WO1993018170A1 - Resistance aux anguillules des racines noueuses - Google Patents

Resistance aux anguillules des racines noueuses Download PDF

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Publication number
WO1993018170A1
WO1993018170A1 PCT/GB1993/000514 GB9300514W WO9318170A1 WO 1993018170 A1 WO1993018170 A1 WO 1993018170A1 GB 9300514 W GB9300514 W GB 9300514W WO 9318170 A1 WO9318170 A1 WO 9318170A1
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WO
WIPO (PCT)
Prior art keywords
plant
root knot
gene
molecule
cells
Prior art date
Application number
PCT/GB1993/000514
Other languages
English (en)
Inventor
Howard John Atkinson
Dianna Joy Bowles
Sarah Jane Gurr
Michael John Mcpherson
Original Assignee
Advanced Technologies (Cambridge) Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advanced Technologies (Cambridge) Ltd. filed Critical Advanced Technologies (Cambridge) Ltd.
Priority to TJ96000329A priority Critical patent/TJ287B/xx
Priority to AU36455/93A priority patent/AU3645593A/en
Priority to MD96-0266A priority patent/MD1400C2/ro
Publication of WO1993018170A1 publication Critical patent/WO1993018170A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Definitions

  • the subject invention relates to resistance in plants to deleterious effects of infection by root knot nematode.
  • Root knot nematodes are major pathogens of many crop plants, for example vegetables, food legumes, tobacco, tomato, water melon, grape, peanut and cotton.
  • SUBSTITUTE SHEET root meristem Pharyngeal gland secretions are then injected through the stylet of the nematode into cells in the region of the meristem. This causes the normal development of these cells to be disrupted, whereby nuclear division occurs without the occurrence of cell division. There are thus formed multi-nucleate cells, known as "giant cells'*.
  • giant cells'* multi-nucleate cells
  • hypertrophic cells which the nematode does not attack directly by stylet penetration.
  • the giant cells and the surrounding hypertrophic cells together constitute the feeding site of root knot nematodes.
  • the observed knot formed on the infected root consists of such giant cells and the accompanying hypertrophic cells which cure the result of a multiplicity of nematode infections.
  • the mechanism of the production of giant cells is similar in all susceptible plant species.
  • the root knot nematode loses locomotory ability as feeding by the nematode on the giant cell proceeds, and the nematode becomes committed to feeding, development and reproduction at the feeding site.
  • SUBSTITUTE SHEET the syncytium of a plant which has been infected by the root cyst nematode.
  • the subject invention provides a method of producing root knot nematode resistant plants, wherein in respect of a root knot nematode infected plant there is identified a gene which is expressed in the giant cells and/or the accompanying hypertrophic cells of root knots of the plant, the promoter of said gene is taken and fused with a coding sequence to provide a chimaeric gene which encodes a molecule which is inimical to one or more of 1. root knot giant cells, 2. root knot hypertrophic cells and 3. root knot nematodes, and a further plant is transformed with said chimaeric gene.
  • Plants to which may be imparted root knot nematode resistance in accordance with the subject invention include vegetable plants, food legumes, tobacco, edible fruit plants, edible nut plants and cotton.
  • the subject invention may be applied to carrot plants and in respect of fruit plants it may be applied to tomato plants.
  • the inventive method is, in fact, applicable to all such species which are also transformable in accordance with the transformation step of the method.
  • the inventive method is applicable in respect of Meloidogyne species including but not limited to M. incognita, M.javanica, M.arenaria and M.hapla.
  • SUBSTITUTE SHEET The gene identified and selected from an infected plant is preferably one the expression of which takes place not before the nematode has substantially lost loc ⁇ motory ability.
  • Sequences (in the chimaeric gene) to be expressed under the control of the said promoter include one or more of:-
