TWM485907U - Magnetic bar structure of magnetic bead nucleic acid extracting detection system - Google Patents
Magnetic bar structure of magnetic bead nucleic acid extracting detection system Download PDFInfo
- Publication number
- TWM485907U TWM485907U TW103208224U TW103208224U TWM485907U TW M485907 U TWM485907 U TW M485907U TW 103208224 U TW103208224 U TW 103208224U TW 103208224 U TW103208224 U TW 103208224U TW M485907 U TWM485907 U TW M485907U
- Authority
- TW
- Taiwan
- Prior art keywords
- magnetic
- nucleic acid
- rod
- test tube
- detection system
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title claims description 55
- 102000039446 nucleic acids Human genes 0.000 title claims description 55
- 108020004707 nucleic acids Proteins 0.000 title claims description 55
- 239000011324 bead Substances 0.000 title description 35
- 238000001514 detection method Methods 0.000 title description 14
- 238000007886 magnetic bead extraction Methods 0.000 claims description 13
- 239000007787 solid Substances 0.000 claims description 3
- 239000000872 buffer Substances 0.000 description 15
- 239000000243 solution Substances 0.000 description 15
- 238000000034 method Methods 0.000 description 11
- 239000000284 extract Substances 0.000 description 10
- 238000000605 extraction Methods 0.000 description 9
- 239000000203 mixture Substances 0.000 description 9
- 239000011259 mixed solution Substances 0.000 description 7
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 239000012535 impurity Substances 0.000 description 5
- 239000006166 lysate Substances 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 4
- 230000009089 cytolysis Effects 0.000 description 3
- 239000003292 glue Substances 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 239000012149 elution buffer Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000011536 extraction buffer Substances 0.000 description 2
- 238000009413 insulation Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 230000000149 penetrating effect Effects 0.000 description 2
- 241001391944 Commicarpus scandens Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 239000000696 magnetic material Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
本創作與磁珠萃取核酸之檢測系統有關，尤指一種磁珠萃取核酸檢測系統之磁棒結構。The present invention relates to a magnetic bead extraction nucleic acid detection system, and more particularly to a magnetic bead structure of a magnetic bead extraction nucleic acid detection system.
核酸分析是現今生物醫學研究與臨床診斷不可或缺的方法，因此，核酸的分離與萃取相關技術發展快速並已臻成熟。而如何結合相關技術更有效率地自生物採樣檢體或臨床檢體分離並萃取出高純度的核酸，已然成為本領域的發展目標。例如，利用帶有特殊官能基的微粒（以下稱作「磁珠」）以特異性之方式吸附檢體混合液中的核酸後，搭配使用磁性控制裝置（例如磁鐵）提供磁力或磁場，用以吸引並帶動前述磁珠，以利進行反應、沖洗及提取等各階段萃取核酸流程，此類萃取核酸的方法常稱為「磁珠式核酸萃取方法」。Nucleic acid analysis is an indispensable method for biomedical research and clinical diagnosis. Therefore, the technology of separation and extraction of nucleic acids has developed rapidly and matured. How to combine and extract high-purity nucleic acids from biological sampling samples or clinical samples more efficiently combined with related technologies has become a development goal in the field. For example, a particle having a specific functional group (hereinafter referred to as "magnetic bead") is used to specifically adsorb a nucleic acid in a sample mixture, and a magnetic control device (for example, a magnet) is used to supply a magnetic force or a magnetic field. The magnetic beads are attracted and driven to facilitate the process of extracting nucleic acids in various stages such as reaction, washing and extraction. Such a method for extracting nucleic acids is often referred to as a "magnetic bead nucleic acid extraction method".
