US20070017914A1 - Porous film cartridge - Google Patents
Porous film cartridge Download PDFInfo
- Publication number
- US20070017914A1 US20070017914A1 US10/575,366 US57536606A US2007017914A1 US 20070017914 A1 US20070017914 A1 US 20070017914A1 US 57536606 A US57536606 A US 57536606A US 2007017914 A1 US2007017914 A1 US 2007017914A1
- Authority
- US
- United States
- Prior art keywords
- porous membrane
- barrel
- cap
- opening edge
- sandwiching
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 239000012528 membrane Substances 0.000 claims abstract description 182
- 230000002093 peripheral effect Effects 0.000 claims abstract description 18
- 239000011324 bead Substances 0.000 claims description 15
- 238000002604 ultrasonography Methods 0.000 claims description 9
- 239000000853 adhesive Substances 0.000 claims description 5
- 230000001070 adhesive effect Effects 0.000 claims description 5
- 238000001746 injection moulding Methods 0.000 claims description 2
- 239000012778 molding material Substances 0.000 claims description 2
- 238000003466 welding Methods 0.000 description 39
- 239000007788 liquid Substances 0.000 description 33
- 150000007523 nucleic acids Chemical class 0.000 description 26
- 102000039446 nucleic acids Human genes 0.000 description 26
- 108020004707 nucleic acids Proteins 0.000 description 26
- 239000000243 solution Substances 0.000 description 14
- 238000003825 pressing Methods 0.000 description 11
- 238000001914 filtration Methods 0.000 description 9
- 239000012488 sample solution Substances 0.000 description 9
- 239000012535 impurity Substances 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 230000007547 defect Effects 0.000 description 6
- 239000002253 acid Substances 0.000 description 5
- 150000007513 acids Chemical class 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 238000011084 recovery Methods 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000011534 wash buffer Substances 0.000 description 4
- 229920002284 Cellulose triacetate Polymers 0.000 description 3
- SMEGJBVQLJJKKX-HOTMZDKISA-N [(2R,3S,4S,5R,6R)-5-acetyloxy-3,4,6-trihydroxyoxan-2-yl]methyl acetate Chemical compound CC(=O)OC[C@@H]1[C@H]([C@@H]([C@H]([C@@H](O1)O)OC(=O)C)O)O SMEGJBVQLJJKKX-HOTMZDKISA-N 0.000 description 3
- NNLVGZFZQQXQNW-ADJNRHBOSA-N [(2r,3r,4s,5r,6s)-4,5-diacetyloxy-3-[(2s,3r,4s,5r,6r)-3,4,5-triacetyloxy-6-(acetyloxymethyl)oxan-2-yl]oxy-6-[(2r,3r,4s,5r,6s)-4,5,6-triacetyloxy-2-(acetyloxymethyl)oxan-3-yl]oxyoxan-2-yl]methyl acetate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](OC(C)=O)[C@H]1OC(C)=O)O[C@H]1[C@@H]([C@@H](OC(C)=O)[C@H](OC(C)=O)[C@@H](COC(C)=O)O1)OC(C)=O)COC(=O)C)[C@@H]1[C@@H](COC(C)=O)O[C@@H](OC(C)=O)[C@H](OC(C)=O)[C@H]1OC(C)=O NNLVGZFZQQXQNW-ADJNRHBOSA-N 0.000 description 3
- 229940081735 acetylcellulose Drugs 0.000 description 3
- 229920002301 cellulose acetate Polymers 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000000465 moulding Methods 0.000 description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 229920004890 Triton X-100 Polymers 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 239000000155 melt Substances 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- -1 polytetrafluoroethylene Polymers 0.000 description 2
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 2
- 239000004810 polytetrafluoroethylene Substances 0.000 description 2
- 238000007670 refining Methods 0.000 description 2
- 230000000630 rising effect Effects 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Natural products C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 description 1
- 239000007984 Tris EDTA buffer Substances 0.000 description 1
- 238000003848 UV Light-Curing Methods 0.000 description 1
- 235000010724 Wisteria floribunda Nutrition 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000003795 desorption Methods 0.000 description 1
- 229920005994 diacetyl cellulose Polymers 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229960000789 guanidine hydrochloride Drugs 0.000 description 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
- 239000010800 human waste Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229920000620 organic polymer Polymers 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 239000004014 plasticizer Substances 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000379 polypropylene carbonate Polymers 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000009751 slip forming Methods 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 229920005992 thermoplastic resin Polymers 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
- B01D29/01—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with flat filtering elements
- B01D29/012—Making filtering elements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
- B01D29/085—Funnel filters; Holders therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/147—Microfiltration
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
- B01D63/08—Flat membrane modules
- B01D63/081—Manufacturing thereof
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D65/00—Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D65/00—Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
- B01D65/003—Membrane bonding or sealing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2313/00—Details relating to membrane modules or apparatus
- B01D2313/02—Specific tightening or locking mechanisms
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2313/00—Details relating to membrane modules or apparatus
- B01D2313/04—Specific sealing means
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2313/00—Details relating to membrane modules or apparatus
- B01D2313/04—Specific sealing means
- B01D2313/042—Adhesives or glues
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2313/00—Details relating to membrane modules or apparatus
- B01D2313/20—Specific housing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0689—Sealing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/12—Specific details about manufacturing devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0681—Filter
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
- B01L3/50255—Multi-well filtration
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T29/00—Metal working
- Y10T29/49—Method of mechanical manufacture
- Y10T29/49826—Assembling or joining
- Y10T29/49908—Joining by deforming
Definitions
- the present invention relates to a porous membrane cartridge used for a filtration of a liquid and the like.
- a porous membrane is widely used in a laboratory and a factory for a filtration of a liquid and an adsorption of a specific substance in the liquid. Then in using the porous membrane for such a purpose, it is requested to hold the porous membrane on the way of a passage where the liquid passes.
- the holding method is generally used a method of holding the porous membrane with sandwiching it between two members having a passage where the liquid passes.
- porous membrane Because such a porous membrane is generally used in a precise experiment and measurement, a purified one is requested and is usually changed if once used. Therefore, it is convenient in points of a purification and an expedience in use to make a porous membrane a cartridge holding the porous membrane in a state of being able to pass a liquid therethrough.
- a porous membrane As such a porous membrane is known, for example, such a nucleic acid refining unit described in Japanese Patent Laid-Open Publication No. 2002-345465.
- the nucleic acid in a case of using the conventional porous membrane in refining a nucleic acid, although the nucleic acid is adsorbed to the porous membrane, then is adsorbed to the porous membrane, and making a specific liquid flow, the nucleic acid is desorbed; however, if unable to make the liquid for desorption sufficiently flow also in corner portions of the porous membrane, there is a problem that: the nucleic acid remains in the corners and a recovery efficiency of the nucleic acid worsens.
- the present invention is made, and a purpose thereof is to provide a porous membrane cartridge for preventing a liquid from going around a side portion thereof.
- a porous membrane cartridge of the present invention is configured as follows: Namely, the porous membrane cartridge comprises a cylindrical barrel having openings at a top end and a rear end, respectively; a cap formed into a cylindrical shape having a fit-in portion fitted outside the top end, abutting with an opening edge of the top end, and having a sandwiching face for sandwiching a porous membrane between itself and the barrel; and the porous membrane sandwiched between the opening edge of the barrel and the cap, wherein the cap is fixed to the barrel so as not to be pulled out of the barrel in a state of crushing a peripheral edge of the porous membrane and sandwiching the porous membrane between itself and the cap.
- an opening edge of a barrel and a sandwiching face of a cap are sandwiched in a state of a peripheral edge of the porous membrane being crushed.
- the porous membrane has a porosity, it is strongly sandwiched by the sandwiching face between the opening edge of the barrel and the sandwiching face of the cap, the porosity is crushed as if occluded, and a liquid results in not flowing therefrom.
- the cap is fixed to the barrel in this state, the liquid does not go around a side portion of the porous membrane. Accordingly, when using the porous membrane cartridge in filtration and adsorption, it is enabled to prevent a discharged liquid from being contaminated by a liquid going around the side portion of the porous membrane.
- the cap to the barrel can be used any one of a fit-in, adhesion, ultrasonic welding, laser welding, insert molding, and the like.
- the opening edge of the barrel is formed to a taper where an inner perimeter side is more retreated than an outer perimeter side
- it is preferable that the opening edge of the barrel is welded to the sandwiching face of the cap by ultrasound.