  • a coding sequence for a molecule that causes necrosis of giant cells and/or hypertrophic cells 1.
  • a coding sequence for a molecule that causes necrosis of a root knot nematode 1.
  • Antisense of the coding sequence for enzymes critical to plant cell metabolism is essential to plant cell metabolism.
  • root knot nematode resistance can be imparted to plants without the need to produce constitutively an anti nematode infection product as listed above at 1-5.
  • Seeds of C319 tobacco are germinated on Fisons Fl compost under conditions as follows. Light intensity of 4500 to 5000 lux, with 16 hour periods of light alternating with 8 hour periods of darkness, and temperatures between 20°C and 25°C. After c. 3 weeks seedlings are gently washed in tap water to remove soil and transferred to pouches (2 plants per pouch; Northrup- King) and grown for a further week in a Conviron at 25°C and with a light intensity of 5500 lux for 16 hour periods alternating with 8 hour periods of darkness. Roots are lifted from the back of the pouch and supported with Whatman GF/A glass fibre paper at their tips. Three day old nematodes (M.
  • javanica javanica
  • lO ⁇ l 50 nematodes
  • a second piece of GF/A paper is placed on top to fully encapsulate the root tip.
  • the GF/A paper is removed to ensure synchronous infection.
  • the knots are dissected out (leaving healthy root and root tip tissue behind) and frozen immediately in liquid nitrogen.
  • Approximately 0.5 to Ig of infected root tissue can be harvested from 80 inoculated plants. Staining for visualisation of nematodes in infected roots To establish the quality of the infection the number of nematodes (infecting) per root tip is determined. Roots are harvested from 3 day post infected plants and
  • Root tissue is ground to a fine powder in a chilled (liquid nitrogen) pestle and mortar. About lOO g aliquots are then transferred to similarly chilled Eppendorf tubes and 300 ⁇ l of hot phenol extraction buffer added (50% phenol, 50% extraction buffer : 0.1M lithium chloride, 0.1M Tris-HCl pH8.0 (RT) , lOmH EDTA, 1% SDS) and incubated at 80 ⁇ C for 5 ins. An equal volume of chloroform is then added and the homogenate microfuged for 15 minutes at 4*C. The aqueous phase is then extracted with 600 ⁇ l of phenol/chloroform and microfuged as above.
  • hot phenol extraction buffer 50% phenol, 50% extraction buffer : 0.1M lithium chloride, 0.1M Tris-HCl pH8.0 (RT) , lOmH EDTA, 1% SDS
  • RNA quality is assessed by denaturing gel electrophoresis. (Adapted from Shirzadegan et al 1991) . Subtractive clonin ⁇ of infection specific cDNAs
  • Poly(A) + RNA is isolated from 200 ⁇ g total RNA samples from healthy and infected C319 root tissue using magnetic oligo dT Dynabead ⁇ according to the
  • First strand cDNA synthesis is performed in situ on the Dynabead bound poly (A) + fraction from the healthy tissue. This is the Driver DNA.
  • First and second strand synthesis is performed in situ on the Dynabead bound poly (A) + fraction from the infected tissue. This is the Target DNA. All cDNA reactions are carried out using Pharmacia's cDNA synthesis kit and according to the manufacturer's instructions.
  • oligonucleotides SUB21 (5 • CTCTTGCTTGAATTCGGACTA3•) ,SUB25(5 » TAGTCCGAATTCAAGCAAGAG CACA3') (sequences from Duguid & Dinauer, 1990) and LDT15 (5'GACAGAAGCGGATCCd(T) 15 3') (O'Reilly et al , 1991) are kinased with T4 polynucleotide kinase according to Maniatis et al, (1982). SUB21 and SUB25 are then annealed to form a linker which is then ligated to the target DNA with T4 DNA ligase according to King & Blakesley (1986). Following this, the beads carrying the Target are washed extensively with TE and the second strand of the cDNA eluted at 95°C in 5xSSC.
  • RNA bound to the Dynabead bound Driver DNA is removed by heat and the eluted Target DNA hybridised to the Driver DNA at 55 ⁇ C in 5 x SSC for 5 hours.
  • Non- hybridising Target DNA is separated from the bead bound driver DNA at room temperature following the manufacturer's instructions, following which, hybridising Target DNA is similarly separated from the bead bound Driver DNA at 95°C.