一般習知磁珠式核酸萃取方法的操作過程與原理如第６圖所示，簡述如下：將含有已完成裂解程序之檢體５１與裂解液５２混合物之混合液５７及定量的磁珠５３加入試管５８中反應，此時檢體５１中的核酸已釋出至混合液５７中，使磁珠５３得以吸附混合液５７中的核酸，並以磁鐵５９吸住附有核酸之磁珠５３後，將試管５８內的溶液吸出；接著，將第一緩衝液５４加入試管５８中浸潤並沖洗吸住吸附有核酸之磁珠５３上之雜質後，並以磁鐵５９吸住吸附有核酸之磁珠５３後，將試管５８內的溶液吸出；繼續將第二緩衝液５５加入試管５８中浸潤並進一步沖洗吸住吸附有核酸之磁珠５３上之雜質，並以磁鐵５９吸住吸附有核酸之磁珠５３後，將試管５８內的溶液吸出。然而，前述以緩衝液沖洗吸附有核酸之磁珠５３之步驟可視使用者需求之清洗程度彈性增加或減為單一次沖洗步驟。最後，將沖提緩衝液５６加入試管５８中，以沖提吸附有核酸之磁珠５３上之核酸，即可得到核酸萃取物，而此時核酸萃取物已溶解於沖提緩衝液５６中；最後，將以磁鐵５９吸住磁珠５３，將試管５８內的溶液吸出，此溶液即為含有核酸萃取物之混合溶液５０。The operation process and principle of the conventional magnetic bead nucleic acid extraction method are as shown in FIG. 6 and are briefly described as follows: a mixture 57 containing a mixture of the sample 51 and the lysate 52 having completed the lysis procedure and a quantitative magnetic bead 53 The reaction in the test tube 58 is carried out, at which time the nucleic acid in the sample 51 has been released into the mixed solution 57, so that the magnetic beads 53 can adsorb the nucleic acid in the mixed solution 57, and the magnetic beads 53 are attracted by the magnet 59. The solution in the test tube 58 is aspirated; then, the first buffer 54 is added to the test tube 58 to infiltrate and rinse the impurities on the magnetic beads 53 adsorbed with the nucleic acid, and the magnetic beads are adsorbed by the magnet 59. After 53, the solution in the test tube 58 is aspirated; the second buffer 55 is continuously added to the test tube 58 to be infiltrated and further washed to absorb the impurities on the magnetic beads 53 to which the nucleic acid is adsorbed, and the magnet 59 is attracted to the magnetic field to which the nucleic acid is adsorbed. After the beads 53, the solution in the test tube 58 is aspirated. However, the aforementioned step of rinsing the nucleic acid-adsorbing magnetic beads 53 with a buffer may be elastically increased or reduced to a single-rinsing step depending on the degree of cleaning desired by the user. Finally, the buffering buffer 56 is added to the test tube 58 to extract the nucleic acid adsorbed on the magnetic beads 53 of the nucleic acid, thereby obtaining the nucleic acid extract, and at this time, the nucleic acid extract has been dissolved in the elution buffer 56; Finally, the magnetic beads 53 are sucked by the magnet 59, and the solution in the test tube 58 is aspirated. This solution is a mixed solution 50 containing the nucleic acid extract.
由於上述磁珠式核酸萃取方法的純化原理，採用不同性質的磁珠５３並配合特定的磁場條件，可使磁珠５３產生聚集或分散等現象，因此可排除另一類常用於萃取核酸之「離心式核酸萃取方法」所需要的繁複人工操作流程，故磁珠式核酸萃取方法非常適合自動化，於短時間內處理大量檢體，進而達到高通量（high-throughput）核酸萃取之目標。由於以自動化操作系統完全取代人工操作流程減少人力需求，除了可達成提高效率提及經濟效益的優點，還可減少人工操作所帶來的人為誤差、樣品交叉污染、及高危險感染性檢體等問題，例如使用了錯誤試劑或錯誤判讀數值、樣品潑灑及混淆樣品與人之間的正確關聯。是以，磁珠式核酸萃取方法之自動化為往後必然的發展方向。Due to the purification principle of the magnetic bead nucleic acid extraction method described above, the use of magnetic beads 53 of different properties and specific magnetic field conditions can cause the magnetic beads 53 to aggregate or disperse, thereby eliminating another type of "centrifugation" commonly used for nucleic acid extraction. The nucleic acid extraction method is very suitable for automation, and a large number of samples are processed in a short time, thereby achieving the goal of high-throughput nucleic acid extraction. Since the automated operating system completely replaces the manual operation process and reduces the manpower requirement, in addition to the advantages of improving efficiency and mentioning economic benefits, it can also reduce human error caused by manual operation, sample cross-contamination, and high-risk infectious samples. Problems such as the use of incorrect reagents or false positive readings, sample spills, and confusion between the sample and the person. Therefore, the automation of the magnetic bead nucleic acid extraction method is an inevitable development direction in the future.
