TWM632402U

TWM632402U - PCR comprehensive diagnosis instrument

Info

Publication number: TWM632402U
Application number: TW111206374U
Authority: TW
Inventors: 陳韡崧; 陳鴻彬
Original assignee: 財桂生物股份有限公司
Priority date: 2022-06-16
Filing date: 2022-06-16
Publication date: 2022-09-21

Abstract

一種PCR綜合診斷儀，主要係包含一機體，以及設於該機體內之反應測試管載台及液體處理模組以及PCR檢測模組。其中，該反應測試管載台係供承載PCR試劑及檢體萃取液，且確保防止交叉污染；該液體處理模組係供為聚合酶連鎖反應測試管移動及蓋帽，以及運輸聚合酶連鎖反應測試管至PCR檢測模組；該PCR檢測模組係提供準確及大量的DNA / RNA增幅及檢測。藉此，本PCR綜合診斷儀可自動進行整個聚合酶連鎖反應程序，能夠節省時間減少人為出錯及提升實驗室效能，進而用於醫療實驗室從大量樣品中，進行病毒偵測、精準醫療或法醫取證等，提供萃取及分析樣品DNA或RNA。A PCR comprehensive diagnostic instrument mainly includes a body, a reaction test tube carrier, a liquid processing module and a PCR detection module set in the body. Among them, the reaction test tube stage is used for carrying PCR reagents and sample extracts, and to ensure the prevention of cross-contamination; the liquid handling module is used for the movement and cap of the PCR test tube, and for transporting the polymerase chain reaction test. Tube to PCR Detection Module; This PCR Detection Module provides accurate and high-volume DNA/RNA amplification and detection. In this way, the PCR comprehensive diagnostic instrument can automatically carry out the entire polymerase chain reaction process, which can save time, reduce human errors and improve laboratory efficiency, and then be used in medical laboratories for virus detection, precision medicine or forensics from a large number of samples Forensics, etc., provide extraction and analysis of sample DNA or RNA.

Description

PCR綜合診斷儀PCR comprehensive diagnostic instrument

本創作係有關一種PCR綜合診斷儀，尤指一種可自動進行整個聚合酶連鎖反應程序，節省時間減少人為出錯及提升實驗室效能，進而用於醫療實驗室從大量樣品中，進行病毒偵測、精準醫療或法醫取證等，提供萃取及分析樣品DNA或RNA之PCR綜合診斷儀。This creation relates to a comprehensive PCR diagnostic instrument, especially one that can automatically perform the entire polymerase chain reaction process, save time, reduce human errors and improve laboratory efficiency, and then be used in medical laboratories to detect viruses from a large number of samples, For precision medicine or forensic evidence collection, etc., we provide a comprehensive PCR diagnostic instrument for extracting and analyzing sample DNA or RNA.

按；隨著現代生醫技術的發展及臨床需求，許多診斷方法需要針對目標基因進行絕對定量。舉例而言，新冠病毒等感染的病毒量及療程監控，在現有技術中，為避免相互干擾，qPCR技術須將標準品與待測樣品放在不同的孔洞中，因此，須使用相當多的孔洞數，如此可能影響實驗流程進行以及測試成本。是以，針對具有多個反應孔的測試載具，在運用qPCR技術對核酸樣品進行絕對定量時，如何改善實驗順暢度並降低測試成本，誠為目前所需研究的重要課題。Press; With the development of modern biomedical technology and clinical needs, many diagnostic methods require absolute quantification of target genes. For example, in the monitoring of the amount of virus and the course of treatment for infections such as the new coronavirus, in the prior art, in order to avoid mutual interference, the qPCR technology must place the standard and the sample to be tested in different holes, so a considerable number of holes must be used. number, which may affect the experimental process and the cost of testing. Therefore, for a test vehicle with multiple reaction wells, when using qPCR technology to perform absolute quantification of nucleic acid samples, how to improve the smoothness of the experiment and reduce the test cost is an important topic that needs to be researched at present.