  • the RT eluted Target DNA is then added back to the Driver DNA and the hybridisation repeated. This process is repeated until the amount of
  • Recombinants are identified by colony PCR (Gussow & Clackson, 1989) .
  • the amplified inserts are Southern blotted in triplicate onto Pall Biodyne membranes as described by the membrane manufacturer. Prehybridisation and hybridisation are carried out with the same temperature and buffer which are 42°C and 5 x SSPE,0.05% BLOTTO,50% for amide. These are hybridised separately to cDNA probes (see below) from healthy and infected tissue and to a probe comprising amplified Target DNA from the final subtraction. Clones that show a hybridisation signal to the infected cDNA probe only or that show a hybridisation signal to the subtracted probe but not the cDNA probes are selected for further analysis.
  • cDNA synthesis is conducted 'cold' on total RNA and the synthesis products then labelled by oligolabelling.
  • Samples of lO ⁇ g total RNA from healthy and infected tissue are first treated with 2.5 units DNase l at 37°C for 15 minutes. The DNase is then denatured at 95°C for 10 minutes before cDNA synthesis is performed (standard Pharmacia protocol) .
  • the RNA is then removed in the presence of 0.4M sodium hydroxide for 10 minutes at RT and the DNA purified through a spun Sephacryl 400HR column.
  • cDNA yield and concentration are determined using DNA Dipsticks (Invitrogen) .
  • the cDNA products are then labelled as for Pharmacia's standard oligolabelling protocol (c. 35ng/probe) .
  • RNA blots comprise 25 ⁇ g RNA per lane whilst poly (A)+ blots comprise 0.5 to l ⁇ g RNA per lane.
  • the RNA is electrophoresed on formaldehyde gels and blotted onto Pall Biodyne B membrane as described by Fourney et al (1988) . Probes are labelled and hybridised to blots as described above.
  • C 19 and M.javanica DNA are prepared as described by Gawel & Jarret, (1991) .
  • Southern blots are prepared comprising lO ⁇ g Ec ⁇ RL and Hindlll digested DNA per lane. The blots are hybridised to oligolabelled probes as described above. In situ hybridisations
  • RNAs of interest are determined prior to the isolation of their promoter sequences. This is achieved by using 5' RACE as described by Frohman et al, (1988). Isolation of promoter regions
  • the promoter regions of the genes of interest cure isolated by a process termed Vector-Ligated PCR.
  • lOOng samples of restriction endonuclease digested C319 genomic DNA are ligated for 4 hours at RT (King & Blakesley, 1986) with lOOng samples of pBluescript (digested with a restriction enzyme producing compatible termini) .
  • enzymes used are Ec ⁇ KL, Bam ⁇ l, Hindlll, BG111, Xhol, Clal, Sail, Kpnl, Pstl, and Sstl.
  • PCR is then performed on the ligations using a vector primer such as the -40 Sequencing primer and a primer complementary to
  • SUBSTITUTE SHEET the 5' terminus of the mRNA.
  • the PCR products are then cloned and sequenced. If necessary, the process is repeated with a new primer complementary to the 5' terminus of the promoter fragment to ensure that the control sequences of the promoters are isolated. Construction of chimaeric genes in binary plant transformation vectors
  • the isolated promoters are ligated 5' to a sequence which is a sequence of one of the classes 1. - 5. as detailed hereinabove, examples being the antisense of the gene itself (class 4.) or the barnase gene (Hartley et al, 1972) (class 1. and/or class 3.). These are constructed in binary vectors (Bevan, 1984) .
  • Transgenic plants for example tobacco, may be produced by the standard Agrrobacteriiun mediated leaf disc method described by Horsch et al (1985) , thus to provide root knot nematode resistant plants. Seeds or other propagules of plants the product of the subject invention can be stored for future use.
  • SUBSTITUTE SHEET impaired so that the population of root knot nematodes in the soil at the location of the plants is reduced to an economically insignificant size.
  • Root knot nematode resistance may be imparted in accordance with the subject invention to all root knot nematode susceptible monocotyledonous, dicotyledonous, herbaceous and woody plant species. References ⁇