將前述萃取核酸的流程步驟自動化之機械如第７圖所示，其包括有一槽體６，槽內經區隔形成有多個試管空間６１，而該槽體６上方設有可升降之磁吸結構７，其中該磁吸結構７對應各試管空間６１的位置分別設有一磁棒７１及一隔絕管７２。而此習用之自動機械的使用步驟如第８圖所示，簡述如下：將含有已完成裂解程序之檢體８１與裂解液８２混合物之混合液８７及定量的磁珠８３加入各試管空間６１中反應，使磁珠８３得以吸附混合液８７中的核酸，再將磁棒７１伸入隔絕管７２中，並將兩者一起降下而伸入試管空間６１內，藉由磁棒７１之磁力吸附磁珠８３後升起磁棒７１及隔絕管７２，且將試管空間６１內的溶液吸出；接著，將第一緩衝液８４加入試管空間６１中浸潤並沖洗吸住吸附有核酸之磁珠８３上之雜質後，再將磁棒７１伸入隔絕管７２中，並將兩者一起降下而伸入試管空間６１內，藉由磁棒７１之磁力吸附磁珠８３後升起磁棒７１及隔絕管７２，且將試管空間６１內的溶液吸出；繼續將第二緩衝液８５加入試管空間６１中浸潤並進一步沖洗吸住吸附有核酸之磁珠８３上之雜質，再將磁棒７１伸入隔絕管７２中，並將兩者一起降下而伸入試管空間６１內，藉由磁棒７１之磁力吸附磁珠８３後升起磁棒７１及隔絕管７２，且將試管空間６１內的溶液吸出；然後將沖提緩衝液８６加入試管空間６１中，以沖提吸附有核酸之磁珠８３上之核酸，即可得到核酸萃取物，而此時核酸萃取物已溶解於沖提緩衝液８６中；最後，將磁棒７１伸入隔絕管７２中，並將兩者一起降下而伸入試管空間６１內，藉由磁棒７１之磁力吸附磁珠８３後升起磁棒７１及隔絕管７２，並將試管空間６１內的溶液吸出，此溶液即為含有核酸萃取物之混合溶液８０。The machine for automating the above-mentioned process for extracting nucleic acid is as shown in Fig. 7, which comprises a tank body 6 in which a plurality of test tube spaces 61 are formed, and a magnetic body capable of lifting and lowering is provided above the tank body 6. The structure 7 is characterized in that the magnetic structure 7 is provided with a magnetic bar 71 and an insulating tube 72 respectively corresponding to the positions of the test tube spaces 61. The procedure for the use of the conventional automatic machine is as shown in Fig. 8, and is briefly described as follows: a mixture 87 of the mixture of the sample 81 and the lysate 82 containing the completed lysis procedure and a quantitative magnetic bead 83 are added to the respective test tube spaces 61. In the middle reaction, the magnetic beads 83 are allowed to adsorb the nucleic acid in the mixed liquid 87, and then the magnetic rod 71 is inserted into the insulating tube 72, and the two are lowered together to extend into the test tube space 61, and magnetically attracted by the magnetic rod 71. After the magnetic beads 83, the magnetic rod 71 and the insulating tube 72 are raised, and the solution in the test tube space 61 is aspirated; then, the first buffer 84 is added to the test tube space 61 to be infiltrated and rinsed to attract the magnetic beads 83 to which the nucleic acid is adsorbed. After the impurities, the magnetic rod 71 is inserted into the insulating tube 72, and the two are lowered together to extend into the test tube space 61. The magnetic rods 83 are attracted by the magnetic force of the magnetic rod 71, and the magnetic rod 71 and the insulating tube are raised. 72, and the solution in the test tube space 61 is aspirated; the second buffer 85 is continuously added to the test tube space 61 to be infiltrated and further washed to absorb the magnetic beads adsorbed with the nucleic acid 8 The magnetic material is inserted into the insulating tube 72, and the two are lowered together to extend into the test tube space 61. The magnetic rods 83 are attracted by the magnetic force of the magnetic rod 71, and the magnetic rod 71 and the insulating tube are raised. 72, and the solution in the test tube space 61 is aspirated; then the extraction buffer 86 is added to the test tube space 61 to extract the nucleic acid adsorbed on the magnetic beads 83 of the nucleic acid, thereby obtaining the nucleic acid extract, and at this time, the nucleic acid The extract has been dissolved in the flushing buffer 86; finally, the magnetic rod 71 is inserted into the insulating tube 72, and the two are lowered together into the test tube space 61, and the magnetic beads are adsorbed by the magnetic force of the magnetic rod 71. Thereafter, the magnetic bar 71 and the insulating tube 72 are raised, and the solution in the test tube space 61 is aspirated. This solution is a mixed solution 80 containing the nucleic acid extract.