而傳統上巢式PCR的做法是先在一容器中放置初次PCR所需之反應溶液，在經過初次PCR熱循環反應後，或是取出初次PCR產物與複次PCR反應溶液混合後再進行複次PCR反應，或是直接將複次PCR反應溶液加入初次PCR反應溶液中混合後再進行複次PCR反應；反轉錄PCR的做法則是先在一容器中進行反轉錄反應，將mRNA或RNA以反轉錄酶轉換成CDNA，再將CDNA加入PCR反應溶液中進行PCR熱循環反應。不管是巢式PCR或是反轉錄PCR在過程中都有經過反應溶液轉換容器或添加轉移的動作，這些額外的動作往往就增加反應溶液被污染的可能性。The traditional method of nested PCR is to place the reaction solution required for the primary PCR in a container, and after the primary PCR thermal cycling reaction, or take out the primary PCR product and mix it with the repeated PCR reaction solution, and then repeat the reaction. PCR reaction, or directly add the multiple PCR reaction solution to the primary PCR reaction solution and mix and then perform the multiple PCR reaction; the method of reverse transcription PCR is to perform reverse transcription reaction in a container first, and mRNA or RNA is reversed. The transcriptase is converted into cDNA, and the cDNA is added to the PCR reaction solution for PCR thermal cycling reaction. Whether it is nested PCR or reverse transcription PCR, there are actions to change the container or add and transfer the reaction solution during the process, and these additional actions often increase the possibility of contamination of the reaction solution.

申請人有鑑於此，經不斷研究、實驗，遂萌生設計一種PCR綜合診斷儀，可自動進行整個聚合酶連鎖反應程序，進而節省時間減少人為出錯及提升實驗室效能。In view of this, the applicant, after continuous research and experimentation, came to design a comprehensive PCR diagnostic instrument, which can automatically perform the entire polymerase chain reaction process, thereby saving time, reducing human errors and improving laboratory efficiency.

本創作之主要目的，即在提供一種PCR綜合診斷儀，可自動進行整個聚合酶連鎖反應程序，節省時間減少人為出錯及提升實驗室效能，進而用於醫療實驗室從大量樣品中，進行病毒偵測、精準醫療或法醫取證等，提供萃取及分析樣品DNA或RNA。The main purpose of this creation is to provide a comprehensive PCR diagnostic instrument that can automatically perform the entire polymerase chain reaction process, save time, reduce human errors and improve laboratory efficiency, and then be used in medical laboratories to detect viruses from a large number of samples. For testing, precision medicine or forensic forensics, etc., provide extraction and analysis of sample DNA or RNA.

前述之PCR綜合診斷儀，係包含一機體，以及設於該機體內之反應測試管載台及液體處理模組以及PCR檢測模組。其中，該反應測試管載台係供承載PCR試劑及檢體萃取液，且確保防止交叉污染；該液體處理模組係供為聚合酶連鎖反應測試管移動及蓋帽，以及運輸聚合酶連鎖反應測試管至PCR檢測模組；該PCR檢測模組係提供準確及大量的DNA / RNA增幅及檢測。藉此，本PCR綜合診斷儀可自動進行整個聚合酶連鎖反應程序，能夠節省時間減少人為出錯及提升實驗室效能，進而用於醫療實驗室從大量樣品中，進行病毒偵測、精準醫療或法醫取證等，提供萃取及分析樣品DNA或RNA。The aforementioned comprehensive PCR diagnostic apparatus includes a body, a reaction test tube carrier, a liquid processing module, and a PCR detection module set in the body. Among them, the reaction test tube stage is used for carrying PCR reagents and sample extracts, and to ensure the prevention of cross-contamination; the liquid handling module is used for the movement and cap of the PCR test tube, and for transporting the polymerase chain reaction test. Tube to PCR Detection Module; This PCR Detection Module provides accurate and high-volume DNA/RNA amplification and detection. In this way, the PCR comprehensive diagnostic instrument can automatically carry out the entire polymerase chain reaction process, which can save time, reduce human errors and improve laboratory efficiency, and then be used in medical laboratories for virus detection, precision medicine or forensics from a large number of samples Forensics, etc., provide extraction and analysis of sample DNA or RNA.

前述之PCR綜合診斷儀，其中該反應測試管載台係包含兩滑軌及置於該兩滑軌之平台，該兩滑軌係設於該承架，該平台兩側係分別設有導槽，使導槽嵌置於該兩滑軌。該承架係設有一傳動件，該傳動件係以一傳動帶連接於平台，俾藉由該傳動件帶動平台之前、後位移，使平台由出入口伸出或縮回於機體內。In the above-mentioned comprehensive PCR diagnostic instrument, wherein the reaction test tube carrier comprises two slide rails and a platform placed on the two slide rails, the two slide rails are arranged on the support frame, and the two sides of the platform are respectively provided with guide grooves , so that the guide grooves are embedded in the two slide rails. The support frame is provided with a transmission element, and the transmission element is connected to the platform by a transmission belt, so that the transmission element drives the front and rear displacement of the platform, so that the platform can be extended or retracted into the machine body through the entrance and exit.

