US20070219233A1

US20070219233A1 - Substituted Indoles and Their Use as Integrin Antagonists

Info

Publication number: US20070219233A1
Application number: US11/755,831
Authority: US
Inventors: Tianbo Lu; Bruce Tomczuk; Louis LaFrance; Thomas Markotan; Juan Marugan Sanchez; Victor Marder; David U'Prichard; Beth Anaclerio; Zihong Guo; Wenzi Pan; Kristi Leonard
Original assignee: Individual
Current assignee: Individual
Priority date: 2001-01-29
Filing date: 2007-05-31
Publication date: 2007-09-20
Also published as: US7241789B2; KR100854424B1; CA2436130A1; ATE530183T1; WO2002060438A1; US20050209225A1; KR20030088024A; US20020169200A1; AU2002243692B2; JP4615826B2; US6855722B2; EP1425010B1; EP1425010B8; MXPA03006772A; US20050250771A1; EP1425010A4; ES2376161T3; EP1425010A1; JP2004533993A; NZ539682A

Abstract

The present invention relates to novel substituted indole compounds that are antagonists of alpha V (αv) integrins, for example α_vβ₃and α_vβ₅integrins, their pharmaceutically acceptable salts, and pharmaceutical compositions thereof. The compounds may be used in the treatment of pathological conditions mediated by α_vβ₃and α_vβ₅integrins, including such conditions as tumor growth, metastasis, restenosis, osteoporosis, inflammation, macular degeneration, diabetic retinopathy, and rheumatoid arthritis. The compounds have the general formula:

where R¹, R², R³, R⁴, R⁵, R⁶, R⁷, R⁸, R⁹, R¹⁰, R¹¹, R¹², R¹³, R¹⁴, D, X, W, a, m, n, i, j, k and v are defined herein.

Description

BACKGROUND OF THE INVENTION

1. Field of the Invention
The present invention relates to novel substituted indole compounds that are antagonists of alpha V (αv) integrins, for example α_vβ₃and α_vβ₅integrins, their pharmaceutically acceptable salts, and pharmaceutical compositions thereof.
2. Related Art
Integrins are cell surface glycoprotein receptors which bind extracellular matrix proteins and mediate cell-cell and cell-extracellular matrix interactions (generally referred to as cell adhesion events) (Hynes, R. O., Cell 69:11-25 (1992)). These receptors are composed of noncovalently associated alpha (α) and beta (β) chains which combine to give a variety of heterodimeric proteins with distinct cellular and adhesive specificities (Albeda, S. M., Lab. Invest. 68:4-14 (1993)). Recent studies have implicated integrins in the regulation of cellular adhesion, migration, invasion, proliferation, apoptosis and gene expression (Albeda, S. M., Lab. Invest. 68:4-14 (1993); Juliano, R., Cancer Met. Rev. 13:25-30 (1994); Ruoslahti, E. and Reed, J. C., Cell 77:477-478 (1994); and Ruoslahti, E. and Giancotti, F. G., Cancer Cells 1:119-126 (1989)).
One member of the integrin family which has been shown to play a significant role in a number of pathological conditions is the integrin α_vβ₃, or vitronectin receptor (Brooks, P. C., DN&P 10(8):456-461 (1997)). This integrin binds a variety of extracellular matrix components and other ligands, including fibrin, fibrinogen, fibronectin, vitronectin, laminin, thrombospondin, and proteolyzed or denatured collagen (Cheresh, D. A., Cancer Met. Rev. 10:3-10 (1991) and Shattil, S. J., Thromb. Haemost. 74:149-155 (1995)). The two related αv integrins, α_vβ₅and α_vβ₁(also vitronectin receptors), are more specific and bind vitronectin (α_vβ₅) or fibronectin and vitronectin (α_vβ₁) (Horton, M., Int. J. Exp. Pathol. 71:741-759 (1990)). α_vβ₃and the other integrins recognize and bind to their ligands through the tripeptide sequence Arg-Gly-Asp (“RGD”) (Cheresh, D. A., Cancer Met. Rev. 10:3-10 (1991) and Shattil, S. J., Thromb. Haemost. 74:149-155 (1995)) found within all the ligands mentioned above.
The α_vβ₃integrin has been implicated in a number of pathological processes and conditions, including metastasis and tumor growth, pathological angiogenesis, and restenosis. For example, several studies have clearly implicated α_vβ₃in the metastatic cascade (Cheresh, D. A., Cancer Met. Rev. 10:3-10 (1991); Nip, J. et al., J. Clin. Invest. 95:2096-2103 (1995); and Yun, Z., et al., Cancer Res. 56:3101-3111 (1996)). Vertically invasive lesions in melanomas are also commonly associated with high levels of α_vβ₃, whereas horizontally growing noninvasive lesions have little if any α_vβ₃(Albeda, S. M., et al., Cancer Res. 50:6757-6764 (1990)). Moreover, Brooks et al. (in Cell 79:1157-1164 (1994)) have demonstrated that systemic administration of α_vβ₃antagonists disrupts ongoing angiogenesis on chick chorioallantoic membrane (“CAM”), leading to the rapid regression of histologically distinct human tumors transplanted onto the CAM. These results indicate that antagonists of α_vβ₃may provide a therapeutic approach for the treatment of neoplasia (solid tumor growth).
α_vβ₃has also been implicated in angiogenesis, which is the development of new vessels from preexisting vessels, a process that plays a significant role in a variety of normal and pathological biological events. It has been demonstrated that α_vβ₃is up-regulated in actively proliferating blood vessels undergoing angiogenesis during wound healing as well as in solid tumor growth. Also, antagonists of α_vβ₃have been shown to significantly inhibit angiogenesis induced by cytokines and solid tumor fragments (Brooks, P. C., et al., Science 264:569-571 (1994); Enenstein, J. and Kramer, R. H., J. Invest. Dermatol. 103:381-386 (1994); Gladson, C. L., J. Neuropathol. Exp. Neurol 55:1143-1149 (1996); Okada, Y., et al., Amer. J. Pathol. 149:37-44 (1996); and Brooks, P. C., et al., J. Clin. Invest. 96:1815-1822 (1995)). Such α_vβ₃ antagonists would be useful for treating conditions that are associated with pathological angiogenesis, such as rheumatoid arthritis, diabetic retinopathy, macular degeneration, and psoriasis (Nicosia, R. F. and Madri, J. A., Amer. J. Pathol. 128:78-90 (1987); Boudreau, N. and Rabinovitch, M., Lab. Invest. 64:187-99 (1991); and Brooks, P. C., Cancer Met. Rev. 15:187-194 (1996)).
There is also evidence that α_vβ₃plays a role in neointimal hyperplasia after angioplasty and restenosis. For example, peptide antagonists and monoclonal antibodies directed to both α_vβ₃and the platelet receptor α_vβ₃have been shown to inhibit neointimal hyperplasia in vivo (Choi, E. T., et al., J. Vasc. Surg. 19:125-134 (1994); and Topol, E. J., et al., Lancet 343:881-886 (1994)), and recent clinical trials with a monoclonal antibody directed to both αII_bβ₃and α_vβ₃have resulted in significant reduction in restenosis, providing clinical evidence of the therapeutic utility of β3 antagonists (Topol, E. J., et al., Lancet 343:881-886 (1994)).
It has also been reported that α_vβ₃is the major integrin on osteoclasts responsible for attachment to bone. Osteoclasts cause bone resorption. When bone resorbing activity exceeds bone forming activity, the result is osteoporosis, a condition which leads to an increased number of bone fractures, incapacitation and increased mortality. Antagonists of α_vβ₃have been shown to be potent antagonists of osteoclastic activity both in vitro (Sato, M., et al., J. Cell Biol. 111:1713-1723 (1990)) and in vivo (Fisher, J. E., et al., Endocrinology 132:1411-1413 (1993)).
Lastly, White (in Current Biology 3(9):596-599 (1993)) has reported that adenovirus uses α_vβ₃for entering host cells. The α_vβ₃integrin appears to be required for endocytosis of the virus particle and may be required for penetration of the viral genome into the host cell cytoplasm. Thus compounds which inhibit α_vβ₃could be useful as antiviral agents.
The α_vβ₅integrin has been implicated in pathological processes as well. Friedlander et al. have demonstrated that a monoclonal antibody for α_vβ₅can inhibit VEGF-induced angiogenesis in rabbit cornea and chick chorioallantoic membrane, indicating that the α_vβ₅integrin plays a role in mediating growth factor-induced angiogenesis (Friedlander, M. C., et al., Science 270:1500-1502 (1995)). Compounds that act as α_vβ₅antagonists could be used to inhibit pathological angiogenesis in tissues of the body, including ocular tissue undergoing neovascularization, inflamed tissue, solid tumors, metastases, or tissues undergoing restenosis.
Discovery of the involvement of α_vβ₃and α_vβ₅in such processes and pathological conditions has led to an interest in these integrins as potential therapeutic targets, as suggested in the preceding paragraphs. A number of specific antagonists of α_vβ₃and α_vβ₅that can block the activity of these integrins have been developed. One major group of such antagonists includes nonpeptide mimetics and organic-type compounds. For example, a number of organic non-peptidic mimetics have been developed that appear to inhibit tumor cell adhesion to a number of α_vβ₃ligands, including vitronectin, fibronectin, and fibrinogen (Greenspoon, N., et al., Biochemistry 32:1001-1008 (1993); Ku, T. W., et al., J. Amer. Chem. Soc. 115:8861-8862 (1993); Hershkoviz, R., et al., Clin. Exp. Immunol. 95:270-276 (1994); and Hardan, L., et al., Int. J. Cancer 55:1023-1028 (1993)).
Additional organic compounds developed specifically as α_vβ₃or α_vβ₅integrin antagonists or as compounds useful in the treatment of αv-mediated conditions have been described in several recent publications.
For example, U.S. Pat. No. 5,741,796, issued Apr. 21, 1998, discloses pyridyl and naphthyridyl compounds for inhibiting osteoclast-mediated bone resorption.
PCT Published Application WO 97/45137, published Oct. 9, 1997, discloses non-peptide sulfotyrosine derivatives, as well as cyclopeptides, fusion proteins, and monoclonal antibodies, that are useful as antagonists of α_vβ₃integrin-mediated angiogenesis.
PCT Published Application WO 97/36859, published Oct. 9, 1997, discloses para-substituted phenylpropanoic acid derivatives of the general formula:
where A is:
B is —CH₂CONH—, —CONR⁵²—(CH₂)_p—, —C(O)O—, —SO₂NH—, —CH₂O—, or —OCH₂—;
Y¹is selected from the group consisting of N—R², O and S;
Y³and Z³are independently selected from the group consisting of H, alkyl, aryl, cycloalkyl and aralkyl, or Y³and Z³taken together with C form a carbonyl;
R⁵⁰is selected from the group consisting of H, alkyl, aryl, carboxyl derivative and —CONHCH₂CO₂R⁵³, wherein R⁵³is H or lower alkyl; and
R⁵¹is selected from the group consisting of H, alkyl, carboxyl derivatives,
wherein R⁵⁴is selected from the group consisting of H, alkyl, cycloalkyl, aryl, aralkyl, aralkenyl and aryl substituted by one or more alkyl or halo; and wherein R⁵⁵is selected from the group consisting of N-substituted pyrrolidinyl, piperidinyl and morpholinyl.
The publication also discloses the use of the compounds as α_vβ₃integrin antagonists.
PCT Published Application WO 97/06791, published February 1997, discloses methods for inhibition of angiogenesis in tissue using vitronectin α_vβ₅antagonists.
More recently, PCT Published Application WO 97/23451, published Jul. 3, 1997, discloses tyrosine derivatives of the general formula:

wherein
X is C_1-6alkylene or 1,4-piperidyl;
Y is absent, O, CONH or —C^˜C—;
R¹is H, CN, N₃, NH₂, H₂N—C(═NH), or H₂N—C(═NH)—NH, where the primary amino groups can also be provided with conventional amino protective groups;
R²and R³are independently H, A, A-SO₂—, Ar—SO₂—, camphor-10-SO₂—, COOA or a conventional amino protective group;
A and R⁴are independently H, C_1-10alkyl, or benzyl; and
Ar is phenyl or benzyl, each of which is unsubstituted or monosubstituted by CH₃;
and their physiologically acceptable salts.
The disclosed compounds are described as αv-integrin antagonists (especially α_vβ₃ antagonists) useful in the treatment of tumors, osteoporoses, and osteolytic disorders and for suppressing angiogenesis.
PCT Published Application WO 98/00395, published Jan. 8, 1998, discloses novel tyrosine and phenylalanine derivatives as αv integrin and GPIIb/IIIa antagonists having the general formula:

wherein
X can be, among other groups, alkyl, aryl or cycloalkyl;
Y and Z can be alkyl, O, S NH, C(═O), CONH, NHCO, C(═S), SO₂NH, NHSO₂, CA=CA! or —C^˜C—;
R¹can be H₂N—C(═NH) or H₂N—(C═NH)—NH;
R²is A, aryl or aralkyl;
R³is hydrogen or A;
R⁴is hydrogen, halogen, OA, NHA, NAA!, —NH-Acyl, —O-Acyl, CN, NO₂, SA, SOA, SO₂A, SO₂Ar or SO₃H; and
A and A! can be hydrogen, alkyl or cycloalkyl.
The publication discloses the use of the compounds in pharmaceutical preparations for the treatment of thrombosis, infarction, coronary heart disease, tumors, arteriosclerosis, infection and inflammation.

SUMMARY OF THE INVENTION

The present invention is directed to substituted indoles having Formula IV (below). Also provided is a process for preparing compounds of Formula IV. The novel compounds of the present invention exhibit inhibition of α_vβ₃and α_vβ₅integrin receptor binding. Also provided is a method of treating α_vβ₃integrin- and α_vβ₅integrin-mediated pathological conditions such as tumor growth, metastasis, osteoporosis, restenosis, inflammation, macular degeneration, diabetic retinopathy, sickle cell anemia, CNS disorders and rheumatoid arthritis in a mammal in need of such treatment comprising administering to said mammal an effective amount of a compound of Formula IV. Further provided is a pharmaceutical composition comprising a compound of Formula IV and one or more pharmaceutically acceptable carriers or diluents.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

The present invention is directed to compounds of Formula IV:

and pharmaceutically acceptable salts thereof, wherein
R¹, R², R³, R⁴and R⁵independently represent hydrogen, halogen, alkyl, aryl, aralkyl, heteroaryl or heteroarylalkyl;
R⁶, R⁷, R⁵and R⁹independently represent hydrogen, alkyl, hydroxyalkyl, aminoalkyl, monoalkylaminoalkyl, dialkylaminoalkyl, carboxyalkyl, aryl or aralkyl;
or R⁶and R⁷are taken together to form —(CH₂)_p—, where p is 2-8, while R⁵and R⁹are defined as above; or R⁵and R⁹are taken together to form —(CH₂)_q—, where q is 2-8, while R⁶and R⁸are defined as above; or R⁶and R⁸are taken together to form —(CH₂)_r—, while r is zero (a bond), 1 or 2, while R⁷and R⁹are defined as above;
X represents oxygen, sulfur, —CH₂—, —NH—, —(C═O)NH— or —NH(C═O)—;
n is from 0 to 4;
m is from 0 to 4;
a is 0 or 1;
D represents oxygen;
v is 0 or 1;
R¹⁰, R¹¹, R¹²and R¹³independently represent: hydrogen; hydroxy; alkyl; alkoxy; cycloalkyl; aryl, optionally substituted with one or more of halogen, hydroxy, cyano, alkyl, aryl, alkoxy, haloalkyl, arylalkyl, arylalkoxy, aryloxy, alkylsulfonyl, alkylsulfinyl, alkoxyarylalkyl, monoalkylamino, dialkylamino, aminoalkyl, monoalkylaminoalkyl, dialkylaminoalkyl, alkanoyl; monoalkylamino; dialkylamino; aminoalkyl; monoalkylaminoalkyl; dialkylaminoalkyl; alkanoyl; heteroaryl having 5-14 ring members, optionally substituted with one or more of halogen, hydroxy, cyano, alkyl, aryl, alkoxy, haloalkyl, arylalkyl, arylalkoxy, aryloxy, alkylsulfonyl, alkylsulfinyl, alkoxyarylalkyl, monoalkylamino, dialkylamino, aminoalkyl, monoalkylaminoalkyl, dialkylaminoalkyl, alkanoyl; or
wherein R¹⁷and R¹⁸together form —CH₂CH₂—O—, —O—CH₂CH₂—, —O—CH₂—O— or —O—CH₂CH₂—O—; or
R¹⁰and R¹²are taken together to form —(CH₂)_s—, wherein s is 0 (a bond) or 1 to 4, while R¹¹and R¹³are as defined as above; or R¹⁰and R¹²are taken together to form a double bond when i is 0 and k is 1, while R¹¹and R¹³are as defined above; or R¹⁰and R¹¹are taken together to form —(CH₂)_t—, wherein t is 2 to 8, while R¹²and R¹³are as defined as above, or R¹²and R¹³are taken together to form —(CH₂)_u— wherein u is 2 to 8, while R¹⁰and R¹¹are as defined as above;
i is from 0 to 4;
j is from 0 to 4;
k is 0 or 1;
R¹⁴is hydrogen or a functionality that acts as a prodrug (i.e., converts to the active species by an endogenous biological process such as an esterase, lipase, or other hydrolase), such as alkyl, aryl, aralkyl, dialkylaminoalkyl, 1-morpholinoalkyl, 1-piperidinylalkyl, pyridinylalkyl, alkoxy(alkoxy)alkoxyalkyl, or (alkoxycarbonyl)oxyethyl;
W is:

wherein:
Y is —N— or —CH—;
Z is —N— or —CH—;
R¹⁵is hydrogen, halogen, alkyl, aryl or arylalkyl;
R¹⁶is hydrogen, alkyl, haloalkyl or halogen;
R¹⁹and R²⁰are independently hydrogen, halogen or alkyl;
R²⁷, R²⁸, R²⁹, R³⁰and R³¹are independently hydrogen, halogen, alkyl, alkoxy or aryl; and
o and p are independently 0, 1 or 2.
Where W is attached through a pyridine ring, the preferred point of attachment is either ortho or meta to the pyridine nitrogen, and more preferably ortho to the pyridine nitrogen.
Preferred compounds of the present invention are those of Formula IV, wherein R¹and R²independently represent hydrogen, halogen, (C_1-6)alkyl, (C_1-6)aryl, (C_1-6)alkyl(C_6-10)aryl, (C_6-10)ar(C_1-6)alkyl, 5-14 member heteroaryl, or 5-14 member heteroaryl(C_1-6)alkyl; or preferably R¹and R²independently represent hydrogen, methyl, ethyl, propyl, butyl, phenyl, benzyl or phenylethyl.
Also preferred are compounds of Formula IV, wherein R³, R⁴and R⁵independently represent hydrogen, (C_1-6)alkyl, (C_6-10)aryl, or (C_6-10)ar(C_1-6) alkyl, preferably, R³, R⁴and R⁵are hydrogen or (C_1-4)alkyl.
Preferred compounds are those of Formula IV, wherein R⁶, R⁷, R⁵and R⁹independently represent hydrogen or (C_1-4)alkyl.
Preferred compounds are those of Formula IV, wherein X is oxygen, —CH₂—, —(C═O)NH— or —HN(C═O)—, more preferably, X is oxygen, —CH₂— or —(C═O)NH—.
Also preferred are compounds of Formula IV, wherein W is

more preferably

wherein Y R¹⁵, R¹⁶, R¹⁹, R²⁰, R²⁷-R³¹are as defined above;
More preferably,
Y is —N— or —CH—;
R¹⁵is hydrogen, halogen, (C_1-8)alkyl, (C_6-10)aryl or (C_6-10)aryl(C_1-8)alkyl;
R¹⁶is hydrogen, (C_1-8)alkyl, halo(C_1-8)alkyl or halogen;
R¹⁹and R²⁰are hydrogen, halogen or (C_1-8)alkyl; and
R²⁷, R²⁸, R²⁹, R³⁰and R³¹are hydrogen, halogen, (C_1-8)alkyl, (C_1-8)alkoxy, (C_6-10)aryl.
Further preferred compounds are those of Formula IV, wherein R¹⁰, R¹¹, R¹²and R¹³independently represent hydrogen, hydroxy, (C_1-6)alkyl, (C_3-6)cycloalkyl, (C_6-10)aryl, (C_6-10)ar(C_1-6)alkyl, (C_1-6)aminoalkyl, mono(C_1-4) alkylamino(C_1-6)alkyl, di-(C_1-4)alkylamino (C_1-6)alkyl, carboxy(C_1-6)alkyl, (C_1-6)alkoxy, mono-(C_1-4)alkylamino or di-(C_1-4)alkylamino.
Also preferred are those compounds of Formula IV, wherein R¹⁰and R¹²are taken together to form —(CH₂)_s— where s is zero or 1 to 4, and R¹¹and R¹³are each hydrogen.
Preferred compounds are those of Formula IV, wherein R¹⁰and R¹¹are taken together to form —(CH₂)_t, where t is 2 to 5 and R¹²and R¹³are each hydrogen.
Preferred compounds are also those wherein R¹²and R¹³are independently,

wherein:
b is from 0 to 4;
R³²is halogen, (C_1-8)alkyl, halo(C_1-8)alkyl, (C_1-8)alkoxy, (C_1-8)alkoxy(C_1-8)alkyl or halo(C_1-8)alkoxy;
R³³is halogen;
R³⁴is (C_1-8)alkyl, hydroxy or (C_1-8)alkoxy; or
two of R³², or two of R³³, or one of R³³and R³⁴, when attached to adjacent carbon atoms, may together form a ring, wherein the ring formed is an aliphatic, aryl or heteroaryl ring, each of which may be optionally substituted by one or more of halogen, hydroxy, cyano, alkyl, aryl, alkoxy, haloalkyl, arylalkyl, arylalkoxy, aryloxy, alkylsulfonyl, alkylsulfinyl, alkoxyarylalkyl, monoalkylamino, dialkylamino, aminoalkyl, monoalkylaminoalkyl, dialkylaminoalkyl, alkanoyl; monoalkylamino; dialkylamino; aminoalkyl; monoalkylaminoalkyl; dialkylaminoalkyl; alkanoyl.
Preferred compounds of the present invention include, but are not limited to, those compounds wherein R¹²and R¹³are independently selected from:
Additional preferred compounds of Formula IV, are those wherein R¹⁰and R¹²are taken together to form a double bond where i is 0 and k is 1, and R¹¹and R¹³are each hydrogen.
Preferred compounds of the invention are also those wherein R¹⁰is an optionally substituted aryl or optionally substituted heteroaryl.
Additionally, preferred compounds of the invention may contain an alkenyl carboxylic acid moiety.
Further preferred compounds are those of Formula IV, wherein i and j are 0.
Preferred compounds are those of Formula IV, wherein k is 1.
Also preferred compounds are those of Formula IV, wherein R¹⁴is hydrogen.
Preferred compounds are those of Formula IV, wherein i and j are each zero; k is one; R¹⁰, R¹¹and R¹²are each hydrogen; and R¹³is hydrogen, C_1-6alkyl, C_6-10aryl or C_6-10ar(C_1-4) alkyl.
Preferred compounds of the present invention are those of Formula IV wherein:
R¹is hydrogen or (C_1-4)alkyl, more preferably, hydrogen or methyl;
R², R³, R⁴, and R⁵are hydrogen or (C_1-4)alkyl, more preferably hydrogen;
R⁶, R⁷, R⁵and R⁹are preferably hydrogen or (C_1-4)alkyl, more preferably hydrogen;
X is oxygen or —CH₂—;
n is 0 or 1;
m is 0 or 1;
R¹⁰, R¹¹, R¹²and R¹³independently represent hydrogen, (C_1-6)alkyl or (C_6-10)ar(C_1-6)alkyl; or
one of the combination R¹⁰or R¹¹, R¹²or R¹³, R¹⁰and R¹²are taken together to form —(CH₂)_s—, wherein s is 1 or 2 while the remaining R¹⁰—R¹³are defined above;
i is 0 or 1;
j is 0 or 1;
k is 0 or 1;
R¹⁴is hydrogen, C_1-6alkyl or benzyl;
W is:

wherein:
Y is —N— or —CH—;
R¹⁵is hydrogen, halogen, (C_1-8)alkyl, (C_6-10)aryl or (C_6-10)aryl(C_1-8)alkyl;
R¹⁶is hydrogen, (C_1-8)alkyl, halo(C_1-8)alkyl or halogen;
R¹⁹and R²⁰are hydrogen, halogen or (C_1-8)alkyl; and
R²⁷, R²⁸, R²⁹, R³⁰and R³¹are hydrogen, halogen, (C_1-8)alkyl, (C_1-8)alkoxy, (C_6-10)aryl.
Additionally preferred compounds of Formula IV are those wherein:

- X is —(C═O)NH—;
- n, m, a and v are each 0; and
- R⁶, R⁷, R¹²and R¹³are hydrogen.

Further preferred compounds of Formula IV are those wherein:

- X is oxygen;
- n and m are each 0;
- a and v are each 1;
- D is oxygen;
- R⁶, R⁷, R⁸and R⁹are hydrogen.

Preferred compounds of Formula IV are also those wherein:

- X is oxygen;
- n, m and v are each 0;
- a is 1; and
- R⁶, R⁷, R¹²and R¹³are hydrogen.

Further preferred compounds of Formula IV are also those wherein:

- X is —CH₂—;
- n, m and v are each 0;
- a is 1; and
- R⁶, R⁷, R¹²and R¹³are hydrogen.

Examples of useful compounds of the present invention include:

3-{5-[3-(2-pyridylamino)propoxy]indolyl}propanoic acid;
3-{5-[3-(2-pyridylamino)propoxy]indolyl}acetic acid;
3-{2-methyl-5-[3-(2-pyridylamino)propoxy]indolyl}propanoic acid;
2-(trans-2-{5-[3-(2-pyridylamino)propoxy]indolyl}cyclopropyl)acetic acid;
3-(5-{2-[6-(methylamino)-2-pyridyl]ethoxy}indolyl)propanoic acid;
2-benzyl-3-{5 [3-(2-pyridylamino)propoxy]indolyl}propanoic acid;
2-methyl-3-{5-[3-(2-pyridylamino)propoxy]indolyl}propanoic acid;
2-({5-[3-(2-pyridylamino)propoxy]indolyl}methyl)pentanoic acid;
2-({5-[3-(2-pyridylamino)propoxy]indolyl}methyl)octanoic acid;
3-[5-(3-{[benzylamino]carbonylamino}propoxy)indolyl]propanoic acid;
3-[5-(2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl-acetylamino)-indol-1-yl]-hexanoic acid;
3-(5-{2-[N-(4,5-dihydro-1H-imidazol-2-yl)-aminooxy]-ethoxy}-indol-1-yl)-3-phenyl-propionic acid;
3-(5-{2-[guanidino-oxy]-ethoxy}-indol-1-yl)-3-phenyl-propionic acid;
3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-hexanoic acid;
3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-phenyl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-phenyl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-(3-benzyloxy-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-p-tolyl-propionic acid;
3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-m-tolyl-propionic acid;
3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-O— tolyl-propionic acid;
3-biphenyl-4-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-(3,5-dichloro-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-(3,5-difluoro-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-(3-cyano-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-(4-cyano-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-(2-methoxy-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-(3-methoxy-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-(4-methoxy-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-quinolin-3-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-(3-chloro-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-naphthalen-2-yl-3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-(2-chloro-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-naphthalen-1-yl-3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-(4-fluoro-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-(3-trifluoromethyl-phenyl)-propionic acid;
3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-(4-trifluoromethyl-phenyl)-propionic acid;
3-pyridin-3-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-pyridin-2-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-pyridin-4-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-acrylic acid;
3-(2,3-dihydro-benzofuran-5-yl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-benzo[1,3]dioxol-5-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-(5-methanesulfonyl-pyridin-3-yl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethyoxy]-indol-1-yl}-propionic acid;
3-{5-[2-(6-methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-3-phenyl-propionic acid;
3-{5-[2-(6-methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-3-quinolin-3-yl-propionic acid;
3-{5-[2-(6-methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-3-pyridin-3-yl-propionic acid;
3-{5-[2-(6-methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-hexanoic acid;
3-{5-[2-(2-methyl-5,6,7,8-tetrahydro-[1,8]naphthyridin-3-yl)-ethyl]-indol-1-yl}-propionic acid;
3-{5-[3-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-propyl]-indol-1-yl}-propionic acid;
3-{5-[3-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-propyl]-indol-1-yl}-hexanoic acid;
3-phenyl-3-{5-[3-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-propyl]-indol-1-yl}-propionic acid;
3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-[5-(2,2,2-trifluoro-ethoxy)-pyridin-3-yl]-propionic acid;
3-(5-Ethoxy-pyridin-3-yl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-Pyridin-4-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-Pyridin-2-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-acrylic acid;
6-(2-hydroxy-ethyl)-2,3-dihydro-pyrido[3,2-b][1,4]oxazine-4-carboxylic acid tert-butyl ester;
3-{5-[2-(6-methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-3-quinolin-3-yl-propionic acid;

or a pharmaceutically acceptable salt, hydrate, solvate or prodrug thereof.
Particularly preferred compounds of the invention are:

3-(3-methoxy-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-quinolin-3-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-pyridin-3-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-pyridin-2-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid;
3-{5-[2-(6-methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-3-quinolin-3-yl-propionic acid;

or a pharmaceutically acceptable salt, hydrate, solvate or prodrug thereof.
It is also to be understood that the present invention is considered to include stereoisomers as well as optical isomers, e.g. mixtures of enantiomers as well as individual enantiomers and diastereomers, which arise as a consequence of structural asymmetry in selected compounds of the present series.
When any variable occurs more than one time in any constituent or in Formula IV, its definition on each occurrence is independent of its definition at every other occurrence. Also, combinations of substituents and/or variables are permissible only if such combinations result in stable compounds.
The term “alkyl” as employed herein by itself or as part of another group refers to both straight and branched chain radicals of up to 12 carbons, preferably 1 to 8 carbons, such as methyl, ethyl, propyl, isopropyl, butyl, t-butyl, isobutyl, pentyl, hexyl, isohexyl, heptyl, 4,4-dimethylpentyl, octyl, 2,2,4-trimethylpentyl, nonyl, decyl, undecyl, dodecyl.
The term “alkenyl” is used herein to mean a straight or branched chain radical of 2-20 carbon atoms, unless the chain length is limited thereto, including, but not limited to, ethenyl, 1-propenyl, 2-propenyl, 2-methyl-1-propenyl, 1-butenyl, 2-butenyl, and the like. Preferably, the alkenyl chain is 2 to 10 carbon atoms in length, more preferably, 2 to 8 carbon atoms in length most preferably from 2 to 4 carbon atoms in length.
The term “alkoxy” is used herein to mean a straight or branched chain radical of 1 to 20 carbon atoms, unless the chain length is limited thereto, bonded to an oxygen atom, including, but not limited to, methoxy, ethoxy, n-propoxy, isopropoxy, and the like. Preferably the alkoxy chain is 1 to 10 carbon atoms in length, more preferably 1 to 8 carbon atoms in length.
The term “aryl” as employed herein by itself or as part of another group refers to monocyclic or bicyclic aromatic groups containing from 6 to 12 carbons in the ring portion, preferably 6-10 carbons in the ring portion, such as phenyl, naphthyl or tetrahydronaphthyl.
The term Aaryloxy≅ as employed herein by itself or as part of another group refers to monocyclic or bicyclic aromatic groups containing from 6 to 12 carbons in the ring portion, preferably 6-10 carbons in the ring portion, bonded to an oxygen atom. Examples include, but are not limited to, phenoxy, naphthoxy, and the like.
The term Aheteroaryl≅ as employed herein refers to groups having 5 to 14 ring atoms; 6, 10 or 14 π electrons shared in a cyclic array; and containing carbon atoms and 1, 2 or 3 oxygen, nitrogen or sulfur heteroatoms (where examples of heteroaryl groups are: thienyl, benzo[b]thienyl, naphtho[2,3-b]thienyl, thianthrenyl, furyl, pyranyl, isobenzofuranyl, benzoxazolyl, chromenyl, xanthenyl, phenoxathiinyl, 2H-pyrrolyl, pyrrolyl, imidazolyl, pyrazolyl, pyridyl, pyrazinyl, pyrimidinyl, pyridazinyl, indolizinyl, isoindolyl, 3H-indolyl, indolyl, indazolyl, purinyl, 4H-quinolizinyl, isoquinolyl, quinolyl, phthalazinyl, naphthyridinyl, quinazolinyl, cinnolinyl, pteridinyl, 4H-carbazolyl, carbazolyl, β-carbolinyl, phenanthridinyl, acridinyl, perimidinyl, phenanthrolinyl, phenazinyl, isothiazolyl, phenothiazinyl, isoxazolyl, furazanyl and phenoxazinyl groups).
The term “aralkyl” or “arylalkyl” as employed herein by itself or as part of another group refers to C_1-6alkyl groups as discussed above having an aryl substituent, such as benzyl, phenylethyl or 2-naphthylmethyl.
The term “cycloalkyl” as employed herein by itself or as part of another group refers to cycloalkyl groups containing 3 to 9 carbon atoms. Typical examples are cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl and cyclononyl.
The term “heterocycle” or “heterocyclyl” as used herein, except where noted, represents a stable 5- to 7-membered mono- or bicyclic or stable 7- to 10-membered bicyclic heterocyclic ring system any ring of which may be saturated or unsaturated, and which consists of carbon atoms and from one to three heteroatoms selected from the group consisting of N, O and S, and wherein the nitrogen and sulfur heteroatoms may optionally be oxidized, and the nitrogen heteroatom may optionally be quaternized, and including any bicyclic group in which any of the above-defined heterocyclic rings is fused to a benzene ring. Especially useful are rings containing one oxygen or sulfur, one to three nitrogen atoms, or one oxygen or sulfur combined with one or two nitrogen atoms. The heterocyclic ring may be attached at any heteroatom or carbon atom which results in the creation of a stable structure. Examples of such heterocyclic groups include piperidinyl, piperazinyl, 2-oxopiperazinyl, 2-oxopiperidinyl, 2-oxopyrrolodinyl, 2-oxoazepinyl, azepinyl, pyrrolyl, 4-piperidonyl, pyrrolidinyl, pyrazolyl, pyrazolidinyl, imidazolyl, imidazolinyl, imidazolidinyl, pyridyl, pyrazinyl, pyrimidinyl, pyridazinyl, oxazolyl, oxazolidinyl, isoxazolyl, isoxazolidinyl, morpholinyl, thiazolyl, thiazolidinyl, isothiazolyl, quinuclidinyl, isothiazolidinyl, indolyl, quinolinyl, isoquinolinyl, chromanyl, benzimidazolyl, thiadiazoyl, benzopyranyl, benzothiazolyl, benzo[b]thiophenyl, benzo[2,3-c]1,2,5-oxadiazolyl, benzoxazolyl, furyl, tetrahydrofuryl, tetrahydropyranyl, thienyl, benzothienyl, thiamorpholinyl, thiamorpholinyl sulfoxide, thiamorpholinyl sulfone, and oxadiazolyl. Morpholino is the same as morpholinyl.
The term “halogen” or “halo” as employed herein by itself or as part of another group refers to chlorine, bromine, fluorine or iodine with fluorine being preferred.
The term “monoalkylamino” as employed herein by itself or as part of another group refers to an amino group which is substituted with one alkyl group having from 1 to 6 carbon atoms.
The term “dialkylamino” as employed herein by itself or as part of another group refers to an amino group which is substituted with two alkyl groups, each having from 1 to 6 carbon atoms.
The term “hydroxyalkyl” as employed herein refers to any of the above alkyl groups substituted by one or more hydroxyl moieties.
The term “carboxyalkyl” as employed herein refers to any of the above alkyl groups substituted by one or more carboxylic acid moieties.
The term “haloalkyl” as employed herein refers to any of the above alkyl groups substituted by one or more chlorine, bromine, fluorine or iodine with fluorine and chlorine being preferred, such as chloromethyl, iodomethyl, trifluoromethyl, 2,2,2-trifluoroethyl, and 2-chloroethyl.
The term Ahaloalkoxy≅ as used herein refers to any of the above haloalkyl groups bonded to an oxygen atom, such as trifluoromethoxy, trichloromethoxy, and the like.
The present invention is also directed to method for preparing compounds of Formula IV, comprising:

- reacting a compound of Formula V:
  
  or a salt, hydrate or solvate thereof, wherein R¹, R², R³, R⁴, R⁵, R¹⁰, R¹¹, R¹², R¹³, R¹⁴, i, j and k are as defined as above,
- with the compound of Formula VI or Formula X:
  
  or a salt, hydrate or solvate thereof, wherein R¹⁵is as defined above, to form the compound Formula IV.

The present invention is also directed to method for preparing compounds of Formula IV, comprising:

- reacting a compound of Formula V:
  
  or a salt, hydrate or solvate thereof, wherein R¹, R², R³, R⁴, R⁵, R¹⁰, R¹¹, R¹²ⁱ, R¹³, R¹⁴, i, j and k are as defined as above,
- with the compound of Formula IX:
  
  or a salt, hydrate or solvate thereof, wherein R¹⁶, R¹⁹and R²⁰are as defined above, and R³⁵is alkyl, aryl, alkylaryl or arylalkyl, followed by removal of the R³⁵containing protecting group to form the compound Formula IV.

The present invention is also directed to a method for preparing compounds of Formula IV, comprising reacting a compound of Formula V:

or a salt, hydrate or solvate thereof, wherein R¹, R², R³, R⁴, R⁵, R¹⁰, R¹¹, R¹²ⁱ, R¹³, R¹⁴, i, j and k are as defined above,

- with the compound of Formula VII:
  
  or a salt, hydrate or solvate thereof, wherein R⁶, R⁷, R⁸, R⁹, R¹⁶, m and n are as defined above, to form the compound of Formula IV.

The present invention is also directed to a method for preparing compounds of Formula IV, comprising reacting a compound of Formula VIII:

or a salt, hydrate or solvate thereof, wherein R¹, R², R³, R⁴, R⁵, R⁶, R⁷, R⁸, R⁹, R¹⁰, R¹¹, R¹², R¹³, R¹⁴, i, j, k, m and n are as defined in claim 1, with R¹⁵NCO, where R¹⁵is as defined in claim 1, to form a substituted indole compound of claim 1.
The compounds of the present invention may be prepared by the general procedures outlined in Schemes I-VII (below), where R¹, R², R³, R⁴, R⁵, R⁶, R⁷, R⁸, R⁹, R¹⁰, R¹¹, R¹², R¹³, R¹⁴, R¹⁶, n, m, i, j, X and W are as defined above. Additional R-groups, not defined above, but used throughout the schemes below are defined as indicated below:

- R¹⁷, R¹⁸, R¹⁹, R²⁰, R²⁴and R²⁵are independently hydrogen, halogen or alkyl;
- R²¹is trialkylsilyl or alkylorthoformate; preferably trimethylsilyl or (C_1-6)alkylorthoformate;
- R²²is alkyl, aryl, heteroaryl, or an aliphatic ring system;
- R²³is a protecting group such as a trialkylsilyl, such as trimethylsilyl, triisopropylsilyl; benzyl or sulfonyl;
- R²⁶is hydrogen, alkyl, aryl, heteroaryl, or an aliphatic ring system;
- R²⁷, R²⁸, R²⁹, R³⁰and R³¹are independently hydrogen, halogen, alkoxyaryl or an aliphatic ring system;
- R³⁰and R³¹are independently hydrogen, alkyl, aryl or an aliphatic ring system;
- R³²is halogen, alkyl, haloalkyl, alkoxy, alkoxyalkyl or haloalkoxy;
- R³³is halogen, alkyl, haloalkyl, alkoxy, alkoxyalkyl or haloalkoxy, and is preferably halogen;
- R³⁴and R³⁵are independently alkyl, hydroxy, alkoxy, aryl, alkylaryl or arylalkyl; and
- o and p are 0, 1 or 2.