- welding can be performed by the portion of the outer perimeter and a sandwiching force of the porous membrane gradually becomes smaller as the porous membrane draws apart from the welded portion (namely, the portion of the outer perimeter), it is enabled to avoid a stress on the porous membrane from concentrating and to prevent a breakage thereof from occurring.
- the taper is continuously formed into a flat portion formed at an outmost perimeter of the opening edge, it sandwiches the porous membrane by a clear welded portion (energy director), it is enabled to reduce an unnecessary positional displacement of the porous membrane.
- a porous membrane cartridge may also be configured by: circularly forming a bead portion as a welded portion (energy director), additionally the porous membrane is sandwiched and crushed with the bead portion, and the cap and the barrel are welded by ultrasound.
- any one of the adhesion portion and welded portion of the barrel and the cap is not limited to the opening edge and the sandwiching face.
- the barrel may also have a joint portion for abutting with an opening edge of a fit-in portion of the cap on own outer perimeter face, make the cap and the barrel adhere or be welded between the opening edge and the joint portion, and thereby configure a porous membrane cartridge.
- the cap in a case of fixing the cap by fitting in the barrel, it is enabled to fix the cap to the barrel by an engagement between any one of a depression and a protrusion formed on an outer perimeter face of the barrel and any one of those formed on an inner perimeter face of the cap.
- the protrusion on the outer perimeter face of the barrel and the protrusion on the inner perimeter face of the cap may be engaged.
- a cap molded in advance and a porous membrane disposed in the cap within a cavity of an injection molding mold, to inject a molding material in the cavity, to thereby mold the barrel, and to simultaneously sandwich a porous membrane with the opening edge of the barrel and the sandwiching face of the cap.
- a porous membrane cartridge unit where a plurality of porous membrane cartridges are connected, it is enabled to align a sandwiching force of porous membranes of the plurality of the porous membrane cartridges, and while simultaneously forming a plurality of barrels, to make up a state of these barrels being stably connected.
- porous membrane cartridge of the present invention is enabled to prevent a liquid from going around a side portion of the porous membrane.
- FIG. 1 is an exploded perspective view of a porous membrane cartridge related to a first embodiment of the present invention.
- FIG. 2 is a section view of a porous membrane cartridge related to the first embodiment.
- FIG. 3 is an enlarged section perspective view of a cap related to the first embodiment.
- FIG. 4 is an enlarged view of a part A in FIG. 2 , and shows a state before a barrel 10 and a cap 20 are welded.
- FIG. 5 is drawings showing a process of assembling a porous membrane cartridge related to the first embodiment; (a) shows a state before welding; (b) shows a state of starting to crush a porous membrane F; (c) shows a state on the way of the welding; and (d) shows a state after the welding.
- FIG. 6 is section views showing a porous membrane cartridge 1 A related to a second embodiment of the present invention and corresponding to the enlarged view of the part A in FIG. 2 ; (a) shows a state before welding; (b) shows a state on the way of the welding; and (c) shows a state after the welding.
- FIG. 7 is a section view showing another example of the porous membrane cartridge 1 A related to the second embodiment and corresponding to the enlarged view of the part A in FIG. 2 .
- FIG. 8 is drawings showing a porous membrane cartridge 1 D related to a third embodiment of the present invention and corresponding to the enlarged view of the part A in FIG. 2 ; (a) shows a state before welding; and (b) shows a state after the welding.
- FIG. 9 ( a ) is another example of the porous membrane cartridge 1 D of the third embodiment; and FIG. 9 ( b ) shows still another example.
- FIG. 1 is an exploded perspective view of a porous membrane cartridge related to a first embodiment of the present invention
- FIG. 2 is a section view of a porous membrane cartridge related to the first embodiment
- FIG. 3 is an enlarged section perspective view of a cap related to the first embodiment.
- a porous membrane cartridge 1 related to the first embodiment is configured of a porous membrane F, and a barrel 10 and a cap 20 for holding the porous membrane F and forming a passage where a liquid passes.
- the barrel 10 comprises a cylindrical main body portion 12 and a cylindrical top end 13 continuing into the main body portion 12 , and has an opening 11 a at the top end 13 and an opening 11 b at a rear end of the main body portion 12 . Therefore, a liquid is enabled to pass from the opening 11 b to the opening 11 a .
- An outer diameter of the top end 13 is designed to be one size smaller that of the main body portion 12 . Meanwhile, in the embodiment a side of the barrel 10 and the cap 20 in which a liquid flows is called a rear end, and a side from which the liquid is pushed out is called a top end.
- the cap 20 comprises a cylindrical fit-in portion 22 and a nozzle 23 continuing into a top side of the fit-in portion 22 .
- An opening 21 a is formed at the top end of the nozzle 23
- an opening 21 b is formed at the rear end of the fit-in portion 22 , and thus a liquid is enabled to pass from the rear end to top end of the cap 20 .
- An inner diameter of the fit-in portion 22 is formed into a diameter enabled to fit in the diameter of the top end 13 of the barrel 10 .
- radial ribs 25 (only three are shown in FIG. 3 ) at a bottom portion 26 of the fit-in portion 22 continuing into the nozzle 23 from the portion 22 .
- a sandwiching face 24 made higher by one step from the bottom portion 26 so as to be a same height as an upper face of the ribs 25 .
- the sandwiching face 24 is a face for sandwiching the porous membrane F between itself and an opening edge 14 (see FIG. 1 ) corresponding to an end edge of the opening 11 a of the barrel 10 .
- the ribs 25 are formed at the same height as the sandwiching face 24 , thereby support the porous membrane F disposed at the bottom portion 26 within the cap 20 , and prevent the porous membrane F from elongating and breaking by a liquid flowing into the top end (opening 21 a ) from the rear end (opening 21 b ).
- the ribs 25 are radially formed, and thereby, it is designed that a liquid smoothly flows in the nozzle 23 in being made to flow from the rear end to the top end.
- the barrel 10 and the cap 20 are composed of polystyrene, they are not limited thereto.
- a thermoplastic resin is available that can be welded by ultrasound.
- a material is available that can be made to adhere by adhesive.
- the porous membrane F is composed of an organic polymer and is formed into a circle of which a diameter approximately matches the inner diameter of the cap 20 and the outer diameter of the top end 13 of the barrel 10 .
- a material of the porous membrane F for example, a surface-saponified substance of acetylcellulose is suitable.
- acetylcellulose although any one of mono acetylcellulose, diacetylcellulose, and triacetylcellulose is available, specifically triacetylcellulose is preferable.
- a porous membrane of such PTFE polytetrafluoroethylene
- polyamide polyamide
- polypropylene polypropylene
- polycarbonate a porous membrane of such PTFE (polytetrafluoroethylene), polyamide, polypropylene, and polycarbonate.
- FIG. 4 is an enlarged view of a part A in FIG. 2 .
- a slight portion (for example, a degree of 0.1 mm width) from an outmost perimeter is formed as a flat portion 14 a perpendicular to an axial line (longitudinal direction) of the barrel 10 , and continuing into the flat portion 14 a is formed a taper portion 14 b that becomes an inside taper that gradually retreats from an outer perimeter toward an inner perimeter thereof.
- the taper portion 14 b makes a small angle of 3 to 20 degrees, for example, 10 degrees with the flat portion 14 a .
- a diameter of an end 14 c at an inner perimeter side of the taper portion 14 b approximately matches that of an end 24 a at an inner perimeter side of the sandwiching face 24 , and from the end 14 c across the inner perimeter face 13 a of the barrel 10 , a chamfer 15 is formed that makes an angle of 60 degrees with the flat portion 14 a.
- a longitudinal direction distance of the barrel 10 that retreats from the flat portion 14 a to the end 14 c so as to be a same degree as a thickness of the porous membrane F and so that the end 14 c abuts with a surface of the membrane F after welding the barrel 10 and the cap 20 .
- the width of the flat portion 14 a is preferably 0.02 to 1 mm. This is because a filtration effective area decreases if the width of the flat portion 14 a is made too wide.
- the width of the flat portion 14 a is preferably 0.02 to 0.5 mm, and more preferably 0.02 to 0.2 mm.
- FIG. 4 and FIG. 5 showing an assembling process of the porous membrane cartridge 1 .
- the porous membrane F As shown in FIG. 4 , dispose the porous membrane F so as to be put on the bottom portion 26 of the cap 20 . At this time, because the sandwiching face 24 and the ribs 25 are designed higher than the bottom portion 26 by one step, the porous membrane F is, to be more precise, put on the sandwiching face 24 and the ribs 25 . Then because the diameter of the porous membrane F is approximately same as the inner diameter of the cap 20 , as shown in FIG. 5 ( a ), a peripheral edge Fa of the porous membrane F results in being positioned on the sandwiching face 24 .