前述之PCR綜合診斷儀，其中該液體處理模組係包含一機座，以及設於該機座之套置部、活塞軸棒。藉由該套置部套置針管，並藉由活塞軸棒的上、下移動，以供將PCR試劑注入反應測試管。In the above-mentioned comprehensive PCR diagnostic instrument, the liquid processing module comprises a base, a sleeve part and a piston shaft rod disposed on the base. The needle tube is sheathed by the sheathing part, and the piston shaft rod is moved up and down to inject the PCR reagent into the reaction test tube.

前述之PCR綜合診斷儀，其中該液體處理模組係可縱向、橫向移動於不同之耗材卡匣，以供將PCR試劑注入反應測試管，並將針管予以退卸；以及藉由套置部套置一個上蓋，藉由驅動裝置驅動活塞軸棒向下移動，將上蓋蓋合於反應測試管之開口，以供移動至PCR檢測模組檢測，進而提升檢測之效率。In the aforementioned comprehensive PCR diagnostic instrument, wherein the liquid processing module can be moved vertically and horizontally in different consumable cassettes for injecting PCR reagents into the reaction test tube and withdrawing the needle tube; Put an upper cover, drive the piston shaft rod to move down by the driving device, and cover the upper cover to the opening of the reaction test tube for moving to the PCR detection module for detection, thereby improving the detection efficiency.

請同時參閱圖1，並配合圖2至圖7係為本創作之立體圖及結構圖，以及耗材卡匣盤與反應測試管立體圖、液體控制模組立體圖。如圖所示，本創作係設有一機體１，於機體１設有一觸控顯示幕１１，以及一出入口１２。該機體１內部係設有一承架１３，於該承架１３設有一反應測試管載台２及一液體處理模組３以及一PCR檢測模組４。其中，該反應測試管載台２係包含兩滑軌２１及置於該兩滑軌２１之平台２２，該兩滑軌２１係設於該承架１３，該平台２２兩側係分別設有導槽２２１，使導槽２２１嵌置於該兩滑軌２１。該承架１３係設有一傳動件２３，該傳動件２３係以一傳動帶２４連接於平台２２，俾藉由該傳動件２３及傳動帶２４帶動平台２２之前、後位移，使平台２２由出入口１２伸出，或縮回於機體１內。Please refer to FIG. 1 at the same time, in conjunction with FIG. 2 to FIG. 7 , which are the three-dimensional views and structural views of the present creation, as well as the three-dimensional views of the consumable cartridge tray and the reaction test tube, and the three-dimensional views of the liquid control module. As shown in the figure, the present creation system is provided with a body 1 , a touch display screen 11 and an entrance 12 are arranged in the body 1 . A support frame 13 is arranged inside the body 1 , and a reaction test tube carrier 2 , a liquid processing module 3 and a PCR detection module 4 are arranged on the support frame 13 . Wherein, the reaction test tube stage 2 includes two slide rails 21 and a platform 22 placed on the two slide rails 21 , the two slide rails 21 are arranged on the support frame 13 , and two sides of the platform 22 are respectively provided with guide rails 22 . The grooves 221 are formed so that the guide grooves 221 are embedded in the two slide rails 21 . The supporting frame 13 is provided with a transmission member 23, and the transmission member 23 is connected to the platform 22 by a transmission belt 24, so that the transmission member 23 and the transmission belt 24 can drive the front and rear displacement of the platform 22, so that the platform 22 extends from the entrance 12. out, or retracted into the body 1.

該平台２２係設有預先填充萃取液孔盤區２５，以供放置耗材卡匣盤２６，該耗材卡匣盤２６係供放入複數之反應測試管２７；該等反應測試管２７係供容置檢體萃取液；平台２２的後緣係設有至少可容置有針管３７、上蓋３８及PCR試劑之置件區２８。The platform 22 is provided with a pre-filled extraction liquid hole area 25 for placing a consumable cartridge tray 26 for placing a plurality of reaction test tubes 27; the reaction test tubes 27 are used for storage The sample extraction solution is placed; the rear edge of the platform 22 is provided with a placement area 28 that can accommodate at least the needle tube 37, the upper cover 38 and the PCR reagent.