Scheme Ia, Ib, Ic, Id and Ie outline the synthetic steps to produce compounds of the present invention where X is O and W is
In Scheme Ia, 2-chloropyridine N-oxide derivative 1 is refluxed with aminoalkyl alcohol 2 in the presence of a base, such as sodium bicarbonate, and a suitable solvent, such as tert-amyl alcohol, to give compound 3. Compound 3 is then converted to pyridinyl aminoalkyl alcohol 4 using standard reduction conditions. Preferred conditions include treating compound 3 with cyclohexene in the presence of a catalyst, such as palladium on carbon, and a solvent, such as ethanol.
In Scheme Ib, a 2-amino-5-methylpyridine analogue 5 is first protected with a tert-butyloxycarbonyl (Boc) group using conditions well known in art (Greene, T. W. and Wuts, P. G. M., Protective Groups in Organic Synthesis, 2^ndedition, John Wiley and Sons, Inc., New York (1991)), followed by treatment with an alkyl halide, such as iodomethane, in the presence of a base, such as sodium hydride, and a solvent, such as tetrahydrofuran (THF) or dimethylformamide (DMF), to give compound 6. Converting compound 6 to compound 7 is accomplished by reacting compound 6 with a base, such as lithium diisopropylamide (LDA), and diethyl carbonate in a solvent, such as tetrahydrofuran (THF). The Boc protecting group of compound 7 is removed by standard procedures well known in the art (Greene, T. W. and Wuts, P. G. M., supra), such as trifluoroacetic acid in methylene chloride. The ester is then reduced by standard conditions, such as lithium aluminum hydride (LAH) in tetrahydrofuran (THF), to give compound 8. Alternatively, compound 7 can be treated with a reducing agent, such as lithium borohydride in a solvent such as tetrahydrofuran to give compound 9.
In Scheme Ic, Compound 10 (Miller, H.; Manley, P. J., PCT Int. Appl. 2000, 40 pp. WO 00/33838) is treated with a reducing agent such as lithium borohydride, in a solvent such as tetrahydrofuran, to give compound 11.
In Scheme 1d, 3-hydroxy-6-methyl-2-nitropyridine derivative 12a is reduced under suitable conditions, such as hydrogenation in the presence of palladium catalyst, with a solvent, such as ethanol, to give compound 12b. Reaction of compound 12b (L. Savelon, et. al., Biorganic and Medicinal Chemistry, 6, 133, (1998)) with 2-haloacid chloride 12c, such as chloroacetyl chloride, in the presence of base, such as sodium bicarbonate, in suitable solvents, such as water and 2-butanone, gives compound 13. Reduction of compound 13 with suitable reagent, such as lithium aluminum hydride, in a suitable solvent, such as THF, gives compound 14. Compound 14 is protected using suitable conditions, to introduce a protecting group, such as Boc, to give compound 15 (Greene, T. W. and Wuts, P. G. M., Protective Groups in Organic Synthesis, 2^ndedition, John Wiley and Sons, Inc., New York (1991)). Compound 15 is alkylated under suitable conditions, such as deprotonation with base, such as LDA, followed by reaction with alkylating reagent, such as dialkylcarbonate, to produce compound 16. Reduction of compound 16 is achieved with suitable reducing reagent, such as lithium borohydride in a solvent such as tetrahydrofuran, to give compound 17.
In Scheme Ie, the protected indole 18 (P is protecting group), such as 5-benzyloxyindole, is reacted with a base, such as sodium hydride, and haloalkylcarboxyl ester 19, in a suitable solvent, such as N,N-dimethylformamide (DMF), to generate compound 20. The protecting group is removed by conditions well known in the art (Greene, T. W. and Wuts, P. G. M., supra), to give compound 21. For example, deprotection of benzyl ether is achieved through catalytic hydrogenation using palladium on carbon as a catalyst in a solvent, such as ethanol or tetrahydrofuran. Compound 21 is coupled to compounds 4 using a Mitsunobu coupling procedure (Mitsunobu, O., Synthesis, 1 (1981)) to give compound 22. Preferred coupling conditions include using a trialkylphosphine or triarylphosphine, such as triphenylphosphine or tri-n-butylphosphine, in a suitable solvent, such as tetrahydrofuran or methylene chloride, and an azodicarbonyl reagent, such as diethyl azodicarboxylate, diisopropyl azodicarboxylate or 1,1′-(azodicarbonyl)dipiperidine. Compound 22 is optionally converted to compound 23 by a standard procedure, such as sodium hydroxide in a solvent, such as methanol and water. Alternatively, compound 21 is coupled to compounds 8 or 11 using a Mitsunobu coupling procedure (Mitsunobu, O., Synthesis, 1 (1981)) to give compound 24. Preferred coupling conditions include using a trialkylphosphine or triarylphosphine, such as triphenylphosphine or tri-n-butylphosphine, in a suitable solvent, such as tetrahydrofuran or methylene chloride, and an azodicarbonyl reagent, such as diethyl azodicarboxylate, diisopropyl azodicarboxylate or 1,1′-(azodicarbonyl)dipiperidine. Compound 24 is optionally deprotected when Z=Boc with standard deprotection conditions (Greene, T. W. and Wuts, P. G. M., supra), followed by and optional hydrolysis using standard conditions such as sodium hydroxide in a solvent, such as methanol and water to give compound 25.
Scheme II outlines the synthetic steps to produce compounds of the present invention where X is O and W is
Compound 21 is coupled with benzyloxycarbonyl (Cbz) protected amino alcohol 26 using a Mitsunobu coupling procedure (Mitsunobu, O., Synthesis, 1 (1981)) to give compound 27. Preferred coupling conditions include using a trialkylphosphine or triarylphosphine, such as triphenylphosphine or tri-n-butylphosphine, in a suitable solvent, such as tetrahydrofuran or methylene chloride, and an azodicarbonyl reagent, such as diethyl azodicarboxylate, diisopropyl azodicarboxylate or 1,1′-(azodicarbonyl)dipiperidine. Compound 27 is deprotected using standard deprotection conditions such as hydrogenation using palladium on carbon as a catalyst in solvents such as ethanol or tetrahydrofuran, to give compound 28. Compound 28 is treated with isocyanate analogue 29 in a solvent such as acetonitrile to give compound 30. Compound 30 is optionally converted to acid 31 by a standard hydrolysis procedure such as sodium hydroxide in a solvent, such as methanol and water.
Scheme IIIa, IIIb and IIIc outline the synthetic steps to produce compounds of the present invention where X is O, and W is
In Scheme IIIa, aryl halides 32 are reacted with protected acetylenes 33, such as trimethylsilylacetylenes or trialkyloxypropynes under cross coupling conditions with suitable reagents, such as palladium (II) and copper iodide, in the presence of base, such as triethylamine, to give protected arylacetylene compounds 34 (Sonogashira, K., Tetrahedron Lett. 1975, 50, 4467-70). Removal of the trimethylsilyl group of compound 34 is achieved under various conditions, such as tetrabutylammonium fluoride or base, to give compound 35 (Greene, T. W. and Wuts, P. G. M., Protective Groups in Organic Synthesis, 2^ndedition, John Wiley and Sons, Inc., New York (1991)). Treatment of compound 35 with a suitable reagent, such as alkyl haloformate, in the presence of base, such as LDA, or butyllithium, gives compound 36. Alternatively, the aryl triethoxypropyne 34 can be treated with a suitable acid, such as p-toluenesulfonic acid, to give compound 36.
In Scheme IIIb, aliphatic acetylene 37 or aromatic acetylene 37 (synthesized using methodology describe in Scheme IIIa) is treated with alkylchloroformate in the presence of a catalyst such as carbonylchlorobis-(triphenylphosphine)-rhodium(I), in a solvent such as toluene, to give compound 38.
In Scheme IIIc, aliphatic or aromatic aldehyde 39 is treated with suitable reagents, such carbontetrabromide and triphenylphosphine, to give compound 40. Treatment of the compound 40 with suitable base, such as n-butyllithium, gives compound 41. Reaction of compound 41 with suitable base, such as LDA, or n-butyllithium (Corey, E. J.; Fuchs, P. L., Tetrahedron Lett. (1972), (36), 3769-72), followed by alkyl haloformate, such as ethyl chloroformate, generates compound 42.
In Scheme IIId, aromatic or aliphatic ketones 43 are treated with base, such as sodium hydride, in a solvent such as tetrahydrofuran, and dialkylcarbonate or alkylchloroformate to give compound 44. Compound 44 is then treated with triphenylphosphine oxide and trifluoromethanesulfonate anhydride in the presence of a base, such as triethylamine to give compound 42 (Hendrickson, J., Synthesis, 1989, 217).
In Scheme IIIe, compound 9 or 11 or 17 is coupled with a 3-methyl-4-nitro-phenol derivative 45 using a Mitsunobu coupling procedure (Mitsunobu, O., Synthesis, 1 (1981)) to give compounds 46. Preferred coupling conditions include using a trialkylphosphine or triarylphosphine, such as triphenylphosphine or tri-n-butylphosphine, in a suitable solvent, such as tetrahydrofuran or methylene chloride, and an azodicarbonyl reagent, such as diethyl azodicarboxylate, diisopropyl azodicarboxylate or 1,1′-(azodicarbonyl)dipiperidine. Compound 46 can be treated with pyrrolidine and dimethoxymethyl dimethylamine to give the corresponding enamine, followed by standard reduction conditions such as hydrogenation in the presence of a catalyst, such as palladium on carbon, and a solvent such as ethanol, to give compound 47 (Batcho, A., Batcho, Andrew D.; Leimgruber, Willy., Org. Synth. 1985, 63, 214-25). Compound 47 is then reacted with an appropriate substituted propiolate 36 or 42, in the presence of a base, such as cesium fluoride or tetrabutylammonium fluoride, in a solvent such as THF or DMF, to give compound 48. Alternatively, compound 47 is treated with substituted vinylhalide ester 38 using a catalyst such as carbonylchlorobis-(triphenylphosphine)-rhodium(I) in a solvent such as toluene to give compound 48.
Compound 48 is then optionally reduced through treatment such as hydrogenation, in the presence of a catalyst, such as palladium on carbon, followed by Boc removal which can be carried out by deprotection conditions such as heating the neat compound to 180° C. to give compound 49a or 49b. Compound 49a or 49b can then optionally be hydrolyzed in the presence of a base, such as potassium hydroxide in a solvent such as methanol and water, to give compound 50a or 50b.
Scheme IVa, IVb, IVc and IVd outline the synthetic steps to produce compounds of the present invention where X is C, and W is
In Scheme IVa, 5-haloindole derivative 51 is protected under standard protection conditions with triisopropylsilylchloride, in the presence of a base, such as lithium hexamethyldisilazane, to give protected compound 52. Compound 52 is coupled with cyanoalkyl zinc halide 53, such as 3-cyanopropyl zinc bromide, in the presence of a catalyst, such as tetrakis(triphenylphosphine)palladium(0), to afford compound 54. Compound 54 is treated under suitable conditions, such as alkyl magnesium halides, followed by quenching with water to give compound 55. Finally, the compound 55 is condensed with substituted 2-amino-pyridine-3-carbaldehyde 56, in the presence of a base, such as L-proline, in a solvent, such as ethanol, to give a mixture of compound 57 and 58.
In Scheme IVb, compound 58 is treated with substituted propynoic acid ester 36 or 42, such as phenyl propynoic acid ethyl ester, in the presence of a base, such as tetrabutylammonium fluoride or cesium fluoride, in a solvent such as tetrahydrofuran, to give compound 59 as an E/Z isomeric mixture. Compound 59 is reduced under standard reduction conditions such as hydrogenation, in the presence of a catalyst, such as palladium on carbon, with a solvent, such as methanol, to give compound 60. Optional hydrolysis of compound 60 under suitable conditions, such as aqueous lithium hydroxide or sodium hydroxide, in a suitable solvent, such as methanol or THF, gives compound 61.
In Scheme IVc, compound 57 is deprotected under suitable conditions with reagents, such as tetrabutylammonium fluoride, in a solvent, such as tetrahydrofuran, to give compound 62. Compound 62 is then treated with alkyl halide 19 such as 3-bromo-propionic acid ethyl ester, in the presence of a base, such as sodium hydride, in a solvent, such as DMF, to give compound 63. Compound 63 is reduced under standard reduction conditions such as hydrogenation, in the presence of a catalyst, such as palladium on carbon, with a solvent, such as methanol or ethyl acetate, to give compound 64. Optional hydrolysis of compound 64 is done under suitable conditions, such as aqueous lithium hydroxide or sodium hydroxide, in a suitable solvent, such as methanol or THF, to give compound 65.
In Scheme IVd, compound 57 is treated with substituted propynoic acid ester 36 or 42, such as phenyl propynoic acid ethyl ester, in the presence of a base, such as tetrabutylammonium fluoride or cesium fluoride, in a solvent such as tetrahydrofuran, to give compound 66 as an E/Z isomeric mixture. Compound 66 is reduced under standard reduction conditions such as hydrogenation, in the presence of a catalyst, such as palladium on carbon, with a solvent, such as methanol or ethyl acetate, to give a mixture of compound 67 and 68. Without separation, optional hydrolysis of the mixture of compounds 67 and 68 under basic conditions, such as aqueous lithium hydroxide or sodium hydroxide solution in THF or methanol, to give compound 69 as the major product, with compound 70 as the minor product.
Scheme V outline the synthetic steps to produce compounds of the present invention where X is O and W is
In Scheme V, protected 5-hydroxylindole compound 18 is treated with substituted propynoic acid ester 36 or 42, such as phenyl propynoic acid ethyl ester, in the presence of a base, such as tetrabutylammonium fluoride or cesium fluoride, in solvent such as tetrahydrofuran, to give compound 71 as an E/Z isomeric mixture. Compound 71 is reduced under standard reduction conditions such as hydrogenation, in the presence of a catalyst, such as palladium on carbon, with a solvent, such as methanol or ethyl acetate, to give compound 72. Compound 72 is coupled with compound II or 17 using a Mitsunobu coupling procedure (Mitsunobu, O., Synthesis, 1 (1981)) to give compound 73. Preferred coupling conditions include using a trialkylphosphine or triarylphosphine, such as triphenylphosphine or tri-n-butylphosphine, in a suitable solvent, such as tetrahydrofuran or methylene chloride, and an azodicarbonyl reagent, such as diethyl azodicarboxylate, diisopropyl azodicarboxylate or 1,1′-(azodicarbonyl)dipiperidine. Deprotection of compound 73 is carried out with copper (I) trifluoremethanesulfonate, in a solvent, such as DMF in toluene at 200° C. to give compound 74. Optional hydrolysis of compound 74 under basic conditions, such as aqueous lithium hydroxide or sodium hydroxide in THF or methanol, gives compound 75.
Scheme VI outline the synthetic steps to produce compounds of the present invention where X is NR, R6 and R7 are combined to form a carbonyl, and W is
In Scheme VI, 5-nitroindole derivative 76 is treated with alkyl halides 19 in the presence of base, such as sodium hydride, to give compound 77. Compound 77 is reduced under standard conditions, such as hydrogenation with a catalyst, such as palladium on activated carbon, with a suitable solvent, such as ethanol or methanol, to compound 78. Compound 10 is hydrolyzed under suitable conditions, such as sodium hydroxide to give the free acid, followed by Boc deprotection which is carried out using standard deprotection conditions (T. W. Greene, Protective groups in organic synthesis, 1999 John Wiley & Sons, Inc.) to give compound 79. Compound 79 is then coupled with compound 78 under typical amide coupling conditions, such as benzotriazol-1-yloxy-tris(dimethylamino)phosphonium hexafluorophophate, diisopropylethylamine, and dimethylformamide, to give compound 80. Optionally, compound 80 is hydrolyzed under typical conditions, such as sodium hydroxide, with suitable solvent, such as water and methanol, to give compound 81.
Scheme VII outline the synthetic steps to produce compounds of the present invention where X is O, D is O, v is 1, and W is
In Scheme VII, 3-methyl-4-nitrophenol derivative 45 is coupled to an aliphatic alcohol 82 using standard Mitsunobu coupling procedure (Mitsunobu, O., Synthesis, 1 (1981)) to give compound 83. Preferred coupling conditions include using a trialkylphosphine or triarylphosphine, such as triphenylphosphine or tri-n-butylphosphine, in a suitable solvent, such as tetrahydrofuran or methylene chloride, and an azodicarbonyl reagent, such as diethyl azodicarboxylate, diisopropyl azodicarboxylate or 1,1′-(azodicarbonyl)dipiperidine. Compound 83 is treated with pyrrolidine and dimethoxymethyl dimethylamine analogues to give the corresponding enamine 84, followed by standard reduction conditions such as hydrogenation in the presence of a catalyst, such as palladium on carbon, and a solvent such as ethanol, to give compound 85 (Batcho, A., Batcho, Andrew D.; Leimgruber, Willy., Org. Synth. 1985, 63, 214-25). Compound 85 is reacted with a substituted propiolate 36 or 42, in the presence of a weak base to yield the corresponding alkene 86, as an E/Z mixture. Preferred conditions include the treatment of compound 85 with tetrabutylammonium fluoride in tetrahydrofuran. Compound 86 is deprotected and reduced using standard conditions, such as hydrogenation, using a catalyst such as palladium on carbon, in a suitable solvent, such as ethanol, to give compound 87. Compound 87 is treated with N-hydroxyphthalimide using standard Mitsunobu coupling procedure (Mitsunobu, O., Synthesis, 1, 1981) to give compound 88. Preferred coupling conditions include using a trialkylphosphine or triarylphosphine, such as triphenylphosphine or tri-n-butylphosphine, in a suitable solvent, such as tetrahydrofuran or methylene chloride, and an azodicarbonyl reagent, such as diethyl azodicarboxylate, diisopropyl azodicarboxylate or 1,1′-(azodicarbonyl)dipiperidine. Deprotection of compound 88 is carried out in the presence of a primary amine, preferred conditions include the use of methylamine in tetrahydrofuran, to give compound 89. Alkylation of compound 89 with a corresponding pyrazole, such as 1H-pyrazole-1-carboxamide hydrochloride or 2-(3,5-dimethylpyrazolyl)-4,5-dihydroimidizole hydrobromide in methanol gives compound 90. Optional hydrolysis of the compound 90 using lithium hydroxide in the presence of water afforded compound 91.
Compounds of the present invention can be tested for the ability to inhibit or antagonize α_vβ₃or α_vβ₅cell surface receptors by assays known to those of ordinary skill in the art. Such assays are described in Example 58 herein.
The present invention also provides a method of treating α_vβ₃integrin- or α_vβ₅integrin-mediated conditions by selectively inhibiting or antagonizing α_vβ₃and α_vβ₅cell surface receptors, which method comprises administering a therapeutically effective amount of a compound selected from the class of compounds depicted by Formula IV, wherein one or more compounds of Formula IV is administered in association with one or more non-toxic, pharmaceutically acceptable carriers and/or diluents and/or adjuvants and if desired other active ingredients.
More specifically, the present invention provides a method for inhibition of the α_vβ₃cell surface receptor. Most preferably, the present invention provides a method for inhibiting bone resorption, treating osteoporosis, inhibiting humoral hypercalcemia of malignancy, treating Paget=s disease, inhibiting tumor metastasis, inhibiting neoplasia (solid tumor growth), inhibiting angiogenesis including tumor angiogenesis, treating diabetic retinopathy, age-related macular degeneration, retinopathy of prematurity and other neo-vascular eye diseases, inhibiting arthritis, psoriasis and periodontal disease, and inhibiting smooth muscle cell migration including neointimal hyperplasia and restenosis.
The present invention also provides a method for inhibition of the α_vβ₅cell surface receptor. Most preferably, the present invention provides a method for inhibiting angiogenesis associated with pathological conditions such as inflammatory disorders such as immune and non-immune inflammation, chronic articular rheumatism and psoriasis, disorders associated with inappropriate or inopportune invasion of vessels such as restenosis, capillary proliferation in atherosclerotic plaques and osteoporosis, and cancer associated disorders, such as solid tumors, solid tumor metastases, angiofibromas, retrolental fibroplasia, hemangiomas, Kaposi sarcoma and similar cancers which require neovascularization to support tumor growth. The present invention also provides a method for treating eye diseases characterized by angiogenesis, such as diabetic retinopathy, age-related macular degeneration, presumed ocular histoplasmosis, retinopathy of prematurity, and neovascular glaucoma.
The compounds of the present invention are useful in treating cancer, including tumor growth, metastasis and angiogenesis. For example, compounds of the present invention can be employed to treat breast cancer and prostate cancer.
The compounds of the present invention are also useful in the treatment of sickle cell anemia. α_vβ₃integrin has recently been implicated in the mechanism of adhesion of sickled red blood cells (RBCs) to vascular structures within the circulatory system of those suffering from sickle cell anemia. Adhesion of RBC=s is responsible for the reoccurring episodes of painful vasocclusive crisis and multiple organ damage. (Kaul et al., Blood 95(2):368-373 (2000)). Monoclonal antibodies which bind to α_vβ₃have been shown to inhibit the adhesion of sickled RBCs in the ex vivo mesocecum vasculature of the rat. By blocking α_vβ₃integrin which assists in adhesion of sickled cells to vascular components, a reduction in the harmful affects of sickle cell anemia is realized.
The compounds of the present invention are also useful in the treatment of central nervous system (CNS) related disorders. Treatment of such CNS related disorders includes, but is not limited to: treating or preventing neuronal loss associated with stroke, ischemia, CNS trauma, hypoglycemia, and surgery, as well as treating neurodegenerative diseases including Alzheimer's disease, and Parkinson's disease, treating or preventing the adverse consequences of the overstimulation of the excitatory amino acids, as well as treating schizophrenia, anxiety, convulsions, chronic pain, psychosis, including anesthesia, and preventing opiate tolerance.
Studies have shown that there is a correlation between the activity of ∀4 integrin and the establishment of inflammatory lesions in the CNS. Brocke, S. et al., Proc. Natl. Acad. Sci. USA 96:6896-6901 (1999). Specifically, antibodies directed against CD44 and ∀4 integrin could interfere in several ways with the establishment of inflammatory lesions in the CNS and thus prevent experimental autoimmune encephalomyelitis (EAE), an inflammatory disease of the CNS similar to multiple sclerosis. Brocke at 6899.
Relton and co-workers have also shown that inhibition of ∀4 integrin activity protects the brain against ischemic brain injury, thereby implicating ∀4 integrin as a factor in acute brain injury. Relton, et al., Stroke 32(1):199-205 (2001).
The compounds of the present invention may be administered in an effective amount within the dosage range of about 0.01 mg/kg to about 300 mg/kg, preferably between 1.0 mg/kg to 100 mg/kg body weight. Compounds of the present invention may be administered in a single daily dose, or the total daily dosage may be administered in divided doses of two, three or four times daily.
The pharmaceutical compositions of the present invention can be administered to any animal that can experience the beneficial effects of the compounds of the invention. Foremost among such animals are humans, although the invention is not intended to be so limited.
The pharmaceutical compositions of the present invention can be administered by any means that achieve their intended purpose. For example, administration can be by parenteral, subcutaneous, intravenous, intramuscular, intraperitoneal, transdermal, buccal, or ocular routes. Alternatively, or concurrently, administration can be by the oral route. The dosage administered will be dependent upon the age, health, and weight of the recipient, kind of concurrent treatment, if any, frequency of treatment, and the nature of the effect desired.
In addition to the pharmacologically active compounds, the pharmaceutical preparations of the compounds can contain suitable pharmaceutically acceptable carriers comprising excipients and auxiliaries that facilitate processing of the active compounds into preparations that can be used pharmaceutically. The pharmaceutical preparations of the present invention are manufactured in a manner that is, itself, known, for example, by means of conventional mixing, granulating, dragee-making, dissolving, or lyophilizing processes. Thus, pharmaceutical preparations for oral use can be obtained by combining the active compounds with solid excipients, optionally grinding the resulting mixture and processing the mixture of granules, after adding suitable auxiliaries, if desired or necessary, to obtain tablets or dragee cores.
Suitable excipients are, in particular, fillers such as saccharides, for example, lactose or sucrose, mannitol or sorbitol, cellulose preparations and/or calcium phosphates, for example, tricalcium phosphate or calcium hydrogen phosphate, as well as binders, such as starch paste, using, for example, maize starch, wheat starch, rice starch, potato starch, gelatin, tragacanth, methyl cellulose, hydroxypropylmethylcellulose, sodium carboxymethylcellulose, and/or polyvinyl pyrrolidone. If desired, disintegrating agents can be added, such as the above-mentioned starches and also carboxymethyl-starch, cross-linked polyvinyl pyrrolidone, agar, or alginic acid or a salt thereof, such as sodium alginate. Auxiliaries are, above all, flow-regulating agents and lubricants, for example silica, talc, stearic acid or salts thereof, such as magnesium stearate or calcium stearate, and/or polyethylene glycol. Dragee cores are provided with suitable coatings, that, if desired, are resistant to gastric juices. For this purpose, concentrated saccharide solutions can be used, which may optionally contain gum arabic, talc, polyvinyl pyrrolidone, polyethylene glycol, and/or titanium dioxide, lacquer solutions and suitable organic solvents or solvent mixtures. In order to produce coatings resistant to gastric juices, solutions of suitable cellulose preparations, such as acetylcellulose phthalate or hydroxypropylmethylcellulose phthalate, are used. Dye stuffs or pigments can be added to the tablets or dragee coatings, for example, for identification or in order to characterize combinations of active compound doses.
Other pharmaceutical preparations that can be used orally include push-fit capsules made of gelatin, as well as soft, sealed capsules made of gelatin and a plasticizer such as glycerol or sorbitol. The push-fit capsules can contain the active compounds in the form of granules that may be mixed with fillers such as lactose, binders such as starches, and/or lubricants such as talc or magnesium stearate and, optionally, stabilizers. In soft capsules, the active compounds are preferably dissolved or suspended in suitable liquids such as fatty oils or liquid paraffin. In addition, stabilizers may be added.
Suitable formulations for parenteral administration include aqueous solutions of the active compounds in water-soluble form, for example water-soluble salts and alkaline solutions. Alkaline salts can include ammonium salts prepared, for example, with Tris, choline hydroxide, bis-Tris propane, N-methylglucamine, or arginine. In addition, suspensions of the active compounds as appropriate oily injection suspensions can be administered. Suitable lipophilic solvents or vehicles include fatty oils, for example, sesame oil, or synthetic fatty acid esters, for example, ethyl oleate or triglycerides or polyethylene glycol-400 (the compounds are soluble in PEG-400). Aqueous injection suspensions can contain substances that increase the viscosity of the suspension, for example sodium carboxymethyl cellulose, sorbitol, and/or dextran. Optionally, the suspension may also contain stabilizers.
The compounds of the present invention may be administered to the eye in animals and humans as a drop, or within ointments, gels, liposomes, or biocompatible polymer discs, pellets or carried within contact lenses. The intraocular composition may also contain a physiologically compatible ophthalmic vehicle as those skilled in the art can select using conventional criteria. The vehicles may be selected from the known ophthalmic vehicles which include but are not limited to water, polyethers such polyethylene glycol 400, polyvinyls such as polyvinyl alcohol, povidone, cellulose derivatives such as carboxymethylcellulose, methylcellulose and hydroxypropyl methylcellulose, petroleumn derivatives such as mineral oil and white petrolatum, animal fats such as lanolin, vegetable fats such as peanut oil, polymers of acrylic acid such as carboxylpolymethylene gel, polysaccharides such as dextrans and glycosaminoglycans such as sodium chloride and potassium, chloride, zinc chloride and buffer such as sodium bicarbonate or sodium lactate. High molecular weight molecules can also be used. Physiologically compatible preservatives which do not inactivate the compounds of the present invention in the composition include alcohols such as chlorobutanol, benzalknonium chloride and EDTA, or any other appropriate preservative known to those skilled in the art.
The following examples are illustrative, but not limiting, of the method and compositions of the present invention. Other suitable modifications and adaptations of the variety of conditions and parameters normally encountered and obvious to those skilled in the art are within the spirit and scope of the invention.

EXAMPLE 1

3-{5-[3-(2-Pyridylamino)propoxy]indolyl}propanoic acid ammonium salt

a). 2-(3-Hydroxypropyl)aminopyridine N-oxide

A mixture of 2-chloropyridine-N-oxide hydrochloride (3.32 g, 20 mmol), 3-amino-1-propanol (3.06 mL, 40 mmol), NaHCO₃(8.4 g, 100 mmol) in tert-amyl alcohol (20 mL) was heated to reflux. After stirring overnight, the reaction mixture was cooled, diluted with methylene chloride (100 mL), and suction filtered to remove the insoluble materials. The filtrate was concentrated and reconcentrated from methylene chloride twice. The residue was recrystallized from ethyl acetate and hexane, collected by filtration, washed with ethyl acetate, and dried under high vacuum to give the title compound as a pale yellow solid (3.2 g, 95%). ¹H-NMR (400 MHz, CDCl₃) * 8.07 (d, J=6.5 Hz, 1H), 7.32 (br s, 1H), 7.21 (t, J=8.6 Hz, 1H), 6.64 (d, J=8.5 Hz, 1H), 6.53 (t, J=6.7 Hz, 1H), 3.75 (t, J=5.8 Hz, 2H), 3.47 (q, J=6.2 Hz, 2H), 1.86 (t, J=6.0 Hz, 2H).

b). 2-(3-Hydroxypropyl)aminopyridine

A mixture of 2-(3-hydroxypropyl)aminopyridine N-oxide (3.0 g, 17.9 mmol), as prepared in the preceding step, cyclohexene (10 mL, 100 mmol), and 10% palladium(0) on carbon (300 mg) in ethanol (50 mL) was heated to reflux. After two days, the reaction mixture was cooled. The catalyst was removed by filtration through Celite and the filtrate was concentrated. The residue was purified by flash column chromatography (silica gel, 5% methanol in methylene chloride) to give the title compound as a colorless oil (2.4 g, 88%). ¹H-NMR (400 MHz, CDCl₃) * 8.02 (d, J=5.0 Hz, 1H), 7.37 (t, J=7.8 Hz, 1H), 6.54 (d, J=6.0 Hz, 1H), 6.39 (t, J=8.0 Hz, 1H), 4.69 (br s, 2H), 3.65 (t, J=5.5 Hz, 2H), 3.53 (q, J=5.9 Hz, 2H), 1.77 (t, J=5.6 Hz, 2H).

c). Ethyl 3-(5-benzyloxy)indolyl]propanoate

A solution of 5-benzyloxyindole (1.30 g, 5.82 mmol) was dissolved in anhydrous N,N-dimethylformamide (25 mL) under nitrogen and treated with a 60% suspension of sodium hydride in mineral oil (0.60 g, 15 mmol). After stirring 1 hour (Ah≅) at ambient temperature, the reaction was treated with ethyl 3-bromopropionate (1.00 mL, 6.96 mmol) and stirred an additional 18 h. The reaction was then treated with additional sodium hydride (0.3 g, 7.5 mmol), stirred 2 more hours and the solvent removed in vacuo. The crude product was dissolved in methylene chloride, washed with 10% aqueous HCl, water, and brine, dried over anhydrous sodium sulfate, and filtered. The filtrate was evaporated and the residue purified by flash column chromatography (1:1 methylene chloride:ethyl acetate eluant) giving the title compound as a yellow oil (0.96 g, 51%). ¹H NMR (400 MHz, CDCl₃) * 7.47 (br d, 2H, J=7.2 Hz), 7.37 (m, 2H), 7.32 (m, 1H), 7.24 (br d, 1H, J=8.8 Hz), 7.15 (d, 1H, J=2.4 Hz), 7.10 (m, 1H), 6.96 (dd, 1H, J=8.8 Hz, 2.4 Hz), 6.38 (m, 1H), 5.09 (s, 2H), 4.44 (t, 2H, J=6.9 Hz), 4.21 (q, 2H, J=7.1 Hz), 2.92 (t, 2H, J=6.9 Hz), 1.26 (m, 3H).

d). Ethyl 3-(5-hydroxyindolyl)propanoate

A solution of the product of the preceding step (0.94 g, 2.90 mmol) and 10% palladium(0) on carbon (97 mg) in reagent ethanol (40 mL) was stirred under hydrogen at ambient pressure and temperature for 18 h. The reaction was filtered over Celite, and the evaporated filtrate purified by flash column chromatography (10% ethyl acetate in methylene chloride eluant) giving the title compound as a colorless oil (0.36 g, 53%). ¹H NMR (400 MHz, CDCl₃) * 7.18 (d, 1H, J=8.7 Hz), 7.10 (d, 1H, J=3.0 Hz), 7.01 (d, 1H, J=1.9 Hz), 6.78 (dd, 1H, J=8.7 Hz, 2.2 Hz), 6.34 (d, 1H, J=3.0 Hz), 4.86 (s, 1H), 4.43 (t, 2H, J=6.9 Hz), 4.22 (q, 2H, J=7.1 Hz), 2.92 (t, 2H, J=6.9 Hz), 1.27 (t, 3H, J=7.1 Hz).

e). Ethyl 3-{5-[3-(2-pyridylamino)propoxy]indolyl}propanoate

A solution of the product of the preceding step (0.35 g, 1.51 mmol) and the product of Example 1, Step b (0.24 g, 1.58 mmol) in anhydrous tetrahydrofuran (25 mL) was treated with tri-n-butylphosphine (0.43 mL, 1.72 mmol) and 1,1-(azodicarbonyl)dipiperidine (0.43 g, 1.70 mmol) at ambient temperature. After 18 h the reaction was concentrated in vacuo and the crude product purified by flash column:chromatography (1:1 methylene chloride:ethyl acetate eluant) giving the title compound as a yellow oil (0.33 g, 60%). ¹H NMR (400 MHz, CDCl₃) * 8.08 (dd, 1H, J=5 Hz, 1 Hz), 7.40 (m, 1H), 7.24 (d, 1H, J=8.8 Hz), 7.11 (d, 1H, J=3.1 Hz), 7.09 (d, 1H, J=2.4 Hz), 6.89 (dd, 1H, J=8.8 Hz, 2.4 Hz), 6.55 (m, 1H), 6.41 (d, 1H, J=8.4 Hz), 6.39 (d, 1H, J=3.0 Hz), 4.76 (br m, 1H), 4.45 (t, 2H, J=6.9 Hz), 4.22 (q, 2H, J=7.1 Hz), 4.12 (m, 2H), 3.53 (dd, 2H, J=12.6 Hz, 6.5 Hz), 2.93 (t, 2H, J=6.9 Hz), 2.12 (pentet, 2H, J=6 Hz), 1.27 (m, 3H).

f). 3-{5-[3-(2-Pyridylamino)propoxy]indolyl}propanoic acid ammonium salt

The product of the preceding step (0.33 g, 0.90 mmol) was dissolved in methanol (10 mL) and treated with 1 N aqueous LiOH (2 mL) at ambient temperature. After 18 h the reaction was acidified with 10% aqueous HCl, concentrated in vacuo, and the crude product purified by flash column chromatography (15% methanol in methylene chloride eluant) giving a very hygroscopic solid. This was dissolved in a mixture of methylene chloride and methanol (saturated with ammonia gas), filtered, and the filtrate concentrated in vacuo giving the title compound as a stable, pale yellow solid (0.14 g, 42%). ¹H NMR (400 MHz, DMSO-d₆): * 7.92 (m, 1H), 7.59 (m, 1H), 7.37 (d, 1H, J=8.9 Hz), 7.28 (d, 1H, J=3.1 Hz), 7.04 (d, 1H, J=2.3 Hz), 6.78 (dd, 1H, J=8.9 Hz, 2.3 Hz), 6.75 (d, 1H, J=9.7 Hz), 6.63 (br t, 1H, J=6.3 Hz), 4.34 (t, 2H, J=6.8 Hz), 4.05 (t, 2H, J=6.2 Hz), 3.45 (dd, 2H, J=12.5 Hz, 6.6 Hz), 2.71 (t, 2H, J=6.8 Hz), 2.02 (pentet, 2H, J=6.5 Hz). Mass spectrum (LCMS, ESI pos.) Calcd. for C₁₉H₂₁N₃O₃: 339.4 (M+H). Found: 340.1.

EXAMPLE 2

3-{5-[3-(2-Pyridylamino)propoxy]indolyl}acetic acid ammonium salt

a). Methyl 2-(5-benzyloxyindolyl)acetate

5-Benzyloxyindole (0.80 g, 3.58 mmol) was dissolved in anhydrous N,N-dimethylformamide (20 mL) and treated with 60% sodium hydride in mineral oil (0.36 g, 9.00 mmol) at ambient temperature. After 2 h, ethyl bromoacetate (0.45 mL, 4.06 mmol) was added, the reaction stirred for 6 h, and additional sodium hydride (0.36 g, 9.00 mmol) was added. The reaction stirred for 3 days, the N,N-dimethylformamide was removed in vacuo, and the residue was dissolved in methylene chloride. The resulting solution washed with 10% aqueous HCl, water, and brine, dried over anhydrous sodium sulfate, and filtered. The evaporated filtrate was then dissolved in N,N-dimethylformamide (20 mL) and treated with cesium carbonate (1.57 g, 4.80 mmol) and iodomethane (0.30 mL, 3.75 mmol) at ambient temperature for 18 h. The reaction was concentrated in vacuo, the crude product dissolved in methylene chloride, and the solution washed with saturated aqueous bicarbonate, water, and brine, dried over sodium sulfate, and filtered. The evaporate filtrate then gave the title compound (0.93 g, 84%) as an oily orange solid. ¹H NMR (400 MHz, CDCl₃): * 7.46 (br d, 2H, J=7.3 Hz), 7.38 (m, 2H), 7.31 (d, 1H, J=7.2 Hz), 7.17 (d, 1H, J=2.4 Hz), 7.14 (d, 1H, J=8.8 Hz), 7.06 (d, 1H, J=3.1 Hz), 6.96 (dd, 1H, J=8.9 Hz, 2.4 Hz), 6.46 (d, 1H, J=3.1 Hz), 5.10 (s, 2H), 4.79 (s, 2H), 4.20 (q, 2H, J=7.1 Hz), 1.25 (t, 3H, J=7.1 Hz).

b). Methyl 2-(5-hydroxyindolyl)acetate

A solution of the product of the preceding step (0.92 g, 2.97 mmol) and 10% palladium(0) on carbon (94 mg) in reagent ethanol (40 mL) was stirred under hydrogen at ambient pressure and temperature for 18 h. The reaction was filtered over Celite, and the evaporated filtrate dissolved in reagent ethanol (50 mL) and hydrogenated again as above over 10% palladium(0) on carbon (170 mg) for 24 h. The reaction was again filtered over Celite, the evaporated filtrate dissolved in methylene chloride, poured over a short bed of silica gel, and eluted with 1:1 methylene chloride:ethyl acetate. The eluate was then concentrated in vacuo giving the title compound as a light brown oil (0.61 g, 93%). ¹H NMR (400 MHz, CDCl₃): * 7.09 (d, 1H, J=8.7 Hz), 7.06 (d, 1H, J=3.1 Hz), 7.02 (d, 1H, J=2.4 Hz), 6.78 (dd, 1H, J=8.7 Hz, 2.4 Hz), 6.42 (m, 1H), 4.79 (s, 2H), 4.21 (q, 2H, J=7.1 Hz), 1.25 (m, 3H).

c). Methyl 2-{5-[3-(2-pyridylamino)propoxy]indolyl}acetate

A solution of the product of the preceding step (0.31 g, 1.41 mmol) and the product of Example 1, Step b (0.23 g, 1.48 mmol) in anhydrous tetrahydrofuran (30 mL) was treated with tri-n-butylphosphine (0.41 mL, 1.64 mmol) and 1,1-(azodicarbonyl)dipiperidine (0.41 g, 1.63 mmol) at ambient temperature. After 18 h the reaction was concentrated in vacuo and the crude product purified by flash column:chromatography (1:1 methylene chloride:ethyl acetate eluant) giving the title compound (0.24 g, 48%) as a gold oil. ¹H NMR (400 MHz, CDCl₃): * 8.09 (m, 1H), 7.39 (ddd, 1H, J=8.3 Hz, 7.2 Hz, 1.9 Hz), 7.13 (d, 1H, J=8.9 Hz), 7.10 (d, 1H, J=2.3 Hz), 7.06 (d, 1H, J=3.1 Hz), 6.89 (dd, 1H, J=8.9 Hz, 2.3 Hz), 6.55 (ddd, 1H, J=7.1 Hz, 5.1 Hz, 0.8 Hz), 6.46 (dd, 1H, J=3.1 Hz, 0.6 Hz), 6.41 (d, 1H, J=8.4 Hz), 4.78 (m, 3H), 4.20 (q, 2H, J=7.1 Hz), 4.13 (m, 2H), 3.52 (dd, 2H, J=12.6 Hz, 6.5 Hz), 2.12 (pentet, 2H, J=6.3 Hz), 2.04 (s, 3H), 1.26 (m, 3H).

d). 2-{5-[3-(2-Pyridylamino)propoxy]indolyl}acetic acid ammonium salt

The product of the preceding step (0.23 g, 0.65 mmol) was dissolved in methanol (15 mL) and treated with 1 N aqueous LiOH (2 mL) at ambient temperature. After 3 days, the reaction was acidified with 10% aqueous HCl, concentrated in vacuo. The crude product purified by flash column chromatography (25% methanol in methylene chloride saturated with ammonia gas as eluant), the concentrated fractions treated with a few drops of 4 N HCl in dioxane, and concentrated in vacuo giving a yellow gum. This was dissolved in a mixture of methylene chloride and methanol (saturated with ammonia gas), filtered, and the filtrate concentrated in vacuo giving the title compound as a yellow solid (0.16 g, 70%). ¹H NMR (400 MHz, DMSO-d₆): * 7.33 (m, 1H), 7.21 (d, 1H, J=2.9 Hz), 7.18 (d, 1H, J=8.8 Hz), 7.02 (d, 1H, J=2.2 Hz), 6.73 (dd, 1H, J=8.8 Hz, 2.1 Hz), 6.56 (m, 1H), 6.45 (m, 2H), 6.26 (d, 1H, J=2.8 Hz), 4.65 (s, 2H), 4.03 (t, 2H, J=6.3 Hz), 3.37 (m, 2H), 1.96 (m, 2H). Mass spectrum (LCMS, ESI pos.) Calcd. for C₁₈H₁₉N₃O₃: 326.4 (M+H). Found: 326.1.

EXAMPLE 3

3-{2-Methyl-5-[3-(2-pyridylamino)propoxy]indolyl}propanoic acid sodium salt

a). 3-(5-Methoxy-2-methylindolyl)propanoic acid

5-Methoxy-2-methylindole (0.50 g, 3.10 mmol) was dissolved in anhydrous N,N-dimethylformamide (25 mL) and treated with 60% sodium hydride in mineral oil (0.19 g, 4.70 mmol) at ambient temperature for 2 h. Ethyl 3-bromopropionate (0.60 mL, 4.20 mmol) was added, the reaction stirred for 3.5 h, treated with additional sodium hydride (0.20 g, 4.88 mmol), and stirred another 24 h. After concentration in vacuo, the crude product was dissolved in methylene chloride, the solution washed with dilute aqueous HCl and brine, dried over anhydrous sodium sulfate, and filtered. The evaporated filtrate was purified by flash column chromatography (1:1 hexane:ethyl acetate as eluant) giving the title compound as a yellow-orange solid (0.56 g, 77%). ¹H NMR (400 MHz, CDCl₃): * 7.16 (d, 1H, J=8.8 Hz), 7.00 (d, 1H, J=2.4 Hz), 6.80 (dd, 1H, J=8.8 Hz, 2.4 Hz), 6.17 (s, 1H), 4.36 (t, 2H, J=7.4 Hz), 3.83 (s, 3H), 2.78 (t, 2H, J=7.4 Hz), 2.41 (s, 3H).

b). 3-(5-Hydroxy-2-methylindolyl)propanoic acid

The product of the preceding step (0.55 g, 2.36 mmol) was dissolved in anhydrous methylene chloride (25 mL) under nitrogen, cooled to −78° C., and treated with 1 N boron tribromide in methylene chloride (4.8 mL, 4.8 mmol). The reaction was allowed to slowly warm to ambient temperature over 18 h, quenched with excess water, and the phases separated. The organic phase washed with brine, dried over sodium sulfate, filtered, and the evaporated filtrate purified by flash column chromatography (10% methanol in methylene chloride as eluant) giving the title compound as a light brown oil (0.17 g, 32%). ¹H NMR (400 MHz, CDCl₃/CD₃OD): * 7.13 (d, 1H, J=8.7 Hz), 6.92 (d, 1H, J=2.3 Hz), 6.71 (dd, 1H, J=8.7 Hz, 2.4 Hz), 6.09 (s, 1H), 4.33 (t, 2H, J=7.5 Hz), 2.70 (t, 2H, J=7.5 Hz), 2.40 (s, 3H).

c). Methyl 3-(5-hydroxy-2-methylindolyl)propanoate

A solution of the product of the preceding step (0.16 g, 0.73 mmol), sodium bicarbonate (0.06 g, 0.75 mmol), and iodomethane (0.06 mL, 0.96 mmol) in N,N-dimethylformamide (10 mL) was stirred at ambient temperature for 3 days. Additional sodium bicarbonate (0.10 g, 1.25 mmol) and iodomethane (0.20 mL, 3.21 mmol) were added and the reaction stirred for another 24 h. The crude product was concentrated in vacuo, put onto a short bed of silica gel, eluted with 1:1 methylene chloride:ethyl acetate, and the eluate evaporated giving the title compound as a yellow oil (0.17 g, 97%). ¹H NMR (400 MHz, CDCl₃): * 7.12 (d, 1H, J=8.7 Hz), 6.92 (d, 1H, J=2.4 Hz), 6.70 (dd, 1H, J=8.7 Hz, 2.5 Hz), 6.12 (s, 1H), 4.53 (s, 1H), 4.35 (t, 2H, J=7.4 Hz), 3.67 (s, 3H), 2.73 (t, 2H, J=7.4 Hz), 2.41 (m, 3H).

d). Methyl 3-{2-methyl-5-[3-(2-pyridylamino)propoxy]indolyl}propanoate

A solution of the product of the preceding step (0.16 g, 0.68 mmol) and the product of Example 1, step b (0.12 g, 0.82 mmol) in anhydrous tetrahydrofuran (15 mL) was treated with tri-n-butylphosphine (0.19 mL, 0.76 mmol) and 1,1-(azodicarbonyl)dipiperidine (0.20 g, 0.79 mmol) at ambient temperature. After 18 h the reaction was concentrated in vacuo and the crude product purified by flash column:chromatography (1:1 methylene chloride:ethyl acetate eluant) giving the title compound as a pale yellow solid (94 mg, 48%). ¹H NMR (400 MHz, CDCl₃): * 8.08 (dd, 1H, J=5.0 Hz, 1.1 Hz), 7.40 (m, 1H), 7.16 (d, 1H, J=8.8 Hz), 7.00 (d, 1H, J=2.4 Hz), 6.81 (t, 1H, J=8.8 Hz, 2.4 Hz), 6.55 (m, 1H), 6.41 (d, 1H, J=8.4 Hz), 6.15 (s, 1H), 4.76 (br s, 1H), 4.36 (t, 2H, J=7.4 Hz), 4.12 (t, 2H, J=5.9 Hz), 3.67 (s, 3H), 3.52 (dd, 2H, J=12.6 Hz, 6.5 Hz), 2.73 (t, 2H, J=7.4 Hz), 2.42 (s, 3H), 2.16 (pentet, 2H, J=6.2 Hz).

e). 3-{2-Methyl-5-[3-(2-pyridylamino)propoxy]indolyl}propanoic acid sodium salt

The product of the preceding step (94 mg, 0.26 mmol) was dissolved in methanol (10 mL) and treated with 1 N aqueous sodium hydroxide (1.5 mL) at ambient temperature for 18 h. The reaction was concentrated in vacuo and the crude product purified by preparative thin-layer chromatography (10% methanol in methylene chloride as eluant) giving the title compound as pale yellow solid (34 mg, 35%). ¹H NMR (400 MHz, DMSO-d₆): * 7.95 (d, 1H, J=4.3 Hz), 7.34 (m, 1H), 7.24 (d, 1H, J=8.8 Hz), 6.92 (d, 1H, J=2.2 Hz), 6.68 (dd, 1H, J=8.6 Hz, 2.0 Hz), 6.54 (m, 1H), 6.44 (m, 2H), 6.06 (s, 1H), 4.24 (br t, 2H, J=6.9 Hz), 4.01 (t, 2H, J=6.3 Hz), 3.38 (dd, 2H, J=12.5 Hz, 6.5 Hz), 2.50 (m, 2H), 2.37 (s, 3H), 1.96 (pentet, 2H, J=6.5 Hz). Mass spectrum (LCMS, ESI pos.) Calcd. for C₂₀H₂₂N₃O₃: 354.4 (M+H). Found: 354.2.

EXAMPLE 4

2-(trans-2-{5-[3-(2-Pyridylamino)propoxy]indolyl}cyclopropyl)acetic acid

a). Ethyl 2-bromocyclopropanecarboxylate

A mixture of vinyl bromide (50 g, 0.47 mol) and rhodium (II) acetate dimer (0.1 g, 0.2 mol) was dissolved in 20 ml of 1,2-dichloroethane. Ethyl diazoacetate (20 g, 0.18 mol) was added dropwise over a period of 30 minutes. The reaction was stirred at room temperature for 4 h, the solvent was removed under vacuum, and the residue was distilled with the help of an oil pump to obtain the title compound (14 g, 16%). ¹H NMR (400 MHz, CDCl₃) * 1.27 (t, 3H, J=7.1 Hz), 1.29 (m, 1H), 1.60 (m, 1H), 2.04 (m, 1H), 3.23 (m, 1H), 4.21 (q, 2H, J=7.1 Hz).

b). Ethyl 2-(5-benzyloxyindolyl)cyclopropanecarboxylate

To a suspension of NaH (0.355 g, 14.0 mmol) in 100 ml of dry N,N-dimethylformamide was added slowly 5-benzyloxyindole (3.0 g, 13.4 mmol). When the evolution of H₂ceased, ethyl 2-bromocyclopropanecarboxylate (2.85 g, 0.0148 mol), as prepared in the preceding step, was added to the mixture and the reaction was refluxed for a period of 17 h under argon. Then the reaction was cooled down at ambient temperature and quenched carefully with water. After evaporation of the solvent under vacuum, the crude product was purified by flash chromatography on silica gel to obtain the title compound (3.45 g, 77%). ¹H NMR (400 MHz, CDCl₃) * 1.34 (t, 3H, J=7.1 Hz), 1.62 (m, 1H), 1.73 (m, 1H), 2.14 (m, 1H), 3.78 (m, 1H), 4.24 (c, 2H, J=7.1 Hz), 5.10 (s, 2H), 6.36 (dd, 1H, J=0.7, 3.2 Hz), 6.98 (dd, 1H, J=2.4, 8.8 Hz), 7.04 (d, 1H, J=3.2 Hz), 7.14 (d, 1H, J=2.4 Hz), 7.38 (m, 4H), 7.45 (m, 2H).

c). Ethyl 2-(5-hydroxyindolyl)cyclopropanecarboxylate

Ethyl 2-(5-benzyloxyindolyl)cyclopropanecarboxylate (1.75 g, 0.0052 mol), as prepared in the preceding step, was added under argon to a suspension of 10% of palladium(0) on carbon (0.50 g) in methanol (50 mL). The reaction was carried out under H₂atmosphere for a period of 6 h. Filtration of the reaction over Celite and evaporation of the filtrate yielded the title compound (1.27 g, 99%). ¹H NMR (400 MHz, CDCl₃) * 1.32 (t, 3H, J=7.1 Hz), 1.60 (m, 1H), 1.72 (m, 1H), 2.13 (m, 1H), 3.77 (m, 1H), 4.25 (q, 2H, J=7.1 Hz), 6.29 (d, 1H, J=3.0 Hz), 6.81 (d, 1H, J=8.3 Hz), 7.00 (m, 2H), 7.27 (m, 1H)

d). Ethyl 2-{5-[3-(2-pyridylamino)propoxy]indolyl}cyclopropanecarboxylate

Ethyl 2-(5-hydroxyindolyl)cyclopropanecarboxylate (0.59 g, 2.40 mmol), as prepared in the preceding step, and 3-hydropropylaminopyridine (0.37 g, 2.40 mmol), as prepared in step b of Example 1, were dissolved in tetrahydrofuran (25 mL) at ambient temperature. tri-n-Butylphosphine (0.97 g, 4.80 mL) followed by 1,1=-(azodicarbonyl)dipiperidine (1.20 g, 4.79 mmol) were added and the reaction was stirred at ambient temperature overnight. The solvent was removed under vacuum and the crude product was chromatographed on silica gel to obtain the title compound (0.38 g, 42%). ¹H-NMR (400 MHz, CDCl₃) * 1.34 (t, 3H, J=7.1 Hz), 1.69 (m, 4H), 2.12 (m, 1H), 3.51 (m, 2H), 3.78 (m, 1H), 4.12 (t, 2H, J=5.9 Hz), 4.26 (q, 2H, J=7.1 Hz), 4.80 (bs, 1H), 6.36 (dd, 1H, J=0.60, 3.1 Hz), 6.41 (d, 1H, J=8.4 Hz), 6.54 (m, 1H), 6.91 (dd, 1H, J=2.4, 8.8 Hz), 7.06 (dd, 1H, J=2.3, 9.2 Hz), 7.34 (d, 1H, J=8.8 Hz), 7.39 (m, 1H), 8.07 (m, 1H). Mass spectrum (LCMS, ESI) Calcd. for C₂₁H₂₃N₃O₃: 366.2 (M+H); Found: 366.3.

e). 2-(trans-2-{5-[3-(2-pyridylamino)propoxy]indolyl}cyclopropyl)acetic acid

Ethyl 2-{5-[3-(2-pyridylamino)propoxy]indolyl}cyclopropane-carboxylate (0.38 g, 1.056 mmol), as prepared in the preceding step, was dissolved in 7.5 mL of methanol. A solution of NaOH (0.13 g, 3.18 mmol) in water (2.5 mL) was added and the reaction was stirred at ambient temperature overnight. The base was then neutralized with an aqueous solution of HCl (3.18 mmol), and the solvent was evaporated under vacuum. The crude product was chromatographed on silica gel to obtain the title compound (200 mg, 57%). ¹H NMR (400 MHz, CDCl₃) * 1.66 (m, 2H), 2.00 (m, 1H), 2.16 (m, 2H), 3.58 (m, 2H), 3.72 (m, 1H), 4.12 (t, 2H, J=5.8 Hz), 6.31 (dd, 1H, J=0.7, 3.2 Hz), 6.77 (m, 1H,), 6.85 (dd, 1H, J=2.3, 8.8 Hz), 6.97 (d, 1H, J=9.0 Hz), 7.06 (d, 1H, J=2.3 Hz), 7.13 (d, 1H, J=3.2 Hz), 7.33 (d, 1H, J=8.8 Hz), 7.77 (m, 2H). Mass spectrum (LCMS, ESI pos.) Calcd. for C₂₁H₂₃N₃O₃352.2 (M+H);
Found: 352.2.