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Botany (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Afin de produire des plantes résistantes aux anguillules des racines noueuses, on identifie un gène exprimé au niveau du site d'alimentation des anguillules dans une plante infectée. On isole le promoteur du gène du site d'alimentation et on l'associe à une séquence codant une molécule hostile à l'infection par les anguillules des racines noueuses. On transforme une autre plante supplémentaire à l'aide du gène chimérique ainsi obtenu.
PCT/GB1993/000514 1992-03-13 1993-03-11 Resistance aux anguillules des racines noueuses WO1993018170A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
TJ96000329A TJ287B (en) 1992-03-13 1993-03-11 A method of producing root knot nematode resistant plants
AU36455/93A AU3645593A (en) 1992-03-13 1993-03-11 Root knot nematode resistance
MD96-0266A MD1400C2 (ro) 1992-03-13 1993-03-11 Procedeu de producere a plantelor rezistente la nematodul galicol javanez, plantă obţinută prin procedeul indicat, mlădiţă a ei şi genă himeră

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9205474.1 1992-03-13
GB929205474A GB9205474D0 (en) 1992-03-13 1992-03-13 Root knot nematode resistance

Publications (1)

Publication Number Publication Date
WO1993018170A1 true WO1993018170A1 (fr) 1993-09-16

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Application Number Title Priority Date Filing Date
PCT/GB1993/000514 WO1993018170A1 (fr) 1992-03-13 1993-03-11 Resistance aux anguillules des racines noueuses

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CN (1) CN1080302C (fr)
CZ (1) CZ289067B6 (fr)
GB (1) GB9205474D0 (fr)
GE (1) GEP20002245B (fr)
MD (1) MD1400C2 (fr)
MY (1) MY109599A (fr)
NZ (1) NZ267026A (fr)
TJ (1) TJ287B (fr)
TR (1) TR28954A (fr)
WO (1) WO1993018170A1 (fr)

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0612208A1 (fr) * 1991-10-04 1994-08-31 North Carolina State University Plantes transgeniques resistant a des agents pathogenes
US5612471A (en) * 1994-05-25 1997-03-18 The Regents Of The University Of California Nematode-induced genes in tomato
WO1997046692A1 (fr) * 1996-06-04 1997-12-11 Mogen International N.V. Promoteur de gene de plante inductible par nematodes
EP0823481A1 (fr) * 1996-08-09 1998-02-11 Keygene N.V. Résistance contre les nématodes
WO1998006750A2 (fr) * 1996-08-09 1998-02-19 Keygene N.V. Resistance aux nematodes et/ou aux aphides
US6008436A (en) * 1993-01-21 1999-12-28 North Carolina State University Nematode-resistant transgenic plants
US6262344B1 (en) 1995-06-13 2001-07-17 Syngenta Mogen B.V. Nematode-inducible plant gene promoter
US6392119B1 (en) 1997-01-24 2002-05-21 Dna Plant Technology Corporation Two component plant cell lethality methods and compositions
US7282624B2 (en) 2000-10-14 2007-10-16 Advanced Technologies (Cambridge) Limited Plant cell death system
WO2012059497A1 (fr) 2010-11-02 2012-05-10 Bayer Cropscience Ag N-hétarylméthyl pyrazolylcarboxamides
WO2012089757A1 (fr) 2010-12-29 2012-07-05 Bayer Cropscience Ag Dérivés d'hydroxymoyl-tétrazole fongicides
US8722072B2 (en) 2010-01-22 2014-05-13 Bayer Intellectual Property Gmbh Acaricidal and/or insecticidal active ingredient combinations
WO2014090765A1 (fr) 2012-12-12 2014-06-19 Bayer Cropscience Ag Utilisation de 1-[2-fluoro-4-méthyle-5-(2,2,2- trifluoroéthylsulfinyl)phényl]-5-amino-3-trifluorométhyl)-1 h-1,2,4 tfia zole à des fins de régulation des nématodes dans les cultures résistantes aux nématodes
US9265252B2 (en) 2011-08-10 2016-02-23 Bayer Intellectual Property Gmbh Active compound combinations comprising specific tetramic acid derivatives
US9867378B2 (en) 2012-12-13 2018-01-16 Instituto De Ecologia, A.C. Biocontrol of phytoparasitic nematodes by paecilomyces
US10844390B2 (en) 2015-08-07 2020-11-24 Basf Agricultural Solutions Seed, Us Llc Root-preferential and stress inducible promoter and uses thereof