該液體處理模組３，係包含一機座３１，以及設於該機座３１之套置部３２、活塞軸棒３３。其中，該機座３１係於上方適當位置設有一驅動裝置３４，並於下方適當位置設有一定位部３５；該套置部３２係穿套於該定位部３５，且突露於定位部３５底緣，該活塞軸棒３３係穿設於該定位部３５及套置部３２，其頂緣係設有一概呈塊狀之頂持件３６，由頂持件３６以桿體連接於驅動裝置３４，藉由該驅動裝置３４驅動該活塞軸棒３３沿該定位部３５上、下移動，以及帶動該頂持件３６呈相同方向之上、下移動。俾藉由該套置部３２於置件區２８套置針管３７，藉由活塞軸棒３３的上、下移動，以供由置件區２８抽取PCR試劑與檢體萃取液混合。The liquid processing module 3 includes a base 31 , a sleeve 32 and a piston shaft rod 33 arranged on the base 31 . Wherein, the base 31 is provided with a driving device 34 at a proper position above, and a positioning portion 35 is provided at a proper position below; The piston shaft rod 33 passes through the positioning portion 35 and the sleeve portion 32, and its top edge is provided with a block-shaped jacking member 36, which is connected to the driving device 34 by a rod body. , the piston shaft rod 33 is driven by the driving device 34 to move up and down along the positioning portion 35, and the jacking member 36 is driven to move up and down in the same direction. In order to set the needle tube 37 in the setting area 28 by the sleeve part 32, and move the piston shaft rod 33 up and down, the PCR reagent extracted from the setting area 28 can be mixed with the sample extraction liquid.

該機座３１係固定於一架體５，於該架體５設有橫向之滑軌５１，該機座３１係嵌置於該滑軌５１；該架體５係設有一傳動件５２，使傳動件５２以傳動帶５３連接於該機座３１，俾藉由該傳動件５２及傳動帶５３帶動該機座３１沿滑軌５１呈橫向之位移。該架體５的兩側底緣係分別設有導槽５４，使該等導槽５４嵌置設於承架１３所設之滑軌５５，並於該架體５設有另一傳動件５６，藉由該傳動件５６的傳動，使該架體５得以沿滑軌５５呈縱向之位移。藉此，液體處理模組３係可縱向、橫向移動於不同之耗材卡匣盤２６，以供將PCR試劑注入該等反應測試管２７，並將針管３７予以退卸；以及藉由套置部３２於置件區２８套置一個上蓋３８，藉由驅動裝置３１驅動活塞軸棒３３向下移動，將上蓋３８蓋合於反應測試管２７之開口，以供移動至PCR檢測模組４檢測，進而提升檢測之效率。The machine base 31 is fixed on the frame body 5, and the frame body 5 is provided with a lateral slide rail 51, and the machine base 31 is embedded in the slide rail 51; the frame body 5 is provided with a transmission member 52 to make The transmission member 52 is connected to the base 31 by a transmission belt 53 , so that the transmission member 52 and the transmission belt 53 can drive the base 31 to move laterally along the slide rail 51 . Two bottom edges of the frame body 5 are respectively provided with guide grooves 54 , so that the guide grooves 54 are embedded in the slide rails 55 provided on the support frame 13 , and the frame body 5 is provided with another transmission member 56 , through the transmission of the transmission member 56, the frame body 5 can be longitudinally displaced along the slide rail 55. Thereby, the liquid processing module 3 can be moved longitudinally and laterally in different consumables cassette trays 26, so as to inject PCR reagents into the reaction test tubes 27 and withdraw the needle tubes 37; 32. An upper cover 38 is sleeved on the part placement area 28, the piston shaft rod 33 is driven to move downward by the driving device 31, and the upper cover 38 is covered with the opening of the reaction test tube 27 for moving to the PCR detection module 4 for detection. Thereby improving the efficiency of detection.

該PCR檢測模組４，係設有可供反應測試管２７嵌置之孔盤區４１。The PCR detection module 4 is provided with a hole panel area 41 in which the reaction test tube 27 can be embedded.

