WO1988006625A3 - Arginine-depleted human tumor necrosis factor - Google Patents

Arginine-depleted human tumor necrosis factor Download PDF

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Publication number
WO1988006625A3
WO1988006625A3 PCT/US1988/000183 US8800183W WO8806625A3 WO 1988006625 A3 WO1988006625 A3 WO 1988006625A3 US 8800183 W US8800183 W US 8800183W WO 8806625 A3 WO8806625 A3 WO 8806625A3
Authority
WO
WIPO (PCT)
Prior art keywords
arginine
necrosis factor
tumor necrosis
muteins
tnf
Prior art date
Application number
PCT/US1988/000183
Other languages
French (fr)
Other versions
WO1988006625A2 (en
Inventor
David F Mark
Leo S Lin
James W Thomson
Ralph Yamamoto
Original Assignee
Cetus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cetus Corp filed Critical Cetus Corp
Publication of WO1988006625A2 publication Critical patent/WO1988006625A2/en
Publication of WO1988006625A3 publication Critical patent/WO1988006625A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/525—Tumour necrosis factor [TNF]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Abstract

Muteins of tumor necrosis factor (TNF) which are arginine depleted are biologically active and have superior handling properties. Particularly preferred are deletions of, and substitutions by, neutral or acidic amino acids for the arginine residues at positions (2 and 6) of mature TNF. These muteins have higher homogeneity when subjected to isoelectric focusing. Deletion and substitution of arginine at positions (31 and 32) results in TNF muteins which are stable to the action of proteases.
PCT/US1988/000183 1987-02-26 1988-01-25 Arginine-depleted human tumor necrosis factor WO1988006625A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US1922187A 1987-02-26 1987-02-26
US019,221 1987-02-26

Publications (2)

Publication Number Publication Date
WO1988006625A2 WO1988006625A2 (en) 1988-09-07
WO1988006625A3 true WO1988006625A3 (en) 1988-10-20

Family

ID=21792071

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1988/000183 WO1988006625A2 (en) 1987-02-26 1988-01-25 Arginine-depleted human tumor necrosis factor

Country Status (2)

Country Link
AU (1) AU1346488A (en)
WO (1) WO1988006625A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3843534A1 (en) * 1988-12-23 1990-07-12 Basf Ag NEW TNF POLYPEPTIDES
FR2651130B1 (en) * 1989-08-23 1991-12-13 Roussel Uclaf SEQUENCE OF ANTI-SENSE OLIGONUCLEOTIDES, ANTI-RNA MESSAGE OF TNF ALPHA, METHOD OF PREPARATION, APPLICATION AS MEDICAMENTS AND PHARMACEUTICAL COMPOSITIONS.
EP0497922B1 (en) * 1989-10-24 2002-01-30 Chiron Corporation Infective protein delivery system
JPH05255393A (en) * 1990-09-21 1993-10-05 Ishihara Sangyo Kaisha Ltd Polypeptide
DE69121227T2 (en) * 1990-09-21 1997-02-20 Ishihara Sangyo Kaisha Polypeptide
CA2055168A1 (en) * 1990-11-21 1992-05-22 Walter Fiers Tnf-muteins
KR970005042B1 (en) * 1993-02-09 1997-04-11 한일합성섬유공업 주식회사 Tumor necrosis factor-alpha muteine
CA2119089A1 (en) * 1993-03-29 1994-09-30 David Banner Tumor necrosis factor muteins
IL120979A0 (en) * 1997-06-02 1997-11-20 Interpharm Lab Ltd Glycosylated TNF
GB0328261D0 (en) * 2003-12-05 2004-01-07 Univ Groningen Improved cytokine design
DK2953634T3 (en) 2013-02-07 2021-08-30 Massachusetts Gen Hospital Methods for expanding or depleting regulatory t cells
JP6925431B2 (en) 2016-11-09 2021-08-25 フィロジェン エッセ．ピー．アー． Immunoconjugates of IL2 and TNF mutants

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0155549A2 (en) * 1984-03-06 1985-09-25 Dainippon Pharmaceutical Co., Ltd. DNA encoding human tumor necrosis factor and human tumor necrosis factor polypeptide
EP0168214A2 (en) * 1984-07-05 1986-01-15 Genentech, Inc. Tumor necrosis factor, methods for its preparation, compositions containing it, DNA encoding it and assay method using such DNA
WO1986002381A1 (en) * 1984-10-15 1986-04-24 Cetus Corporation Human tumor necrosis factor
WO1986004606A1 (en) * 1985-02-07 1986-08-14 Cetus Corporation Cysteine-depleted muteins of biologically active human tumor necrosis factor proteins
EP0247906A2 (en) * 1986-02-04 1987-12-02 Mizuno, Den'Ichi DNA coding for anti-tumour polypeptides, the polypeptides and anti-tumour agents comprising said polypeptides

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0155549A2 (en) * 1984-03-06 1985-09-25 Dainippon Pharmaceutical Co., Ltd. DNA encoding human tumor necrosis factor and human tumor necrosis factor polypeptide
EP0168214A2 (en) * 1984-07-05 1986-01-15 Genentech, Inc. Tumor necrosis factor, methods for its preparation, compositions containing it, DNA encoding it and assay method using such DNA
WO1986002381A1 (en) * 1984-10-15 1986-04-24 Cetus Corporation Human tumor necrosis factor
WO1986004606A1 (en) * 1985-02-07 1986-08-14 Cetus Corporation Cysteine-depleted muteins of biologically active human tumor necrosis factor proteins
EP0247906A2 (en) * 1986-02-04 1987-12-02 Mizuno, Den'Ichi DNA coding for anti-tumour polypeptides, the polypeptides and anti-tumour agents comprising said polypeptides

Also Published As

Publication number Publication date
AU1346488A (en) 1988-09-26
WO1988006625A2 (en) 1988-09-07

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