EXAMPLE 5

3-(5-{2-[6-(Methylamino)-2-pyridyl]ethoxy}indolyl)propanoic acid

a). (tert-Butoxy)-N-[6-methyl-(2-pyridyl)]carboxamide

A mixture of 2-amino-picoline (6.0 g, 5.5 mmol) and di-tert-butyldicarbonate (13.3 g, 6.0 mmol) was heated to 60□C overnight (16 h). The reaction was cooled and poured into saturated NH₄Cl (250 mL) and extracted ethyl acetate (2×250 mL). The combined organic layers were washed with brine, dried (Na₂SO₄), filtered and concentrated to give a yellow oil (crude 12.3 g) which was used directly in the next reaction.

b). (tert-Butoxy)-N-methyl-N-[6-methyl-(2-pyridyl)]carboxamide

To a suspension of NaH (2.63 g 6.6 mmol) in 200 mL of N,N-dimethylformamide at 0□C was added a solution of (tert-butoxy)-N-[6-methyl-(2-pyridyl)]carboxamide (12.3 g, crude), as prepared in the preceding step, in 50 mL of N,N-dimethylformamide. The reaction stirred at 0□C for 15 min then at ambient temperature for 1 h. Then iodomethane (10.22 g, 7.2 mmol) was added and the mixture was stirred at ambient temperature overnight (16 h). The reaction mixture was concentrated in vacuo, diluted with saturated NH₄Cl (400 mL), and extracted with ethyl acetate (2×250 mL). The combined organic layers were washed with brine, dried (Na₂SO₄), filtered and concentrated. The residue was purified by flash chromatography on silica gel (10% ethyl acetate in hexane) to give the title compound as a yellow oil (7.56 g, 57%). ¹H-NMR (400 MHz, DMSO-d₆) * 7.63 (t, J=7.2 Hz, 1H), 7.37 (d, J=8.0 Hz, 1H), 6.97 (d, J=6.9 Hz, 1H), 3.27 (s, 2H), 2.42 (s, 3H), 1.45 (s, 9H).

c). Ethyl 2-{6-[(tert-butoxy)-N-methylcarbonylamino]-2-pyridyl}acetate

Lithium diisopropylamide (6.6 mmol) was prepared in tetrahydrofuran (60 mL), cooled to −78□C, and (tert-butoxy)-N-methyl-N-[6-methyl-(2-pyridyl)]carboxamide (7.56 g, 3.3 mmol), as prepared in the preceding step, was dissolved in tetrahydrofuran (100 mL) and added dropwise over 30 min. The mixture was stirred for 15 min then diethylcarbonate (6.24 g, 5.3 mmol) was added. The solution was stirred for an additional 15 min, then allowed to warm to 0□C over 2 h. The reaction was quenched with saturated NH₄Cl solution (200 mL). The mixture was allowed to warm to ambient temperature and extracted with ethyl acetate (2×100 mL). The combined organic layers were washed with brine, dried (Na₂SO₄), filtered and concentrated. The residue was purified by flash chromatography (silica gel, 10% ethyl acetate in hexane) to give the title compound as a yellow oil (5.51 g, 60%). ¹H-NMR (400 M Hz, DMSO-d₆) δ 7.71 (t, J=7.9 Hz, 1H), 7.49 (d, J=8.2 Hz, 1H), 7.07 (d, J=7.4 Hz, 1H), 4.09 (q, J=7.1 Hz, 2H), 3.78 (s, 2H), 2.54 (s, 3H), 1.46 (s, 9H), 1.18 (t, J=7.1 Hz, 3H).

d). Ethyl 2-[6-(methylamino)-2-pyridylacetate

A solution of ethyl 2-{6-[(tert-butoxy)-N-methylcarbonylamino]-2-pyridyl}acetate (5.51 g, 1.9 mmol), as prepared in the preceding step, in methylene chloride (25 mL) was stirred in an ice bath at 0□C. Trifluoroacetic acid (10 mL) was then added and the solution were allowed to warm to ambient temperature and stirred overnight (16 h). The reaction mixture was concentrated, 10% aqueous K₂CO₃(300 mL) was added and the mixture was extracted with ethyl acetate (2×100 mL). The combined organic layers were washed with brine, dried (Na₂SO₄), filtered and concentrated to give the title compound as a bright yellow oil (3.4 g, 100%). ¹H-NMR (400 MHz, DMSO-d₆) δ 7.32 (t, J=7.2 Hz, 1H), 6.40 (d, J=7.0 Hz, 1H), 6.29 (d, J=8.3 Hz, 1H), 4.07 (q, J=7.1 Hz, 2H), 3.56 (s, 2H), 2.71 (d, J=4.9 Hz, 3H), 1.17 (t, J=7.1 Hz, 3H).

e). 2-[6-(Methylamino)-2-pyridyl]ethan-1-ol

To a suspension of lithium aluminum hydride (1.8 g, 4.9 mmol) in tetrahydrofuran (50 mL) was added dropwise a solution of ethyl 2-[6-(methylamino)-2-pyridylacetate (3.5 g, 1.9 mmol), as prepared in the preceding step, in tetrahydrofuran (50 mL) at 0□C. After the addition was completed, the reaction mixture was stirred at 0□C for 30 minutes then stirred at ambient temperature for 2 h. The reaction mixture was then cooled back to 0□C and quenched with H₂O (1.8 mL), 10% NaOH (1.8 mL) and H₂O (3.0 mL) and allowed to warm back to ambient temperature. The solids were removed by filtration through Celite and washed with tetrahydrofuran (100 mL). The filtrate was dried (Na₂SO₄), filtered and concentrated. The residue was purified by flash chromatography on silica gel (3% methanol in methylene chloride) to give the title compound as a yellow oil (2.1 g, 70%). ¹H-NMR (400 MHz, CDCl₃) δ 7.36 (t, J=7.8 Hz, 1H), 6.41 (d, J=7.2 Hz, 1H), 6.26 (d, J=8.3 Hz, 1H), 4.51 (br s, 1H), 3.96 (t, J=5.2 Hz, 2H), 2.89 (d, J=5.1 Hz, 3H), 2.84 (t, J=5.4 Hz, 2H).

f). Methyl 3-(5-benzyloxyindolyl)propanoate

To a solution of 5-benzyloxyindole (1.15 g, 5 mmol) in N,N-dimethylformamide (40 mL) was added sodium hydride (200 mg, 5 mmol). After stirring for 30 minutes, ethyl bromopropionate (900 mg, 5.0 mmol) was added and the mixture was stirred at ambient temperature for 1 h, additional sodium hydride (100 mg, 2.5 mmol) was added. After stirring for 10 minutes, additional ethyl bromopropionate (180 mg, 1.0 mmol) was added. The mixture was stirred at ambient temperature overnight. The solvent was removed under high vacuum, the residue was dissolved in water (10 mL) and tetrahydrofuran (10 mL), NaOH (500 mg) was added and stirred for 2 h. After acidifying to pH 4-5, the mixture was extracted with methylene chloride. The methylene chloride layer washed with brine and dried over Na₂SO₄. After evaporating the solvent in vacuo, the residue was purified by flash column chromatography (1-5% ethyl acetate in methylene chloride) to give 3-(5-benzyloxyindolyl)propanic acid as white solid. The solid was dissolved in N,N-dimethylformamide (20 mL), K₂CO₃(110 g) and iodomethane (840 mg) were added and the reaction was stirred at ambient temperature for 3 h. The mixture was concentrated under high vacuum and residue was purified by flash column chromatography (methylene chloride) to give the title compound as a colorless oil (1.10 g, 71%). ¹H-NMR (400 MHz, CDCl₃) δ 7.47 (d, J=7.3 Hz, 2H), 7.38 (t, J=7.3 Hz, 2H), 7.32 (d, J=7.2 Hz, 1H), 7.23 (d, J=10.2 Hz, 1H), 7.15 (d, J=2.3 Hz, 1H), 7.09 (d, J=3.1 Hz, 1H), 6.96 (dd, J=8.8, 2.5 Hz, 1H), 6.39 (d, J=3.1 Hz, 1H), 5.10 (s, 2H), 4.41 (t, J=6.9 Hz, 2H), 3.66 (s, 3H), 2.81 (t, J=6.8 Hz, 2H).

g). Methyl 3-(5-hydroxyindolyl)propanoate

A mixture of methyl 3-(5-benzyloxyindolyl)propanoate (1.1 g, 3.56 mmol), as prepared in the preceding step, 10% palladium(0) on carbon (100 mg) in ethanol was stirred under hydrogen for 3 h. The catalyst was removed by filtration, the filtrate was concentrated in vacuo and the residue was purified by flash column chromatography (1-5% ethyl acetate in methylene chloride) to give the title compound as a pale yellow oil (700 mg, 90%). ¹H-NMR (400 M Hz, CDCl₃) δ 7.18 (d, J=8.8 Hz, 1H), 7.08 (d, J=3.1 Hz, 1H), 7.02 (d, J=3.2 Hz, 1H), 6.77 (dd, J=8.8, 2.5 Hz, 1H), 6.34 (d, J=3.1 Hz, 1H), 4.75 (s, 1H), 4.40 (t, J=6.9 Hz, 2H), 3.66 (s, 3H), 2.81 (t, J=6.9 Hz, 2H).

h). Methyl 3-(5-{2-[6-(methylamino)-2-pyridyl]ethoxy}indolyl)propanoate

Diisopropyl azodicarboxylate (0.19 g, 0.94 mmol) was added to a solution of 2-[6-(methylamino)-2-pyridyl]ethan-1-ol (0.10 g, 0.66 mmol), as prepared in step e of Example 5, methyl 3-(5-hydroxyindolyl)propanoate (0.10 g, 0.46 mmol), as prepared in the preceding step, and triphenylphosphine (0.24 g, 0.92 mmol) in tetrahydrofuran (5.0 mL) at 0□C in an ice bath. After stirring at ambient temperature overnight (16 h), the reaction was concentrated and the residue was purified by flash chromatography on silica gel (20%-30% ethyl acetate in hexane) to give the title compound as a yellow oil (0.023 g, 15%). ¹H-NMR (400 M Hz, CDCl₃) δ 7.39 (t, J=7.3 Hz, 1H), 7.20 (d, J=8.9 Hz, 1H), 7.11 (d, J=2.3 Hz, 1H), 7.07 (d, J=3.1 Hz, 1H), 6.87 (dd, J=2.4, 8.9 Hz, 1H), 6.56 (d, J=7.2 Hz, 1H), 6.37 (d, J=3.1 Hz, 1H), 6.24 (d, J=8.2 Hz, 1H), 4.56 (br s, 1H), 3.40 (t, J=6.9 Hz, 2H), 4.34 (t, J=7.0 Hz, 2H), 3.65 (s, 3H), 3.10 (t, J=7.0 Hz, 2H), 2.89 (d, J=4.8 Hz, 2H), 2.80 (t, J=6.9 Hz, 2H).

i). 3-(5-{2-[6-(methylamino)-2-pyridyl]ethoxy}indolyl)propanoic acid

To a solution of methyl 3-(5-{2-[6-(methylamino)-2-pyridyl]ethoxy}indolyl)propanoate (0.023 g, 0.65 mmol), as prepared in the preceding step, in methanol (3 mL) was added sodium hydroxide (0.15 g, 3.8 mmol) in H₂O (0.5 mL) and the reaction was stirred for 6 hours at ambient temperature. After evaporating the solvent in vacuo, the residue is taken up in H₂O (5 mL) and acidified to pH 4-5 with 10% HCl, extracted with a mixture of ethyl acetate and butanol (2×50 mL) and the combined organic layers were washed with brine, dried (Na₂SO₄), filtered and concentrated to give the title compound as a solid (0.018 g, 82%). ¹H-NMR (400 M Hz, CDCl₃+CD₃OD) δ 7.52 (t, J=7.3 Hz, 1H), 7.25 (d, J=8.9 Hz, 1H), 7.14 (d, J=3.1 Hz, 1H), 7.06 (d, J=2.3 Hz, 1H), 6.81 (dd, J=8.9, 2.4 Hz, 1H), 6.60 (d, J=7.3 Hz, 1H), 6.38 (d, J=8.6 Hz, 1H), 6.33 (d, J=3.2 Hz, 1H), 4.38 (t, J=7.0 Hz, 2H), 4.24 (t, J=6.6 Hz, 2H), 3.06 (t, J=6.6 Hz, 2H), 2.89 (s, 3H), 2.77 (t, J=6.9 Hz, 2H). Mass spectrum (LCMS, ESI pos.) Calcd. for C₁₉H₂₁N₃O₃340.3 (M+H); Found: 340.9.

EXAMPLE 6

2-Benzyl-3-{5 [3-(2-pyridylamino)propoxy]indolyl}propanoic acid

a). Methyl 3-[5-(benzyloxyindolyl]-2-benzylpropanoate

Lithium diisopropylamide (0.55 mmol) was prepared in tetrahydrofuran (4.0 mL), cooled to −78□C, and treated with a solution of methyl 3-(5-benzyloxyindolyl)propanoate (0.15 g, 0.49 mmol), as prepared in the step f of Example 5, in tetrahydrofuran (4.0 mL). After stirring for 90 min at −78_C, benzyl bromide (0.08 g, 0.49 mmol) was added and the reaction mixture was allowed to warm to ambient temperature slowly over 3 h. The reaction mixture was poured into saturated NH₄Cl (20 mL) and extracted with ethyl acetate (2×50 mL). The combined organic layers were washed with brine, dried (Na₂SO₄), filtered and concentrated. The residue was purified by flash chromatography on silica gel (8% ethyl acetate in hexane) to give the title compound as an oil (0.09 g, 50%). ¹H-NMR (400 MHz, CDCl₃) δ 7.46 (d, J=7.2 Hz, 2H), 7.30 (m, 4H), 7.26 (m, 1H), 7.16 (m, 3H), 7.00 (m, 2H), 6.88 (dd, J=2.4, 8.6 Hz, 1H), 6.36 (m, 1H), 5.08 (s, 2H), 4.40 (dd, J=8.9, 13.9 Hz, 1H), 4.15 (dd, J=14.4, 5.3 Hz, 1H), 3.50 (s, 3H), 3.23, (m, 1H), 3.04 (dd, J=13.1, 7.8 Hz, 1H), 2.76 (dd, J=14.4, 7.1 Hz, 1H).

b). Methyl 3-(5-hydroxylindolyl)-2-benzylpropanoate

A mixture of methyl 3-(5-benzyloxyindolyl)-2-benzylpropanoate (0.16, 0.39 mmol), as prepared in the preceding step, 10% palladium(0) on carbon (0.02 g) in ethanol (10 mL) was stirred at ambient temperature under hydrogen (balloon) overnight (16 h). The catalyst was removed by filtration through Celite. The filtrate was concentrated to give the title compound as a light brown oil (0.12 g, 100%) which was used directly in next reaction.

c). Methyl 2-benzyl-3-{5-[2-(pyridylamino)propoxy]indolyl}propanoate

1,1′-(Azodicarbonyl)dipiperidine (0.18 g, 0.7 mmol) was added to a solution of methyl 3-(5-hydroxylindolyl)-2-benzylpropanoate (0.12 g, 0.39 mmol), as prepared in the preceding step, 2-(3-hydroxypropyl)aminopyridine (0.07 g, 0.47 mmol), as prepared in step b of Example 1, and tri-n-butylphosphine (0.14 g, 0.7 mmol) in tetrahydrofuran (6.0 mL). After stirring at ambient temperature overnight (16 h), the reaction was concentrated in vacuo and the residue purified by flash chromatography on silica gel (10%-50% ethyl acetate in hexane) to give the title compound as a yellow oil (0.064 g, 38%). ¹H-NMR (400 MHz, CDCl₃) δ 8.08 (br s, 1H), 7.40 (m, 1H), 7.37 (m, 1H), 7.22 (m, 2H), 7.00 (m, 3H), 6.84 (dd, J=8.9, 2.4 Hz, 1H), 6.54 (m, 1H), 6.40 (d, J=8.4 Hz, 2H), 6.36 (d, J=3.1 Hz, 1H), 4.77 (br s, 1H), 4.40 (m, 1H), 4.17 (m, 3H), 3.52 (m, 5H), 3.24 (m, 1H), 3.08 (m, 1H), 2.76 (m, 1H), 2.11 (m, 2H).

d). 2-Benzyl-3-{5 [3-(2-pyridylamino)propoxy]indolyl}propanoic acid

To a solution of methyl 2-benzyl-3-{5-[2-(pyridylamino)propoxy]indolyl}propanoate (0.06 g, 0.13 mmol), as prepared in the preceding step, in methanol (3.0 mL) was added a solution of NaOH (0.1 g, 2.5 mmol) in H₂O (0.3 mL), and the reaction was stirred at ambient temperature overnight. After evaporating the solvent in vacuo, the residue is mixed with H₂O (5 mL) and acidified to pH 4-5 with 10% HCl, extracted with ethyl acetate (2×25 mL). The combined organic layers were washed with brine, dried (Na₂SO₄), filtered and concentrated. The residue was purified by flash chromatography on silica gel (4% methanol in methylene chloride) to give the title compound as an oil (0.043 g, 80%). ¹H-NMR (400 MHz, CDCl₃) δ 7.66 (d, J=4.7 Hz, 1H), 7.50 (m, 1H), 7.22 (m, 3H), 7.11 (d, J=8.9 Hz, 1H), 7.04 (d, J=2.8 Hz, 1H), 6.95 (d, J=2.3 Hz, 1H), 6.71 (dd, J=8.8, 2.3 Hz, 1H), 6.50 (m, 2H), 6.24 (d, J=2.7 Hz, 1H), 4.32 (m, 1H), 4.0 (m, 1H), 3.91 (t, J=5.7 Hz, 2H), 3.28 (t, J=6.6 Hz, 2H), 3.15 (m, 1H), 2.75 (m, 1H), 1.93 (m, 2H). Mass spectrum (LCMS, ESI pos.) Calcd. for C₂₆H₂₇N₃O₃430.5 (M+H); Found: 430.2.

EXAMPLE 7

2-Methyl-3-{5-[3-(2-pyridylamino)propoxy]indolyl}propanoic acid

a). Methyl 2-methyl-3-(5-benzyloxyindolyl)propanoate

Lithium diisopropylamide (0.99 mmol) was prepared in tetrahydrofuran (4.0 mL), cooled to −78□C, and methyl 3-(5-benzyloxyindolyl)propanoate (0.19 g, 0.62 mmol), as prepared in step f of Example 5, was added dropwise in tetrahydrofuran (4.0 mL). After stirring for 90 min at −78□C, iodomethane (0.44 g, 3.1 mmol) was added and the reaction was allowed to warm to ambient temperature slowly over 3 h. The reaction mixture was poured into saturated NH₄Cl (20 mL) and extracted with ethyl acetate (2×50 mL). The combined organic layers were washed with brine, dried (Na₂SO₄), filtered and concentrated. The residue was purified by flash chromatography on silica gel (8% ethyl acetate in hexane) to give the title compound as an oil (0.18 g, 90%). ¹H-NMR (400 MHz, CDCl₃) δ 7.47 (d, J=6.9 Hz, 2H), 7.39 (m, 2H), 7.33 (m, 1H), 7.24 (d, J=8.9 Hz, 1H), 7.15 (d, J=2.4 Hz, 1H), 7.04 (d, J=3.1 Hz, 1H), 6.95 (dd, J=8.9, 2.5 Hz, 1H), 6.38 (d, J=3.2 Hz, 1H), 5.10 (s, 2H), 4.42 (dd, J=14.4, 7.3 Hz, 1H), 4.08 (dd, J=14.4, 7.1 Hz, 1H), 3.63 (s, 3H), 3.01 (q, J=7.1 Hz, 1H), 1.16 (d, J=7.1 Hz, 3H).

b). Methyl 3-(5-hydroxyindolyl)-2-methylpropanoate

A mixture of methyl 2-methyl-3-[5-benzyloxyindolyl]propanoate (0.18 g, 0.56 mmol), as prepared in the preceding step, 10% palladium(0) on carbon (0.018 g) in ethanol (10 mL) was stirred at ambient temperature under hydrogen (balloon) overnight (16 h). The catalyst was removed by filtration through Celite. The filtrate was concentrated to give the title compound as a light brown oil (0.11 g, 85%) which was used directly in the next reaction.

c). Methyl 2-methyl-3-{5-[3-(2-pyridylamino)propoxy]indolyl}propanoate

1,1′-(Azodicarbonyl)dipiperidine (0.13 g, 0.57 mmol) was added to the solution of methyl 3-(5-hydroxyindolyl)-2-methylpropanoate (0.062 g, 0.27 mmol), as prepared in the preceding step, 2-(3-hydroxypropyl)aminopyridine (0.06 g, 0.40 mmol), as prepared in step b of Example 1, and tri-n-butylphosphine (0.11 g, 0.53 mmol) in tetrahydrofuran (6.0 mL). After stirring at ambient temperature overnight (16 h), the reaction was concentrated and the residue was purified by flash chromatography on silica gel (10%-50% ethyl acetate in hexane) to give the title compound as a yellow oil (0.015 g, 15%). ¹H-NMR (400 MHz, CDCl₃) δ 8.07 (m, 1H), 7.40 (m, 1H), 7.21 (d, J=8.9 Hz, 1H), 7.08 (d, J=2.4 Hz, 1H), 7.04 (d, J=3.1 Hz, 1H), 6.88 (dd, J=8.9, 2.4 Hz, 1H), 6.54 (m, 1H), 6.41 (m, 1H), 4.89 (br s, 1H), 4.45 (dd, J=14.2, 7.3 Hz, 1H), 4.10 (m, 4H), 3.63 (s, 3H), 5.52 (q, J=6.5 Hz, 2H), 2.13 (m, 2H), 1.66 (m, 1H), 1.52 (m, 1H), 1.16 (d, J=7.1 Hz, 3H), 0.93 (m, 3H).

d). 2-methyl-3-{5-[3-(2-pyridylamino)propoxy]indolyl}propanoic acid

To a solution of methyl 2-methyl-3-{5-[3-(2-pyridylamino)propoxy]indolyl}propanoate (0.015 g, 0.04 mmol), as prepared in the preceding step, in methanol (5.0 mL) was added a solution of NaOH (0.1 g, 2.5 mmol) in H₂O (0.3 mL), and the reaction was stirred at ambient temperature overnight. After evaporating the solvent in vacuo, the residue is taken up in H₂O (5 mL) and acidified to pH 4-5 with 10% HCl and extracted with ethyl acetate (2×15 mL). The combined organic layers were washed with brine, dried (Na₂SO₄), filtered and concentrated. The residue was purified by flash chromatography on silica gel (4% methanol in methylene chloride) to give the title compound as an oil (0.011 g, 80%). ¹H-NMR (400 MHz, CDCl₃) δ 7.86 (d, J=5.6 Hz, 1H), 7.50 (m, 1H), 7.26 (d, J=8.9 Hz, 1H), 7.07 (dd, J=13.1, 2.8 Hz, 2H), 6.84 (dd, J=8.9, 2.4 Hz, 1H), 6.56 (m, 2H), 6.32 (d, J=2.0 Hz, 1H), 4.38 (dd, J=14.3, 7.0 Hz, 1H), 4.07 (t, J=5.8 Hz, 2H), 4.01 (dd, J=14.3, 7.5 Hz, 1H), 3.44 (t, J=6.7 Hz, 2H), 2.92 (q, J=7.1 Hz, 1H), 2.08 (m, 2H), 1.12 (d, J=7.1 Hz, 3H). Mass spectrum (LCMS, ESI pos.) Calcd. for C₂₀H₂₃N₃O₃354.3 (M+H); Found: 354.2.

EXAMPLE 8

2-({5-[3-(2-Pyridylamino)propoxy]indolyl}methyl)pentanoic acid

a). Methyl 2-[(5-benzyloxyindolyl)methyl]pentanoate

Lithium diisopropylamide (0.51 mmol) was prepared in tetrahydrofuran (4.0 mL), cooled to −78□C, and methyl 3-(5-benzyloxyindolyl)propanoate (0.14 g, 0.46 mmol), as prepared in step f of Example 5, was added dropwise in tetrahydrofuran (4.0 mL). After stirring for 90 min at −78□C, iodopropane (0.08 g, 0.46 mmol) was added and the reaction mixture was allowed to warm to ambient temperature slowly over 3 h. The reaction mixture was poured into saturated NH₄Cl (20 mL) and extracted with ethyl acetate (2×50 mL). The combined organic layers were washed with brine, dried (Na₂SO₄), filtered and concentrated. The residue was purified by flash chromatography on silica gel (8% ethyl acetate in hexane) to give the title compound as an oil (0.025 g, 16%). ¹H-NMR (400 MHz, CDCl₃) δ 7.47 (m, 2H), 7.39 (m, 2H), 7.31 (m, 1H), 7.21 (d, J=8.9 Hz, 1H), 7.15 (d, J=2.4 Hz, 1H), 7.02 (d, J=3.1 Hz, 1H), 6.95 (dd, J=8.9, 2.4 Hz, 1H), 6.37 (dd, J=3.1, 0.7 Hz, 1H), 5.10 (s, 2H), 4.37 (dd, J=14.4, 8.5 Hz, 1H), 4.15 (dd, J=14.4, 6.1 Hz, 1H), 3.57 (s, 3H), 2.95 (m, 1H), 1.64 (m, 1H), 1.42 (m, 3H), 0.90 (t, J=7.3 Hz, 3H).

b). Methyl 2-[(5-hydroxyoxyindolyl)methylpentanoate

A mixture of methyl 2-[(5-benzyloxyindolyl)methyl]pentanoate (0.036 g), as prepared in the preceding step, 10% palladium(0) on carbon (0.005 g) in ethanol (5 mL) was stirred at ambient temperature under hydrogen (balloon) overnight (16 h). The catalyst was removed by filtration through Celite. The filtrate was concentrated to give the title compound as a light brown oil (0.03 g, 100%) which was used directly in the next reaction.

c). Methyl 2-({5-[3-(2-pyridylamino)propoxy]indolyl}methyl)pentanoate

1,1′-(Azodicarbonyl)dipiperidine (0.12 g, 0.48 mmol) was added to the solution of methyl 2-[(5-hydroxyindolyl)methyl]pentanoate (0.03 g, 0.12 mmol), as prepared in the preceding step, 2-(3-hydroxypropyl)aminopyridine (0.026 g, 0.17 mmol), as prepared in step b of Example 1, and tri-n-butylphosphine (0.09 g, 0.46 mmol) in tetrahydrofuran (6.0 mL). After stirring at ambient temperature overnight (16 h), the reaction was concentrated and the residue was purified by flash chromatography on silica gel (10%-50% ethyl acetate in hexane) to give the title compound as a yellow oil (0.016 g, 36%). ¹H-NMR (400 MHz, CDCl₃) δ 8.08 (m, 1H), 7.39 (m, 1H), 7.20 (d, J=8.9 Hz, 1H), 7.07 (d, J=2.4 Hz, 1H), 7.02 (d, J=3.3 Hz, 1H), 6.88 (dd, J=8.9, 2.4 Hz, 1H), 6.53 (m, 1H), 6.40 (m, 2H), 4.32 (br s, 1H), 4.42 (m, 1H), 4.25 (m, 3H), 3.52 (m, 5H), 2.91 (m, 1H), 2.20 (m, 2H), 1.72 (m, 2H), 1.43 (m, 3H), 0.95 (t, J=7.2 Hz, 3H).

d). 2-({5-[3-(2-Pyridylamino)propoxy]indolyl}methyl)pentanoic acid

To a solution of methyl 2-({5-[3-(2-pyridylamino)propoxy]indolyl}methyl)pentanoate (0.015 g, 0.004 mmol), as prepared in the preceding step, in methanol (2.0 mL) was added a solution of NaOH (0.1 g, 2.5 mmol) in H₂O (0.3 mL), and the reaction was stirred at ambient temperature overnight. After evaporating the solvent in vacuo, the residue is taken up in H₂O (5 mL) and acidified to pH 4-5 with 10% HCl, and extracted with ethyl acetate (2×15 mL). The combined organic layers were washed with brine, dried (Na₂SO₄), filtered and concentrated. The residue was purified by flash chromatography on silica gel (4% methanol in methylene chloride) to give the title compound as an oil (0.011 g, 85%). ¹H-NMR (400 MHz, CDCl₃) δ 8.28 (br s, 1H), 7.72 (d, J=4.5 Hz, 1H), 7.49 (m, 1H), 7.26 (d, J=8.9 Hz, 1H), 7.06 (d, J=3.0 Hz, 1H), 6.99 (d, J=2.3 Hz, 1H), 6.78 (dd, J=8.9, 2.4 Hz, 1H), 6.5 (m, 2H), 6.27 (d, J=2.8 Hz, 1H), 4.25 (dd, J=14.1, 8.4 Hz, 1H), 3.97 (m, 3H), 3.33 (t, J=6.6 Hz, 2H), 2.87 (br s, 1H), 1.96 (m, 2H), 1.67 (m, 1H), 1.45 (m, 3H), 0.90 (t, J=6.8 Hz, 3H). Mass spectrum (LCMS, ESI pos.) Calcd. for C₂₂H₂₇N₃O₃382.5 (M+H); Found: 382.2.

EXAMPLE 9

2-({5-[3-(2-Pyridylamino)propoxy]indolyl}methyl)octanoic acid

a) Methyl 2-[(5-benzylindolyl)methyloctanoate

Lithium diisopropylamide (1.3 mmol) was prepared in tetrahydrofuran (4.0 mL), cooled to −78□C, and a solution of methyl 3-(5-benzyloxyindolyl)propanoate (0.21 g, 0.7 mmol), as prepared in step f of Example 5, was added dropwise in tetrahydrofuran (4.0 mL). After stirring for 90 min at −78□C, iodohexane (0.7 g, 3.4 mmol) was added and the reaction mixture was allowed to warm to ambient temperature over 3 h. The reaction mixture was poured into saturated NH₄Cl (20 mL) and extracted with ethyl acetate (2×50 mL). The combined organic layers were washed with brine, dried (Na₂SO₄), filtered and concentrated. The residue was purified by flash chromatography on silica gel (8% ethyl acetate in hexane) to give the title compound as an oil (0.13 g, 50%). ¹H-NMR (400 MHz, CDCl₃) _—7.47 (d, J=7.0 Hz, 1H), 7.37 (m, 2H), 7.32 (m, 1H), 7.20 (d, J=8.9 Hz, 1H), 7.14 (d, J=2.4 Hz, 1H), 7.02 (d, J=3.1 Hz, 1H), 6.95 (dd, J=8.9, 2.5 Hz, 1H), 6.37 (d, J=3.1 Hz, 1H), 5.09 (s, 2H), 4.35 (dd, J=14.4, 8.5 Hz, 1H), 4.13 (dd, J=14.4, 6.1 Hz, 1H), 3.57 (s, 3H), 2.91 (m, 1H), 1.64 (m, 1H), 1.48 (m, 1H), 1.30 (m, 6H), 0.87 (t, J=6.9 Hz, 3H).

b) Methyl 2-[(5-hydroxyindolyl)methyloctanoate

A mixture of methyl 2-[(5-benzyloxyindolyl)methyloctanoate (0.15 g, 0.37 mmol), as prepared in the preceding step, 10% palladium(0) on carbon in ethanol (10 mL) was stirred at ambient temperature under hydrogen (balloon) overnight (16 h). The catalyst was removed by filtration through Celite. The filtrate was concentrated to give the title compound as a light brown oil (0.11 g, 100%) which was used directly in the next reaction.

c) Methyl 2-({5-[3-(2-pyridylamino)propoxy]indolyl}methyl)octanoate

1,1′-(Azodicarbonyl)dipiperidine (0.19 g, 0.75 mmol) was added to the solution of methyl 2-[(5-hydroxyindolyl)methyloctanoate (0.11 g, 0.38 mmol), as prepared in the preceding step, 2-(3-hydroxypropyl)aminopyridine (0.09 g, 0.56 mmol), as prepared in step b of Example 1, and tri-n-butylphosphine (0.13 g, 0.75 mmol) in tetrahydrofuran (6.0 mL). After stirring at ambient temperature overnight (16 h), the reaction was concentrated and the residue was purified by flash chromatography on silica gel (10%-50% ethyl acetate in hexane) to give the title compound as a yellow oil (0.04 g, 25%). ¹H-NMR (400 MHz, CDCl₃) δ 8.80 (m, 1H), 7.40 (m, 1H), 7.21 (d, J=9.0 Hz, 1H), 7.08 (d, J=2.3 Hz, 1H), 7.03 (d, J=3.1 Hz, 1H), 6.88 (dd, J=9.2, 2.4 Hz, 1H), 6.55 (m, 2H), 6.40 (m, 2H), 4.81 (br s, 1H), 4.37 (dd, J=14.4, 8.5 Hz, 1H), 4.14 (m, 3H), 3.69 (s, 3H), 3.65 (m, 2H), 2.94 (m, 1H), 2.15 (m, 2H), 1.50 (m, 7H), 0.90 (m, 3H).

d) 2-({5-[3-(2-pyridylamino)propoxy]indolyl}methyl)octanoic acid

To a solution of methyl 2-({5-[3-(2-pyridylamino)propoxy]-indolyl}methyl)octanoate (0.04 g, 0.09 mmol), as prepared in the preceding step, in methanol (5.0 mL) was added a solution of NaOH (0.1 g, 2.5 mmol) in H₂O (0.3 mL), and the reaction was stirred at ambient temperature overnight. After evaporating the solvent in vacuo, the residue is taken up in H₂O (5 mL) and acidified to pH 4-5 with 10% HCl, and extracted with ethyl acetate (2×15 mL). The combined organic layers were washed with brine, dried (Na₂SO₄), filtered and concentrated. The residue was purified by flash chromatography on silica gel (4% methanol in methylene chloride) to give the title compound as an oil (0.34 g, 90%). ¹H-NMR (400 MHz, CDCl₃) δ 7.72 (m, 1H), 7.48 (m, 1H), 7.26 (d, 8.9 Hz, 1H), 7.02 (d, J=2.7 Hz, 2H), 6.77 (dd, J=8.9, 2.4 Hz, 1H), 6.50 (m, 2H), 6.28 (d, J=2.9 Hz, 1H), 4.27 (dd, J=14.2, 8.6 Hz, 1H), 3.99 (m, 3H), 3.32 (t, J=6.7 Hz, 2H), 2.85 (br s, 1H), 1.95 (m, 2H), 1.67 (m, 1H), 1.30 (m, 9H), 0.84 (t, J=6.6 Hz, 3H). Mass spectrum (LCMS, ESI pos.) Calcd. for C₂₅H₃₃N₃O₃424.2 (M+H); Found: 424.7.

EXAMPLE 10

3-[5-(3-{[Benzylamino]carbonylamino}propoxy)indolyl]propanoic acid

a) Methyl 3-{5-[3-(benzyloxycarbonylamino)propoxy]indolyl}propanoate

1,1=-(Azodicarbonyl)dipiperidine (370 mg, 1.5 mmol) was added to the solution of methyl 3-(5-hydroxyindolyl)propanoate (220 mg, 1.0 mmol), as prepared in step g of Example 5,3-(benzyloxycarbonylamino)propanol (230 mg, 1.1 mmol) and tri-n-butylphosphine (305 mg, 1.5 mmol) in tetrahydrofuran (20 mL). After stirring at ambient temperature overnight, the reaction mixture was concentrated and the residue was purified by flash column chromatography on silica gel (0-2% ethyl acetate in methylene chloride) to give the title compound as an off white solid (310 mg, 76%). ¹H-NMR (400 MHz, CDCl₃) δ 7.35 (m, 5H), 7.22 (d, J=8.9 Hz, 1H), 7.09 (d, J=3.1 Hz, 1H), 7.07 (d, J=2.1 Hz, 1H), 6.86 (dd, J=8.8, 2.4 Hz, 1H), 6.38 (d, J=2.9 Hz, 1H), 5.11 (br s, 3H), 4.41 (t, J=6.8 Hz, 2H), 4.07 (t, J=5.9 Hz, 2H), 3.66 (s, 3H), 3.44 (q, J=6.3 Hz, 2H), 2.81 (t, J=6.8 Hz, 2H), 2.02 (m, 2H).

b) Methyl 3-[5-(aminopropoxy)indolyl]propanoate

A mixture of methyl 3-{5-[3-(benzyloxycarbonylamino)propoxy]-indolyl}propanoate (300 mg, 0.73 mmol), as prepared in the preceding step, 10% palladium(0) on carbon (50 mg) in ethanol (20 mL) was stirred at ambient temperature under hydrogen (balloon) for 3 h. The catalyst was removed by filtration through Celite. The filtrate was concentrated to give the title compound as an off white solid (150 mg, 74%). ¹H-NMR (400 MHz, CDCl₃/CD₃OD) δ 7.25 (d, J=8.9 Hz, 1H), 7.13 (d, J=2.6 Hz, 1H), 7.08 (d, J=2.5 Hz, 1H), 6.85 (dd, J=8.9, 2.5 Hz, 1H), 6.38 (d, J=2.9 Hz, 1H), 4.43 (t, J=6.8 Hz, 2H), 4.10 (t, J=5.8 Hz, 2H), 3.66 (s, 3H), 3.01 (q, J=7.0 Hz, 2H), 2.83 (t, J=6.8 Hz, 2H), 2.04 (m, 2H).

c) Methyl 3-[5-(3-{[benzylamino]carbonylamino}propoxy)indolyl]propanoate

To the solution of methyl 3-[5-(aminopropoxy)indolyl]propanoate (140 mg, 0.5 mmol), as prepared in the preceding step, in acetonitrile (10 mL) was added benzyl isocyanate (135 mg, 1.0 mmol), and the mixture was stirred at ambient temperature overnight. After evaporating the solvent in vacuo, the residue was purified by flash column chromatography on silica gel (methylene chloride to 5% ethyl acetate in methylene chloride) to give the title compound as a white solid (85 mg, 42%). ¹H-NMR (400 MHz, CDCl₃) δ 7.28 (m, 5H), 7.20 (d, J=8.9 Hz, 1H), 7.10 (d, J=2.8 Hz, 1H), 7.05 (d, J=2.5 Hz, 1H), 6.81 (dd, J=8.8, 2.5 Hz, 1H), 6.38 (d, J=2.9 Hz, 1H), 4.66 (br s, 2H), 4.41 (t, J=6.8 Hz, 2H), 4.35 (d, J=5.7 Hz, 2H), 4.06 (t, J=5.8 Hz, 2H), 3.66 (s, 3H), 3.43 (q, J=6.2 Hz, 2H), 2.81 (t, J=6.8 Hz, 2H), 1.99 (t, J=6.1, 2H).

d) 3-[5-(3-{[Benzylamino]carbonylamino}propoxy)indolyl]propanoic acid

To the solution of methyl 3-[5-(3-{[benzylamino]carbonylamino}propoxy)indolyl]propanoate (80 mg, 0.2 mmol), as prepared in the preceding step, in tetrahydrofuran (5 mL) and water (5 mL) was added sodium hydroxide (20 mg), and the reaction mixture was stirred at ambient temperature for 2 h. After evaporating the tetrahydrofuran, the aqueous solution was acidified (pH 5-6), the white solid formed was collected, washed with water and dried under high vacuum to give the title compound (65 mg, 82%). ¹H-NMR (400 MHz, DMSO₆) δ 7.21 B 7.37 (m, 7H), 7.02 (d, J=2.4 Hz, 1H), 6.78 (dd, J=8.8, 2.4 Hz, 1H), 6.36 (t, J=6.0 Hz, 1H), 6.30 (d, J=2.9 Hz, 1H), 6.06 (t, J=5.7 Hz, 1H), 4.34 (t, J=6.8 Hz, 2H), 4.20 (d, J=6.0 Hz, 2H), 3.96 (t, J=6.2 Hz, 2H), 3.19 (q, J=6.4 Hz, 2H), 2.71 (t, J=6.8 Hz, 2H), 1.83 (t, J=6.5 Hz, 2H). Mass spectrum (LCMS, ESI) Calcd. for C₂₂H₂₅N₃O₄396.4 (M+H), found: 396.1.

EXAMPLE 11

3-[5-(2-5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl-acetylamino)-indol-1-yl]-hexanoic acid

a) (5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-acetic acid

To a solution of 7-ethoxycarbonylmethyl-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester (1.0 g, 3.12 mmol) in methanol (10 mL) was added a solution of NaOH (0.15 g, 3.75 mmol) in H₂O (1.0 mL), and stirred at ambient temperature overnight. After evaporating the solvents, the resulting mixture was acidified to pH 3-4 with 1 N HCl, and extracted with EtOAc (3 times). The extracts were combined, washed with brine, dried over sodium sulfate, concentrated and flash chromatographed on silica gel, eluting with MeOH/DCM (1, 2.5, and 5%) to give the desired acid (0.57 g, 63% yield) as a yellow solid. The solid (0.57 g, 1.95 mmol) was dissolved DCM (5.0 mL), and TFA added (0.45 mL). After stirring at ambient temperature overnight, additional TFA (0.9 mL) was added, and the mixture stirred for 24 h. Solvents were evaporated, giving the title compound (0.60 g, quantitative yield) as a yellow solid. Mass Spectrum (LCMS, ESI) calculated for C₁₀N₁₃N₂O₂193.1 (M+H); found 193.2.

b) 3-(5-Nitro-indol-1-yl)-hexanoic acid ethyl ester

The title compound was synthesized from 5-nitroindole using the procedure described in Example 2, step (c), in 34% yield as an orange oil. Mass spectrum (LCMS, ESI) calculated for C₁₆H₂₁N₂O₄305.3 (M+H); found 305.2.

c) 3-(5-Amino-indol-1-yl)-hexanoic acid ethyl ester

A mixture of 3-(5-nitro-indol-1-yl)-hexanoic acid ethyl ester (1.49 g, 4.9 mmol), and 10% palladium on activated carbon (149 mg) in ethanol (15 mL) was hydrogenated in a hydrogen balloon for 2 days. The mixture was filtered through Celite, and the Celite washed with methanol. The filtrate and washing were combined, concentrated, and flash chromatographed on silica gel, eluting with EtOAc/DCM (20, 30%) to afford the title compound (1.05 g, 78% yield) as dark brown oil. ¹H NMR (CDCl₃) δ 7.22 (d, 1H, J=8.7 Hz), 7.05 (d, 1H, J=3.2 Hz), 6.90 (d, 1H, J=2.3 Hz), 6.66 (dd, 1H, J=2.2, 8.7 Hz), 6.33 (d, 1H, J=3.2 Hz), 4.78-4.73 (m, 1H), 4.02-3.96 (m, 2H), 3.47 (bs, 2H), 2.87-2.74 (m, 2H), 1.94-1.87 (m, 1H), 1.84-1.77 (m, 1H), 1.27-1.09 (m, 2H), 1.08 (t, 3H, J=7.1 Hz), 0.85 (t, 3H, J=7.3 Hz).

d) 3-[5-(2-5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl-acetylamino)-indol-1-yl]-hexanoic acid ethyl ester

A solution of (5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-acetic acid (0.2 g, 0.65 mmol), 3-(5-amino-indol-1-yl)-hexanoic acid ethyl ester (0.19 g, 0.71 mmol), BOP (0.35 g, 0.78 mmol), and diisopropylethylamine (0.45 mL, 2.6 mmol) in DMF (2.5 mL) was stirred for 16 h. Solvents were evaporated. The resulting residue was partitioned between H₂O and EtOAc. The aqueous layer was separated and extracted once more with EtOAc. The extracts were combined, washed with H₂O, brine, dried over Na₂SO₄, concentrated, and flash chromatographed on silica gel, eluting with EtOAc/DCM (15, 30, 50, and 80%) to give the title compound (0.20 g, 67% yield) as a brown oil. Mass spectrum (LCMS, ESI) calculated for C₂₆H₃₃N₄O₃449.3 (M+H); found 449.3.

e) 3-[5-(2-5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl-acetylamino)-indol-1-yl]-hexanoic acid

To a solution of 3-[5-(2-5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl-acetylamino)-indol-1-yl]-hexanoic acid ethyl ester (50 mg, 0.12 mmol) in THF (1.0 mL) was added a solution of NaOH (18 mg, 0.45 mmol) in H₂O (0.15 mL), and stirred at ambient temperature for 14 h. Solvents were evaporated. To the resulting residue was added 1N HCl until the solution reached a pH of 5. The mixture was extracted with EtOAc/DCM (9:1) several times until the aqueous layer was free from product by TLC. The extracts were combined, dried over Na₂SO₄, concentrated, and flash chromatographed on silica gel, eluting with MeOH/DCM (2.5, 5, and 7.5%) to afford the title compound (38 mg, 81% yield) as a pale brown solid. ¹H NMR (CDCl₃) δ 7.79 (d, 1H, J=1.9 Hz), 7.57 (d, 1H, J=7.3 Hz), 7.45 (d, 1H, J=8.9 Hz,), 7.34 (d, 1H, J=3.2 Hz), 7.23 (dd, 1H, J=2.0, 8.8 Hz), 6.69 (d, 1H, J=7.3 Hz), 6.46 (d, 1H, J=3.2 Hz), 3.83 (s, 0.9H), 3.81 (s, 0.5H), 3.49 (t, 2H, J=5.6 Hz), 2.89 (d, 2H, J=7.2 Hz), 2.83 (t, 2H, J=6.1 Hz), 2.67-1.86 (m, 4H), 1.19-0.99 (m, 2H), 0.86 (t, 3H, J=7.3 Hz). Mass spectrum (LCMS, ESI) calculated for C₂₄H₂₉N₄O₃421.2 (M+H); found 421.3.

EXAMPLE 12

3-(5-{2-[N-(4,5-Dihydro-1H-imidazol-2-yl)-aminooxy]-ethoxy}-indol-1-yl)-3-phenyl-propionic acid

a) 5(2-Benzyloxy-ethoxy)-2-nitro-toluene

3-Methyl-4-nitrophenol (8.75 g, 57.1 mmol), 2-benzyloxyethanol (8.70 g, 57.1 mmol) and triphenylphosphine (22.5 g, 85.1 mmol) were dissolved in tetrahydrofuran (200 mL). The mixture was placed under argon at 0° C. and stirred for 10 minutes. Diisopropylazodicarboxylate (17.3 g, 58.1 mmol) was added all at once. The reaction was stirred overnight (16 h). The solvent was removed under vacuum, and the crude mixture was purified via column chromatography to give the product with reduced diisopropylazodicarboxylate impurities. The impurities were eliminated via crystallization with hexane/ethyl acetate. The crystals were filtered and the mother liquid was concentrated under vacuum to afford the title compound (12.36 g, 75%) as oil. ¹H NMR (CDCl₃), δ 8.08 (d, 1H, J=9.7 Hz), 7.31-7.38 (m, 3H), 6.82 (m, 2H), 4.65 (s, 2H), 4.23 (t, 2H, J=4.9 Hz), 3.87 (t, 2H, J=4.9 Hz), 2.63 (s, 3H).

b) 5-(2-Benzyloxy-ethoxy)-1H-indole

5(2-Benzyloxy-ethoxy)-2-nitro-toluene (12.4 g, 43.0 mmol), N—N-dimethylfomamide dimethyl acetal (6.55 g, 51.6 mmol) and pyrrolidine (3.68 g, 51.6 mmol) were dissolved in N—N-dimethylfomamide (25 mL). The mixture was heated to 120° C. for 16 h. The solvent was evaporated under vacuum and the crude reaction was dissolved in 70% ethyl acetate/methanol (250 mL). The reaction was placed in a Parr Hydrogenator under a hydrogen atmosphere for 16 h with 10% palladium on carbon [10% w/w] (3.00 g) at 50 psi. The reaction was filtrated over celite and the crude mixture was purified via column chromatography with silica gel eluting with hexane/ethyl acetate to give the title compound (22% yield). ¹H NMR (CDCl₃) δ 7.27-7.41 (m, 6H), 7.19 (t, 1H, J=2.5 Hz), 7.13 (d, 1H, J=2.3 Hz), 6.91 (dd, 1H, J=2.5, 8.8 Hz), 6.48 (m, 1H), 4.67 (s, 2H), 4.21 (m, 2H), 3.87 (m, 2H).

c) 3-[5-(2-Benzyloxy-ethoxy)-indol-1-yl]-3-phenyl-acrylic acid ethyl ester

5-(2-Benzyloxy-ethoxy)-1H-indole (2.20 g, 8.20 mmol) and ethyl phenyl propiolate (1.72 g, 9.80 mmol) were dissolved in tetrahydrofuran (5 mL) under an argon atmosphere. Tetrabutylammonium flouride [1M in THF] (20.5 ml, 20.5 mmol) was added at once and the reaction was heated at 70° C. for 16 hr. The reaction was extracted with a mixture of ethyl acetate and brine. The organic layer was collected, dried (Na₂SO₄), filtered and evaporated under vacuum to give a crude mixture, which was purified via column chromatography with silica gel, eluting with hexane/ethyl acetate to give the title compound (69% yield) as an E/Z isomeric mixture. ¹H NMR (CDCl₃), δ 7.30-7.53 (m, 10.7H), 7.09-7.13 (m, 2H), 6.97 (d, 0.3H, J=3.2 Hz), 6.78 (m, 1H), 6.61 (d, 0.7H, J=3.9 Hz), 6.24 (s, 0.7H), 6.17 (s, 0.3H), 4.67 (s, 2H), 4.20 (m, 2H), 4.14 (c, 0.6H, J=7.2 Hz), 4.06 (c, 1.4H, J=7.2 Hz), 3.87 (m, 2H), 1.18 (t, 0.9H, J=6.9 Hz), 1.05 (t, 2.1H, J=7.2 Hz).

d) 3-[5-(2-Hydroxy-ethoxy)-indol-1-yl]-3-phenyl-propionic acid ethyl ester

3-[5-(2-benzyloxy-ethoxy)-indol-1-yl]-3-phenyl-acrylic acid ethyl ester (2.5 g, 5.6 mmol) was dissolved in 70% ethyl acetate/methanol (50 mL) and added under an argon atmosphere to a suspension of 10% palladium on carbon [10% w/w] (3.0 g) in the same solvent (50 mL). The reaction was placed in a Parr Hydrogenator for 6 h. The reaction was filtered through celite and the solvent was evaporated under vacuum. Purification of the crude mixture via column chromatography with silica gel, eluting with hexane/ethyl acetate gave the title compound (80% yield). ¹H NMR (CDCl₃), δ 7.14-7.32 (m, 7H), 6.82 (dd, 1H, J=2.3, 8.8 Hz), 6.45 (d, 1H, J=3.0 Hz), 6.07 (d, 1H, J=2.1 Hz), 6.01 (t, 1H, J=7.4 Hz), 4.03 (m, 4H), 3.91 (m, 2H), 3.27 (m, 2H), 1.06 (t, 3H, J=7.2 Hz).

e) 3-{5-[2-(1,3-Dioxo-1,3-dihydro-isoindol-2-yloxy)-ethoxy]-indol-1-yl}-3-phenyl-propionic acid ethyl ester

3-[5-(2-hydroxy-ethoxy)-indol-1-yl]-3-phenyl-propionic acid ethyl ester (0.77 g, 2.10 mmol), N-hydroxyphthalimide (0.40 g, 2.40 mmol) and triphenylphosphine (0.85 g, 3.24 mmol) were dissolved in tetrahydrofuran (5 mL). The mixture was placed under an argon atmosphere at 0° C. and stirred for 10 minutes. Diisopropylazodicarboxylate (0.65 g, 3.24 mmol) was added all at once. After stirring overnight (16 h), the solvent was removed under vacuum, and the crude mixture was purified via column chromatography to afford the title compound (96% yield). ¹H NMR (CDCl₃) δ 7.79 (m, 2H), 7.70 (m, 2H), 7.15-7.29 (m, 7H), 7.03 (d, 1H, J=2.3 Hz), 6.69 (dd, 1H, J=2.5, 9.0 Hz), 6.43 (d, 1H, J=3.7 Hz), 5.99 (t, 1H, J=7.7 Hz), 4.56 (m, 2H), 4.34 (m, 2H), 4.02 (c, 2H, J=7.2 Hz), 3.27 (m, 2H), 1.06 (t, 3H, J=7.2 Hz).

f) 3-[5-(2-Aminooxy-ethoxy)-indol-1-yl]-3-phenyl-propionic acid ethyl ester

3-{5-[2-(1,3-dioxo-1,3-dihydro-isoindol-2-yloxy)-ethoxy]-indol-1-yl}-3-phenyl-propionic acid ethyl ester (1.0 g, 2.0 mmol) was dissolved in tetrahydrofuran (4 mL) at room temperature. Dimethylamine [1.0 M in THF] (10 mL, 10 mmol) was added and the reaction stirred at room temperature for 16 h. The solvent was evaporated under vacuum and the crude mixture was purified via column chromatography with silica gel to afford the title compound (73% yield). ¹H NMR (CDCl₃) δ 7.15-7.28 (m, 7H), 7.08 (d, 1H, J=2.5 Hz), 6.84 (dd, 1H, J=2.3, 8.8 Hz), 6.45 (d, 1H, J=2.3 Hz), 6.00 (t, 1H, J=7.7 Hz), 4.56 (m, 2H), 4.15 (m, 2H), 4.00 (m, 4H), 3.26 (m, 2H), 1.06 (t, 3H, J=7.2 Hz).

g) 3-(5-{2-[N-(4,5-Dihydro-1H-imidazol-2-yl)-aminooxy]-ethoxy}-indol-1-yl)-3-phenyl-propionic acid ethyl ester

3-[5-(2-aminooxy-ethoxy)-indol-1-yl]-3-phenyl-propionic acid ethyl ester (208 mg, 0.56 mmol) and 2-(3,5-dimethylpyrazolyl)-4,5-dihydroimidizole hydrobromide (125 mg, 0.90 mmol) were dissolved in methanol (3 mL) and stirred for 5 days. The solvent was evaporated under vacuum and the crude mixture was purified via column chromatography with silica gel, eluting with 10% methanol in dichloromethane to afford the title compound (99% yield). ¹H NMR (CDCl₃) δ 7.16-7.29 (m, 7H), 7.07 (d, 1H, J=2.3 Hz), 6.80 (dd, 1H, J=2.3, 8.8 Hz), 6.47 (d, 1H, J=3.2 Hz), 6.00 (t, 1H, J=7.0 Hz), 4.24 (m, 2H), 4.17 (m, 2H), 4.03 (c, 2H, J=7.2 Hz), 3.51 (br s, 4H), 3.26 (m, 2H), 1.09 (t, 3H, J=7.2 Hz).

h). 3-(5-{2-[N-(4,5-Dihydro-1H-imidazol-2-yl)-aminooxy]-ethoxy}-indol-1-yl)-3-phenyl-propionic acid

3-(5-{2-[N-(4,5-dihydro-1H-imidazol-2-yl)-aminooxy]-ethoxy}-indol-1-yl)-3-phenyl-propionic acid ethyl ester (0.24 g, 0.55 mmol) was dissolved in 70% methanol/water (4 mL). Lithium hydroxide monohydratate (0.70 g, 4.67 mmol) was added and the reaction was stirred for 16 h at room temperature under an argon atmosphere. The solution was neutralized with 1.0 N HCl (4.67 mL) and the solvent was evaporated under vacuum. The crude mixture was purified via column chromatography with silica gel, eluting with 10% methanol/dichloromethane to afford the title compound (74% yield). ¹H NMR (DMSO-d6) δ 7.68 (d, 1H, J=3.2 Hz), 7.42 (d, 1H, J=9.0 Hz), 7.20-7.34 (m, 5H), 7.05 (d, 1H, J=2.5 Hz), 6.75 (dd, 1H, J=2.5, 9.0 Hz), 6.40 (d, 1H, J=3.0 Hz), 5.96 (m, 1H), 4.16 (m, 4H), 3.59 (br s, 4H), 3.36 (m, 2H). Mass Spectrum (LCMS, ESI) calculate for C₂₂H₂₅N₄O₄409.2 (M+H); found 409.2.

EXAMPLE 13

3-(5-{2-[Guanidino-oxy]-ethoxy}-indol-1-yl)-3-phenyl-propionic acid

a) 3-(5-{2-[Guanidino-oxy]-ethoxy}-indol-1-yl)-3-phenyl-propionic acid ethyl ester

3-[5-(2-aminooxy-ethoxy)-indol-1-yl]-3-phenyl-propionic acid ethyl ester (0.28 g, 0.75 mmol) and 1H-pyrazole-1-carboxamide hydrochloride (0.99 g, 0.67 mmol) were dissolved in methanol (3 mL) and stirred for 5 days. The solvent was evaporated under vacuum and the crude mixture was purified via column chromatography with silica gel, eluting with 10% methanol/dichloromethane to afford the title compound (97% yield). ¹H NMR (CDCl₃), δ 7.11-7.26 (m, 7H), 7.00 (d, 1H, J=2.3 Hz), 6.75 (dd, 1H, J=2.3, 8.8 Hz), 6.43 (d, 1H, J=3.2 Hz), 5.98 (t, 1H, J=7.6 Hz), 4.08 (m, 2H), 3.99 (m, 4H), 3.23 (m, 2H), 1.05 (t, 3H, J=7.2 Hz).

b) 3-(5-{2-[Guanidino-oxy]-ethoxy}-indol-1-yl)-3-phenyl-propionic acid

3-(5-{2-[guanidino-oxy]-ethoxy}-indol-1-yl)-3-phenyl-propionic acid ethyl ester (0.30 gg, 0.71 mmol) was dissolved in 70% methanol/water (4 mL) and lithium hydroxide monohydratate (0.70 g, 4.67 mmol) was added. The reaction was stirred for 16 h at room temperature under an argon atmosphere. The solution was neutralized with 1.0 N HCl (4.67 mL) and the solvent was evaporated under vacuum. The crude mixture was purified via column chromatography with silica gel, eluting with 10% methanol/dichloromethane to afford the title compound (80% yield). ¹H NMR (CD₃OD-d4) δ 7.34 (d, 1H, J=3.2 Hz). 7.06-7.15 (m, 6H), 6.97 (d, 1H, J=2.3 Hz), 6.65 (dd, 1H, J=2.5, 9.0 Hz), 6.31 (d, 1H, J=3.2 Hz), 5.93 (t, 1H, J=7.0 Hz), 4.01 (m, 4H), 3.07 (m, 2H).

EXAMPLE 14

3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-hexanoic acid

a) 3-(5-Benzyloxy-indol-1-yl)-hexanoic acid ethyl ester

A solution of 5-benzyloxyindole (5.00 g, 22.4 mmol) in DMF (20 mL) was added dropwise to a stirred solution of sodium hydride (0.91 g, 38.1 mmol) in DMF (50 mL) at 0° C. and stirred for 20 minutes. Ethyl-1-bromocaproate (5.59 g, 26.9 mmol) in DMF (20 mL) was added and the reaction was stirred at room temperature overnight. The reaction was then poured into cold H₂O (150 mL) and extracted with ethyl acetate (3×50 mL), dried over magnesium sulfate and concentrated. The residue was purified by flash chromatography on silica gel (40% ethyl acetate in hexane) to give the title compound as oil (40% yield). ¹H NMR (CDCl₃) δ 7.47 (d, 2H, J=7.2 Hz), 7.38 (t, 2H, J=7.1 Hz), 7.32 (d, 2H, J=8.8 Hz), 7.12 (dd, 2H, J=2.4, 13.7 Hz), 6.94 (dd, 1H, J=2.4, 10.2 Hz), 6.44 (d, 1H, J=3.1 Hz), 5.09 (s, 2H), 4.79 (m, 1H), 3.98 (q, 2H, J=7.1 Hz), 2.80 (m, 2H), 1.90 (m, 2H), 1.25 (m, 2H), 1.07 (t, 3H, J=7.2 Hz), 0.86 (t, 3H, J=7.3 Hz).

b) 3-(5-Hydroxy-indol-1-yl)-hexanoic acid ethyl ester

Palladium (0) on carbon [10% w/w] (0.20 g) was added to a solution of (5-benzyloxy-indol-1-yl)-hexanoic acid ethyl ester (2.00 g, 5.47 mmol) in methanol (10 mL) under an argon atmosphere. The reaction was placed under H₂atmosphere and stirred overnight. The solution was filtered through a bed of celite and concentrated. The residue was purified by flash chromatography on silica gel (10% ethyl acetate in hexane) to give the title compound as a solid (93% yield). ¹H NMR (CDCl₃) δ 7.30 (d, 2H, J=8.8 Hz), 7.12 (m, 1H), 7.00 (m, 1H), 6.75 (m, 1H), 6.45 (m, 1H), 4.80 (m, 1H), 4.72 (s, 1H), 3.95 (q, 2H, J=7.2 Hz), 2.80 (m, 2H), 1.84 (m, 2H), 1.28 (m, 2H), 1.10 (t, 3H, J=7.2 Hz), 0.90 (t, 3H, J=7.2 Hz).

c) 7-{2-[1-(1-Ethoxycarbonylmethyl-butyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

Triphenylphosphine (0.20 g, 0.77 mmol) was added to a solution of 7-(2-hydroxy-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester (0.16 g, 0.58 mmol) and 3-(5-hydroxy-indol-1-yl)-hexanoic acid ethyl ester (0.10 g, 0.38 mmol) in THF (4 mL) at 0° C. Diisopropylazodicarboxylate (0.15 g, 0.77 mmol) was added dropwise and the reaction was stirred at room temperature overnight. The solution was then concentrated. The residue was purified by flash chromatography on silica gel (30% ethyl acetate in hexane) to give the title compound (13% yield). ¹H NMR (CDCl₃) δ 7.30 (m, 2H), 7.09 (m, 2H), 6.96 (d, 1H, J=7.6 Hz), 6.86 (m, 1H), 6.41 (d, 1H, J=3.2 Hz), 4.78 (m, 1H), 4.39 (t, 2H, J=6.9 Hz), 3.98 (q, 2H, J=7.1 Hz), 3.75 (m, 2H), 3.21 (t, 2H, J=6.9 Hz), 2.86 (m, 2H), 2.77 (m, 2H), 1.85 (m, 4H), 1.50 (s, 9H), 1.20 (m, 2H), 1.06 (t, 3H, J=7.1 Hz), 0.85 (t, 3H, J=7.4 Hz).

d) 3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-hexanoic acid ethyl ester

7-{2-[1-(1-Ethoxycarbonylmethyl-butyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester (0.02 g, 0.04 mmol) was heated neat to 200° C. for 15 minutes. The residue was purified by flash chromatography on silica gel (ethyl acetate) to give the title compound (90% yield). ¹H NMR (CDCl₃) δ 7.28 (m, 1H), 7.08 (m, 3H), 6.86 (m, 1H), 6.48 (d, 1H, J=8.0 Hz), 6.41 (d, 1H, J=4.0 Hz), 4.84 (s, 1H), 4.78 (m, 1H), 4.29 (t, 2H, J=4.0 Hz), 3.98 (q, 2H, J=8.0 Hz), 3.39 (m, 2H), 3.04 (t, 2H, J=8.0 Hz), 2.81 (m, 2H), 2.69 (t, 2H, J=8.0 Hz), 1.93 (m, 4H), 1.25 (m, 2H), 1.10 (t, 3H), 0.85 (t, 3H).

e) 3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-hexanoic acid

Sodium hydroxide (0.01 g, 0.23 mmol) was added to a solution of 3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-hexanoic acid ethyl ester (0.02 g, 0.04 mmol) in methanol/water (9/1, 1 mL) and stirred overnight. The reaction was acidified to pH 6 with 1N HCl and the crude product was extracted with ethyl acetate (3×10 mL) and concentrated. The residue was purified by flash chromatography on silica gel (10% methanol in ethyl acetate) to give the title compound (28% yield). ¹H NMR (CDCl₃) δ 10.4 (bs, 1H), 7.38 (d, 1H, J=8.0 Hz), 7.21 (d, 1H, J=3.2 Hz), 7.14 (d, 1H, J=8.0 Hz), 7.00 (d, 1H, J=2.3 Hz), 6.73 (m, 1H), 6.48 (d, 1H, J=7.3 Hz), 6.32 (d, 2H, J=3.0 Hz), 4.80 (s, 1H), 4.22 (t, 2H, J=7.0 Hz), 3.47 (m, 2H), 2.95 (t, 2H, J=6.8 Hz), 2.68 (m, 4H), 1.88 (m, 4H), 1.15 (m, 2H), 0.81 (t, 3H, J=7.4 Hz). Mass Spectrum (LCMS, ESI) calculated for C₂₄H₃₀N₃O₃408.2 (M+H); found 408.3.

EXAMPLE 15

3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

a) 7-{2-[1-(2-Ethoxycarbonyl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-(2-hydroxy-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester and ethyl 2-(5-hydroxyindolyl)propanoate using the procedure described in Example 14, step (c), in 20% yield. ¹H NMR (CDCl₃) δ 7.32 (d, J=7.62 Hz, 1H), 7.21 (d, J=8.87 Hz, 1H), 7.11 (d, 1H, J=2.3 Hz), 7.08 (d, 1H, J=3.10 Hz), 6.95 (d, 1H, J=7.60 Hz), 6.88 (dd, 1H, J=2.4, 8.9 Hz), 6.37 (dd, 1H, J=0.6, 3.1 Hz), 4.42-4.36 (m, 2H), 4.11 (q, 2H, J=7.2 Hz), 3.76 (dd, 2H, J=6.0, 7.2 Hz), 3.21 (t, 2H, J=6.9 Hz), 2.79 (t, 2H, J=6.9 Hz), 2.73 (t, 2H, J=6.7 Hz), 1.92 (p, 2H, J=6.6 Hz), 1.52 (s, 9H), 1.20 (t, 3H, J=7.1 Hz).

b) 3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester

The title compound was synthesized from 7-{2-[1-(2-ethoxycarbonyl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 14, step (d), in 50% yield. ¹H NMR (CDCl₃) δ 7.21 (d, 1H, J=8.9 Hz), 7.11-7.07 (m, 3H), 6.88 (dd, 1H, J=2.4, 8.8 Hz), 6.48 (d, 1H, J=7.3 Hz), 6.37 (dd, 1H, J=0.7, 3.1 Hz), 4.81 (bs, 1H), 4.40 (t, 2H, J=6.9 Hz), 4.30 (t, 2H, J=7.3 Hz, 2H), 4.11 (q, 2H, J=7.1 Hz), 3.42-3.38 (m, 2H), 3.42-3.38 (m, 2H), 3.04 (t, 2H, J=7.0 Hz), 2.79 (t, 2H, J=6.9 Hz), 2.70 (t, 2H, J=6.3 Hz), 1.94-1.88 (m, 2H), 1.20 (t, 3H, J=7.2 Hz). Mass spectrum (LCMS, ESI) calculated for C₂₃H₂₈N₃O₃394.2 (M+H); found 394.3.

c) 3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

The title compound was synthesized from 3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester using the procedure described in Example 14, step (e), in 99% yield. ¹H NMR (CDCl₃) δ 8.83 (bs, 1H), 7.30-7.27 (m, 1H), 7.18 (d, 1H, J=8.9 Hz), 7.16 (d, 1H, J=3.1 Hz), 7.01 (d, 1H, J=2.3 Hz), 6.77 (dd, 1H, J=2.3, 8.8 Hz), 6.50 (d, 1H, J=7.3 Hz), 6.31 (d, 1H, J=3.0 Hz), 4.33 (t, 2H, J=6.8 Hz), 4.25 (t, 2H, J=5.8 Hz), 3.42 (t, 2H, J=5.4 Hz), 3.11 (t, 2H, J=5.8 Hz), 2.76 (t, 2H, J=6.7 Hz), 2.70 (t, 2H, J=6.1 Hz), 1.87 (p, 2H, J=6.1 Hz). Mass Spectrum (LCMS, ESI) calculated for C₂₁N₂₄N₃O₃366.2 (M+H); found 366.3.

EXAMPLE 16

3-Phenyl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

a) 7-(2-Hydroxy-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

7-Ethoxycarbonylmethyl-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester (synthetic methodology described in Published International Patent Appl. WO 00/33838) (6.11 g, 19.0 mmol) was dissolved in tetrahydrofuran (40 mL) at room temperature. The solution was place under argon. Lithium borohydride [2M in tetrahydrofuran] (22.8 mmol, 11.43 mL) was carefully added and the reaction was refluxed overnight (16 h). The mixture was poured into a solution of saturated ammonium chloride and extracted with ethyl acetate. The organic layer was dried over Na₂SO₄, filtered, and evaporated under vacuum to give a crude mixture, which was purified via column chromatography to give 7-(2-hydroxy-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester (49% yield). ¹H NMR(Cl₃CD), δ: 7.30 (d, 1H, J=7.6 Hz), 7.76(d, 1H, J=7.6 Hz), 3.98 (m, 2H), 3.78 (m, 2H), 2.92 (m, 2H), 2.71 (m, 2H), 1.92(m, 2H), 1.54 (s, 9H).

b) 7-[2-(3-Methyl-4-nitro-phenoxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

An ice-cooled solution of 7-(2-hydroxy-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester (100 g, 359 mmol), 3-methyl-4-nitrophenol (45.7 g, 298 mmol), and triphenylphosphine (157 g, 597 mmol) in anhydrous THF (1.5 L) was stirred under an atmosphere of nitrogen for 15 min. To this solution was added diisopropylazodicarboxylate (118 mL, 597 mmol) over 5 minutes and the mixture was allowed to gradually warm up and stir at room temperature for 16 h. The mixture was filtered to remove the insoluble material and the filtrate was concentrated in vacuo and re-dissolved in diethyl ether (1 L) to remove most of the reduced diisopropylazodicarboxylate and triphenylphosphine oxide (125 g) by filtration. The ether solution was concentrated in vacuo to give a gum (286 g) as the crude product. The crude product was filtered through a plug of silica gel (1 Kg) using 2:1 ether/pet-ether as eluent to removed the remaining triphenylphosphine. The fractions from the plug were combined and concentrated to 1 L, which resulted in the crystallization of the title compound (91 g, 61% yield). ¹H NMR (CDCl₃), δ 8.05 (d, 1H, J=8.8 Hz), 7.33 (d, 1H, J=7.6 Hz), 6.89 (d, 1H, J=7.6 Hz), 6.81 (m, 2H), 4.44 (t, 2H, J=8.00 Hz), 3.76 (m, 2H), 3.20 (t, 2H, J=8.00 Hz), 2.73 (m, 2H), 2.61 (s, 3H), 1.93 (m, 2H), 1.51 (s, 9H).

c) 7-[2-(1H-Indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

Treatment of 7-[2-(3-methyl-4-nitro-phenoxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester (14.0 g, 33.9 mmol) with N,N-dimethylformamide dimethyl acetal (5.40 mL, 40.7 mmol) and pyrrolidine (3.37 mL, 40.7 mmol) in DMF (20 mL) at 75° C. gave a deep orange solution after 16 h. After that period the solvent was removed under vacuum to yield a red gum (15.5 g) corresponding with 7-{2-[4-Nitro-3-(2-pyrrolidin-1-yl-vinyl)-phenoxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester. This compound was used in the next step without further purification. 15.5 g of crude 7-{2-[4-Nitro-3-(2-pyrrolidin-1-yl-vinyl)-phenoxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester was dissolved in a 9:1 solution of EtOAc/MeOH in a Parr bottle. After evacuating and purging the solution with nitrogen, palladium on carbon [10% w/w] (1.52 g) was added and the mixture was shaken under an atmosphere of hydrogen at 50 psi overnight (16 h). The mixture was filtered through Celite and washed with methanol. The filtrate was concentrated in vacuo to afford a brown gum (15.8 g). The crude product was purified by column chromatography (SiO₂, 4:1 to 2:1 heptane/ethyl acetate) to give 7-[2-(1H-Indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester as a gray solid contaminated with an impurity. The solid washed with 1:1 ether/pet-ether (30 mL) to give the title compound (7.14 g, 54% yield) as a pure white solid. ¹H NMR (CDCl₃) δ 7.31 (d, 1H, J=7.6 Hz), 7.26 (d, 1H, J=8.8 Hz), 7.17 (m, 1H), 7.13 (d, 1H, J=2.4 Hz), 6.96 (d, 1H, J=7.6 Hz), 6.85 (dd, 1H, J=2.4, 8.8 Hz), 6.45 (m, 1H), 4.38 (t, 2H, J=8.00 Hz), 3.76 (m, 2H,), 3.22 (t, 2H, J=8.00 Hz), 2.73 (m, 2H), 1.93 (m, 2H), 1.51 (s, 9H).

d) 7-{2-[1-(2-Ethoxycarbonyl-1-phenyl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

Method d1
CsF (15.2 g, 100 mmol) was added to a solution of 7-[2-(1H-indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester (20.0 g, 50.8 mmol) in anhydrous DMF (50 mL). Ethyl phenylpropionate (16.5 mL, 100 mmol) was added to the mixture at room temperature and the solution was allowed to stir under a nitrogen atmosphere at 60° C. for 4 h. The mixture was diluted with water (1 L), the crude mixture was dissolved in ethyl acetate (500 mL), washed with water, then brine, dried and concentrated under vacuum to give a yellow gum as crude product. Purification of the crude mixture on silica gel (1:1 pet-ether/ether) gave the title compound as a bright yellow solid (25.5 g, 88% yield), a mixture of E/Z isomers.
Method d2
A mixture of 7-[2-(1H-Indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester (5.00 g, 12.7 mmol), phenyl-propionic acid ethyl ester (4.43 g, 25.4 mmol), and tetrabutylammonium fluoride [1.0 M in THF] (31.8 mL, 31.8 mmol) was stirred at 75° C. for 3 days. After removal of solvent, the crude reaction mixture was submitted to flash chromatography on silica gel (ethyl acetate/hexane, 1:4) to give the title compound (4.66 g, 78% yield) as an E/Z mixture. ¹H NMR (CDCl₃) [E/Z mixture] δ 7.50-7.30 (m, 7H), 7.20 (m, 2H), 7.05 (m, 1H), 6.80 (m, 1H), 6.56 (m, 1H), 6.29 (s, 0.5H), 6.19 (s, 0.5H), 4.44 (m, 2H), 4.07 (q, 2H, J=6.8 Hz), 3.82 (m, 2H), 3.31 (m, 2H), 2.70 (m, 2H), 1.98 (m, 2H), 1.54 (s, 9H), 1.13 (t, 1.5H, J=7.2 Hz), 1.01 (t, 3H, J=7.2 Hz).

e) 7-{2-[1-(2-Ethoxycarbonyl-1-phenyl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

10% Palladium on activated carbon (0.06 g) was added to 7-{2-[1-(2-ethoxycarbonyl-1-phenyl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester (0.50 g, 0.88 mmol) in methanol (10 mL) under argon. The solution was exposed to a hydrogen atmosphere (50 psi) using a Parr shaker for 24 h. The reaction was filtered through celite and washed with methanol. The filtrate was concentrated in vacuo to yield the title compound (0.48 g, 98%). ¹H NMR (CDCl₃) δ 7.15-7.36 (m, 9H), 6.96 (d, 1H, J=7.6 Hz), 6.83 (dd, 1H, J=2.3, 6.6 Hz), 6.46 (d, 1H, J=3.0 Hz), 6.06 (d, 1H, J=7.6 Hz), 4.39 (m, 2H), 4.06 (q, 2H, J=7.1 Hz), 3.78 (m, 2H), 3.31-3.20 (m, 4H), 2.75 (m, 2H), 1.94 (m, 2H), 1.54 (s, 9H), 1.11 (t, 3H, J=7.1 Hz).

f) 3-Phenyl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester

A solution of 7-{2-[1-(2-ethoxycarbonyl-1-phenyl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester (22.9 g, 40.0 mmol) in a 10:1 mixture of anhydrous toluene/DMF (220 mL) was treated with copper (I) trifluoromethanesulfonate benzene complex [30% w/w] (6.87 g) at 130° C. for 75 minutes. The mixture was cooled to room temperature, diluted with water (200 mL), extracted with dichloromethane (2×300 mL), washed with water, then brine, dried and concentrated under vacuum to give a black gum (26.0 g). Purification with silica gel (1:1 ethyl acetate/heptane) gave the title compound as an off-white solid (16.2 g, 86% yield). ¹H NMR (CDCl₃) δ 7.29-6.98 (m, 10H), 6.73 (m, 1H), 6.36 (m, 1H), 5.94 (t, 1H, J=7.5 Hz), 4.69 (bs, 1H), 4.21 (t, 2H, J=7.0 Hz), 3.96 (q, 2H, J=7.1 Hz), 3.32 (m, 2H), 3.20 (m, 2H), 2.96 (t, 2H, J=7.0 Hz), 2.61 (m, 2H), 1.83 (m, 2H), 1.00 (t, 3H, J=7.2 Hz).

g) 3-Phenyl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

A solution of 3-phenyl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester (2.14 g, 4.49 mmol) in a 2:1:0.2 THF/MeOH/H₂O (67 mL) was treated with lithium hydroxide monohydrate (0.38 g, 9.00 mmol) at room temperature. The reaction was stirred for 20 h. The mixture was diluted with ethyl acetate, acidified to pH 4 (0.5 N HCl), washed with water and brine, dried and concentrated to afford a crude mixture (2.02 g), which was purified by column chromatography with silica gel (39:1 to 29:1 dichloromethane/MeOH) to give the title compound (1.31 g, 66% yield). ¹H NMR (CDCl₃) δ 10.5 (s, 1H), 7.44 (d, 1H, J=3.1 Hz), 7.20-7.00 (m, 7H), 6.76 (m, 1H), 6.53 (dd, 1H, J=2.3, 6.6 Hz), 6.41 (s, 1H), 6.19 (d, 1H, J=7.3 Hz), 6.07 (dd, 1H, J=4.3, 7.1 Hz), 3.68 (m, 1H), 3.52 (m, 1H), 3.33 (m, 3H), 3.25-3.09 (m, 2H), 2.58 (m, 3H), 1.77 (m, 3H). Mass Spectrum (LCMS, ESI) calculated for C₂₇H₂₈N₃O₃442.2 (M+H); found 442.3.

EXAMPLE 17

a) 3-(5-Benzyloxy-indol-1-yl)-3-phenyl-acrylic acid ethyl ester

A mixture of 5-benzyloxy-1H-indole (4.46 g, 20.0 mmol), phenyl-propionic acid ethyl ester (7.00 g, 40.0 mmol), and tetrabutylammonium fluoride [1.0 M in THF] (36.0 mL, 50.0 mmol) was stirred for 3 days. After removal of solvent, the crude reaction mixture was submitted to flash chromatography on silica gel with ethyl acetate/hexane (1:4) to give the title compound (5.42 g, 68% yield) as an E/Z isomeric mixture. Mass Spectrum (LCMS, ESI) calculated for C₂₆H₂₄NO₃398.2 (M+H); found 398.2.

b) 3-(5-Hydroxy-indol-1-yl)-3-phenyl-propionic acid ethyl ester

A mixture of 3-(5-benzyloxy-indol-1-yl)-3-phenyl-acrylic acid ethyl ester (1.94 g, 4.89 mmol) and palladium on carbon [10% w/w] (60 mg) in methanol (25 mL) was stirred under hydrogen atmosphere for 24 h. After removal of the catalyst by filtration, the crude product was purified by flash chromatography on silica gel with hexane/ethyl:acetate (4:1) to give the title compound in 96% yield. ¹H NMR (CDCl₃) δ 7.10-7.30 (m, 7H), 6.99 (d, J=2.4 Hz, 1H), 6.69 (dd, J=2.4 and 8.7 Hz, 1H), 6.39 (d, J=3.2 Hz, 1H), 5.99 (t, J=7.6 Hz, 1H), 5.31 (br, 1H), 4.03 (q, J=7.2 Hz, 2H), 3.26 (m, 2H), 1.08 (t, J=7.2 Hz, 3H). ¹³C NMR (CDCl₃) δ 170.4, 149.7, 139.8, 131.5, 129.3, 128.8, 127.9, 126.2, 125.8, 111.6, 110.6, 105.2, 101.4, 61.1, 56.2, 40.4, 13.9. Mass Spectrum (LCMS, ESI) calculated for C₁₉H₂₀NO₃310.1 (M+H); found 310.1.

c) 7-{2-[1-(2-Ethoxycarbonyl-1-phenyl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized as an E/Z isomeric mixture from 3-(5-hydroxy-indol-1-yl)-3-phenyl-propionic acid ethyl ester using the procedure described in Example 16, step (d2), in 81% yield. Mass Spectrum (LCMS, ESI) calculated for C₂₉H₃₀N₃O₃468.2 (M-Boc+H); found 469.4.

d) 7-{2-[1-(2-Ethoxycarbonyl-1-phenyl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 3-(5-hydroxy-indol-1-yl)-3-phenyl-propionic acid ethyl ester using the procedure described in Example 14, step (b), in 24% yield. ¹H NMR (CDCl₃) δ 7.28 (m, 4H), 7.18 (m, 4H), 7.09 (d, J=2.9 Hz, 1H), 6.93 (d, J=7.7 Hz, 1H), 6.80 (dd, J=2.5 and 7.9 Hz, 1H), 6.43 (dd, J=0.6 and 3.2 Hz, 1H), 6.00 (m, 1H), 4.35 (t, J=6.9 Hz, 2H), 4.02 (q, J=7.0 Hz, 2H), 3.75 (m, 2H), 3.26 (q, 2H), 3.19 (t, J=6.9 Hz, 2H), 2.71 (t, J=6.7 Hz, 2H), 1.92 (m, 2H), 1.51 (s, 9H), 1.08 (t, J=7.1 Hz, 3H). Mass Spectrum (LCMS, ESI) calculated for C₃₄H₄₀N₃O₅570.2 (M+H), found 570.0 (M+H).

e) 3-Phenyl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester

The title compound was synthesized from 7-{2-[1-(2-ethoxycarbonyl-1-phenyl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 14, step (d), in 43% yield. ¹H NMR (CDCl₃) δ 7.29 (m, 3H), 7.19 (m, 4H), 7.08 (m, 2H), 6.81 (dd, J=2.4 and 8.9 Hz, 1H), 6.45 (m, 2H), 6.03 (t, 1H), 4.86 (br, 1H), 4.28 (t, J=7.1 Hz, 2H), 4.06 (q, J=6.1 Hz, 2H), 3.39 (m, 2H), 3.27 (m, 2H), 3.04 (t, J=7.0 Hz, 2H), 2.68 (t, J=6.3 Hz, 2H), 1.88 (m, 2H), 1.08 (t, J=7.1 Hz, 3H). Mass Spectrum (LCMS, ESI) calculated for C₂₉H₃₂N₃O₃470.2 (M+H); found 470.3.

f) 3-Phenyl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

The title compound was synthesized from 3-phenyl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester using the procedure described in Example 14, step (e), in 51% yield. ¹H NMR (CDCl₃) δ 10.59 (br, 1H), 7.08-7.27 (m, 8H), 6.84 (d, J=2.4 Hz, 1H), 6.61 (dd, J=2.4 and 8.9 Hz, 1H), 6.48 (m, 1H), 6.26 (d, J=7.3 Hz, 1H), 6.13 (dd, J=4.4 Hz, 1H), 3.59 (m, 1H), 3.17-3.42 (m, 5H), 2.40-2.64 (m, 4H), 1.84 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₇H₂₈N₃O₃442.2 (M+H); found 442.4.

EXAMPLE 18

3-(3-Benzyloxy-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

a) (3-Benzyloxy-phenylethynyl)-trimethyl-silane

To a solution of 3-benzyloxy-1-iodophenyl (8.40 g, 27.0 mmol), trimethylsilyl acetylene (4.21 mL, 29.8 mmol), copper (I) iodide (0.51 g, 2.71 mmol), and triethylamine (8.22 mL, 81.2 mmol) in dichloromethane (30 mL) was added dichlorobis(triphenylphosphine) palladium(II) (0.38 g, 5.41 mmol) portionwise over a 3 minute period, and the reaction mixture stirred overnight. The mixture was then concentrated and the resulting residue was filtered through Celite. The filtrate was concentrated, and purified via column chromatography with silica gel, eluting with dichloromethane/hexane/ethyl acetate (10/1) to give the title compound (98% yield) as pale yellow oil. ¹H NMR (CDCl₃) δ 7.43-7.32 (m, 5H), 7.19 (m, 1H), 7.08 (m, 2H), 6.92 (m, 1H), 5.04 (s, 2H), 0.24 (s, 9H).

b) 3-Benzyloxy-phenylethynyl

Tetrabutylammonium fluoride [1.0 M in THF] (28.0 mL) was added dropwise at room temperature to a solution of (3-benzyloxy-phenylethynyl)-trimethyl-silane (6.40 g, 22.7 mmol) in aqueous THF (30 mL) and the reaction was stirred for 1 hour. Water (100 mL) was added and the crude product was extracted with ethyl acetate (3×50 mL), dried over magnesium sulfate and concentrated. The crude mixture was then purified via column chromatography with silica gel (10% ethyl acetate in hexane) to give the title compound (89% yield). ¹H NMR (CDCl₃) δ 7.42-7.29 (m, 5H), 7.21 (m, 1H), 7.09 (m, 2H), 6.95 (m, 1H), 5.03 (s, 2H), 3.04 (s, 1H).

c) 3-(3-Benzyloxy-phenyl)-3-chloro-acrylic acid ethyl ester

A mixture of ethyl chloroformate (2.90 mL, 26.7 mmol), 3-benzyloxy-phenylethynyl (2.50 g, 8.91 mmol), and carbonylchlorobis-(triphenylphosphine)-rhodium(I) (0.03 g, 0.05 mmol) in toluene (10 mL) was heated under argon at 110° C. for 12 h. The solvent was removed under reduced pressure and the crude product was purified by chromatography on silica gel, eluting with hexane/ethyl acetate (10/1) to give the title compound (50%) as yellow oil. ¹H NMR (CDCl₃) δ 7.48-7.28 (m, 8H), 7.06 (m, 1H), 6.57 (s, 1H), 5.12 (s, 2H), 4.31 (q, 2H, J=7.1 Hz), 1.37 (t, 3H, J=7.1 Hz).

d) 7-(2-{1-[1-(3-Benzyloxy-phenyl)-2-ethoxycarbonyl-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

A solution of 7-[2-(1H-indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester (0.20 g, 0.51 mmol), 7-(2-{1-[1-(3-benzyloxy-phenyl)-2-ethoxycarbonyl-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester (0.18 g, 0.56 mmol), potassium phosphate (0.16 g, 0.77 mmol), 2-dicyclohexylphosphino-2′(N,N-dimethylamino)biphenyl (0.03 g, 0.08 mmol) and tris(dibenzylideneacetone)dipalladium(0) (0.03 mg, 0.08 mmol) in 10% DMF/toluene (3 mL) was heated at 110° C. for 6 days under argon. The reaction was cooled to room temperature and the solvent was removed under reduced pressure. The crude product was purified by column chromatography with silica gel, eluting with hexane/ethyl acetate (4/1) to give the title compound (29%) as an E/Z isomeric mixture. ¹H NMR (CDCl₃) [E/Z mixture] δ 7.36-7.22 (m, 7H), 7.10 (m, 4H), 6.86 (m, 3H), 6.75 (m, 1H), 6.58 (m, 1H), 6.22 (s, 1H), 5.00 (s, 2H), 4.40 (m, 2H), 4.01 (q, 2H, J=7.2 Hz), 3.78 (m, 2H), 3.22 (m, 2H), 2.75 (m, 2H), 1.85 (m, 2H), 1.52 (s, 9H), 1.01 (t, 3H, J=6.8 Hz).

e) 7-(2-{1-[1-(3-Benzyloxy-phenyl)-2-ethoxycarbonyl-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

Samarium (II) iodide [0.1 M in THF] (14.8 mL, 14.8 mmol) was added to a solution of 7-(2-{1-[1-(3-benzyloxy-phenyl)-2-ethoxycarbonyl-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester (0.10 g, 0.15 mmol), hexamethylphosphoramide (0.39 mL, 2.22 mmol) and either ethanol or methanol (10 equivalents) and stirred at room temperature overnight. Saturated ammonium chloride (20 mL) was added to the reaction and the crude product was extracted with ethyl acetate (3×20 mL). The crude mixture was then purified via column chromatography in silica gel (20% ethyl acetate in hexane) to give the title compound (0.50 g, 50% yield). ¹H NMR (CDCl₃) δ 7.28-7.22 (m, 5H), 7.13 (m, 4H), 7.08 (d, 1H, J=3.2 Hz), 6.86 (m, 1H), 6.79-6.69 (m, 4H), 6.35 (d, 1H, J=3.2 Hz), 5.99 (t, 1H, J=7.5 Hz), 4.98 (s, 2H), 4.29 (t, 2H, J=6.9 Hz), 3.96 (q, 2H, J=7.1 Hz), 3.68 (m, 2H), 3.18-3.10 (m, 4H), 2.66 (m, 2H), 1.85 (m, 2H), 1.45 (s, 9H), 1.01 (t, 3H, J=7.1 Hz).

f) 3-(3-Benzyloxy-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester

The title compound was synthesized from 7-(2-{1-[1-(3-benzyloxy-phenyl)-2-ethoxycarbonyl-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (f), in 43% yield. ¹H NMR (CDCl₃) δ 7.36-7.32 (m, 4H), 7.17-7.07 (m, 6H), 6.77 (m, 4H), 6.43 (m, 2H), 5.99 (m, 1H), 4.96 (s, 2H), 4.90 (bs, 1H), 4.28 (m, 2H), 4.03 (q, 2H, J=7.1 Hz), 3.39 (m, 2H), 3.23 (m, 2H), 2.68 (m, 2H), 2.52 (m, 2H), 1.89 (m, 2H), 1.01 (t, 3H, J=7.1 Hz).

g) 3-(3-Benzyloxy-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

A solution of 3-(3-benzyloxy-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester (0.08 g, 0.14 mmol), lithium hydroxide (0.01 g, 0.22 mmol) and THF/methanol/water [2.0/1.0/0.2 mL] (3.2 mL) was microwave at 100° C. for 15 minutes. Saturated ammonium chloride was added (10 mL) and the product was extracted with ethyl acetate (3×10 mL). The crude product was purified via column chromatography eluting with dichloromethane:ethyl acetate (10:1) to give the title compound (25% yield) as white solid. ¹H NMR (CDCl₃) δ 10.5 (bs, 1H), 7.49 (d, 1H, J=3.0 Hz), 7.39-7.22 (m, 6H), 7.16 (m, 1H), 7.08 (m, 1H), 6.80 (m, 4H), 6.60 (dd, 1H, J=2.2, 6.8 Hz), 6.46 (m, 1H), 6.26 (d, 1H, J=7.3 Hz), 6.09 (m, 1H), 5.29 (s, 2H), 3.59 (s, 1H), 3.38 (m, 3H), 3.29-3.14 (m, 2H), 2.60-2.43 (m, 5H), 1.87 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₃₄H₃₄N₃O₄548.3 (M+H); found 548.4.

EXAMPLE 19

3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-p-tolyl-propionic acid

a) 1-Ethynyl-4-methyl-benzene

The title compound was synthesized from commercially available 4-iodotoluene using the procedures outlined in Example 18, step (a) and step (b), in 60% yield overall. ¹H NMR (CDCl₃) δ 7.38 (d, 2H, J=2.4 Hz), 7.12 (d, 2H, J=2.4 Hz), 3.02 (s, 1H), 2.35 (s, 3H).

b) 3-Chloro-3-p-tolyl-acrylic acid ethyl ester

The title compound was synthesized from 1-ethynyl-4-methyl-benzene using the procedure outlined in Example 18, step (c), in 70% yield. ¹H NMR (CDCl₃) δ 7.58 (d, 2H, J=2.4 Hz), 7.21 (d, 2H, J=2.4 Hz), 6.52 (s, 1H), 4.27 (m, 2H), 2.39 (s, 3H), 1.33 (t, 3H, J=7.2 Hz).

c) 7-{2-[1-(2-Ethoxycarbonyl-1-p-tolyl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 3-chloro-3-p-tolyl-acrylic acid ethyl ester and 7-[2-(1H-indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure outlined in Example 18, step (d), in a 36% yield of E/Z isomeric mixture. ¹H NMR (CDCl₃) [E/Z mixture] δ 7.33 (m, 1.4H), 7.17 (m, 4.6H), 6.96 (m, 1.6H), 6.74 (m, 2.4H), 6.57 (d, 0.8H, J=0.8 Hz), 6.50 (d, 0.2H, J=0.8 Hz), 6.20 (s, 0.8H), 6.11 (s, 0.2H), 4.39 (t, 2H, J=8.0), 4.01 (m, 2H), 3.78 (t, 2H, J=4.0), 3.22 (t, 2H, J=8.0), 2.75 (t, 2H, J=8.0), 2.44 (s, 0.6H), 2.40 (s, 2.4H), 1.94 (m, 2H), 1.53 (s, 9H), 1.05 (t, 3H, J=8.0 Hz).

d) 7-{2-[1-(2-Ethoxycarbonyl-1-p-tolyl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-{2-[1-(2-ethoxycarbonyl-1-p-tolyl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure outlined in Example 18, step (e). Transesterification occurred during the reduction, resulting in a 1:4 mixture of ethyl and methyl esters, in a 60% yield. ¹H NMR (CDCl₃) δ 7.30 (d, 1H, J=7.2 Hz), 7.18 (m, 2H), 7.10 (m, 5H), 6.94 (d, 1H, J=7.6 Hz), 6.80 (dd, 1H, J=2.4, 7.6 Hz), 6.41 (d, 1H, J=3.2 Hz), 5.98 (t, 1H, J=7.6 Hz), 4.35 (t, 2H, J=6.8 Hz), 4.13 (m, 0.4H, ethyl ester), 3.77 (m, 2H), 3.59 (s, 2.4H, methyl ester), 3.27 (m, 2H), 3.20 (t, 2H, J=6.8 Hz), 2.72 (t, 2H, J=6.8 Hz), 2.31, (s, 3H), 1.95 (m, 2H), 1.49 (s, 9H), 1.11 (t, 0.6H, J=7.2 Hz, ethyl ester).

e) 3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-p-tolyl-propionic acid ethyl ester

The title compound was synthesized from 7-{2-[1-(2-Ethoxycarbonyl-1-p-tolyl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure outlined in Example 16, step (f), in a 78% yield of a 1:1 mixture of ethyl and methyl esters. ¹H NMR (CDCl₃) δ 7.16 (m, 3H), 7.08 (m, 5H), 6.81 (dd, 1H, J=2.4, 9.6 Hz), 6.48 (d, 1H, J=8.0 Hz), 6.43(d, 1H, J=4.0 Hz), 5.98 (t, 1H, J=8.0 Hz), 5.17 (s, 1H), 4.27 (t, 2H, J=8.0 Hz), 4.03 (m, 1H, ethyl ester), 3.59 (s, 1.5H, methyl ester) 3.39 (m, 2H), 3.25 (m, 2H), 3.07 (t, 2H, J=8.0 Hz), 2.69 (t, 2H, J=6.8 Hz), 2.29 (s, 3H), 1.90 (m, 2H), 1.10 (t, 1.5H, J=7.2 Hz, ethyl ester).

f) 3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-p-tolyl-propionic acid

The title compound was synthesized from 3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-p-tolyl-propionic acid ethyl ester using the procedure outlined in Example 18, step (g), in 23% yield. ¹H NMR (DMSO-d₆) δ 7.62 (d, 1H, J=3.2 Hz), 7.35 (d, 1H, J=9.0 Hz), 7.20 (d, 2H, J=8.1 Hz), 7.06 (m, 3H), 7.01(d, 1H, J=4.0 Hz), 6.69 (dd, 1H, J=2.4, 6.5 Hz), 6.36 (m, 3H), 5.89 (m, 1H), 4.19 (t, 2H, J=6.9 Hz), 3.25 (m, 4H) 2.86 (t, 2H, J=6.9 Hz), 2.60 (t, 2H, J=6.1 Hz), 2.21 (s, 3H), 1.74 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₈H₃₀N₃O₃: 456.2 (M+H); found 456.3.

EXAMPLE 20

3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-m-tolyl-propionic acid

a) 1-Ethynyl-3-methyl-benzene

The title compound was synthesized from commercially available 3-iodotoluene using the procedures outlined in Example 18, step (a) and step (b), in 37% yield overall. ¹H NMR (CDCl₃) δ 7.32 (m, 2H), 7.18 (m, 2H), 3.03 (s, 1H), 2.32 (s, 3H).

b) 3-Chloro-3-m-tolyl-acrylic acid ethyl ester

The title compound was synthesized from 1-ethynyl-3-methyl-benzene using the procedure outlined in Example 18, step (c), in 70% yield. ¹H NMR (CDCl₃) δ 7.48 (m, 2H), 7.29 (m, 2H), 6.53 (s, 1H), 4.27 (m, J=8.0 Hz), 2.39 (s, 3H), 1.34 (t, 3H, J=7.2 Hz).

c) 7-{2-[1-(2-Ethoxycarbonyl-1-m-tolyl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 3-chloro-3-m-tolyl-acrylic acid ethyl ester and 7-[2-(1H-indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure outlined in Example 18, step (d), in a 40% yield as an E/Z isomeric mixture. ¹H NMR (CDCl₃) δ 7.19 (m, 2.2H), 7.04 (m, 2.6H), 6.96 (m, 1.6H), 6.86 (m, 1.6H), 6.66 (m, 2H), 6.48 (d, 0.8H, J=0.8 Hz), 6.40 (d, 0.2H, J=0.8 Hz), 6.11 (s, 0.8H), 6.04 (s, 0.2H), 4.30 (m, 2H), 3.93 (m, 2H), 3.68 (m, 2H), 3.13 (t, 2H, J=6.4), 2.65 (t, 2H, J=6.4 Hz), 2.24 (s, 3H), 1.85 (m, 2H), 1.43 (s, 9H), 0.94 (t, 3H, J=6.8 Hz).

The title compound was synthesized from 7-{2-[1-(2-ethoxycarbonyl-1-m-tolyl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure outlined in Example 18, step (e). Transesterification occurred during the reduction, resulting in a 2:3 mixture of ethyl and methyl esters, in a 58% yield. ¹H NMR (CDCl₃) δ 7.31 (d, 1H, J=4.4 Hz), 7.21 (m, 3H), 7.05 (m, 5H), 6.83 (dd, 1H, J=2.4, 9.6 Hz), 6.48 (d, 1H, J=3.2 Hz), 5.99 (t, 1H, J=7.6 Hz), 4.37 (t, 2H, J=7.0 Hz), 4.05 (m, 0.8H, ethyl ester), 3.77 (m, 2H), 3.60 (s, 1.8H, methyl ester) 3.28 (m, 2H), 3.21 (t, 2H, J=6.8 Hz), 2.74 (t, 2H, J=6.8 Hz), 2.29 (s, 3H), 1.93 (m, 2H), 1.52 (s, 9H), 1.10 (t, 1.2H, J=7.2 Hz, ethyl ester).

e) 3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-m-tolyl-propionic acid ethyl ester

The title compound was synthesized from 7-{2-[1-(2-ethoxycarbonyl-1-p-tolyl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (f), in a 75% yield of a 1:1 mixture of ethyl and methyl ester. ¹H NMR (CDCl₃) δ 7.19 (m, 4H), 7.05 (m, 5H), 6.81 (dd, 1H, J=2.4, 9.6 Hz), 6.48 (d, 1H, J=8.0 Hz), 6.43(d, 1H, J=3.2 Hz), 5.97 (t, 1H, J=8.0 Hz), 5.30 (s, 1H), 4.29 (t, 2H, J=8.0 Hz), 4.05 (m, 1H, ethyl ester), 3.59 (s, 1.5H, methyl ester) 3.39 (t, 2H, J=8.0 Hz), 3.25 (m, 2H), 3.04 (t, 2H, J=8.0 Hz), 2.69 (t, 2H, J=6.8 Hz), 2.28 (s, 3H), 1.90 (m, 2H), 1.08 (t, 1.5H, J=8.0 Hz, ethyl ester).

f) 3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-m-tolyl-propionic acid

The title compound synthesized from 3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-m-tolyl-propionic acid ethyl ester using the procedure described in Example 18, step (g), in 37% yield. ¹H NMR (CDCl₃) δ 10.39 (s, 1H), 7.43 (d, 1H, J=4.0 Hz), 7.20 (d, 1H, J=8.0 Hz), 7.05 (m, 2H), 6.90 (m, 3H), 6.77(d, 1H, J=4.0 Hz), 6.54 (dd, 1H, J=4.0, 8.0 Hz), 6.40 (d, 1H, J=4.0 Hz), 6.19 (d, 1H, J=8.0 Hz), 6.02 (dd, 1H, J=4.0, 8.0 Hz), 3.25 (m, 6H) 2.45 (m, 4H), 2.19 (m, 3H), 1.76 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₈H₃₀N₃O₃: 456.2 (M+H); found 456.3.

EXAMPLE 21

3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-o-tolyl-propionic acid

a) 1-Ethynyl-2-methyl-benzene

The title compound was synthesized from commercially available 2-iodotoluene using the procedures outlined in Example 18, step (a) and step (b), in 45% yield overall. ¹H NMR (CDCl₃) δ 7.45 (m, 1H), 7.24 (m, 2H), 7.13 (m, 1H), 3.26 (s, 1H), 2.45 (s, 3H).

b) 3-Chloro-3-o-tolyl-acrylic acid ethyl ester

The title compound was synthesized from 1-ethynyl-2-methyl-benzene using the procedure outlined in Example 18, step (c), in 70% yield. ¹H NMR (CDCl₃) δ 7.25 (m, 5H), 6.17 (s, 1H), 4.27 (m, 2H), 2.40 (s, 3H), 1.34 (t, 3H, J=7.2 Hz).

c) 7-{2-[1-(2-Ethoxycarbonyl-1-o-tolyl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 3-chloro-3-o-tolyl-acrylic acid ethyl ester and 7-[2-(1H-indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure outlined in Example 18, step (d), in a 35% yield of an E/Z isomeric mixture. ¹H NMR (CDCl₃) δ 7.46 (m, 0.6H), 7.30 (m, 4.8H), 7.10 (m, 1.6H), 7.03 (d, 0.6H, J=3.6 Hz), 6.94 (m, 1H), 6.85 (m, 0.4H), 6.70 (m, 1H), 6.53 (d, 0.8H, J=2.8 Hz), 6.47 (d, 0.2H, J=2.8 Hz), 6.30 (s, 0.2H), 5.82 (s, 0.8H), 4.39 (m, 2H), 4.10 (m, 2H), 3.78 (m, 2H), 3.22 (t, 2H, J=6.8), 2.75 (t, 2H, J=6.8), 2.07 (s, 2.4H), 2.02 (s, 0.6H), 1.94 (m, 2H), 1.53 (s, 9H), 1.05 (m, 3H).

d) 7-{2-[1-(2-Ethoxycarbonyl-1-o-tolyl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-{2-[1-(2-ethoxycarbonyl-1-o-tolyl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 18, step (e). Transesterification occurred during the reduction, resulting in a 4:1 mixture of ethyl and methyl ester in a 72% yield. ¹H NMR (CDCl₃) δ 7.25 (m, 7H), 7.15 (d, 1H, J=2.4 Hz), 7.05 (m, 2H), 6.85 (dd, 1H, J=2.4, 9.6 Hz), 6.39 (d, 1H, J=3.2 Hz), 5.99 (t, 1H, J=7.6 Hz), 4.38 (t, 2H, J=7.0 Hz), 4.03 (m, 1.6H, ethyl ester), 3.77 (m, 2H), 3.60 (s, 0.6H, methyl ester), 3.21 (m, 4H), 2.74 (t, 2H, J=6.8 Hz), 2.40 (s, 3H), 1.93 (m, 2H), 1.53 (s, 9H), 1.10 (t, 2.4H, J=7.2 Hz, ethyl ester).

The title compound was synthesized from 7-{2-[1-(2-ethoxycarbonyl-1-o-tolyl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (f), in a 75% yield of a 1:1 mixture of ethyl and methyl esters. ¹H NMR (CDCl₃) δ 7.17 (m, 5H), 7.05 (m, 2H), 6.94 (d, 1H, J=3.2 Hz), 6.81 (dd, 1H, J=2.4, 6.4 Hz), 6.46 (d, 1H, J=7.6 Hz), 6.34 (d, 1H, J=3.2 Hz), 6.13 (t, 1H, J=7.2 Hz), 4.91 (s, 1H), 4.26 (t, 2H, J=6.8 Hz), 4.10 (m, 1H, ethyl ester), 3.56 (s, 1.5H, methyl ester), 3.38 (m, 2H), 3.17 (t, 2H, J=7.2 Hz), 3.08 (t, 2H, J=1.2 Hz), 2.67 (t, 2H, J=6.8 Hz), 2.20 (s, 3H), 1.88 (m, 2H), 1.07 (t, 1.5H, J=7.2 Hz).

f) 3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-o-tolyl-propionic acid

The title compound was synthesized from 3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-p-tolyl-propionic acid ethyl ester using the procedure outlined in Example 18, step (g), in 30% yield. ¹H NMR (DMSO-d₆) δ 7.49 (d, 1H, J=3.2 Hz), 7.33 (s, 1H), 7.14 (m, 5H), 7.05 (d, 1H, J=2.4 Hz), 6.69 (dd, 1H, J=2.4, 6.5 Hz), 6.55 (d, 1H, J=6.8 Hz), 6.38 (d, 1H, J=3.2 Hz), 6.06 (m, 1H), 4.21 (t, 2H, J=6.5 Hz), 3.21 (m, 4H) 2.99 (t, 2H, J=6.0 Hz), 2.67 (t, 2H, J=6.0 Hz), 2.34 (s, 3H), 1.77 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₈H₃₀N₃O₃: 456.2 (M+H); found 456.3.

EXAMPLE 22

3-Biphenyl-4-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

a) 3-Biphenyl-4-yl-3-chloro-acrylic acid ethyl ester

The title compound was synthesized from the commercially available 4-ethynyl-biphenyl using the procedure described in Example, 18 step (c), in 22% yield. ¹H NMR (Cl₃CD), δ: 7.76 (m, 2H), 7.61 (m, 5H), 7.45 (m, 2H), 6.60 (s, 1H), 4.29 (c, 2H, J=8.0 Hz), 1.34 (t, 3H, J=7.2 Hz).

b) 7-{2-[1-(1-Biphenyl-4-yl-2-ethoxycarbonyl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-[2-(1H-indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester and 3-biphenyl-4-yl-3-chloro-acrylic acid ethyl ester using the procedure described in Example 18, step (d), in an 8% yield as a mixture of E/Z isomers.
¹H NMR (Cl₃CD), δ: 7.65 (m, 2H), 7.59 (m, 3H), 7.46 (m, 4H), 7.37 (m, 0.35H), 7.32 (d, 1H, J=7.6 Hz), 7.12 (d, 0.65H, J=2.5 Hz), 7.09 (m, 1H), 6.99 (d, 0.35H, J=3.5 Hz), 6.95 (d, 0.65H, J=7.6 Hz), 6.94 (d, 0.35H, J=7.6 Hz), 6.80 (d, 0.65H, J=9.0 Hz), 6.78 (dd, 0.35H, J=2.5, 9.0 Hz), 6.72 (dd, 0.65H, J=2.3, 8.8 Hz), 6.59 (d, 0.65H, J=3.5 Hz), 6.51 (d, 0.35H, J=3.2 Hz), 6.27 (s, 0.65H), 6.16 (s, 0.35H), 4.38 (m, 2H), 4.12 (c, 1.3H, J=8.0 Hz), 4.00 (c, 0.7H, J=8.0 Hz), 3.76 (m, 2H), 2.73 (t, 2H, J=8 Hz), 3.21 (m, 2H), 1.92 (m, 2H), 1.52 (m, 9H), 1.28 (t, 1.95H, J=8.0 Hz), 1.18 (t, 1.05H, J=8.0 Hz).

c) 7-{2-[1-(1-Biphenyl-4-yl-2-ethoxycarbonyl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-{2-[1-(1-biphenyl-4-yl-2-ethoxycarbonyl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 18, step (e), in 59% yield. ¹H NMR (Cl₃CD), δ: 7.43 (m, 4H), 7.33 (m, 2H), 7.25 (m, 2H), 7.15 (m, 4H), 7.02 (d, 1H, J=2.3 Hz), 6.88 (d, 1H, J=7.4 Hz), 6.76 (dd, 1H, J=2.5, 9.0 Hz), 6.38 (d, 1H, J=3.2 Hz), 5.98 (t, 1H, J=7.4 Hz), 4.29 (m, 2H), 3.98 (c, 2H, J=7.2 Hz), 3.36 (m, 2H), 3.68 (m, 2H), 3.13 (m, 2H), 2.65 (m, 2H), 1.92 (m, 2H), 1.44 (s, 9H), 1.18 (t, 3H, J=7.2 Hz).

d) 3-Biphenyl-4-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester

The title compound was synthesized from 7-{2-[1-(1-biphenyl-4-yl-2-ethoxycarbonyl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (f), in 43% yield. ¹H NMR (Cl₃CD), δ: 7.44 (m, 4H), 7.34 (m, 3H), 7.26 (m, 1H), 7.16 (m, 4H), 7.02 (d, 1H, J=2.1 Hz), 6.73 (dd, 1H, J=2.3, 8.8 Hz), 6.41 (d, 1H, J=3.0 Hz), 5.98 (t, 1H, J=7.7 Hz), 4.23 (t, 2H, J=6.0 Hz), 3.98 (c, 2H, J=6.92 Hz), 3.68 (m, 2H), 3.37 (m, 2H), 3.05 (t, 2H, J=6.3 Hz), 2.64 (t, 2H, J=6.3 Hz), 1.83 (m, 2H), 1.03 (t, 3H, J=6.9 Hz).

e) 3-Biphenyl-4-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

The title compound was synthesized from 3-biphenyl-4-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester using the procedure described in Example 18, step (g), in 83% yield. ¹H NMR (Cl₃CD), δ: 7.45 (m, 3H), 7.39 (m, 2H), 7.32 (m, 2H), 7.24 (m, 2H), 7.15 (d, 1H, J=8.3 Hz), 7.02 (d, 1H, J=7.2 Hz), 6.80 (d, 1H, J=2.3 Hz), 6.57 (dd, 1H, J=2.3, 8.8 Hz), 6.44 (m, 1H), 6.19 (d, 1H, J=7.4 Hz), 6.11 (m, 1H), 3.54 (m, 2H), 3.32 (m, 2H), 3.18 (m, 2H), 2.55 (m, 4H), 1.76 (m, 2H). Mass Spectrum (LCMS, ESI) calculate for C₃₃H₃₂N₃O₃: 518.2, (M+1); found: 518.4.

EXAMPLE 23

3-(3,5-dichloro-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

a) (3,5-Dichloro-phenylethynyl)-trimethyl-silane

The title compound was synthesized from 1,3-dichloro-5-iodo-benzene using the procedure described in Example 18, step (a), in 98% yield. ¹H NMR (CDCl₃) δ 7.34-7.33 (m, 2H), 7.31-7.29 (m, 1H), 0.24 (s, 9H).

b) 1,3-Dichloro-5-ethynyl-benzene

To a solution of (3,5-dichloro-phenylethynyl)-trimethyl-silane (1.97 g, 8.1 mmol) in methanol (40 mL) was added a solution of potassium hydroxide (6.8 mg, 0.12 mmol) in H₂O (0.24 mL). After stirring at ambient temperature for 40 minutes, the reaction mixture was diluted with water (40 mL), and extracted with hexane until the extracting solvent showing no product by TLC. The combined organic layer was dried over MgSO₄, and concentrated to give the title compound (1.21 g, 87% yield) as a white solid. ¹H NMR (CDCl₃) δ 7.37-7.34 (m, 3H), 3.15 (s, 1H).

c) (3,5-Dichloro-phenyl)-propynoic acid ethyl ester

To a solution of diisopropylamine (0.23 mL) in THF (0.6 mL) at −78° C. was added a solution of n-butyllithium (0.46 mL, 2.0 M in hexane). The mixture was stirred at −78° C. for 20 minutes, 0° C. for 15 min., and cooled to −78° C. To this mixture was added a solution of 1,3-dichloro-5-ethynyl-benzene (136 mg, 0.80 mmol) in THF (1.0 mL) over 2 minutes. After stirring at −78° C. for 1 h, a solution of ethyl chloroformate (0.09 mL) in THF (0.2 mL) was added, and stirred for 1 h at −78° C. The reaction was quenched with saturated ammonium chloride, warmed up to ambient temperature, and extracted with ethyl acetate. The extract was dried over Na₂SO₄, concentrated, and flash chromatographed on silica gel, eluting with hexane to give the title compound (0.16 g, 87% yield) as a yellow oil. ¹H NMR (CDCl₃) δ 7.47-7.44 (m, 3H), 4.31 (q, 2H, J=7.2 Hz), 1.36 (t, 3H, J=7.2 Hz).

d) 7-(2-{1-[1-(3,5-Dichloro-phenyl)-2-ethoxycarbonyl-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from (3,5-dichloro-phenyl)-propynoic acid ethyl ester using the procedure described in Example 16, step (d1), in 43% yield as a mixture of E/Z isomers. ¹H NMR (CDCl₃) δ 7.49-7.43 (m, 1H), 7.32 (d, 1H, J=7.6 Hz), 7.27 (d, 1H, J=1.9 Hz), 7.18-7.15 (m, 1H), 7.10-7.00 (m, 1H), 6.96-6.93 (m, 1H), 6.85 (d, 1H, J=3.5 Hz), 6.83-6.76 (m, 1H), 6.59 (d, 0.2H, J=3.4 Hz), 6.52 (d, 0.8H, J=3.5 Hz), 6.20 (s, 0.2H), 6.19 (s, 0.8H), 4.41-4.36 (m, 2H), 4.12 (q, 2H, J=7.1 Hz), 3.76 (t, 2H, J=5.6 Hz), 3.21 (t, 2H, J=6.8 Hz), 2.74 (t, 2H, J=6.6 Hz), 1.96-1.90 (m, 2H), 1.52 (s, 9H), 1.26 (t, 3H, J=7.2 Hz).

e) 7-(2-{1-[1-(3,5-Dichloro-phenyl)-2-ethoxycarbonyl-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-(2-{1-[1-(3,5-dichloro-phenyl)-2-ethoxycarbonyl-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 18, step (e), in 75% yield. ¹H NMR (CDCl₃) δ 7.31 (d, 1H, J=7.6 Hz), 7.25-7.24 (m, 2H), 7.17-7.10 (m, 3H), 7.04-7.03 (m, 2H), 6.94 (d, 1H, J=7.6 Hz), 6.84 (dd, 1H, J=2.4, 8.9 Hz), 6.48 (d, 1H, J=3.2 Hz), 5.93 (t, 1H, J=7.5 Hz), 4.37 (t, 2H, J=6.9 Hz), 4.10-4.04 (m, 2H), 3.76 (t, 2H, J=6.0 Hz), 3.30-3.16 (m, 4H), 2.73 (t, 2H, J=6.6 Hz), 1.92 (p, 2H, J=6.6 Hz), 1.50 (s, 9H), 1.12 (t, 3H, J=7.1 Hz).

f) 3-(3,5-Dichloro-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

The title compound was synthesized from 7-(2-{1-[1-(3,5-dichloro-phenyl)-2-ethoxycarbonyl-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester in two steps using the procedures described in Example 16, step (f), and Example 18, step (e), in 41% yield. ¹H NMR (CDCl₃) δ 10.35 (bs, 1H), 7.44 (d, 1H, J=3.2 Hz), 7.19-7.11 (m, 3H), 7.04-7.02 (m, 2H), 6.87 (d, 1H, J=2.2 Hz), 6.63 (dd, 1H, J=2.2, 8.9 Hz), 6.49 (d, 1H, J=3.0 Hz), 6.29 (d, 1H, J=7.3 Hz), 6.05 (dd, 1H, J=4.8, 10.5 Hz), 3.73-3.67 (m, 1H), 3.56-3.51 (m, 1H), 3.48-3.37 (m, 2H), 3.26-3.08 (m, 2H), 2.70-2.53 (m, 4H), 1.85-1.82 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₇H₂₅Cl₂N₃O₃510.1 (M+H); found 510.4.

EXAMPLE 24

3-(3,5-Difluoro-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

a) (3,5-Difluoro-phenylethynyl)-trimethyl-silane

The title compound was synthesized from 1,3-difluoro-5-bromobenzene using the procedure described in Example 18, step (a), in 96% yield. ¹H NMR (CDCl₃) δ 7.02-6.93 (m, 2H), 6.80-6.74 (m, 1H), 0.25 (s, 9H).

b) 1-Ethynyl-3,5-difluoro-benzene

The title compound was synthesized from (3,5-difluoro-phenylethynyl)-trimethyl-silane using the procedure described in Example 23, step (b), in 79% yield. ¹H NMR (CDCl₃) δ 7.04-6.97 (m, 2H), 6.85-6.80 (m, 1H), 3.14 (s, 1H).

c) (3,5-Difluoro-phenyl)-propynoic acid ethyl ester

The title compound was synthesized from 1-ethynyl-3,5-difluoro-benzene using the procedure described in Example 23, step (c), in 69% yield. ¹H NMR (CDCl₃) δ 7.13-7.07 (m, 2H), 6.92 (tt, 1H, J=2.3, 8.8 Hz), 4.31 (q, 2H, J=7.2 Hz), 1.36 (t, 3H, J=7.2 Hz).

d) 7-(2-{1-[1-(3,5-Difluoro-phenyl)-2-ethoxycarbonyl-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from (3,5-difluoro-phenyl)-propynoic acid ethyl ester using the procedure described in Example 16, step (d1), in 47% yield, as a mixture of E/Z isomers. ¹H NMR (CDCl₃) δ 7.33-7.31 (m, 1H), 7.13-7.08 (m, 1H), 7.02 (d, 1H, J=3.3 Hz), 6.95-6.80 (m, 4H), 6.75-6.74 (m, 2H), 6.58 (d, 0.54H, J=3.4 Hz), 6.52 (dd, 0.46H, J=0.6, 3.5 Hz), 6.23 (s, 0.54H), 6.18 (s, 0.46H), 4.41-4.36 (m, 2H), 4.00 (q, 2H, J=7.1 Hz), 3.76 (t, 2H, J=6.0 Hz), 3.21 (t, 2H, J=6.9 Hz), 2.73 (t, 2H, J=6.6 Hz), 1.96-1.89 (m, 2H), 1.52 (s, 9H), 1.02 (t, 3H, J=7.1 Hz).

e) 7-(2-{1-[1-(3,5-Difluoro-phenyl)-2-methoxycarbonyl-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-(2-{1-[1-(3,5-difluoro-phenyl)-2-ethoxycarbonyl-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 18, step (e), in 71% yield. ¹H NMR (CDCl₃) δ 7.30 (d, 1H, J=7.6 Hz), 7.25-7.10 (m, 3H), 6.94-6.92 (m, 1H), 6.84-6.82 (m, 1H), 6.70-6.65 (m, 3H), 6.47 (d, 1H, J=3.3 Hz), 5.96 (t, 1H, J=7.9 Hz), 4.38-4.35 (m, 2H), 3.76-3.74 (m, 2H), 3.63 (s, 3H), 3.33-3.18 (m, 4H), 2.74-2.70 (m, 2H), 1.95-1.88 m, 2H), 1.51 (s, 9H).

f) 3-(3,5-Difluoro-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid methyl ester

The title compound was synthesized from 7-(2-{1-[1-(3,5-difluoro-phenyl)-2-ethoxycarbonyl-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (f), in 95% yield. Mass Spectrum (LCMS, ESI) calculated for C₂₈H₂₈F₂N₃O₃492.2 (M+H); found 492.4.

g) 3-(3,5-Difluoro-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

The title compound was synthesized from 3-(3,5-difluoro-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester using the procedure described in Example 18, step (g), in 80% yield. ¹H NMR (DMSO-d₆) δ 7.72 (d, 1H, J=3.3 Hz), 7.48 (d, 1H, J=8.9 Hz), 7.21 (d, 1H, J=7.0 Hz), 7.12-7.08 (m, 3H), 7.04 (d, 1H, J=2.3 Hz), 6.72 (dd, 1H, J=2.4, 8.9 Hz), 6.46 (d, 1H, J=7.5 Hz), 6.41 (d, 1H, J=3.3 Hz), 6.00 (dd, 1H, J=5.6, 9.3 Hz), 4.21 (t, 2H, J=6.8 Hz), 3.50-3.28 (m, 4H), 2.94 (t, 2H, J=6.4 Hz), 2.64 (t, 2H, J=5.9 Hz), 1.76 (p, 2H, J=5.8 Hz). Mass Spectrum (LCMS, ESI) calculated for C₂₇H₂₆F₂N₃O₃478.2 (M+H); found 478.3.

EXAMPLE 25

3-(3-cyano-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

a) 3-Trimethylsilanylethynyl-benzonitrile

The title compound was synthesized from 3-bromobenzonitrile using the procedure described in Example 18, step (a), in 99% yield. ¹H NMR (CDCl₃) δ7.74 (dt, 1H, J=0.6, 6.3 Hz), 7.68-7.65 (m, 1H), 7.60-7.65 (m, 1H), 7.44-7.40 (m, 1H), 0.26 (s, 9H).

b) 3-Ethynyl-benzonitrile

The title compound was synthesized from 3-trimethylsilanylethynyl-benzonitrile using the procedure described in Example 23, step (b), in 90% yield. ¹H NMR (CDCl₃) *7.77 (t, 1H, J=1.4 Hz), 7.70 (td, 1H, J=1.3, 7.8 Hz), 7.63 (td, 1H, J=1.4, 7.8 Hz), 7.45 (dt, 1H, J=0.4, 7.9 Hz), 3.19 (s, 1H).

c) (3-Cyano-phenyl)-propynoic acid ethyl ester

The title compound was synthesized from 3-ethynyl-benzonitrile using the procedure described in Example 23, step (c), in 80% yield. ¹H NMR (CDCl₃) *6.87-6.86 (m, 1H), 7.80 (td, 1H, J=1.3, 7.9 Hz), 7.73 (td, 1H, J=1.3, 7.9 Hz), 7.53 (t, 1H, J=7.9 Hz), 4.32 (q, 2H, J=7.2 Hz), 1.37 (t, 3H, J=7.2 Hz).

d) 7-(2-{1-[1-(3-Cyano-phenyl)-2-ethoxycarbonyl-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from (3-cyanophenyl)propynoic acid ethyl ester using the procedure described in Example 16, step (c1), in 71% yield as a mixture of E/Z isomers. ¹H NMR (CDCl₃) *7.79-7.48 (m, 4H), 7.33-7.31 (m, 1H), 7.13-7.10 (m, 1H), 7.06-7.02 (m, 1H), 6.94 (dd, 1H, J=2.3, 7.6 Hz), 6.84-6.79 (m, 1H), 6.75-6.66 (m, 1H), 6.60 (dd, 0.56H, J=0.6, 3.3 Hz), 6.53 (dd, 0.44H, J=0.5, 3.5 Hz), 6.23 (s, 0.6H), 6.22 (s, 0.4H), 4.41-4.36 (m, 2H), 4.11 (q, 0.9H, J=7.1 Hz), 4.03 (q, 1.1H, J=7.1 Hz), 3.78-3.75 (m, 2H), 3.21 (t, 2H, J=6.9 Hz), 2.73 (t, 2H, J=6.7 Hz), 1.92 (p, 2H, J=6.6 Hz), 1.52 (s, 9H), 1.20 (t, 1.3H, J=7.1 Hz), 1.04 (t, 1.7H, J=7.2 Hz).

e) 7-(2-{1-[1-(3-Cyano-phenyl)-2-ethoxycarbonyl-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-(2-{1-[1-(3-cyano-phenyl)-2-ethoxycarbonyl-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 18, step (e), in 80% yield as a mixture of ethyl and methyl esters. ¹H NMR (CDCl₃) *7.56-7.53 (m, 1H), 7.45-7.30 (m, 4H), 7.18-7.17 (m, 1H), 7.11-7.08 (m, 2H), 6.94 (d, 1H, J=7.6 Hz), 6.82 (dd, 1H, J=2.4, 8.9 Hz), 6.49 (d, 1H, J=3.2 Hz), 6.02 (t, 1H, J=7.5 Hz), 4.36 (t, 2H, J=6.9 Hz), 4.08 (q, 0.52H, J=7.1 Hz), 3.77-3.74 (m, 2H), 3.65 (s, 2.2H), 3.36-3.18 (m, 4H), 2.73 (t, 2H, J=6.6 Hz), 1.95-1.87 (m, 2H), 1.52 (s, 9H), 1.12 (t, 0.8H, J=7.1 Hz).

f) 3-(3-Cyano-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester

The title compound was synthesized from 3-(3-cyano-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester using the procedure described in Example 16, step (e), in 50% yield as a white solid. ¹H NMR (CDCl₃) *7.56-7.53 (m, 1H), 7.45-7.34 (m, 3H), 7.17 (t, 1H, J=3.0 Hz), 7.10-7.08 (m, 3H), 6.82 (dd, 1H, J=2.4, 8.9 Hz), 6.49-6.46 (m, 2H), 6.02 (t, 1H, J=7.5 Hz), 5.04 (bs, 1H), 4.28 (t, 2H, J=6.8 Hz), 4.07 (q, 0.6H, J=6.2 Hz), 3.63 (s, 2.1H), 3.42-3.39 (m, 2H), 3.35-3.22 (m, 4H), 3.03 (t, 2H, J=6.9 Hz), 2.69 (t, 2H, J=6.3 Hz), 1.93-1.87 (m, 2H), 1.12 (t, 0.9H, J=7.1 Hz). Mass Spectrum (LCMS, ESI) calculated for C₂₉H₂₉N₄O₃481.2 (methyl ester, M+H); found 481.4. Calculated for C₃₀H₃₁N₄O₃495.2 (ethyl ester, M+H); found 495.3.
The title compound was synthesized from 7-(2-{1-[1-(3-cyano-phenyl)-2-ethoxycarbonyl-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 18, step (g), in 50% yield as a pale yellow solid. ¹H NMR (CDCl₃) *9.72 (bs, 1H), 7.47 (d, 1H, J=6.5 Hz), 7.38-7.30 (m, 4H), 7.19 (d, 1H, J=7.3 Hz), 7.11 (d, 1H, J=8.9 Hz), 6.89 (bs, 1H), 6.64 (d, 1H, J=8.7 Hz), 6.48 (d, 1H, J=2.5 Hz), 6.35 (d, 1H, J=7.3 Hz), 6.09 (dd, 1H, J=5.5, 9.5 Hz), 3.85-3.68 (m, 2H), 3.38-3.35 (m, 2H), 3.29-3.13 (m, 2H), 2.79-2.83 (m, 4H), 1.87-1.81 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₈H₂₈N₄O₃467.2 (M+H); found 467.3.

EXAMPLE 26

3-(4-cyano-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

a) 4-Trimethylsilanylethynyl-benzonitrile

The title compound was synthesized from 4-benzobenzonitrile using the procedure described in Example 18, step (a), in 80% yield. ¹H NMR (CDCl₃) *7.60-7.58 (m, 2H), 7.54-7.52 (m, 2H), 0.26 (s, 9H).

b) 4-Ethynyl-benzonitrile

The title compound was synthesized from 4-trimethylsilanylethynyl-benzonitrile using the procedure described in Example 23, step (b), in 75% yield. ¹H NMR (CDCl₃) *7.64-7.61 (m, 2H), 7.59-7.56 (m, 2H), 3.30 (s, 1H).

c) (4-Cyano-phenyl)-propynoic acid ethyl ester

The title compound was synthesized from 4-ethynyl-benzonitrile using the procedure described in Example 23, step (c), in 59% yield. The crude product was used in the next reaction without further purification.

d) 7-(2-{1-[1-(4-Cyano-phenyl)-2-ethoxycarbonyl-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from (4-cyano-phenyl)-propynoic acid ethyl ester using the procedure described in Example 16, step (c1), in 78% yield as a mixture of E/Z isomers. ¹H NMR (CDCl₃) *7.74-7.64 (m, 2H), 7.51 (d, 1H, J=8.3 Hz), 7.39-7.37 (m, 1H), 7.32 (d, 1H, J=7.6 Hz), 7.12-7.09 (m, 1H), 7.05-7.03 (m, 1H), 6.94 (d, 1H, J=7.6 Hz), 6.84-6.65 (m, 2H), 6.59 (d, 0.6H, J=3.3 Hz), 6.52 (d, 0.4H, J=3.5 Hz), 6.27 (s, 0.6H), 6.23 (s, 0.4H), 4.40-4.35 (m, 2H), 4.11 (q, 0.8H, J=7.1 Hz), 4.03 (q, 1.2H, J=7.1 Hz), 3.76 (t, 2H, J=6.0 Hz), 3.20 (t, 2H, J=6.9 Hz), 2.73 (t, 2H, J=6.7 Hz), 1.93 (p, 2H, J=6.6 Hz), 1.52 (s, 9H), 1.20 (t, 1.2H, J=7.1 Hz), 1.04 (t, 1.8H, J=7.1 Hz).

e) 3-(4-Cyano-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid methyl ester

The title compound was synthesized from 7-(2-{1-[1-(4-cyano-phenyl)-2-ethoxycarbonyl-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 18, step (e), in 45% yield. ¹H NMR (CDCl₃) *7.58-7.55 (m, 2H), 7.37 (d, 1H, J=3.2 Hz), 7.32-7.29 (m, 1H), 7.26-7.11 (m, 2H), 7.03 (d, 1H, J=2.3 Hz), 6.73-6.69 (m, 1H), 6.46-6.43 (m, 2H), 6.09-6.05 (m, 1H), 4.19 (t, 2H, J=6.8 Hz), 3.56 (s, 3H), 3.41-3.33 (m, 4H), 2.94 (t, 2H, J=7.0 Hz), 2.66 (t, 2H, J=6.2 Hz), 1.83 (p, 2H, J=6.3 Hz). Mass Spectrum (LCMS, ESI) calculated for C₂₉H₂₉N₄O₃481.2 (M+H); found 481.4.

f) 3-(4-Cyano-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

The title compound was synthesized from 3-(4-cyano-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid methyl ester using the procedure described in Example 18, step (g), in 43% yield. ¹H NMR (CDCl₃) *10.38 (s, 1H), 7.52-7.44 (m, 3H), 7.20-7.10 (m, 4H), 6.87 (s, 1H, J=2.3 Hz), 6.62 (dd, 1H, J=2.3, 8.9 Hz), 6.48 (d, 1H, J=3.1 Hz), 6.30 (d, 1H, J=7.3 Hz), 6.14 (dd, 1H, J=5.1, 10.2 Hz), 3.75-3.70 (m, 1H), 3.60-3.55 (m, 1H), 3.39 (bt, 2H, J=5.1 Hz), 3.28-3.12 (m, 2H), 2.74-2.58 (m, 4H), 1.87-1.82 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₈H₂₇N₄O₃467.2 (M+H); found 467.3.

EXAMPLE 27

3-(2-Methoxy-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

a) (2-Methoxy-phenylethynyl)-trimethyl-silane

The title compound was synthesized from commercially available 1-iodo-2-methoxy-benzene using the procedure described in Example 18, step (a), in 98% yield. ¹H NMR (CDCl₃) δ 7.60 (dd, 1H, J=1.8, 5.8 Hz), 7.43 (m, 1H), 7.04 (m, 2H), 4.03 (s, 3H), 0.34 (s, 9H).

b) 2-Methoxy-phenylethynyl

The title compound was synthesized from (2-methoxy-phenylethynyl)-trimethyl-silane using the procedure described in Example 18, step (b), in 63% yield. ¹H NMR (CDCl₃) δ 7.46 (dd, 1H, J=1.6, 6.0 Hz), 7.30 (m, 1H), 6.90 (m, 2H), 3.89 (s, 3H), 3.30 (s, 1H).

c) (2-Methoxy-phenyl)-propynoic acid ethyl ester

The title compound was synthesized from 2-methoxy-phenylethynyl using the procedure described in Example 23, step (c), in 71% yield. ¹H NMR (CDCl₃) δ 7.27 (m, 1H), 7.16 (m, 1H), 7.09 (m, 1H), 6.98 (m, 1H), 4.28 (q, 2H, J=7.2 Hz), 3.79 (s, 3H), 1.25 (t, 3H, J=7.2 Hz).

d) 7-(2-{1-[2-Ethoxycarbonyl-1-(2-methoxy-phenyl)-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from (2-methoxy-phenyl)-propynoic acid ethyl ester and 7-[2-(1H-Indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (d1), in a 80% yield as an E/Z isomeric mixture. ¹H NMR (CDCl₃) [E/Z mixture] δ 7.45 (m, 1H), 7.28-6.95 (m, 4H), 6.85-6.60 (m, 4H), 6.49 (m, 2H), 6.25 (s, 1H), 4.39 (m, 2H), 4.09 (q, 2H, J=7.2 Hz), 3.68 (s, 2H), 3.58 (s, 1H), 3.80 (m, 2H), 3.20 (m, 2H), 2.70 (m, 2H), 1.89 (m, 2H), 1.50 (s, 9H), 1.25 (t, 3H, J=6.8 Hz). Mass Spectrum (LCMS, ESI) calculated for C₃₀H₃₂N₃O₄498.2 (M-Boc+H); found 498.4.

e) 7-(2-{1-[2-Ethoxycarbonyl-1-(2-methoxy-phenyl)-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-(2-{1-[2-ethoxycarbonyl-1-(2-methoxy-phenyl)-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 18, step (e), in 44% yield. ¹H NMR (CDCl₃) δ 7.34-7.21 (m, 4H), 7.14 (m, 2H), 6.99-6.80 (m, 4H), 6.45 (m, 1H), 6.36 (m, 1H), 4.37 (m, 2H), 4.04 (q, 2H, J=7.1 Hz), 3.87 (s, 3H), 3.78 (m, 2H), 3.23 (m, 4H), 2.74 (m, 2H), 1.94 (m, 2H), 1.54 (s, 9H), 1.08 (t, 3H, J=7.1 Hz).

f) 3-(2-Methoxy-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester

The title compound was synthesized from 7-(2-{1-[2-ethoxycarbonyl-1-(2-methoxy-phenyl)-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (f), in 84% yield. ¹H NMR (CDCl₃) δ 7.25-7.18 (m, 3H), 7.10 (m, 2H), 6.90-6.81 (m, 4H), 6.44 (m, 2H), 6.38 (m, 1H), 5.10 (bs, 1H), 4.25 (m, 2H), 4.11 (q, 2H, J=7.2 Hz), 3.84 (s, 3H), 3.41 (m, 2H), 3.24 (m, 2H), 3.22 (m, 2H), 2.65 (m, 2H), 1.89 (m, 2H), 1.21 (t, 3H, J=7.2 Hz).

g) 3-(2-Methoxy-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

The title compound was synthesized from 3-(2-methoxy-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester using the procedure described in Example 18, step (g), in 14% yield. ¹H NMR (CDCl₃/CD₃OD) δ 7.57 (m, 1H), 7.27-7.14 (m, 3H), 6.90 (d, 1H, J=8.4 Hz), 6.80 (m, 3H), 6.60 (dd, 1H, J=2.4, 6.4 Hz), 6.46 (m, 2H), 6.29 (d, 1H, J=7.6 Hz), 3.93 (s, 3H), 3.54 (m, 2H), 3.45 (m, 2H), 3.36-3.12 (m, 4H), 2.66 (m, 2H), 1.86 (m, 2H); Mass Spectrum (LCMS, ESI) calculated for C₂₈H₃₀N₃O₄472.2 (M+H); found 472.3.

EXAMPLE 28

3-(3-Methoxy-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

a) (3-Methoxy-phenylethynyl)-trimethyl-silane

The title compound was synthesized from commercially available 1-iodo-3-methoxy-benzene using the procedure described in Example 18, step (a), in 98% yield. ¹H NMR (CDCl₃) δ 7.23 (t, 1H, J=7.8 Hz), 7.08 (dt, 1H, J=1.2, 7.6 Hz), 7.02 (m, 1H), 6.89 (m, 1H), 3.82 (s, 3H), 0.28 (s, 9H).

b) 3-Methoxy-phenylethynyl

The title compound was synthesized from (3-methoxy-phenylethynyl)-trimethyl-silane using the procedure described in Example 18, step (b), in 74% yield. ¹H NMR (CDCl₃) δ 7.25 (t, 1H, J=7.9 Hz), 7.12 (d, 1H, J=1.0 Hz), 7.04 (m, 1H), 6.93 (m, 1H), 3.82 (s, 3H), 3.09 (s, 1H).

c) (3-Methoxy-phenyl)-propynoic acid ethyl ester

The title compound was synthesized from 3-methoxy-phenylethynyl using the procedure described in Example 23, step (c), in 87% yield. ¹H NMR (CDCl₃) δ 7.26 (t, 1H, J=8.0 Hz), 7.18 (m, 1H), 7.08 (m, 1H), 6.98 (m, 1H), 4.30 (q, 2H, J=7.2 Hz), 3.79 (s, 3H), 1.35 (t, 3H, J=7.2 Hz).

d) 7-(2-{1-[2-Ethoxycarbonyl-1-(3-methoxy-phenyl)-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from (3-methoxy-phenyl)-propynoic acid ethyl ester and 7-[2-(1H-Indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (d1), in a 90% yield as an E/Z isomeric mixture. ¹H NMR (CDCl₃) [E/Z mixture] δ 7.45-6.68 (m, 9H), 6.49 (m, 2H), 6.23 (s, 1H), 4.37 (m, 2H), 4.13 (q, 2H, J=7.2 Hz), 3.76 (t, 2H, J=5.2 Hz), 3.68 (s, 2.1H), 3.58 (s, 0.9H), 3.20 (m, 2H), 2.73 (t, 2H, J=6.8 Hz), 1.90 (m, 2H), 1.51 (s, 9H), 1.26 (t, 2.1H, J=7.2 Hz), 1.13 (t, 0.9H, J=7.2 Hz). Mass Spectrum (LCMS, ESI) calculated for C₃₀H₃₂N₃O₄498.2 (M-Boc+1); found 498.4.

e) 7-(2-{1-[2-Ethoxycarbonyl-1-(3-methoxy-phenyl)-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-(2-{1-[2-ethoxycarbonyl-1-(3-methoxy-phenyl)-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 18, step (e), in 38% yield. ¹H NMR (CDCl₃) δ 7.32 (d, 1H, J=7.6 Hz), 7.22 (m, 3H), 7.10 (d, 1H, J=2.4 Hz), 6.96 (d, 1H, J=7.6 Hz), 6.80 (m, 3H), 6.71 (m, 1H), 6.45 (d, 1H, J=3.2 Hz), 5.99 (t, 1H, J=7.5 Hz), 4.38 (t, 2H, J=9.6 Hz), 4.07 (q, 2H, J=7.2 Hz), 3.77 (m, 2H), 3.74 (s, 3H), 3.33-3.20 (m, 4H), 2.75 (t, 2H, J=6.6 Hz), 1.93 (m, 2H), 1.53 (s, 9H), 1.12 (t, 3H, J=7.1 Hz). Mass Spectrum (LCMS, ESI) calculated for C₃₀H₃₄N₃O₄500.3 (M-Boc+1); found 500.4.

f) 3-(3-Methoxy-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester

The title compound was synthesized from 7-(2-{1-[2-ethoxycarbonyl-1-(3-methoxy-phenyl)-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (f), in 78% yield. ¹H NMR (CDCl₃) δ 7.21 (m, 3H), 7.05 (m, 2H), 6.80 (m, 4H), 6.45 (m, 2H), 6.00 (t, 1H, J=8.0 Hz), 4.86 (bs, 1H), 4.30 (t, 2H, J=8.0 Hz), 4.05 (q, 2H, J=8.0 Hz), 3.80 (s, 3H), 3.43 (m, 2H), 3.27 (m, 2H), 3.08 (m, 2H), 2.72 (m, 2H), 1.94 (m, 2H), 1.10 (t, 3H, J=8.0 Hz).

g) 3-(3-Methoxy-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

The title compound was synthesized from 3-(3-methoxy-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester using the procedure described in Example 18, step (g), in 22% yield. ¹H NMR (CDCl₃) δ 10.5 (bs, 1H), 7.50 (d, 1H, J=3.2 Hz), 7.26 (m, 1H), 7.15 (m, 1H), 7.07 (m, 1H), 6.82 (d, 1H, J=2.3 Hz), 6.72 (m, 3H), 6.60 (dd, 1H, J=2.3, 6.5 Hz), 6.46 (dd, 1H, J=3.0 Hz), 6.24 (d, 1H, J=7.3 Hz), 6.09 (m, 1H), 3.70 (s, 3H), 3.57 (m, 1H), 3.46-3.15 (m, 6H), 2.59 (m, 3H), 2.446 (m, 1H), 1.81 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₈H₃₀N₃O₄472.2 (M+H); found 472.3.

EXAMPLE 29

3-(4-Methoxy-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

a) (4-Methoxy-phenylethynyl)-trimethyl-silane

The title compound was synthesized from commercially available 1-iodo-4-methoxy-benzene using the procedure described in Example 18, step (a), in 95% yield. ¹H NMR (CDCl₃) δ 7.43 (d, 2H, J=4.6 Hz), 6.83 (d, 2H, J=4.6 Hz), 3.82 (s, 3H), 0.26 (s, 9H).

b) 4-Methoxy-phenylethynyl

The title compound was synthesized from (4-methoxy-phenylethynyl)-trimethyl-silane using the procedure described in Example 18, step (b), in 88% yield. ¹H NMR (CDCl₃) δ 7.46 (d, 2H, J=4.9 Hz), 6.87 (d, 2H, J=4.9 Hz), 3.83 (s, 3H), 3.02 (s, 1H).

c) (4-Methoxy-phenyl)-propynoic acid ethyl ester

The title compound was synthesized from 4-methoxy-phenylethynyl using the procedure described in Example 23, step (c), in 69% yield. ¹H NMR (CDCl₃) δ 7.53 (d, 2H, J=8.8 Hz), 6.87 (d, 2H, J=8.8 Hz), 4.28 (q, 2H, J=7.2 Hz), 3.82 (s, 3H), 1.34 (t, 3H, J=7.2 Hz).

d) 7-(2-{1-[2-Ethoxycarbonyl-1-(4-methoxy-phenyl)-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from (4-methoxy-phenyl)-propynoic acid ethyl ester and 7-[2-(1H-Indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (d1), in a 88% yield as an E/Z isomeric mixture. ¹H NMR (CDCl₃) [E/Z mixture] δ 7.32 (m, 2H), 7.21 (m, 1H), 7.11-6.85 (m, 6H), 6.70 (m, 1H), 6.50 (m, 1H), 6.13 (s, 0.5H), 6.03 (s, 0.5H), 4.40 (m, 2H), 4.10 (q, 2H, J=7.2 Hz), 3.86 (s, 1.5H), 3.83 (s, 1H), 3.76 (t, 2H, J=5.2 Hz), 3.70 (t, 2H, J=6.0 Hz), 3.20 (t, 2H, J=6.8 Hz), 2.70 (t, 2H, J=6.8 Hz, J=6.8 Hz), 1.90 (m, 2H), 1.49 (s, 9H), 1.23 (t, 1.5H, J=7.2 Hz), 1.18 (t, 1.5H, J=7.2 Hz). Mass Spectrum (LCMS, ESI) calculated for C₃₀H₃₂N₃O₄498.2 (M-Boc+H); found 498.4.

e) 7-(2-{1-[2-Ethoxycarbonyl-1-(4-methoxy-phenyl)-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-(2-{1-[2-ethoxycarbonyl-1-(4-methoxy-phenyl)-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 18, step (e), in 37% yield. ¹H NMR (CDCl₃) δ 7.24-7.00 (m, 6H), 6.87-6.73 (m, 4H), 6.33 (d, 1H, J=2.8 Hz), 5.89 (m, 1H), 4.28 (t, 2H, J=6.8 Hz), 3.96 (q, 2H, J=7.2 Hz), 3.68 (m, 5H), 3.16 (m, 4H), 2.73 (m, 2H), 1.85 (m, 2H), 1.44 (s, 9H), 1.02 (t, 3H, J=7.2 Hz).

f) 3-(4-Methoxy-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester

The title compound was synthesized from 7-(2-{1-[2-ethoxycarbonyl-1-(4-methoxy-phenyl)-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (f), in 80% yield. ¹H NMR (CDCl₃) δ 7.28-7.08 (m, 6H), 6.83 (m, 4H), 6.43 (d, 1H, J=2.8 Hz), 5.97 (m, 1H), 4.89 (bs, 1H), 4.28 (m, 2H), 4.05 (q, 2H, J=7.2 Hz), 3.77 (s, 3H), 3.41 (m, 2H), 3.26 (m, 2H), 3.04 (m, 2H), 2.66 (m, 2H), 1.91 (m, 2H), 1.11 (t, 3H, J=7.2 Hz).

g) 3-(4-Methoxy-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

The title compound was synthesized from 3-(4-methoxy-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester using the procedure described in Example 18, step (g), in 15% yield. ¹H NMR (CDCl₃) δ 10.6 (bs, 1H), 7.47 (d, 1H, J=3.2 Hz), 7.28 (d, 1H, J=8.9 Hz), 7.08 (m, 3H), 6.84 (d, 1H, J=2.3 Hz), 6.76 (d, 2H, J=8.8 Hz), 6.61 (dd, 1H, J=2.4, 6.5 Hz), 6.46 (m, 1H), 6.25 (d, 1H, J=7.3 Hz), 6.08 (m, 1H), 3.73 (s, 3H), 3.59 (m, 1H), 3.40 (m, 3H), 3.29-3.13 (m, 2H), 2.60 (m, 4H), 2.46 (m, 1H), 1.85 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₈H₃₀N₃O₄472.2 (M-Boc+H); found 472.3.

EXAMPLE 30

3-Quinolin-3-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

a) 3-Ethynyl-quinoline

The title compound was synthesized from 3-bromoquinoline using the procedures described in Example 18, step (a) and step (b), in 68% yield. ¹H NMR Cl₃CD, δ: 3.28 (s, 1H), 7.60 (m, 1H), 7.74 (m, 1H), 7.80 (m, 1H), 8.09 (d, 1H, J=8.8 Hz), 8.29 (d, 1H, J=2.0 Hz), 8.95 (d, 1H, J=2.0 Hz).

b) Quinolin-3-yl-propynoic acid ethyl ester

The title compound was synthesized from 3-ethynyl-quinoline using the procedure described in Example 23, step (c), in 34% yield. ¹H NMR Cl₃CD, δ: 1.38 (t, 3H, J=7.2 Hz), 4.34 (c, 2H, J=7.2 Hz), 7.60 (m, 1H), 7.80 (m, 2H), 8.11 (d, 1H, J=8.4 Hz), 8.40 (d, 1H, J=2.0 Hz), 8.99 (d, 1H, J=2.0 Hz).

c) 7-{2-[1-2-Ethoxycarbonyl-1-quinolin-3-yl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from quinolin-3-yl-propynoic acid ethyl ester and 7-[2-(1H-indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (d1), in 81% yield as an E/Z isomeric mixture. ¹H NMR (CDCl₃) δ 8.90 (m, 1H), 8.14 (m, 1.3H), 8.04 (m, 0.7H), 7.79 (m, 2H), 7.58 (m, 1H), 7.30 (d, 1H, J=7.6 Hz), 7.13(m, 2H), 6.93 (m, 1.3H), 6.75 (m, 1.7H), 6.63 (d, 0.7H, J=3.2 Hz), 6.53 (d, 0.3H, J=3.2 Hz), 6.38 (s, 0.7H), 6.32 (s, 0.3H), 4.38 (m, 2H), 4.05 (m, 2H), 3.75 (t, 2H, J=6.4 Hz), 3.19 (t, 2H, J=6.4 Hz), 2.72 (t, 2H, J=6.4 Hz), 1.91 (m, 2H), 1.51 (s, 9H), 1.13 (t, 0.9H, J=7.0 Hz), 1.05 (t, 2.1H, J=7.0 Hz).

d) 7-{2-[1-(2-Ethoxycarbonyl-1-quinolin-3-yl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-{2-[1-2-ethoxycarbonyl-1-quinolin-3-yl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 18, step (e), in 17% yield. ¹H NMR (CDCl₃) δ 8.81 (d, 1H, J=4.4 Hz), 8.07 (d, 1H, J=2.1 Hz), 7.88 (d, 1H, J=2.4 Hz), 7.10 (m, 2H), 7.54 (m, 1H), 7.25 (m, 3H), 7.11 (d, 1H, J=2.0 Hz), 6.94 (d, 1H, J=7.6 Hz), 6.82 (dd, 1H, J=2.4, 6.4 Hz), 6.50 (d, 1H, J=3.2 Hz), 6.24 (t, 1H, J=7.6 Hz), 4.37 (t, 2H J=6.8 Hz), 4.10 (m, 2H), 3.76 (m, 2H), 3.40 (t, 2H, J=7.6 Hz), 3.20 (t, 2H, J=6.8 Hz), 2.73 (t, 2H, J=6.8 Hz), 1.92 (m, 2H), 1.51 (s, 9H), 1.13 (t, 3H, J=6.8 Hz).

e) 3-Quinolin-3-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester

The title compound was synthesized from 7-{2-[1-(2-ethoxycarbonyl-1-quinolin-3-yl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (f), in 60% yield. ¹H NMR (CDCl₃) δ 8.83 (d, 1H, J=2.0 Hz), 8.08 (d, 1H, J=8.8 Hz), 7.88 (d, 1H, J=2.0 Hz), 7.71 (m, 2H), 7.54 (m, 1H), 7.23 (m, 2H), 7.10 (m, 2H), 6.84 (dd, 1H, J=2.4, 6.4 Hz), 6.49 (m, 2H), 6.24 (t, 1H, J=3.2 Hz), 4.95 (s, 1H), 4.29 (t, 2H, J=6.8 Hz), 4.08 (m, 2H), 3.41 (m, 4H), 3.05 (t, 2H, J=6.4 Hz), 2.70 (t, 2H, J=6.0 Hz), 1.92 (m, 2H), 1.13 (t, 3H, J=7.2 Hz).

f) 3-Quinolin-3-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

The title compound was synthesized from 3-quinolin-3-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester using the procedure described in Example 18, step (g), in 21% yield. ¹H NMR (CDCl₃) δ 10.21 (bs, 1H), 8.87 (d, 1H, J=2.4 Hz), 8.04 (d, 1H, J=8.8 Hz), 7.78 (d, 1H, J=1.6 Hz), 7.67 (m, 2H), 7.50 (m, 2H), 7.13 (d, 2H, J=7.6 Hz), 6.91 (d, 1H, J=2.4 Hz), 6.54 (dd, 1H, J=0.8, 8.8 Hz), 6.52 (d, 1H, J=2.8 Hz), 6.30 (m, 2H), 3.71 (m, 2H), 3.37 (m, 4H), 2.65 (m, 4H), 1.82 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₃₀H₂₉N₄O₃: 493.2 (M+H), found: 493.3.

EXAMPLE 31

3-(3-Chloro-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

a) (3-Chloro-phenylethynyl)-trimethyl-silane

The title compound was synthesized from commercially available 1-iodo-3-chloro-benzene using the procedure described in Example 18, step (a), in 98% yield. ¹H NMR (CDCl₃) δ 7.48 (t, 1H, J=1.8 Hz), 7.36 (dt, 1H, J=1.4, 7.5 Hz), 7.30 (m, 1H), 7.26 (m, 1H), 0.28 (s, 9H).

b) 3-Chloro-phenylethynyl

The title compound was synthesized from (3-chloro-phenylethynyl)-trimethyl-silane using the procedure described in Example 23, step (b), in 98% yield. ¹H NMR (CDCl₃) δ 7.48 (m, 1H), 7.38 (m, 2H), 7.25 (m, 1H), 3.11 (s, 1H).

c) (3-Chloro-phenyl)-propynoic acid ethyl ester

The title compound was synthesized from 4-chloro-phenylethynyl using the procedure described in Example 23, step (c), in 52% yield. ¹H NMR (CDCl₃) δ 7.61 (m, 1H), 7.41 (m, 2H), 7.25 (m, 1H), 4.32 (q, 2H), 1.31 (t, 3H).

d) 7-(2-{1-[1-(3-Chloro-phenyl)-2-ethoxycarbonyl-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from (3-chloro-phenyl)-propynoic acid ethyl ester and 7-[2-(1H-Indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (d1), in 90% yield as an E/Z isomeric mixture. ¹H NMR (CDCl₃) [E/Z mixture] δ 7.36-7.24 (m, 2H), 7.14-7.6.70 (m, 8H), 6.56 (s, 0.5H), 6.48 (m, 0.5H), 6.21 (s, 0.5H), 6.14 (s, 0.5H), 4.37 (m, 2H), 4.12 (q, 2H, J=6.8 Hz), 3.76 (m, 2H), 3.20 (t, 2H, J=6.8 Hz), 2.73 (t, 2H, J=6.8 Hz), 1.92 (m, 2H), 1.52 (s, 9H), 1.26 (t, 3H, J=7.2 Hz). Mass Spectrum (LCMS, ESI) calculated for C₂₉H₂₉ClN₃O₃502.2 (M-Boc+1); found 502.4.

e) 7-(2-{1-[1-(3-Chloro-phenyl)-2-ethoxycarbonyl-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-(2-{1-[1-(3-chloro-phenyl)-2-ethoxycarbonyl-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 18, step (e), in 86% yield. ¹H NMR (CDCl₃) δ 7.23-7.01 (m, 7H), 6.94 (m, 1H), 6.85 (d, 1H, J=7.6 Hz), 6.75 (dd, 1H, J=2.4, 6.5 Hz), 6.37 (d, 1H, J=4.0 Hz), 5.89 (t, 1H, J=7.5 Hz), 4.28 (q, 2H, J=6.9 Hz), 3.98 (q, 2H, J=7.1 Hz), 3.67 (m, 2H), 3.14 (m, 4H), 2.64 (t, 2H, J=6.6 Hz), 1.83 (m, 2H), 1.43 (s, 9H), 1.02 (t, 3H, J=7.1 Hz). Mass Spectrum (LCMS, ESI) calculated for C₂₉H₃₁ClN₃O₃504.2 (M-Boc+H); found 504.4.

f) 3-(3-Chloro-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester

The title compound was synthesized from 7-(2-{1-[1-(3-Chloro-phenyl)-2-ethoxycarbonyl-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (f), in 63% yield. ¹H NMR (CDCl₃) δ 7.26-7.00 (m, 8H), 6.82 (dd, 1H, J=2.4, 6.4 Hz), 6.46 (m, 1H), 5.97 (t, 1H, J=7.6 Hz), 4.85 (bs, 1H), 4.30 (t, 2H, J=7.2 Hz), 4.06 (q, 2H, J=7.2 Hz), 3.39 (m, 2H), 3.31-3.18 (m, 2H), 3.03 (t, 1H, J=6.8 Hz), 2.68 (t, 2H, J=6.4 Hz), 1.89 (m, 2H), 1.10 (t, 3H, J=7.2 Hz).

g) 3-(3-Chloro-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

The title compound was synthesized from 3-(3-chloro-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester using the procedure described in Example 18, step (g), in 30% yield. ¹H NMR (CDCl₃/CD₃OD) δ 7.24 (d, 1H, J=3.2 Hz), 7.13 (d, 1H, J=7.3 Hz), 7.03 (m, 4H), 6.85 (m, 2H), 6.60 (m, 1H), 6.33 (m, 2H), 5.85 (m, 1H), 3.30-3.21 (m, 4H), 3.00 (m, 2H), 2.81 (m, 2H), 2.57 (m, 2H), 1.76 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₇H₂₇ClN₃O 476.2 (M+H); found 476.9.

EXAMPLE 32

3-Naphthalen-2-yl-3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

a) 3-Naphthalen-2-yl-3-oxo-propionic acid ethyl ester

Diethylcarbonate (3.90 mL, 33.0 mmol) was added to a slurry of sodium hydride (1.30 g, 33.0 mmol) in toluene (100 mL) at room temperature under Ar. A solution of 2-acetophenone (5.00 g, 29.0 mmol) in toluene (30 mL) was added immediately and the mixture was heated at reflux for 2 hours. After cooling to room temperature, the mixture was poured over ice/water and extracted with ethyl acetate. The organic extracts were dried over magnesium sulfate and the solvent was removed under reduced pressure. The crude product was purified over silica (2.5% ethyl acetate/hexanes) to give the title compound (4.45 g, 55%, 3:1 mixture of keto/enol form) as clear oil. ¹H NMR (CDCl₃) δ 12.69 (s, 0.25H, enol), 8.46 (d, 0.75H, J=0.8 Hz), 8.37 (d, 0.25H, J=0.8 Hz), 7.98 (m, 2H), 7.88(m, 3H), 7.78 (m, 0.25H), 7.57 (m, 2.75H), 5.82 (s, 0.25H, enol), 4.27 (m, 2H), 4.13 (s, 1.5H, keto) 1.36 (t, 0.75H, J=8.0 Hz), 1.27 (t, 1.25H, J=8.0 Hz).

b) Naphthalene-2-yl-propynoic acid ethyl ester

Triflic anhydride (2.9 mL, 17 mmol) was added dropwise to a solution of triphenylphosphine oxide (4.8 g, 17 mmol) in 1,2-dichloroethane (40 mL) at 0° C. The resulting suspension was stirred for 15 minutes, followed by the dropwise addition of a solution of 3-naphthalen-2-yl-3-oxo-propionic acid ethyl ester (3.2 g, 12 mmol) in 1,2-dichloroethane (40 mL). After the addition was complete, triethylamine (4.0 mL, 29 mmol) was added and the reaction mixture was heated at reflux for 1 hr. The solution was cooled to room temperature, washed with water, and dried (MgSO₄). The solvent was removed under reduced pressure, and the product was purified via column chromatography with silica eluting with hexane/ethyl acetate (9/1) to yield naphthalene-2-yl-propynoic acid ethyl ester (1.15 g, 37% yield) as a yellow oil. ¹H NMR (CDCl₃) δ 8.25 (s, 1H), 7.95 (m, 3H), 7.66 (m, 3H), 4.46 (m, 2H), 1.51 (t, 3H, J=8.0 Hz).

c) 7-{2-[1-(2-Ethoxycarbonyl-1-naphthalen-2-yl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from naphthalene-2-yl-propynoic acid ethyl ester and 7-[2-(1H-indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (d1), in 88% yield as an E/Z isomeric mixture. ¹H NMR (CDCl₃) δ 7.85 (m, 4H), 7.50 (m, 2.6H), 7.33 (m, 1.6H), 7.12 (m, 1.8H), 6.95 (m, 1.4H), 6.74 (m, 1.6H), 6.61 (d, 0.6H, J=0.4 Hz), 6.50 (d, 0.4H, J=0.4 Hz), 6.35 (s, 0.6H), 6.23 (s, 0.4 HZ), 4.36 (t, 2H, J=8.0 Hz), 4.05 (m, 2H), 3.76 (m, 2H), 3.20 (t, 2H, J=8.0), 2.73 (t, 2H, J=8.0 Hz), 1.92 (m, 2H), 1.51 (s, 9H), 1.07 (m, 3H).

d) 7-{2-[1-(2-Ethoxycarbonyl-1-naphthalen-2-yl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-{2-[1-(2-ethoxycarbonyl-1-naphthalen-2-yl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 18, step (e), in 80% yield. ¹H NMR (CDCl₃) δ 7.76 (m, 3H), 7.67 (s, 1H), 7.45 (m, 2H), 7.29 (d, 1H, J=7.6 Hz), 7.23 (m, 3H), 7.09 (d, 1H, J=2.4 Hz), 6.92 (d, 2H, J=8.0 Hz), 6.80 (dd, 1H, J=2.4, 6.4 Hz), 6.18 (t, 1H, J=7.6 Hz), 4.35 (t, 2H, J=7.2 Hz), 4.08 (m, 2H), 3.75 (m, 2H), 3.36 (m, 2H), 3.19 (t, 2H, J=6.8 Hz), 2.71 (t, 2H, J=6.8 Hz), 1.91 (m, 2H), 1.50 (s, 9H), 1.10 (t, 3H, J=6.8 Hz).

e) 3-Naphthalen-2-yl-3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester

The title compound was synthesized from 7-{2-[1-(2-ethoxycarbonyl-1-naphthalen-2-yl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (f), in 71% yield. ¹H NMR (CDCl₃) δ 7.76 (m, 3H), 7.67 (s, 1H), 7.46 (m, 2H), 7.23 (m, 3H), 7.08(m, 2H), 6.80 (dd, 1H, J=2.4, 6.4 Hz), 6.45 (m, 2H), 6.17 (t, 1H, J=Hz), 5.07 (s, 1H), 4.27 (t, 2H, J=6.8 Hz), 4.05 (m, 2H), 3.38 (m, 4H), 3.05 (t, 2H, J=6.8 Hz), 2.67 (t, 2H, J=6.4 Hz), 1.88 (m, 2H), 1.09 (t, 3H, J=7.2 Hz).

f) 3-Naphthalen-2-yl-3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

The title compound was synthesized from 3-naphthalen-2-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester using the procedure described in Example 18, step (g), in 34% yield. ¹H NMR (DMSO-d₆) δ 7.90 (s, 1H), 7.84 (m, 3H), 7.71 (d, 1H, J=3.2 Hz), 7.47 (m, 4H), 7.02 (m, 2H), 6.68 (dd, 1H, J=2.4, 6.4 Hz), 6.39 (d, 1H, J=2.8 Hz), 6.34 (d, 1H, J=7.2 Hz), 6.31 (s, 1H), 6.10 (m, 1H), 4.18 (t, 2H, J=6.8 Hz), 3.59 (m, 2H), 3.23 (m, 2H), 2.85 (t, 2H, J=6.8 Hz), 2.50 (t, 2H, J=2.0 Hz), 1.75 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₃₁H₃₀N₃O₃: 492.2 (M+H), found: 492.3.

EXAMPLE 33

3-(2-Chloro-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

a) (2-Chloro-phenyl)-propynoic acid methyl ester

The title compound was synthesized from commercially available 3-(2-chloro-phenyl)-3-oxo-propionic acid methyl ester using the procedure described in Example 32, step (b), in 71% yield. ¹H NMR (CDCl₃) δ 7.53 (dd, 1H, J=1.6, 6.0 Hz), 7.36 (m, 1H), 7.30 (dt, 1H, J=1.6, 5.7 Hz), 7.19 (dt, 1H, J=1.3, 6.4 Hz), 3.78 (s, 3H).

b) 7-(2-{1-[1-(2-Chloro-phenyl)-2-methoxycarbonyl-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from (2-chloro-phenyl)-propynoic acid methyl ester and 7-[2-(1H-Indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (d1), in 43% yield as an E/Z isomeric mixture. ¹H NMR (CDCl₃) [E/Z mixture] δ 7.54-7.30 (m, 6H), 7.18 (m, 1H), 6.95 (m, 1H), 6.83 (m, 1H), 6.68-6.50 (m, 2H), 6.35 (s, 0.33H), 5.95 (s, 0.67H), 4.39 (m, 2H), 3.78 (m, 2H), 3.64 (s, 3H), 3.22 (m, 2H), 2.73 (m, 2H), 1.92 (m, 2H), 1.52 (s, 9H). Mass Spectrum (LCMS, ESI) calculated for C₂₈H₂₇ClN₃O₃488.2 (M-Boc+H); found 488.4.

c) 7-(2-{1-[1-(2-Chloro-phenyl)-2-methoxycarbonyl-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-(2-{1-[1-(2-chloro-phenyl)-2-methoxycarbonyl-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 18, step (e), in 85% yield. ¹H NMR (CDCl₃) δ 7.41-7.25 (m, 3H), 7.22-6.79 (m, 7H), 6.50 (m, 1H), 6.40 (m, 1H), 4.37 (t, 2H, J=7.2 Hz), 3.76 (m, 2H), 3.61 (s, 3H), 3.20 (m, 4H), 2.75 (m, 2H), 1.94 (m, 2H), 1.50 (s, 9H).

d) 3-(2-Chloro-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid methyl ester

The title compound was synthesized from 7-(2-{1-[1-(2-chloro-phenyl)-2-methoxycarbonyl-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (f), in 45% yield. ¹H NMR (CDCl₃) δ 7.31 (m, 1H), 7.20-7.00 (m, 6H), 6.83 (dd, 1H, J=1.7, 6.0 Hz), 6.74 (dd, 1H, J=1.7, 6.0 Hz), 6.39 (m, 2H), 6.32 (m, 1H), 4.82 (s, 1H), 4.21 (m, 2H), 3.54 (s, 3H), 3.32 (m, 2H), 3.17 (m, 2H), 2.95 (m, 2H), 2.62 (m, 2H), 1.82 (m, 2H).

e) 3-(2-Chloro-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

The title compound was synthesized from 3-(2-chloro-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid methyl ester using the procedure described in Example 18, step (g), in 33% yield. ¹H NMR (CDCl₃) δ 10.4 (bs, 1H), 7.60 (d, 1H, J=2.8 Hz), 7.37 (dd, 1H, J=1.2, 6.8 Hz), 7.18-7.04 (m, 4H), 6.85 (dd, 1H, J=1.4, 6.3 Hz), 6.78 (d, 1H, J=2.3 Hz), 6.59 (dd, 1H, J=2.2, 6.7 Hz), 6.49 (m, 2H), 6.23 (d, 1H, J=7.2 Hz), 3.42 (m, 3H), 3.39-3.08 (m, 4H), 2.62 (t, 2H, J=6.2 Hz), 2.51 (m, 1H), 3.35 (m, 1H), 1.82 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₇H₂₇ClN₃O₃476.2 (M+H); found: 476.31.

EXAMPLE 34

3-Naphthalen-1-yl-3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

a) 3-Naphthalen-1-yl-3-oxo-propionic acid ethyl ester

The title compound was synthesized from commercially available 1-acetonapthrone using the procedure described in Example 32, step (a), in 25% yield as a 3:1 mixture of keto/enol tautomers. ¹H NMR (CDCl₃) δ 12.73 (s, 0.25H, enol), 8.75 (dd, 0.75H, J=4.0, 8.0 Hz, keto), 8.36 (dd, 0.25H, J=4.0, 8.0 Hz, enol), 8.03 (d, 0.75H, J=8.0 Hz), 7.90 (m, 2.75H), 7.64 (m, 1.5H), 7.54 (m, 3H), 5.50 (s, 0.25H, enol), 4.32 (m, 0.5H), 4.20 (m, 1.5H) 4.11 (s, 1.5H, keto), 1.36 (t, 0.75H, J=8.0 Hz), 1.21 (t, 1.25, J=8.0 Hz).

b) Naphthalene-1-yl-propynoic acid ethyl ester

The title compound was synthesized from 3-naphthalen-1-yl-3-oxo-propionic acid ethyl ester using the procedure described in Example 32, step (b), in 25% yield. ¹H NMR (CDCl₃) δ 8.35 (dd, 1H, J=0.4, 1.4 Hz), 7.95 (d, 1H, J=8.0 Hz), 7.86 (m, 2H), 7.61 (m, 2H), 7.46(m, 1H), 4.36 (m, 2H), 1.41 (t, 3H, J=8.0 Hz).

c) 7-{2-[1-(2-Ethoxycarbonyl-1-naphthalen-1-yl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from naphthalene-1-yl-propynoic acid ethyl ester and 7-[2-(1H-indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (d1), in 37% yield as an E/Z isomeric mixture. ¹H NMR (CDCl₃) δ 8.32 (m, 1H), 7.85 (m, 2H), 7.47 (m, 3H), 7.27 (m, 2.5H), 6.95 (m, 3H), 6.72 (m, 1.5H), 6.58 (m, 0.5H), 6.35 (m, 1.5H), 4.27 (m, 2H), 4.03 (m, 2H), 3.69 (m, 2H), 3.14 (m, 2H), 2.67 (m, 2H), 1.86 (m, 2H), 1.45 (s, 9H), 1.18 (m, 3H).

d) 7-{2-[1-(2-Ethoxycarbonyl-1-naphthalen-1-yl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-{2-[1-(2-ethoxycarbonyl-1-naphthalen-1-yl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 18, step (e), in 23% yield. ¹H NMR (CDCl₃) δ 8.04 (m, 1H), 7.88 (m, 1H), 7.81 (d, 1H, J=8.4 Hz), 7.50 (m, 2H), 7.40 (t, 1H, J=7.6 Hz), 7.28 (m, 4H), 7.10 (d, 1H, J=2.4 Hz), 6.96 (t, 1H, J=7.6 Hz), 6.83 (m, 2H), 6.40 (d, 1H, J=8.0 Hz), 4.35 (t, 2H, J=8.0 Hz), 4.06 (m, 2H), 3.77 (m, 2H), 3.36 (m, 2H), 3.21 (t, 2H, J=8.0 Hz), 2.74(t, 2H, J=8.0 Hz), 1.92 (m, 2H), 1.50, (s, 9H), 1.11 (t, 3H, J=8.0 Hz).

e) 3-Naphthalen-1-yl-3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester

The title compound was synthesized from 7-{2-[1-(2-ethoxycarbonyl-1-naphthalen-1-yl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (f), in 90% yield. ¹H NMR (CDCl₃) δ 8.02 (m, 1H), 7.89 (m, 1H), 7.81 (d, 1H, J=8.0 Hz), 7.50 (m, 2H), 7.39 (t, 1H, J=7.6 Hz), 7.28(m, 2H), 7.10 (m, 3H), 6.85 (m, 2H), 6.48 (m, 1H), 6.41 (d, 1H, J=3.2 Hz), 4.92 (s, 1H), 4.30 (t, 2H, J=7.2 Hz), 4.07 (m, 2H), 3.37 (m, 4H), 3.05 (t, 2H, J=6.4 Hz), 2.68 (t, 2H, J=6.4 Hz), 1.88 (m, 2H), 1.09 (t, 3H, J=7.2 Hz).

f) 3-Naphthalen-1-yl-3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

The title compound was synthesized from 3-naphthalen-1-yl-3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester using the procedure described in Example 18, step (g), in 52% yield. ¹H NMR (DMSO-d₆) δ 8.17 (d, 1H, J=8.4 Hz), 7.96 (m, 1H), 7.88 (d, 1H, J=8.0 Hz), 7.55 (m, 3H), 7.45(t, 1H, J=7.2 Hz), 7.30 (m, 2H), 7.03 (m, 2H), 6.68 (m, 2H), 6.36 (m, 2H), 6.31 (s, 1H), 4.19 (t, 2H, J=7.2 Hz), 3.60 (m, 2H) 3.24 (m, 2H), 2.86 (t, 2H, J=7.2 Hz), 2.60 (t, 2H, J=6.4 Hz), 1.74 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₃₁H₃₀N₃O₃: 492.2 (M+H), found: 492.3.

EXAMPLE 35

3-(4-Fluoro-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

a) (4-Fluoro-phenyl)-propynoic acid methyl ester

The title compound was synthesized from 3-(4-fluoro-phenyl)-3-oxo-propionic acid methyl ester using the procedure described in Example 32, step (b), in 91% yield. ¹H NMR (CDCl₃) δ 7.59 (m, 2H), 7.08 (m, 2H), 3.84 (s, 3H).

b) 7-(2-{1-[1-(4-Fluoro-phenyl)-2-methoxycarbonyl-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from (4-fluoro-phenyl)-propynoic acid methyl ester and 7-[2-(1H-Indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 17, step (a), in 73% yield as an E/Z isomeric mixture. ¹H NMR (CDCl₃) [E/Z mixture] δ 7.43 (m, 1H), 7.30 (m, 2H), 7.00-7.20 (m, 3), 6.94 (m, 1H), 6.50-6.90 (m, 3H), 6.12 (s, 1H), 4.36 (m, 2H), 3.75 (m, 2H), 3.7 and 3.6 (s, 3H), 3.20 (m, 2H), 2.75 (m, 2H), 1.90 (m, 2H), 1.50 (s, 9H). Mass Spectrum (LCMS, ESI) calculated for C₃₃H₃₅FN₃O₅572.3 (M+H); found 472.3 (M-Boc+H).

c) 7-(2-{1-[1-(4-Fluoro-phenyl)-2-methoxycarbonyl-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-(2-{1-[1-(4-fluoro-phenyl)-2-methoxycarbonyl-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 18, step (e), in 57% yield. ¹H NMR (CDCl₃) δ 7.34 (d, 1H), 7.15 (m, 4H), 6.95 (m, 4H), 6.80 (d, 1H), 6.45 (d, 1H), 6.00 (t, 1H), 4.40 (t, 2H), 7.50 (t, 2H), 3.60 (s, 3H), 3.15-3.30 (m, 4H), 2.72 (t, 2H), 1.90 (m, 2H), 1.50 (s, 9H). Mass Spectrum (LCMS, ESI) calculated for C₃₃H₃₇FN₃O₅574.3 (M+H); found 474.2 (M-Boc+H).

d) 3-(4-Fluoro-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid methyl ester

The title compound was synthesized from 7-(2-{1-[1-(4-fluoro-phenyl)-2-methoxycarbonyl-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (f), in 83% yield. ¹H NMR (CDCl₃) δ 7.04 (m, 4H), 6.86 (m, 2H), 6.87 (t, 2H), 6.75 (dd, J=2.4 and 8.9 Hz, 1H), 6.36 (m, 2H), 5.90 (t, 1H), 4.91 (br, 1H), 4.20 (t, J=7.0 Hz, 2H), 3.53 (s, 3H), 3.30 (m, 2H), 3.20 (m, 2H), 2.95 (t, J=7.0 Hz, 2H), 2.60 (t, 2H), 1.82 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₈H₂₉FN₃O₃474.2 (M+H); found 474.3.

e) 3-(4-Fluoro-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

The title compound was synthesized from 3-(4-fluoro-phenyl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid methyl ester using the procedure described in Example 16, step (g), in 64% yield. ¹H NMR (CDCl₃) δ 10.47 (br, 1H), 10.39 (d, J=3.2 Hz, 1H), 7.15 (d, J=8.9 Hz, 1H), 7.04 (m, 3H), 6.83 (m, 3H), 6.54 (q, 1H), 6.39 (d, J=3.0 Hz, 1H), 6.20 (d, J=7.2 Hz), 6.02 (q, 1H), 3.57 (br, 1H), 3.40 (br, 1H), 3.31 (t, J=5.3 Hz, 2H), 3.05-3.18 (m, 2H), 2.43-2.58 (m, 4H), 1.76 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₇H₂₇FN₃O₃460.2 (M+H); found 460.2.

EXAMPLE 36

3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-(3-trifluoromethyl-phenyl)-propionic acid

a) (3-Trifluoromethyl-phenyl)-propynoic acid methyl ester

The title compound was synthesized from commercially available 3-oxo-3-(3-trifluoromethyl-phenyl)-propionic acid methyl ester using the procedure described in Example 32, step (b), in 100% yield. ¹H NMR (CDCl₃) δ 7.84 (s, 1H), 7.75 (q, 1H), 7.50 (t, 1H), 3.90 (s, 3H).

b) 7-(2-{1-[2-Methoxycarbonyl-1-(3-trifluoromethyl-phenyl)-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from (3-trifluoromethyl-phenyl)-propynoic acid methyl ester using the procedure described in Example 16, step (d2), in 47% yield. ¹H NMR (CDCl₃) δ 7.77 (m, 1H), 7.67 (s, 1H), 7.60 (m, 2H), 7.34 (d, J=7.7 Hz, 1H), 7.13 (m, 2H), 6.96 (d, J=7.6 Hz, 1H), 6.85 (m, 2H), 6.54 (dd, J=3.5 and 0.6 Hz, 1H), 6.26 (s, 1H), 4.41 (m, 2H), 3.78 (m, 2H), 3.67 (s, 3H), 3.23 (t, J=6.8 Hz, 2H), 2.76 (t, J=6.7 Hz, 2H), 1.94 (m, 2H), 1.54 (s, 9H). Mass Spectrum (LCMS, ESI) calculated for C₃₄H₃₅F₃N₃O₅622.3 (M+H); found: 522.4 (M-Boc+H).

c) 7-(2-{1-[2-Methoxycarbonyl-1-(3-trifluoromethyl-phenyl)-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-(2-{1-[2-methoxycarbonyl-1-(3-trifluoromethyl-phenyl)-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 18, step (e), in 70% yield. ¹H NMR (CDCl₃) δ 7.45 (m, 3H), 7.23 (m, 1H), 7.17 (m, 1H), 7.10 (m, 1H), 7.06 (m, 1H), 6.87 (m, 1H), 6.75 (m, 1H), 6.40 (m, 1H), 5.97 (t, J=7.5 Hz, 1H), 4.28 (m, 2H), 3.72 (m, 2H), 3.54 (s, 3H), 3.07-3.29 (m, 4H), 2.66 (m, 2H), 1.84 (m, 2H), 1.45 (s, 9H). Mass Spectrum (LCMS, ESI) calculated for C₃₄H₃₇F₃N₃O₅624.3 (M+H); found: 524.4 (M-Boc+H).

d) 3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-(3-trifluoromethyl-phenyl)-propionic acid methyl ester

The title compound was synthesized from 7-(2-{1-[2-methoxycarbonyl-1-(3-trifluoromethyl-phenyl)-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (f), in 37% yield. ¹H NMR (CDCl₃) δ 7.43 (m, 2H), 7.32 (t, J=7.8 Hz, 1H), 7.19 (m, 1H), 7.01-7.13 (m, 3H), 6.75 (dd, J=2.3 and 8.9 Hz, 1H), 6.39 (m, 2H), 5.98 (m, 1H), 4.22 (m, 2H), 3.54 (s, 3H), 3.16-3.40 (m, 4H), 2.96 (m, 2H), 2.62 (t, J=6.2 Hz, 2H), 1.82 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₉H₂₉F₃N₃O₃524.2 (M+H); found 524.4.

e) 3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-(3-trifluoromethyl-phenyl)-propionic acid

The title compound was synthesized from 3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-(3-trifluoromethyl-phenyl)-propionic acid methyl ester using the procedure described in Example 16, step (g), in 55% yield. ¹H NMR (CDCl₃) δ 10.5 (br, 1H), 7.48 (m, 3H), 7.35 (t, J=7.6 Hz, 1H), 7.25 (m, 3H), 7.15 (dd, J=7.0 and 8.5 Hz, 1H), 6.88 (d, J=2.2 Hz, 1H), 6.63 (dd, J=2.2 and 8.9 Hz, 1H), 6.50 (s, 1H), 6.30 (d, J=7.3 Hz, 1H), 6.18 (q, 1H), 3.73 (m, 1H), 3.55 (m, 1H), 3.40 (m, 2H), 3.13-3.31 (m, 2H), 2.76 (m, 4H), 1.85 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₈H₂₇F₃N₃O₃510.2 (M+H); found 510.3 (M⁺+1, 100%).

EXAMPLE 37

3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-(4-trifluoromethyl-phenyl)-propionic acid

a) (4-Trifluoromethyl-phenyl)-propynoic acid methyl ester

The title compound was synthesized from commercial available 3-oxo-3-(4-trifluoromethyl-phenyl)-propionic acid methyl ester using the procedure described in Example 32, step (b), in 84% yield. ¹H NMR (CDCl₃) δ 7.70 (m, 4H), 3.85 (s, 3H).

b) 7-(2-{1-[2-Methoxycarbonyl-1-(4-trifluoromethyl-phenyl)-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from (4-trifluoromethyl-phenyl)-propynoic acid methyl ester using the procedure described in Example 16, step (d2), in 62% yield as an E/Z isomeric mixture. ¹H NMR (CDCl₃) [E/Z mixture] δ 7.62 (d, J=8.1 Hz, 1H), 7.54 (d, J=8.2 Hz, 1H), 7.44 (d, J=7.8 Hz, 1H), 7.33 (m, 1H), 7.23 (m, 1H), 7.05 (m, 2H), 6.88 (m, 1H), 6.75 (m, 1H), 6.53 (m, 1H), 6.17 (s, 1H), 4.31 (m, 2H), 3.69 (m, 2H), 3.53 (s, 3H), 3.13 (m, 2H), 2.66 (t, J=6.6 Hz, 2H), 1.85 (m, 2H), 1.44 (s, 9H). Mass Spectrum (LCMS, ESI) calculated for C₃₄H₃₅F₃N₃O₅622.3 (M+H); found: 522.4 (M-Boc+H).

c) 7-(2-{1-[2-Methoxycarbonyl-1-(4-trifluoromethyl-phenyl)-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-(2-{1-[2-methoxycarbonyl-1-(4-trifluoromethyl-phenyl)-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 18, step (e), in 36% yield, and was used directly in the next reaction without purification.

d) 3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-(4-trifluoromethyl-phenyl)-propionic acid methyl ester

The title compound was synthesized from 7-(2-{1-[2-methoxycarbonyl-1-(4-trifluoromethyl-phenyl)-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (f), in 33% yield. ¹H NMR (CDCl₃) δ 7.60 (d, 2H), 7.30 (d, 2H), 7.15 (d, 1H), 7.10 (m, 3H), 6.83 (m, 1H), 6.50 (m, 2H), 6.08 (t, 1H), 5.15 (br, 1H), 4.33 (t, 2H), 3.60 (s, 3H), 3.25-3.45 (m, 4H), 3.10 (m, 2H), 2.75 (m, 2H), 1.90 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₉H₂₉F₃N₃O₃524.2 (M+H); found: 524.4.

e) 3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-(4-trifluoromethyl-phenyl)-propionic acid

The title compound was synthesized from 3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-(4-trifluoromethyl-phenyl)-propionic acid methyl ester using the procedure described in Example 16, step (g), in 67% yield. ¹H NMR (CDCl₃) δ 10.60 (br, 1H), 7.40 (d, J=8.0 Hz, 3H), 7.15 (d, J=8.1 Hz, 2H), 7.08 (m, 2H), 6.81 (d, J=1.9 Hz, 1H), 6.56 (dd, J=2.3 and 8.9 Hz, 1H), 6.40 (s, 1H), 6.37 (m, 1H), 6.08 (m, 1H), 3.65 (br, 1H), 6.45 (br, 3H), 6.1-6.3 (m, 2H), 2.60 (m, 4H), 1.85 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₈H₂₇F₃N₃O₃510.2 (M+H); found 510.3.

EXAMPLE 38

3-Pyridin-3-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

a) Pyridin-3-yl-propynoic acid methyl ester

The title compound was synthesized from 3-oxo-3-pyridin-3-yl-propionic acid methyl ester using the procedure described in Example 32, step (b), in 80% yield. ¹H NMR (CDCl₃) δ 8.80 (dd, J=0.7 and 2.0 Hz, 1H), 8.66 (dd, J=1.7 and 4.9 Hz, 1H), 7.88 (m, 1H), 7.36 (m, 1H), 3.86 (s, 3H).

b) 7-{2-[1-(2-Methoxycarbonyl-1-pyridin-3-yl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from pyridin-3-yl-propynoic acid methyl ester using the procedure described in Example 17, step (a), in 78% yield as an E/Z isomeric mixture. Mass Spectrum (LCMS, ESI) calculated for C₃₂H₃₅N₄O₅555.3 (M+H); found 455.4 (M-Boc+H).

c) 7-{2-[1-(2-Methoxycarbonyl-1-pyridin-3-yl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized 7-{2-[1-(2-methoxycarbonyl-1-pyridin-3-yl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 17, step (b), in 45% yield. ¹H NMR (CDCl₃) δ 8.47 (s, 1H), 8.42 (m, 1H), 7.28 (d, J=8.0 Hz, 1H), 7.22 (J=7.6 Hz, 1H), 7.02-7.13 (m, 4H), 6.86 (d, J=7.6 Hz, 1H), 6.74 (d, J=8.8 Hz, 1H), 6.39 (d, J=2.1 Hz, 1H), 5.96 (t, J=7.6 Hz, 1H), 4.28 (m, 2H), 3.67 (t, 2H), 3.54 (s, 3H), 3.24 (m, 2H), 3.12 (m, 2H), 2.65 (t, J=6.5 Hz, 2H), 1.84 (m, 2H), 1.43 (s, 9H). Mass Spectrum (LCMS, ESI) calculated for C₃₂H₃₇N₄O₅557.3 (M+H); found 457.4 (M-Boc+H), 557.1.

d) 3-Pyridin-3-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid methyl ester

The title compound was synthesized from 7-{2-[1-(2-methoxycarbonyl-1-pyridin-3-yl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (f), in 26% yield. ¹H NMR (CDCl₃) δ 8.43-8.47 (br, 2H), 7.29 (d, J=8.0 Hz, 1H), 7.00-7.14 (m, 5H), 6.73 (dd, J=2.4 and 8.9 Hz, 1H), 6.41 (d, J=7.1 Hz, 2H), 5.96 (t, J=7.6 Hz, 1H), 5.37 (br, 1H), 4.19 (t, J=6.7 Hz, 2H), 3.54 (s, 3H), 3.33 (m, 2H), 3.24 (m, 2H), 2.97 (t, J=6.7 Hz, 2H), 2.62 (t, J=6.3 Hz, 2H), 1.82 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₇H₂₉N₄O₃457.2 (M+H); found 457.4.

e) 3-Pyridin-3-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

The title compound was synthesized from 3-pyridin-3-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid methyl ester using the procedure described in Example 14, step (e), in 36% yield. ¹H NMR (CDCl₃) δ 10.50 (br, 1H), 8.57 (d, 1H), 8.42 (d, 1H), 7.50 (1H), 7.35 (d, 1H), 7.20 (m, 2H), 6.95 (d, 1H), 6.88 (s, 1H), 6.60 (d, 1H), 6.50 (s, 1H), 6.28 (d, 1H), 6.22 (m, 1H), 5.10 (br, 1H), 3.70 (m, 1H), 3.50 (m, 1H), 3.45 (m, 2H), 3.10-3.30 (m, 2H), 2.60 (m, 2H), 2.50 (m, 2H), 1.85 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₆H₂₇N₄O₃443.2 (M+H); found 443.3.

EXAMPLE 39

3-Pyridin-2-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

a) Pyridin-2-yl-propynoic acid ethyl ester

The title compound was synthesized from commercially available 3-oxo-3-pyridin-2-yl-propionic acid methyl ester using the procedure described in Example 32, step (b), in 76% yield. ¹H NMR (CDCl₃) δ 8.66 (d, J=4.8 Hz, 1H), 7.73 (m, 1H), 7.59 (d, J=7.8 Hz, 1H), 7.36 (m, 1H), 4.31 (q, J=7.1 Hz, 2H), 1.35 (t, J=7.1 Hz, 3H).

b) 7-{2-[1-(2-Ethoxycarbonyl-1-pyridin-2-yl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from pyridin-2-yl-propynoic acid ethyl ester using the procedure described in Example 17, step (a), in 90% yield as an E/Z isomeric mixture. Mass Spectrum (LCMS, ESI) calculated for C₃₃H₃₇N₄O₅569.3 (M+H); found 469.3 (M-Boc+H).

c) 7-{2-[1-(2-Methoxycarbonyl-1-pyridin-2-yl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-{2-[1-(2-ethoxycarbonyl-1-pyridin-2-yl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 18, step (e), in 90% yield. Transesterification occurred during the reduction, resulting in a mixture of ethyl and methyl esters. ¹H NMR (CDCl₃) δ 8.49 (br, 2H), 7.30 (m, 2H), 7.15 (d, J=3.2 Hz, 1H), 7.06 (m, 1H), 6.98 (m, 2H), 6.93 (m, 1H), 6.80 (d, J=2.4 Hz, 1H), 6.47 (d, J=3.3 Hz, 1H), 5.99 (t, 7.5 Hz, 1H), 4.34 (t, J=6.9 Hz, 2H), 3.73 (t, J=6.0 Hz, 2H), 3.61 (s, 3H), 3.25 (m, 2H), 3.18 (m, 2H), 2.70 (m, 2H), 1.91 (m, 2H), 1.49 (s, 9H). Mass Spectrum (LCMS, ESI) calculated for C₃₂H₃₆N₄O₅557.3 (M+H); found 457.4 (M-Boc+H), 557.0.

d) 3-Pyridin-2-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid methyl ester

The title compound was synthesized from 7-{2-[1-(2-methoxycarbonyl-1-pyridin-2-yl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (f), in 58% yield. Mass Spectrum (LCMS, ESI) calculated for C₂₇H₂₉N₄O₃457.2 (M+H); found 457.4.

e) 3-Pyridin-2-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

The title compound was synthesized from 3-pyridin-2-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid methyl ester using the procedure described in Example 16, step (g), in 14% yield. ¹H NMR (CDCl₃) δ 10.29 (br, 1H), 8.41 (br, 1H), 7.35 (d, J=2.8 Hz, 1H), 6.92-7.17 (m, 3H), 6.81 (d, J=2.0 Hz, 1H), 6.55 (dd, J=2.0 and 8.8 Hz, 1H), 6.43 (d, J=2.8 Hz, 1H), 6.26 (m, 1H), 6.02 (br, 1H), 3.66 (br, 1H), 3.58 (m, 1H), 3.34 (m, 2H), 3.13 (m, 2H), 2.59 (m, 4H), 1.81 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₆H₂₇N₄O₃443.2 (M+H); found 443.3.

EXAMPLE 40

3-Pyridin-4-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-acrylic acid

a) Pyridin-4-yl-propynoic acid ethyl ester

The title compound was synthesized from commercially available 3-oxo-3-pyridin-4-yl-propionic acid ethyl ester using the procedure described in Example 32, step (b), in 65% yield. ¹H NMR (CDCl₃) δ 8.67 (dd, J=1.5 and 4.5 Hz, 2H), 7.42 (dd, J=1.5 and 4.5, 2H), 4.33 (q, J=7.2 Hz, 2H), 1.37 (t, J=7.2 Hz, 3H).

b) 7-{2-[1-(2-Ethoxycarbonyl-1-pyridin-4-yl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from pyridin-4-yl-propynoic acid ethyl ester using the procedure described in Example 17, step (a), in 90% yield as an E/Z isomeric mixture. Mass Spectrum (LCMS, ESI) calculated for C₃₃H₃₅N₄O₅569.3 (M+H); found 469.4 (M-Boc+H).

c) 7-{2-[1-(2-Ethoxycarbonyl-1-pyridin-4-yl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-{2-[1-(2-ethoxycarbonyl-1-pyridin-4-yl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 14, step (b), in 26% yield. Mass Spectrum (LCMS, ESI) calculated for C₃₃H₃₉N₄O₅571.3 (M+H); found 471.4 (M-Boc+H).

d) 3-Pyridin-4-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-acrylic acid ethyl ester

The title compound was synthesized from 7-{2-[1-(2-ethoxycarbonyl-1-pyridin-4-yl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (f), in 77% yield as an E/Z isomeric mixture. Mass Spectrum (LCMS, ESI) calculated for C₂₈H₂₉N₄O₃469.2 (M+H); found 469.4.

e) 3-Pyridin-4-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-acrylic acid

The title compound was synthesized from 3-pyridin-4-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-acrylic acid ethyl ester using the procedure described in Example 14, step (e), in 23% yield as a single isomer. ¹H NMR (CDCl₃) δ 10.48 (br, 1H), 8.48 (d, J=5.6 Hz, 2H), 7.15 (d, J=3.2, 1H), 7.08 (d, J=7.3 Hz, 1H), 7.03 (d, J=5.9, 2H), 6.88 (d, 8.9 Hz, 1H), 6.73 (m, 2H), 6.59 (d, 2.2 Hz, 1H), 6.57 (d, J=2.2 Hz, 1H), 6.51 (d, J=3.0 Hz, 1H), 6.25 (d, J=7.3 Hz, 1H), 3.48 (br, 2H), 3.35 (br, 2H), 2.59 (m, 2H), 2.44 (br, 2H), 1.80 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₆H₂₅N₄O₃441.19 (M+H); found 441.3.

EXAMPLE 41

3-(2,3-Dihydro-benzofuran-5-yl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

a) 3-(2,3-Dihydro-benzofuran-5-yl)-3-oxo-propionic acid ethyl ester

The title compound was synthesized from 1-(2,3-dihydro-benzofuran-5-yl)-ethanone using the procedure described in Example 32, step (a), in 47% yield. ¹H NMR (CDCl₃) 7.85 (d, 1H, J=1.4 Hz), 7.78 (dd, 1H, J=1.9, 8.4 Hz), 6.81 (d, 1H, J=8.4 Hz), 4.67 (t, 2H, J=8.8 Hz), 4.21 (q, 2H, J=7.2 Hz), 3.92 (s, 2H), 3.26 (t, 2H, J=8.7 Hz,), 1.26 (t, 3H, J=7.1 Hz). Mass Spectrum (LCMS, ESI) calculated for Cl₃H₁₅O₄235.1 (M+H); found 235.2.

b) (2,3-Dihydro-benzofuran-5-yl)-propynoic acid ethyl ester

The title compound was synthesized from 3-(2,3-dihydro-benzofuran-5-yl)-3-oxo-propionic acid ethyl ester using the procedure described in Example 32, step (b), in 65% yield. ¹H NMR (CDCl₃) * 7.42-7.38 (m, 2H), 6.77-6.74 (m, 1H), 4.62 (t, 2H, J=8.9H), 4.28 (q, 2H, J=7.2 Hz), 3.21 (t, 2H, J=8.9 Hz), 1.35 (t, 3H, J=7.1 Hz).

c) 7-(2-{1-[1-(2,3-Dihydro-benzofuran-5-yl)-2-ethoxycarbonyl-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from (2,3-dihydro-benzofuran-5-yl)-propynoic acid ethyl ester and 7-[2-(1H-indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (c1), in 52% yield as an E/Z mixture. ¹H NMR (CDCl₃) * 7.31 (d, 1H, J=7.6 Hz), 7.18-7.01 (m, 4H), 6.94 (dd, 1H, J=3.5, 7.6 Hz), 6.82-6.71 (m, 3H), 6.56-6.48 (m, 1H), 6.10 (s, 0.6H), 6.00 (s, 0.4H), 4.62 (q, 2H, J=8.8 Hz), 4.39-4.35 (m, 2H), 4.13 (q, 0.8H, J=7.1 Hz), 3.98 (q, 1.2H. J=7.1 Hz), 3.76 (t, 2H, J=5.9 Hz), 3.23-3.14 (m, 4H), 2.73 (t, 2H, J=6.64 Hz), 1.95-1.89 (m, 2H), 1.52 (s, 9H), 1.21 (t, 1.2H, J=7.1 Hz), 1.00 (t, 1.8 Hz, J=7.1 Hz).
The titled compound is prepared using the procedures described in Example 18, step (e), followed by Example 16, step (e), and Example 18, step (g).

EXAMPLE 42

3-benzo[1,3]dioxol-5-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

a) 5-(2,2-Dibromo-vinyl)-benzo[1,3]dioxole

To a solution of piperonal (4.5 g, 30 mmol) and triphenylphosphine (24 g, 90 mmol) in DCM (120 mL) in an ice-water bath was added a solution of carbontetrabromide (15 g, 45 mmol) over a 10 minutes period. After the addition completed, the ice-water bath was removed, the reaction stirred at ambient temperature for 2 h, and then quenched with saturated NaHCO₃. Aqueous was separated, and extracted with dichloromethane (2 times). The organic layers were combined, dried over Na₂SO₄, filtered, and concentrated to give a redish colored residue, that was filtered through a short path silica gel plug, eluting with DCM/hexane (10% to 20%). Concentration of the filtrate gave the title compound (6.5 g, 74% yield) as a pale yellow liquid. ¹H NMR (CDCl₃) * 7.36 (s, 1H), 7.18 (d, 1H, J=1.6 Hz), 6.95 (dd, 1H, J=1.5, 8.1 Hz), 6.79 (d, 1H, J=8.1 Hz), 5.99 (s, 2H).

b) 5-Ethynyl-benzo[1,3]dioxole

To a solution of 5-(2,2-dibromo-vinyl)-benzo[1,3]dioxole (1.47 g, 5.0 mmol) in THF (10 mL) at −78° C. was added 2.0 M solution of n-butyllithium (5.5 mL, in cyclohexane) over 5 minutes period. After the addition completed, the reaction was stirred for 1 h, and then quenched with saturated NH₄Cl. The mixture was allowed to warm up to room temperature. THF was removed. The aqueous was extracted with ethyl acetate. The organic layer washed with water, brine, dried over Na₂SO₄, concentrated, and flash chromatographed on silica gel, eluting with DCM/hexane (5 to 10%) to give the title compound (0.64 g, 95% yield) as an orange oil. ¹H NMR (CDCl₃) *7.02 (dd, 1H, J=1.6, 8.1 Hz), 6.93 (d, 1H, J=1.6 Hz), 6.75 (d, 1H, J=8.0 Hz), 5.98 (s, 2H), 2.97 (s, 1H).

c) Benzo[1,3]dioxol-5-yl-propynoic acid ethyl ester

The title compound was synthesized from 5-ethynyl-benzo[1,3]dioxole and 7-[2-(1H-indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 23, step (c), in 54% yield. ¹H NMR (CDCl₃) *7.16 (dd, 1H, J=1.6, 8.1 Hz), 7.00 (d, 1H, J=1.6 Hz,), 6.80 (d, 1H, J=8.1 Hz,), 6.02 (s, 2H), 4.29 (q, 2H, J=7.2 Hz,), 1.35 (t, 3H, J=7.2 Hz).

d) 7-{2-[1-(1-Benzo[1,3]dioxol-5-yl-2-ethoxycarbonyl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from benzo[1,3]dioxol-5-yl-propynoic acid ethyl ester and 7-[2-(1H-Indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (c1), in 39% yield as an E/Z mixture. ¹H NMR (CDCl₃) *7.32 (d, 1H, J=7.6 Hz), 7.10-7.04 (m, 2H), 7.00-6.90 (m, 2H), 6.85-6.71 (m, 4H), 6.56-6.49 (m, 1H), 6.10 (s, 0.6H), 6.04 (s, 0.4H), 6.03 (s, 0.8H), 6.00 (s, 1.2H), 4.40-4.35 (m, 2H), 4.13 (q, 0.8H, J=7.1 Hz), 3.98 (q, 1.2H, J=7.1 Hz), 3.76 (t, 2H, J=5.9 Hz), 3.20 (t, 2H, 6.8 Hz), 2.73 (t, 2H, 6.7 Hz), 1.95-1.89 (m, 2H), 1.519 (s, 5.4H), 1.516 (s, 3.6H), 1.22 (t, 1.2H, J=7.1 Hz), 1.01 (t, 1.8H, J=7.1 Hz). Mass Spectrum (LCMS, ESI) calculated for C₃₀H₂₉N₃O₅512.3 (M-Boc+1); found 512.3.

e) 7-{2-[1-(1-Benzo[1,3]dioxol-5-yl-2-ethoxycarbonyl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-{2-[1-(1-Benzo[1,3]dioxol-5-yl-2-ethoxycarbonyl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 18, step (e), in 89% yield as a yellow oil. ¹H NMR (CDCl₃) *7.30 (d, 1H, J=7.6 Hz), 7.21-7.16 (m, 2H), 7.08 (d, 1H, J=2.4 Hz), 6.94 (d, 1H, J=7.6 Hz), 6.82 (dd, 1H, J=2.4, 8.9 Hz), 6.73-6.68 (m, 2H), 6.62 (d, 1H, J=0.7 Hz), 6.42 (d, 1H, J=3.2 Hz), 5.90-5.89 (m, 2H), 4.36 (t, 2H, J=6.9 Hz), 4.04 (q, 2H, J=7.1 Hz), 3.75 (t, 2H, J=6.0 Hz), 3.24-3.15 (m, 4H), 2.72 (t, 2H, J=6.6 Hz), 1.95-1.89 (m, 2H), 1.51 (s, 9H), 1.10 (t, 2H, J=7.1 Hz).

f) 3-Benzo[1,3]dioxol-5-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester

The title compound was synthesized from 7-{2-[1-(1-benzo[1,3]dioxol-5-yl-2-ethoxycarbonyl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (e), in 53% yield as a yellow oil. ¹H NMR (CDCl₃) *7.20-7.15 (m, 2H), 7.09-7.07 (m, 2H), 6.82 (dd, 1H, J=2.4, 9.1 Hz), 6.73-6.68 (m, 2H), 6.62 (bs, 1H), 6.47 (d, 1H, J=7.2 Hz), 6.42 (d, 1H, J=3.0 Hz), 5.92-5.88 (m, 3H), 4.92 (bs, 1H), 4.28 (t, 2H, J=7.0 Hz), 4.04 (q, 2H, J=7.1 Hz), 3.41-3.38 (m, 2H), 3.28-3.15 (m, 2H), 3.03 (t, 2H, J=7.0 Hz), 2.69 (t, 2H, J=6.3 Hz), 1.93-1.87 (m, 2H), 1.10 (t, 3H, J=7.1 Hz).

g) 3-Benzo[1,3]dioxol-5-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

The title compound was synthesized from 3-benzo[1,3]dioxol-5-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester using the procedure described in Example 18, step (g), in 87% yield as a yellow oil. ¹H NMR (CDCl₃) *10.46 (bs, 1H), 7.45 (d, 1H, J=3.2 Hz), 7.26-7.24 (m, 1H), 7.08 (d, 1H, J=7.3 Hz), 6.83 (d, 1H, J=2.3 Hz), 6.69-6.60 (m, 4H), 6.45 (d, 1H, 3.1 Hz), 6.26 (d, 1H, 7.3 Hz), 6.03 (dd, 1H, J=4.6, 11.1 Hz), 5.88-5.86 (m, 2H), 3.65-3.60 (m, 1H), 3.74-3.42 (m, 1H), 3.38-3.35 (m, 2H), 3.28-3.10 (m, 2H), 2.59-2.43 (m, 4H), 1.84-1.80 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₈H₂₈N₃O₅486.2 (M+H); found 486.3.

EXAMPLE 43

3-(5-Methanesulfonyl-pyridin-3-yl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethyoxy]-indol-1-yl}-propionic acid

a) Trimethyl-triethoxyprop-1-ynyl-silane

Boron trifluoride diethyl etherate (36.0 mL, 280 mmol) was added to diethyl ether (50 mL) under argon. The mixture was transferred to a dropping funnel and added dropwise under argon to a solution of tetraethyl orthocarbonate (40.0 g, 208 mmol) in diethyl ether (100 mL) at 0° C. After the addition was complete, the mixture was stirred for 5 min and then cooled to −78° C. In a separate reaction flask, n-butyllithium (166 mL, 2.5 M solution in hexanes, 416 mmol) was added dropwise to a solution of trimethylsilyl acetylene (59.0 mL, 416 mmol) in diethyl ether (200 mL) at 0° C. under argon. After stirring for 1 h at 0° C., the solution was cooled to −78° C. This solution was added via cannula to the triethoxycarbenium tetrafluoroborate formed previously. The mixture was stirred at −78° C. for 1 h before being warming to room temperature. Saturated aqueous potassium carbonate was added and mixture was extracted with diethyl ether. The organic extracts were dried with magnesium sulfate and the solvent was removed under reduced pressure to give the title compound (50.0 g, 100% yield) as yellow oil. ¹H NMR (CDCl₃) * 3.68(q, 6H, J=7.2 Hz), 1.23 (t, 9H, J=7.2 Hz), 0.20, (s, 9H).

b) 3,3,3-Triethoxypropyne

A solution of sodium hydroxide (0.14 g, 3.60 mmol) in water (50 mL) was added to a solution of trimethyl-triethoxyprop-1-ynyl-silane (50.0 g, 208 mmol) in ethanol (250 mL). After stirring for 1 hour at room temperature, water was added and the mixture was extracted with ethyl acetate. The organic extracts were dried with magnesium sulfate and the solvent was removed under reduced pressure to give the title compound (20.0 g, 52% yield) as yellow oil. ¹H NMR (CDCl₃) * 3.70 (q, 6H, J=8.0 Hz), 2.56 (s, 1H) 1.24 (t, 9H, J=8.0 Hz).

c) 3-Bromo-5-methylsulfanyl-pyridine

Sodium thiomethoxide (1.6 g, 23 mmol) was added to a solution of 3,5-dibromopyridine (5 g, 21 mmol) in DMF (25 mL). After stirring overnight at room temperature, the reaction mixture was diluted with ethyl acetate and washed several times with brine. The extracts were dried over magnesium sulfate and the solvent was removed under reduced pressure. The crude material was chromatographed on silica (10% ethyl acetate/hexanes) to give the title compound (3.8 g, 89% yield) as a clear oil.

d) 3-Bromo-5-methanesulfonyl-pyridine

MCPBA (9.2 g, 38 mmol) was added slowly to a solution of 3-bromo-5-methylsulfanyl-pyridine (3.8 g, 19 mmol) in dichloromethane (50 mL). After stirring for 30 minutes, the reaction was diluted with dichloromethane and quenched carefully with 1N NaOH. The product was extracted with dichloromethane and dried over magnesium sulfate. The solvent was removed under reduced pressure to give title compound (2.7 g, 82% yield) as a white solid.

e) 3-Methanesulfonyl-5-triethoxyprop-1-ynyl-pyridine

A solution of 3-bromo-5-methanesulfonyl-pyridine (1.00 g, 4.20 mmol), 3,3,3-triethoxypropyne (1.75 g, 9.4 mmol), dichlorobis(triphenylphosphine)palladium(II) (0.15 g, 0.21 mmol), copper(I) iodide (0.08 g, 0.42 mmol), triethylamine (1.80 mL, 12.7 mmol) and dichloromethane (40 mL) was heated at reflux for 48 h. The mixture was cooled to room temperature and the solvent was removed under reduced pressure. The crude product was chromatographed on silica (30% ethyl acetate/hexanes) to give the title compound (1.2 g, 87% yield) as yellow solid. ¹H NMR (CDCl₃) * 9.08(d, 1H, J=2.4 Hz), 8.91 (d, 1H J=2.0), 8.28 (t, 1H, J=2.0 Hz), 3.75 (q, 6H, J=7.2), 3.13 (s, 3H), 1.29 (t, 9H, J=7.2).

f) (5-Methanesulfonyl-pyridin-3-yl)-propynoic acid ethyl ester

p-Toluenesulfonic acid monohydrate (1.39 g, 0.73 mmol) was added to a solution of 3-methanesulfonyl-5-triethoxyprop-1-ynyl-pyridine (1.20 g, 3.70 mmol) in toluene (40 mL). After stirring overnight at room temperature, the solvent was removed under reduced pressure. The crude product was chromatographed on silica (30% ethyl acetate/hexanes) to give the title compound (0.55 g, 53% yield) as a white solid. ¹H NMR (CDCl₃) * 9.17 (d, 1H, J=2.4 Hz), 9.03 (d, 1H J=2.0), 8.40 (t, 1H, J=2.0 Hz), 4.35 (q, 6H, J=8.0), 3.14 (s, 3H), 1.38 (t, 9H, J=8.0).

g) 7-(2-{1-[2-Ethoxycarbonyl-1-(5-methanesulfonyl-pyridin-3-yl)-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from (5-methanesulfonyl-pyridin-3-yl)-propynoic acid ethyl ester and 7-[2-(1H-indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (d1), in 80% yield. Mass Spectrum (LCMS, ESI) calculated for C₂₉H₃₁N₄O₅S: 547.2 (M-Boc+H); found 547.3 (-Boc).

h) 7-(2-{1-[2-Ethoxycarbonyl-1-(5-methanesulfonyl-pyridin-3-yl)-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihyrdro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-(2-{1-[2-ethoxycarbonyl-1-(5-methanesulfonyl-pyridin-3-yl)-vinyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 18, step (e), in 25% yield. ¹H NMR (CDCl₃) * 9.03 (d, 1H, J=2.0 Hz), 8.71 (d, 1H, J=2.0 Hz), 7.97 (t, 1H, J=2.0 Hz), 7.30 (m, 2H), 7.20 (d, 1H, J=3.6 Hz), 6.84 (dd, 1H, J=1.6, 6.8 Hz), 6.93 (m, 2H), 6.52 (d, 1H, J=3.2 Hz), 6.12 (t, 1H, J=3.2 Hz).

i) 3-(5-Methanesulfonyl-pyridin-3-yl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}propionic acid ethyl ester

The title compound was synthesized from 7-(2-{1-[2-ethoxycarbonyl-1-(5-methanesulfonyl-pyridin-3-yl)-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihyrdro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (f), in 32% yield. ¹H NMR (CD₃OD) * 8.96 (s, 1H), 8.67 (s, 1H), 8.16 (s, 1H), 7.96 (s, 1H), 7.49 (d, 1H, J=2.8 Hz), 7.43 (d, 1H, J=7.2 Hz), 7.29 (d, 1H, J=8.8), 7.06 (d, 1H, J=2.0 Hz), 6.76 (dd, 1H, J=2.4, 6.4 Hz), 6.63 (d, 1H, J=7.6 Hz), 6.48 (d, 1H, J=2.8 Hz), 6.40 (t, 1H, J=3.2 Hz), 4.24 (t, 2H, J=6.0 Hz), 4.01 (m, 2H), 3.51 (m, 2H), 3.42 (t, 2H, J=5.6 Hz), 3.06 (t, 2H, J=6.0 Hz), 2.97 (s, 3H), 2.74 (t, 2H, J=6.0 Hz), 1.99 (m, 2H), 1.05 (t, 3H, J=6.8 Hz).

j) Methanesulfonyl-pyridin-3-yl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

The title compound was synthesized from 3-(5-Methanesulfonyl-pyridin-3-yl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}propionic acid ethyl ester using the procedure described in Example 18, step (g), in 30% yield. ¹H NMR (CDCl₃) *10.50 (s, 1H), 8.91 (s, 1H), 8.61 (s, 1H), 7.89 (s, 1H), 7.37 (d, 1H, J=2.8 Hz), 7.10 (d, 1H, J=7.6 Hz), 7.01 (d, 1H, J=8.8 Hz), 6.83 (d, 1H, J=1.6 Hz), 6.55 (d, 1H, J=8.8 Hz), 6.43 (d, 1H, J=2.8 Hz), 6.25 (d, 1H, J=7.6 Hz), 6.14 (t, 1H, J=7.2 Hz), 3.74 (m, 2H), 3.34 (m, 2H), 3.23 (m, 2H), 3.10 (m, 2H), 2.93 (s, 3H), 2.59 (t, 2H, J=6.0 Hz), 1.78 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₇H₂₉N₄O₃S: 521.2 (M+H); found 521.3.

EXAMPLE 44

3-{5-[2-(6-Methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-3-phenyl-propionic acid

a) [6-(2-Hydroxy-ethyl)-pyridin-2-yl]-methyl-carbamic acid tert-butyl ester

The title compound was synthesized from [6-(tert-butoxycarbonyl-methyl-amino)-pyridin-2-yl]-acetic acid ethyl ester (synthetic methodology described in WO 98/14192) using the procedure described in Example 16, step (a), in 80% yield. ¹H NMR (Cl₃CD), δ: 7.55 (m, 2H), 6.85 (dd, 1H, J=1.1, 6.7 Hz), 4.00 (m, 2H), 3.37 (s, 3H), 2.97 (m, 2H), 1.53 (s, 9H).

b) Methyl-{6-[2-(3-methyl-4-nitro-phenoxy)-ethyl]-pyridin-2-yl}-carbamic acid tert-butyl ester

The title compound was synthesized from [6-(2-hydroxy-ethyl)-pyridin-2-yl]-methyl-carbamic acid tert-butyl ester and the commercially available 3-methyl-4-nitro-phenol using the procedure described in Example 16, step (b), in 81% yield. ¹H NMR (Cl₃CD), δ: 1.52 (s, 9H), 2.62 (s, 3H), 3.21 (t, 2H, J=8.00 Hz), 3.36 (s, 3H), 4.44 (t, 2H, J=8.00 Hz), 6.80 (m, 2H), 6.94 (dd, 1H, J=2.4, 5.6 Hz), 7.55 (m, 2H), 8.05 (d, 1H, J=8.8 Hz).

c) {6-[2-(1H-Indol-5-yloxy)-ethyl]-pyridin-2-yl}-methyl-carbamic acid tert-butyl ester

The title compound was synthesized from methyl-{6-[2-(3-methyl-4-nitro-phenoxy)-ethyl]-pyridin-2-yl}-carbamic acid tert-butyl ester using the procedure described in Example 16, step (c), in 48% yield. H¹NMR (Cl₃CD), δ: 8.09 (1H, br s), 7.55 (m, 1H), 7.49 (d, 1H, J=7.8 Hz), 7.26 (d, 1H, J=8.7 Hz), 7.17 (m, 1H), 7.14 (d, 1H, J=2.4 Hz), 6.98 (d, 1H, J=7.3 Hz), 6.85 (dd, 1H, J=2.4, 6.8 Hz), 6.80 (m, 2H), 6.46 (m, 1H), 4.39 (t, 2H, J=6.8 Hz), 3.39 (s, 3H), 3.22 (t, 2H, J=6.8 Hz), 1.51 (s, 9H).

d) 3-(5-{2-[6-(tert-Butoxycarbonyl-methyl-amino)-pyridin-2-yl]-ethoxy}-indol-1-yl)-3-phenyl-acrylic acid ethyl ester

The title compound was synthesized from {6-[2-(1H-indol-5-yloxy)-ethyl]-pyridin-2-yl}-methyl-carbamic acid tert-butyl ester and the commercially available phenyl propynoic acid ethyl ester using the procedure described in Example 16, step (dl), in 81% yield as an E/Z isomeric mixture. H¹NMR (Cl₃CD), δ: 7.57-7.53 (m, 1H), 7.52-7.46 (m, 1.5H), 7.44 (m, 1H), 7.41-7.34 (m, 2.5H), 7.29 (m, 1H), 7.12 (d, 0.5H, J=2.1 Hz), 7.07 (m, 1.5H), 6.97 (m, 1.5H), 6.76 (m, 1H), 6.70 (m, 0.5H), 6.59 (d, 0.5H, J=3.2 Hz), 6.51 (d, 0.5H, J=3.5 Hz), 6.22 (s, 0.5H), 6.15 (s, 0.5H), 4.38 (m, 2H), 4.09 (c, 1.5H, J=7.0 Hz), 4.01 (c, 1.5H, J=7.2 Hz), 3.39 (m, 3H), 3.21 (m, 2H), 1.52 (s, 9H), 1.16 (t, 1.5H, J=7.2 Hz), 1.03 (t, 1.5H, J=7.0 Hz).

e) 3-(5-{2-[6-(tert-Butoxycarbonyl-methyl-amino)-pyridin-2-yl]-ethoxy}-indol-1-yl)-3-phenyl-propionic acid ethyl ester

The title compound was synthesized from 3-(5-{2-[6-(tert-butoxycarbonyl-methyl-amino)-pyridin-2-yl]-ethoxy}-indol-1-yl)-3-phenyl-acrylic acid ethyl ester using the procedure described in Example 16, step (e), in 97% yield. H¹NMR (Cl₃CD), δ: 7.54 (m, 1H) 7.48 (d, 1H, J=7.2 Hz), 7.29-7.16 (m, 7H), 7.10 (d, 1H, J=2.4 Hz), 6.97 (dd, 1H, J=0.8, 7.2 Hz), 6.80 (dd, 1H, J=2.4, 9.2 Hz), 6.45 (dd, 1H, J=0.8, 3.6 Hz), 6.02 (t, 1H, J=7.6 Hz), 4.37 (t, 2H, J=8.0 Hz), 4.04 (c, 2H, J=8.0 Hz), 3.38 (s, 3H), 3.27 (m, 2H), 3.20 (t, 2H, J=8.0 Hz), 1.51 (s, 9H), 1.10 (t, 3H, J=8.0 Hz).

f) 3-{5-[2-(6-Methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-3-phenyl-propionic acid ethyl ester

The title compound was synthesized from 3-(5-{2-[6-(tert-butoxycarbonyl-methyl-amino)-pyridin-2-yl]-ethoxy}-indol-1-yl)-3-phenyl-propionic acid ethyl ester using the procedure described in Example 16, step (f), in 73% yield. H¹NMR (Cl₃CD), δ: 7.40 (m, 1H) 7.30-7.25 (m, 3H), 7.20-7.16 (m, 4H), 7.10 (d, 1H, J=2.4 Hz), 6.81 (dd, 1H, J=2.4, 8.9 Hz), 6.56 (d, 1H, J=7.2 Hz), 6.44 (d, 1H, J=3.2 Hz), 6.24 (d, 1H, J=8.2 Hz), 6.01 (t, 1H, J=7.6 Hz), 4.55 (br s, 1H), 4.32 (t, 2H, J=8.0 Hz), 4.03 (c, 2H, J=8.0 Hz), 3.26 (m, 1H), 3.09 (t, 3H, J=8.0 Hz), 2.89 (s, 3H), 1.08 (t, 3H, J=8.0 Hz).

g) 3-{5-[2-(6-Methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-3-phenyl-propionic acid

The title compound was synthesized from 3-{5-[2-(6-methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-3-phenyl-propionic acid ethyl ester using the procedure described in Example 16, step (g), in 66% yield. H¹NMR (DMSO-d6), δ: 7.66 (m, 1H). 7.39-7.19 (m, 7H), 7.04 (d, 1H, J=2.4 Hz), 6.70 (dd, 1H, J=2.4, 9.2 Hz), 6.43 (d, 1H, J=6.8 Hz), 6.35 (m, 2H), 6.25 (d, 1H, J=8.0 Hz), 5.94 (m, 1H), 4.25 (t, 2H, J=8.0 Hz), 3.39 (m, 1H), 2.94 (t, 3H, J=8.0 Hz), 2.74 (m, 3H). Mass Spectrum (LCMS, ESI) calculated for C₂₅H₂₆N₃O₃416.2, (M+1); found: 416.3.

EXAMPLE 45

3-{5-[2-(6-Methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-3-quinolin-3-yl-propionic acid

a) 3-Ethynyl-quinoline

The title compound was synthesized from the commercially available 3-bromo quinoline using the procedures described in Example 18, step (a) and step (b), in 68% yield. H¹NMR (Cl₃CD), δ: 8.95 (d, 1H, J=2.0 Hz), 8.29 (d, 1H, J=2.0 Hz), 8.09 (d, 1H, J=8.8 Hz), 7.80 (m, 1H), 7.74 (m, 1H), 7.60 (m, 1H), 3.28 (s, 1H).

b) Quinolin-3-yl-propynoic acid ethyl ester

The title compound was synthesized from 3-ethynyl-quinoline using the procedure described in Example 23, step (c), in 34% yield. H¹NMR (Cl₃CD), δ: 8.99 (d, 1H, J=2.0 Hz), 8.40 (d, 1H, J=2.0 Hz), 8.11 (d, 1H, J=8.4 Hz), 7.80 (m, 2H), 7.60 (m, 1H), 4.34 (q, 2H, J=7.2 Hz), 1.38 (t, 3H, J=7.2 Hz).

c) 3-(5-{2-[6-(tert-Butoxycarbonyl-methyl-amino)-pyridin-2-yl]-ethoxy}-indol-1-yl)-3-quinolin-3-yl-acrylic acid ethyl ester

The title compound was synthesized from {6-[2-(1H-indol-5-yloxy)-ethyl]-pyridin-2-yl}-methyl-carbamic acid tert-butyl ester and quinolin-3-yl-propynoic acid ethyl ester, using the procedure described in Example 16, step (d1), in 48% yield, as an E/Z isomeric mixture. H¹NMR (Cl₃CD), δ: 8.91 (d, 0.3H, J=2.1 Hz), 8.88 (d, 0.3H, J=2.3 Hz), 8.17 (d, 0.7H, J=8.8 Hz), 8.14-8.11 (m, 1H), 7.97 (d, 0.3H, J=2.0 Hz), 7.82-7.74 (m, 2H), 7.60 (m, 1H), 7.53 (m, 1H), 7.49 (m, 1H), 7.17 (m, 1H), 7.11 (m, 1H), 6.92-6.97 (m, 2H), 6.78 (dd, 0.7H, J=2.5, 9.0 Hz), 6.70 (dd, 0.3H, J=2.5, 9.0 Hz), 6.64 (d, 0.3H, J=3.2 Hz), 6.55 (d, 0.7H, J=3.5 Hz), 6.39 (s, 0.3H), 6.32 (s, 0.7H), 4.39 (m, 2H), 4.10 (q, 1.4H, J=7.2 Hz), 4.04 (q, 0.6H, J=7.2 Hz), 1.39 (s, 0.9H), 1.38 (s, 2.1H), 3.21 (m, 2H), 1.51 (s, 9H), 1.14 (t, 2.1H, J=7.2 Hz), 1.05 (t, 0.9H, J=7.2 Hz).

d) 3-(5-{2-[6-(tert-Butoxycarbonyl-methyl-amino)-pyridin-2-yl]-ethoxy}-indol-1-yl)-3-quinolin-3-yl-propionic acid ethyl ester

The title compound was synthesized from 3-(5-{2-[6-(tert-butoxycarbonyl-methyl-amino)-pyridin-2-yl]-ethoxy}-indol-1-yl)-3-quinolin-3-yl-acrylic acid ethyl ester using the procedure described in Example 18, step (d), in 53% yield. H¹NMR (Cl₃CD), δ: 8.88 (m, 1H), 8.06 (d, 1H, J=8.6 Hz), 7.87 (m, 1H), 7.72 (m, 1H), 7.68 (m, 2H), 7.55-7.47 (m, 3H), 7.24 (m, 2H), 7.11 (d, 1H, J=2.3 Hz), 6.95 (d, 1H, J=7.2 Hz), 6.81 (dd, 1H, J=2.3, 8.8 Hz), 6.51 (d, 1H, J=3.2 Hz), 6.23 (t, 1H, J=7.4 Hz), 4.36 (t, 2H, J=6.7 Hz), 4.07 (q, 2H, J=7.2 Hz), 3.38 (m, 5H), 3.19 (t, 2H, J=6.7 Hz), 1.51 (s, 9H), 1.11 (t, 3H, J=7.2 Hz).

e) 3-{5-[2-(6-Methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-3-quinolin-3-yl-propionic acid ethyl ester

The title compound was synthesized from 3-(5-{2-[6-(tert-butoxycarbonyl-methyl-amino)-pyridin-2-yl]-ethoxy}-indol-1-yl)-3-quinolin-3-yl-propionic acid ethyl ester using the procedure described in Example 16, step (f), in 20% yield. H¹NMR (Cl₃CD), δ: 8.83 (d, 1H, J=2.3 Hz), 8.08 (d, 1H, J=8.4 Hz), 7.89 (m, 1H), 7.75 (m, 1H), 7.71 (m, 1H), 7.55 (m, 1H), 7.40 (m, 1H), 7.24 (d, 1H, J=3.2 Hz), 7.23 (m, 1H), 7.14 (d, 1H, J=2.3 Hz), 6.84 (dd, 1H, J=2.5, 9.0 Hz), 6.57 (d, 1H, J=7.2 Hz), 6.52 (d, 1H, J=3.2 Hz), 6.25 (m, 2H), 4.52 (br s, 1H), 4.35 (t, 2H, J=6.9 Hz), 4.10 (q, 2H, J=7.2 Hz), 3.43 (m, 2H), 3.10 (t, 2H, J=6.9 Hz), 2.91 (m, 3H), 1.14 (t, 3H, J=7.2 Hz).

f) 3-{5-[2-(6-Methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-3-quinolin-3-yl-propionic acid

The title compound was synthesized from 3-{5-[2-(6-methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-3-quinolin-3-yl-propionic acid ethyl ester using the procedure described in Example 18, step (g), in 60% yield. H¹NMR (DMSO-d6) δ: 8.91 (d, 1H, J=2.3 Hz), 8.33 (d, 1H, J=2.0 Hz), 7.96 (d, 1H, J=8.6 Hz), 7.90 (m, 1H), 7.77 (d, 1H, J=3.2 Hz), 7.59 (m, 1H), 7.72 (m, 1H), 7.52 (d, 1H, J=9.0 Hz), 7.28 (m, 1H), 7.06 (d, 1H, J=2.5 Hz), 6.71 (dd, 1H, J=2.3, 8.8 Hz), 6.43 (m, 2H), 6.33(m, 1H), 6.25 (d, 1H, J=8.6 Hz), 6.21 (m, 1H), 4.25 (t, 2H, J=6.7 Hz), 3.56 (m, 2H), 2.93 (t, 2H, J=6.7 Hz), 2.73 (m, 3H). Mass Spectrum (LCMS, ESI) calculated for C₂₈H₂₇N₄O₃467.2, (M+1); found: 467.2.

EXAMPLE 46

3-{5-[2-(6-methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-3-pyridin-3-yl-propionic acid

a) 3-(5-{2-[6-(tert-Butoxycarbonyl-methyl-amino)-pyridin-2-yl]-ethoxy}-indol-1-yl)-3-pyridin-3-yl-acrylic acid methyl ester

The title compound was synthesized from {6-[2-(1H-indol-5-yloxy)-ethyl]-pyridin-2-yl}-methyl-carbamic acid tert-butyl ester and pyridin-3-yl-propynoic acid methyl ester, using the procedure described in Example 16, step (d1), in a 96% yield, as an E/Z isomeric mixture. ¹H NMR (Cl₃CD), δ: 8.74-8.64 (m, 2H), 7.68 (m, 0.4), 7.57-7.48 (m, 2H), 7.45 (m, 0.6H), 7.38 (m, 0.4H), 7.28 (m, 0.6H), 7.13 (m, 1H), 7.08 (m, 1H), 6.96 (d, 1H, J=7.0 Hz), 6.88 (d, 0.4H, J=3.5 Hz), 6.80-6.66 (m, 1.6H), 6.61 (d, 0.6H, J=3.2 Hz), 6.54 (d, 0.4H, J=3.5 Hz), 6.26 (s, 0.4H), 6.24 (s, 0.6H), 4.39 (m, 2H), 3.67 (s, 1.2H), 3.61 (s, 1.8H), 3.39 (m, 3H), 3.20 (m, 2H), 1.51 (s, 9H).

b) 3-(5-{2-[6-(tert-Butoxycarbonyl-methyl-amino)-pyridin-2-yl]-ethoxy}-indol-1-yl)-3-pyridin-3-yl-propionic acid methyl ester

The title compound was synthesized from 3-(5-{2-[6-(tert-butoxycarbonyl-methyl-amino)-pyridin-2-yl]-ethoxy}-indol-1-yl)-3-pyridin-3-yl-acrylic acid methyl ester using the procedure described in Example 16, step (e), in 53% yield. ¹H NMR (Cl₃CD), δ: 8.55 (d, 1H, J=2.3 Hz), 8.51 (dd, 1H, J=1.6, 8.5 Hz), 7.52 (m, 2H), 7.21-7.15 (m, 3H), 7.37 (m, 1H), 7.10 (d, 1H, J=2.3 Hz), 6.96 (dd, 1H, J=0.6, 7.1 Hz), 6.81 (dd, 1H, J=2.4, 8.9 Hz), 6.48 (d, 1H, J=3.1 Hz), 6.04 (t, 1H, J=7.6 Hz), 4.36 (t, 2H, J=8.0 Hz), 3.62 (s, 3H), 3.38 (s, 3H), 3.31 (m, 2H), 3.19 (t, 2H, J=8.0 Hz), 1.51 (s, 9H).

c) 3-{5-[2-(6-Methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-3-pyridin-3-yl-propionic acid methyl ester

The title compound was synthesized from 3-(5-{2-[6-(tert-butoxycarbonyl-methyl-amino)-pyridin-2-yl]-ethoxy}-indol-1-yl)-3-pyridin-3-yl-propionic acid methyl ester using the procedure described in Example 16, step (f), in 55% yield. ¹H NMR (Cl₃CD), δ: 8.55 (d, 1H, J=2.3 Hz), 8.51 (dd, 1H, J=1.5, 4.8 Hz), 7.37 (m, 2H), 7.18 (m, 3H), 7.10 (d, 1H, J=2.4 Hz), 6.82 (dd, 1H, J=2.4, 8.9 Hz), 6.55 (d, 1H, J=7.2 Hz), 6.47 (d, 1H, J=2.9 Hz), 6.23 (d, 1H, J=8.2 Hz), 6.04 (t, 1H, J=7.5 Hz), 4.54 (br s, 1H), 4.33 (t, 2H, J=8.0 Hz), 3.62 (s, 3H), 3.31 (m, 2H), 3.09 (t, 2H, J=8.0 Hz), 2.89 (m, 3H).

d) 3-{5-[2-(6-Methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-3-pyridin-3-yl-propionic acid

The title compound was synthesized from 3-{5-[2-(6-methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-3-pyridin-3-yl-propionic acid methyl ester using the procedure described in Example 16, step (g), in 42% yield. ¹H NMR (DMSO-d6) δ: 8.62 (br s, 1H), 8.44 (br s, 1H), 7.71 (m, 2H), 7.46 (d, 1H, J=8.9 Hz), 7.29 (m, 2H), 7.05 (d, 1H, J=2.3 Hz), 6.71 (dd, 1H, J=2.3, 8.9 Hz), 6.43 (d, 1H, J=7.1 Hz), 6.39 (d, 1H, J=3.1 Hz) 6.35 (m, 1H), 6.25 (d, 1H, J=8.2 Hz), 4.25 (t, 2H, J=8.0 Hz), 6.02 (m, 1H), 3.49 (m, 2H), 2.93 (t, 2H, J=8.0 Hz), 2.74 (m, 3H). Mass Spectrum (LCMS, ESI) calculated for C₂₅H₂₅N₄O₃417.2, (M+1); found: 417.3.

EXAMPLE 47

3-{5-[2-(6-Methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-hexanoic acid

a) 3-{5-[2-(6-Methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-hexanoic acid ethyl ester

The title compound was synthesized from 2-(6-methylamino-pyridin-2-yl)-ethanol and 3-(5-hydroxy-indol-1-yl)-hexanoic acid ethyl ester using the procedure described in Example 14, step (c), in 25% yield. The crude product was used in the next step without further purification.

b) 3-{5-[2-(6-Methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-hexanoic acid

The title compound was synthesized from 3-{5-[2-(6-methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-hexanoic acid ethyl ester using the procedure described in Example 14, step (e), in 51% yield. ¹H NMR (CDCl₃) δ 7.50 (dd, 1H, J=7.4, 8.8 Hz), 7.38 (d, 1H, J=9.0 Hz), 7.21 (d, 1H, J=3.2 Hz), 6.91 (d, 1H, J=2.4 Hz), 6.69 (dd, 1H, J=2.4, 8.9 Hz), 6.49 (d, 1H, J=7.3 Hz), 6.43 (d, 1H, J=3.1 Hz), 6.33 (d, 1H, J=8.7 Hz), 4.92-2.84 (m, 1H), 3.93-3.88 (m, 1H), 3.79-3.75 (m, 1H), 2.92-2.66 (m, 7H), 1.87-1.77 (m, 2H), 1.26-1.14 (m, 1H), 1.13-1.01 (m, 1H), 0.84 (t, 3H, J=7.2 Hz). Mass spectrum (LCMS, ESI) calculated for C₂₂H₂₈N₃O₃382.2 (M+H); found 382.3.

EXAMPLE 48

3-{5-[2-(2-Methyl-5,6,7,8-tetrahydro-[1,8]naphthyridin-3-yl)-ethyl]-indol-1-yl}-propionic acid

a) 4-(1H-Indol-5-yl)-butyronitrile

A mixture of 5-bromoindole (0.25 g, 1.25 mmol) and tetrakis(triphenylphosphine)palladium(0) (0.10 g, 0.12 mmol) was stirred under a nitrogen atmosphere for 10 minutes. 3-cyanopropyl zinc bromide [0.5 M in THF] (5.0 mL, 2.50 mmol) was added to the mixture and heated in the microwave at 100° C. for 15 minutes. The solvent was removed and the crude mixture was purified via column chromatography with silica gel, eluting with hexane/ethyl acetate (4/1) to afford the title compound in 63% yield. ¹H NMR (CDCl₃) δ 8.22 (br, 1H), 7.43 (s, 1H), 7.31 (d, J=8.3 Hz, 1H), 7.18 (s, 1H), 7.00 (d, J=8.3 Hz, 1H), 6.49 (s, 1H), 2.86 (t, J=7.3 Hz, 2H), 2.28 (t, J=7.2 Hz, 2H), 2.02 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₁₂H₁₃N₂185.1 (M+H); found 185.1.

b) 5-Bromo-1-triisopropylsilanyl-1H-indole

Lithium hexamethyldisilazane [1.0 M] (44.7 mL, 44.4 mmol) was added to a solution of 5-bromo-1H-indole (7.30 g, 37.0 mmol) in tetrahydrofuran (50 mL) at room temperature. After stirring for 5 minutes, triisopropylsilyl chloride (8.62 g, 44.4 mmol) was added to reaction mixture and stirred for 30 minutes. Water was added to quench the reaction and the solvent was removed under reduced pressure to give the crude mixture, which was purified via column chromatography on silica gel (9:1 hexane/ethyl acetate) to give the title compound in 92% yield. ¹H NMR (CDCl₃) δ 7.74 (d, J=1.8, 1H), 7.36 (d, J=8.8 Hz, 1H), 7.22 (m, 2H), 6.55 (d, J=3.1 Hz, 1H), 1.65 (m, 3H), 1.12 (d, J=7.5 Hz, 18H).

c) 4-(1-Triisopropylsilanyl-1H-indol-5-yl)-butyronitrile

Method c1
A mixture of 5-bromo-1-triisopropylsilanyl-1H-indole (4.30 g, 12.2 mmol), tetrakis(triphenylphosphine)-palladium (0) (1.41 g, 1.22 mmol), and 3-cyanopropyl zinc bromide [0.5 M in THF] (50 mL, 24.4 mmol) was heated at 70° C. overnight. The reaction was cooled and 1.0 N HCl (50 mL) was added. The crude product was extracted with methylene chloride (3×30 mL), and the combined organic layers were washed with water, brine, and then dried over Na₂SO₄. Removal of solvent gave a crude mixture which was purified via column chromatography, eluting with hexane/ethyl acetate (9/1) to give the title compound (64% yield).
Method c2
Lithium hexamethyldisilazane [1.0 M] (0.90 mL, 0.90 mmol) was added dropwise to a solution of 4-(1H-indol-5-yl)-butyronitrile (0.15 g, 0.82 mmol) in THF (2 mL) at −78° C. under nitrogen. After 5 minutes, triisopropylsilyl chloride (0.40 mL, 0.90 mmol) was added and the reaction was warmed to room temperature and stirred for an additional 4 h. Water was added to quench the reaction and the solvent was removed under reduced pressure. The crude mixture was purified via column chromatography with silica gel, eluting with hexane/ethyl acetate (4/1) to give the title compound (95% yield). ¹H NMR (CDCl₃) δ 7.45 (d, 2H), 7.25 (m, 1H), 6.90 (m, 1H), 6.55 (m, 1H), 2.86 (t, 2H), 2.30 (t, 2H), 2.06 (m, 2H), 1.67 (m, 3H), 1.10 (d, 18H).

d) 5-(1-Triisopropylsilanyl-1H-indol-5-yl)-pentan-2-one

Methyl magnesium iodide [3 M in ether] (12.0 mL, 36.0 mmol) was added to a solution of 4-(1H-indol-5-yl)-butyronitrile (6.14 g, 18.0 mmol) in ether (50 mL) at 78° C. After addition, the reaction mixture was warmed to room temperature and stirred for 2 days. The reaction was quenched with a saturated ammonium chloride and the crude product was extracted with dichloromethane. The solvent was removed under reduced pressure and the crude mixture was purified via column chromatography with silica gel, eluting with hexane/ethyl acetate (4/1) to give the title compound (86% yield). ¹H NMR (CDCl₃) δ 7.41 (m, 2H), 7.21 (m, 1H), 6.95 (dd, J=1.9, 8.5 Hz, 1H), 6.55 (dd, J=0.6, 2.2 Hz, 1H), 2.69 (t, J=7.5 Hz, 2H), 2.43 (t, J=7.4 Hz, 2H), 2.09 (s, 3H), 1.96 (m, 2H), 1.71 (, 3H), 1.13 (d, J=7.6 Hz, 9H). ¹³C NMR (CDCl₃) δ 209.1, 139.3, 132.6, 131.5, 131.3, 122.2, 119.8, 113.6, 104.3, 42.9, 34.9, 29.6, 29.9, 25.7, 18.1, 12.7.

e) 2-[3-(1-Triisopropylsilanyl-1H-indol-5-yl)-propyl]-[1,8]naphthyridine and 2-Methyl-3-[2-(1-triisopropylsilanyl-1H-indol-5-yl)-ethyl]-[1,8]naphthyridine

A mixture of 5-(1-triisopropylsilanyl-1H-indol-5-yl)-pentan-2-one (1.10 g, 3.07 mmol), 2-amino-pyridine-3-carbaldehyde (0.37 g, 3.07 mmol), and L-proline (0.18 g, 1.53 mmol) in ethanol (15 mL) was heated at reflux for 24 h. The solvent was removed under reduced pressure to give a crude mixture which was purified via column chromatography, eluting with hexane/ethyl acetate (1/2) to give the two title compounds in a 2:1 ratio.
2-[3-(1-Triisopropylsilanyl-1H-indol-5-yl)-propyl]-[1,8]naphthyridine (major isomer, 56% yield): ¹H NMR (CDCl₃) δ 9.08 (dd, J=2.0, 4.3 Hz, 1H), 8.13 (dd, J=1.9, 8.0 Hz, 1H), 8.06 (d, J=8.3 Hz, 1H), 7.37-7.45 (m, 4H), 7.21 (d, J=3.2 Hz, 1H), 7.01 (dd, J=1.8 and 8.5, 1H), 6.54 (dd, J=0.6, 2.4 Hz, 1H), 3.11 (t, J=7.7 Hz, 2H), 2.83 (t, J=7.6 Hz, 2H), 2.28 (m, 2H), 1.69 (m, 3H), 1.13 (d, J=7.5 Hz, 18H). ¹³C NMR (CDCl₃) δ 166.6, 155.8, 152.9, 139.2, 136.7, 136.5, 133.1, 131.5, 131.1, 122.4, 122.3, 121.1, 120.8, 119.7, 113.5, 104.3, 38.8, 35.5, 31.4, 17.9, 12.6. Mass Spectrum (LCMS, ESI) calculated for C₂₈H₃₈N_3Si444.3 (M+H); found 444.4.
2-Methyl-3-[2-(1-triisopropylsilanyl-1H-indol-5-yl)-ethyl]-[1,8]naphthyridine (minor isomer, 24% yield): ¹H NMR (CDCl₃) δ 9.01 (dd, J=1.9, 3.2 Hz, 1H), 8.04 (dd, J=1.9, 8.1 Hz, 1H), 7.80 (s, 1H), 7.37-7.47 (m, 4H), 6.95 (dd, J=1.6, 8.4 Hz, 1H), 6.56 (d, J=3.1 Hz, 1H), 3.07-3.18 (m, 4H), 2.81 (s, 3H), 1.57-1.73 (m, 3H), 1.14 (d, J=7.6 Hz, 18H). ¹³C NMR (CDCl₃) δ 162.4, 154.5, 152.2, 139.4, 135.9, 135.1, 135.0, 131.9, 131.5, 131.4, 122.0, 121.3, 121.1, 119.6, 113.6, 104.2, 35.7, 35.1, 23.5, 17.9, 12.6. Mass Spectrum (LCMS, ESI) calculated for C₂₈H₃₈N_3Si444.23 (M+H); found 444.4.

f) 3-{5-[2-(2-Methyl-[1,8]naphthyridin-3-yl)-ethyl]-indol-1-yl}-acrylic acid methyl ester

A mixture of 2-methyl-3-[2-(1-triisopropylsilanyl-1H-indol-5-yl)-ethyl]-[1,8]naphthyridine (0.25 g, 0.74 mmol), propynoic acid methyl ester (0.07 g, 0.84 mmol), and tetrabutylammonium fluoride [1.0 M] (2.23 mL, 2.23 mmol) was stirred at room temperature overnight. The solvent was removed under reduced pressure to give a crude mixture which was purified via column chromatography with silica gel, eluting with methylene chloride/methanol (95/5) to give the title compound (66% yield) as an E/Z isomeric mixture. ¹H NMR (CDCl₃) δ 9.00 (m, 1H), 8.23 (s, 1H), 8.08 (m, 1H), 7.80 (m, 1H), 7.50 (d, 1H). Mass Spectrum (LCMS, ESI) calculated for C₂₂H₂₂N₃O₂372.2 (M+H); found 372.2.

g) 3-{5-[2-(2-Methyl-5,6,7,8-tetrahydro-[1,8]naphthyridin-3-yl)-ethyl]-indol-1-yl}-propionic acid methyl ester

3-{5-[2-(2-Methyl-[1,8]naphthyridin-3-yl)-ethyl]-indol-1-yl}-acrylic acid methyl ester (230 mg, 6.1 mmol) was stirred in methanol (5 mL) under a hydrogen atmosphere in the presence of 10% palladium on carbon (10% w/w) (20 mg) for 3 days. After removal of solvent, the crude product was purified by flash chromatography on silica gel with methylene chloride/methanol (95/5) to give the title product (14 mg, 6% yield). ¹H NMR (CDCl₃) δ 7.40 (1H), 7.33 (1H), 7.23 (1H), 7.10 (1H), 7.00 (d, 1H), 6.65 (br, 1H), 6.45 (1H), 4.45 (t, 2H), 3.67 (s, 3H), 3.40 (br, 2H), 2.85 (4H), 2.30 (s, 3H), 2.70 (m, 2H), 1.90 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₃H₂₈N₃O₂378.2 (M+H); found 378.3.

h) 3-{5-[2-(2-Methyl-5,6,7,8-tetrahydro-[1,8]naphthyridin-3-yl)-ethyl]-indol-1-yl}-propionic acid

The title compound was synthesized from 3-{5-[2-(2-methyl-5,6,7,8-tetrahydro-[1,8]naphthyridin-3-yl)-ethyl]-indol-1-yl}-propionic acid methyl ester using the procedure described in Example 14, step (e), in 56% yield. ¹H NMR (CDCl₃) δ 7.34 (d, J=8.2 Hz, 1H), 7.25 (m, 1H), 7.20 (m, 2H), 6.86 (m, 1H), 6.28 (d, J=2.8 Hz, 1H), 4.43 (t, J=7.0 Hz, 2H), 3.39 (m, 2H), 2.85 (t, J=3.8 Hz, 2H), 2.79 (t, J=6.7 Hz, 2H), 2.68 (m, 4H), 1.97 (s, 3H), 1.88 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₂H₂₆N₃O₂364.2 (M+H); found 364.3.

EXAMPLE 49

3-{5-[3-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-propyl]-indol-1-yl}-propionic acid

a) 2-[3-(1H-Indol-5-yl)-propyl]-[1,8]naphthyridine

Tetrabutylammonium fluoride [1.0 M in THF] (5.10 mL, 5.10 mmol) was added to a solution of 2-[3-(1-triisopropylsilanyl-1H-indol-5-yl)-propyl]-[1,8]naphthyridine (1.14 g, 2.57 mmol) in THF (20 mL) at room temperature and stirred for 1 h. The solvent was removed and the resulting crude product was purified via column chromatography on silica gel, eluting with ethyl acetate/hexane (2/1) to give the title product (100% yield). ¹H NMR (CDCl₃) δ 9.08 (dd, J=2.0, 4.3 Hz, 1H), 8.28 (br, 1H), 8.14 (dd, J=2.0, 8.1, 1H), 8.07 (d, J=8.4 Hz, 1H), 7.45 (m, 2H), 7.36 (d, J=8.3, 1H), 731 (d, J=8.3 Hz, 1H), 7.18 (t, J=2.8 Hz, 1H), 7.05 (dd, J=1.6, 8.3 Hz, 1H), 6.47 (m, 1H), 3.10 (t, J=7.8 Hz, 2H), 2.84 (t, J=7.7 Hz, 2H), 2.28 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₁₉H₁₈N₃288.2 (M+H); found 288.2.

b) 3-[5-(3-[1,8]Naphthyridin-2-yl-propyl)-indol-1-yl]-acrylic acid methyl ester

The title compound was synthesized from 2-[3-(1H-indol-5-yl)-propyl]-[1,8]naphthyridine using the procedure described in Example 17, step (a), in 78% yield as an E/Z isomeric mixture. Mass Spectrum (LCMS, ESI) calculated for C₂₃H₂₂N₃O₂372.2 (M+H); found 372.3.

c) 3-[5-(3-[1,8]Naphthyridin-2-yl-propyl)-indol-1-yl]-propionic acid ethyl ester

To a solution of 2-[3-(1H-indol-5-yl)-propyl]-[1,8]naphthyridine (0.180 g, 0.627 mmol) in DMF (2 mL) was added sodium hydride (24.0 mg. 1.00 mmol) at 0° C. The reaction mixture was warmed to room temperature and stirred for 2 h. After cooling to 0° C., 3-chloro-propionic acid ethyl ester (85.0 mg, 0.63 mmol) was added and stirred overnight at room temperature. Ice water was added and the resulting mixture was extracted with methylene chloride. The combined organic layers were washed with water and brine, and dried over Na₂SO₄. Chromatography of the crude product on silica gel (methylene chloride/methanol, 95:5) gave the title product (0.11 g, 45% yield). ¹H NMR (CDCl₃) δ 9.08 (d, 1H), 8.13 (d, 1H), 8.08 (d, 1H), 7.30-7.50 (m, 4H), 7.10 (m, 2H), 6.40 (dd, 1H), 4.40 (t, 2H), 4.10 (m, 2H), 3.10 (t, 2H), 2.80 (m, 4H), 2.25 (m, 2H), 1.20 (q, 3H).

d) 3-{5-[3-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-propyl]-indol-1-yl}-propionic acid ethyl (and methyl)esters

3-[5-(3-[1,8]Naphthyridin-2-yl-propyl)-indol-1-yl]-propionic acid ethyl ester (0.11 g, 0.29 mmol) in methanol (5 mL) was stirred under hydrogen in the presence of 10% palladium on carbon (30.0 mg) for 24 h. After removal of solvent, the crude product was used in next reaction without further purification.
For ethyl ester: ¹H NMR (CDCl₃) δ 7.42 (s, 1H), 7.24 (d, J=8.2 Hz, 2H), 7.05 (m, 2H), 6.39 (d, J=2.9, 1H), 6.34 (d, J=7.4 Hz, 1H), 4.90 (br, 1H), 4.41 (t, J=6.8 Hz, 2H), 4.11 (m, 4H), 3.37 (m, 2H), 2.58-2.80 (m, 6H), 2.03 (m, 2H), 1.88 (m, 2H), 1.29 (m, 3H).
For methyl ester: Mass Spectrum (LCMS, ESI) calculated for C₂₃H₂₈N₃O₂378.3 (M+H); found 378.3.

e) 3-{5-[3-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-propyl]-indol-1-yl}-propionic acid

A mixture of 3-{5-[3-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-propyl]-indol-1-yl}-propionic acid ethyl (or methyl) esters (0.10 g, 0.26 mmol) and sodium hydroxide (0.06 g, 1.58 mmol) in tetrahydrofuran/water (7.5 mL, 3:1) was stirred at room temperature for 3 days. After neutralizing with 1.0 N HCl, the crude product was extracted with ethyl acetate and purified via column chromatography (methylene chloride/methanol) (95:5) to give the title compound as a white solid (49% yield). ¹H NMR (CDCl₃) δ 13.97 (br, 1H), 8.98 (br, 1H), 7.38 (d, J=7.3 Hz, 1H), 7.34 (d, J=1.1 Hz, 1H), 7.18 (m, 1H), 7.02 (dd, J=1.6 and 8.4 Hz, 1H), 6.44 (d, J=7.4 Hz, 1H), 6.36 (dd, J=0.6, 4.0 Hz, 1H), 4.39 (t, J=6.7 Hz, 2H), 3.38 (m, 2H), 2.79 (m, 6H), 1.99 (m, 4H), 1.83 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₂H₂₆N₃O₂364.2 (M+H); found 364.3.

EXAMPLE 50

3-{5-[3-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-propyl]-indol-1-yl}-hexanoic acid

a) 3-[5-(3-[1,8]Naphthyridin-2-yl-propyl)-indol-1-yl]-hexanoic acid ethyl ester

To a solution of 2-[3-(1H-indol-5-yl)-propyl]-[1,8]naphthyridine (0.18 g, 0.62 mmol) in DMF (2 mL) was added sodium hydride (30.0 mg, 1.24 mmol) at room temperature. After stirring for 15 minutes, 3-bromo-hexanoic acid ethyl ester (276 mg, 1.24 mmol) was added. The reaction mixture was stirred overnight and quenched with water. The crude product was extracted with methylene chloride, washed with brine, and purified via column chromatography with silica gel (ethyl acetate/hexane1:1), to give the title product (17% yield). ¹H NMR (CDCl₃) δ 9.08 (dd, J=1.7, 4.0 Hz, 1H), 8.14 (m, 1H), 8.06 (m, 1H), 7.32-7.44 (m, 4H), 7.05-7.12 (m, 2H), 6.45 (d, J=3.2 Hz, 1H), 4.81 (m, 1H), 3.99 (m, 2H), 3.08 (m, 2H), 2.83 (m, 4H), 2.22 (m, 2H), 1.90 (m, 2H), 1.20 (m, 2H), 1.07 (m, 3H), 0.87 (m, 3H). Mass Spectrum (LCMS, ESI) calculated for C₂₇H₃₂N₃O₂430.3 (M+H); found 430.3.

b) 3-{5-[3-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-propyl]-indol-1-yl}-hexanoic acid ethyl ester

A mixture of 3-[5-(3-[1,8]naphthyridin-2-yl-propyl)-indol-1-yl]-hexanoic acid ethyl ester (100 mg, 0.665 mmol) and 10% palladium on carbon (30 mg) in methanol (5 mL) was stirred under hydrogen for 2 days. The reaction solution was filtered through celite and dried to give the crude product, which was purified via column chromatography eluting with hexane/ethyl acetate (4/1), to give the title compound (80% yield). ¹H NMR (CDCl₃) δ 7.39 (d, J=0.9 Hz, 1H), 7.31 (d, J=8.5 Hz, 1H), 7.02-7.10 (m, 3H), 6.44 (dd, J=3.6, 6.3 Hz, 1H), 6.33 (d, J=7.3 Hz, 1H), 5.60 (br, 1H), 4.82 (m, 1H), 3.97 (q, 2H), 3.35 (t, 2H), 2.60-2.80 (m, 8H), 1.80-2.10 (m, 6H), 1.20 (m, 2H), 1.11 (m, 3H), 0.87 (m, 3H). Mass Spectrum (LCMS, ESI) calculated for C₂₇H₃₆N₃O₂434.3 (M+H); found 434.4.

c) 3-{5-[3-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-propyl]-indol-1-yl}-hexanoic acid

A mixture of 3-{5-[3-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-propyl]-indol-1-yl}-hexanoic acid ethyl ester (83.0 mg, 0.218 mmol) and NaOH (52.0 mg, 1.31 mmol) in THF/H₂O (3:1) was stirred at room temperature for 2 days. Aqueous HCl solution (1 N) was added to adjust the pH to 4-5. The crude product was extracted with ethyl acetate, and the combined organic layers were washed with brine and dried over Na₂SO₄. Removal of solvent gave the crude product, which was purified via column chromatography, eluting with 5% methanol in methylene chloride, to give the title compound (65% yield). ¹H NMR (CDCl₃) δ 9.35 (br, 1H), 7.42 (d, J=8.1 Hz, 1H) 7.27 (m, H), 7.16-7.20 (m, 2H), 6.95 (d, J=7.9 Hz, 1H), 6.40 (d, J=2.5 Hz, 1H), 6.22 (d, J=7.2 Hz, 1H), 4.91 (br, 1H), 3.40 (m, 2H), 2.56-2.82 (m, 10H), 1.84-2.05 (m, 6H), 0.86 (m, 3H). Mass Spectrum (LCMS, ESI) calculated for C₂₅H₃₂N₃O₂406.3 (M+H); found 406.4.