藉由前述構件的組合，構成PCR綜合診斷儀。俾於使用時，藉由機體１之觸控顯示幕１１設定操作，使反應測試管載台２之平台２２由出入口１２伸出，於該平台２２放置耗材卡匣盤２６及反應測試管２７，以供放入檢體萃取液，於放置定位後該平台２２縮入機體１內。該液體處理模組３係可移動於不同之耗材卡匣盤２６與置件區２８之間，以供將PCR試劑注入反應測試管２７，並將針管３７予以退卸，再藉由套置部３２套置一個上蓋３８，藉由驅動裝置３１驅動活塞軸棒３３向下移動，將上蓋３８蓋合於反應測試管２７之開口，再將反應測試管２７移動至PCR檢測模組４檢測，並將檢測的結果顯示於觸控顯示幕１１，以提升檢測之效率。By combining the aforementioned components, a PCR comprehensive diagnostic apparatus is constructed. When in use, the platform 22 of the reaction test tube carrier 2 is protruded from the inlet and outlet 12 through the setting operation of the touch display screen 11 of the body 1, and the consumables cartridge tray 26 and the reaction test tube 27 are placed on the platform 22. The platform 22 is retracted into the body 1 after being placed and positioned. The liquid processing module 3 can be moved between different consumable cassette trays 26 and the setting area 28 for injecting PCR reagents into the reaction test tube 27, and withdrawing the needle tube 37, and then using the sleeve section 32 is sleeved with an upper cover 38, the piston shaft rod 33 is driven downward by the driving device 31, the upper cover 38 is covered with the opening of the reaction test tube 27, and then the reaction test tube 27 is moved to the PCR detection module 4 for detection, and The detection result is displayed on the touch display screen 11 to improve the detection efficiency.

請參閱圖8，係為本創作之液體控制模組動作狀態圖。敬請配合圖1及圖7，如圖所示，本創作於使用時，係由機體１設有一觸控顯示幕１１設定操作，於啟動後，該反應測試管載台２將會由出入口１２，以供於平台２２之預先填充萃取液孔盤區２５放置耗材卡匣盤２６，並於該耗材卡匣盤２６放入裝有檢體萃取液之反應測試管２７，於放置完成後該反應測試管載台２縮回機體１內。該液體處理模組３係可移動於不同之耗材卡匣盤２６，以供將PCR試劑注入反應測試管２７，並將針管３７予以退卸，再藉由套置部３２套置一個上蓋３８，藉由驅動裝置３１驅動活塞軸棒３３向下移動，將上蓋３８蓋合於反應測試管２７之開口，再將反應測試管２７移動至PCR檢測模組４檢測，並將檢測的結果顯示於觸控顯示幕１１，以提升檢測之效率。藉此，本PCR綜合診斷儀可自動進行整個聚合酶連鎖反應程序，能夠節省時間減少人為出錯及提升實驗室效能，進而用於醫療實驗室從大量樣品中，進行病毒偵測、精準醫療或法醫取證等，提供萃取及分析樣品DNA或RNA。Please refer to FIG. 8 , which is an action state diagram of the liquid control module of this creation. Please cooperate with Fig. 1 and Fig. 7. As shown in the figure, when the invention is in use, the body 1 is provided with a touch display screen 11 for setting operation. , so that the consumables cassette tray 26 is placed in the pre-filled extraction liquid hole panel 25 of the platform 22, and the reaction test tube 27 containing the sample extraction liquid is placed in the consumables cassette tray 26. After the placement is completed, the reaction The test tube carrier 2 is retracted into the body 1 . The liquid processing module 3 can be moved to different consumable cartridge trays 26 for injecting PCR reagents into the reaction test tube 27, and the needle tube 37 is withdrawn, and then an upper cover 38 is sheathed by the sheathing part 32, The piston shaft rod 33 is driven to move downward by the driving device 31, the upper cover 38 is covered with the opening of the reaction test tube 27, and then the reaction test tube 27 is moved to the PCR detection module 4 for detection, and the detection result is displayed on the touch screen. Control screen 11 to improve the efficiency of detection. In this way, the PCR comprehensive diagnostic instrument can automatically carry out the entire polymerase chain reaction process, which can save time, reduce human errors and improve laboratory efficiency, and then be used in medical laboratories for virus detection, precision medicine or forensics from a large number of samples Forensics, etc., provide extraction and analysis of sample DNA or RNA.

前述實施例，僅為說明本創作之較佳實施方式，而非限制本創作之範圍，凡經由些微修飾、變更，仍不失本創作之要義所在，亦不脫本創作之精神範疇。The foregoing examples are only to illustrate the preferred implementations of the present creation, rather than limiting the scope of the present creation. All modifications and changes are made without losing the essence of the present creation, nor deviating from the spirit of the present creation.

