WO1994013789A3 - Oligonucleotides with rna cleavage activity - Google Patents

Oligonucleotides with rna cleavage activity Download PDF

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Publication number
WO1994013789A3
WO1994013789A3 PCT/GB1993/002486 GB9302486W WO9413789A3 WO 1994013789 A3 WO1994013789 A3 WO 1994013789A3 GB 9302486 W GB9302486 W GB 9302486W WO 9413789 A3 WO9413789 A3 WO 9413789A3
Authority
WO
WIPO (PCT)
Prior art keywords
oligonucleotides
cleavage activity
rna cleavage
increased stability
stability against
Prior art date
Application number
PCT/GB1993/002486
Other languages
French (fr)
Other versions
WO1994013789A2 (en
Inventor
Janos Ludwig
Fritz Benseler
Gerd Kotzorek
Original Assignee
Ribonetics Gmbh
Janos Ludwig
Fritz Benseler
Gerd Kotzorek
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ribonetics Gmbh, Janos Ludwig, Fritz Benseler, Gerd Kotzorek filed Critical Ribonetics Gmbh
Priority to AU59741/94A priority Critical patent/AU5974194A/en
Priority to EP94905771A priority patent/EP0672122A1/en
Publication of WO1994013789A2 publication Critical patent/WO1994013789A2/en
Publication of WO1994013789A3 publication Critical patent/WO1994013789A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/318—Chemical structure of the backbone where the PO2 is completely replaced, e.g. MMI or formacetal
    • C12N2310/3183—Diol linkers, e.g. glycols or propanediols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The present invention relates to oligonucleotides with RNA cleavage activity having sequence (I) in which ----X and Y---- are target specific RNA recognition sequences, A, C, G and U are deoxynucleotides and at least one a and/or g is a 2'-substituted adenosine or guanosine derivative respectively, P is either a) A-U or b) C-G and, W is either i) a nucleotide loop sequence or ii) dial bridges connected with phosphodiester or substituted neutral phosphotriester derivative linkages. Oligonucleotides of the invention may exhibit increased stability against RNase degradation, increased stability against exonuclease degradation and facilitated cellular uptake.
PCT/GB1993/002486 1992-12-04 1993-12-03 Oligonucleotides with rna cleavage activity WO1994013789A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU59741/94A AU5974194A (en) 1992-12-04 1993-12-03 Oligonucleotides with rna cleavage activity
EP94905771A EP0672122A1 (en) 1992-12-04 1993-12-03 Oligonucleotides with rna cleavage activity

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9225427.5 1992-12-04
GB929225427A GB9225427D0 (en) 1992-12-04 1992-12-04 Oligonucleotides with rna cleavage activity

Publications (2)

Publication Number Publication Date
WO1994013789A2 WO1994013789A2 (en) 1994-06-23
WO1994013789A3 true WO1994013789A3 (en) 1994-08-04

Family

ID=10726157

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1993/002486 WO1994013789A2 (en) 1992-12-04 1993-12-03 Oligonucleotides with rna cleavage activity

Country Status (4)

Country Link
EP (1) EP0672122A1 (en)
AU (1) AU5974194A (en)
GB (1) GB9225427D0 (en)
WO (1) WO1994013789A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69431669T2 (en) * 1993-09-02 2003-10-23 Ribozyme Pharm Inc ENZYMATIC NUCLEIC ACID THAT CONTAINS NON-NUCLEOTIDS
AU730934B2 (en) * 1993-09-02 2001-03-22 Ribozyme Pharmaceuticals, Inc. Non-nucleotide containing enzymatic nucleic acid
WO1995006764A2 (en) * 1993-09-03 1995-03-09 Vpi Holdings Ltd. Oligonucleotides with rna cleavage activity
JPH10501809A (en) * 1994-06-22 1998-02-17 ネクスター ファーマスーティカルズ，インコーポレイテッド Novel method for producing known and novel 2'-nucleosides by intramolecular nucleophilic substitution
JP2000501284A (en) * 1995-11-14 2000-02-08 ビムラックス ホールディングズ，リミテッド Chimeric oligomer having RNA cleavage activity
US6610478B1 (en) 1996-08-16 2003-08-26 Yale University Phenotypic conversion of cells mediated by external guide sequences
US6737236B1 (en) 1997-01-08 2004-05-18 Proligo, Llc Bioconjugation of macromolecules
US7427678B2 (en) 1998-01-08 2008-09-23 Sigma-Aldrich Co. Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method
WO2003062452A2 (en) 2002-01-23 2003-07-31 Proligo, Llc Methods for the integrated synthesis and purification of oligonucleotides
KR20050006221A (en) * 2002-05-06 2005-01-15 제네랩스 테크놀로지스, 인코포레이티드 Nucleoside derivatives for treating hepatitis c virus infection
WO2004058794A1 (en) 2002-12-31 2004-07-15 Proligo Llc Methods and compositions for the tandem synthesis of two or more oligonuleotides on the same solid support

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991019789A1 (en) * 1990-06-19 1991-12-26 Commonwealth Scientific And Industrial Research Organisation Endonucleases
WO1992007065A1 (en) * 1990-10-12 1992-04-30 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Modified ribozymes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991019789A1 (en) * 1990-06-19 1991-12-26 Commonwealth Scientific And Industrial Research Organisation Endonucleases
WO1992007065A1 (en) * 1990-10-12 1992-04-30 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Modified ribozymes

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BENSELER, F. ET AL: "Hammerhead-like molecules containing non-nucleoside linkers are active RNA catalysts", J. AM. CHEM. SOC., 115 (18),, 8 September 1993 (1993-09-08), pages 8483 - 8484 *
FU, D.-J. & MCLAUGHLIN, L.: "Importance of specific purine amino and hydroxyl groups for efficient cleavage by a hammerhead ribozyme", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 89, no. 9, 1 May 1992 (1992-05-01), WASHINGTON US, pages 3985 - 3989 *
MARRIOTT, J. ET AL.: "synthesis of 2'-thioadenosine", CARBOHYDRATE RESEARCH, vol. 216, 22 September 1991 (1991-09-22), AMSTERDAM NL, pages 257 - 269 *
RANGANATHAN, R. & LARWOOD, D.: "Facile conversion of adenosine into new 2'-substi-tuted-2'-deoxy-arabinofuranosyladenine derivatives: stereospecific synthesis of 2'-azido-2'-deoxy-, 2'-amino-2'-deoxy-, and 2'mercapto--2'-deoxy-beta-D-arabino- furanosyladenines", TETRAHEDRON LETTERS, vol. 45, 1978, OXFORD GB, pages 4341 - 4244 *

Also Published As

Publication number Publication date
EP0672122A1 (en) 1995-09-20
WO1994013789A2 (en) 1994-06-23
AU5974194A (en) 1994-07-04
GB9225427D0 (en) 1993-01-27

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