上述萃取核酸的步驟中，如第９圖所示，為避免磁棒７１直接接觸檢體，故必須在外套設該隔絕管７２。然而若磁棒７１稍有歪斜地伸入該隔絕管７２，即有碰觸隔絕管７２之管壁的風險。而磁棒７１之結構如第１０圖所示，其以一桿體７３與一磁塊７４組成，其中該桿體７３之底端凹設有一固定孔７３１，而該磁塊７４之外徑與該桿體７３相等，且其頂端凸出一固定柱７４１，該磁塊７４以其固定柱７４１插設於該桿體７３之固定孔７３１中，並利用缺氧膠輔助黏著，藉以將該磁塊７４固定於該桿體７３之底端。承上述，由於磁塊７４的質地較脆，稍有碰撞就很容易斷裂損壞，是其缺點。最直接的改善方式為將磁棒７１的外徑細小化，以減少碰撞發生的機會，然而磁棒７１外徑細小化意謂桿體７３之固定孔７３１及磁塊７４之固定柱７４１皆隨之縮細，二者材料上之結構強度皆不足以支持磁棒的小型化，因此必須尋求其他的磁棒組合方式。In the step of extracting the nucleic acid, as shown in Fig. 9, in order to prevent the magnetic bar 71 from directly contacting the sample, the insulating tube 72 must be provided on the outer casing. However, if the magnetic bar 71 projects slightly obliquely into the insulating tube 72, there is a risk of touching the wall of the insulating tube 72. The structure of the magnetic bar 71 is as shown in FIG. 10, which is composed of a rod 73 and a magnetic block 74. The bottom end of the rod 73 is recessed with a fixing hole 731, and the outer diameter of the magnetic block 74 is The rod body 73 is equal, and a fixing post 741 is protruded from the top end thereof. The magnetic block 74 is inserted into the fixing hole 731 of the rod body 73 with its fixing post 741, and is adhered by an anoxic glue, thereby magnetically A block 74 is fixed to the bottom end of the rod 73. In view of the above, since the texture of the magnetic block 74 is relatively brittle, it is easy to break and damage with a slight collision, which is a disadvantage. The most straightforward improvement is to reduce the outer diameter of the magnetic rod 71 to reduce the chance of collision. However, the outer diameter of the magnetic rod 71 is small, meaning that the fixing hole 731 of the rod 73 and the fixing column 741 of the magnetic block 74 follow. The shrinkage, the structural strength of the two materials is not enough to support the miniaturization of the magnetic bar, so other magnetic bar combination must be sought.
有鑑於此，故如何改進上述問題，即為本創作所欲解決之首要課題。In view of this, how to improve the above problems is the primary issue that the creative office wants to solve.
本創作目的之一在於提供一種磁珠萃取核酸檢測系統之磁棒結構，其將磁塊設於桿體內部，藉由桿體保護磁塊，避免受碰撞而損壞。One of the aims of the present invention is to provide a magnetic rod structure of a magnetic bead extraction nucleic acid detecting system, which is provided with a magnetic block inside the rod body, and the magnetic block is protected by the rod body to avoid damage by collision.
本創作目的之二在於藉由上述結構的配置方式，令磁棒之外徑可達到細小化的目的。The second purpose of the present invention is that the outer diameter of the magnetic bar can be miniaturized by the configuration of the above structure.
為達前述目的，本創作提供一種磁珠萃取核酸檢測系統之磁棒結構，其包括有一直棒，該直棒之其中至少一端凹設有一內孔，而該內孔中固設有一呈圓柱狀之磁塊。In order to achieve the foregoing objective, the present invention provides a magnetic rod structure of a magnetic bead extraction nucleic acid detecting system, comprising a straight rod, wherein at least one end of the straight rod is concavely provided with an inner hole, and the inner hole is fixed in a cylindrical shape Magnetic block.
較佳地，該內孔貫通於該直棒之兩端。Preferably, the inner hole penetrates through both ends of the straight rod.
較佳地，其中，該直棒為實心體，且該內孔設於該直棒之其中一端。Preferably, the straight rod is a solid body, and the inner hole is disposed at one end of the straight rod.
較佳地，該直棒之直徑為2公厘至3.7公厘。Preferably, the straight rod has a diameter of from 2 mm to 3.7 mm.
而本創作之上述目的與優點，不難從下述所選用實施例之詳細說明與附圖中獲得深入了解。The above objects and advantages of the present invention are not to be understood in detail from the detailed description and the accompanying drawings.
１‧‧‧試劑槽
１１‧‧‧隔板1‧‧‧Reagent tank 11‧‧‧ partition
１２‧‧‧試管空間
２‧‧‧磁吸結構12‧‧‧Test tube space 2‧‧ magnetic structure
３‧‧‧磁棒
３１Ａ、３１Ｂ‧‧‧直棒3‧‧‧Magnetic rod 31A, 31B‧‧‧ straight rod
３２Ａ、３２Ｂ‧‧‧內孔
３３‧‧‧磁塊32A, 32B‧‧‧ hole 33‧‧‧ magnetic block
４‧‧‧隔絕管4‧‧‧Isolation tube
５０‧‧‧混合溶液
５１‧‧‧檢體50‧‧‧ mixed solution 51‧‧‧ specimen
５２‧‧‧裂解液
５３‧‧‧磁珠52‧‧‧ lysate 53‧‧‧ magnetic beads
５４‧‧‧第一緩衝液
５５‧‧‧第二緩衝液54‧‧‧First buffer 55‧‧‧Second buffer
５６‧‧‧沖提緩衝液
５７‧‧‧混合液56‧‧‧Accumulation buffer 57‧‧‧ mixture
５８‧‧‧試管
５９‧‧‧磁鐵58‧‧‧Test tube 59‧‧‧ magnet
６‧‧‧槽體
６１‧‧‧試管空間6‧‧‧Slot 61‧‧‧Test tube space
７‧‧‧磁吸結構
７１‧‧‧磁棒7‧‧‧Magnetic structure 71‧‧‧Magnetic rod
７２‧‧‧隔絕管
８０‧‧‧混合溶液72‧‧‧Insulation tube 80‧‧‧ mixed solution
８１‧‧‧檢體
８２‧‧‧裂解液81‧‧‧ specimen 82‧‧ ‧ lysate
８３‧‧‧磁珠
８４‧‧‧第一緩衝液83‧‧‧Magnetic beads 84‧‧‧ first buffer
８５‧‧‧第二緩衝液
８６‧‧‧沖提緩衝液85‧‧‧Second buffer 86‧‧‧ rinse buffer
第１圖為應用本創作之檢測系統的立體示意圖Figure 1 is a perspective view of a detection system using the present creation
第２圖為應用本創作之檢測系統的使用狀態立體示意圖Figure 2 is a perspective view showing the state of use of the detection system to which the present application is applied.
第３圖為本創作第一實施例之剖面示意圖Figure 3 is a schematic cross-sectional view showing the first embodiment of the creation
第４圖為本創作第一實施例之使用狀態剖面示意圖Figure 4 is a schematic cross-sectional view showing the state of use of the first embodiment of the present invention.
第５圖為本創作第二實施例之剖面示意圖Figure 5 is a schematic cross-sectional view showing the second embodiment of the creation
第６圖為習知之磁珠萃取核酸檢測方法的步驟示意圖Figure 6 is a schematic diagram showing the steps of a conventional magnetic bead extraction nucleic acid detection method.
第７圖為習知之磁珠萃取核酸檢測系統的立體示意圖Figure 7 is a schematic perspective view of a conventional magnetic bead extraction nucleic acid detection system
第８圖為使用第７圖所示之檢測系統的檢測方法步驟示意圖Figure 8 is a schematic diagram showing the steps of the detection method using the detection system shown in Figure 7
第９圖為習知之磁珠萃取核酸檢測系統的使用狀態剖面示意圖Figure 9 is a schematic cross-sectional view showing the state of use of a conventional magnetic bead extraction nucleic acid detection system.
第１０圖為習知之磁珠萃取核酸檢測系統之磁棒結構的剖面示意圖Figure 10 is a schematic cross-sectional view showing the structure of a magnetic rod of a magnetic bead extraction nucleic acid detection system of the prior art.
請參閱第１、２圖，所示者為應用本創作之磁珠萃取核酸檢測系統，其包括有一試劑槽１，該試劑槽１內以多數隔板１１區隔形成有多數個呈格狀之試管空間１２，用以盛裝檢體；而該試劑槽１上方設有一可升降之磁吸結構２，其包括有多數磁棒３及多數隔絕管４，其中各磁棒３分別與一隔絕管４及一試管空間１２對應，俾供使用該檢測系統時，進行如下所述之步驟：將含有已完成裂解程序之檢體與裂解液混合物之混合液及定量的磁珠加入各試管空間１２中反應，使磁珠得以吸附混合液中的核酸，再將磁棒３伸入隔絕管４中，並將兩者一起降下而伸入試管空間１２內，藉由磁棒３之磁力吸附磁珠後升起磁棒３及隔絕管４，且將試管空間１２內的溶液吸出；接著，將第一緩衝液加入試管空間１２中浸潤並沖洗吸住吸附有核酸之磁珠上之雜質後，再將磁棒３伸入隔絕管４中，並將兩者一起降下而伸入試管空間１２內，藉由磁棒３之磁力吸附磁珠後升起磁棒３及隔絕管４，且將試管空間１２內的溶液吸出；繼續將第二緩衝液加入試管空間１２中浸潤並進一步沖洗吸住吸附有核酸之磁珠上之雜質，再將磁棒３伸入隔絕管４中，並將兩者一起降下而伸入試管空間１２內，藉由磁棒３之磁力吸附磁珠後升起磁棒３及隔絕管４，且將試管空間１２內的溶液吸出；然後將沖提緩衝液加入試管空間１２中，以沖提吸附有核酸之磁珠上之核酸，即可得到核酸萃取物，而此時核酸萃取物已溶解於沖提緩衝液中；最後，將磁棒３伸入隔絕管４中，並將兩者一起降下而伸入試管空間１２內，藉由磁棒３之磁力吸附磁珠後升起磁棒３及隔絕管４，並將試管空間１２內的溶液吸出，此溶液即為含有核酸萃取物之混合溶液。Please refer to Figures 1 and 2 for the magnetic bead extraction nucleic acid detection system of the present application, which comprises a reagent tank 1 in which a plurality of partitions 11 are partitioned to form a plurality of lattices. The test tube space 12 is used for accommodating the sample; and the reagent tank 1 is provided with a lifting and lowering magnetic structure 2, which comprises a plurality of magnetic rods 3 and a plurality of insulating tubes 4, wherein each of the magnetic rods 3 and an insulating tube 4 respectively Corresponding to a test tube space 12, when the detection system is used, the following steps are performed: a mixture of the sample containing the completed lysis procedure and the lysate mixture and the quantitative magnetic beads are added to each test tube space 12 for reaction. The magnetic beads are allowed to adsorb the nucleic acid in the mixed solution, and then the magnetic rod 3 is inserted into the insulating tube 4, and the two are lowered together to extend into the test tube space 12, and the magnetic beads are attracted by the magnetic force of the magnetic rod 3. Starting the magnetic rod 3 and the insulating tube 4, and sucking out the solution in the test tube space 12; then, adding the first buffer to the test tube space 12 to infiltrate and rinsing the impurities on the magnetic beads adsorbed with the nucleic acid, The magnetic rod 3 is inserted into the insulating tube 4, and the two are lowered together to extend into the test tube space 12, and the magnetic rod 3 and the insulating tube 4 are raised by the magnetic force of the magnetic rod 3, and the test tube space is raised. The solution in 12 is aspirated; the second buffer is further added to the test tube space 12 to be infiltrated and further washed to absorb the impurities on the magnetic beads adsorbed with the nucleic acid, and then the magnetic rod 3 is inserted into the insulating tube 4, and the two are together Lowering and extending into the test tube space 12, the magnetic rod 3 and the isolation tube 4 are raised by the magnetic force of the magnetic rod 3, and the solution in the test tube space 12 is sucked out; then the extraction buffer is added to the test tube space 12 The nucleic acid extract is obtained by extracting the nucleic acid adsorbed on the magnetic beads of the nucleic acid, and at this time, the nucleic acid extract is dissolved in the elution buffer; finally, the magnetic rod 3 is inserted into the insulating tube 4, The two are lowered together and inserted into the test tube space 12, and the magnetic rod 3 and the insulating tube 4 are lifted by the magnetic force of the magnetic rod 3, and the solution in the test tube space 12 is sucked out, and the solution is contained. Mix of nucleic acid extracts Liquid.
上述檢測系統所應用之本創作磁棒結構如第３圖所示者，其為本創作之第一實施例。此磁棒３ 包括有一直棒３１Ａ，該直棒３１Ａ之其中至少一端凹設有一內孔３２Ａ，而該內孔３２Ａ中固設有一呈圓柱狀之磁塊３３；其中於本實施例中，該內孔３２Ａ乃貫通於該直棒３１Ａ之兩端，令該直棒３１Ａ形成為一管狀結構，而於該直棒３１Ａ之一端以缺氧膠將該磁塊３３固定於該內孔３２Ａ中。藉此，該磁塊３３完全藏於該直棒３１Ａ中而不顯露於直棒３１Ａ外，藉以保護質地較脆之磁塊３３不受碰撞，若不慎遭受碰撞，其衝擊力量亦由該直棒３１Ａ承受，以保護磁塊３３不受損壞。另一方面，雖然該磁塊３３藏於該直棒３１Ａ之內部，惟其磁力仍可發揮，可穿透該直棒３１Ａ而吸附磁珠。The structure of the present invention as applied to the above-described detection system is as shown in Fig. 3, which is the first embodiment of the creation. The magnetic bar 3 includes a straight bar 31A, and at least one end of the straight bar 31A is recessed with an inner hole 32A, and the inner hole 32A is fixed with a cylindrical magnetic block 33; wherein in the embodiment, the magnetic bar 33 The inner hole 32A is penetrated through the two ends of the straight rod 31A, so that the straight rod 31A is formed into a tubular structure, and the magnetic block 33 is fixed in the inner hole 32A with an oxygen-deficient glue at one end of the straight rod 31A. Thereby, the magnetic block 33 is completely hidden in the straight rod 31A and is not exposed outside the straight rod 31A, thereby protecting the brittle magnetic block 33 from collision. If the collision is inadvertently impacted, the impact force is also directly The rod 31A is received to protect the magnet block 33 from damage. On the other hand, although the magnet block 33 is hidden inside the straight rod 31A, its magnetic force can still be exerted, and the magnetic rod can be attracted by penetrating the straight rod 31A.
此外，由於本創作上述之結構配置，該磁棒３結構更可達外徑細小化之目的。細言之，於本實施例中，藉由該磁塊３３形成圓柱狀且將其完全填塞於該直棒３１Ａ之內孔３２Ａ中，令該磁棒３結構整體維持足夠的結構強度，並據此可將該直棒３１Ａ之直徑細小化至2公厘至3.7公厘。相較於習知之磁棒結構，如第４圖對照於第９圖所示，本創作之磁棒３結構與隔絕管４之管徑之比例已縮小，故於操作上該磁棒３結構伸入隔絕管４的過程中因稍有歪斜而發生碰撞的機率降低，延長磁棒３結構的使用壽命。In addition, due to the above-described structural configuration of the present creation, the structure of the magnetic bar 3 can achieve the purpose of miniaturizing the outer diameter. In detail, in the embodiment, the magnetic block 33 is formed into a cylindrical shape and completely filled in the inner hole 32A of the straight rod 31A, so that the structure of the magnetic rod 3 as a whole maintains sufficient structural strength, and This can reduce the diameter of the straight rod 31A to 2 mm to 3.7 mm. Compared with the conventional magnetic rod structure, as shown in Fig. 4, as shown in Fig. 9, the ratio of the structure of the magnetic rod 3 of the present invention to the diameter of the insulating tube 4 has been reduced, so that the structure of the magnetic rod 3 is operationally extended. In the process of entering the insulation tube 4, the probability of collision due to slight skew is reduced, and the service life of the structure of the magnetic rod 3 is prolonged.
而第５圖所示者為本創作之第二實施例。本實施例包括有一直棒３１Ｂ，該直棒３１Ｂ為一實心結構體，其一端凹設有一內孔３２Ｂ，而該內孔３２Ｂ中固設有一呈圓柱狀之磁塊３３；其中於本實施例中，該磁塊３３以缺氧膠固定於該直棒３１Ｂ之內孔３２Ｂ中。藉此，該磁塊３３完全藏於該直棒３１Ｂ中而不顯露於直棒３１Ｂ外，藉以保護質地較脆之磁塊３３不受碰撞，若不慎遭受碰撞，其衝擊力量亦由該直棒３１Ｂ承受，以保護磁塊３３不受損壞。另一方面，雖然該磁塊３３藏於該直棒３１Ｂ之內部，惟其磁力仍可發揮，可穿透該直棒３１Ｂ而吸附磁珠。The figure shown in Fig. 5 is the second embodiment of the creation. The present embodiment includes a straight bar 31B, which is a solid structure body having an inner hole 32B recessed in one end thereof, and a cylindrical magnetic block 33 is fixed in the inner hole 32B; The magnetic block 33 is fixed in the inner hole 32B of the straight rod 31B with an anoxic glue. Thereby, the magnetic block 33 is completely hidden in the straight rod 31B and is not exposed outside the straight rod 31B, so as to protect the magnetic block 33 which is more brittle from impact, and if the collision is inadvertently impacted, the impact force is also The rod 31B is subjected to protect the magnet block 33 from damage. On the other hand, although the magnet block 33 is hidden inside the straight rod 31B, its magnetic force can still be exerted, and the magnetic rod can be attracted by penetrating the straight rod 31B.
此外，由於本實施例上述之結構配置，該磁棒結構更可達外徑細小化之目的。細言之，於本實施例中，藉由該磁塊３３形成圓柱狀且將其完全填塞於該直棒３１Ｂ之內孔３２Ｂ中，令該磁棒結構整體維持足夠的結構強度，並據此可將該直棒３１Ｂ之直徑細小化至2公厘至3.7公厘。相較於習知之磁棒結構，其與隔絕管之管徑之比例已縮小，故於操作上該磁棒結構伸入隔絕管的過程中因稍有歪斜而發生碰撞的機率降低，延長磁棒結構的使用壽命。In addition, due to the above-described structural configuration of the embodiment, the magnetic rod structure can achieve the purpose of miniaturizing the outer diameter. In detail, in the embodiment, the magnetic block 33 is formed into a cylindrical shape and completely filled in the inner hole 32B of the straight rod 31B, so that the magnetic rod structure as a whole maintains sufficient structural strength, and accordingly The diameter of the straight rod 31B can be reduced to 2 mm to 3.7 mm. Compared with the conventional magnetic rod structure, the ratio of the diameter to the diameter of the insulating tube has been reduced, so that the probability of collision due to slight skew during the operation of the magnetic rod structure into the insulating tube is reduced, and the magnetic rod is extended. The service life of the structure.
惟，以上實施例之揭示乃用以說明本創作，並非用以限制本創作，故舉凡等效元件之置換仍應隸屬本創作之範疇。However, the disclosure of the above embodiments is intended to illustrate the present invention and is not intended to limit the present invention, so the replacement of equivalent elements should still be within the scope of this creation.