EXAMPLE 51

3-Phenyl-3-{5-[3-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-propyl]-indol-1-yl}-propionic acid

a) 3-[5-(3-[1,8]Naphthyridin-2-yl-propyl)-indol-1-yl]-3-phenyl-acrylic acid ethyl ester

A mixture of 2-[3-(1-triisopropylsilanyl-1H-indol-5-yl)-propyl]-[1,8]naphthyridine (125 mg, 0.282 mmol), phenyl-propynoic acid ethyl ester (98.0 mg, 0.563 mmol), and tetrabutylammonium fluoride [1.0 M] 0.85 mL, 0.85 mmol) in THF (3 mL) was stirred for 24 h. After removal of the solvent, the crude reaction mixture was purified via column chromatography on silica gel with ethyl acetate/hexane (2:1) to give the title product as an E/Z isomeric mixture in 64% yield. Mass Spectrum (LCMS, ESI) calculated for C₃₀H₂₈N₃O₂462.2 (M+H); found 462.3.

b). 3-Phenyl-3-{5-[3-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-propyl]-indol-1-yl}-propionic acid ethyl ester

3-[5-(3-[1,8]Naphthyridin-2-yl-propyl)-indol-1-yl]-3-phenyl-acrylic acid ethyl ester (30.0 mg, 0.484 mmol) in methanol (2 mL) was stirred under hydrogen in the presence of 10% palladium on carbon (15.0 mg) at room temperature for 3 days. Then, the reaction mixture was filtered through celite and purified via column chromatography on silica gel (methylene chloride/methanol) (95/5) to give the title product as yellow oil (20.0 mg, 66% yield). ¹H NMR (CDCl₃) δ 8.2 (d, 1H), 8.0 (m, 1H), 7.45 (m, 2H), 7.27 (m, 2H), 6.9-7.2 (m, 4H), 6.5 (1H), 6.3 (1H), 6.20 (m, 1H), 6.10 (m, 1H), 4.15 (m, 2H), 3.4 (m, 2H), 3.3 (m, 4H), 2.6 (m, 4H), 2.1 (m, 2H), 1.89 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₃₀H₃₄N₃O₂468.27 (M+H); found 468.3.

c) 3-Phenyl-3-{5-[3-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-propyl]-indol-1-yl}-propionic acid

A mixture of 3-phenyl-3-{5-[3-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-propyl]-indol-1-yl}-propionic acid ethyl ester (0.020 g, 0.04 mmol) and sodium hydroxide (0.01 g, 0.25 mmol) in THF/H₂O [1/0.3] (1.3 mL) was stirred at 50° C. for 24 h. The reaction mixture was neutralized with 1.0 N HCl to pH 5 and extracted with ethyl acetate. After removal of solvent, the crude product was purified via column chromatography, eluting with methylene chloride/methanol (95/5) to give the title compound (15% yield) as white solid. ¹H NMR (CDCl₃) δ 10.76 (br, 1H), 8.16 (br, 1H), 7.57 (br, 1H), 7.10-7.45 (m, 8H), 7.03 (m, 1H), 6.95 (m, 2H), 6.18 (m, 1H), 3.38 (m, 2H), 3.21 (m, 2H), 2.61 (m, 2H), 2.43 (m, 2H), 2.03 (m, 2H), 1.82 (m 2H), 1.70 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₈H₃₀N₃O₂440.2 (M+H); found 440.3.

d) 3-Phenyl-3-{5-[3-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-propyl]-indol-1-yl}-acrylic acid

The title compound was synthesized from 3-[5-(3-[1,8]naphthyridin-2-yl-propyl)-indol-1-yl]-3-phenyl-acrylic acid ethyl ester using the procedures described in Example 50, step (b) (isolated as a minor product) and step (c), in 10% yield as an E/Z isomeric mixture. ¹H NMR (CDCl₃) δ 7.10-7.35 (m, 7H), 7.00 (m, 1H), 6.92 (d, J=3.3 Hz, 1H), 6.46 (d, J=3.3 Hz, 1H), 6.31 (s, 1H), 6.22 (d, J=7.3 Hz, 1H), 6.13 (m, 1H), 3.37 (m, 2H), 2.64 (m, 2H), 2.56 (m, 2H), 2.47 (m, 2H), 2.04 (m, 2H), 1.84 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₈H₂₈N₃O₂437.3 (M+H); found: 438.4.

EXAMPLE 52

3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-[5-(2,2,2-trifluoro-ethoxy)-pyridin-3-yl]-propionic acid

a) 3-Bromo-5-(2,2,2-trifluoro-ethoxy)-pyridine

To a slurry of sodium hydride (60% dispersion in mineral oil, 0.54 g, 14 mmol) in DMF (15 mL) was added commercially available 2,2,2-trifluoroethanol (0.97 mL, 14 mmol) at room temperature. After stirring for 15 minutes, a solution of 3,5-dibromopyridine (3.2 g, 14 mmol) in 5 mL of DMF was added dropwise. The reaction mixture was heated overnight at 70° C. After cooling to room temperature, the reaction was diluted with water and extracted with ethyl acetate. The extracts were dried over magnesium sulfate and the solvent was removed under reduced pressure. The crude product was chromatographed on silica (5% ethyl acetate/hexanes) to give the title compound (1.6 g, 46% yield) as clear oil. ¹H NMR (CDCl₃) δ 8.42 (d, 1H, J=1.7 Hz), 8.32 (d, 1H, J=2.5 Hz), 7.46 (m, 1H), 4.42 (m, 2H).

b) 3-Triethoxyprop-1-ynyl-5-(2,2,2-trifluor-ethoxy)-pyridine

The title compound was synthesized from 3-bromo-5-(2,2,2-trifluoro-ethoxy)-pyridine and 3,3,3-triethoxypropyne using the procedure described in Example 43, step (e), in 46% yield. ¹H NMR (CDCl₃) δ 8.44 (bs, 1H), 8.35 (bs, 1H), 7.34 (m, 1H), 4.40 (q, 2H, J=7.9 Hz), 3.76 (q, 6H, J=7.1 Hz), 1.28 (t, 9H, J=7.1 Hz).

c) [5-(2,2,2-Trifluoro-ethoxy)-pyridin-3-yl]-propynoic acid ethyl ester

The title compound was synthesized from 3-triethoxyprop-1-ynyl-5-(2,2,2-trifluor-ethoxy)-pyridine using the procedure described in Example 43, step (f), in 100% yield. ¹H NMR (CDCl₃) δ 8.55 (bs, 1H), 8.48 (bs, 1H), 7.43 (m, 1H), 4.43 (q, 2H, J=7.9 Hz), 4.33 (q, 2H, J=7.2 Hz), 1.38 (t, 3H, J=7.2 Hz).

d) 7-[2-(1-{2-Ethoxycarbonyl-1-[5-(2,2,2-trifluoro-ethoxy)-pyridin-3-yl]-vinyl}-1H-indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyrdine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-[2-(1H-indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester and [5-(2,2,2-trifluoro-ethoxy)-pyridin-3-yl]-propynoic acid ethyl ester using the procedure described in Example 16, step (d1), in 79% yield as an E/Z isomeric mixture. ¹H NMR (CDCl₃) δ 8.47 (d, 0.33H, J=2.6 Hz), 8.42 (d, 0.67H, J=2.6 Hz), 8.36 (bs, 1H), 7.31 (d, 1H, J=7.8 Hz), 7.23 (m, 0.33H), 7.16 (d, 0.33H, J=9.1 Hz), 7.12 (m, 0.67H), 7.09 (d, 0.33H, J=2.6 Hz), 7.02 (d, 0.67H, J=3.3 Hz), 6.99 (m, 0.67H), 6.93 (d, 1H, J=7.8 Hz), 6.83, (m, 1H), 6.74 (m, 0.67H), 6.59 (d, 0.67H, J=3.3 Hz), 6.52 (dd, 0.67H, J=0.48, 2.8 Hz), 6.26 (s, 0.33H), 6.25 (s, 0.67H), 4.35 (m, 4H), 4.11 (m, 2H), 3.75 (m, 2H), 3.20 (m, 2H), 2.73 (t, 2H, J=6.5 Hz), 1.92 (m, 2H), 1.52 (s, 9H), 1.19 (t, 1H, J=7.0 Hz), 1.03 (t, 2H, J=7.2 Hz).

e) 7-[2-(1-{2-Ethoxycarbonyl-1-[5-(2,2,2-trifluoro-ethoxy)-pyridin-3-yl]-ethyl}-1H-indol-5-yloxy)-ethyl-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-[2-(1-{2-ethoxycarbonyl-1-[5-(2,2,2-trifluoro-ethoxy)-pyridin-3-yl]-vinyl}-1H-indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyrdine-1-carboxylic acid tert-butyl ester using the procedure described in Example 18, step (e), in 59% yield. ¹H NMR (CDCl₃) δ 8.27 (bs, 1H), 8.23 (bs, 1H), 7.30 (d, 1H, J=7.6 Hz), 7.16 (m, 2H), 7.09 (d, 1H, J=2.3 Hz), 6.92 (m, 2H), 6.82 (dd, 1H, J=2.3, 6.5 Hz), 6.47 (d, 1H, J=3.3 Hz), 6.02 (t, 1H, J=7.4 Hz), 4.35 (t, 2H, J=7.0 Hz), 4.09 (m, 4H), 3.74 (m, 2H), 3.18 (t, 2H, J=7.0 Hz), 2.71 (t, 2H, J=6.5 Hz), 1.91 (m, 2H), 1.50 (s, 9H), 1.11 (t, 3H, J=7.0 Hz).

f) 3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-[5-(2,2,2-trifluoro-ethoxy)-pyridin-3-yl]-propionic acid ethyl ester

The title compound was synthesized from 7-[2-(1-{2-ethoxycarbonyl-1-[5-(2,2,2-trifluoro-ethoxy)-pyridin-3-yl]-ethyl}-1H-indol-5-yloxy)-ethyl-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (f), in 71% yield. ¹H NMR (CDCl₃) δ 8.26 (bs, 1H), 8.24 (bs, 1H), 7.13 (m, 4H), 6.92 (m, 1H), 6.81 (dd, 1H, J=2.3, 6.5 Hz), 6.48 (m, 2H), 6.03 (t, 1H, J=7.4 Hz), 5.35 (bs, 1H), 4.27 (m, 4H), 4.06 (m, 2H), 3.34 (m, 2H), 3.28 (t, 2H, J=9.0 Hz), 3.05 (t, 2H, J=6.7 Hz), 2.68 (t, 2H, J=6.2 Hz), 1.88 (m, 2H), 1.12 (t, 3H, J=7.2 Hz).

g) 3-{5-[2-(5,6,7,8-Tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-[5-(2,2,2-trifluoro-ethoxy)-pyridin-3-yl]-propionic acid

The title compound was synthesized from 3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-3-[5-(2,2,2-trifluoro-ethoxy)-pyridin-3-yl]-propionic acid ethyl ester using the procedure described in Example 18, step (g), in 56% yield. ¹H NMR (DMSO-d₆) δ 8.27 (s, 1H), 8.25 (s, 1H), 7.70 (d, 1H, J=3.3 Hz), 7.58 (m, 1H), 7.48 (d, 1H, J=9.0 Hz), 7.03 (d, 1H, J=7.2 Hz), 7.00 (d, 1H, J=2.6 Hz), 6.69 (dd, 1H, J=2.3, 6.5 Hz), 6.37 (d, 1H, J=3.0 Hz), 6.34 (d, 1H, J=7.2 Hz), 6.31 (bs, 1H), 5.98 (m, 1H), 4.82 (q, 2H, J=8.8 Hz), 4.17 (t, 2H, J=6.7 Hz), 3.55 (m, 2H), 3.21 (m, 2H), 2.85 (t, 2H, J=6.7 Hz), 2.58 (t, 2H, J=6.2 Hz), 1.73 (m, 2H). ¹⁹F NMR (DMSO-d₆) δ −73.05 (t, 3F, J=8.8 Hz). Mass Spectrum (LCMS, ESI) calculated for C₂₈H₂₈F₃N₄O₄: 541.2 (M+1); found: 541.3.

EXAMPLE 53

3-(5-ethoxy-pyridin-3-yl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

a) 3-Bromo-5-ethoxy-pyridine

The title compound was synthesized from 3,5-dibromopyridine and ethanol using the procedure described in Example 53, step (a) in 60% yield. ¹H NMR (CDCl₃) δ 8.27 (bs, 1H), 8.23 (bs, 1H), 7.33 (m, 1H), 4.06 (q, 2H, J=7.0 Hz), 1.43 (t, 3H, J=7.0 Hz).

b) 3-Ethoxy-5-triethoxyprop-1-ynyl-pyridine

The title compound was synthesized from 3-bromo-5-ethoxy-pyridine and 3,3,3-triethoxypropyne using the procedure described in Example 43, step (e), in 37% yield. ¹H NMR (CDCl₃) δ 8.27 (bs, 1H), 8.24 (bs, 1H), 7.21 (m, 1H), 4.02 (q, 2H, J=7.0 Hz), 3.72 (q, 6H, J=7.0 Hz), 1.39 (t, 3H, J=7.0 Hz), 1.23 (t, 9H, J=7.0 Hz).

c) (5-Ethoxy-pyridin-3-yl)-propynoic acid ethyl ester

The title compound was synthesized from 3-ethoxy-5-triethoxyprop-1-ynyl-pyridine using the procedure described in Example 43, step (f), in 96% yield. ¹H NMR (CDCl₃) δ 8.40 (d, 1H, J=1.4 Hz), 8.35 (d, 1H, J=2.8 Hz), 7.33 (m, 1H), 4.32 (q, 2H, J=7.0 Hz), 4.08 (q, 2H, J=7.0 Hz), 1.46 (t, 3H, J=7.0), 1.38 (t, 3H, J=7.0 Hz).

d) 7-(2-{1-[2-Ethoxycarbonyl-1-(5-ethoxy-pyridin-3-yl)-vinyl-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from (5-ethoxy-pyridin-3-yl)-propynoic acid ethyl ester and 7-[2-(1H-indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (dl), in 70% yield. Mass Spectrum (LCMS, ESI) calculated for C₃₅H₄₀N₄O₆: 513.2 (M-Boc+H); found: 513.3 (-Boc).

e) 7-(2-{1-[2-Ethoxycarbonyl-1-(5-ethoxy-pyridin-3-yl)-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester

The title compound was synthesized from 7-(2-{1-[2-ethoxycarbonyl-1-(5-ethoxy-pyridin-3-yl)-vinyl-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 18, step (e), in 60% yield. ¹H NMR (CDCl₃) δ 8.19 (bs, 1H), 8.18 (bs, 1H), 7.31 (d, 1H, J=7.8 Hz), 7.19 (m, 2H), 7.10 (d, 1H, J=2.3 Hz), 6.95 (d, 1H, J=7.6 Hz), 6.84 (m, 2H), 6.47 (d, 1H, J=7.4 Hz), 6.03 (t, 1H, J=7.4 Hz), 4.38 (t, 2H, J=7.0 Hz), 4.07 (q, 2H, J=7.2 Hz), 3.95 (q, 2H, 7.0 Hz), 3.76 (m, 2H), 3.30 (t, 2H, J=8.3 Hz), 3.21 (t, 2H, J=7.0 Hz), 2.72 (t, 2H, J=8.3 Hz), 1.92 (m, 2H), 1.52 (s, 9H), 1.36 (t, 3H, J=7.0 Hz), 1.12 (t, 3H, J=7.2 Hz).

f) 3-(5-Ethoxy-pyridin-3-yl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy-indol-1-yl}-propionic acid ethyl ester

The title compound was synthesized from 7-(2-{1-[2-ethoxycarbonyl-1-(5-ethoxy-pyridin-3-yl)-ethyl]-1H-indol-5-yloxy}-ethyl)-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (f), in 71% yield. ¹H NMR (CDCl₃) δ 8.19 (bs, 1H), 8.16 (bs, 1H), 7.19 (m, 3H), 7.08 (d, 1H, J=2.3 Hz), 6.87 (t, 1H, J=2.0 Hz), 6.81 (dd, 1H, J=2.3, 6.5 Hz), 6.51 (d, 1H, J=7.4 Hz), 6.47 (1H, J=3.3 Hz), 6.05 (bs, 1H), 6.02 (t, 1H, J=7.7 Hz), 4.29 (t, 2H, J=6.5 Hz), 4.06 (q, 2H, J=7.2 Hz), 3.95 (q, 2H, J=7.0 Hz), 3.43 (m, 2H), 3.29 (t, 2H, J=8.3 Hz), 3.08(t, 2H, J=6.5 Hz), 2.71 (t, 2H, J=6.3 Hz), 1.91 (m, 2H), 1.36 (t, 3H, J=7.0 Hz), 1.13 (t, 3H, J=7.2 Hz).

g) 3-(5-Ethoxy-pyridin-3-yl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy-indol-1-yl}-propionic acid

The title compound was synthesized from 3-(5-ethoxy-pyridin-3-yl)-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy-indol-1-yl}-propionic acid ethyl ester using the procedure described in Example 18, step (g), in 69% yield. ¹H NMR (DMSO-d₆) δ 8.18 (bs, 1H), 8.13 (bs, 1H), 7.73 (d, 1H, J=3.0 Hz), 7.48 (d, 1H, J=9.0 Hz), 7.35 (s, 1H), 7.13 (m, 1H), 7.02 (d, 1H, J=2.3 Hz), 6.71 (dd, 1H, J=2.0, 7.0 Hz), 6.53 (bs, 1H), 6.41 (m, 2H), 5.99 (t, 1H, J=7.0 Hz), 4.20 (t, 2H, J=7.0 Hz), 4.05 (m, 2H), 3.49 (m, 4H), 2.90 (t, 2H, J=6.0 Hz), 2.62 (t, 2H, J=6.0 Hz), 1.75 (m, 2H), 1.29 (t, 3H, J=7.0 Hz). Mass Spectrum (LCMS, ESI) calculated for C₂₈H₃₁N₄O₄: 487.2 (M+1); found: 487.3.

EXAMPLE 54

3-Pyridin-4-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

a) Pyridin-4-yl-propynoic acid ethyl ester

The title compound is prepared from commercially available material 3-oxo-3-pyridin-4-yl-propionic acid ethyl ester using the procedure described in Example 32, step (b), in 74% yield. ¹H NMR (CDCl₃) δ 8.69 (m, 2H), 7.43 (m, 2H), 4.32 (q, 2H), 1.45 (t, 3H).

The title compound is prepared from pyridin-4-yl-propynoic acid ethyl ester and 7-[2-(1H-Indol-5-yloxy)-ethyl]-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 17, step (a), in 64% yield as an E/Z mixture. The mixture is used for the next reaction without further separation.

The title compound is synthesized from 7-{2-[1-(2-Ethoxycarbonyl-1-pyridin-4-yl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 18, step (e), in 43% yield. ¹H NMR (CDCl₃) δ 8.53 (d, 2H), 7.35 (m, 1H), 7.28 (m, 1H), 7.13 (m, 2H), 7.02 (d, 2H), 6.96 (m, 1H), 6.84 (m, 1H), 6.50 (m, 1H), 6.00 (t, 1H), 4.40 (t, 2H), 4.10 (q, 2H), 3.77 (t, 2H), 3.32(m, 2H), 3.25 (m, 2H), 2.75 (m, 2H), 1.92 (m, 2H), 1.52 (s, 9H), 1.15 (t, 3H). Mass Spectrum (LCMS, ESI) calculated for C₃₃H₃₉N₄O₅571.29 (M+H); found 471.4 (M-Boc+H, 100%).

d) 3-Pyridin-4-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester

The title compound is synthesized from 7-{2-[1-(2-Ethoxycarbonyl-1-pyridin-4-yl-ethyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (e), in 36% yield. ¹H NMR (CDCl₃) δ 8.52 (d, 2H), 7.16 (d, 1H), 7.10 (m, 2H), 7.00 (d, 1H), 6.83 (m, 1H), 6.48 (m, 2H), 6.00 (m, 1H), 4.30 (m, 2H), 4.12 (m, 2H), 3.40 (m, 2H), 3.28 (m, 2H), 3.05 (m, 2H), 2.70 (m, 2H), 1.90 (m, 2H), 1.20 (t, 3H). Mass Spectrum (LCMS, ESI) calculated for C₂₈H₃₁N₄O₃471.24 (M+H); found 471.3.

e) 3-Pyridin-4-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid

The title compound is synthesized from 3-pyridin-4-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-propionic acid ethyl ester using the procedure described in Example 16, step (f), in 66% yield. ¹H NMR (CDCl₃) δ 8.44 (br, 2H), 7.38 (m, 1H), 7.20 (d, 1H), 7.18 (d, 1H), 7.15 (m, 1H), 7.02 (m, 1H), 6.65 (dd, 1H), 6.49 (m, 1H), 6.35 (m, 1H), 6.10 (m, 1H), 3.70 (m, 4H), 3.38 (m, 2H), 3.20 (m, 2H), 2.67 (m, 2H), 1.85 (m, 2H). Mass Spectrum (LCMS, ESI) calculated for C₂₆H₂₇N₄O₃443.21 (M+H); found 443.2.

EXAMPLE 55

3-Pyridin-2-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-acrylic acid

a) 3-Pyridin-2-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-acrylic acid ethyl ester

The title compound is prepared from 7-{2-[1-(2-Ethoxycarbonyl-1-pyridin-2-yl-vinyl)-1H-indol-5-yloxy]-ethyl}-3,4-dihydro-2H-[1,8]naphthyridine-1-carboxylic acid tert-butyl ester using the procedure described in Example 16, step (e), in 74% yield. ¹H NMR (CDCl₃) δ 8.50 (d, 1H), 8.0 (s, 1H), 7.77 (t, 1H), 7.45 (m, 1H), 7.10 (d, 2H), 7.00 (d, 1H), 6.90 (d, 1H), 6.78 (dd, 1H), 6.60 (m, 1H), 6.50 (d, 1H), 6.30 (s, 1H), 4.90 (br, 1H), 4.30 (t, d, 2H), 4.13 (m, 2H), 3.40 (m, 2H), 3.10 (t, 2H), 2.70 (t, 2H), 1.90 (m, 2H), 1.15 (t, 3H).

b) 3-Pyridin-2-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-acrylic acid

The title compound is prepared from 3-pyridin-2-yl-3-{5-[2-(5,6,7,8-tetrahydro-[1,8]naphthyridin-2-yl)-ethoxy]-indol-1-yl}-acrylic acid ethyl ester using the procedure described in Example 16, step (f), in 54% yield. ¹H NMR (CDCl₃) δ 8.9 (br, 1H), 8.6 (m, 1H), 7.60 (m, 1H), 7.25 (m, 2H), 7.20 (d, 1H), 7.08 (m, 2H), 6.98 (m, 1H), 6.72 (m, 1H), 6.50 (m, 2H), 6.42 (d, 1H), 4.20 (m, 2H), 3.40 (m, 2H), 2.95 (m, 2H), 2.70 (m, 2H), 1.90 (2H). Mass Spectrum (LCMS, ESI) calculated for C₂₆H₂₄N₄O₃440.27 (M+H); found 441.3.

EXAMPLE 56

Preparation of 6-(2-hydroxy-ethyl)-2,3-dihydro-pyrido[3,2-b][1,4]oxazine-4-carboxylic acid tert-butyl ester

a) 2-Amino-6-methyl-pyridin-3-ol

A mixture of 6-methyl-2-nitro-pyridin-3-ol (18.5 g, 0.120 mmol), 10% palladium on activated carbon (1.35 g), and ethyl acetate (240 mL) was hydrogenated for 3 days. The mixture was filtered through Celite and washed with methanol/ethylacetate (5%). The filtrate and washing were combined and concentrated to give the title compound (14.7 g, 99% yield) as a pale brown solid. ¹H NMR (DMSO-d₆) δ 9.19 (bs, 1H), 6.73 (d, 1H, J=7.6 Hz), 6.12 (d, 1H, J=7.6 Hz), 5.36 (bs, 2H), 2.15 (s, 3H).

b) 6-Methyl-4H-pyrido[3,2-b][1,4]oxazin-3-one L. Savelon, et. al, Bioorganic & Medicinal Chemistry, 6, 133, (1998)

To a suspension of 2-amino-6-methyl-pyridin-3-ol (18.3 g, 148 mmol), sodium bicarbonate (30 g, 354 mmol), H₂O (100 mL), and 2-butanone (100 mL) in an ice-water bath was added a solution of chloroacetyl chloride (13.3 mL. 167 mmol) in 2-butanone (30 mL) over 1.5 h, controlling the temperature below 10° C. After the addition was complete, the ice-water bath was removed and the mixture was stirred at ambient temperature for 30 minutes, followed by refluxing for 1.5 h. The solvents were evaporated, and the resulting solid washed with H₂O (3 times), and dried under high vacuum overnight, giving the title compound (19.2 g, 79% yield) as a pale yellow solid. ¹H NMR (CDCl₃) δ 10.45 (bs, 1H), 7.17 (d, 1H, J=8.1 Hz), 6.78 (d, 1H, J=8.1 Hz), 4.62 (s, 2H), 2.52 (s, 3H). Mass spectrum (LCMS, ESI) calculated for C₈H₉N₂O₂165.1 (M+1); found 165.1.

c) 6-Methyl-3,4-dihydro-2H-pyrido[3,2-b][1,4]oxazine

A flask was charged with lithium aluminum hydride (607 mg, 16.0 mmol) was placed in an ice-water bath under an argon atmosphere. THF (13 mL) was added slowly. To this suspension was added slowly a solution of 6-methyl-4H-pyrido[3,2-b][1,4]oxazin-3-one (1.05 g, 6.40 mmol) in THF (13 mL). After the addition was completed, additional THF (9 mL) was added, and the reaction was stirred in the ice-water bath for 30 minutes. Ice-water bath was removed, the mixture was stirred at ambient temperature for 3 h. The mixture was cooled with an ice-water bath, and H₂O (0.86 mL) was added slowly, followed by cooled aqueous NaOH solution (0.64 mL, 10%). The ice-water bath was removed, additional H₂O (1.8 mL) was added. After stirring for 30 minutes, Celite and Na₂SO₄were added. The mixture was filtered through Celite, and the Celite washed with EtOAc. The filtrate and the washing were combined, dried over Na₂SO₄, and concentrated to give the title compound (0.96 g, quantitative yield) as a while solid. ¹H NMR(CDCl₃) δ 6.85 (d, 1H, J=8.0 Hz), 6.35 (d, 1H, J=8.0 Hz), 6.08 (bs, 1H), 4.19-4.16 (m, 2H), 3.54-3.52 (m, 2H), 2.31 (s, 3H).

d) 6-Methyl-2,3-dihydro-pyrido[3,2-b][1,4]oxazine-4-carboxylic acid tert-butyl ester

A mixture of 6-methyl-3,4-dihydro-2h-pyrido[3,2-b][1,4]oxazine (0.89 g, 5.93 mmol) and di-tert-butyl dicarbonate was heated and stirred at 60° c. for 48 h, and then allowed to cooled to ambient temperature to give crude product. Recrystallization of the crude product from hexane gave the title compound (118 g, 80% yield) as a white solid. This crude product was used in next step reaction without further purification.

e) 6-tert-Butoxycarbonylmethyl-2,3-dihydro-pyrido[3,2-b][1,4]oxazine-4-carboxylic acid tert-butylester

To a solution of diisopropylamine (1.23 ml, 8.80 mmol) in thf (8.0 ml) at −78° c. was added n-butyllithium (3.26 ml, 2.5 m in hexanes) and stirred for 20 min. To the above solution was added a solution of 6-tert-butoxycarbonylmethyl-2,3-dihydro-pyrido[3,2-b][1,4]oxazine-4-carboxylic acid tert-butylester (1.1 g, 4.40 mmol) in thf (15 ml) over a period of 30 min. After the addition completed, the mixture was stirred for 40 min. Diethylcarbonate (0.85 ml, 7.04 mmol) was added at once and stirred for 15 min. Dry ice-acetone bath was removed. The mixture was stirred in an ice water bath for 1 h. Saturated NH₄Cl was added. The mixture was diluted with ethyl acetate. The organic layer was separated, washed with h₂O, brine, dried over na₂so₄, concentrated, and flash chromatographed on silica gel, eluting with ethyl acetate/hexane (5, 10, 15, 25, 30%) to give the title compound (755 mg, 49% yield) as a yellow oil. ¹H NMR(CDCl₃) δ 7.13 (d, 1H, j=8.2 Hz), 6.97 (d, 1H, j=8.2 Hz), 4.23 (t, 2H, j=4.4 Hz), 3.89 (t, 2H, j=4.5 Hz), 3.65 (s, 2H), 1.54 (s, 9H), 1.45 (s, 9H).

f) 2-(3,4-dihydro-2h-pyrido[3,2-b][1,4]oxazin-6-yl)-ethanol

To a solution of 6-tert-butoxycarbonylmethyl-2,3-dihydro-pyrido[3,2-b][1,4]oxazine-4-carboxylic acid tert-butyl ester (350 mg, 1 mmol) in THF (4.0 ml) was added a solution of lithium borohydride (0.6 ml, 2.0 m in thf). The mixture was refluxed overnight, then cooled in an ice-bath. Aqueous solution of naoh (0.36 ml, 5%) was added. The ice-bath was removed. Additional h₂O (0.36 ml) was added and the mixture stirred for 10 min. Celite and na₂so₄were added. The mixture was filtered through celite, and the celite washed with etoac. The filtrate and washing were combined, dried over na₂so₄, concentrated, and flash chromatographed on silica gel, eluting with meoh/dcm (1, 2, 3, 4%) to give the product (171 mg, 94% yield) as a yellow oil. ¹H NMR(CDCl₃) δ6.90 (d, 1H, J=7.8 Hz), 6.39 (d, 1H, J=7.7 Hz), 4.85 (bs, 1H), 4.20 (t, 2H, J=4.4 Hz), 3.91 (t, 2H, J=5.5 Hz), 3.69-3.52 (m, 2H), 2.78 (t, 2H, J=5.6 Hz).

EXAMPLE 57

In Vitro Inhibition of Purified Enzymes

Fibrinogen-IIb-IIIa Assay
The assay is based on the method of Dennis (Dennis, M. S., et al., Proteins 15: 312-321 (1993)). Costar 9018 flat-bottom 96-well ELISA plates were coated overnight at 4□C with 100 μL/well of 10 μg/mL human fibrinogen (Calbiochem 341578) in 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM CaCl₂, 0.02% NaN₃(TAC buffer), and blocked for 1 hr at 37□C with 150 μL/well of TAC buffer containing 0.05% Tween 20 and 1% bovine serum albumin (TACTB buffer). After washing 3 times with 200 μL/well of 10 mM Na₂HPO₄pH 7.5, 150 mM NaCl, 0.01% Tween 20 (PBST buffer), controls or test compound (0.027-20.0 μM) were mixed with 40 μg/mL human GPIIbIIIa (Enzyme Research Laboratories) in TACTB buffer, and 100 μL/well of these solutions were incubated for 1 hr at 37° C. The plate was then washed 5 times with PBST buffer, and 100 μL/well of a monoclonal anti-GPIIbIIIa antibody in TACTB buffer (1 μg/mL, Enzyme Research Laboratories MabGP2b3a) was incubated at 37□C for 1 hr. After washing (5 times with PBST buffer), 100 μL/well of goat anti-mouse IgG conjugated to horseradish peroxidase (Kirkegaard & Perry 14-23-06) was incubated at 37° C. for 1 hr (25 ng/mL in PBST buffer), followed by a 6-fold PBST buffer wash. The plate was developed by adding 100 μL/well of 0.67 mg o-phenylenediamine dihydrochloride per mL of 0.012% H₂O₂, 22 mM sodium citrate, 50 mM sodium phosphate, pH 5.0 at room temperature. The reaction was stopped with 50 μL/well of 2M H₂SO₄, and the absorbence at 492 nm was recorded. Percent (%) inhibition was calculated from the average of three separate determinations relative to buffer controls (no test compound added), and a four parameter fit (Marquardt, D. W., J. Soc. Indust. Appl Math. 11:431-441 (1963)) was used to estimate the half maximal inhibition concentration (IC₅₀).
α_vβ₃-Vitronectin Assay
The assay was based on the method of Niiya (Niiya, K., et al., Blood 70:475-483 (1987)). Costar 9018 flat-bottom 96-well ELISA plates were coated overnight at room temperature with 100 μL/well of 0.4 μg/mL human α_vβ₃(Chemicon CC1019) in TS buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM CaCl₂, 1 mM MgCl₂, 1 mM MnCl₂). All subsequent steps were performed at room temperature. Plates were blocked for 2 hr with 150 μL/well of TS buffer containing 1% BSA (TSB buffer), and washed 3 times with 200 μL/well of PBST buffer. Controls or test compound (0.0001-20.0 μM) were mixed with 1 μg/mL of human vitronectin (Chemicon CC080) that had been biotinylated in-house with sulfo-NHS-LC-LC-biotin (Pierce 21338, 20:1 molar ratio), and 100 μL/well of these solutions (in TSB buffer) were incubated for 2 hr. The plate was then washed 5 times with PBST buffer, and 100 μL/well of 0.25 μg/mL NeutrAvidin-horseradish peroxidase conjugate (Pierce 31001) in TSB buffer was incubated for 1 hr. Following a 5-fold PBST buffer wash, the plate was developed and results were calculated as described for the fibrinogen-IIbIIIa assay. IC₅₀values for inhibition of the α_vβ₃-vitronectin interaction by other compounds of the invention are presented in Table 1.

TABLE 1

In Vitro Activity of New A_vB₃Antagonists

Example # IC₅₀(nM)

1 500

4 670

5 50

7 500

14 4.00

15 6.00

38 0.24

α_vβ₅-Vitronectin Assay
The assay is similar to the α_vβ₃-vitronectin assay. Costar 9018 flat-bottom 96-well ELISA plates were coated overnight at room temperature with 100 μL/well of 1 μg/mL human α_vβ₅(Chemicon CC1025) in TS buffer. All subsequent steps were preformed at room temperature. Plates were blocked for 2 hr at 30□C with 150 μL/well of TSB buffer, and washed 3 times with 200 μL/well of PBST buffer. Controls or test compound (0.0001-20 μM) were mixed with 1 μg/mL of human vitronectin (Chemicon CC080) that had been biotinylated in-house with sulfo-NHS-LC-LC-biotin (Pierce 21338, 20:1 molar ratio), and 100 μL/well of these solutions (in TSB buffer) were incubated for 2 hr. The plate was then washed 5 times with PBST buffer, and 100 μL/well of 0.25 μg/mL. NeutraAvidin-horseradish peroxidase conjugate (Pierce 31001) in TSB buffer was incubated at 30° C. for 1 hr. Following a 5-fold PBST buffer wash, the plate was developed and results were calculated as described for the fibrinogen-IIbIIIa assay.

EXAMPLE 58

Tablet Preparation

Tablets containing 25.0, 50.0, and 100.0 mg, respectively, of the compound of Example 1 (“active compound”) are prepared as illustrated below

TABLET FOR DOSES CONTAINING FROM

25-100 MG OF THE ACTIVE COMPOUND

Amount-mg

Active compound 25.0 50.0 100.00

Microcrystalline cellulose 37.25 100.0 200.0

Modified food corn starch 37.25 4.25 8.5

Magnesium stearate 0.50 0.75 1.5
All of the active compound, cellulose, and a portion of the corn starch are mixed and granulated to 10% corn starch paste. The resulting granulation is sieved, dried and blended with the remainder of the corn starch and the magnesium stearate. The resulting granulation is then compressed into tablets containing 25.0, 50.0, and 100.0 mg, respectively, of active ingredient per tablet.

EXAMPLE 59

Intravenous Solution Preparation

An intravenous dosage form of the compound of Example 1 (“active compound”) is prepared as follows:

Active compound 0.5-10.0 mg

Sodium citrate 5-50 mg

Citric acid 1-15 mg

Sodium chloride 1-8 mg

Water for injection (USP) q.s. to 1 ml
Utilizing the above quantities, the active compound is dissolved at room temperature in a previously prepared solution of sodium chloride, citric acid, and sodium citrate in Water for Injection (USP, see page 1636 of United States Pharmacopeia/National Formulary for 1995, published by United States Pharmacopeial Convention, Inc., Rockville, Md. (1994).
Having now fully described this invention, it will be understood to those of ordinary skill in the art that the same can be performed within a wide and equivalent range of conditions, formulations, and other parameters without affecting the scope of the invention or any embodiment thereof. All patents and publications cited herein are fully incorporated by reference herein in their entirety.

Claims

1-65. (canceled)

66. A compound having the Formula IV:

wherein:

R¹, R², R³, R⁴and R⁵independently represent hydrogen, halogen, alkyl, aryl, aralkyl, heteroaryl or heteroarylalkyl;

R⁶, R⁷, R⁸and R⁹independently represent hydrogen, alkyl, hydroxyalkyl, aminoalkyl, monoalkylaminoalkyl, dialkylaminoalkyl, carboxyalkyl, aryl or aralkyl;

or R⁶and R⁷are taken together to form —(CH₂)_p—, where p is 2-8, while R⁸and R⁹are defined as above; or R⁸and R⁹are taken together to form —(CH₂)_q—, where q is 2-8, while R⁶and R⁷are defined as above; or R⁶and R⁸are taken together to form —(CH₂)_r—, while r is zero (a bond), 1 or 2, while R⁷and R⁹are defined as above;

X represents oxygen, sulfur, —CH₂—, —NH—, —(C═O)NH— or —NH(C═O)—;

n is from 0 to 4;

m is from 0 to 4;

a is 0 or 1;

D represents oxygen;

v is 0 or 1;

R¹⁰, R¹¹, R¹²and R¹³independently represent: hydrogen; hydroxy; alkyl; alkoxy; cycloalkyl; aryl, optionally substituted with one or more of halogen, hydroxy, cyano, alkyl, aryl, alkoxy, haloalkyl, arylalkyl, arylalkoxy, aryloxy, alkylsulfonyl, alkylsulfinyl, alkylalkoxyaryl, monoalkylamino, dialkylamino, aminoalkyl, monoalkylaminoalkyl, dialkylaminoalkyl, alkanoyl; monoalkylamino; dialkylamino; aminoalkyl; monoalkylaminoalkyl; dialkylaminoalkyl; alkanoyl; heteroaryl having 5-14 ring members, optionally substituted with one or more of halogen, hydroxy, cyano, alkyl, aryl, alkoxy, haloalkyl, arylalkyl, arylalkoxy, aryloxy, alkylsulfonyl, alkylsulfinyl, alkylalkoxyaryl, monoalkylamino, dialkylamino, aminoalkyl, monoalkylaminoalkyl, dialkylaminoalkyl, alkanoyl; or

wherein R¹⁷and R¹⁸together form —CH₂CH₂—O—, —O—CH₂CH₂—, —O—CH₂—O— or —O—CH₂CH₂—O—; or

R¹⁰and R¹²are taken together to form —(CH₂)_n—, wherein s is 0 (a bond) or 1 to 4, while R¹¹and R¹³are as defined as above; or R¹⁰and R¹²are taken together to form a double bond when i is 0 and k is 1, while R¹¹and R¹³are as defined above; or R¹⁰and R¹¹are taken together to form —(CH₂)_t—, wherein t is 2 to 8, while R¹²and R¹³are as defined as above, or R¹²and R¹³are taken together to form —(CH₂)_n— wherein u is 2 to 8, while R¹⁰and R¹¹are as defined as above;

i is from 0 to 4;

j is from 0 to 4;

k is 0 or 1;

R¹⁴is hydrogen or a functionality that acts as a prodrug (i.e., converts to the active species by an endogenous biological process such as an esterase, lipase, or other hydrolase), such as alkyl, aryl, aralkyl, dialkylaminoalkyl, 1-morpholinoalkyl, 1-piperidinylalkyl, pyridinylalkyl, alkoxy(alkoxy)alkoxyalkyl, or (alkoxycarbonyl)oxyethyl;

W is:

wherein:

R¹⁵is hydrogen, halogen, alkyl, aryl or arylalkyl; and

R¹⁶is hydrogen, alkyl, haloalkyl or halogen.

67. The compound of claim 66, wherein R¹⁴is a prodrug, selected from the group consisting of: alkyl, aryl, aralkyl, dialkylaminoalkyl, 1-morpholinoalkyl, 1-piperidinylalkyl, pyridinylalkyl, alkoxy(alkoxy)alkoxyalkyl, or (alkoxycarbonyl)oxyethyl.

68. The compound of claim 66, wherein:

R¹, R², R³, R⁴and R⁵independently represent hydrogen, halogen, (C_1-8)alkyl, (C_6-10)aryl, (C_6-10)ar(C_1-8)alkyl, 5-14 member heteroaryl, or 5-14 member heteroaryl(C_1-8)alkyl;

R⁶, R⁷, R⁸and R⁹independently represent hydrogen, (C_1-8)alkyl, hydroxy(C_1-8)alkyl, amino(C_1-8)alkyl, mono(C_1-8)alkylamino(C_1-8)alkyl, di(C_1-8)alkylamino(C_1-8)alkyl, carboxy(C_1-8)alkyl, (C_6-10)aryl or (C_6-10)ar(C_1-8)alkyl;

or R⁶and R⁷are taken together to form —(CH₂)_p—, where p is 2-8, while R⁸and R⁹are defined as above; or R⁸and R⁹are taken together to form —(CH₂)_q—, where q is 2-8, while R⁶and R⁷are defined as above; or R⁶and R³are taken together to form —(CH₂)_r—, while r is zero (a bond), 1 or 2, while R⁷and R⁹are defined as above;

n is from 0 to 4;

m is from 0 to 4;

a is from 0 or 1;

D represents oxygen;

v is from 0 or 1;

R¹⁰, R¹¹, R¹²and R¹³independently represent: hydrogen; hydroxy; (C_1-8)alkyl; (C_1-8)alkoxy; (C_3-8)cycloalkyl; (C_6-10)aryl, optionally substituted with one or more of halogen, hydroxy, cyano, (C_1-8)alkyl, (C_6-10)aryl, (C_1-8)alkoxy, halo(C_1-8)alkyl, (C_6-10)aryl(C_1-8)alkyl, (C_6-10)aryl(C_1-8)alkoxy, (C_6-10)aryloxy, (C_1-8)alkylsulfonyl, (C_1-8)alkylsulfinyl, (C_1-8)alkoxy(C_6-10)aryl(C_1-8)alkyl, mono(C_1-8)alkylamino, di(C_1-8)alkylamino, amino(C_1-8)alkyl, mono(C_1-8)alkylamino(C_1-8)alkyl, di(C_1-8)alkylamino(C_1-8)alkyl, (C_1-8)alkanoyl; mono(C_1-8)alkylamino; di(C_1-8)alkylamino; amino(C_1-8)alkyl; mono(C_1-8)alkylamino(C_1-8)alkyl; di(C_1-8)alkylamino(C_1-8)alkyl; (C_1-8)alkanoyl; heteroaryl having 5-14 ring members, optionally substituted with one or more of halogen, hydroxy, cyano, (C_1-8)alkyl, (C_6-10)aryl, (C_1-8)alkoxy, halo(C_1-8)alkyl, (C_6-10)aryl(C_1-8)alkyl, (C_6-10)aryl(C_1-8)alkoxy, (C_6-10)aryloxy, (C_1-8)alkylsulfonyl, (C_1-8)alkylsulfinyl, (C_1-8)alkoxy(C_6-10)aryl(C_1-8)alkyl, mono(C_1-8)alkylamino, di(C_1-8)alkylamino, amino(C_1-8)alkyl, mono(C_1-8)alkylamino(C_1-8)alkyl, di(C_1-8)alkylamino(C_1-8)alkyl, (C_1-8)alkanoyl; or

R¹⁰and R¹²are taken together to form —(CH₂)_n—, wherein s is 0 (a bond) or 1 to 4, while R¹¹and R¹³are as defined as above; or R¹⁰and R¹²are taken together to form a double bond when i is 0 and k is 1, while R¹¹and R¹³are as defined above; or R¹⁰and R¹¹are taken together to form —(CH₂)_t—, wherein t is 2 to 8, while R¹²and R¹³are as defined as above, or R¹²and R¹³are taken together to form —(CH₂)_n— wherein u is 2 to 8, while R¹⁰and R¹¹are as defined as above; or

i is from 0 to 4;

j is from 0 to 4; and

k is 0 or 1; and

R¹⁴is hydrogen or a functionality that acts as a prodrug.

69. The compound of claim 66, wherein R¹and R²independently represent hydrogen, halogen, (C_1-6)alkyl, (C_6-10)aryl, (C_6-10)ar(C_1-6)alkyl, 5-14 member heteroaryl, or 5-14 member heteroaryl(C_1-8)alkyl.

70. The compound of claim 69, wherein R¹and R²independently represent hydrogen, methyl, ethyl, propyl, butyl, phenyl, benzyl or phenylethyl.

71. The compound of claim 70, wherein R¹and R²independently represent hydrogen, methyl, ethyl or propyl.

72. The compound of claim 66, wherein R³, R⁴and R⁵independently represent hydrogen, (C_1-6)alkyl, (C_6-10)aryl, or (C_6-10)ar(C_1-6)alkyl.

73. The compound of claim 72, wherein R³, R⁴and R⁵are hydrogen or (C_1-4)alkyl.

74. The compound of claim 66, wherein R⁶, R⁷, R⁸and R⁹independently represent hydrogen, halogen or (C_1-6)alkyl.

75. The compound of claim 66, wherein X is oxygen, —CH₂— or —(C═O)NH—.

76. The compound of claim 75, wherein X is oxygen or —CH₂—.

77. The compound of claim 66, wherein:

X is —(C═O)NH—;

n, m, a and v are each 0; and

R⁶, R⁷, R¹²and R¹³are hydrogen.

78. The compound of claim 66, wherein:

X is oxygen;

n and m are each 0;

a and v are each 1;

D is oxygen;

R⁶, R⁷, R⁸and R⁹are hydrogen.

79. The compound of claim 66, wherein:

X is oxygen;

n, m and v are each 0;

a is 1; and

R⁶, R⁷, R¹²and R¹³are hydrogen.

80. The compound of claim 66, wherein:

X is —CH₂—;

n, m and v are each 0;

a is 1; and

R⁶, R⁷, R¹²and R¹³are hydrogen.

81. The compound of claim 66, wherein v is 0.

82. The compound of claim 66, wherein:

R¹is hydrogen or —CH₃;

R², R³, R⁴, R⁵, R⁶, R⁷, R⁸and R⁹are hydrogen;

X is oxygen or —CH₂—;

n is 0 or 1;

m is 0 or 1;

R¹⁰, R¹¹, R¹²and R¹³independently represent hydrogen, (C₁-C₆)alkyl or (C_6-10)ar(C_1-6)alkyl; or

one of the combination R¹⁰and R¹¹, R¹²and R¹³or R¹⁰and R¹²are taken together to form —(CH₂)_n—, wherein s is 1 while the remaining of R¹⁰—R¹³are defined above;

i is 0 or 1;

j is 0 or 1;

k is 0 or 1;

R¹⁴is hydrogen or alkyl;

W is:

wherein:

R¹⁵is hydrogen, halogen, (C_1-8)alkyl, (C_6-10)aryl or (C_6-10)aryl(C_1-8)alkyl; and

R¹⁶is hydrogen, (C_1-8)alkyl, halo(C_1-8)alkyl or halogen.

83. The compound of claim 66, which is one of:

3-{5-[3-(2-pyridylamino)propoxy]indolyl}propanoic acid;

3-{5-[3-(2-pyridylamino)propoxy]indolyl}acetic acid;

3-{2-methyl-5-[3-(2-pyridylamino)propoxy]indolyl}propanoic acid;

2-(trans-2-{5-[3-(2-pyridylamino)propoxy]indolyl}cyclopropyl)acetic acid;

3-(5-{2-[6-(methylamino)-2-pyridyl]ethoxy}indolyl)propanoic acid;

2-benzyl-3-{5-[3-(2-pyridylamino)propoxy]indolyl}propanoic acid;

2-methyl-3-{5-[3-(2-pyridylamino)propoxy]indolyl}propanoic acid;

2-({5-[3-(2-pyridylamino)propoxy]indolyl}methyl)pentanoic acid;

2-({5-[3-(2-pyridylamino)propoxy]indolyl}methyl)octanoic acid;

3-{5-[2-(6-methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-3-phenyl-propionic acid;

3-{5-[2-(6-methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-3-quinolin-3-yl-propionic acid;

3-{5-[2-(6-methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-3-pyridin-3-yl-propionic acid;

3-{5-[2-(6-methylamino-pyridin-2-yl)-ethoxy]-indol-1-yl}-hexanoic acid;

or a pharmaceutically acceptable salt, hydrate, solvate or prodrug thereof.

84. A pharmaceutical composition comprising a compound of claim 66 and a pharmaceutically acceptable carrier or diluent.

85. The compound according to claim 66, wherein R¹²and R¹³are independently selected from: