US3576800A - 1-acyl-3-indolyl aliphatic acid derivatives and their method of preparation - Google Patents

1-acyl-3-indolyl aliphatic acid derivatives and their method of preparation Download PDF

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US3576800A
US3576800A US635362A US3576800DA US3576800A US 3576800 A US3576800 A US 3576800A US 635362 A US635362 A US 635362A US 3576800D A US3576800D A US 3576800DA US 3576800 A US3576800 A US 3576800A
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acid
methyl
methoxyphenyl
methoxy
derivative
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Hisao Yamamoto
Masaru Nakao
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Sumitomo Chemical Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/40—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil
    • A61K31/403—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil condensed with carbocyclic rings, e.g. carbazole
    • A61K31/404—Indoles, e.g. pindolol
    • A61K31/405—Indole-alkanecarboxylic acids; Derivatives thereof, e.g. tryptophan, indomethacin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C243/00—Compounds containing chains of nitrogen atoms singly-bound to each other, e.g. hydrazines, triazanes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C251/00—Compounds containing nitrogen atoms doubly-bound to a carbon skeleton
    • C07C251/72—Hydrazones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/04—Indoles; Hydrogenated indoles
    • C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
    • C07D209/18—Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/04—Indoles; Hydrogenated indoles
    • C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
    • C07D209/18—Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
    • C07D209/26—Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals with an acyl radical attached to the ring nitrogen atom
    • C—CHEMISTRY; METALLURGY
    • C30—CRYSTAL GROWTH
    • C30B—SINGLE-CRYSTAL GROWTH; UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL OR UNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL; REFINING BY ZONE-MELTING OF MATERIAL; PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; APPARATUS THEREFOR
    • C30B19/00—Liquid-phase epitaxial-layer growth
    • C30B19/02—Liquid-phase epitaxial-layer growth using molten solvents, e.g. flux
    • C30B19/04—Liquid-phase epitaxial-layer growth using molten solvents, e.g. flux the solvent being a component of the crystal composition
    • C—CHEMISTRY; METALLURGY
    • C30—CRYSTAL GROWTH
    • C30B—SINGLE-CRYSTAL GROWTH; UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL OR UNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL; REFINING BY ZONE-MELTING OF MATERIAL; PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; APPARATUS THEREFOR
    • C30B19/00—Liquid-phase epitaxial-layer growth
    • C30B19/06—Reaction chambers; Boats for supporting the melt; Substrate holders
    • C30B19/061—Tipping system, e.g. by rotation
    • C—CHEMISTRY; METALLURGY
    • C30—CRYSTAL GROWTH
    • C30B—SINGLE-CRYSTAL GROWTH; UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL OR UNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL; REFINING BY ZONE-MELTING OF MATERIAL; PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; APPARATUS THEREFOR
    • C30B29/00—Single crystals or homogeneous polycrystalline material with defined structure characterised by the material or by their shape
    • C30B29/10—Inorganic compounds or compositions
    • C30B29/40—AIIIBV compounds wherein A is B, Al, Ga, In or Tl and B is N, P, As, Sb or Bi
    • C30B29/42—Gallium arsenide

Definitions

  • a 1-acyl-3-indolyl aliphatic acid derivative having re- P e f e found that hVe1 l y y markable anti-inflammatory, antipyretic and analgesic ahlfhauc jacld derlvatlves havlhg h hhh'lhflemmatory, actions which is characterized in that said acyl substituent antl'pyretlc and analgeslc actlvltles were P p y is aralkylcarbonyl or aralkenylcarbonyl whose carbon novel or known proeesseschain has up to 5 carbon atoms, and process for producone hl of e P e h on Is to prov de novel i the compound 1-acyl-3-1ndolyl aliphatic acid der1vat1ves having high anti-inflammatory, anti-pyretic and analgesic activities and processes
  • Another BACKGROUND OF THE INVENTION object of the present invention is to provide processes for economically manufacturing these compounds in a Fleld of the lhvehhoh high yield.
  • a further object of the present invention is The present invention relates to novel N-substituted to Provide a novel Pharmaceutical f pe containing indole derivatives having high anti-inflammatory, antithese compounds as the efiectlve lhgfedleht- Still f t pyretic and analgesic activities and to processes for proohleets W111 he -PP h from e fOnOWlhg desenlehohducing the same.
  • the present invention In Order to aeeomphsh these l the P e Y relates to novel 1-acyl-3-indolyl aliphatic acid derivatives h Preludes hovel y ahPhahe field derlvaand processes for producing the same. fives 0f the formula? (2) Prior art 40 1 (1'1 Of the developed non-steroidal anti-inflammatory com- 4 3 CH m (CH2)n O p 0 pounds, 1-(p-chlorobenzoyl)-2-methyl-5-meth0xy-3indo- 5 lylacetic acid is greatest in activity. But it is high in toX- R-- icity. The present inventors also observed that even when 6 1 5 l0 mg./kg.
  • R is an unsubstituted or a lower alkyl-, lower 1,2 diphenyl 3,5 dioxo 4 n butylpyrazolidine alkoXy-, lower alkylthio-, nitro-, cyanoor halogen- (phenylbutazone), which is most widely used as antisubstituted aromatic ring group, each of said alkyl, alkoxy phlogistic at present, has low activity in comparison to and alkylthio substituents containing up to 4 carbon its high acute toxicity and hence is considerably small atoms, or an unsubstituted or a methyl-, ethylor halogenin therapeutic ratio.
  • R each are hydrogen atoms or alkyl groups having up Heterocyclic Compounds, vol. 3 (1952), chapter 1, to 3 carbon atoms; R is a hydrogen atom, a carboxy pages 1-247, and W.
  • l-Substituted acyl groups atoms, a benzyloxy group, a tetrahydropyranyloxy group, of l-acyl-indole derivatives are so easily hydrolyzed by an amino group or a hydroxy group;
  • R is an alkyl group acid or alkali that it has been considered impossible to having up to 4 carbon atoms, an alkoxy group having up obtain l-acyl-indole derivatives directly from correspond to 4 carbon atoms, an alkylthio group having up to 4 ing N -acylated phenylhydrazine derivatives by Fischers carbon atoms, a nitro group, an alkenyl group having indolization.
  • Suvorov et a1. Doklady up to 4 carbon atoms, an alkenyloxy group having up Acad. Nauk S.S.S.R. 136, 840 (1961), Chem. Abstn, 55, to 4 carbon atoms, a halogen atom or a hydrogen atom;
  • A is an unsubstituted saturated hydrocarbon chain having up to 5 carbon atoms, an unsubstituted unsaturated hydrocarbon chain having up to 5 carbon atoms, a halogen-substituted saturated hydrocarbon chain having up to 5 carbon atoms, a halogen-substituted unsaturated hydrocarbon chain having up to 5 carbon atoms, a phenylsubstituted saturated hydrocarbon chain having up to 5 carbon atoms or a phenyl-substituted unsaturated hydrocarbon chain having up to 5 carbon atoms, the hydrocarbon chain being a straight one or a branched one; m and p each are or 1; and n is 0 or an integer of from 1 to 3.
  • the present invention provides a process for producing novel l-acyl-S-indolyl aliphatic acid derivatives represented by the Formula 1, which comprises reacting an N -acylated phenylhydrazine derivative of the formula:
  • R R R R m, n and p have the same meanings as identified above.
  • the present invention provides a process for producing novel 1-acyl-3-indolyl aliphatic acid derivatives represented by the Formula I, which comprises decomposing an N -acylated phenylhydrazone derivative of the formula:
  • R and A have the same meanings as identified above and Y represents a halogen or an ester residue, to yield an N -acylated phenylhydrazone derivative represented by the Formula IV, decomposing the resultant N -acylated phenylhydrazone derivative with a decomposing agent to give an N -acylated phenylhydrazine derivative represented by the Formula II, and reacting the resultant N -acylated phenylhydrazine derivative with an aliphatic acid derivative represented by the Formula III to yield the 1-acy1-3-indolyl aliphatic acid derivative (I).
  • the present invention provides a process for producing novel l-acyl-3-indolyl aliphatic acid derivatives of the Formula I; which comprises reacting a phenylhydrazone derivative of the Formula V with a compound having the Formula VI to yield an N -acylated phenylhydrazine derivative of the Formula II, and reacting the resultant N -acylated phenylhydrazine derivative of the Formula II with an aliphatic acid compound of the Formula III to yield an 1-acy1-3-indolyl aliphatic acid derivative (1).
  • the present invention provides a process for producing novel 1-acyl-3-indolyl aliphatic acid derivatives of the Formula I, which comprises reacting an N -acylated phenylhydrazone derivative of the Formula IV with an aliphatic acid derivative of the Formula III to yield the l-acyl-S-indolyl aliphatic acid derivative of the Formula I.
  • the present invention provides a process for producing novel l-acyl-3-indolyl aliphatic acid derivatives of the Formula I, which comprises reacting a phenylhydrazone derivative of the Formula V with a compound having the Formula VI to yield an N -acylated phenylhydrazone derivative of the Formula IV and reacting the resultant N -acylated phenylhydrazone derivative (IV) with an aliphatic acid derivative of, the Formula III to yield the l-acyl-3-indolyl aliphatic acid derivative (I).
  • the present invention provides a process for producing novel 1-acyl-3-indoly1 aliphatic acid derivatives of the Formula VII:
  • the present invention provides a process for producing novel l-acyl-3-indolyl aliphatic acid derivatives of the Formula VII, which comprises reacting an N -acylated phenylhydrazone derivative of the Formula IV with a compound of the Formula VIII to yield the lacyl-3-indoly1 aliphatic acid derivative of the Formula VII.
  • the present invention provides a process for producing novel 1-acyl3-indolylacetic acid derivatives wherein R R and A have the same meanings as identified above, which comprises reacting an N -acylated phenylhydrazine derivative of the Formula II with acetosuccinic acid or 2-ketoadipic acid to yield the l-acyl-3-indolylacetic acid derivative of the Formula IX.
  • the present invention provides a process for producing novel 1-acyl-3-indolylacetic acid derivatives of the Formula IX, which comprises reacting an N acylated phenylhydrazone derivative of the Formula IV with acetosuccinic acid or 2-ketoadipic acid to yield the 1-acyl-3-indoly1acetic acid (IX).
  • the present invention provides a process for producing novel l-acyl-3-indolyl aliphatic acid derivatives of the Formula I, which comprises reacting a phenylhydrazine derivative of the formula:
  • R has the same meaning as identified above, with a compound having the Formula VI to yield an N acylated phenylhydrazine derivative of the Formula II, and reacting the resultant N -acylated phenylhydrazine derivative (II) with an aliphatic acid compound of the Formula III to yield the 1-acyl-3-indolyl aliphatic acid derivative (I).
  • the present invention provides a process for producing novel 1-acyl-3-indolyl aliphatic acid derivatives of the Formula I, which comprises reacting a phenylhydrazine derivative of the Formula XI:
  • R R and A have the same meaning as identified in the Formula I, with an aliphatic acid derivative (III).
  • the present invention provides a process for preparing novel l-acyl-3-indolyl aliphatic acid derivatives represented by the formula:
  • the present invention provides a process for producing novel 1-acyl-3-indoly1 aliphatic acid derivatives of the Formula XII, which comprises reacting an N -acylated phenylhydrazine derivative of the Formula II with a compound of the formula:
  • R R R R and A have the same meanings as identified above, which comprises oxidizing an indole-3- aliphatic aldehyde derivative represented by the formula:
  • the present invention provides a process for producing novel 1-acyl-3-indolyl aliphatic acid derivatives of the Formula XV, which comprises reacting an N acylated phenylhydrazine derivative of the Formula II with a compound of the formula:
  • R and R have the same meanings as identified above and R is a lower alkyl group, to yield a 3-indolyl aliphatic aldehyde acetal derivative of the formula:
  • the present invention provides a process for preparing novel 1-acyl-3-indolyl aliphatic acid derivatives of the formula:
  • the present invention provides a process for producing novel 1-acyl-3-indolyl aliphatic acid derivatives of the Formula XV which comprises reacting an N -acylated phenylhydrazine derivative of the Formula II with a compound of the formula:
  • the present invention provides a process for preparing novel l-acyl-3-indolyl aliphatic acid derivatives of the Formula XV which comprises dehydrogenating a 2,3-dihydro-3-indolyl aliphatic acid derivative of the formula:
  • the present invention provides a process for preparing novel l-acyl-3-indolylacetic acid derivatives of the formula:
  • R R R R and A have the same meanings as identified above, which comprises dehydrating or dehydrating and hydrolyzing thereafter a 2-hydro-3-hydroxy-3- indolylacetic acid derivatives of the formula:
  • the present invention provides a process for preparing novel l-acyl-3-indolyl aliphatic acid derivatives of the Formula XV which comprises subjecting a(N- acylanilino)-aliphatic acid derivatives of the formula:
  • the present invention provides a process for preparing novel 1-acyl-3-indolylacetic acid derivatives of the Formula IX, which comprises heating a 3-(2'- acylaminophenyl) -levulinic acid derivative of the formula:
  • a 1-acyl-3-indoly1 aliphatic acid derivative represented by the Formula I is prepared by the reactions shown by the following equations:
  • N -acylated phenylhydrazine derivatives (II) and N -acylated phenylhydrazone derivatives (IV) may be synthesized as intermediates by other processes than those disclosed herein. These intermediates, the compounds (II) and (IV), are novel compounds.
  • the reaction of a phenylhydrazone derivative (V) with a. compound (VI) is carried out in the presence of a hydrogen halide acceptor.
  • a hydrogen halide acceptor a tertiary amine, for example, pyridine or dimethylaniline can be used.
  • These hydrogen halide acceptors themselves can be used as solvents.
  • Inert solvents such as ether, benzene, toluene, and tetrahydrofuran are also able to be used as reaction solvents in the presence of equimolar or larger amounts of these hydrogen halide acceptors.
  • the compound (VI) may be chloride, bromide, iodide or fluoride and chloride is most preferable from a commercial point of view.
  • the reaction proceeds at room temperature in many cases, and even below 0 C. in some kind of solvent used.
  • the produced hydrogen halide salt of the hydrogen halide acceptor is filtered off and the filtrate is concentrated under a reduced pressure, or the reaction mixture is poured into water when a water-soluble solvent like pyridine is used as the solvent.
  • the aimed N -acylated phenylhydrazone compound is easily obtained as crystals or an oily substance.
  • These products can be purified with an appropriate solvent, for example, the solvent mixture of alcohol and water.
  • N -acylated phenylhydrazine derivative (II) is directly obtained in place of an N -acylated phenylhydrazone derivative (IV).
  • N -acylated phenylhydrazone compounds (IV) there are illustrated:
  • N -cinnamoyl-N (p-methoxyphenyl hydrazine N -cinnamoy1-N -(p-tolyl)hydrazine N -cinnamoyl-N -(m-methoxyphenyl)hydrazine N -(m-nitrocinnamoy1) -N -(p-methoxyphenyl) hydrazine 1 2 N phenylacetyl) -N p-methoxyphenyl) hydrazine N (p-tolylacetyl) -N (p-methoxyphenyl) hydrazine N 2'-furylacetyl N (p-rnethoxyphenyl) hydrazine Those novel N -acylated phenylhydrazone derivatives and N -acylated phenylhydrazine derivatives which are obtained by the method of the present invention have psychic, stimulating, anti-tumor, bactericid
  • N -acylated phenylhydrazone derivative (IV) is dissolved or suspended in an adequate solvent, for example, alcohol, ether, benzene or toluene.
  • an adequate solvent for example, alcohol, ether, benzene or toluene.
  • alcohol absolute alcohol achieves the good yield.
  • more than equivalent of dry hydrogen chloride gas is absorbed into the resultant solution or the suspension.
  • the HCl salt of the N -acylated phenyl-hydrazine derivative (II) precipitates as crystals in good yield.
  • Sulfuric acid or others can be used in place of gaseous hydrogen chloride.
  • ether, benzene or toluene is used as the solvent, a small quantity of alcohol should be added to it.
  • the reaction temperature is preferably 0-25 C. though may be below 0 C.
  • N -acylated phenylhydrazone derivative (IV) various compounds can be illustrated.
  • the hydrazones of acetaldehye, chloral, benzaldehyde, acetal, ethyl acetoacetate and methoxy acetone can be easily decomposed in general cases to give the aimed N -acylated phenylhydrazine derivative (II).
  • the hydrazone of acetaldehyde has especially distinctive commercial advantages.
  • N acylated phenylhydrazine derivatives (II), for example, can be obtained:
  • N -cinnamoyl-N (p-methoxyphenyl) hydrazine N -cinnamoyl-N p-methylphenyl )hydrazine N -cinnamoyl-N (p-chlorophenyl) hydrazine N -cinnamoy1-N -(p-methylthiophenyl hydrazine N -cinnamoy1-N (p-ethoxyphenyl hydrazine N 5-phenyl-2,4-pentadienoyl) -N (:p-rnethoxyphenyl) hydrazine N (5 -phenyl-2',4'-p ent adienoyl) -N (p-ethoxyphenyl) hydrazine N -cinnamoyl-N (p-methoxyphenyl) hydrazine N -cinnarnoyl-N (p-methylphenyl
  • novel N -acylated phenylhydrazine derivatives (II) are directly obtained by reacting a phenylhydrazine derivative (X),
  • This reaction is carried out in a conventional solvent such as benzene, toluene, xylene, ether, dioxane or tetrahydrofuran in the presence of a dehydrogenhalide agent such as a tertiary amine.
  • a dehydrogenhalide agent such as a tertiary amine.
  • a tertiary amine triethyl amine, pyridine or dimethylaniline is suitable and the amount thereof is required preferably at least equal mole of the said phenylhydrazine derivative (X).
  • R phenyl, p chlorophenyl, p methylphenyl, p-methoxyphe-nyl, p-bromophenyl, p-ethylphenyl, p-ethoxy-- phenyl, 3-pyridyl, 4-pyridyl, Z-furyl, 3-furyl, Z-thienyl, S-thienyl, 5-chloro-2-thienyl, N-methyl-B-pyperidyl, anaphthyl, hydrogen atom;
  • R chlorine, bromine, fluorine, hydrogen, methyl, ethyl, isopropyl, methoxy, ethoxy, propioxy, methylthio, ethylthio and isopropylthio.
  • the reaction is carried out on heating in the presence of an adequate condensing agent or not in an organic solvent or not.
  • the yield is very high.
  • This reaction is carried out on heating in the presence solvent but it is preferable to use a suitable solvent in many cases.
  • organic acids for example acetic acid, formic acid, propionic acid, lactic acid, butyric acid, non-polar organic solvents, for example cyclohexane, n-hexane, benzene, toluene, and other organic solvents, such as dioxane and dimethyl formamide are used in the ring formation reaction.
  • organic acids for example acetic acid, formic acid, propionic acid, lactic acid, butyric acid
  • non-polar organic solvents for example cyclohexane, n-hexane, benzene, toluene, and other organic solvents, such as dioxane and dimethyl formamide
  • a corresponding ester of indole aliphatic acid is produced.
  • the reaction generally proceeds but a temperature within a range of 65 to 95 C. is preferable.
  • the reaction proceeds rapidly and is generally finished in a short period of reaction time, mostly in one or two hours.
  • the condensing agent is not needed in some cases but desirable results are generally achieved by using a condensing agent.
  • the condensing agent includes inorganic acids such as hydrochloric acid, sulfuric acid and phosphoric acid, metal halides such as zinc chloride and copper chloride, heavy metal powder such as copper powder, Grignards reagents, boron fluorides, polyphosphoric acid or ion-exchange resins. Hydrochloric acid or the like is required in an equimolar or larger amount, while copper powder or the like may be in a small amount,
  • the reaction mixture is allowed to stand at room temperature or in a refrigerator (about 5 C.), and then a large amount of crystals of the product is mostly obtained.
  • reaction solvents, reaction conditions, condensing agents and recrystallization solvents which have been mentioned above are only presented as illustrative of the present invention but not in its limitation, needless to speak of.
  • 01120 O OH 22 wherein R R and A have the same meanings as identitied in the Formula I, is prepared from an N -acyl phenylhydrazine derivative (II) or salt thereof and acetosuccinic acid or 2-ketoadipic acid.
  • a 1-acyl-3-indolyl aliphatic acid derivative (1) can be obtained directly from an N -acylated hydrazone derivative (IV) in some cases, in which a 1-acyl-3-
  • organic acids such as formic acid, propionic acid, lactic acid and butyric acid
  • non-polar organic solvents such as cyclohexane, nhexane, benzene and toluene or an alcohol
  • the reaction generally proceeds at 50 to 200 C., preferably at 65 to C.
  • metal halides such as Zinc chloride and copper chloride, boron fluoride and polyphosphoric acid can be used as a condensing agent in the reaction.
  • a 1- acyl-3-indolyl aliphatic acid derivative represented by the Formula I is prepared from the phenylhydrazine derivative of the Formula IX or a salt thereof and an aliphatic acid derivative of the Formula III in the presence or absence of a suitable solvent, and in the presence or absence of a condensing agent.
  • suitable solvents are organic acids, such as acetic acid, formic acid, propionic acid and lactic acid, non-polar solvents such as cyclohexane, n-hexane, benzene, toluene and xylene, ether compounds such as dioxane and diisopropyl ether or other conventional organic solvents.
  • Suitable condensing agents are inorganic acids such as hydrochloric acid, sulfuric acid, phosphoric acid and hydrogen chloride, metal halides such as zinc chloride, copper chloride, a metal powder, such as copper powder, a Grignards reagent, a boron fluoride, polyphosphoric acid or an ion-exchanger resin.
  • This 'indole ring formation proceeds under heating the reaction mixture at 50 to 200 C., and is completed within several hours. Preferably, the reaction is continued at 65 to 95 C. for 1 to 4 hours.
  • the reaction mixture After completion of the reaction, the reaction mixture is allowed to cool, a large amount of crystals are generally produced as precipitate. Even in the case which crystals are not produced, if the solvent is removed by dis- 70 tillation or water or petroleum ether is added to the reaction mixture, a large amount of crystals can be obtained.
  • the crude crystals are collected by filtration, washed with water and thereafter recrystallized from a suitable solvent to give a pure objective product.
  • l-acyl-3-indolyl aliphatic acid derivatives which are prepared by this method are as follows, but it is not necessary to say that 1-acyl-3-indolyl aliphatic acid derivatives are not restricted only to them.
  • a 1-acyl-3-indolyl aliphatic acid derivative (XII) is obtained by converting an ester or amide derivative (XIII) of a corresponding 3-indo1yl aliphatic acid.
  • a benzyl ester of a 3-indolyl aliphatic acid is converted to a free 3-indoyl aliphatic acid derivative (XII) by hydrogenating with decomposition in the presence of a metal catalyst such as palladium.
  • ester compound (X) is tertiary butyl alcohol
  • the ester is treated with an arylsulfonic acid such as p-toluenesulfonic acid to yield an objective product.
  • a free 3-indolyl aliphatic acid derivative may be obtained by treating an amide of the corresponding 3-indo1yl aliphatic acid derivative with a suitable amount of nitrous acid in an inert solvent.
  • potassium permanganate, hydrogen peroxide, organic peroxide, silveroxide, selenium dioxide and copper hydroxide can be used as an oxidizing agent, and especially potassium permanganate or copper hydroxide is most preferable in many cases.
  • R and R hydrogen, methyl and ethyl.
  • propyl isopropyl, tertiary-butyl, methylthio, ethylthio and hydrogen.
  • a 1- acyl-3-indolylacetic acid derivative of the Formula XV is prepared by oxidizing the corresponding indole-3-ethanol derivative of the Formula XIX.
  • an indole-3-ethanol derivative(XIX) is oxidized by potassium permanganate or chromic acid to give a 1-acyl-3-indolylacetic acid derivative (XV) in a good yield.
  • 2- 3 '-pyridy1)-vinyl 2-(2-furanyl) -viny1, p-methoxybenzyl, p-methylbenzyl, 2,2-diphenylvinyl, Z-(cinnamyl)-vinyl, N-methylhexahydro-3-pyridylmethyl, 2-(4'- pyridyl)-viny1 and 2-(5 '-chloro-2-thieny1)-vinyl.
  • propyl iso-propyl, tertiary-butyl, methylthio, ethylthio and hydrogen.
  • R R R R and A have the same meanings as identified above.
  • a l-acyl 3 indolylacetic acid derivative (XV) is prepared by dehydrogenation of the corresponding 2,3-dihydro-3-indolylacetic acid derivative (XXI).
  • non-polar solvent such as benzene, xylene and toluene and other various organic solvents, such as acetone, acetic acid, chloroform, ethanol and methanol
  • organic solvents such as acetone, acetic acid, chloroform, ethanol and methanol
  • chloranil, selenium dioxide, halogen and the like oxidizing agents can be used as agents for the dehydrogenation.
  • a 1- acyl-3-indolylacetic acid derivative of the Formula XXII is prepared by dehydration of a Z-hydro 3 hydroxy- 3-indolylacetic acid derivative of the Formula XXIII and then, if necessary, by hydrolysis of the rwultant ester compound.
  • the reaction proceeds at a temperature within the range of 70 to 200 C.
  • the compound (XXIII) is azeotropically refluxed with an azeotropic solvent, for example benzene, toluene or xylene, or is heated in the presence of a suitable dehydrating agent, for example a proper amount of anhydrous sodium sulfate, whereby a dehydration reaction takes place.
  • an azeotropic solvent for example benzene, toluene or xylene
  • a suitable dehydrating agent for example a proper amount of anhydrous sodium sulfate
  • the 2-hydro 3 hydroxy 3 indolylacetic acid derivative :(XXIII), the starting compound of the above process, is prepared by heating the mixture of alkyl halogenoacetate and a corresponding indole derivative with stirring in a non-polar organic solvent in the presence of zinc powder and, if necessary, a small piece of iodine.
  • One example of the above reaction is as follows:
  • a crude l-acyl 3 indolylacetic acid derivative (XV) is obtained by heating an 'y-(N-acyl-anilino)-aliphatic acid derivative (XXIV) in the presence of a suitable catalyst or a dehydrating agent.
  • the crude product thus obtained can be purified by recrystallization from a proper organic solvent or, if the recrystallization fails, a columnchromatography can be used.
  • R and R hydrogen, methyl and ethyl.
  • n-propyl iso-propyl, tertiary butyl, methylthio, ethylthio and hydrogen.
  • the y (N acyl-anilino)-aliphatic acid derivative (XXIV), which is a starting substance of this method, is generally prepared by following processes:
  • a 1-acyl-3-indolylacetic acid compound (IX) is prepared from a 3-(2'-acylaminophenyl)-levulinic acid derivative represented by the Formula XXV in the presence of inorganic acid in a suitable solvent.
  • a range of reaction temperatures is from 40 to 120 C., and the most desirable result is obtained at temperature within the range of 60 to C.
  • reaction mixture is neutralized, concentrated, and extracted with a suitable organic solvent, and then a separated organic layer is dried and concentrated to a crude crystalline substance.
  • novel 3-indolyl aliphatic acid derivatives of the present invention there are not only a few useful compounds, which indicate excellent anti-inflammatory action but also possess extremely low toxicity.
  • the many compounds of this invention are markedly low in toxicity, and even when over 1,000 mg./kg. of these compounds are orally administered to each of rat and mouse, they scarcely 32 yield 20 g. acetaldehyde N -(phenylacetyl)-N-(p methoxyphenyDhydrazone, M.P. 9810l C. Y
  • the present inventors prepared many other 1-acyl-3- indolyl aliphatic acid derivatives than the compounds shown in the aforesaid table and evaluated the pharmaceutical effects thereof by animal tests.
  • the present inventors have found that many derivatives (I) of 1-acyl-3-indolyl aliphatic acid, which are prepared by the present invention, are superior to l-(pchlorobenzoyl) 2 methyl 5 methoxy 3 indolylacetic acid (Indomethacin) and 1,2-diphenyl-3,5-dioxo-4- n-butylpyrazolidine (Phenylbutazone) in the therapeutic ratios thereof and has a great practical value.
  • EXAMPLE 1 13.8 g. of phenylacetyl chloride was dropped to 13.1 g. of acetaldehyde N -(p-rnethoxyphenyl)hydrazone in 50 ml. of pyridine under cooling with ice. Thereafter stirring of the reaction mixture was continued for additional overnight under cooling with ice, and then it was poured into 250 ml. of cold water. As a result, a large amount of crystals were produced. The resultant crystals were collected by filtration, washed with water, and dried to EXAMPLE 6 Acetaldehyde N (a naphthylacetyl) N p methoxyphenyl)hydrazone, M.P. -103 C.
  • EXAMPLE 10 EXAMPLE 11 Acetaldehyde N (p methoxycinnamoyl) -N -'(p-methoxyphenyl)hydrazone, M.P. 172179 C.
  • EXAMPLE 15 EXAMPLE 16 N (m-nitrocinnamoyl)-N -(p-methoxyphenyl)hydrazine hydrochloride, M.P. 165 l70 C. (decomp.).
  • EXAMPLE 36 58 g. of acetaldehyde N -(B-T-furylacryloyl)-N -(pmethoxyphenyl)hydrazone was suspended in 400 m1. of ethanol, and the suspension mixture was shaken under cooling with ice.
  • EXAMPLE 37 7.7 g. of phenylacetyl chloride was dropwise added to the solution mixture of 6.1 g. of p-tolylhydrazine and 5 g. of triethylamine in ml. of toluene under cooling with ice.
  • EXAMPLE 38 6.0 g. of cinnamoyl chloride was dropwise added to a mixture of 8.7 g. of p-methoxyphenylhydrazine hydrochloride, 10.1 g. of triethylamine and 200 ml. of toluene under cooling at 5 to 0 C.
  • EXAMPLE 50 1 cinnamoyl-Z-methyl-5-methoxy-3-indolylacetic acid, M.P. l64-165 C.
  • EXAMPLE 54 1 (p methoxycinnamoyl)-2-methyl-5-methoxy-3-indolylacetic acid, M.P. 193-l95 C.
  • EXAMPLE 55 1- (p-chlorophenylacetyl) 2 methyl 5 methoxy-3-indolylacetic acid, M.P. l80-l82 C.
  • EXAMPLE 56 Ethyl l-cinnamoyl-Z-methyl-5-methoxy-3-indolylacetate, M.P. 162163 C.
  • EXAMPLE 59 1 (a. chlorophenylacetyl)-2-methyl-'5-methoxy3- indolylacetic acid, M.P. 165 166 C.
  • EXAMPLE 60 20 g. of N (phenylacetyl)-N -(p-methoxypl1enyl)- hydrazine hydrochloride was added to 30 g. of levulinic acid. The mixture was heated at 76 C. for 3 hours, and then allowed to stand at room temperature overnight. The precipitates were collected by filtration, washed with water and dried to give crude crystals of 1-(phenylacetyl)- Z-methyl-5-methoxy-3-indolylacetic acid, which was recrystallized from acetone-water to give 9.7 g. of a purified product, M.P. 14l.5l43 C.
  • l-(phenylacetyl)-2-methyl- 5-methoxy-3-indoly1acetic acid was prepared in a similar yield from a mixture of 10 g. of levulinic acid and 25 ml. of acetic acid instead of 30 g. of levulinic acid.
  • EXAMPLE 61 A mixture of 10 g. of N -(diphenylacetyl)-N -(pmethoxyphenyDhydrazine hydrochloride and 20 g. of levulinic acid was heated at 75 83 C. for 2.5 hours with stirring. After the completion of reaction, the reaction mixture was poured into water, and then precipitate was produced. It was collected by filtration, Washed with water and recrystallized from acetone-water to give light gray crystals of 1-diphenylacetyl-Z-methyl-S-methoxy-3- indolylacetic acid; M.P. l5l C.
  • EXAMPLE 62 In a way similar to that of Example 61, l-(u-chlorophenylacetyl) 2-methyl-5-methoxy-3-indolylacetic acid, M.P. l66 C., was prepared from N (0c-Ch10IO- phenylacetyl) N -(p-methoxyphenyl)hydrazine hydrochloride and levulinic acid.
  • EXAMPLE 63 In a way similar to that in Example 61 l-(a-phenylbutyroyl)-2-methyl-5-methoxy-3-indolylacetic acid, M.P. 123.5 -125 C. was prepared from N -(u-phenylbutyroyl) -N (p-methoxyphenyl) hydrazine hydrochloride and levulinic acid.
  • EXAMPLE 64 In a way similar to that in Example 61, l-(ot-naphthylacetyl) 2-methyl-5-methoxy-3-indolylacetic acid, M.P. 165.5-166.5 C, was prepared from N -(a-naphthylacetyl) N (p-methoxyphenynhydrazine hydrochloride and levulinic acid.
  • EXAMPLE 65 A mixture of 10 g, of N -(diphenylacetyl)-N -(pmethoxyphenyl)hydrazine hydrochloride and 20 g. of ethyl levulinate was heated in 20 ml. of ethanol at 80- 83 C. for 6.5 hours with stirring. After completion of the reaction, the mixture was allowed to cool and poured into Water to give a dark blue homogenous solution,
  • EXAMPLE 66 In a way similar to that in Example 61, l-(p-chlorophenylacetyl) 2-methyl-5-methoxy-3-indolylacetic acid, M.P. 178 C., was prepared from g. of N -(p-chlorophenylacetyl) N -(p-methoxyphenyl)hydrazine hydrochloride and 30 ml. of levulinic acid.
  • EXAMPLE 67 5 g. of N (p-nitrophenylacetyl)-N -(p-methoxyphenyl)hydrazine hydrochloride and 10 g. of levulinic acid were added to 30 ml. of acetic acid. The mixture was heated at 9095 C. for 2 hours under stirring. Thereafter the mixture was poured into 150 ml. of water, and the resultant precipitates were collected by filtration, washed with water and recrystallized from dioxane to give 1 (p-nitrophenylacetyl)-2-methyl-5-methoxy-3-indolylacetic acid, M.P. 207 C.
  • EXAMPLE 68 In a way similar to that in Example 61, 1('y-p-methoxyphenyl-n-butyroyl) 2 methyl-5-methoxy-3-indolyl acetic acid, M.P. 163 C., was prepared from N -(v-pmethoxyphenyl-n-butyroyl) N (p-methoxypheny1)- hydrazine sulfate and levulinic acid.
  • EXAMPLE 69 In a way similar to that in Example 61, 1-m,p-dimethoxyphenylacetyl) 2 methyl-S-methoxy-3-indolylacetic acid, M.P. 169-170 C., was prepared from N -(m,p-dimethoxyphenylacetyl) N -(p-methoxyphenyl)hydrazine hydrochloride and levulinic acid.
  • EXAMPLE 70 In a way similar to that in Example 61, 1-(,8-phenyl-npropionyl)-2-methy1-5-methoxy-3-indolylacetic acid, M.P. 163164 C., was prepared from N -(fi-phenyl-n-propionyl) N -(p-methoxyphenyl)hydrazine hydrochloride and levulinic acid.
  • EXAMPLE 71 30 g. of N -cinnamoyl-N -(p-methoxyphenyl)hydrazine hydrochloride was added into 50 g. of levulinic acid, and the mixture was heated at 75 C. for 2 hours under stirring. Thereafter the reaction mixture was poured into 200 ml. of water under vigorous stirring, the resultant crystals were collected by filtration, dried to give 34 g. of crude crystals of 1 cinnamoyl-2-methyl-5-methoxy-3-indolylacetic acid, M.P. 158-160 C., which was recrystallized from acetone twice. The melting point was raised to 164-165 C.
  • EXAMPLE 72 In a way similar to that in Example 71, l-(B-p-tolylacryloyl 2-methyl-5-methoxy-3-indolylacetic acid, M.P. 195 C., was prepared by reacting N (fl-p-tolyl-acryloyD- N (p methoxy-phenyDhydrazine hydrochloride with levulinic acid.
  • EXAMPLE 74 According to a method similar to that in Example 71, 1 (u-methylcinnamoyl) 2-methyl-5-methoxy-3-indolylacetic acid, M.P. 153.5 154.5 C., was prepared by reacting N -(wmethylcinnamoyl)-N -(p-methoxyphenyl) hydrazine hydrochloride with levulinic acid.
  • EXAMPLE 75 20 g. of N -cinnamoyl-N -(p-methoxyphenyl)hydrazine hydrochloride and 12 g. of v-acetyl-n-butyric acid were added to 30 ml. of acetic acid, and the mixture was stirred at 75 C. for 2 hours. After completion of the reaction, the reaction mixture was allowed to cool, poured into 150 ml. of Water, and the resultant precipitates were collected by filtration, washed with water and dried to give 18 g. of crude crystals of p-(1-cinnamoyl-2-methyl-5-methoxy-3- indolyl)propionic acid, M.P. 182-186 C. The crude product was recrystallized from acetone-water to give 9.3 g. of the pure product, whereby the melting point was raised to 189190 C.
  • EXAMPLE 76 In a way similar to that in Example 75, a-(Lcinnamoyl- 2 methyl 5-methoxy-3-indolyl)butyric acid, M.P. 126 C., was prepared by reacting N -cinnamoyl-N -(pmethoxyphenyl)hydrazine hydrochloride with e-acetyl-nvaleric acid.
  • EXAMPLE 77 In a way similar to that in Example 75, yellow needle crystals of 1 (m-nitrocinnamoyl)-2-methyl-5-methoxy- 3-indolylacetic acid, M.P. 203 204 C., was prepared by reacting N -(m nitrocinnamoyl)-N -(p-methoxyphenyl) hydrazine hydrochloride with levulinic acid.
  • EXAMPLE 78 5 g. of N -(fl-Z-furylacryloyl)-N -(p-methoxyphenyl)- hydrazine hydrochloride was added to 15 g. of levulinic acid, and the mixture was stirred at 75 80 C. for 2 hours. Thereafter, the mixture was allowed to cool and poured into cooled water. The resultant brown precipitates were collected by filtration, washed with water thoroughly. The washing liquor and the precipitates were combined and ether was added thereto, and then the mixture was shaken, and the ether layer Was separated.
  • EXAMPLE 79 10 g. of N -cinnamoyl-N -(p-methoxyphenyl)hydrazine hydrochloride and 20 g. of a-methyl levulinic acid were added to 10 ml. of acetic acid and the mixture was stirred at 80 C. for 2 hours. The reaction mixture was allowed to stand to cool, and poured into water to give an oily substance, which was washed with water thoroughly, and extracted with each 100 ml. of ether three times.
  • EXAMPLE 80 In a way similar to that in Example 75, l-(p-methoxycinnamoyl)-2-methyl-5-methoxy-3-indolylacetic acid, M.P. 193195 C., was prepared from N -(p-methoxycinnamoyl)-N (p-methoxyphenyl)hydrazine hydrochloride and levulinic acid.
  • EXAMPLE 81 In a way similar to that in Example 71, ethyl l-cinnamoyl-2,5-dimethyl-3-indolylacetate, M.P. 198200 C., was prepared from N -cinnamoy1-N -(p-tolyl)hydrazine hydrochloride and ethyl levulinate.
  • EXAMPLE 82 In a Way similar to that in Example 71, l-ot-phenylcinnamoyl-Z-methyl-S-methOxy-3-indolylacetic acid, M.P. 174175 C., was prepared from N -ot-phenyl-cinnamoyl- N -(p-methoxyphenyl)hydrazine hydrochloride and levulinic acid.
  • EXAMPLE 83 According to a method similar to that in Example 71, 1-pheny1acetyl-2-methyl-5-methoxy 3 indolylacetic acid, M.P. 137l 39 C., was prepared from N -(phenylacetyl)- N -(p-methoxyphenyl)hydrazine hydrochloride and levulinic acid.
  • EXAMPLE 84 In a way similar to that in Example 71, l-(p-chlorocinnamoyl)-2-methyl-5-methoxy-3-indolylacetic acid, M.P. 220221 C., was prepared from N -(p-chlorocinnamoyl)- N -(p-methoxyphenyl)hydrazine hydrochloride and levulinic acid.
  • EXAMPLE 85 A mixture of 4.0 g. of N -(cc-chlorophenylacetyl)-N (p-rnethoxyphenyl)hydrazine hydrochloride and 2.4 g. of acetonylmalonic acid was heated in ml. of acetic acid at 85 C. for 4 hours with stirring. Thereafter, the reaction mixture was allowed to cool, and was poured into ml. of cold water, and then crystals were produced. They were collected by filtration, and dried to give crude product of 1-(a-chlorophenylacetyl)-2-rnethyl-5-methoxy 3 indolylacetic acid. The recrystallization from acetone-water gave fine white needle crystals, M.P. l65-166 C.
  • EXAMPLE 90 In a way similar to that in Example 89, 1-cinnamoy1-2- methyl-5-methoxy-3-indolylacetic acid, M.P. 164165 C., was prepared from N -cinnamoyl-N -(p-methoxyphenyl)hydrazine hydrochloride and acetosuccinic acid.
  • EXAMPLE 91 4.8 g. of tertiary butyl l-(phenylacetyl)-2-methyl-5- methoxy-3-indolylacetate was added to 40 m1. of benzene, then a small amount of p-toluenesulfonic acid was added thereto. The reaction mixture was refluxed to complete the reaction. After the heating, the mixture was left at room temperature. After cooling it was washed with 60 ml. of water twice, and was dried.
  • reaction s lvent was removed by distillation, and the resultant residue was purified and crystallized by using acetone and recrystallized from acetone-water to give l-(phenylacetyl)-2-methyl-5- methoxy-3-indolylacetic acid, M.P. 138139 C.
  • EXAMPLE 100 7.6 g. of acetaldehyde N -phenylacetyl-N -(p-methoxyphenyl)hydrazone was added to 30* g. of levulinic acid containing 1 g. of hydrogen chloride, and the mixture was heated at 80 C. for 3 hours. Thereafter, the mixture was allowed to cool, and poured into 200* ml. of water. And then the solid produced was collected by filtration, and was column-chromatographed on silica gel by using ethyl acetate as developer and then was re-

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Abstract

A 1-ACYL-3-INDOLYL ALIPHATIC ACID DERIVATIVE HAVING REMARKABLE ANTI-INFLAMMATORY, ANTIPYRETIC AND ANALGESIC ACTIONS WHICH IS CHARACTERIZED IN THAT SAID ACYL SUBSTITUTENT IS ARALKYLCARBONYL OR ARALKENYLCARBONYL WHOSE CARBON CHAIN HAS UP TO 5 CARBON ATOMS, AND PROCESS FOR PRODUCING THE COMPOUND.

Description

3,576,800 Patented Apr. 27, 1971 ice United States Patent 3 576 800 17621 (1961), J. Gen. Chem, U.S.S.R., 28, 1058 (1958)] LACYLSJNDOLYL ALIHATIC ACID DERIVA have recently reported this problem as follows:
TIVES AND THEIR METHOD OF PREPARATIUN R Hisao Yamamoto, Nishinomiya-shi, and Masam Nakao, GHFCHQ CH CH3 Osaka, Japan, assignors to Sumitomo Chemical Com- 5 H pany, Ltd., Osaka, Japan T N a No Drawing. Filed May 2, 1967, Ser. No. 635,362 Ac Ac Claims priority, application Japan, May 12, 1966,
41/311,306; June 27, 1966, 41/ 12,039; June 30, 1966, 41/ 42,721, 41/42,272; July 8, 1966, 41/ 44,723, ll/44,724; Aug. 1 1966, 41/511,691; Aug. CH CH3 19, 1966, 41/5 1,675, il/54,674; Dec. 15, 1966, R 0133 il/82,480; Dec. 16, 1966, ll/82,649, ll/82,650; Dec. 20, 1966, 4l/83,748; Jan. 6, 1967, 42/1,351, NHAc CH 42/1,352; Jan. 7, 1967, 42/1,499; Jan. 16, 1967, g 42/3,223, ll/3,224; Jan. 17, 1967, 42/3,530, 42 3531 They have explained that an important requirement for Int. Cl. (107d 27/56 iridole formation is the deacylation of the N -acy1 group US. Cl. 260-240 19 C a ms of hydrazine derivative, which frees a p-electron pair on the N -nitrogen atom. ABSTRACT OF THE DISCLOSURE 20 SUMMARY OF THE INVENTION A 1-acyl-3-indolyl aliphatic acid derivative having re- P e f e found that hVe1 l y y markable anti-inflammatory, antipyretic and analgesic ahlfhauc jacld derlvatlves havlhg h hhh'lhflemmatory, actions which is characterized in that said acyl substituent antl'pyretlc and analgeslc actlvltles were P p y is aralkylcarbonyl or aralkenylcarbonyl whose carbon novel or known proeesseschain has up to 5 carbon atoms, and process for producone hl of e P e h on Is to prov de novel i the compound 1-acyl-3-1ndolyl aliphatic acid der1vat1ves having high anti-inflammatory, anti-pyretic and analgesic activities and processes for producing such derivatives. Another BACKGROUND OF THE INVENTION object of the present invention is to provide processes for economically manufacturing these compounds in a Fleld of the lhvehhoh high yield. A further object of the present invention is The present invention relates to novel N-substituted to Provide a novel Pharmaceutical f pe containing indole derivatives having high anti-inflammatory, antithese compounds as the efiectlve lhgfedleht- Still f t pyretic and analgesic activities and to processes for proohleets W111 he -PP h from e fOnOWlhg desenlehohducing the same. More particularly, the present invention In Order to aeeomphsh these l the P e Y relates to novel 1-acyl-3-indolyl aliphatic acid derivatives h Preludes hovel y ahPhahe field derlvaand processes for producing the same. fives 0f the formula? (2) Prior art 40 1 (1'1 Of the developed non-steroidal anti-inflammatory com- 4 3 CH m (CH2)n O p 0 pounds, 1-(p-chlorobenzoyl)-2-methyl-5-meth0xy-3indo- 5 lylacetic acid is greatest in activity. But it is high in toX- R-- icity. The present inventors also observed that even when 6 1 5 l0 mg./kg. of said compound was orally administered, a I V rat showed an occult bleeding. In addition thereto, all the C0 conventional anti-inflammatory compounds tend to pro- A mote the bleeding of digestive organs and not few ex- 1 (I) amples have been reported that perfolations of the stomach and intestines brought one to death. 'Further wherein R is an unsubstituted or a lower alkyl-, lower 1,2 diphenyl 3,5 dioxo 4 n butylpyrazolidine alkoXy-, lower alkylthio-, nitro-, cyanoor halogen- (phenylbutazone), which is most widely used as antisubstituted aromatic ring group, each of said alkyl, alkoxy phlogistic at present, has low activity in comparison to and alkylthio substituents containing up to 4 carbon its high acute toxicity and hence is considerably small atoms, or an unsubstituted or a methyl-, ethylor halogenin therapeutic ratio. 5 substituted 5- or 6-membered heterocyclic ring group The synthesis of indole derivatives having acyl groups containing an oxygen, sulfur or nitrogen atom; R and at the N-positions is described in, for'example, Elderfield: R each are hydrogen atoms or alkyl groups having up Heterocyclic Compounds, vol. 3 (1952), chapter 1, to 3 carbon atoms; R is a hydrogen atom, a carboxy pages 1-247, and W. C. Sumpter and F. M. Miller: group or an alkoxycarbonyl group having up to 4 carbon Heterocyclic Compounds with Indole and Carbazole atoms; R is an alkoxy group having up to 4 carbon Systems (1954), pages 1-69. l-Substituted acyl groups atoms, a benzyloxy group, a tetrahydropyranyloxy group, of l-acyl-indole derivatives are so easily hydrolyzed by an amino group or a hydroxy group; R is an alkyl group acid or alkali that it has been considered impossible to having up to 4 carbon atoms, an alkoxy group having up obtain l-acyl-indole derivatives directly from correspond to 4 carbon atoms, an alkylthio group having up to 4 ing N -acylated phenylhydrazine derivatives by Fischers carbon atoms, a nitro group, an alkenyl group having indolization. Suvorov et a1. [Suvorov et a1.: Doklady up to 4 carbon atoms, an alkenyloxy group having up Acad. Nauk S.S.S.R. 136, 840 (1961), Chem. Abstn, 55, to 4 carbon atoms, a halogen atom or a hydrogen atom;
A is an unsubstituted saturated hydrocarbon chain having up to 5 carbon atoms, an unsubstituted unsaturated hydrocarbon chain having up to 5 carbon atoms, a halogen-substituted saturated hydrocarbon chain having up to 5 carbon atoms, a halogen-substituted unsaturated hydrocarbon chain having up to 5 carbon atoms, a phenylsubstituted saturated hydrocarbon chain having up to 5 carbon atoms or a phenyl-substituted unsaturated hydrocarbon chain having up to 5 carbon atoms, the hydrocarbon chain being a straight one or a branched one; m and p each are or 1; and n is 0 or an integer of from 1 to 3.
Further, the present invention provides a process for producing novel l-acyl-S-indolyl aliphatic acid derivatives represented by the Formula 1, which comprises reacting an N -acylated phenylhydrazine derivative of the formula:
III! (II) werein R R and A have the same meanings as defined above, with an aliphatic acid derivative of the formula:
wherein R R R R m, n and p have the same meanings as identified above.
Still further, the present invention provides a process for producing novel 1-acyl-3-indolyl aliphatic acid derivatives represented by the Formula I, which comprises decomposing an N -acylated phenylhydrazone derivative of the formula:
R (IV) fied above,, and B is a ketone or aldehyde residue, with a decomposing agent to yield an N -acylated phenylhydrazine derivative of the formula:
wherein R and B have the same meanings as identified above, with a compound having the formula:
wherein R and A have the same meanings as identified above and Y represents a halogen or an ester residue, to yield an N -acylated phenylhydrazone derivative represented by the Formula IV, decomposing the resultant N -acylated phenylhydrazone derivative with a decomposing agent to give an N -acylated phenylhydrazine derivative represented by the Formula II, and reacting the resultant N -acylated phenylhydrazine derivative with an aliphatic acid derivative represented by the Formula III to yield the 1-acy1-3-indolyl aliphatic acid derivative (I).
Still further, the present invention provides a process for producing novel l-acyl-3-indolyl aliphatic acid derivatives of the Formula I; which comprises reacting a phenylhydrazone derivative of the Formula V with a compound having the Formula VI to yield an N -acylated phenylhydrazine derivative of the Formula II, and reacting the resultant N -acylated phenylhydrazine derivative of the Formula II with an aliphatic acid compound of the Formula III to yield an 1-acy1-3-indolyl aliphatic acid derivative (1).
Still further, the present invention provides a process for producing novel 1-acyl-3-indolyl aliphatic acid derivatives of the Formula I, which comprises reacting an N -acylated phenylhydrazone derivative of the Formula IV with an aliphatic acid derivative of the Formula III to yield the l-acyl-S-indolyl aliphatic acid derivative of the Formula I.
Still further, the present invention provides a process for producing novel l-acyl-3-indolyl aliphatic acid derivatives of the Formula I, which comprises reacting a phenylhydrazone derivative of the Formula V with a compound having the Formula VI to yield an N -acylated phenylhydrazone derivative of the Formula IV and reacting the resultant N -acylated phenylhydrazone derivative (IV) with an aliphatic acid derivative of, the Formula III to yield the l-acyl-3-indolyl aliphatic acid derivative (I).
Still further, the present invention provides a process for producing novel 1-acyl-3-indoly1 aliphatic acid derivatives of the Formula VII:
1 (VII) wherein R R R R A and m have the same meanings as identified above, which comprises reacting an N -acylated phenylhydrazine derivative of the Formula II with a compound of the formula:
COOH (VIII) wherein R R and m have the same meanings as identified above, to yield the l-acyl-3-indolyl aliphatic acid derivative (VII).
Still further, the present invention provides a process for producing novel l-acyl-3-indolyl aliphatic acid derivatives of the Formula VII, which comprises reacting an N -acylated phenylhydrazone derivative of the Formula IV with a compound of the Formula VIII to yield the lacyl-3-indoly1 aliphatic acid derivative of the Formula VII. Still further, the present invention provides a process for producing novel 1-acyl3-indolylacetic acid derivatives wherein R R and A have the same meanings as identified above, which comprises reacting an N -acylated phenylhydrazine derivative of the Formula II with acetosuccinic acid or 2-ketoadipic acid to yield the l-acyl-3-indolylacetic acid derivative of the Formula IX.
Still further, the present invention provides a process for producing novel 1-acyl-3-indolylacetic acid derivatives of the Formula IX, which comprises reacting an N acylated phenylhydrazone derivative of the Formula IV with acetosuccinic acid or 2-ketoadipic acid to yield the 1-acyl-3-indoly1acetic acid (IX).
Still further, the present invention provides a process for producing novel l-acyl-3-indolyl aliphatic acid derivatives of the Formula I, which comprises reacting a phenylhydrazine derivative of the formula:
wherein R has the same meaning as identified above, with a compound having the Formula VI to yield an N acylated phenylhydrazine derivative of the Formula II, and reacting the resultant N -acylated phenylhydrazine derivative (II) with an aliphatic acid compound of the Formula III to yield the 1-acyl-3-indolyl aliphatic acid derivative (I).
Still further, the present invention provides a process for producing novel 1-acyl-3-indolyl aliphatic acid derivatives of the Formula I, which comprises reacting a phenylhydrazine derivative of the Formula XI:
wherein R R and A have the same meaning as identified in the Formula I, with an aliphatic acid derivative (III).
Still further, the present invention provides a process for preparing novel l-acyl-3-indolyl aliphatic acid derivatives represented by the formula:
1 (XII) 6 wherein R R R R A, m and n have the same meanings as identified in the Formula I, which comprises converting a derivative represented by the formula:
(XIII) wherein R R R R A, m and n have the same meanings as identified above; and R represents tertiary butyloxy, tetrahydropyranyloxy, benzyloxy or amino, into the said 1-acyl-3-indolyl aliphatic acid derivative (XII).
Still further, the present invention provides a process for producing novel 1-acyl-3-indoly1 aliphatic acid derivatives of the Formula XII, which comprises reacting an N -acylated phenylhydrazine derivative of the Formula II with a compound of the formula:
wherein R R R R and A have the same meanings as identified above, which comprises oxidizing an indole-3- aliphatic aldehyde derivative represented by the formula:
l CIICHO (XVI) wherein R R R R and A have the same meanings as identified above.
Still further, the present invention provides a process for producing novel 1-acyl-3-indolyl aliphatic acid derivatives of the Formula XV, which comprises reacting an N acylated phenylhydrazine derivative of the Formula II with a compound of the formula:
wherein R and R have the same meanings as identified above and R is a lower alkyl group, to yield a 3-indolyl aliphatic aldehyde acetal derivative of the formula:
R3 -(JHCH(ORB)2 R (XVIII) wherein R R R R R and A have the same meanings as identified above, decomposing the resultant 3-indolyl aliphatic aldehyde acetal derivative to yield a 3-indolyl aliphatic aldehyde derivative of the Formula XVI, and oxidizing the resultant 3-indolyl aliphatic aldehyde derivative to yield the l-acyl-3-indolyl aliphatic acid derivative (XV).
Still further, the present invention provides a process for preparing novel 1-acyl-3-indolyl aliphatic acid derivatives of the formula:
I O t R (XV) wherein R R R R and A have the same meanings as identified above, which comprises oxidizing an indole-3- ethanol of the formula:
R3 hHoH2oH ('10 l 1'1 (XIX) wherein R R R R and A have the same meanings as identified above, to yield an 1-acyl-3-indo1yl aliphatic acid derivative of the Formula XV.
Still further, the present invention provides a process for producing novel 1-acyl-3-indolyl aliphatic acid derivatives of the Formula XV which comprises reacting an N -acylated phenylhydrazine derivative of the Formula II with a compound of the formula:
R3 R?-C OCH2-( JH-CHzOI-I (XX) wherein R and R have the same meanings as identified above, to yield a 1-acyl-3-indolyl aliphatic alcohol derivative of the Formula XIX and oxidizing the resultant 1-acy1-3-indolyl aliphatic alcohol derivative (XIX) to yield the 1-acyl-3-indolyl aliphatic acid derivative (XV).
Still further, the present invention provides a process for preparing novel l-acyl-3-indolyl aliphatic acid derivatives of the Formula XV Which comprises dehydrogenating a 2,3-dihydro-3-indolyl aliphatic acid derivative of the formula:
l (XXI) 8 wherein R R R R and A have the same meanings as identified above.
Still further, the present invention provides a process for preparing novel l-acyl-3-indolylacetic acid derivatives of the formula:
R1 (XXII) wherein R R R R and A have the same meanings as identified above, which comprises dehydrating or dehydrating and hydrolyzing thereafter a 2-hydro-3-hydroxy-3- indolylacetic acid derivatives of the formula:
I CO I R (XXIII) wnerein R R R R and A have the same meanings as identified above.
Still further, the present invention provides a process for preparing novel l-acyl-3-indolyl aliphatic acid derivatives of the Formula XV which comprises subjecting a(N- acylanilino)-aliphatic acid derivatives of the formula:
0 R3 dim-00011 N-CH ('10 R l 1 1 (XXIV) wherein R R R R and A have the same meanings as identified above, to ring formation reaction.
Still further, the present invention provides a process for preparing novel 1-acyl-3-indolylacetic acid derivatives of the Formula IX, which comprises heating a 3-(2'- acylaminophenyl) -levulinic acid derivative of the formula:
l C O RI (XXV) wherein R R and A have the same meanings as identi fied above, in the presence of an inorganic acid in a suitable solvent.
According to the present invention, a 1-acyl-3-indoly1 aliphatic acid derivative represented by the Formula I is prepared by the reactions shown by the following equations:
Acetaldehyde N -cinnamoyl-N -(p-methylthiophenyl) hydrazone Acetaldehyde N -cinnamoyl-N -(p-ethoxyphenyl) hydrazone Acetaldehyde N (5 '-phenyl-2',4-pentadienoyl) -N (p-rnethoxyphenyl)hydrazone In the above formulas, R R R R R R", A, B, Y, m, n and p have the same meanings as identified above.
In the processes of the present invention N -acylated phenylhydrazine derivatives (II) and N -acylated phenylhydrazone derivatives (IV) may be synthesized as intermediates by other processes than those disclosed herein. These intermediates, the compounds (II) and (IV), are novel compounds.
Next, the process of the present invention is explained in due order asjollows.
Firstly, the reaction of a phenylhydrazone derivative (V) and a compound (VI) will be described.
The reaction of a phenylhydrazone derivative (V) with a. compound (VI) is carried out in the presence of a hydrogen halide acceptor. As the hydrogen halide acceptor, a tertiary amine, for example, pyridine or dimethylaniline can be used. These hydrogen halide acceptors themselves can be used as solvents. Inert solvents such as ether, benzene, toluene, and tetrahydrofuran are also able to be used as reaction solvents in the presence of equimolar or larger amounts of these hydrogen halide acceptors. The compound (VI), may be chloride, bromide, iodide or fluoride and chloride is most preferable from a commercial point of view. The reaction proceeds at room temperature in many cases, and even below 0 C. in some kind of solvent used. The exothermic reaction finishes in a few minutes or several hours. After the reaction finishes, the produced hydrogen halide salt of the hydrogen halide acceptor is filtered off and the filtrate is concentrated under a reduced pressure, or the reaction mixture is poured into water when a water-soluble solvent like pyridine is used as the solvent. And then the aimed N -acylated phenylhydrazone compound is easily obtained as crystals or an oily substance. These products can be purified with an appropriate solvent, for example, the solvent mixture of alcohol and water.
In case a compound having a comparatively weak N=C bonding is used as a derivative (V) or under severe conditions of reaction, an N -acylated phenylhydrazine derivative (II) is directly obtained in place of an N -acylated phenylhydrazone derivative (IV).
According to the method of the present invention, the following compounds can be obtained in a high yield. As the N -acylated phenylhydrazone compounds (IV), there are illustrated:
Acetaldehyde N -cinnamoy1-N -(p-methoxyphenyl) hydrazone Acetaldehyde N -cinamoyl-N -(p-methylphenyl) hydrazone Acetaldehyde N -cinnarnoyl-N -(p-chlorophenyl) hydrazone Acetaldehyde N 5 '-phenyl-2',4'-pentadienoyl) -N (p-ethoxyphenyl)hydrazone Acetaldehyde N -cinnamoyl-N -(m-methoxyphenyl) hydrazone Acetaldehyde N -cinnarnoyl-N (m-tolyl)hydrazone Acetaldehyde N (4-phenyl-3 -butenoyl -N (pmethoxyphenyl hydrazone Benzaldehyde N -cinnamoyl-N -(p-methoxyphenyl) hydrazone Chloral N -cinnamoyl-N (p-methoxyphenyl hydrazone Acetophenone N -cinnamoyl-N -(p-methoxyphenyl) hydrazone Acetaldehyde N (,8-2'-furylacryloyl -N (p-methoxyphenyl hydrazone Acetaldehyde N (fi-2'-furylacryloyl) -N -(p-methy1- phenyl)hydrazone Acetaldehyde N ,B-2'-furylacryloyl -N (p-chlorophenyl)hydrazone Acetaldehyde N fl-2'-furylacryloyl) -N (p-methylthiophenyl)hydrazone Acetaldehyde N (fl-2furylacryloyl)-N -(p-ethoxyphenyl)hydrazone Acetaldehyde N a-naphthylacryloyl -N (p-methoxyphenyl)hydrazone Acetaldehyde N -(a-naphthylacryloy1)-N -(p-ethoxyphenyl hydrazone Acetaldehyde N -(,8-2-thienylacryloyl)-N -(m-rneth0xyphenyl)hydrazone Acetaldehyde N (,8-2'-thieny1acryloyl) -N (m-tolyl) hydrazone Acetaldehyde N (fi-2-pyridylacryloyl -N (p-methoxyphenyl)hydrazone Benzaldehyde N (,8-2-thienylacryloyl -N (p-methoxyphenyl)hydrazone Chloral N -(fi-2-thienylacryloyl)-N -(p-methoXyphenyl)hydrazone Acetophenone N -(fl-2'-thienylacryloyl) -N -(p-methoxyphenyl)hydrazone Acetaldehyde N -(m-nitrocinnam0y1)-N -(p-methoxyphenyl)hydrazone Acetaldehyde N (m-nitrocinnamoyl -N (p-methylphenyl)hydrazone Acetaldehyde N -(m-nitrocinnamoyl)-N -(p-chlorophenyl)hydrazone Acetaldehyde N (m-nitrocinnamoyl) -N (p-methylthiophenyl)hydrazone Acetaldehyde N -(m-nitrocinnamoyl)-N -(p-ethoxyphenyl)hydrazone Acetaldehyde N (p-methoxycinnamoyl)-N -(p-methoxyphenyl)hydrazone Benzaldehyde N -cinnamoyl-N -(p-methoxyphenyl) hydrazone Chloral N (p-methoxycinnamoyl) -N (p-methoxypheny1)hydrazone Acetophenone N (p-methoxycinnamoyl) -N (p-methoxyphenyl)hydrazone Acetaldehyde N -(-p-chlorophenyl-2', -pentadienoyl)- N -(p-methoxyphenyl)hydrazone Acetaldehyde N a-phenyl-p-chlorocinnamoyl -N p-methoxyphenyl)hydrazone Acetaldehyde N -(,8-p-tolylacryloy1)-N -(p-methoXyphenyl hydrazone Acetaldehyde N -(a-methyl-p-chlorocinnamoyl)-N (p-methoxyphenyl)hydrazone Acetaldehyde N (p-chlorocinnamoyl) -N (p-rnethoxyphenyl hydrazone Acetaldehyde N (p-methylthiocinnamoyl -N (p-methoxyphenyl)hydrazone Acetaldehyde N -(p-chlorocinnamoyl)-N -(p-methoxyphenyl hydrazone Acetaldehyde N (phenylacetyl) -N (p-methoxyphenyl) hydrazone Acetaldehyde N (p-tolylacetyl) -N -(p-methoxypheny1) hydrazone Acetaldehyde N phenylacetyl) -N -(p-methylphenyl) hydrazone Acetaldehyde N 5-phenyl-n-pentanoyl) -N (p-methoxyphenyl)hydrazone Acetaldehyde N (phenylacetyl) 'N (m-chlorophenyl) hydrazone Acetaldehyde N (phenylacetyl) -N -(p-chloropheny1) hydrazone Acetaldehyde N (phenylacetyl)N (p-rnethylthiophenyl) hydrazone Acetaldehyde N -(p-chlorobenzylacetyl)N -(p-methoxyphenyl) hydrazone Acetaldehyde N -(p-methylphenylacetyl)-N -(m-methoxyphenyl hydrazone Acetaldehyde N -(p-methoxyphenylacetyl)-N -(p-methoxyphenyDhydrazone Acetaldehyde N -(a-phenyl-n-butyroyl)-N -(p-methoxyphenyl)hydrazone Actaldehyde N (3 -pyridy1acetyl)-N -(p-methoxyphenyl) hydrazone Acetaldehyde N -(2'-pyridylacetyl)-N (p-methoxyphenyl) hydrazone Acetaldehyde N -(4'-pyridylacety1)-N -(p-methoxyphenyl hydrazone Acetaldehyde N 2'-thienylacetyl) -N (p-methoxyphenyl) hydrazone Acetaldehyde N 2-furylacetyl) -N -(p-methoxyphenyl) hydrazone Acetaldehyde N (diphenylacetyl -N (p-rnethoxyphenyl) hydrazone Acetaldehyde N (phenylacetyl) -N -(rn-methoxypheny1) hydrazone The following N -acylated hydrazine derivatives can be very easily obtained directly from hydrazone derivative of ethyl levulinate, ethyl acetoacetate, methyl 4-methoxy- 3-oxo-n-butyrate, etc., under some conditions of the reaction:
N -cinnamoyl-N (p-methoxyphenyl hydrazine N -cinnamoy1-N -(p-tolyl)hydrazine N -cinnamoyl-N -(m-methoxyphenyl)hydrazine N -(m-nitrocinnamoy1) -N -(p-methoxyphenyl) hydrazine 1 2 N phenylacetyl) -N p-methoxyphenyl) hydrazine N (p-tolylacetyl) -N (p-methoxyphenyl) hydrazine N 2'-furylacetyl N (p-rnethoxyphenyl) hydrazine Those novel N -acylated phenylhydrazone derivatives and N -acylated phenylhydrazine derivatives which are obtained by the method of the present invention have psychic, stimulating, anti-tumor, bactericidal, and fungicidal effects and they are very important compounds as intermediates for producing remarkably effective antiinflammatory drugs, analgesics and anti-pyretics.
Next, the process for producing an N -acylated phenylhydrazine derivative (II) by decomposing an N -acylated phenylhydrazone derivative (IV) Will be described.
An N -acylated phenylhydrazone derivative (IV) is dissolved or suspended in an adequate solvent, for example, alcohol, ether, benzene or toluene. When alcohol is used, absolute alcohol achieves the good yield. Then, more than equivalent of dry hydrogen chloride gas is absorbed into the resultant solution or the suspension. Then the HCl salt of the N -acylated phenyl-hydrazine derivative (II) precipitates as crystals in good yield. Sulfuric acid or others can be used in place of gaseous hydrogen chloride. When ether, benzene or toluene is used as the solvent, a small quantity of alcohol should be added to it. The reaction temperature is preferably 0-25 C. though may be below 0 C.
As the N -acylated phenylhydrazone derivative (IV), various compounds can be illustrated. For example, the hydrazones of acetaldehye, chloral, benzaldehyde, acetal, ethyl acetoacetate and methoxy acetone can be easily decomposed in general cases to give the aimed N -acylated phenylhydrazine derivative (II). Among them, the hydrazone of acetaldehyde has especially distinctive commercial advantages.
According to the present invention, the following N acylated phenylhydrazine derivatives (II), for example, can be obtained:
N -cinnamoyl-N (p-methoxyphenyl) hydrazine N -cinnamoyl-N p-methylphenyl )hydrazine N -cinnamoyl-N (p-chlorophenyl) hydrazine N -cinnamoy1-N -(p-methylthiophenyl hydrazine N -cinnamoy1-N (p-ethoxyphenyl hydrazine N 5-phenyl-2,4-pentadienoyl) -N (:p-rnethoxyphenyl) hydrazine N (5 -phenyl-2',4'-p ent adienoyl) -N (p-ethoxyphenyl) hydrazine N -cinnamoyl-N (p-methoxyphenyl) hydrazine N -cinnarnoyl-N (p-methylphenyl hydrazine N 4-phenyl-3 -butenoyl) -N (p-methoxyphenyl) hydrazine N (m-nitrocinn amoyl -N m-methoxyphenyl) hydrazine N (rn-nitro cinnamoyl -N (p-methylphenyl hydrazine N (m-nitrocinnamoyl) -N (p-chlorophenyl) hydrazine N rn-nitrocinnamo yl) -N -(p-methylthiopheny1) hydrazine N (m-nitrocinnamoyl -N (p-ethoxyphenyl) hydrazine N (methylthio cinnamoyl) -N (p-methoxyphenyl) hydrazine N (p-methoxycinnamoyl) -N (p-methoxyphenyl) hydrazine N (p-chlorocinnamoyl -N -(p-methoXyphenyl hydrazine N -{5'- (p-ehlorophenyl -2',4'-pentadien0yl}-N (pmethoxyphenyl hydrazine N u-phenyl-p-chlorocinnamoyl) -N (p-methoxyphenyl) hydrazine N ,B-p-tolylacryloyl) -N (p-methoxyphenyl) hydrazine N u-methyl-p-chlorocinnamoyl -N (p-methoxyphenyl) hydrazine N (p-chlorocinnamoy1-N (m-methoxyphenyl hydrazine N -phenylacetyl-N (p-methoxyphenyl hydrazine N -phenylacetyl-N p-chlorophenyl) hydrazine N -phenylacetyl-N (p-methylthiophenyl hydrazine N -phenylacetyl-N (p-ethoxyphenyl )hydrazine 13 N -(p-methylphenylacetyl)-N -(p-methoxyphenyl) hydrazine N -phenylacetyl-N -phenylhydrazine N (3 '-pyridylacetyl) -N (p-methoxyphenyl hydrazine N 4'-pyridylacetyl -N p-methoxyphenyl) hydrazine N -(2-pyridylacetyl)-N -(p-methoxyphenyl)hydrazine N (2'-thienylacetyl -N (p-methoxyphenyl hydrazine N -(2-furylacetyl)-N -(p-methoxyphenyl)hydrazine N -(5'-chloro-2'-thienylacetyl)-N -(p-methoxyphenyl) hydrazine N -(diphenylacetyl)-N -(p-rnethoxyphenyl)hydrazine N -(2 phenyl-n-butyroyl)-N -(p-methoxypheny1) hydrazine N a-naphthylacetyl -N (p-methoxyphenyl) hydrazine N (fl-naphthylacetyl) -N (p-methoxyphenyl) hydrazine N -(m,p-dimethoxyphenylacetyl)-N -(p-methoxyphenyl) hydrazine N (4'-p-methoxyphenyl-n-butyroyl) -N (p-methoxyphenyl)hydrazine N 5 'phenyl-n-pentaoyl -N (p-methoxyphenyl) hydrazine N -(5'-phenyl-n-pentanoyl)-N -(p-ethoxyphenyl) hydrazi N -phenylacetyl-N -(m-methoxyphenyl)hydrazine N -pheny1acetyl-N (m-methylphenyl) hydrazine N (4'-phenyl-n-butyroyl -N (p-methoxyphenyl) hydrazine N -(fi-2-thienylacryloyl)-N -(p-methoxyphenyl) hydrazine N -fi-2'-thienylacryloy1) -N (p-methylphenyl) hydrazine N (B-2'-t hienylacryloyl -N (p-chlorophenyl) hydrazine N -(B-2'-thienylacryloyl)-N -(p-methylthiophenyl) hydrazine N -(5-2'-thienylacryloyl)-N -(p-ethoxyphenyl)hydrazine N (18-2'-thienylacryloyl) -N (m-methoxyphenyl) hydrazine N -(6-2'-furylacryloyl)N -(p-ethoxyphenyl(hydrazine N (B-Z-pyridylacryloyl) -N (m-methoxyphenyl) hydrazine N 3-2-pyridylacryloyl) -N (m-methylphenyl hydraz ne N -(B-2'-furylacryl0yl)-N -(p-methoxyphenyl)hydrazine N -{fl-(a'-naphthyl)-acryloyl}-N -(p-methoxyphenyl) hydrazine Their salts, for example, hydrochlorides, sulphates and phosphates, can be easily obtained. All of them are novel compounds that have not been reported in any literature.
These compounds have psychic, stimulating, anti-tumor, bactericidal and fungicidal activities and are very important as intermediates for producing strong antiinfiammatory drugs, analgesics and anti-pyretics.
In some cases the novel N -acylated phenylhydrazine derivatives (II) are directly obtained by reacting a phenylhydrazine derivative (X),
NHNH2 wherein R has the same meaning as identified in the Formula I, or salts thereof, with a compound (VI) wherein R and A have the same meanings as identified in the Formula I and Y is a halogen atom in this case, in the presence of a basic reagent.
This reaction is carried out in a conventional solvent such as benzene, toluene, xylene, ether, dioxane or tetrahydrofuran in the presence of a dehydrogenhalide agent such as a tertiary amine. As the tertiary amine, triethyl amine, pyridine or dimethylaniline is suitable and the amount thereof is required preferably at least equal mole of the said phenylhydrazine derivative (X).
This reaction proceeds so rapidly that the compound (V1) is added slowly to a phenylhydrazine derivative (X) in a suitable solvent while cooling. The N -acylated phenyl-hydrazine derivative (II) thus obtained is contaminated with a by-product such as N -acylated compound or N ,N -diacylated compound, however the objective N -acylated phenyl-hydrazine derivative (II) is separated and purified by removing theby-products by a suitable method such as column chloromatography. However, the purification of the N -acylated compound is not necessary, because only the N -acylated derivative is concerned with the following reaction in the present invention.
According to the process of the present invention, the compounds having the following substituents which are represented by R R and A in the said Formulas II, VI and X are obtained.
R phenyl, p chlorophenyl, p methylphenyl, p-methoxyphe-nyl, p-bromophenyl, p-ethylphenyl, p-ethoxy-- phenyl, 3-pyridyl, 4-pyridyl, Z-furyl, 3-furyl, Z-thienyl, S-thienyl, 5-chloro-2-thienyl, N-methyl-B-pyperidyl, anaphthyl, hydrogen atom;
R chlorine, bromine, fluorine, hydrogen, methyl, ethyl, isopropyl, methoxy, ethoxy, propioxy, methylthio, ethylthio and isopropylthio.
Lastly the process for producting a 1-acyl-3-indolyl aliphatic acid derivative (I) by the reaction of an N acylated phenylhydrazine derivative (11) w th an aliphatic acid derivative (III) will be described.
The reaction is carried out on heating in the presence of an adequate condensing agent or not in an organic solvent or not. The yield is very high.
This reaction is carried out on heating in the presence solvent but it is preferable to use a suitable solvent in many cases. As the solvent, organic acids, for example acetic acid, formic acid, propionic acid, lactic acid, butyric acid, non-polar organic solvents, for example cyclohexane, n-hexane, benzene, toluene, and other organic solvents, such as dioxane and dimethyl formamide are used in the ring formation reaction. When an alcohol is used as a solvent in this reaction, a corresponding ester of indole aliphatic acid is produced. For example,
CHsO- -NNHz-HC1 CHaC O CHzCHzC O OH CHsO- OHQCOOMO J CH3 NNH2 CHsCO CHzCHzC O OH CHaO N-NH2 CHaC O OHzCHzC O OH CHzCOOH CHzCOOH CHaO N CH; N CH3 These isomers can be generally separated by column chromatography.
At a temperature within a range of 50 to 200 C., the reaction generally proceeds but a temperature within a range of 65 to 95 C. is preferable. The reaction proceeds rapidly and is generally finished in a short period of reaction time, mostly in one or two hours. The condensing agent is not needed in some cases but desirable results are generally achieved by using a condensing agent. The condensing agent includes inorganic acids such as hydrochloric acid, sulfuric acid and phosphoric acid, metal halides such as zinc chloride and copper chloride, heavy metal powder such as copper powder, Grignards reagents, boron fluorides, polyphosphoric acid or ion-exchange resins. Hydrochloric acid or the like is required in an equimolar or larger amount, while copper powder or the like may be in a small amount,
In after treatment, the reaction mixture is allowed to stand at room temperature or in a refrigerator (about 5 C.), and then a large amount of crystals of the product is mostly obtained.
When crystals do not produce, the reaction mixture is concentrated under reduced pressure, or water, acetic acid- Water or petroleum ether is adequately added to the mixture. And then, the beautiful crystals can be obtained. Ether, acetone, acetone-water, alcohol, alcohol-water, benzene and acetic acid are generally preferred as a solvent for recrystallizing the present compound. Polymorphic crystals are often given in these compounds, and their crystal system varies with kind of a recrystallizing solvent and crystallizing velocity. The produced crystals are collected by filtration and they are generally washed with an aqueous solution of acetic acid, alcohol-water, water or petroleum ether before they are dried. Objective products are generally crystalline, but oily products are sometimes given in ester compounds.
Reaction solvents, reaction conditions, condensing agents and recrystallization solvents which have been mentioned above are only presented as illustrative of the present invention but not in its limitation, needless to speak of.
The following compounds are easily obtained in good yield, theoretically or in nearly theoretically, according to the process of the present invention:
1-cinnamoyl-2-methyl-5-methoxy-3-indolylacetic acid 1-cinnamoyl-5-methoxy-3-indolylacetic acid 1-cinnamoyl-2,5-dimethyl-3-indolylacetic acid 1-cinnamoyl-Z-methyl-S-chloro-3-indolylacetic acid l-cinnamoyl-2-methyl-3-indolylacetic acid 1-cinnamoyl-Z-methyl-S-methylthio-3-indolylacetic acid l-cinnamoyl-2-methyl-5-ethoxy-3-idolylacetic acid Dimethyl 1-cinnamoyl-2-methyl-S-methoxy-S-indolylmalonate 1-cinnamoyl-2-methyl-5-methoxy-3-indolylacetamide t-Butyl-l-cinnamoyl-Z-methyl-S-methoxy-3-indolylacetate Ethyl 1-cinnamoy1-2-methy1-5-methoXy-3-indo1ylacetate Methyl 1-cinnamoyl-2-methyl-5-methoxy-3-indolylacetate Benzyl 1-cinnamoyl-2-methyl-5-methoxy-3-indolylacetate Tetrahydropiranyl 1-cinnamoyl-2-methy1-5-methoxy-3- indolylacetate yl-cinnamoy1-2-methy1-5 methoxy-3-indolyl)butyric acid 00- 1-cinnamoy1-2-methyl-5-methyl-3-indolyl)propionic acid ,3- 1-cinnamoyl-Z-methyl-5-methoxy-3-indoly1)propionic acid 7- 1-cinnamoyl-2-methyl-5-methyl-3-indolyl propionic acid A mixture of 1-cinnamoyl-2-methyl-4-methoxy-3-indolylacetic acid and 1-cinnamoyl-2-methyl-6-methoxy-3- indolylacetic acid A mixture of 1-cinnamoyl-2,4-dimethyl-3-indoly1acetic acid and 1-cinnamoyl-2,6-dimethy1-3-indolylacetic acid A mixture of 1-cinnamoyl-4-methyl-3-indolylacetic acid and 1-cinnamoyl-6-methyl-3-indolylacetic acid 1- (4-phenyl-3 '-butenoy1) -2-methyl-5 -methoxy- 3-indolylacetic acid cc-{ 1- (4-phenyl-3 '-butenoyl) -2-methyl-5-methoxy- 3-indolyl}propionic acid 1- 2-a'-naphthylacryloyl) -2-methyl-5-methoxy- 3-indolylacetic acid I 1- (p-nitrocinnamoyl -2-methyl-5-methoxy- B-indolylacetic acid 1- ,8'-phenylcinnam oyl -2-methyl-5 -methoxy- 3-indolylacetic acid 1- (3 '-phenylcrotonoyl -2-methyl-5-methoxy- 3-indo1ylacetic acid 1- 2'-u'-pyridylacryloyl -2-methyl-5-methoxy- 3-indolylacetic acid 1-{2-(2"-quinolyl)-acryloyl}-2-methyl-5-methoxy- S-indolylacetic acid i 2-methyl-2-(a-naphthybpropionyl CH -(|3-O O- i Methyl-(4-phenyl)phenyl-acetyl (@Qonoo 2-ethyl-2-(a-naphthyl) butyroyl R C O OH 5 R COCH H mCH Rfl III-NH1 c 0 OH 11 (VIII) I (OH 01120 0 OH l \1II/\R2 wherein R R R R A and m have the same meanings as mentioned above.
The following compounds, for example, are easily ob- 5 Further in some cases an 1-acyl-3-indolylacetic acid derivative of the formula,
01120 O OH 22 wherein R R and A have the same meanings as identitied in the Formula I, is prepared from an N -acyl phenylhydrazine derivative (II) or salt thereof and acetosuccinic acid or 2-ketoadipic acid.
An example of this method is shown as follows:
ommQ-npvm-nor $0 CIH CH3OOCHCH2COOH ([3112 00011 or (|)HCOCH2CH2COOII COOH CHaO- "*1 (311200011 l The following compounds, for example, are prepared in a good yield by this method.
1-cinnamoyl-2-methyl-5-methoxy-3-indolylacetic acid 1-(p-methoxycinnamoyl)-2-methy1-5-methoxy-3-indolylacetic acid 1-phenylacetyl-2-methyl-S-methoxy-3-indo1ylacetic acid 1-cinnamoyl-Z-methyl-S-ethoxy-3-indolylacetic acid 1-cinnamoyl-2-methyl-3-indoly1acetic acid 1-cinnamoyl-2-methyl-5-methoxy-3-indolylacetic acid 1- (B-Z-pyridylacryloyl) -2-methyl-5-methoxy-3 -indoly1- acetic acid Further, in the process of the present invention, a 1- acyl-3-indolyl aliphatic acid derivative (1) can be obtained directly from an N -acylated hydrazone derivative (IV) in some cases, in which a 1-acyl-3-indolyl aliphatic acid derivative (I) is produced by reacting an N -acylated phenyl hydrazone derivative (IV) with an aliphatic acid derivative (IH) on heating in the presence of a suitable condensing agent or not in an organic solvent or not.
According to this method, as a solvent, organic acids such as formic acid, propionic acid, lactic acid and butyric acid, non-polar organic solvents such as cyclohexane, nhexane, benzene and toluene or an alcohol may be available.
The reaction generally proceeds at 50 to 200 C., preferably at 65 to C.
In organic acids such as hydrochloric acid and sulfuric acid, metal halides such as Zinc chloride and copper chloride, boron fluoride and polyphosphoric acid can be used as a condensing agent in the reaction.
The following 1-acyl-3-indolyl aliphatic acid derivatives, for example, can be prepared by the method,
1-phenylacetyl-2-methyl-5-methoxy-3-indolylacetic acid 1-cinnamoyl-Z-methyl-S-methoxy-3-indolylacetic acid Methyl 1-phenylacetyl-2-methyl-5-methoxy-3-indolylacetate Ethyl 1-phenylacetyl-Z-methyI-S-methoxy-3-indolylacetate Tertiary butyl 1-pheny1acetyl-Z-methyl-S-methoxy-3- indolylacetate In this case when R is a carboxy group in the Formula 5 III, the carboxy group is decarbonated to yield an 1- acyl-3-indolyl aliphatic acid derivative (VII), as shown below.
R (1 1 ooon -NN=B RCOCH2- on .,.---on 0 00011 A VH1 1'1 R o o it in v11) Following compounds, for example, are obtained by this method.
1-phenylacetyl-2-methyl-S-methoxy-3-indolylacetic acid 1-cinnamoyl-Z-methyl-S-methoxy-3-indolylacetic acid Still further, according to the present invention a 1- acyl-3-indolyl aliphatic acid derivative represented by the Formula I is prepared from the phenylhydrazine derivative of the Formula IX or a salt thereof and an aliphatic acid derivative of the Formula III in the presence or absence of a suitable solvent, and in the presence or absence of a condensing agent.
In the reaction, suitable solvents are organic acids, such as acetic acid, formic acid, propionic acid and lactic acid, non-polar solvents such as cyclohexane, n-hexane, benzene, toluene and xylene, ether compounds such as dioxane and diisopropyl ether or other conventional organic solvents. Suitable condensing agents are inorganic acids such as hydrochloric acid, sulfuric acid, phosphoric acid and hydrogen chloride, metal halides such as zinc chloride, copper chloride, a metal powder, such as copper powder, a Grignards reagent, a boron fluoride, polyphosphoric acid or an ion-exchanger resin. However, it is not always necessary to use a solvent or a condensing agent. This 'indole ring formation proceeds under heating the reaction mixture at 50 to 200 C., and is completed within several hours. Preferably, the reaction is continued at 65 to 95 C. for 1 to 4 hours.
After completion of the reaction, the reaction mixture is allowed to cool, a large amount of crystals are generally produced as precipitate. Even in the case which crystals are not produced, if the solvent is removed by dis- 70 tillation or water or petroleum ether is added to the reaction mixture, a large amount of crystals can be obtained. The crude crystals are collected by filtration, washed with water and thereafter recrystallized from a suitable solvent to give a pure objective product.
24 This process of the present invention, for example, is shown by a chemical equation as follows:
in CHaCOOH CHaO-Q-III-NH: CHaCOCHzCHzCOOH C=NH (H01) a, in
CHaO C H20 0 011 N CH:
For example, l-acyl-3-indolyl aliphatic acid derivatives which are prepared by this method are as follows, but it is not necessary to say that 1-acyl-3-indolyl aliphatic acid derivatives are not restricted only to them.
Still further, according to the present invention a 1-acyl-3-indolyl aliphatic acid derivative (XII) is obtained by converting an ester or amide derivative (XIII) of a corresponding 3-indo1yl aliphatic acid.
For example, a benzyl ester of a 3-indolyl aliphatic acid is converted to a free 3-indoyl aliphatic acid derivative (XII) by hydrogenating with decomposition in the presence of a metal catalyst such as palladium.
This method is shown, for example, as following reaction formula:
cmo omoooom- 01130 omcoon \N CH3 If the alcohol moiety of the ester compound (X) is tertiary butyl alcohol, the ester is treated with an arylsulfonic acid such as p-toluenesulfonic acid to yield an objective product.
If a tertiary butyl ester is only fused by heating, it is decomposed to yield an objective free 3-indolyl aliphatic acid derivative (XH).
In a few cases a free 3-indolyl aliphatic acid derivative may be obtained by treating an amide of the corresponding 3-indo1yl aliphatic acid derivative with a suitable amount of nitrous acid in an inert solvent.
The following compounds, for example, are prepared by this method.
1-cinnamoyl-2-methyl-5-methoxy-3-indolylacetic acid 1-phenylacetyl-2-methyl-5-methoxy-3-indolylacetic acid 1- (p-tolylacetyl)-2-methyl-5-methoxy-3-indoly1acetic acid 1- (p-isobutylphenylacetyl) -2-methyl-5 methoxy-3 -indolylacetic acid 1-phenylacetyl-2-methyl-5-chloro-3-indolylacetic acid l-phenylacetyl-2,5-dimethyl-3-indolylacetic acid l-phenylacetyl-2-methyl-5-rnethylthio-3-indolylacetic acid fl- 1-phenylacetyl-2-methyl-5 -methoxy-3 -indolyl) propionic acid ou( 1-phenylacetyl-2-methyl-5 -methoxy-3 -indoly1) propionic acid 1- (m-chlorophenylacetyl) -2-methy1-5 -methoxy-3 -indoly1- acetic acid l-phenylacetyl-5-methoxy-3-indolylacetic acid 1-phenylacetyl-2-methyl-4-methoxy-3-indolylacetic acid 1-phenylacetyl-2-methyl-6-methoxy-3-indolylacetic acid l-(a-naphthylacetyl)-2-methyl-5-methoxy-3-indolylacetic acid l-{fi-Z-furyl) -acryloyl} -2-methyl-5-methoxy-3- indolylacetic acid 1- 2'-thienylacetyl) -2-methyl-5 -metl1oXy-3 -indolylacetic acid 1- {2'- (a-pyridyl) -acrylol}-2-methy1-5 -methoxy-3- indolylacetic acid 26 1-(2'-phenylbutyroyl)-2-methyl-5-methoxy-3- indolylacetic acid 1-(2'-pheny-lbutyroy1)-2,S-dimethyl-3-indolylacetic acid 1- 3 -phenylp ropionoyl) -2-methyl-5-methoxy-3- indolylacetic acid 1- 3 '-phenylpropionoyl) -2-methyl-5-chloro-3- indolylacetic acid 1-pivaloyl-2-methyl-5-methoxy-3-indo1ylacetic acid Still further, according to the present invention, a lacyl-3-indolyl aliphatic acid derivative represented by the Formula XV can be prepared by oxidizing an indolyl-3- acetaldehyde derivative represented by the Formula XVI.
In this process of the present invention, potassium permanganate, hydrogen peroxide, organic peroxide, silveroxide, selenium dioxide and copper hydroxide can be used as an oxidizing agent, and especially potassium permanganate or copper hydroxide is most preferable in many cases.
The following groups, for example, are given as the substituents shown by -AR R and R of the l-acy 3-indolyl aliphatic acid derivatives of the said Formula XV which are easily prepared by this method.
A-R benzyl, styryl, cinnamyl, 2-phenylvinyl, 2-(2'- thienyl)-vinyl, 2(3'-pyridyl)-vinyl, 2 (2' furanyl)- vinyl, p-methoxybenzyl, p-methylbenzyl, 2,2-diphenylvinyl, 2-(cinnamyl)-vinyl, N-methyl-hexahydro-3-pyridylmethyl, 2 (4' pyridyl) vinyl and 2-(5'-chloro-2'- thienyl)-vinyl.
R and R hydrogen, methyl and ethyl.
R methoxy, ethoxy, iso-propyloxy, methyl, ethyl, n-
propyl, isopropyl, tertiary-butyl, methylthio, ethylthio and hydrogen.
An indole-3-acetaldehyde derivative (XVI), which is a starting substance of this method, can be prepared according to the following reaction process:
27 phenylacetyl)-2-methyl-5-methoxy 3 indolyl-acetaldehyde as shown by the following reaction formula:
Still further, according to the present invention, a 1- acyl-3-indolylacetic acid derivative of the Formula XV is prepared by oxidizing the corresponding indole-3-ethanol derivative of the Formula XIX.
That is, an indole-3-ethanol derivative(XIX) is oxidized by potassium permanganate or chromic acid to give a 1-acyl-3-indolylacetic acid derivative (XV) in a good yield.
Substituent represented by R R R R and A of a 1-acyl-3-indoly1 acetic acid derivative (XV) which can be prepared by this method, are as follows:
-A-R benzyl, styryl, cinnamyl, 2-(2'-thienyl)-viny1,
2- 3 '-pyridy1)-vinyl, 2-(2-furanyl) -viny1, p-methoxybenzyl, p-methylbenzyl, 2,2-diphenylvinyl, Z-(cinnamyl)-vinyl, N-methylhexahydro-3-pyridylmethyl, 2-(4'- pyridyl)-viny1 and 2-(5 '-chloro-2-thieny1)-vinyl.
---R and -R hydrogen, methyl and ethyl.
-R methoxy, ethoxy, iso-propyloxy, methyl, ethyl, n-
propyl, iso-propyl, tertiary-butyl, methylthio, ethylthio and hydrogen.
28 The indole-3-ethanol derivative (XIX), a starting compound of this method, is prepared by following reaction process:
wherein R R R R and A have the same meanings as identified above.
For example, 2-(1-cinnamoyl 2' methyl-5'-methoxy- 3' indo1y1)-ethanol is obtained in high yield according to the following reaction process:
Still further, according to the present invention, a l-acyl 3 indolylacetic acid derivative (XV) is prepared by dehydrogenation of the corresponding 2,3-dihydro-3-indolylacetic acid derivative (XXI).
In this dehydrogenation, non-polar solvent, such as benzene, xylene and toluene and other various organic solvents, such as acetone, acetic acid, chloroform, ethanol and methanol can be used.
As agents for the dehydrogenation, chloranil, selenium dioxide, halogen and the like oxidizing agents can be used.
According to the above method, the following compounds can be easily obtained:
1-cinnamoyl-2-methyl-5-methoxy-3-indolylacetic acid 1- p-chloro cinnamoyl -2-methyl-5-methoXy-3- indolylacetic acid 1- cinnamoylacryloyl) -2-methyl-5-methoxy-3 indolylacetic acid 1-(p-methoxyphenylacetyl)-2-methy1-5-methoxy-3- indolylacetic acid 1- (p-tolylacetyl) -2-methyl-5-methoxy-3 -indolylacetic acid A starting substance, l-acyl 2,3 dihydro-3-indolyl aliphatic acid derivative (XXI) is obtained in a high yield by reacting a 2,3-dihydro 3 indolyl aliphatic acid derivative with a corresponding acyl chloride in the presence of a hydrogen chloride acceptor.
Still further, according to the present invention a 1- acyl-3-indolylacetic acid derivative of the Formula XXII is prepared by dehydration of a Z-hydro 3 hydroxy- 3-indolylacetic acid derivative of the Formula XXIII and then, if necessary, by hydrolysis of the rwultant ester compound.
The reaction proceeds at a temperature within the range of 70 to 200 C. However, if a reaction does not proceed smoothly, the compound (XXIII) is azeotropically refluxed with an azeotropic solvent, for example benzene, toluene or xylene, or is heated in the presence of a suitable dehydrating agent, for example a proper amount of anhydrous sodium sulfate, whereby a dehydration reaction takes place.
When a 1 acyl 3 indolylacetic acid derivative (XXII), in which R is an alkyl group, for example t-butyl group, is treated in the presence of arylsulfonicacid, it is converted without affecting the acid-amide bonding to the desired free acid.
The 2-hydro 3 hydroxy 3 indolylacetic acid derivative :(XXIII), the starting compound of the above process, is prepared by heating the mixture of alkyl halogenoacetate and a corresponding indole derivative with stirring in a non-polar organic solvent in the presence of zinc powder and, if necessary, a small piece of iodine. One example of the above reaction is as follows:
CHaO- BICH2COO$BI1 cum-@j HzOOOt-Bu The following compounds, for example, are prepared by this method.
1- naphthylacetyl) -2-methyl-5 -methoxy-3 -indolyl acetic acid.
Still further, according to the present invention, a crude l-acyl 3 indolylacetic acid derivative (XV) is obtained by heating an 'y-(N-acyl-anilino)-aliphatic acid derivative (XXIV) in the presence of a suitable catalyst or a dehydrating agent. The crude product thus obtained can be purified by recrystallization from a proper organic solvent or, if the recrystallization fails, a columnchromatography can be used.
3-indolylacetic acid derivatives having the following groups as R R R R and A in the Formula XV are easily obtained by this method.
AR benzyl, styryl, cinnamyl, 2-(2'-thienyl)-vinyl,
2-(3'-pyridyl) -vinyl, 2-(2'-furanyl)-vinyl, 2- (cinnamyl)-vinyl, N-methyl-hexahydro-3-pyridy1- methyl, 2-(4'-pyridyl)-vinyl, 2,2-diphenylvinyl and 2-(3'-chloro-2'-thienyl)-vinyl.
R and R hydrogen, methyl and ethyl.
R methoxy, ethoxy, iso-propyloxy, methyl, ethyl,
n-propyl, iso-propyl, tertiary butyl, methylthio, ethylthio and hydrogen.
The y (N acyl-anilino)-aliphatic acid derivative (XXIV), which is a starting substance of this method, is generally prepared by following processes:
(XXIV) wherein R R R R R and A have the same meanings as identified above. For example, the reaction of p-anisidine with cinnamoyl chloride gives N-cinnamoylanisidine, which is subjected to a reaction with tertiary butyl-'ybromo-B-keto-valeriate to yield tertiary butyl 4 {N (pmethoxyphenyl) N (phenylacetyl)}amino 3 oxovaleriate. And then the resulting tertiary butyl-4-{N-(pmethoxyphenyl) N (phenylacetyl)}amino 3 oxovaleriate is hydrolyzed to give an oily substance of 4-{N- (p-methoxyphenyl) N (phenylacetyl)}amino 3 oxovaleric acid.
Still further, according to the present invention, a 1-acyl-3-indolylacetic acid compound (IX) is prepared from a 3-(2'-acylaminophenyl)-levulinic acid derivative represented by the Formula XXV in the presence of inorganic acid in a suitable solvent.
A range of reaction temperatures is from 40 to 120 C., and the most desirable result is obtained at temperature within the range of 60 to C.
After a completion of reaction, a reaction mixture is neutralized, concentrated, and extracted with a suitable organic solvent, and then a separated organic layer is dried and concentrated to a crude crystalline substance.
31 Recrystallization from ether, acetone or acetone-water gives a pure product.
Among novel 3-indolyl aliphatic acid derivatives of the present invention, there are not only a few useful compounds, which indicate excellent anti-inflammatory action but also possess extremely low toxicity.
In contrast to the above facts, the many compounds of this invention are markedly low in toxicity, and even when over 1,000 mg./kg. of these compounds are orally administered to each of rat and mouse, they scarcely 32 yield 20 g. acetaldehyde N -(phenylacetyl)-N-(p methoxyphenyDhydrazone, M.P. 9810l C. Y
In a way similar to that in Example 1,-the following hydrozones were obtained.
EXAMPLE 2 Acetaldehyde N -(B phenylpropionyl) N methoxyphenyl)-hydrazone, M.P. 134-135 C.
EXAMPLE 3' Acetaldehyde N p chlorophenylacetyl) N (1 methoxyphenyl)hydrazo'ne, M.P. 93-96 C. w
EXAMPLE 4' e Acetaldehyde N (p methoxyphenyl) EXAMPLE Acetaldehyde. N (a chlorophenylacetyh N l-(pmethoxyphenyl)hydrazone, M.P. 107-11 0 C. ;i
TABLE Efiects 50% lethal 50% dose of rat, Therainhibiting per os peutic Compound dose 1 (mg./kg.) ratio 2 l-(p-chlorobenzoyl)-2-methyl-5-methoxy-3- indolylacetic acid (Indomethacin) 7. 5 2. 0 1,2-dipl1enyl-3,5-dioxo-4-n-butylpyrazol (Phenylbutazone) 320 ca. 700 ca. 2. 2 1-(2-Furylacry1oyl)-2-methyl-5-methoily-3- indolylacetic acid resent compound) 1,000 40. 0 l-(B-phenylpropionyl -2-methyl-5-methoxy-3- indolylacetic acid (present compound) 250 1, 500 6. 0 Ethyl l-cinnamoyl-2-methyl-5-meth OXy-3 indolylacetate (present compound) 65 1, 500 23. 1 l-phenylacetyl-2-methyl-5-methoxy-3-indolylacetic acid (present compound) 210 1, 500 7. 1 1-cinnamoyl2-methyl-fi-methoxy-3-indolylacetic acid (present compound) 12 1, 500 125 1-(2-thienylacryloyl)-2-methyl-5-methoxyindolylacetic acid (present compound) 18 1, 200 67. 0 a-[l-cinnamoyl-2-methyl-5-methoxy-3-indolyl]- propionic acid (present compound) 20 1, 500 75. 0 l-(a-methylcinnamoyl)-2-methyl-5-methoxy-3- indolylacetic acid (present compound) ca. 1, 500 37. 5
4 Of Oarrlmeedz'n edema of rats hind paw, per os (mg/kg).
2 60% lethal dose/% inhibiting dose of Carrageenin edema.
The present inventors prepared many other 1-acyl-3- indolyl aliphatic acid derivatives than the compounds shown in the aforesaid table and evaluated the pharmaceutical effects thereof by animal tests.
The present inventors have found that many derivatives (I) of 1-acyl-3-indolyl aliphatic acid, which are prepared by the present invention, are superior to l-(pchlorobenzoyl) 2 methyl 5 methoxy 3 indolylacetic acid (Indomethacin) and 1,2-diphenyl-3,5-dioxo-4- n-butylpyrazolidine (Phenylbutazone) in the therapeutic ratios thereof and has a great practical value.
It has found that these compounds also have comparatively potent analgesic activities shown by Haffners meth- 0d, and antipyretic activities in a pyrogen test.
DESCRIPTION OF THE PREFERRED EMBODIMENTS The following examples are given to illustrate the present invention more particularly but it is not intended to limit the present invention only to them.
EXAMPLE 1 13.8 g. of phenylacetyl chloride was dropped to 13.1 g. of acetaldehyde N -(p-rnethoxyphenyl)hydrazone in 50 ml. of pyridine under cooling with ice. Thereafter stirring of the reaction mixture was continued for additional overnight under cooling with ice, and then it was poured into 250 ml. of cold water. As a result, a large amount of crystals were produced. The resultant crystals were collected by filtration, washed with water, and dried to EXAMPLE 6 Acetaldehyde N (a naphthylacetyl) N p methoxyphenyl)hydrazone, M.P. -103 C.
EXAMPLE 7 Acetaldehyde N (m,p dimethoxyphenylacetyl)-N (p-methoxyphenyl)hydrazone, M.P. 8890C.
EXAMPLE 8 Acetaldehyde N cinnamoyl N (p methoxyphenyl)hydrazone, M.P. 166170 C.
EXAMPLE 9 Acetaldehyde N -(a-methylcinnamoyl) N (p methoxyphenyDhydrazone, MP 1 14-1 15 C.
EXAMPLE 10 EXAMPLE 11 Acetaldehyde N (p methoxycinnamoyl) -N -'(p-methoxyphenyl)hydrazone, M.P. 172179 C.
EXAMPLE 12 Acetaldehyde N (p tolylacryloyl)-N -(p-meth xyphenyl)hydrazone, M.P. 169172 C.
EXAMPLE 13 f Acetaldehyde N -(m-nitrocinnanioyD-N (p-methoxyphenyl)hydrazone, M.P. 170180 C.
33 EXAMPLE 14 Acetaldehyde N (B-2'-furylacryloyl)-N -(p-methoxyphenyl)hydrazone, M.P. 143 -146' C.
EXAMPLE 15 EXAMPLE 16 N (m-nitrocinnamoyl)-N -(p-methoxyphenyl)hydrazine hydrochloride, M.P. 165 l70 C. (decomp.).
EXAMPLE 17 N cinnamoyl N -p-tolyl)hydrazine hydrochloride, M.P. 173-l75 C. (decomp.).
EXAMPLE 18 N (a phenylcinnamoyl)-N -(p-methoxyphenyl)hydrazine hydrochloride, M.P. 145 -160 C. (decomp.).
EXAMPLE 19 N (,8 p-tolylacryloyl)-N -(p-methoxyphenyl)hydrazine hydrochloride, M.P. 187 C. (decomp.).
EXAMPLE 20 N (c methylcinnamoyl)-N -(p-methoxyphenyl)hydrazine hydrochloride, M.P. 174 C. (decomp.).
EXAMPLE 21 N -(p chlorocinnamoyl) N (p-methoxyphenyl)hydrazine hydrochloride, M.P. 179-182 C. (decomp.).
EXAMPLE 22 N (p-methoxycinnamoyl)N -(p-methoxyphenyl)hydrazine hydrochloride, M.P. 178 C. (decomp.).
. EXAMPLE 23 N (a methyl m nitrocinnamoyl)-N -(p-methoxyphenyl)hydrazine hydrochloride, M.P. l68-171 C. (decomp.).
EXAMPLE 24- N (phenylacetyl) N -(p-methoxyphenyl)hydrazine hydrochloride, M.P. 165-166 C. (decomp.).
EXAMPLE 25 N (B phenylpropionyl)-N -(p-methoxyphenyl)hydrazine hydrochloride, M.P. 179 C. (decomp.).
EXAMPLE 26 N (p' chlorophenylacetyl)-N -(p-methoxyphenyl) hydrazine hydrochloride, M.P. 202203 C. (decomp.).
EXAMPLE 27 N {'y (p'-methoxyphenyl)-n-butyroyl}-N -(p-methoxyphenyl)hydrazine hydrochloride, M.P. 166 C. (decomp.).
EXAMPLE 28 N (a chlorophenylacetyl) -N -(p-methoxyphenyl) hydrazine hydrochloride, M.P. 130 C. (decomp.).
EXAMPLE 29 N (diphenylacetyl) N (p-methoxyphenyl)hydrazine hydrochloride, M.P. 144l46 C. (decomp.).
EXAMPLE 30 N (p' nitrophenylacetyl)-N -(p-methoxyphenyl)hydrazine hydrochloride, M.P. 204-205 C. (decomp.).
34 EXAMPLE 31 N (a naphthylacetyl)-N -(p-methoxyphenyl)hydrazine hydrochloride, M.P. 173 C. (decomp.).
EXAMPLE 32 N -pheny1acety1-N -(p-methylphenyl)hydrazine hydrochloride, M.P. l5l-l52 C. (decomp.).
EXAMPLE 33 N -(phenylacetyl)-N -phenylhydrazine hydrochloride, M.P. l49 C. (decomp.).
EXAMPLE 34 N (phenylacetyl)-N (p-chlorophenyl)hydrazine hydrochloride, M.P. 167 C. (decomp.).
EXAMPLE 35 N (m,p dimethoxyphenylacetyl) N (p-methoxyphenyl)hydrazine hydrochloride, M.P. 165 C. (decomp.).
EXAMPLE 36 58 g. of acetaldehyde N -(B-T-furylacryloyl)-N -(pmethoxyphenyl)hydrazone was suspended in 400 m1. of ethanol, and the suspension mixture was shaken under cooling with ice.
30 g. of gaseous hydrogen chloride was absorbed in the reaction mixture over one hour.
The produced crystals were filtered, washed with 100 ml. of ether and dried to give 35.0 g. of N -(,8-2-furylacryloyl -N p-methoxyphenyl) hydrazine hydrochloride; M.P. 166 c. (decomp.).
In a way similar to this, N -(B-Z-thienylacryloyl)-N (p-methoxyphenyl)hydrazine hydrochloride was obtained.
EXAMPLE 37 7.7 g. of phenylacetyl chloride was dropwise added to the solution mixture of 6.1 g. of p-tolylhydrazine and 5 g. of triethylamine in ml. of toluene under cooling with ice.
Thereafter the temperature of the reaction mixture was raised slowly up to 7075 C. and the heating of the mixture was continued at 7075 C. for an additional 20 minutes. After the reaction mixture was allowed to cool, the crystals produced were removed by filtration, and the filtrate was concentrated to oily residue, which was recrystallized from a solution mixture of ethanol and water to give N -phenylacetyl-p-tolylhydrazine, M.P. 86-87 C. It was treated with ethanolic hydrogen chloride to give N -phenylacetyl-p-tolylhydrazine hydrochlo ride; M.P. 15l152 C. (decomp.).
EXAMPLE 38 6.0 g. of cinnamoyl chloride was dropwise added to a mixture of 8.7 g. of p-methoxyphenylhydrazine hydrochloride, 10.1 g. of triethylamine and 200 ml. of toluene under cooling at 5 to 0 C.
The reaction mixture was stirred at 20 25 C. for one hour. The separated precipitates were filtered off and dry gaseous hydrogen chloride was introduced into the filtrate. As a result a large amount of crystals of N -cinnamoyl- N -(p-methoxyphenyl)hydrazine hydrochloride were produced. These crystals were collected by filtration and washed with 20 ml. of ether and dried to yield N cinnamoyl-N (p-methoxyphenyl)hydrazine hydrochloride of M.P. 183 -185 C.
The following compounds were prepared in a way similar to that in Example 38.
EXAMPLE 39 N (phenylacetyl -N (p-methoxyphenyl) hydrazine hydrochloride, M.P. l66-l67 C. (decomp.).
EXAMPLE 40 N cinnamoyl-N -(p-methoxyphenyl)hydrazine hydrochloride, M.P. 179 C. (decomp.).
35 EXAMPLE 41 N -(p'-methoxycinnamoyl)-N -(p-methoxyphenyl) hydrazine hydrochloride, M.P. 178C. (decornp.)
EXAMPLE 42 N (p-chlorophenylacetyl)-N (p-methoxyphenyl) hydrazine hydrochloride, M.P. 202 C. (decomp).
EXAMPLE 43 N (fi-2-furylacryloyl)-N (p methoxyphenyl)hydrazine hydrochloride, M.P. 166 C. (decomp.).
EXAMPLE 44 N (ot-chlorophenylacetyl) N (p-methoxyphenyl)hydrazine hydrochloride, M.P. 123 C. (decomp.).
A mixture of g. of N -(phenylacetimidoyl)-N -(pmethoxyphenyl)hydrazine hydrochloride, 5 g. of levulinic acid and 30 ml. of acetic acid was heated at 80"-85 C. for 3 hours under vigorous stirring.
After the reaction mixture was allowed to cool 50 ml. of water was added thereto to give crystals. They were collected by filtration, washed with 50 ml. of water and recrystallized from a mixture of acetone and water to give 1 phenylacetyl-Z-methyl-5-methoxy-3-indolylacetic acid, M.P. 138139 C.
The following compounds were prepared according to a method similar to that of Example 49.
EXAMPLE 50 1 cinnamoyl-Z-methyl-5-methoxy-3-indolylacetic acid, M.P. l64-165 C.
EXAMPLE 51 'y-(l cinnamoyl-Z-methyl-5-methoxy-3-indo1yl)butyric acid, M.P. 125 -126 C.
EXAMPLE 52 5 (1 cinnamoyl 2-methyl-5-methoxy-3-indolyl)propionic acid, M.P. 189l90 C.
EXAMPLE 53 l-(fl-Z-furylacryloyl)-2-methyl 5 methoxy-3-indolylactic acid, M.P. 163 -164 C.
EXAMPLE 54 1 (p methoxycinnamoyl)-2-methyl-5-methoxy-3-indolylacetic acid, M.P. 193-l95 C.
EXAMPLE 55 1- (p-chlorophenylacetyl) 2 methyl 5 methoxy-3-indolylacetic acid, M.P. l80-l82 C.
EXAMPLE 56 Ethyl l-cinnamoyl-Z-methyl-5-methoxy-3-indolylacetate, M.P. 162163 C.
EXAMPLE 57 1 (u phenylbutyroyl) 2-methyl-5-methoxy-3-indolylacetic acid, M.P. 123125 C.
36 EXAMPLE 5s 1 (3',4 dimethoxyphenyl)-2-methyl-5-methoxy-3- indolylacetic acid, M.P. 169 170 C.
EXAMPLE 59 1 (a. chlorophenylacetyl)-2-methyl-'5-methoxy3- indolylacetic acid, M.P. 165 166 C.
EXAMPLE 60 20 g. of N (phenylacetyl)-N -(p-methoxypl1enyl)- hydrazine hydrochloride was added to 30 g. of levulinic acid. The mixture was heated at 76 C. for 3 hours, and then allowed to stand at room temperature overnight. The precipitates were collected by filtration, washed with water and dried to give crude crystals of 1-(phenylacetyl)- Z-methyl-5-methoxy-3-indolylacetic acid, which was recrystallized from acetone-water to give 9.7 g. of a purified product, M.P. 14l.5l43 C.
Microanalysis.-Calculated (percent): C, 71.20; H, 5.68; N, 4.15. Found (percent): C, 71.54; H, 5.84; N, 3.96.
According to this method, l-(phenylacetyl)-2-methyl- 5-methoxy-3-indoly1acetic acid was prepared in a similar yield from a mixture of 10 g. of levulinic acid and 25 ml. of acetic acid instead of 30 g. of levulinic acid.
EXAMPLE 61 A mixture of 10 g. of N -(diphenylacetyl)-N -(pmethoxyphenyDhydrazine hydrochloride and 20 g. of levulinic acid was heated at 75 83 C. for 2.5 hours with stirring. After the completion of reaction, the reaction mixture was poured into water, and then precipitate was produced. It was collected by filtration, Washed with water and recrystallized from acetone-water to give light gray crystals of 1-diphenylacetyl-Z-methyl-S-methoxy-3- indolylacetic acid; M.P. l5l C.
Microanalysis.-Calculated (percent): C, 75.53; H, 5.61; N, 3.39, Found (percent): C, 74.92; H, 5.56; N, 3.69.
EXAMPLE 62 In a way similar to that of Example 61, l-(u-chlorophenylacetyl) 2-methyl-5-methoxy-3-indolylacetic acid, M.P. l66 C., was prepared from N (0c-Ch10IO- phenylacetyl) N -(p-methoxyphenyl)hydrazine hydrochloride and levulinic acid.
Microanalysis.-Calcnlated (percent): C, 64.18; H, 4.88; N, 3.77; Cl, 9.54. Found (percent): C, 63.70; H, 4.89; N, 3.86; or, 9.77.
EXAMPLE 63 In a way similar to that in Example 61 l-(a-phenylbutyroyl)-2-methyl-5-methoxy-3-indolylacetic acid, M.P. 123.5 -125 C. was prepared from N -(u-phenylbutyroyl) -N (p-methoxyphenyl) hydrazine hydrochloride and levulinic acid.
Microanalysis.-Calculated (percent): C, 72.33; H, 6.30; N, 3.84. Found (percent): C, 72.51; H, 6.38; N, 3.94.
EXAMPLE 64 In a way similar to that in Example 61, l-(ot-naphthylacetyl) 2-methyl-5-methoxy-3-indolylacetic acid, M.P. 165.5-166.5 C, was prepared from N -(a-naphthylacetyl) N (p-methoxyphenynhydrazine hydrochloride and levulinic acid.
Microanalysis.Ca1culated (percent): C, 74.39; H, 5.42; N, 3.62. Found (percent): C, 74.43; H, 5.54; N, 3.70.
EXAMPLE 65 A mixture of 10 g, of N -(diphenylacetyl)-N -(pmethoxyphenyl)hydrazine hydrochloride and 20 g. of ethyl levulinate was heated in 20 ml. of ethanol at 80- 83 C. for 6.5 hours with stirring. After completion of the reaction, the mixture was allowed to cool and poured into Water to give a dark blue homogenous solution,
37 which was extracted with ether, and then the ether layer was dried on anhydrous sodium sulfate and concentrated to give light yellow crystals. The recrystallization from ether-alcohol gave pure pale yellow crystals of ethyl 1- diphenylacetyl 2 methyl-5-methoxy-3-indolylacetate, M.P. 121122 C.
Microanalysis.-Calculated (percent): C, 76.19; H, 6.12; N, 3.17. Found (percent): C, 75.59; H, 6.21; N, 3.13.
EXAMPLE 66 In a way similar to that in Example 61, l-(p-chlorophenylacetyl) 2-methyl-5-methoxy-3-indolylacetic acid, M.P. 178 C., was prepared from g. of N -(p-chlorophenylacetyl) N -(p-methoxyphenyl)hydrazine hydrochloride and 30 ml. of levulinic acid.
Microanalysis.Calculated (percent): C, 64.60; H, 4.89; N, 3.77. Found (percent): C, 64.45; H, 4.96; N, 3.86.
EXAMPLE 67 5 g. of N (p-nitrophenylacetyl)-N -(p-methoxyphenyl)hydrazine hydrochloride and 10 g. of levulinic acid were added to 30 ml. of acetic acid. The mixture was heated at 9095 C. for 2 hours under stirring. Thereafter the mixture was poured into 150 ml. of water, and the resultant precipitates were collected by filtration, washed with water and recrystallized from dioxane to give 1 (p-nitrophenylacetyl)-2-methyl-5-methoxy-3-indolylacetic acid, M.P. 207 C.
Microanalysis.Calculated (percent): C, 62.82; H, 4.75; N, 7.33. Found (percent): C, 63.11; H, 4.77; N, 7.26.
EXAMPLE 68 In a way similar to that in Example 61, 1('y-p-methoxyphenyl-n-butyroyl) 2 methyl-5-methoxy-3-indolyl acetic acid, M.P. 163 C., was prepared from N -(v-pmethoxyphenyl-n-butyroyl) N (p-methoxypheny1)- hydrazine sulfate and levulinic acid.
EXAMPLE 69 In a way similar to that in Example 61, 1-m,p-dimethoxyphenylacetyl) 2 methyl-S-methoxy-3-indolylacetic acid, M.P. 169-170 C., was prepared from N -(m,p-dimethoxyphenylacetyl) N -(p-methoxyphenyl)hydrazine hydrochloride and levulinic acid.
Microanalysis.--Calculated (percent): C, 66.49; H, 5.83; N, 3.55. Found (percent): C, 66.46; H, 5.93; N, 3.41.
EXAMPLE 70 In a way similar to that in Example 61, 1-(,8-phenyl-npropionyl)-2-methy1-5-methoxy-3-indolylacetic acid, M.P. 163164 C., was prepared from N -(fi-phenyl-n-propionyl) N -(p-methoxyphenyl)hydrazine hydrochloride and levulinic acid.
Microanalysis.Calculated (percent): C, 71.78; H, 6.02; N, 3.98. Found (percent): C, 71.85; H, 6.07; N, 4.08.
EXAMPLE 71 30 g. of N -cinnamoyl-N -(p-methoxyphenyl)hydrazine hydrochloride was added into 50 g. of levulinic acid, and the mixture was heated at 75 C. for 2 hours under stirring. Thereafter the reaction mixture was poured into 200 ml. of water under vigorous stirring, the resultant crystals were collected by filtration, dried to give 34 g. of crude crystals of 1 cinnamoyl-2-methyl-5-methoxy-3-indolylacetic acid, M.P. 158-160 C., which was recrystallized from acetone twice. The melting point was raised to 164-165 C.
EXAMPLE 72 In a way similar to that in Example 71, l-(B-p-tolylacryloyl 2-methyl-5-methoxy-3-indolylacetic acid, M.P. 195 C., was prepared by reacting N (fl-p-tolyl-acryloyD- N (p methoxy-phenyDhydrazine hydrochloride with levulinic acid.
38 EXAMPLE 73 In a way similar to that in Example 71, l-(p-chlorocinnamoyl) 2-methyl-5-methoxy-3-indolylacetic acid, M.P. 220-221 C., was prepared by reacting N -(p-chlorocinnamoyl) N -(p-methoxyphenyl)hydrazine hydrochloride with levulinic acid.
Microanalysis.Calculated (percent): C, 65.71; H, 4.69; N, 3.65; Cl, 9.26. Found (percent): C, 65.46; H, 4.64; N, 3.56; Cl, 8.93.
EXAMPLE 74 According to a method similar to that in Example 71, 1 (u-methylcinnamoyl) 2-methyl-5-methoxy-3-indolylacetic acid, M.P. 153.5 154.5 C., was prepared by reacting N -(wmethylcinnamoyl)-N -(p-methoxyphenyl) hydrazine hydrochloride with levulinic acid.
EXAMPLE 75 20 g. of N -cinnamoyl-N -(p-methoxyphenyl)hydrazine hydrochloride and 12 g. of v-acetyl-n-butyric acid were added to 30 ml. of acetic acid, and the mixture was stirred at 75 C. for 2 hours. After completion of the reaction, the reaction mixture was allowed to cool, poured into 150 ml. of Water, and the resultant precipitates were collected by filtration, washed with water and dried to give 18 g. of crude crystals of p-(1-cinnamoyl-2-methyl-5-methoxy-3- indolyl)propionic acid, M.P. 182-186 C. The crude product was recrystallized from acetone-water to give 9.3 g. of the pure product, whereby the melting point was raised to 189190 C.
Microanalysis.-Calculated (percent): C, 72.71; H, 5.83; N, 3.58. Found (percent): C, 72.59; H, 5.96; N, 3.43.
EXAMPLE 76 In a way similar to that in Example 75, a-(Lcinnamoyl- 2 methyl 5-methoxy-3-indolyl)butyric acid, M.P. 126 C., was prepared by reacting N -cinnamoyl-N -(pmethoxyphenyl)hydrazine hydrochloride with e-acetyl-nvaleric acid.
Microanalysis.-Calculated (percent): C, 73.19; H, 6.14; N, 3.71. Found (percent): C, 73.23; H, 6.14; N, 3.61.
EXAMPLE 77 In a way similar to that in Example 75, yellow needle crystals of 1 (m-nitrocinnamoyl)-2-methyl-5-methoxy- 3-indolylacetic acid, M.P. 203 204 C., was prepared by reacting N -(m nitrocinnamoyl)-N -(p-methoxyphenyl) hydrazine hydrochloride with levulinic acid.
Microanalysis.-Calculated (percent): C, 63.94; H, 4.60; N, 7.10. Found (percent): C, 63.98; H, 4.66; N, 7.01.
EXAMPLE 78 5 g. of N -(fl-Z-furylacryloyl)-N -(p-methoxyphenyl)- hydrazine hydrochloride was added to 15 g. of levulinic acid, and the mixture was stirred at 75 80 C. for 2 hours. Thereafter, the mixture was allowed to cool and poured into cooled water. The resultant brown precipitates were collected by filtration, washed with water thoroughly. The washing liquor and the precipitates were combined and ether was added thereto, and then the mixture was shaken, and the ether layer Was separated. Similar procedures were repeated several times, and each ether layer was combined, dried on anhydrous sodium sulfate and concentrated to oily residue, which was recrystallized from methanol twice to give 3 g. of yellow crystals of 1-( ,8-2'- furylacryloyl) Z-methyl-S-methoxy-3-indolylacetic acid, M.P. 163.5-165 C.
Microanalysis.-Calculated (percent): C, 67.26; H, 5.01; N, 4.13. Found (percent): C, 67.26; H, 5.13; N, 3.94.
EXAMPLE 79 10 g. of N -cinnamoyl-N -(p-methoxyphenyl)hydrazine hydrochloride and 20 g. of a-methyl levulinic acid were added to 10 ml. of acetic acid and the mixture was stirred at 80 C. for 2 hours. The reaction mixture was allowed to stand to cool, and poured into water to give an oily substance, which was washed with water thoroughly, and extracted with each 100 ml. of ether three times. Each ether layer was combined and dried on anhydrous sodium sulfate, and ether was removed by distillation to give a residue, which was dissolved in ethyl acetate and columnchromatographed on neutralized active alumina to give crystals of a-(1-cinnamoyl-2-methyl-5-methoxy-3-indolyl) propionic acid. The recrystallization from acetone-water gave light yellow crystals of pure product, M.P. 154.5"- 155.5 C.
Micranalysis.Calculated (percent): C, 72.73; H, 5.79; N, 3.86. Found (percent): C, 72.97; H, 5.70; N, 3.92.
EXAMPLE 80 In a way similar to that in Example 75, l-(p-methoxycinnamoyl)-2-methyl-5-methoxy-3-indolylacetic acid, M.P. 193195 C., was prepared from N -(p-methoxycinnamoyl)-N (p-methoxyphenyl)hydrazine hydrochloride and levulinic acid.
Microanalysis.-Calculated (percent): C, 70.68; H, 5.72; N, 3.90. Found (percent): C, 69.97; H, 5.68; N, 3.79.
EXAMPLE 81 In a way similar to that in Example 71, ethyl l-cinnamoyl-2,5-dimethyl-3-indolylacetate, M.P. 198200 C., was prepared from N -cinnamoy1-N -(p-tolyl)hydrazine hydrochloride and ethyl levulinate.
EXAMPLE 82 In a Way similar to that in Example 71, l-ot-phenylcinnamoyl-Z-methyl-S-methOxy-3-indolylacetic acid, M.P. 174175 C., was prepared from N -ot-phenyl-cinnamoyl- N -(p-methoxyphenyl)hydrazine hydrochloride and levulinic acid.
EXAMPLE 83 According to a method similar to that in Example 71, 1-pheny1acetyl-2-methyl-5-methoxy 3 indolylacetic acid, M.P. 137l 39 C., was prepared from N -(phenylacetyl)- N -(p-methoxyphenyl)hydrazine hydrochloride and levulinic acid.
EXAMPLE 84 In a way similar to that in Example 71, l-(p-chlorocinnamoyl)-2-methyl-5-methoxy-3-indolylacetic acid, M.P. 220221 C., was prepared from N -(p-chlorocinnamoyl)- N -(p-methoxyphenyl)hydrazine hydrochloride and levulinic acid.
EXAMPLE 85 A mixture of 4.0 g. of N -(cc-chlorophenylacetyl)-N (p-rnethoxyphenyl)hydrazine hydrochloride and 2.4 g. of acetonylmalonic acid was heated in ml. of acetic acid at 85 C. for 4 hours with stirring. Thereafter, the reaction mixture was allowed to cool, and was poured into ml. of cold water, and then crystals were produced. They were collected by filtration, and dried to give crude product of 1-(a-chlorophenylacetyl)-2-rnethyl-5-methoxy 3 indolylacetic acid. The recrystallization from acetone-water gave fine white needle crystals, M.P. l65-166 C.
In a way similar to that in Example 85, the following compounds were prepared.
EXAMPLE 86 1-cinnamoyl-2-methyl-5-methoxy-3 indolylacetic acid,
M.P. 164-165 C.
EXAMPLE 87 l-phenylacetyl-2-methyl-5methoxy-3-indolylacetic acid, M.P. 14l.5143 C.
EXAMPLE 88 1-(ot-naphthylacetyl)-2-methyl-5-methoxy 3 indolylacetic acid, M.P. 165.5-166.5 C.
40 EXAMPLE 89 A mixture of 3.6 g. of N -(phenylacetyl)-N -(pmethoxyphenyl)hydrazine hydrochloride, and 2.4 g. of acetosuccinic acid was heated in 10 ml. of acetic acid at C. for 4 hours with stirring. Thereafter, the mixture was allowed to cool, and was poured into 25 ml. of cold water. The produced crystals were collected by filtration and dried to give a crude product of l-(phenylacety-D- 2-methyl-5-methoxy-3-indolylacetic acid. The recrystallization from acetone-water gave white needle crystals, M.P. 142-143 C.
By this method, the same product was obtained by using Z-keto-adipic acid instead of acetosuccinic acid.
EXAMPLE 90 In a way similar to that in Example 89, 1-cinnamoy1-2- methyl-5-methoxy-3-indolylacetic acid, M.P. 164165 C., was prepared from N -cinnamoyl-N -(p-methoxyphenyl)hydrazine hydrochloride and acetosuccinic acid.
EXAMPLE 91 4.8 g. of tertiary butyl l-(phenylacetyl)-2-methyl-5- methoxy-3-indolylacetate was added to 40 m1. of benzene, then a small amount of p-toluenesulfonic acid was added thereto. The reaction mixture was refluxed to complete the reaction. After the heating, the mixture was left at room temperature. After cooling it was washed with 60 ml. of water twice, and was dried. The reaction s lvent was removed by distillation, and the resultant residue was purified and crystallized by using acetone and recrystallized from acetone-water to give l-(phenylacetyl)-2-methyl-5- methoxy-3-indolylacetic acid, M.P. 138139 C.
In a Way similar to that in Example 91, the following compounds were prepared.
EXAMPLE 92 1-( ot-naphthylacetyl) -2-methyl-5 methoxy 3 indolylacetic acid, M.P. 165167 C.
EXAMPLE 93 l-{fl-(p-tolyl)acryloyl}-2-methyl-5-methoxy-3 ind lylacetic acid, M.P. 195 196 C.
EXAMPLE 94 l-(p-chlorocinnamoyl)-2-methy1-5-methoxy-3 indolylacetic acid, M.P. 220221 C.
EXAMPLE 95 'y-(1-cinnamoyl-2-methyl-5-methoxy-3 indolyl)butyric acid, M.P. 125-l26 C.
EXAMPLE 96 1-( fl-T-furylacryloyl)-2-methyl 5 methoxy3-indolylacetic acid, M.P. 163165 C.
EXAMPLE 97 et-(l-cinnamoyl2-methyl 5 methoxy-3-indolyl)propionic acid, M.P. l54-156 C.
EXAMPLE 98 1-(p-methoxycinnamoyl)-2-methy1 5 methoxy-3-indolylacetic acid, M.P. 193 195 C.
EXAMPLE 99 l-cinnamoyl-Z-methyl-S -methoxy 3 indolylacetic acid, M.P. 164 C.
EXAMPLE 100 7.6 g. of acetaldehyde N -phenylacetyl-N -(p-methoxyphenyl)hydrazone was added to 30* g. of levulinic acid containing 1 g. of hydrogen chloride, and the mixture was heated at 80 C. for 3 hours. Thereafter, the mixture was allowed to cool, and poured into 200* ml. of water. And then the solid produced was collected by filtration, and was column-chromatographed on silica gel by using ethyl acetate as developer and then was re-
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US4029798A (en) * 1975-06-02 1977-06-14 Sumitomo Chemical Company, Limited Analgetic composition
US5721347A (en) * 1990-11-06 1998-02-24 Cell Pathways, Inc. Esters and amides of substituted pyrrole acetic acids

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4029798A (en) * 1975-06-02 1977-06-14 Sumitomo Chemical Company, Limited Analgetic composition
US5721347A (en) * 1990-11-06 1998-02-24 Cell Pathways, Inc. Esters and amides of substituted pyrrole acetic acids

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