032297A1 WO1995032297A1 (en) | 1995-11-30 |
| WO1995032297B1 true WO1995032297B1 (en) | 1996-01-18 |
Family
ID=10755674
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/GB1995/001195 WO1995032297A1 (en) | 1994-05-25 | 1995-05-25 | Novel chromosome fragment and its use as a vector |
Country Status (4)
| Country | Link |
|---|---|
| AU (1) | AU2534395A (en) |
| GB (1) | GB9410446D0 (en) |
| WO (1) | WO1995032297A1 (en) |
| ZA (1) | ZA954300B (en) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2042093C (en) * | 1990-05-09 | 2002-12-24 | Gyula Hadlaczky | Cell line carrying an excess of mammalian centromeres |
| US5869294A (en) * | 1995-06-07 | 1999-02-09 | Case Western Reserve University | Method for stably cloning large repeating DNA sequences |
| US6348353B1 (en) * | 1995-06-07 | 2002-02-19 | Case Western Reserve University | Artificial mammalian chromosome |
| US6632976B1 (en) | 1995-08-29 | 2003-10-14 | Kirin Beer Kabushiki Kaisha | Chimeric mice that are produced by microcell mediated chromosome transfer and that retain a human antibody gene |
| US6025155A (en) * | 1996-04-10 | 2000-02-15 | Chromos Molecular Systems, Inc. | Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes |
| US6077697A (en) * | 1996-04-10 | 2000-06-20 | Chromos Molecular Systems, Inc. | Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes |
| CA2263823C (en) | 1996-08-26 | 2010-11-23 | Tsuneko Okazaki | Mammalian artificial chromosomes |
| TWI255853B (en) | 1998-08-21 | 2006-06-01 | Kirin Brewery | Method for modifying chromosomes |
| WO2002010375A1 (en) * | 2000-07-17 | 2002-02-07 | Jiahui Xia | Human source gene leading sequence, gene vector and gene expression strategy |
| US7041870B2 (en) | 2000-11-30 | 2006-05-09 | Medarex, Inc. | Transgenic transchromosomal rodents for making human antibodies |
| CN100448992C (en) | 2001-05-11 | 2009-01-07 | 麒麟医药株式会社 | Artificial human chromosome containing human antibody lambda light chain gene |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4889806A (en) * | 1987-04-15 | 1989-12-26 | Washington University | Large DNA cloning system based on yeast artificial chromosomes |
| WO1989009219A1 (en) * | 1988-03-24 | 1989-10-05 | The General Hospital Corporation | Artificial chromosome vector |
| WO1995003400A1 (en) * | 1993-07-23 | 1995-02-02 | Johns Hopkins University School Of Medicine | Recombinationally targeted cloning in yeast artificial chromosomes |
-
1994
- 1994-05-25 GB GB9410446A patent/GB9410446D0/en active Pending
-
1995
- 1995-05-25 WO PCT/GB1995/001195 patent/WO1995032297A1/en active Application Filing
- 1995-05-25 AU AU25343/95A patent/AU2534395A/en not_active Abandoned
- 1995-05-25 ZA ZA954300A patent/ZA954300B/en unknown
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