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1317383C (zh) * 2005-03-16 2007-05-23 云南大学 具有杀线虫功能的囊孢单顶孢菌剂及其制备方法和应用
CN100372935C (zh) * 2005-10-17 2008-03-05 华中农业大学 辣椒抗根结线虫基因的克隆及其应用
MD719Z (ro) * 2013-06-11 2014-08-31 Институт Зоологии Академии Наук Молдовы Procedeu de tratare a cartofului contra nematodului Ditylenchus destructor

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EP0298918A2 (fr) * 1987-07-10 1989-01-11 Ciba-Geigy Ag Résistance inductible contre des virus dans des plantes
WO1992004453A1 (fr) * 1990-09-10 1992-03-19 The University Of Leeds Lutte contre les nematodes parasites des vegetaux
WO1992021757A1 (fr) * 1991-05-30 1992-12-10 Plant Genetic Systems, N.V. Promoteurs destines aux plantes et reagissant aux nematodes
WO1993006710A1 (fr) * 1991-10-04 1993-04-15 North Carolina State University Plantes transgeniques resistant a des agents pathogenes
WO1993010251A1 (fr) * 1991-11-20 1993-05-27 Mogen International N.V. Procede de production de plantes a sensibilite reduite aux nematodes parasites des plantes

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CN1033645A (zh) * 1988-10-22 1989-07-05 中国科学院上海植物生理研究所 控制植物病毒病的基因工程方法

Patent Citations (5)

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Publication number Priority date Publication date Assignee Title
EP0298918A2 (fr) * 1987-07-10 1989-01-11 Ciba-Geigy Ag Résistance inductible contre des virus dans des plantes
WO1992004453A1 (fr) * 1990-09-10 1992-03-19 The University Of Leeds Lutte contre les nematodes parasites des vegetaux
WO1992021757A1 (fr) * 1991-05-30 1992-12-10 Plant Genetic Systems, N.V. Promoteurs destines aux plantes et reagissant aux nematodes
WO1993006710A1 (fr) * 1991-10-04 1993-04-15 North Carolina State University Plantes transgeniques resistant a des agents pathogenes
WO1993010251A1 (fr) * 1991-11-20 1993-05-27 Mogen International N.V. Procede de production de plantes a sensibilite reduite aux nematodes parasites des plantes

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Title
CHEMICAL ABSTRACTS, vol. 113, 1990, Columbus, Ohio, US; abstract no. 127723, JUN, W. 'Preparation of transgenic plants for the control of virosis' *
THE PLANT JOURNAL vol. 1, no. 2, September 1991, pages 245 - 254 SIJMONS, P.C., ET AL. 'Arabidopsis thaliana as a new model host for plant parasitic nematodes' *