- the porous membrane cartridge 1 thus made is used as follows:
- sample solutions containing nucleic acids prepare body fluids such as a whole blood, plasma, serum, urine, human waste, semen, and saliva taken as analytes; or solutions adjusted from biotic materials such as a soluble matter and homogenate of a vegetable (or its part), an animal (or its part), and the like. Treat these solutions with a water solution containing a reagent, which solves a cell membrane and solublizes the nucleic acids. Thus the cell membrane and nucleic membrane are solved, and the nucleic acids are dispersed in the water solution.
- body fluids such as a whole blood, plasma, serum, urine, human waste, semen, and saliva taken as analytes; or solutions adjusted from biotic materials such as a soluble matter and homogenate of a vegetable (or its part), an animal (or its part), and the like.
- a water solution containing a reagent which solves a cell membrane and solublizes the nucleic acids.
- a sample is a whole blood
- red blood cells and various proteins are removed and white blood cells and nucleic membranes are solved by incubation of 10 minutes at 60 degrees Celsius in a state of addition of Guanidine Hydrochloride, Triton-X100, and Protease K (manufactured by SIGMA Corp.).
- a sample solution is completed. Pass the sample solution while applying a pressure from the opening 11 b at the rear end side of the barrel 10 toward the opening 21 a of the top of the nozzle 23 .
- a pressure from the opening 11 b at the rear end side of the barrel 10 toward the opening 21 a of the top of the nozzle 23 is applied.
- the nucleic acids in the sample solution are adsorbed to the porous membrane F.
- the nucleic acid washing buffer has a composition of desorbing not nucleic acids adsorbed to the porous membrane F but impurities, it is composed of a water solution containing a main agent and a buffer agent, and a surfactant as needed.
- a main agent a solution containing ethanol, Tris, and Triton-X100 is preferable.
- the porous membrane cartridge 1 of the embodiment because when passing such a sample solution where nucleic acids is dispersed, a nucleic acid washing buffer, and refined distilled water, they do not go around a side portion of the porous membrane F, it does not occur that: the acids are discharged without being adsorbed to the membrane F, and impurities mix in a solution where the acids are recovered.
- the end 14 c is in contact with the porous membrane F and it does not also occur that the various liquids described above enter a space between the membrane F and the opening edge 14 of the barrel 10 , impurities do not mix in a nucleic acid solution after the recovery, and a nucleic acid recovery efficiency is also higher.
- part of the opening edge 14 is formed so as to be the taper portion 14 b where an inner perimeter side is more retreated than an outer perimeter side and a sandwiching force by the opening edge 14 and the sandwiching face 24 gradually becomes smaller from an outer perimeter to an inner perimeter, it is enabled to avoid a concentration of a stress applied to the porous membrane F.
- the opening edge 14 is designed to be a blunt angle also alleviates the concentration of the stress applied to the porous membrane F, it is enabled to prevent the membrane F from breaking.
- the first embodiment thus described is one example of the present invention, and because it suffices that the invention may fix the barrel 10 and the cap 20 in a state of crushing and sandwiching the porous membrane F, various variation examples as below can be considered. Meanwhile, in embodiments below with respect to a same portion as in the first embodiment, a detailed description thereof will be omitted, appending a same symbol.
- FIG. 6 is drawings showing a porous membrane cartridge 1 A related to a second embodiment of the present invention, and is section views corresponding to the enlarged view of the part A in FIG. 2 .
- a bead 16 a of which a section is chevron is provided across a whole perimeter in the vicinity of an outer perimeter of an opening edge 16 of the barrel 10 .
- an inner perimeter side of the bead 16 a is designed to be a flat portion 16 b for orthogonalizing an axial line of the barrel 10 .
- the bead 16 a melts and penetrates the porous membrane F, and as shown in FIG. 6 ( b ), the sandwiching face 24 and the bead 16 a are welded. If further making the welding proceed, the flat portion 16 b crushes and sandwiches the porous membrane F between itself and the sandwiching face 24 as shown in FIG. 6 ( c ).
- porous membrane cartridge 1 A thus made, because the opening edge 16 and the sandwiching face 24 are welded while the peripheral edge Fa of the porous membrane F melts, a liquid does not go around a side portion of the porous membrane F. In addition, because the porous membrane F is sandwiched between the flat portion 16 b and the sandwiching face 24 while crushed between them, a liquid does not enter the portion Fa of the membrane F.
- a recovery efficiency of nucleic acids is better and mixing of impurities in a solution after a recovery is less.
- FIG. 8 is drawings showing a porous membrane cartridge 1 D related to a third embodiment of the present invention and corresponding to the enlarged view of the part A in FIG. 2 .
- the porous membrane cartridge 1 D is a mode of welding the barrel 10 and the cap 20 at a portion of an outer perimeter more than the sandwiching face 24 .
- a step portion 27 for welding defined by: a wall face portion 27 a perpendicularly rising up with respect to the sandwiching face 24 at outside of the sandwiching face 24 ; and a slant portion 27 b slantedly rising up toward the inner perimeter of the fit-in portion 22 from an upper end (namely, the rear end) in FIG. 8 ( a ) of the wall face portion 27 a.
- a flat portion 17 a parallel with the sandwiching face 24 formed at an inner perimeter side a wall face portion 17 b composed of a peripheral face for fitting in the wall face portion 27 a formed with continuing from an outer perimeter end of the flat portion 17 a ; a slant portion 17 c formed with continuing from the top end in FIG. 8 ( a ) of the wall face portion 27 a and parallel with the slant portion 27 b ; and a flat portion 17 d formed with continuing into the slant portion 17 c and extending toward the outer perimeter of the barrel 10 .
- porous membrane cartridge 1 D sandwiches the porous membrane F between the sandwiching face 24 and the flat portion 17 a , and the barrel 10 and the cap 20 is applied to an ultrasonic welder, the flat portion 17 d abuts with the slant portion 27 b and the abutment portion is welded. Then the peripheral edge Fa of the porous membrane F is sandwiched between the flat portion 17 a and the sandwiching face 24 in a state of being crushed.
- FIG. 9 ( a ) it is also enabled to make the main body 12 of the barrel 10 and a face of a step difference of an outer perimeter face of the top end 13 a joint portion 18 for welding, on the other hand to form a protrusion abutting with the portion 18 across a whole perimeter at an opening edge 28 at a rear end side of the cap 20 , and to thus make a porous membrane cartridge 1 E where the joint portion 18 and the opening edge 28 are welded.
- the barrel 10 and the cap 20 can also be made to adhere by any one of an adhesive and a UV curing resin, and the outer perimeter face of the top end 13 of the barrel 10 and the inner perimeter face of the fit-in portion 22 of the cap 20 can also be welded by laser. Meanwhile, in a case of laser welding, it is preferable to make the barrel 10 a material that can generate heat by absorbing laser or to blend a plasticizer in the barrel 10 .
- the barrel 10 and the cap 20 As a material of the barrel 10 and the cap 20 was used polystyrene (manufactured by A&M Styrene Co., Ltd.).
- the outer diameter of the opening edge 14 of the barrel 10 was made 7 mm
- the width of the flat portion 14 a at outer perimeter side of opening edge 14 was made 0.05 mm
- the angle of the taper portion 14 b was formed to be 10 degrees for the flat portion 14 a.
- porous membrane F triacetylcellulose (Microfilter FM500 manufactured by Fuji Photo Film Co., Ltd.).
- an ultrasonic welder 40 KHz, 800 KW manufactured by Branson Corp. as an ultrasonic welder, disposing the porous membrane F at the bottom portion 26 of the cap 20 same as in the first embodiment, and fitting the top end 13 of the barrel 10 in the cap 20 , the barrel 10 was vibrated while pressed toward the cap 20 by a horn of the ultrasonic welder. Changing a pressing force and a vibration time at this time, a welding result under each condition was determined.
- Table 1 shows the result.
- the symbol x indicates that the barrel 10 and the cap 20 could not be welded; the symbol ⁇ indicates that although the barrel 10 and the cap 20 could be welded, the welding was insufficient and there was a liquid leakage from the welded portion; the symbol ⁇ indicates that there was a defect that the porous membrane F was perforated or a formed product was deformed; and the symbol O indicates that the welding could be performed well without the defect.
