WO1985000888A1 - A method and a device for the quantitative assay of nh3 in a sample - Google Patents
A method and a device for the quantitative assay of nh3 in a sample Download PDFInfo
- Publication number
- WO1985000888A1 WO1985000888A1 PCT/SE1984/000274 SE8400274W WO8500888A1 WO 1985000888 A1 WO1985000888 A1 WO 1985000888A1 SE 8400274 W SE8400274 W SE 8400274W WO 8500888 A1 WO8500888 A1 WO 8500888A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- sample
- sensing body
- polymer
- brought
- contact
- Prior art date
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
- G01N27/04—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance
- G01N27/12—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance of a solid body in dependence upon absorption of a fluid; of a solid body in dependence upon reaction with a fluid, for detecting components in the fluid
- G01N27/125—Composition of the body, e.g. the composition of its sensitive layer
- G01N27/126—Composition of the body, e.g. the composition of its sensitive layer comprising organic polymers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
Definitions
- a method and a device for the quantitative assay of NH 3 in a sample A method and a device for the quantitative assay of NH 3 in a sample.
- This invention relates to a method and a device for the assay of NH 3 in a sample. Primarily agricultural samples like manure and barn air etc. are concerned.
- the sample is alkalized and NH 3 is driven off, and captured in a vessel with acid.
- the ammonia content is determined by titration. This method is intended for laboratories only and demands skilled staff.
- a colour reaction with Nessler's reagent is utilized, the intensity of a yellow colour obtained being spectrophotometrically determined.
- the object of the present invention is to provide a method and a device for the quantitative assay of NH 3 in a sample, especially manure and the like, the demands just mentioned being met.
- such a method is characterized in that the sample is brought into contact with a sensing body, comprising a polymer, either based upon pyrrole or a derivative of such a polymer, in such a way, that the NH 3 of the sample is caused to act upon the sensing body, the resistance of same being determined before and after said action.
- figure 1 schematically shows a device according to the invention
- figure 2 shows the resistance of a sensing layer as a function of the time at different NH 3 concentrations.
- a carrier plate of non conductive material is given number 1.
- a thin layer of polypyrrole 2 in contact with two leads 3,4, which are connected to a resistance meter 5, which is not further described.
- a resistance meter 5 On the back side of the carrier plate there is glued a little circuit of resistor wire, which is not shown in the figure.
- This resistor wire is connected to a low voltage source.
- the carrier plate is fastened to a plug 6, which fits a cover to a test tube 7. In the figure this test tube is shown filled to about 1/3 with a sample 8.
- FIG 2 there is shown the result of measurements of three test solutions of water, containing 500-1000-1500 ppm NH 3 , and a manure sample.
- a 4 ml sample was introduced into the test tube, and 0.1 ml 10 M NaOH was added, whereupon the measuring device was inserted quickly into the test tube 7, which was then closed by the plug 6.
- NaOH NH 4 + and NH 3 in the sample were transferred to the gas phase.
- the resistance was determined continuously during 10 minutes and the curves in figure 2 were drawn. With the aid of the calibration curves it can be established that the NH 3 -concentration in the manure sample Is about 750 ppm.
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Investigating Or Analyzing Materials By The Use Of Fluid Adsorption Or Reactions (AREA)
- Laminated Bodies (AREA)
- Reinforced Plastic Materials (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The NH3-concentration in a sample is determined by the aid of a device, comprising a layer of a polymer, based upon pyrrole or a derivative of pyrrole, or a derivative of such a polymer. The layer is acted upon by NH3, originating from the sample, and its resistance is measured before and after this action. Increasing concentrations of NH3 mean increasing resistance in said layer.
Description
A method and a device for the quantitative assay of NH3 in a sample.
This invention relates to a method and a device for the assay of NH3 in a sample. Primarily agricultural samples like manure and barn air etc. are concerned.
A number of more or less sophisticated methods for the quantitative assay of NH3 in different types of samples have been suggested and applied. Thus there are electrodes for the determination of NH3/NH4 +. By adding alkali the equilibrium is displaced towards NH3. The method is primarily intended for laboratories and demands relatively advanced instrumental equipment and a sensitive electrode. Thus the method is relatively expensive.