綜上所述，本創作以反應測試管載台及液體處理模組以及PCR檢測模組，構成PCR綜合診斷儀。可自動進行整個聚合酶連鎖反應程序，節省時間減少人為出錯及提升實驗室效能，進而用於醫療實驗室從大量樣品中，進行病毒偵測、精準醫療或法醫取證等，提供萃取及分析樣品DNA或RNA。為一實用之設計，誠屬一俱新穎性之創作，爰依法提出專利之申請，祈鈞局予以審查，早日賜准專利，至感德便。To sum up, this creation consists of a reaction test tube carrier, a liquid processing module, and a PCR detection module to form a PCR comprehensive diagnostic instrument. It can automatically carry out the entire polymerase chain reaction process, save time, reduce human errors and improve laboratory efficiency, and then be used in medical laboratories to perform virus detection, precision medicine or forensic evidence collection from a large number of samples, and provide extraction and analysis of sample DNA. or RNA. It is a practical design, and it is a creation of novelty. I should file a patent application in accordance with the law, and I pray that the bureau will review it and grant the patent as soon as possible.

1:機體 11:觸控顯示幕 12:出入口 13:承架 2:反應測試管載台 21:滑軌 22:平台 221:導槽 23:傳動件 24:傳動帶 25:預先填充萃取液孔盤區 26:耗材卡匣盤 27:反應測試管 3:液體處理模組 31:機座 32:套置部 33:活塞軸棒 34:驅動裝置 35:定位部 36:頂持件 37:針管 38:上蓋 4:PCR檢測模組 41:孔盤區 5:架體 51:滑軌 52:傳動件 53:傳動帶 54:導槽 55:滑軌 56:傳動件 1: Body 11: Touch Display 12: Entrance and exit 13: Bracket 2: Reaction test tube stage 21: Slide rail 22: Platform 221: Guide groove 23: Transmission parts 24: Transmission belt 25: Pre-fill the extract hole panel 26: Consumables cassette tray 27: Reaction Test Tube 3: Liquid Handling Module 31: base 32: Set part 33: Piston shaft rod 34: Drive 35: Positioning part 36: top holder 37: Needle 38: upper cover 4: PCR detection module 41: Hole Panel 5: Frame 51: Slide rail 52: Transmission parts 53: Transmission belt 54: Guide groove 55: Slide rail 56: Transmission parts

圖1係本創作之立體圖。圖2、圖3及圖4、圖5係本創作之結構圖。圖6係本創作之耗材卡匣盤與反應測試管立體圖。圖7係本創作之液體處理模組立體圖。圖8本創作之液體控制模組動作狀態圖。 Figure 1 is a perspective view of this creation. Figure 2, Figure 3, Figure 4, Figure 5 are the structural diagrams of this creation. Figure 6 is a perspective view of the consumable cartridge tray and reaction test tube of the present invention. Figure 7 is a perspective view of the liquid processing module of the present invention. Figure 8 is an action state diagram of the liquid control module of this creation.