- the welding could not be performed in a case of the vibration time not more than 0.1 sec and could be performed well in a case of the vibration time between 0.15 sec and 0.45 sec; in a case of the vibration time not less than 0.5 sec, a welding time was too long and there was the defect in a formed product.
- the welding could not be performed in a case of the vibration time of 0.03 sec and could be performed well in a case of the vibration time between 0.05 sec and 0.45 sec; in a case of the vibration time not less than 0.5 sec, a welding time was too long and there was the defect in a formed product.
- the welding could not be performed in a case of the vibration time of 0.03 sec and could be performed well in a case of the vibration time between 0.05 sec and 0.35 sec; in a case of the vibration time not less than 0.4 sec, a welding time was too long and there was the defect in a formed product.
- the welding could not be performed in a case of the vibration time of 0.03 sec and could be performed well in a case of the vibration time between 0.05 sec and 0.25 sec; in a case of the vibration time not less than 0.3 sec, a welding time was too long and there was the defect in a formed product.
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Water Supply & Treatment (AREA)
- Analytical Chemistry (AREA)
- Manufacturing & Machinery (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Closures For Containers (AREA)
Abstract
Description
- The present invention relates to a porous membrane cartridge used for a filtration of a liquid and the like.
- A porous membrane is widely used in a laboratory and a factory for a filtration of a liquid and an adsorption of a specific substance in the liquid. Then in using the porous membrane for such a purpose, it is requested to hold the porous membrane on the way of a passage where the liquid passes. As the holding method is generally used a method of holding the porous membrane with sandwiching it between two members having a passage where the liquid passes.
- Because such a porous membrane is generally used in a precise experiment and measurement, a purified one is requested and is usually changed if once used. Therefore, it is convenient in points of a purification and an expedience in use to make a porous membrane a cartridge holding the porous membrane in a state of being able to pass a liquid therethrough. As such a porous membrane is known, for example, such a nucleic acid refining unit described in Japanese Patent Laid-Open Publication No. 2002-345465.
- However, in a conventional porous membrane there is a fear that a liquid to pass the porous membrane goes around a side thereof. Particularly, in a case that two components for sandwiching the porous membrane are fixed only by fastening with fit-in, a size changes according to an expansion by a temperature change and swelling by a humidity change, and a sandwiched state changes in some case. In addition, in a case of using the conventional porous membrane in refining a nucleic acid, although the nucleic acid is adsorbed to the porous membrane, then is adsorbed to the porous membrane, and making a specific liquid flow, the nucleic acid is desorbed; however, if unable to make the liquid for desorption sufficiently flow also in corner portions of the porous membrane, there is a problem that: the nucleic acid remains in the corners and a recovery efficiency of the nucleic acid worsens. In view of such a problem, the present invention is made, and a purpose thereof is to provide a porous membrane cartridge for preventing a liquid from going around a side portion thereof.
- In order to solve the problem, a porous membrane cartridge of the present invention is configured as follows: Namely, the porous membrane cartridge comprises a cylindrical barrel having openings at a top end and a rear end, respectively; a cap formed into a cylindrical shape having a fit-in portion fitted outside the top end, abutting with an opening edge of the top end, and having a sandwiching face for sandwiching a porous membrane between itself and the barrel; and the porous membrane sandwiched between the opening edge of the barrel and the cap, wherein the cap is fixed to the barrel so as not to be pulled out of the barrel in a state of crushing a peripheral edge of the porous membrane and sandwiching the porous membrane between itself and the cap.
- In accordance with such a porous membrane cartridge, an opening edge of a barrel and a sandwiching face of a cap are sandwiched in a state of a peripheral edge of the porous membrane being crushed. In other words, if because the porous membrane has a porosity, it is strongly sandwiched by the sandwiching face between the opening edge of the barrel and the sandwiching face of the cap, the porosity is crushed as if occluded, and a liquid results in not flowing therefrom. Then because in the porous membrane cartridge the cap is fixed to the barrel in this state, the liquid does not go around a side portion of the porous membrane. Accordingly, when using the porous membrane cartridge in filtration and adsorption, it is enabled to prevent a discharged liquid from being contaminated by a liquid going around the side portion of the porous membrane.
- As a method of fixing the cap to the barrel can be used any one of a fit-in, adhesion, ultrasonic welding, laser welding, insert molding, and the like. To be more precise, it is preferable that the opening edge of the barrel and the sandwiching face of the cap are welded by ultrasound.
- Then, in addition that the opening edge of the barrel is formed to a taper where an inner perimeter side is more retreated than an outer perimeter side, it is preferable that the opening edge of the barrel is welded to the sandwiching face of the cap by ultrasound. Thus because in addition to crushing a peripheral edge of the porous membrane by a portion of an outer perimeter side, welding can be performed by the portion of the outer perimeter and a sandwiching force of the porous membrane gradually becomes smaller as the porous membrane draws apart from the welded portion (namely, the portion of the outer perimeter), it is enabled to avoid a stress on the porous membrane from concentrating and to prevent a breakage thereof from occurring. At this time, because if the taper is continuously formed into a flat portion formed at an outmost perimeter of the opening edge, it sandwiches the porous membrane by a clear welded portion (energy director), it is enabled to reduce an unnecessary positional displacement of the porous membrane.
- In this case, in addition to sandwiching and crushing the porous membrane between the outer perimeter side of the opening edge and the sandwiching face, it is preferable that a portion of the outer perimeter side and that of the sandwiching face are welded therebetween by ultrasound as it is.
- In addition, a porous membrane cartridge may also be configured by: circularly forming a bead portion as a welded portion (energy director), additionally the porous membrane is sandwiched and crushed with the bead portion, and the cap and the barrel are welded by ultrasound.
- In addition, any one of the adhesion portion and welded portion of the barrel and the cap is not limited to the opening edge and the sandwiching face. For example, the barrel may also have a joint portion for abutting with an opening edge of a fit-in portion of the cap on own outer perimeter face, make the cap and the barrel adhere or be welded between the opening edge and the joint portion, and thereby configure a porous membrane cartridge. Thus, if sandwiching a porous membrane and jointing a barrel and a cap at separate portions, it does not occur to damage the porous membrane even in a case of using an especially delicate porous membrane.
- Furthermore, in a case of fixing the cap by fitting in the barrel, it is enabled to fix the cap to the barrel by an engagement between any one of a depression and a protrusion formed on an outer perimeter face of the barrel and any one of those formed on an inner perimeter face of the cap. At this time, not to mention an engagement between the depression on the outer perimeter face of the barrel and the protrusion on the inner perimeter face of the cap and an engagement between the protrusion on the outer perimeter face of the barrel and the depression on the inner perimeter face of the cap, the protrusion on the outer perimeter face of the barrel and the protrusion on the inner perimeter face of the cap may be engaged.
- In addition, not limiting to the portions described above, it is also enabled to fix the cap and the barrel by any one of an adhesive and laser welding on a fit-in face where the outer perimeter face of the barrel and the inner perimeter face of the cap oppose each other.
- In addition, in a case of performing molding by insert molding, it is preferable to place a cap molded in advance and a porous membrane disposed in the cap within a cavity of an injection molding mold, to inject a molding material in the cavity, to thereby mold the barrel, and to simultaneously sandwich a porous membrane with the opening edge of the barrel and the sandwiching face of the cap. Thus it is enabled to realize a sandwich and fixation of the porous membrane. In addition, in a case of making a porous membrane cartridge unit where a plurality of porous membrane cartridges are connected, it is enabled to align a sandwiching force of porous membranes of the plurality of the porous membrane cartridges, and while simultaneously forming a plurality of barrels, to make up a state of these barrels being stably connected.
- In accordance with a porous membrane cartridge of the present invention thus described, it is enabled to prevent a liquid from going around a side portion of the porous membrane.