綜上所述，可使熟知本項技藝者明瞭本創作確可達成前述目的，實已符合專利法之規定，爰依法提出申請。In summary, it can be made clear to the skilled person that the creation can achieve the aforementioned objectives, and it has already met the requirements of the Patent Law and submitted an application according to law.
1‧‧‧試劑槽 1‧‧‧Reagent tank
12‧‧‧試管空間 12‧‧‧Test tube space
2‧‧‧磁吸結構 2‧‧‧Magnetic structure
3‧‧‧磁棒 3‧‧‧Magnetic rod
31A‧‧‧直棒 31A‧‧‧ straight rod
33‧‧‧磁塊 33‧‧‧Magnetic blocks
4‧‧‧隔絕管 4‧‧‧Isolation tube
Claims (4)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW103208224U TWM485907U (en) | 2014-05-12 | 2014-05-12 | Magnetic bar structure of magnetic bead nucleic acid extracting detection system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW103208224U TWM485907U (en) | 2014-05-12 | 2014-05-12 | Magnetic bar structure of magnetic bead nucleic acid extracting detection system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| TWM485907U true TWM485907U (en) | 2014-09-11 |
Family
ID=51944612
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TW103208224U TWM485907U (en) | 2014-05-12 | 2014-05-12 | Magnetic bar structure of magnetic bead nucleic acid extracting detection system |
Country Status (1)
| Country | Link |
|---|---|
| TW (1) | TWM485907U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI695731B (en) * | 2018-11-09 | 2020-06-11 | 開啟基因股份有限公司 | Method and device of automatic nucleic acid extraction |
| TWI765296B (en) * | 2020-07-15 | 2022-05-21 | 致茂電子股份有限公司 | Magnetic bar module with multiple stages and apparatus for extracting nucleic acid having the same |
-
2014
- 2014-05-12 TW TW103208224U patent/TWM485907U/en not_active IP Right Cessation
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI695731B (en) * | 2018-11-09 | 2020-06-11 | 開啟基因股份有限公司 | Method and device of automatic nucleic acid extraction |
| TWI765296B (en) * | 2020-07-15 | 2022-05-21 | 致茂電子股份有限公司 | Magnetic bar module with multiple stages and apparatus for extracting nucleic acid having the same |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| TW200923097A (en) | Automated system and method for processing genetic material | |
| JP2020190560A (en) | System and method for collecting nucleic acid sample | |
| US9006420B2 (en) | Method for concentrating and isolating biomolecules or viruses | |
| CN103820431A (en) | Nucleic acid extraction and purification method based on nanometer magnetic beads and kit | |
| JP2009072774A5 (en) | ||
| CN101665785A (en) | Method for extracting and purifying nucleic acid from samples by magnetic beads | |
| CN107779389B (en) | Nucleic acid extraction amplification device and method of use thereof | |
| CN109182335A (en) | A kind of Viral nucleic acid extraction reagent box | |
| KR101755563B1 (en) | Kit and automatic apparatus for isolating or purifying nucleic or various biological materials | |
| CN109762874B (en) | Nucleic acid sedimentation aid, pregnant woman plasma free DNA extraction kit and method | |
| CN104694382A (en) | Rapid nucleic acid extraction device and method | |
| TWM485907U (en) | Magnetic bar structure of magnetic bead nucleic acid extracting detection system | |
| CN103013814B (en) | Nucleic acid purification device | |
| CN106660043B (en) | Device and method for preparing cell nucleus | |
| TWM467512U (en) | Reagent vessel and kit thereof | |
| CN116121237A (en) | Magnetic bead method alcohol-free nucleic acid extraction kit and extraction method thereof | |
| WO2012103214A2 (en) | Magnetic pipette tip | |
| CN107201360A (en) | One kind is used for virus gene genome nucleic acid rapid extraction reagent method | |
| CN203855573U (en) | Magnetic rod structure of magnetic bead extraction nucleic acid detection system | |
| CN105021835B (en) | A kind of Magnetic driving biological specimen processing meanss and its processing method | |
| JP2013500742A (en) | Method and device for recovering microbial nucleic acids from particulate samples | |
| US20220326126A1 (en) | Detergent-free simultaneous multiomics sample preparation method using novel new vesicle design | |
| RU2451747C2 (en) | Method for homogenising biological samples containing magnetic nanoparticles when extracting dna during automatic sample preparation for pcr analysis | |
| CN106916812A (en) | A kind of paramagnetic particle method Ago-Gel DNA extraction kit and its extracting method | |
| JP2019037159A (en) | Cell content recovery method and cell content recovery apparatus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MK4K | Expiration of patent term of a granted utility model |