Cited By (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0612208A4 (fr) * 1991-10-04 1995-07-12 Univ North Carolina Plantes transgeniques resistant a des agents pathogenes.
US5750386A (en) * 1991-10-04 1998-05-12 North Carolina State University Pathogen-resistant transgenic plants
EP0612208A1 (fr) * 1991-10-04 1994-08-31 North Carolina State University Plantes transgeniques resistant a des agents pathogenes
US6008436A (en) * 1993-01-21 1999-12-28 North Carolina State University Nematode-resistant transgenic plants
US5612471A (en) * 1994-05-25 1997-03-18 The Regents Of The University Of California Nematode-induced genes in tomato
US6262344B1 (en) 1995-06-13 2001-07-17 Syngenta Mogen B.V. Nematode-inducible plant gene promoter
WO1997046692A1 (fr) * 1996-06-04 1997-12-11 Mogen International N.V. Promoteur de gene de plante inductible par nematodes
EP1493817A1 (fr) * 1996-08-09 2005-01-05 Keygene N.V. La résistance contre les nuisibles
EP0823481A1 (fr) * 1996-08-09 1998-02-11 Keygene N.V. Résistance contre les nématodes
WO1998006750A2 (fr) * 1996-08-09 1998-02-19 Keygene N.V. Resistance aux nematodes et/ou aux aphides
WO1998006750A3 (fr) * 1996-08-09 1998-06-25 Keygene Nv Resistance aux nematodes et/ou aux aphides
US6613962B1 (en) 1996-08-09 2003-09-02 Keygene N.V. Tomato nucleic acid encoding protein that confers resistance to aphids and nematodes and plants transformed therewith
US6392119B1 (en) 1997-01-24 2002-05-21 Dna Plant Technology Corporation Two component plant cell lethality methods and compositions
US7282624B2 (en) 2000-10-14 2007-10-16 Advanced Technologies (Cambridge) Limited Plant cell death system
US8722072B2 (en) 2010-01-22 2014-05-13 Bayer Intellectual Property Gmbh Acaricidal and/or insecticidal active ingredient combinations
WO2012059497A1 (fr) 2010-11-02 2012-05-10 Bayer Cropscience Ag N-hétarylméthyl pyrazolylcarboxamides
WO2012089757A1 (fr) 2010-12-29 2012-07-05 Bayer Cropscience Ag Dérivés d'hydroxymoyl-tétrazole fongicides
US9265252B2 (en) 2011-08-10 2016-02-23 Bayer Intellectual Property Gmbh Active compound combinations comprising specific tetramic acid derivatives
WO2014090765A1 (fr) 2012-12-12 2014-06-19 Bayer Cropscience Ag Utilisation de 1-[2-fluoro-4-méthyle-5-(2,2,2- trifluoroéthylsulfinyl)phényl]-5-amino-3-trifluorométhyl)-1 h-1,2,4 tfia zole à des fins de régulation des nématodes dans les cultures résistantes aux nématodes
US9867378B2 (en) 2012-12-13 2018-01-16 Instituto De Ecologia, A.C. Biocontrol of phytoparasitic nematodes by paecilomyces
US10844390B2 (en) 2015-08-07 2020-11-24 Basf Agricultural Solutions Seed, Us Llc Root-preferential and stress inducible promoter and uses thereof

Also Published As

Publication number Publication date
CN1077990A (zh) 1993-11-03
TJ287B (en) 2000-12-13
MD960266A (en) 1998-01-31
MY109599A (en) 1997-03-31
CZ289067B6 (cs) 2001-10-17
TR28954A (tr) 1997-08-04
CN1080302C (zh) 2002-03-06
NZ267026A (en) 1995-08-28
GEP20002245B (en) 2000-09-25
MD1400C2 (ro) 2000-10-31
GB9205474D0 (en) 1992-04-29
MD1400B2 (en) 2000-01-31
CZ210394A3 (en) 1997-05-14

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