13:承架 13: Bracket

2:反應測試管載台 2: Reaction test tube stage

22:平台 22: Platform

25:預先填充萃取液孔盤區 25: Pre-fill the extract hole panel

26:耗材卡匣盤 26: Consumables cassette tray

27:反應測試管 27: Reaction Test Tube

28:置件區 28: Placement area

3:液體處理模組 3: Liquid Handling Module

31:機座 31: base

32:套置部 32: Set part

33:活塞軸棒 33: Piston shaft rod

34:驅動裝置 34: Drive

35:定位部 35: Positioning part

36:頂持件 36: top holder

4:PCR檢測模組 4: PCR detection module

41:孔盤區 41: Hole Panel

5:架體 5: Frame

51:滑軌 51: Slide rail

52:傳動件 52: Transmission parts

53:傳動帶 53: Transmission belt

54:導槽 54: Guide groove

55:滑軌 55: Slide rail

56:傳動件 56: Transmission parts

Claims

一種PCR綜合診斷儀，包含：一機體，係設有一出入口，內部係設有一承架；一反應測試管載台，係設於該承架，其設有一平台，於該平台設有預先填充萃取液孔盤區，以供放置耗材卡匣盤；以及至少可容置有針管、上蓋及PCR試劑之置件區，該耗材卡匣盤係供放入複數之反應測試管，以供容置檢體萃取液；該平台係可由該機體之該出入口伸出或縮回於該機體內；一PCR檢測模組，係設於該承架；一液體處理模組，係設於該承架，其包含一機座，以及設於該機座之套置部、活塞軸棒，其中，該機座係於上方適當位置設有一驅動裝置，藉由該驅動裝置驅動該套置部套置該針管，以供抽取PCR試劑；該機座係設於一架體，可沿該架體呈橫向之位移；該架體係設於該承架，使該架體可沿該承架呈縱向之位移；藉此，該液體處理模組係可縱向、橫向移動於不同之該耗材卡匣盤，以供將PCR試劑注入該等反應測試管與該檢體萃取液混合，並套置該上蓋將該上蓋蓋合於該等反應測試管後，移動至該PCR檢測模組檢測。 A PCR comprehensive diagnostic instrument, comprising: a body, which is provided with an entrance and exit, and a support frame is provided inside; A reaction test tube stage is set on the support frame, and is provided with a platform, and the platform is provided with a pre-filled extraction liquid hole disk area for placing the consumable cartridge tray; and can accommodate at least the needle tube, the upper cover and the In the setting area of PCR reagents, the consumables cassette tray is used for placing multiple reaction test tubes for accommodating the sample extract; the platform can be extended or retracted in the body through the inlet and outlet of the body; a PCR detection module, which is arranged on the supporting frame; A liquid processing module is installed on the support frame, which includes a base, and a sleeve part and a piston shaft rod are arranged on the base, wherein a driving device is installed at an appropriate position above the base, so as to The sleeve part is driven by the drive device to sleeve the needle tube for extracting PCR reagents; the base is installed on a frame body and can be displaced laterally along the frame body; the frame system is installed on the support frame, so that The rack body can be longitudinally displaced along the support rack; thereby, the liquid processing module can be moved longitudinally and laterally on different consumable cartridge trays for injecting PCR reagents into the reaction test tubes and the test tubes. The body extracts are mixed, and the upper cover is sleeved over the reaction test tubes, and then moved to the PCR detection module for detection.

如請求項1所述之PCR綜合診斷儀，其中，該承架係設有兩滑軌，該反應測試管載台之該平台兩側係分別設有導槽，使該導槽嵌置於該兩滑軌該兩滑軌，沿該承架呈前、後之位移。The PCR comprehensive diagnostic apparatus according to claim 1, wherein the support frame is provided with two slide rails, and guide grooves are respectively provided on both sides of the platform of the reaction test tube carrier, so that the guide grooves are embedded in the The two slide rails are displaced forward and backward along the support frame.

如請求項1所述之PCR綜合診斷儀，其中，該承架係設有一傳動件，該傳動件係以一傳動帶連接於該平台，俾藉由該傳動件帶動平台之前、後位移，使該平台由該出入口伸出或縮回於該機體。The PCR comprehensive diagnostic apparatus according to claim 1, wherein the support frame is provided with a transmission element, and the transmission element is connected to the platform by a transmission belt, so that the transmission element drives the front and rear displacement of the platform to make the The platform extends or retracts from the body through the entrance and exit.

如請求項1所述之PCR綜合診斷儀，其中，該液體處理模組之該機座係固定於一架體，於該架體設有橫向之滑軌，該機座係嵌置於該滑軌；該架體係設有一傳動件，使該傳動件以傳動帶連接於該機座，俾藉由該傳動件帶動該機座沿該滑軌呈橫向之位移。The PCR comprehensive diagnostic instrument according to claim 1, wherein the base of the liquid processing module is fixed to a frame body, a lateral slide rail is arranged on the frame body, and the base is embedded in the slide The frame system is provided with a transmission member, so that the transmission member is connected to the machine base with a transmission belt, so that the transmission piece drives the machine base to move laterally along the slide rail.

如請求項4所述之PCR綜合診斷儀，其中，該液體處理模組之該架體的兩側底緣係分別設有導槽，使該等導槽嵌置設於承架所設之滑軌，並於該架體設有一傳動件，藉由該傳動件的傳動，使該架體得以沿該滑軌呈縱向之位移。The PCR comprehensive diagnostic apparatus according to claim 4, wherein the bottom edges of the two sides of the frame body of the liquid processing module are respectively provided with guide grooves, so that the guide grooves are embedded in the slides provided by the support frame. The frame body is provided with a transmission member, and the frame body can be longitudinally displaced along the slide rail by the transmission of the transmission member.