-
FIG. 1 is an exploded perspective view of a porous membrane cartridge related to a first embodiment of the present invention. -
FIG. 2 is a section view of a porous membrane cartridge related to the first embodiment. -
FIG. 3 is an enlarged section perspective view of a cap related to the first embodiment. -
FIG. 4 is an enlarged view of a part A inFIG. 2 , and shows a state before abarrel 10 and acap 20 are welded. -
FIG. 5 is drawings showing a process of assembling a porous membrane cartridge related to the first embodiment; (a) shows a state before welding; (b) shows a state of starting to crush a porous membrane F; (c) shows a state on the way of the welding; and (d) shows a state after the welding. -
FIG. 6 is section views showing a porous membrane cartridge 1A related to a second embodiment of the present invention and corresponding to the enlarged view of the part A inFIG. 2 ; (a) shows a state before welding; (b) shows a state on the way of the welding; and (c) shows a state after the welding. -
FIG. 7 is a section view showing another example of the porous membrane cartridge 1A related to the second embodiment and corresponding to the enlarged view of the part A inFIG. 2 . -
FIG. 8 is drawings showing aporous membrane cartridge 1D related to a third embodiment of the present invention and corresponding to the enlarged view of the part A inFIG. 2 ; (a) shows a state before welding; and (b) shows a state after the welding. -
FIG. 9 (a) is another example of theporous membrane cartridge 1D of the third embodiment; andFIG. 9 (b) shows still another example. - Next will be described an embodiment of the present invention, referring to drawings as needed.
FIG. 1 is an exploded perspective view of a porous membrane cartridge related to a first embodiment of the present invention;FIG. 2 is a section view of a porous membrane cartridge related to the first embodiment; andFIG. 3 is an enlarged section perspective view of a cap related to the first embodiment. - Meanwhile, although in the embodiment is described a case of using a porous membrane cartridge in a nucleic acid as an application thereof, the application is not limited thereto.
- As shown in
FIG. 1 , a porous membrane cartridge 1 related to the first embodiment is configured of a porous membrane F, and abarrel 10 and acap 20 for holding the porous membrane F and forming a passage where a liquid passes. - The
barrel 10 comprises a cylindricalmain body portion 12 and a cylindricaltop end 13 continuing into themain body portion 12, and has anopening 11 a at thetop end 13 and an opening 11 b at a rear end of themain body portion 12. Therefore, a liquid is enabled to pass from theopening 11 b to theopening 11 a. An outer diameter of thetop end 13 is designed to be one size smaller that of themain body portion 12. Meanwhile, in the embodiment a side of thebarrel 10 and thecap 20 in which a liquid flows is called a rear end, and a side from which the liquid is pushed out is called a top end. - The
cap 20 comprises a cylindrical fit-inportion 22 and anozzle 23 continuing into a top side of the fit-inportion 22. - An
opening 21 a is formed at the top end of thenozzle 23, an opening 21 b is formed at the rear end of the fit-inportion 22, and thus a liquid is enabled to pass from the rear end to top end of thecap 20. - An inner diameter of the fit-in
portion 22 is formed into a diameter enabled to fit in the diameter of thetop end 13 of thebarrel 10. - Then as shown in
FIG. 2 , by fitting thetop end 13 of thebarrel 10 in the fit-inportion 22 of thecap 20 in a state of putting in the porous membrane F into theportion 22 of thecap 20, it is enabled to sandwich the porous membrane F between thecap 20 and thebarrel 10. - As shown in
FIG. 3 , in thecap 20 are formed six radial ribs 25 (only three are shown inFIG. 3 ) at abottom portion 26 of the fit-inportion 22 continuing into thenozzle 23 from theportion 22. In addition, at an outer perimeter edge of thebottom portion 26, across a whole perimeter thereof is formed a sandwichingface 24 made higher by one step from thebottom portion 26 so as to be a same height as an upper face of theribs 25. - The
sandwiching face 24 is a face for sandwiching the porous membrane F between itself and an opening edge 14 (seeFIG. 1 ) corresponding to an end edge of theopening 11 a of thebarrel 10. - The
ribs 25 are formed at the same height as thesandwiching face 24, thereby support the porous membrane F disposed at thebottom portion 26 within thecap 20, and prevent the porous membrane F from elongating and breaking by a liquid flowing into the top end (opening 21 a) from the rear end (opening 21 b). In addition, theribs 25 are radially formed, and thereby, it is designed that a liquid smoothly flows in thenozzle 23 in being made to flow from the rear end to the top end. - Meanwhile, although the
barrel 10 and thecap 20 are composed of polystyrene, they are not limited thereto. In a case of fixing thebarrel 10 and thecap 20 by ultrasonic welding, a thermoplastic resin is available that can be welded by ultrasound. In addition, in a case of fixing thebarrel 10 and thecap 20 by adhesive, a material is available that can be made to adhere by adhesive. - The porous membrane F is composed of an organic polymer and is formed into a circle of which a diameter approximately matches the inner diameter of the
cap 20 and the outer diameter of thetop end 13 of thebarrel 10. As a material of the porous membrane F, for example, a surface-saponified substance of acetylcellulose is suitable. As acetylcellulose, although any one of mono acetylcellulose, diacetylcellulose, and triacetylcellulose is available, specifically triacetylcellulose is preferable. - Meanwhile, as a general filter can also be used a porous membrane of such PTFE (polytetrafluoroethylene), polyamide, polypropylene, and polycarbonate.
-
FIG. 4 is an enlarged view of a part A inFIG. 2 . - As shown in
FIG. 4 , in the openingedge 14 of thebarrel 10, a slight portion (for example, a degree of 0.1 mm width) from an outmost perimeter is formed as aflat portion 14 a perpendicular to an axial line (longitudinal direction) of thebarrel 10, and continuing into theflat portion 14 a is formed ataper portion 14 b that becomes an inside taper that gradually retreats from an outer perimeter toward an inner perimeter thereof. Thetaper portion 14 b makes a small angle of 3 to 20 degrees, for example, 10 degrees with theflat portion 14 a. A diameter of anend 14 c at an inner perimeter side of thetaper portion 14 b approximately matches that of anend 24 a at an inner perimeter side of the sandwichingface 24, and from theend 14 c across the inner perimeter face 13 a of thebarrel 10, achamfer 15 is formed that makes an angle of 60 degrees with theflat portion 14 a. - In addition, it is preferable to set a longitudinal direction distance of the
barrel 10 that retreats from theflat portion 14 a to theend 14 c so as to be a same degree as a thickness of the porous membrane F and so that theend 14 c abuts with a surface of the membrane F after welding thebarrel 10 and thecap 20. - Although such an outer diameter of the opening
edge 14 of thebarrel 10 is decided according to a process liquid amount and a filtration time, in a case of fixing thebarrel 10 and thecap 20 by ultrasonic welding, it is preferable to press theflat portion 14 a to the porous membrane F and vibrate theportion 14 a, and to thereby set a width of theportion 14 a to a size suitable for the membrane F melting. For example, in a case that the outer diameter of the openingedge 14 is 7 mm, the width of theflat portion 14 a is preferably 0.02 to 1 mm. This is because a filtration effective area decreases if the width of theflat portion 14 a is made too wide. Furthermore, considering a manufacturing adequacy such as an energy added to thebarrel 10 in welding, the width of theflat portion 14 a is preferably 0.02 to 0.5 mm, and more preferably 0.02 to 0.2 mm. - Next will be described an assembling method of the porous membrane F, the
barrel 10, and thecap 20 thus described, referring toFIG. 4 andFIG. 5 showing an assembling process of the porous membrane cartridge 1. - As shown in
FIG. 4 , dispose the porous membrane F so as to be put on thebottom portion 26 of thecap 20. At this time, because thesandwiching face 24 and theribs 25 are designed higher than thebottom portion 26 by one step, the porous membrane F is, to be more precise, put on the sandwichingface 24 and theribs 25. Then because the diameter of the porous membrane F is approximately same as the inner diameter of thecap 20, as shown inFIG. 5 (a), a peripheral edge Fa of the porous membrane F results in being positioned on the sandwichingface 24. - Then fitting the
top end 13 of thebarrel 10 inside the fit-inportion 22 of thecap 20, make the openingedge 14 of thebarrel 10 abut with the peripheral edge Fa of the porous membrane F. In such a temporal assembly, perform welding by an ultrasonic welder not shown. For example, set thecap 20 on a platform for pressurizing of the ultrasonic welder, apply an ultrasound while pressurizing thebarrel 10 toward thecap 20 by horn. - Whereat, by pressing the
barrel 10 to thecap 20, an air gap of the porous membrane F is crushed, and then as shown inFIG. 5 (b), the peripheral edge Fa of the outmost perimeter of the porous membrane F is crushed. At this time, because theflat portion 14 a provided at the outmost perimeter of the openingedge 14 sandwiches the porous membrane F between itself and the sandwichingface 24, a position of the membrane F is decided, and an unnecessary positional displacement of the membrane F is prevented. Then part of the porous membrane F is further crushed as shown inFIG. 5 (c) while melting, and the openingedge 14 abuts with the sandwichingface 24. Subsequently, a peripheral edge including theflat portion 14 a of the openingedge 14 and that of the sandwichingface 24 melt and are welded. As a result, as shown inFIG. 5 (d), the openingedge 14 of thebarrel 10 and the sandwichingface 24 of thecap 20 sandwich the porous membrane F, and the membrane F is held in a state of its peripheral edge Fa being crushed. Also the porous membrane F existing between thetaper portion 14 b of the openingedge 14 and the sandwichingface 24 is also compressed by thetaper portion 14 b and part of an air gap is crushed. Then theend 14 c is in contact with the surface of the porous membrane F. At this time, because theend 14 c is formed as a blunt angle by thechamfer 15, it is difficult to damage the porous membrane F. - The porous membrane cartridge 1 thus made is used as follows:
- Firstly, as sample solutions containing nucleic acids, prepare body fluids such as a whole blood, plasma, serum, urine, human waste, semen, and saliva taken as analytes; or solutions adjusted from biotic materials such as a soluble matter and homogenate of a vegetable (or its part), an animal (or its part), and the like. Treat these solutions with a water solution containing a reagent, which solves a cell membrane and solublizes the nucleic acids. Thus the cell membrane and nucleic membrane are solved, and the nucleic acids are dispersed in the water solution. For example, when a sample is a whole blood, red blood cells and various proteins are removed and white blood cells and nucleic membranes are solved by incubation of 10 minutes at 60 degrees Celsius in a state of addition of Guanidine Hydrochloride, Triton-X100, and Protease K (manufactured by SIGMA Corp.).