According to another laboratory method the sample is alkalized and NH3 is driven off, and captured in a vessel with acid. The ammonia content is determined by titration. This method is intended for laboratories only and demands skilled staff.
According to one further laboratory method a colour reaction with Nessler's reagent is utilized, the intensity of a yellow colour obtained being spectrophotometrically determined.
Straight chemical methods are also employed, e.g. by oxidizing NH3 to N2 and measuring the change of volume or pressure obtained.
None of these methods meets the demands of being simple, reliable, inexpensive and giving sufficient accuracy of measurement. These demands are actual in many situations, for instance among farmers for samples of manure, barn air etc.
The object of the present invention is to provide a method and a
device for the quantitative assay of NH3 in a sample, especially manure and the like, the demands just mentioned being met.
According to the invention such a method is characterized in that the sample is brought into contact with a sensing body, comprising a polymer, either based upon pyrrole or a derivative of such a polymer, in such a way, that the NH3 of the sample is caused to act upon the sensing body, the resistance of same being determined before and after said action.
It has namely become obvious that the resistance of a polymer layer of the type just mentioned increases with rising NH3concentration in a sample that is brought into contact with said layer. Either the sample can be brought into direct contact with the layer, or gas phase, originating from the sample can be brought into contact with the layer. As liquid water disturbs the measurement it may be suitable to provide a membrane that is permeable for a gas but not for a liquid, between the sample and said layer. This membrane may be somewhat spaced from the layer or be applied in direct contact with the polymer layer.
Considering the fact that a liquid like water disturbs the measurement it can be suitable to maintain the layer at a some what higher temperature than the sample, e.g. 10° higher, so that condensate is avoided.
The invention shall now be described more in detail, reference being made to the accompanying figures, in which
figure 1 schematically shows a device according to the invention, and
figure 2 shows the resistance of a sensing layer as a function of the time at different NH3 concentrations.
In figure 1 a carrier plate of non conductive material is given number 1. On same there is applied a thin layer of polypyrrole 2, in contact with two leads 3,4, which are connected to a resistance meter 5, which is not further described. On the back side of the carrier plate there is glued a little circuit of resistor wire, which is not shown in the figure. This resistor wire is connected to a low voltage source. The carrier plate is fastened to a plug 6, which fits a cover to a test tube 7. In the figure this test tube is shown filled to about 1/3 with a sample 8.
In figure 2 there is shown the result of measurements of three test solutions of water, containing 500-1000-1500 ppm NH3, and a manure sample. When measuring, at a temperature of 20° in the samples and 30°C in the polymer layer, a 4 ml sample was introduced into the test tube, and 0.1 ml 10 M NaOH was added, whereupon the measuring device was inserted quickly into the test tube 7, which was then closed by the plug 6. By the addition of NaOH NH4 + and NH3 in the sample were transferred to the gas phase. The resistance was determined continuously during 10 minutes and the curves in figure 2 were drawn. With the aid of the calibration curves it can be established that the NH3-concentration in the manure sample Is about 750 ppm.
Claims
1. A method for the quantitative assay of NH3 in a sample cha r a c t e r i z e d i n that the sample is brought into contact with a sensing body, comprising a polymer, either based upon pyrrole or a derivative of pyrrole, or a derivative of such a polymer, so that the NH3 of the sample is caused to act upon the sensing body, the resistance of same being determined before and after said action.
2. A method according to claim 1, c h a r a c t e r i z ed i n that the sample is brought into direct contact with said sensing body.
3. A method according to claim 1, ch ar a c t e r i z e d i n that the sample is brought Into contact with the sensing body.
4. A method according to claim 3, c ha r a c t e r i z e d i n that said gas phase is brought Into contact with the sensing body after having passed a membrane, which is permeable for a gas but not for a liquid.
5. A method according to any of claims 1-4, c h a r a ct e r i z e d i n that the sensing body is heated to a temperature somewhat higher than the temperature of the sample in order to avoid condensate on same when measuring.
6. A device for carrying out the method according to any of claims 1-5, c ha r a c t e r i z e d by a sensing body, comprising a polymer, either based upon pyrrole or a derivative of pyrrole, or a derivative of such a polymer, connected by leads to a resistance meter.
7. A device according to claim 6, c h a r a c t e r i z e d i n that the sensing body comprises a carrier, onto which there is applied a relatively thin layer of said polymer.
8. A device according to claim 6, in which gas phase, originating from the sample, is brought into contact with the sensing body, c h a r a c t e r i z e d i n that a membrane, permeable for a gas, but not for a liquid, is provided between the sample and the sensing body.
9. A device according to claim 8, c h a r a c t e r i z e d i n that the membrane is made from polytetrafluorethylene.
10. A device according to any of claims 6-9, c h a r a ct e r i z e d i n that the sensing body is provided with a circuit for electrical heating.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DK170885A DK170885D0 (en) | 1983-08-17 | 1985-04-16 | METHOD AND APPARATUS FOR QUANTITATIVE DETERMINATION OF NH3 IN A TEST |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE8304474A SE446776B (en) | 1983-08-17 | 1983-08-17 | PROCEDURE FOR QUANTITATIVE SEATING OF NH? 713 IN A SAMPLE AND DEVICE FOR CARRYING OUT THE PROCEDURE |
SE8304474.3 | 1983-08-17 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1985000888A1 true WO1985000888A1 (en) | 1985-02-28 |
Family
ID=20352226
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/SE1984/000274 WO1985000888A1 (en) | 1983-08-17 | 1984-08-10 | A method and a device for the quantitative assay of nh3 in a sample |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP0183697A1 (en) |
AU (1) | AU3311384A (en) |
DK (1) | DK170885D0 (en) |
SE (1) | SE446776B (en) |
WO (1) | WO1985000888A1 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0398286A2 (en) * | 1989-05-18 | 1990-11-22 | Nisshinbo Industries, Inc. | Ammonia sensor |
GB2364778A (en) * | 2000-07-14 | 2002-02-06 | Hypoguard Ltd | Detection of Helicobacter pylori and apparatus therefor |
US6509169B2 (en) | 2000-07-14 | 2003-01-21 | University Of West England, Bristol | Detection of Helicobacter pylori |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2077437A (en) * | 1980-06-07 | 1981-12-16 | Emi Ltd | Ammonia gas sensors |
-
1983
- 1983-08-17 SE SE8304474A patent/SE446776B/en not_active IP Right Cessation
-
1984
- 1984-08-10 EP EP19840903098 patent/EP0183697A1/en not_active Withdrawn
- 1984-08-10 AU AU33113/84A patent/AU3311384A/en not_active Abandoned
- 1984-08-10 WO PCT/SE1984/000274 patent/WO1985000888A1/en unknown
-
1985
- 1985-04-16 DK DK170885A patent/DK170885D0/en active IP Right Grant
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2077437A (en) * | 1980-06-07 | 1981-12-16 | Emi Ltd | Ammonia gas sensors |
Non-Patent Citations (1)
Title |
---|
Chemical Abstracts, Vol 100 (1984) abstract nr 144174, Anal. Chem. Symp ser. 1983 17 (chem sens.) 203-7 (Eng), 30 April 1984 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0398286A2 (en) * | 1989-05-18 | 1990-11-22 | Nisshinbo Industries, Inc. | Ammonia sensor |
EP0398286A3 (en) * | 1989-05-18 | 1991-09-25 | Nisshinbo Industries, Inc. | Ammonia sensor |
GB2364778A (en) * | 2000-07-14 | 2002-02-06 | Hypoguard Ltd | Detection of Helicobacter pylori and apparatus therefor |
US6509169B2 (en) | 2000-07-14 | 2003-01-21 | University Of West England, Bristol | Detection of Helicobacter pylori |
Also Published As
Publication number | Publication date |
---|---|
DK170885A (en) | 1985-04-16 |
SE8304474L (en) | 1985-02-18 |
DK170885D0 (en) | 1985-04-16 |
EP0183697A1 (en) | 1986-06-11 |
AU3311384A (en) | 1985-03-12 |
SE8304474D0 (en) | 1983-08-17 |
SE446776B (en) | 1986-10-06 |
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