WO1993006208A1 - Enterococcus microencapsule dans un acide gras destine a la volaille - Google Patents

Enterococcus microencapsule dans un acide gras destine a la volaille Download PDF

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Publication number
WO1993006208A1
WO1993006208A1 PCT/US1992/007589 US9207589W WO9306208A1 WO 1993006208 A1 WO1993006208 A1 WO 1993006208A1 US 9207589 W US9207589 W US 9207589W WO 9306208 A1 WO9306208 A1 WO 9306208A1
Authority
WO
WIPO (PCT)
Prior art keywords
probiotic
fatty acid
feed
poultry
bacteria
Prior art date
Application number
PCT/US1992/007589
Other languages
English (en)
Inventor
William M. Rutherford
Jack E. Allen
Scott M. Dennis
Mark A. Hinds
Gregory R. Dana
Original Assignee
Pioneer Hi-Bred International, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi-Bred International, Inc. filed Critical Pioneer Hi-Bred International, Inc.
Priority to SK324-94A priority Critical patent/SK278992B6/sk
Priority to BR9206505A priority patent/BR9206505A/pt
Priority to EP19920920241 priority patent/EP0604543A4/en
Priority to RO94-00449A priority patent/RO113477B1/ro
Priority to RU9294019485A priority patent/RU2093571C1/ru
Priority to JP5506091A priority patent/JPH06511148A/ja
Priority to CS94595A priority patent/CZ280601B6/cs
Publication of WO1993006208A1 publication Critical patent/WO1993006208A1/fr
Priority to BG98665A priority patent/BG62175B1/bg

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/46—Streptococcus ; Enterococcus; Lactococcus

Definitions

  • Growth enhancers in the form of antibiotics have been used extensively for poultry, namely chickens and turkey.
  • Growth enhancers such as Stafac® and BMD ® (bacitracin methylene disalicylate) are known antibiotics and have been used at sub-therapeutic levels of for example, 10 grams per ton and 25 grams per ton as feed additives in order to promote
  • probiotics do have some difficulty in maintaining a stable product. Typically, the probiotic is used at a fairly low level, added to feed at perhaps a 0.1% level. However, unused probiotic containing feed or feed additive product is often stored by the farmers for long periods of time. This storage many times is under conditions where there is some moisture and high temperature. In many instances there is just enough moisture that the bacteria are activated or start to grow, but yet there is an insufficient amount of moisture to sustain them. As a result they die. Thus, the activity of the probiotic is stopped. In other instances, the addition of antibiotics to the probiotic containing feed or feed additive adversely interacts with the bacteria, particularly if there are small amounts of moisture present and thus again bacteria are killed. Thus, there is a significant problem of long term storage stability for
  • Certain features of poultry are especially desirable to achieve if possible. Those include an increased rate of weight gain, better feed
  • uniformity of flock weight is important because the most desirable area for tissue deposit is the breast in order to yield a high amount of choice meat. Thus, weight gain is not only important, but where the weight is gained on the carcass is also important. Uniformity of flock weight is important because if more birds are normal in size, less hand labor is required and processors can more extensively rely on machine processing. On the other hand, if the birds vary considerably from very small birds to very large birds, even though the overall flock weight may be the same, the smaller birds and the larger birds require a great deal more hand labor and because of their lack of uniformity in size, cannot be processed easily by machine. Thus, uniformity of flock weight with a high percentage distribution within the normal size range so that chickens can process by standardized machinery is a desirable feature.
  • inventions to provide a poultry probiotic which contains no antibiotics and contains only fatty acid microencapsulated naturally occurring organisms.
  • DSM is a Bacterial Culture collection in Germany. DMS stands for Deutsche Sammlung von Mikroorganismen located in Braunschweig, West
  • invention to provide a probiotic which, for poultry, provides increased rate of weight gain, which
  • An even further primary objective of the present invention is to provide probiotics suitable for poultry feed ration addition which contains bacteria that are in microsphere form using a special rotary technique using free fatty acid matrix.
  • Another objective of the present invention is to provide a probiotic which has stability at levels within the range of from 3 months to 6 months without any significant organism count reduction.
  • Another objective of the present invention is to provide a process of rotary formation of spheres of the dried bacteria which provides having uniform size.
  • Another objective of the present invention is to provide rotary disc spheres of dried bacteria which are free flowing, and easily processable with poultry feed rations.
  • Figure 4 is a graph showing breast yield
  • Figure 5 is a graph showing body weight
  • Figures 4 and 5 show a control, use of an
  • the invention is a method and composition of growth promotion for poultry which comprises adding to the normal poultry feed ration a small but growth promoting effective amount of a probiotic which contains dried, fatty acid microspheres of
  • Enterococcus faecium 301 DSM No. DSM-Nr. 4789, and dried fatty acid microepheres of Enterococcus faecium 202, DSM No. DSM-Nr. 4788, where preferably the fatty microspheres are formed by rotary disc drying.
  • a fatty acid employed may be any one of the C 12 to C 24 free fatty acids, but is preferably stearic acid.
  • the organisms are preferably present in about equal amounts but may vary within the range from about 30% to about 70% of one of the organisms with the balance being the other.
  • organisms provide the desirable features of the present invention, namely increased rate of weight gain, better feed conversion, increased yield of breast meat, and increased uniformity of flock weight.
  • the fact is that they do, provided that both are used in combination so that they can somehow interact with each other, and providing that they are used within the range herein expressed. It is these combinations of features which some how interact and co-act to provide the desirable features of the present invention which allow significantly improved poultry carcass, meat quality and processing.
  • the amount of probiotic added to the feed ration can vary considerably but generally will be within the range of from about 0.5 pounds to about 2.0 pounds per ton of feed, generally from about 0.8 pounds to about 1.2 pounds per ton of feed, and typically at about 1 pound per ton of feed.
  • the organism count, that is the number of colony forming units per gram present in the probiotic can also vary within the range of from about 1 x 10 6 CFU/gm to about 2 x 10 9 CFU/gm, but is preferably at about 2 x
  • Growth promoters now used include antibotics such as
  • the method of processing of the organisms is not critical as long as the organisms can be kept alive to delivery to the animal, and placed in a form so that it will combine with animal feed well and is of a generally uniform size so that dosage may be controlled.
  • microspheres are formed wherein each sphere constitutes a plurality of bacteria in a free fatty acid matrix rather than an individual microencapsulator of each bacteria in a coating or film like layer of fatty acid.
  • the preferred encapsulating agent is a C 12 to C 24 free fatty acid. While mixtures of fatty acids may be employed, it is preferred that a single pure free fatty acid be employed. It is also preferred that the free fatty acid be an unsaturated fatty acid, with the most preferred being stearic acid.
  • the fatty acid have a melting point less than 75°C, preferably within the range of 40°C to 75°C. It must, of course, be solid at room temperature in order to be an effective matrix. All free fatty acids falling within the range of chemical
  • the bacteria are typically freeze-dried bacteria as placed in the product. Thus, they can be revived by moisture addition.
  • the microspheres generally comprise from about 50% to over 90% by weight of the fatty acid component with the balance being bacterial culture.
  • the preferred range is from about 60% to about 75% fatty acid. If too little fatty acid is used, the matrix will be inadequate for protection. On the other hand, if too much is used, the matrix will be too thick and results in inadequate release in the gut.
  • the process as used in this invention is a rotary disc microsphere formation process.
  • a slurry of the bacteria and fatty acid components are thoroughly mixed with the mixture being added at a uniform rate onto the center of a rotating stainless steel disc. It is there flung outwardly as a result of
  • centrifugal force and forms a microsphere. It is then collected in a cooling chamber maintained at ambient conditions or slightly lower, sized and readied for packaging.
  • Microencapsulation provides a shell coating around an object, and bacteria have proven to be too small, too hard to keep alive or provide in a uniform size to be of practical usefulness. With microsphere formation, particularly with agents used in this invention is used, the stability of the resulting bacteria, even when subjected to some moisture and antibiotics, will be for from three to six months with the
  • the rotary disk typically employing a 4"-6" rotary disc
  • the rotary disk can be run at the rate of from 2000 rpm to 4000 rpm, preferably about 2500 rpm to 3200 rpm with a feed rate of from 50 grams to 200 grams per minute.
  • the preferred conditions presently known are use of stearic acid, use of two hereinbefore described organisms, a four inch rotary disc, 3000 rpm and a feed rate of 100 grams per minute with a bacteria/stearic acid slurry of 35% bacteria, 65% stearic acid.
  • a product having a particle size of from 75 microns to 300 microns will be achieved, with a preferred level of less than 250 microns.
  • Example 1 through 4 and Figures 1, 2, and 3 relate to the invention of my prior case.
  • Example 5, and tables 2-10 relate to the process of this present invention for a poultry probiotic.
  • Example 1 correlates with Figure 1. It shows the product stability of two different strains of
  • CFU colony forming unit
  • Example 2 is to be interpreted in connection with Figure 2.
  • the figure shows the stability of individual microsphered strains when mixed in a typical feed ration in the presence of three poultry antibiotics.
  • the ration consisted of the following:
  • Example 3 is to be interpreted in conjunction with Figure 3. It shows the stability of the
  • Enterococcus faecium microspheres in feed in the presence of different antibiotics The ration consisted of 60% fine cracked corn, 38% soybean meal and 2% limestone with a moisture content of about 14%. Culture was added to a level of approximately 10 CFU/gm feed and mixed. Ten pound aliquots were stored in sealed bags at 20 C and sampled weekly for 16 weeks. The antibiotics were included in the ration at the following levels:
  • Table 1 is a list of the minimum times for a 1og loss in colony forming units (CFU). Table 1
  • Example 4 the stability of product after pelletizing for use of a chicken feed product was determined.
  • the microsphere formation conditions were as earlier described.
  • the conditions used in this study were the following:
  • Pellets were stored in unsealed bags and sampled weekly for CFU determination.
  • the pelletized product was not adversely affected in stability by the conditions of pelletizing.
  • the pelletized product showed stability equal to the unpelletized product.
  • Control, M a selected, encapsulated probiotic cultures containing Enterococcus faecium 301, DSM No.
  • DSM-Nr. 4788 each rotary disc fatty acid encapsulated as described in Example 1 and each present as 50% of the probiotic applied at 1 x 10 5 CFU/g of feed, mash
  • a mixer test was conducted for each production phase. The test was designed to ensure that the probiotic was uniformly distributed at appropriate levels in the feed and that it survived pelleting. Each batch was sampled at the time of bagging with 4 equally spaced samples for the mash treatments and 10 equally spaced samples for the pelleted treatments (i.e. bags 1, 3, 5,..., 35, 37, and 39).
  • Alternate floor pens within a treatment had non- contaminated feed sampled during weeks 1 and 4; with the remaining pens sampled on weeks 2 and 6 during the feeding study.
  • Probiotic regardless of processing, improved (P ⁇ .05) feed conversion over the respective Control while increasing (P ⁇ .05) weight gain over the Control only in the mash feed (Table 4).
  • the product was at its desired level and strain composition (Table 5).
  • Probiotic was uniformly distributed within the feed. Probiotic, M was at its desired level while probiotic, P was 1 to 1-1/2 log higher than desired for the starter and grower rations ( Table 6). The high counts for probiotic, P were a result of
  • Pelleting increased the average breast weight by 15 g over mash.
  • Probiotic increased the average breast weight and uniformity (Figure 4) over the Control with the greatest improvement found in mash.
  • Stafac® 10 showed the greatest improvement in uniformity for the pelleted feeds.
  • probiotic treatments produced a shorter (P>.05) small intestinal length than either of the Controls and Stafac® when expressed as actual length, a ratio of either body weight, or breast weight (Table 9).
  • Probiotic, M had a lighter (P>.05) small intestinal weight than Control, M when
  • probiotic, P treated birds produced no off- flavor when compared to Stafac® 10 (Table 10).
  • probiotic, P was perceived to have enhanced the flavor of the thigh/leg when compared to Control, P.
  • this enhancement of flavor was not observed in the first trial.
  • Pen size 4.2' x 15.5' one tube feeder, one hanging waterer, pine shavings on dirt, power and evaporative cooling system and well insulated, forced hot-air heat, curtain sidewall building.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Polymers & Plastics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Food Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Birds (AREA)
  • Animal Husbandry (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Fodder In General (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Des microsphères d'acide gras, séchées sur plateau rotatif, contenant Enterococcus faecium, souches 301 et 202, sont mélangées et utilisées comme additif alimentaire pour la volaille en vue de favoriser la croissance et d'améliorer la qualité de la carcasse.
PCT/US1992/007589 1991-09-20 1992-09-09 Enterococcus microencapsule dans un acide gras destine a la volaille WO1993006208A1 (fr)

Priority Applications (8)

Application Number Priority Date Filing Date Title
SK324-94A SK278992B6 (sk) 1991-09-20 1992-09-09 Probiotická zmes na podporu rastu hydiny
BR9206505A BR9206505A (pt) 1991-09-20 1992-09-09 Enterococcus microencapsulado em ácido graxo para uso com aves.
EP19920920241 EP0604543A4 (en) 1991-09-20 1992-09-09 Fatty acid microencapsulated enterococcus for use with poultry.
RO94-00449A RO113477B1 (ro) 1991-09-20 1992-09-09 Metodă pentru stimularea creşterii păsărilor de curte
RU9294019485A RU2093571C1 (ru) 1991-09-20 1992-09-09 Способ стимулирования роста домашней птицы и препарат на основе пробиотика
JP5506091A JPH06511148A (ja) 1991-09-20 1992-09-09 家禽用脂肪酸マイクロカプセル化腸球菌
CS94595A CZ280601B6 (cs) 1991-09-20 1992-09-09 Způsob podpory růstu drůbeže a enterokoky mikrozapouzdřené mastnými kyselinami pro použití u drůbeže
BG98665A BG62175B1 (bg) 1991-09-20 1994-03-17 Метод и пробиотик за стимулиране растежа на птици

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US76317591A 1991-09-20 1991-09-20
US07/763,175 1991-09-20

Publications (1)

Publication Number Publication Date
WO1993006208A1 true WO1993006208A1 (fr) 1993-04-01

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ID=25067078

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Application Number Title Priority Date Filing Date
PCT/US1992/007589 WO1993006208A1 (fr) 1991-09-20 1992-09-09 Enterococcus microencapsule dans un acide gras destine a la volaille

Country Status (12)

Country Link
EP (1) EP0604543A4 (fr)
JP (1) JPH06511148A (fr)
BG (1) BG62175B1 (fr)
BR (1) BR9206505A (fr)
CA (1) CA2116525A1 (fr)
CZ (1) CZ280601B6 (fr)
HU (1) HUT67466A (fr)
MX (1) MX9205329A (fr)
RO (1) RO113477B1 (fr)
RU (1) RU2093571C1 (fr)
SK (1) SK278992B6 (fr)
WO (1) WO1993006208A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0628072A1 (fr) * 1992-02-26 1994-12-14 Pioneer Hi-Bred International, Inc. Microspheres de micro-organismes sechees fabriquees selon un procede a disques rotatifs
EP0631616A1 (fr) * 1992-03-17 1995-01-04 Pioneer Hi-Bred International, Inc. Microspheres a base d'acide gras, contenant des anterocoques et dstinees a favoriser la croissance et a ameliorer la quantite de la carcasse
WO1997045530A1 (fr) * 1996-05-27 1997-12-04 UZILOVA, Irina Semenovna, Heiress of UZILOV Utilisation de souches de streptococcus faecium et composition a base de ces souches
WO2003071883A1 (fr) 2002-02-28 2003-09-04 Centro Sperimentale Del Latte S.P.A. Compositions dietetiques et/ou pharmaceutiques destinees a l'humain et/ou l'animal, a base de preparations microbiennes probiotiques
WO2005009139A1 (fr) * 2003-07-30 2005-02-03 Chr. Hansen A/S Produit d'animaux d'elevage contenant une bacterie probiotique enterococcus

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2541389C1 (ru) * 2013-07-16 2015-02-10 Государственное научное учреждение Северо-Кавказский зональный научно-исследовательский ветеринарный институт (ГНУ СКЗНИВИ) Российской академии сельскохозяйственных наук Способ стимуляции роста птицы

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3959493A (en) * 1971-03-17 1976-05-25 Rumen Chemie, Ag Rumen bypass products comprising biologically active substances protected with aliphatic fatty acids
GB2016043A (en) * 1978-03-08 1979-09-19 Danochemo As Bacteria-containing product for use in animal feeds, and its production
US4675140A (en) * 1984-05-18 1987-06-23 Washington University Technology Associates Method for coating particles or liquid droplets
US4710379A (en) * 1984-06-19 1987-12-01 Kabushiki Kaisya Advance Kaihatsu Kenkyujo Intestinal microflora-improving agent
US4713245A (en) * 1984-06-04 1987-12-15 Mitsui Toatsu Chemicals, Incorporated Granule containing physiologically-active substance, method for preparing same and use thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2100951T3 (es) * 1990-12-31 1997-07-01 Pioneer Hi Bred Int Bacterias microencapsuladas en acido graso con disco giratorio, secadas.

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3959493A (en) * 1971-03-17 1976-05-25 Rumen Chemie, Ag Rumen bypass products comprising biologically active substances protected with aliphatic fatty acids
GB2016043A (en) * 1978-03-08 1979-09-19 Danochemo As Bacteria-containing product for use in animal feeds, and its production
US4675140A (en) * 1984-05-18 1987-06-23 Washington University Technology Associates Method for coating particles or liquid droplets
US4713245A (en) * 1984-06-04 1987-12-15 Mitsui Toatsu Chemicals, Incorporated Granule containing physiologically-active substance, method for preparing same and use thereof
US4710379A (en) * 1984-06-19 1987-12-01 Kabushiki Kaisya Advance Kaihatsu Kenkyujo Intestinal microflora-improving agent

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ATCC Catalogue of Bacteria & Bacteriophagese, 17th edition, issued 1989, page 79, see entire document. *
See also references of EP0604543A4 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0628072A1 (fr) * 1992-02-26 1994-12-14 Pioneer Hi-Bred International, Inc. Microspheres de micro-organismes sechees fabriquees selon un procede a disques rotatifs
EP0628072A4 (fr) * 1992-02-26 1995-12-20 Pioneer Hi Bred Int Microspheres de micro-organismes sechees fabriquees selon un procede a disques rotatifs.
EP0631616A1 (fr) * 1992-03-17 1995-01-04 Pioneer Hi-Bred International, Inc. Microspheres a base d'acide gras, contenant des anterocoques et dstinees a favoriser la croissance et a ameliorer la quantite de la carcasse
EP0631616A4 (fr) * 1992-03-17 1995-04-19 Pioneer Hi Bred Int Microspheres a base d'acide gras, contenant des anterocoques et dstinees a favoriser la croissance et a ameliorer la quantite de la carcasse.
WO1997045530A1 (fr) * 1996-05-27 1997-12-04 UZILOVA, Irina Semenovna, Heiress of UZILOV Utilisation de souches de streptococcus faecium et composition a base de ces souches
WO2003071883A1 (fr) 2002-02-28 2003-09-04 Centro Sperimentale Del Latte S.P.A. Compositions dietetiques et/ou pharmaceutiques destinees a l'humain et/ou l'animal, a base de preparations microbiennes probiotiques
WO2005009139A1 (fr) * 2003-07-30 2005-02-03 Chr. Hansen A/S Produit d'animaux d'elevage contenant une bacterie probiotique enterococcus

Also Published As

Publication number Publication date
MX9205329A (es) 1993-07-01
RO113477B1 (ro) 1998-07-30
JPH06511148A (ja) 1994-12-15
HU9400794D0 (en) 1994-06-28
BG98665A (bg) 1995-03-31
BG62175B1 (bg) 1999-04-30
CA2116525A1 (fr) 1993-04-01
EP0604543A1 (fr) 1994-07-06
CZ280601B6 (cs) 1996-03-13
CZ59594A3 (en) 1994-07-13
EP0604543A4 (en) 1994-07-27
SK32494A3 (en) 1994-08-10
RU2093571C1 (ru) 1997-10-20
SK278992B6 (sk) 1998-05-06
HUT67466A (en) 1995-04-28
BR9206505A (pt) 1995-04-25

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