- Adding a water soluble organic solvent, for example, ethanol to a water solution where nucleic acids are thus dispersed, a sample solution is completed. Pass the sample solution while applying a pressure from the
opening 11 b at the rear end side of thebarrel 10 toward the opening 21 a of the top of thenozzle 23. Thus the nucleic acids in the sample solution are adsorbed to the porous membrane F. - In a pressurizing system of passing a sample solution by applying the pressure with being compared to a centrifugal separation system that passes the solution by centrifugal force, although the solution attempts to flow toward the peripheral edge Fa of the porous membrane F, because in the portion Fa of the porous membrane F the
end portion 14 c of thebarrel 10 is in contact with the surface of the membrane F, the solution cannot enter a space between the membrane F and the openingedge 14 of thebarrel 10. In addition, because in the peripheral edge Fa of the porous membrane F an air gap is crushed by thetaper portion 14 b and the sandwichingface 24 and furthermore the outer perimeter portion thereof is completely closed by welding of thebarrel 10 and thecap 20, it does not occur that a sample solution goes around a side portion (edge of the outer perimeter) of the membrane F. Accordingly, nucleic acids in a sample solution result in being adsorbed only to an inside portion surrounded by theend 14 c of thebarrel 10 out of the porous membrane F. - Next, pass a nucleic acid washing buffer while applying a pressure from the
opening 11 b at the rear end side of the porous membrane cartridge 1 toward the opening 21 a of thenozzle 23. Although the nucleic acid washing buffer has a composition of desorbing not nucleic acids adsorbed to the porous membrane F but impurities, it is composed of a water solution containing a main agent and a buffer agent, and a surfactant as needed. As the main agent, a solution containing ethanol, Tris, and Triton-X100 is preferable. By this operation, impurities other than nucleic acids are removed from the porous membrane F. - At this time, out of the porous membrane F, because a nucleic acid washing buffer sufficiently passes a portion where the sample solution passed, that is, a portion surrounded by the
end 14 c, impurities are removed without remaining at the peripheral edge Fa of the membrane F. - Next, passing any one of such refined distilled water and a TE buffer (containing Tris and EDTA (ethylenediamine tetraacetic acid)) while applying a pressure from the
opening 11 b toward the opening 21 a (seeFIG. 2 ), desorb nucleic acids from the porous membrane F, desorb the acids and make them flow out, and recover a solution containing the flowed-out nucleic acids. At this time because same as in adsorbing nucleic acids to the porous membrane F, such refined distilled water sufficiently passes through a portion where the acids surrounded by theend 14 c out of the porous membrane F, the acids are sufficiently desorbed without remaining at the peripheral edge Fa of the membrane F. - Thus in the porous membrane cartridge 1 of the embodiment, because when passing such a sample solution where nucleic acids is dispersed, a nucleic acid washing buffer, and refined distilled water, they do not go around a side portion of the porous membrane F, it does not occur that: the acids are discharged without being adsorbed to the membrane F, and impurities mix in a solution where the acids are recovered. In addition, because the
end 14 c is in contact with the porous membrane F and it does not also occur that the various liquids described above enter a space between the membrane F and the openingedge 14 of thebarrel 10, impurities do not mix in a nucleic acid solution after the recovery, and a nucleic acid recovery efficiency is also higher. - In addition, in the porous membrane cartridge 1 of the embodiment, because part of the opening
edge 14 is formed so as to be thetaper portion 14 b where an inner perimeter side is more retreated than an outer perimeter side and a sandwiching force by the openingedge 14 and the sandwichingface 24 gradually becomes smaller from an outer perimeter to an inner perimeter, it is enabled to avoid a concentration of a stress applied to the porous membrane F. In addition, because that the openingedge 14 is designed to be a blunt angle also alleviates the concentration of the stress applied to the porous membrane F, it is enabled to prevent the membrane F from breaking. - In addition, in a case of using the porous membrane cartridge 1 in filtration, because a liquid does not go around the side portion of the porous membrane F, mixing of impurities in a liquid after the filtration becomes less.
- The first embodiment thus described is one example of the present invention, and because it suffices that the invention may fix the
barrel 10 and thecap 20 in a state of crushing and sandwiching the porous membrane F, various variation examples as below can be considered. Meanwhile, in embodiments below with respect to a same portion as in the first embodiment, a detailed description thereof will be omitted, appending a same symbol. -
FIG. 6 is drawings showing a porous membrane cartridge 1A related to a second embodiment of the present invention, and is section views corresponding to the enlarged view of the part A inFIG. 2 . As shown inFIG. 6 (a), in the porous membrane cartridge 1A of the second embodiment abead 16 a of which a section is chevron is provided across a whole perimeter in the vicinity of an outer perimeter of an openingedge 16 of thebarrel 10. Then an inner perimeter side of thebead 16 a is designed to be aflat portion 16 b for orthogonalizing an axial line of thebarrel 10. - In a case of assembling such the porous membrane cartridge 1A, disposing the porous membrane F at the
bottom portion 26 of thecap 20 and fitting thetop end 13 of thebarrel 10 in the fit-inportion 22 of thecap 20, sandwich the membrane F between the openingedge 16 and the sandwichingface 24. - Then if applying an ultrasound to the
barrel 10 by horn while pressing the openingedge 16 to the sandwichingface 24 in order to weld thecap 20 and thebarrel 10 by ultrasonic welder, thebead 16 a melts and penetrates the porous membrane F, and as shown inFIG. 6 (b), the sandwichingface 24 and thebead 16 a are welded. If further making the welding proceed, theflat portion 16 b crushes and sandwiches the porous membrane F between itself and the sandwichingface 24 as shown inFIG. 6 (c). - Also in the porous membrane cartridge 1A thus made, because the opening
edge 16 and the sandwichingface 24 are welded while the peripheral edge Fa of the porous membrane F melts, a liquid does not go around a side portion of the porous membrane F. In addition, because the porous membrane F is sandwiched between theflat portion 16 b and the sandwichingface 24 while crushed between them, a liquid does not enter the portion Fa of the membrane F. - Accordingly, also in the porous membrane cartridge 1A of the embodiment, a recovery efficiency of nucleic acids is better and mixing of impurities in a solution after a recovery is less.
- In addition, in a case of using the porous membrane cartridge 1A in filtration, mixing of impurities in a liquid after the filtration becomes less.
- Meanwhile, in a case of providing such a bead are available not only a mode of providing the bead at the opening
edge 16 of thebarrel 10 but also aporous membrane cartridge 1C of a mode where abead 24 b of which a section is chevron across a whole perimeter in the vicinity of the outer perimeter of the sandwichingface 24 without providing the bead at the openingedge 16 as shown inFIG. 7 . -
FIG. 8 is drawings showing aporous membrane cartridge 1D related to a third embodiment of the present invention and corresponding to the enlarged view of the part A inFIG. 2 . Theporous membrane cartridge 1D is a mode of welding thebarrel 10 and thecap 20 at a portion of an outer perimeter more than the sandwichingface 24. - In other words, as shown in
FIG. 8 (a), in thecap 20 is formed astep portion 27 for welding defined by: awall face portion 27 a perpendicularly rising up with respect to the sandwichingface 24 at outside of the sandwichingface 24; and aslant portion 27 b slantedly rising up toward the inner perimeter of the fit-inportion 22 from an upper end (namely, the rear end) inFIG. 8 (a) of thewall face portion 27 a. - On the other hand, at an opening
edge 17 of thebarrel 10 are formed aflat portion 17 a parallel with the sandwichingface 24 formed at an inner perimeter side; a wall face portion 17 b composed of a peripheral face for fitting in thewall face portion 27 a formed with continuing from an outer perimeter end of theflat portion 17 a; aslant portion 17 c formed with continuing from the top end inFIG. 8 (a) of thewall face portion 27 a and parallel with theslant portion 27 b; and aflat portion 17 d formed with continuing into theslant portion 17 c and extending toward the outer perimeter of thebarrel 10. - If such the
porous membrane cartridge 1D sandwiches the porous membrane F between the sandwichingface 24 and theflat portion 17 a, and thebarrel 10 and thecap 20 is applied to an ultrasonic welder, theflat portion 17 d abuts with theslant portion 27 b and the abutment portion is welded. Then the peripheral edge Fa of the porous membrane F is sandwiched between theflat portion 17 a and the sandwichingface 24 in a state of being crushed. - Thus only sandwiching and holding the porous membrane F and welding the
barrel 10 and thecap 20 at an outer portion more than the membrane F, it does not occur to damage the membrane F also in a case that it is especially delicate. - Furthermore, as a mode of fixing the
barrel 10 and thecap 20 at outside of the porous membrane F are cited modes shown inFIG. 9 . - For example, as shown in
FIG. 9 (a), it is also enabled to make themain body 12 of thebarrel 10 and a face of a step difference of an outer perimeter face of thetop end 13 ajoint portion 18 for welding, on the other hand to form a protrusion abutting with theportion 18 across a whole perimeter at an openingedge 28 at a rear end side of thecap 20, and to thus make aporous membrane cartridge 1E where thejoint portion 18 and the openingedge 28 are welded. - In addition, as shown in
FIG. 9 (b), form abead 19 composed of a gentle circular section along a peripheral direction across a whole perimeter at an outer perimeter of thetop end 13 of thebarrel 10, and on the other hand, in advance form a correspondingdepression groove 29 at a portion corresponding to thebead 19 on an inner perimeter face of the fit-inportion 22 of thecap 20. Then by an engagement between thebead 19 and thedepression groove 29, it is enabled to sandwich the porous membrane F between the openingedge 14 and the sandwichingface 24 in a state of crushing the outer perimeter edge of the membrane F. - Because such a
porous membrane cartridge 1F is enabled to be assembled by hand, it is convenient in small production. - Thus, although the embodiments of the present invention are described, the invention is not limited thereto, and it can be carried out with being appropriately varied.
- For example, the
barrel 10 and thecap 20 can also be made to adhere by any one of an adhesive and a UV curing resin, and the outer perimeter face of thetop end 13 of thebarrel 10 and the inner perimeter face of the fit-inportion 22 of thecap 20 can also be welded by laser. Meanwhile, in a case of laser welding, it is preferable to make the barrel 10 a material that can generate heat by absorbing laser or to blend a plasticizer in thebarrel 10. - Next will be described one example of the present invention.
- In the example, manufacturing a porous membrane cartridge of a shape in the first embodiment and changing a welding condition at that time, a test for determining whether welding was good or bad was performed.
- As a material of the
barrel 10 and thecap 20 was used polystyrene (manufactured by A&M Styrene Co., Ltd.). The outer diameter of the openingedge 14 of thebarrel 10 was made 7 mm, the width of theflat portion 14 a at outer perimeter side of openingedge 14 was made 0.05 mm, and as shown inFIG. 5 (a), the angle of thetaper portion 14 b was formed to be 10 degrees for theflat portion 14 a. - As the porous membrane F was used triacetylcellulose (Microfilter FM500 manufactured by Fuji Photo Film Co., Ltd.).
- Using an ultrasonic welder (40 KHz, 800 KW) manufactured by Branson Corp. as an ultrasonic welder, disposing the porous membrane F at the
bottom portion 26 of thecap 20 same as in the first embodiment, and fitting thetop end 13 of thebarrel 10 in thecap 20, thebarrel 10 was vibrated while pressed toward thecap 20 by a horn of the ultrasonic welder. Changing a pressing force and a vibration time at this time, a welding result under each condition was determined. - Table 1 shows the result.
TABLE 1 Pressing Force (N) 50 75 100 150 200 Vibration 0.03 X X X X X Time (sec) 0.05 X X ◯ ◯ ◯ 0.1 X X ◯ ◯ ◯ 0.15 X ◯ ◯ ◯ ◯ 0.2 Δ ◯ ◯ ◯ ◯ 0.25 Δ ◯ ◯ ◯ ◯ 0.3 Δ ◯ ◯ ◯ □ 0.35 Δ ◯ ◯ ◯ □ 0.4 Δ ◯ ◯ □ □ 0.45 Δ ◯ ◯ □ □ 0.5 Δ □ □ □ □ 0.6 Δ □ □ □ □
X Unable to be Welded
Δ Liquid Leakage
□ Perforation of Porous Membrane and Deformation of Molded Product
◯ Good
- As shown in Table 1 were changed the pressing force between 50 N and 200 N and the vibration time between 0.03 sec and 0.6 sec. In Table 1, the symbol x indicates that the
barrel 10 and thecap 20 could not be welded; the symbol Δ indicates that although thebarrel 10 and thecap 20 could be welded, the welding was insufficient and there was a liquid leakage from the welded portion; the symbol □ indicates that there was a defect that the porous membrane F was perforated or a formed product was deformed; and the symbol O indicates that the welding could be performed well without the defect. - As seen from Table 1, in a case of the pressing force of 50 N, the welding could not be performed or there was a liquid leakage, and any case of the vibration time was bad.
- In a case of the pressing force of 75 N, the welding could not be performed in a case of the vibration time not more than 0.1 sec and could be performed well in a case of the vibration time between 0.15 sec and 0.45 sec; in a case of the vibration time not less than 0.5 sec, a welding time was too long and there was the defect in a formed product.
- In a case of the pressing force of 100 N, the welding could not be performed in a case of the vibration time of 0.03 sec and could be performed well in a case of the vibration time between 0.05 sec and 0.45 sec; in a case of the vibration time not less than 0.5 sec, a welding time was too long and there was the defect in a formed product.
- In a case of the pressing force of 150 N, the welding could not be performed in a case of the vibration time of 0.03 sec and could be performed well in a case of the vibration time between 0.05 sec and 0.35 sec; in a case of the vibration time not less than 0.4 sec, a welding time was too long and there was the defect in a formed product.
- In a case of the pressing force of 200 N, the welding could not be performed in a case of the vibration time of 0.03 sec and could be performed well in a case of the vibration time between 0.05 sec and 0.25 sec; in a case of the vibration time not less than 0.3 sec, a welding time was too long and there was the defect in a formed product.
- Thus in a case of the pressing force between 75 N and 200 N, good welding was enabled if between a predetermined vibration time, and as a result, it was enabled to manufacture a preferable porous membrane cartridge where a liquid leakage does not occur.
Claims (9)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12/891,293 US20110072636A1 (en) | 2003-10-17 | 2010-09-27 | Method of assembling a porous membrane cartridge |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2003358256 | 2003-10-17 | ||
JP2003-358256 | 2003-10-17 | ||
PCT/JP2004/014156 WO2005037413A1 (en) | 2003-10-17 | 2004-09-28 | Porous film cartridge |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US12/891,293 Division US20110072636A1 (en) | 2003-10-17 | 2010-09-27 | Method of assembling a porous membrane cartridge |
Publications (1)
Publication Number | Publication Date |
---|---|
US20070017914A1 true US20070017914A1 (en) | 2007-01-25 |
Family
ID=34463287
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US10/575,366 Abandoned US20070017914A1 (en) | 2003-10-17 | 2004-09-28 | Porous film cartridge |
US12/891,293 Abandoned US20110072636A1 (en) | 2003-10-17 | 2010-09-27 | Method of assembling a porous membrane cartridge |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US12/891,293 Abandoned US20110072636A1 (en) | 2003-10-17 | 2010-09-27 | Method of assembling a porous membrane cartridge |
Country Status (4)
Country | Link |
---|---|
US (2) | US20070017914A1 (en) |
EP (1) | EP1683566A4 (en) |
JP (1) | JPWO2005037413A1 (en) |
WO (1) | WO2005037413A1 (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20120145704A1 (en) * | 2009-09-02 | 2012-06-14 | Nestec S.A. | One-piece packaging comprising a container and a closure |
CN105381642A (en) * | 2015-12-11 | 2016-03-09 | 浙江大学 | Rapid filtering apparatus used for low viscosity liquid |
US10913060B2 (en) * | 2011-11-10 | 2021-02-09 | Biofire Diagnostics, Llc | Loading vials |
US10928116B2 (en) * | 2019-02-27 | 2021-02-23 | Electrolux Home Products, Inc. | Modular water storage tank for a refrigerator |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4551174B2 (en) * | 2004-09-30 | 2010-09-22 | 富士フイルム株式会社 | Porous membrane cartridge and manufacturing method thereof |
JP2014030397A (en) * | 2012-08-03 | 2014-02-20 | Toppan Printing Co Ltd | Porous filter column, reagent cartridge, and nucleic acid refining kit |
WO2020175416A1 (en) | 2019-02-26 | 2020-09-03 | 富士フイルム株式会社 | Hydrophilic porous membrane and method for producing hydrophilic porous membrane |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3932153A (en) * | 1974-02-14 | 1976-01-13 | John Byrns | Nebulizer bacteria filter |
US4014653A (en) * | 1974-12-26 | 1977-03-29 | Denver Chemical Manufacturing Company | Micro-filter |
US4287065A (en) * | 1979-10-30 | 1981-09-01 | Burron Medical Inc. | Filter hub assembly |
US5096575A (en) * | 1988-02-12 | 1992-03-17 | Schleicher & Schuell Gmbh | Disposable filter unit |
US5688460A (en) * | 1994-04-26 | 1997-11-18 | Filtertek Inc. | Process for making hermetically sealed filter units |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS61803U (en) * | 1984-06-07 | 1986-01-07 | 科学技術庁放射線医学総合研究所長 | ultrafiltration device |
DE8435227U1 (en) * | 1984-12-01 | 1985-02-28 | B. Braun Melsungen Ag, 3508 Melsungen | FILTERS FOR BLOOD-CONDUCTING LINE SYSTEMS |
JPH03249926A (en) * | 1990-02-28 | 1991-11-07 | Ishikawa Seisakusho:Kk | Filter device |
JPH0595546U (en) * | 1992-06-03 | 1993-12-27 | 株式会社石川製作所 | Extracorporeal blood circuit drip chamber |
US5308483A (en) * | 1992-08-27 | 1994-05-03 | Gelman Sciences Inc. | Microporous filtration funnel assembly |
US5755269A (en) * | 1993-12-09 | 1998-05-26 | Ciba Corning Diagnostics Corp. | Fluid delivery system |
SE9400436D0 (en) * | 1994-02-10 | 1994-02-10 | Pharmacia Lkb Biotech | Ways to make filter wells |
DE4434800C2 (en) * | 1994-09-29 | 1996-09-05 | Porsche Ag | Filters for motor vehicles |
JPH09196911A (en) * | 1996-01-19 | 1997-07-31 | Fuji Photo Film Co Ltd | Blood filter unit |
JPH10277552A (en) * | 1997-04-08 | 1998-10-20 | Sanyo Electric Co Ltd | Diaphragm body of ionic water generating device |
US6391241B1 (en) * | 1997-06-06 | 2002-05-21 | Corning Incorporated | Method of manufacture for a multiwell plate and/or filter plate |
JP2002345465A (en) * | 2001-05-24 | 2002-12-03 | Fuji Photo Film Co Ltd | Unit for purifying nucleic acid and method for purifying the nucleic acid |
-
2004
- 2004-09-28 US US10/575,366 patent/US20070017914A1/en not_active Abandoned
- 2004-09-28 WO PCT/JP2004/014156 patent/WO2005037413A1/en active Application Filing
- 2004-09-28 JP JP2005514727A patent/JPWO2005037413A1/en active Pending
- 2004-09-28 EP EP04788223A patent/EP1683566A4/en not_active Withdrawn
-
2010
- 2010-09-27 US US12/891,293 patent/US20110072636A1/en not_active Abandoned
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3932153A (en) * | 1974-02-14 | 1976-01-13 | John Byrns | Nebulizer bacteria filter |
US4014653A (en) * | 1974-12-26 | 1977-03-29 | Denver Chemical Manufacturing Company | Micro-filter |
US4287065A (en) * | 1979-10-30 | 1981-09-01 | Burron Medical Inc. | Filter hub assembly |
US5096575A (en) * | 1988-02-12 | 1992-03-17 | Schleicher & Schuell Gmbh | Disposable filter unit |
US5688460A (en) * | 1994-04-26 | 1997-11-18 | Filtertek Inc. | Process for making hermetically sealed filter units |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20120145704A1 (en) * | 2009-09-02 | 2012-06-14 | Nestec S.A. | One-piece packaging comprising a container and a closure |
US8820550B2 (en) * | 2009-09-02 | 2014-09-02 | Nestec S.A. | One-piece packaging comprising a container and a closure |
US10913060B2 (en) * | 2011-11-10 | 2021-02-09 | Biofire Diagnostics, Llc | Loading vials |
CN105381642A (en) * | 2015-12-11 | 2016-03-09 | 浙江大学 | Rapid filtering apparatus used for low viscosity liquid |
US10928116B2 (en) * | 2019-02-27 | 2021-02-23 | Electrolux Home Products, Inc. | Modular water storage tank for a refrigerator |
Also Published As
Publication number | Publication date |
---|---|
EP1683566A4 (en) | 2008-09-24 |
EP1683566A1 (en) | 2006-07-26 |
JPWO2005037413A1 (en) | 2007-11-22 |
WO2005037413A1 (en) | 2005-04-28 |
US20110072636A1 (en) | 2011-03-31 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US20110072636A1 (en) | Method of assembling a porous membrane cartridge | |
JP5087096B2 (en) | Nucleic acid separation and purification cartridge | |
JP5019835B2 (en) | Filtration module | |
EP2086681A2 (en) | Small volume unitary molded filters and supports for adsorbent beds | |
EP1804972B1 (en) | Multiple cartridge, cartridge array frame | |
JP2007014959A5 (en) | ||
JP4406344B2 (en) | Porous membrane cartridge and manufacturing method thereof | |
WO2010131514A1 (en) | Microchip | |
JP2024056748A (en) | Apparatus and method of manufacture for membrane-based bind-and-elute chromatography - Patents.com | |
EP1807207B1 (en) | Multiple cartridge and cartridge array frame | |
JP4551174B2 (en) | Porous membrane cartridge and manufacturing method thereof | |
JP5251983B2 (en) | Microchip manufacturing method | |
JP4568904B2 (en) | Filtration cartridge manufacturing method and hot plate welding apparatus | |
JPH01135361A (en) | Small filter and its manufacture | |
JP2006102566A (en) | Production method for porous membrane cartridge packing article and porous membrane cartridge packing article | |
WO2010016399A1 (en) | Microchip, method for fabricating microchip and apparatus for fabricating microchip | |
JP3765680B2 (en) | Blood filter | |
JPWO2010016371A1 (en) | Microchip, microchip manufacturing method, and microchip manufacturing apparatus | |
KR20240073170A (en) | Device for bind and elute chromatography using membranes, and method of manufacture | |
JP2006055720A (en) | Separation membrane module and its production method, and airtight test method of separation membrane module |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AS | Assignment |
Owner name: FUJI PHOTO FILM CO., LTD., JAPAN Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:SHIGESADA, KEIJI;FUJIWARA, MORIO;REEL/FRAME:017798/0914 Effective date: 20060210 |
|
AS | Assignment |
Owner name: FUJIFILM CORPORATION, JAPAN Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:FUJIFILM HOLDINGS CORPORATION (FORMERLY FUJI PHOTO FILM CO., LTD.);REEL/FRAME:018904/0001 Effective date: 20070130 Owner name: FUJIFILM CORPORATION,JAPAN Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:FUJIFILM HOLDINGS CORPORATION (FORMERLY FUJI PHOTO FILM CO., LTD.);REEL/FRAME:018904/0001 Effective date: 20070130 |
|
STCB | Information on status: application discontinuation |
Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION |