M274953 【新型所屬之技術領域】 本I丨作為_ 3甲殼醣之敷料，特別是本創作利用游 離輪射、UV光照射法或冷;東乾燥法，將丙婦酸、異丙基丙烯 =及聚:㈣接枝以在基材上製成敷材，可應用於燒烫. 傷敷料、抗翻材料。 m 【先前技術】 φ 目前藉由光照射接枝聚合法在塑膠工業上已被廣泛使 用^湘均等於八十三年醫工研討會（ 1995,284.)發表「醫 用向分子表面接枝聚合感溫性N|pAAm膜」，以uv光昭射 接枝NIPAAm於高分子醫用薄膜上以改善濕滑性並將發展 應用於醫用導管；Η. T. L〇khande等人於八十—年發表使用 Y幸田射照射接枝丙烯睛（aC「y|jc m.tr丨丨e)於塑膠上（H 丁 L〇khande etc·，」.〇f App丨 p〇|y Sd 48 495 1993 )。按，· :般習用者’如中華民國專利公報公告第4 5 8 7 8 4號之鲁 「易剝離性創傷敷料之製法及由此法製得之敷料」，其係利 用r-射線輕射照射不織布，使發生表面接枝共聚合，其反應 係以始-60放射高能量7_射線使不織布產生自由基或過氧_ 化物，並與異丙基丙烯醯胺單體接枝於不織布上，其因不織· 布本身沒有殺ϋ的效果，反时助長細g的生長，雖含有丙 =酸與異丙基丙稀g!胺後，則具有殺g效果，但未能達到所 需之效果》且由於含有異丙基丙烯醯胺層導致形成一緻密薄 6 M274953 層阻礙了水氣的透過，造成其水氣透過速率、穿透性，和透 氣性都會相對降低而變差。 【新型内容】 本創作之主要目的係在於提供一具抗菌性、親水性、溫 度敏感性的含聚甲殼之敷材。 為達上述之目的，本創作係提供一種含聚甲殼醣之敷 料，係利用游離輻射或uv光照射法將一丙烯酸或丙烯酸 =異丙基丙稀醯胺接枝固定在一基材表面上，形成一丙烯 酸或丙烯酸及異丙基丙烯醯胺層，再將一聚甲殼醣利用游 離輻射、UV光或冷凍乾燥法接枝固定在該丙烯酸或含丙 烯酸及異丙基丙烯醯胺層上，再形成一聚甲殼醣層，由於 臈1變厚，使其結構為緻密及多孔型，最後將具殺菌性的 物質（如·· Ag、Zn、AI)噴灑在含聚甲殼之敷料表面上增加 其杬菌性，得一具抗菌性、親水性、溫度敏感性等多功能 之含聚曱殼醣之敷料，可應用在燒燙傷敷料、抗菌材料。 M274953 【實施方式】 請參閱『第1A〜1B圖』所示，係本創作之含聚曱殼 醣之敷料剖面示意圖、本創作之另一含聚曱殼醣之敷料剖面 示意圖。如圖所示：係提供一種含聚曱殼之敷料，係使用一 以不織布（如：聚乙烯，聚丙烯，PET，cotton等）、PVC布及 ‘ 其它各種布料與薄膜狀或平板狀的材料（如：高分子，纖維素 ， 等）為基材1，及一不同混合比例（1:0、1:1或3:1)的丙烯酸 與異丙基丙烯醯胺（NIPAAm)或其類似物單體〔（乙烯基類反· 應性單體），如··丙烯酸、異丙基丙烯醯胺和它的衍生物、曱 基丙烯酸-2-羥基乙酯、甲基丙烯酸-2-羥基乙酯和它的衍生 物、乙烯基吡啶、乙烯基吡啶和它的衍生物〕、水膠類（如： 丙烯酸、甲基丙烯酸-2-羥基乙酯），利用游離輻射或UV光 照射法將該丙烯酸與異丙基丙烯醯胺固定在該基材1上，形 成一丙烯酸與異丙基丙烯醯胺層2，再將一聚曱殼醣利用游 離輻射或UV光照射法接枝固定在該丙烯酸與異丙基丙烯醯 胺層2上’形成一聚曱殼醣層3，最後噴灑上AgN〇3溶液，φ 即得一含聚甲殼之敷料。 另，本創作亦可提供另一種含聚甲殼之敷料，係將一基 材1以伽瑪射線照射或UV光照射法進行輻射照射將一以乙 醇為溶劑，比例為30%(V/V)之丙烯酸或其類似物單體（如··-曱基丙烯酸、烯丁酸 '己烯酸）固定在該基材i上，形成一丙 烯酸層4。照射後以乙醇和丙酮將該丙烯酸層4上未固定之 丙烯酸單體去除。將該丙烯酸層4以一硫酸與過氧化氫進行 8 M274953 過氧酸化過程，形忠—1 t a 在該過氧酸根# ^—欠认根層5，再以冰甲醇去除殘餘 . 上的硫醆與過氧化氫，將該過氧酸根層5 场離騎照射法或uv光照射 烯醯胺接枝固定在該過氧酸栌居“仃…m、丙基丙 胺層6。再將-聚甲=0層c5t’形成一異丙基丙_ 在 196°c下進行冷凌乾燥過 使^曱㈣接枝固定在該異丙基丙雜胺層6上，再 形成-聚甲殼醣層3 ’即完成_含聚甲殼之敷料。 為進^ 11兒月本創作，本創作係進一步以數個較佳實施 例說明如後： [實施例1]丙烯酸與聚甲殼醣以uv光照射法照射接枝 固定在不織布上： 请參閱『第2 A〜3 B圖』所示，係本創作之含聚甲技 醣之敷料AN10C剖面示意圖、本創作之未處理前不織布（p^) 戴面的電子顯微鏡（SEM)照片示意圖、本創作之含聚甲殼酿 之敷料AN10C截面的電子顯微鏡（SEM)放大15〇倍照片示 意圖、本創作之含聚曱殼醣之敷料AN 10C戴面的電子顯微 鏡（SEM)放大600倍照片示意圖。如圖所示··係使用不織 布為基材1 (pp，8cmx8cm)，一以乙醇為溶液，比例為 10%(V/V)且含有小於5wt°/。的苯偶姻***當光起始劑之丙稀 酸，利用UV光照射法將該丙稀酸接枝在該不織布上，其 射時間10〜100分，照射後形成一丙烯酸層4，再將—以 M274953 0.1M乙酸為溶劑，溶液濃度為，溶液甲含有小於 5 w t %的苯偶姻***當作光起始劑之聚曱殼醣，利用口 v光照 射法照射10〜1〇〇分，使該聚甲殼醣固定在該丙烯酸層4 上，形成一聚甲殼醣層3。 [貫施例2]丙烯酸、異丙基丙烯醯胺與聚甲殼醣溶液以· UV光照射法照射固定在不織布上： 請參閱『第4A、第4B圖』所示，係本創作之含聚甲 殼醣之敷料AN11C截面的電子顯微鏡（SEM)放大15〇倍照 片示意圖、本創作之含聚甲殼醣之敷料AN11C截面的電子 顯微鏡（SEM)放大600倍照片示意圖。如圖所示：係使用 不織布為基材1 ( PP，8cmx8cm)及一以丙烯酸（1〇〇/〇 (v/v)，溶於乙醇〕與異丙基丙烯醯胺（〇148gNMPAAm/ 1ml乙醇〕混合比例為1n (v/v)並含有小於5wt%的苯偶 姻***為光起始劑之丙烯酸與異丙基丙烯醯胺，將該丙烯酸籲 與異丙基丙烯醯胺以UV光照射1〇〜1〇〇分固定在該不織布 表面上，照射後形成一丙烯酸與異丙基丙烯醯胺層2，再以 一 〇·1 Μ乙酸為溶劑，溶液濃度為Uwty。，溶液中含有小 於5wt%的笨偶姻***當作光起始劑之聚曱殼醣，以uv光· 照射10〜100分接枝固定在該丙烯酸與異丙基丙烯醯胺層2 上，形成一聚曱殼醣層3。 [實施例3】丙烯酸、異丙基丙烯醯胺與聚曱殼醣以加馬 M274953 射線，UV光照射與過氧酸化過程與冷康乾燥法固定在不織 布上： 清參閱『第5 A、第5 B圖』所示，係本創作之含聚曱 殼醣之敷料PP-nipga-chio截面的電子顯微鏡（SEM)放大 40倍照片示意圖、係本創作之含聚甲殼醣之敷料 PP-nipga-chio截面的電子顯微鏡（SEM)放大15〇倍照片示 意圖。如圖所示：係以不織布（8cmx8cm)為基材丄，將一丙 稀酸（30〇/〇 (V/V)，溶於乙醇〕以加馬射線照射進行照射， 照射時間為0.5〜1 0小時，照射劑量率為o n 〇Gy/h「，總照 射劑量約5〜嫩Gy，使其接枝固定在該不織布上，形成二丙 烯酸層4，照射後以乙醇和丙酮為清洗劑。再將該丙歸酸層 4之表面以一硫酸與過氧化氫，於〇ΐ下進行過氧酸化過 :’反應時間0.5〜6小時，形成一過氧酸根層5。反應後以 冰甲醇去除殘餘該過氧酸根層5上的硫酸與過氧化氫。以一 :丙基丙烯醯胺(請乙酸為溶劑)進行υν光照射制〇〇 刀’將該異丙基丙烯醯胺固定於該過氧酸根 異丙基丙烯醯胺薄層6。在以一 〇 1Μ 于 ^Λ 1 ς +0/ ^ · Μ乙1為>谷劑，溶液濃 為二了/。’亚加入1mMwt%戊二駿之聚甲殼聽於π 酿胺層6上，形成接枝〜在料丙基丙稀 [貫施例4】丙烯酸、里而其兩** 基丙婦酿胺與聚甲殼醣健以 加馬射線’υν先照射與過氧酸化法與冷心::; M274953 織布上: 請參閱『第6A11、第6Βϋ』所示，係本創作之含聚 甲殼醣之敷料PP_nipga_c刚截面的電子顯微鏡(sem)放 大40倍照片示意圖、本創作之含聚甲殼醣之敷料_ PP-nipga-chiN截面的電子顯微鏡（SEM)放大15〇倍照片示· 意圖。如圖所示：係指將_不織布為基材丄，並與一丙稀酸 (30% (V/V)’溶於乙醇〕’以加馬射線照射進行照射，照射· 寺門為0·5 1 〇小時，照射劑量率為〇 $〜1 〇Gy/hr，總照射劑 量約5〜60kGy，將該丙烯酸固^在該不織布上，形成一丙稀 酸層4 ’照射後以乙醇和丙料清洗劑，洗去該丙稀酸層4 上未固定之丙烤酸單體。將該丙烯酸層4與一體積比例為 1:9之硫酸與過氧化氫，於〇°C下進行過氧酸化過程，反應 1間0.5〜6小時’形成—過氧酸根層5，並在反應後以冰甲 醇洗去殘餘該過氧酸根層5之表面上的硫酸與過氧化氮。將 了異丙基丙婦醯胺（〇_1M乙酸為溶劑）與該過氧酸根層5以 一先照射10〜⑽分以得一異丙基丙稀醯胺薄層6。將以 一 〇.1M乙酸為溶劑，溶液濃度為卜5wt%，並加人1mMwt% 戊-搭溶液之聚甲殼酶，在_196t下冷;東24小時，再進行· 2乾燥過程’使該聚甲殼酿接枝固定在該異丙基丙稀酿胺 層6上，形成一聚甲殼醣層3。 a 月，閱表1〜3』所示，係本創作之含聚甲殼醣 文料的相f子吸水時間與溫度敏感性測試表、本創作之含聚 12 M274953 甲/Λ醣之敷料的殺菌測試結果表、本創作之含聚甲殼酶之敷 料的水氣透過測試表。如表1所示，可知隨著丙稀酸的含量 減少，異丙基丙烯醯胺的含量增加，其相對吸水時間增加， 而經聚甲殼酶接枝在丙烯酸與異丙基丙烯㈣層2上後，其 吸水時間相對減少’顯示其親水性增加。該表k 卜、 AN11 ’不織布經異丙基丙稀醯㈣定接枝後，在彳5、耽 夺親大’在41、53°C時親水性較小，可知該不織布 經固，接枝後具有溫度敏感性。另，如表2所示，可知經由 光”、、射法處理後’含丙烯酸、異丙基丙稀醯胺與聚甲殼 酶的不織布具有較佳的㈣絲，如該表2數值顯示，不織 布本身不會殺g反而會促進細g生長，*當該不織布含有丙 稀，與異丙基丙烯醯胺後’則具有殺®效果，能降低細菌的 數！。在聚曱殼醣.岐接枝在含有丙烯酸、異丙基丙稀酿胺 ^識布上後，更進-步的大量降低細菌的數量，顯示其殺菌 效果增加。如表3所示，可知隨著丙烯酸的含量減少，異丙 基丙_胺的含量增加，其水氣透過速率、穿透性，和透氣 性都呈現下降的趨勢，是由於丙稀酸比異丙基丙稀醯胺較吸 水’故當丙稀酸的量減少時’同時減少其親水性與水氣透氣 性。經聚甲殼醣接枝固定在丙烯酸與異丙基丙烯醯胺層2上 後由於其膜厚、交的較大使其結構較緊密，阻礙了水氣的透 過’所以水氣透過速率、穿透性’和透氣性都呈現下降的趨 勢。 請參閱『表4〜7』所示，係本創作之含聚甲殼醣之敷 M274953 料的相對吸水時間表、本創作之含聚曱㈣之敷料的溫度敏 感生K表、另—本創作之含聚曱殼餹之敷料的殺菌測試結 果表另本創作之含聚曱殼醣之敷料的水氣透過測試表。 ^表4所7F ’可知不織布經丙烯酸和異丙基丙烯醯胺接枝固 ，後其吸水時間減少，顯示其親水性有增加。在含有聚甲 殼醋其吸水時間大幅下降，顯示其親水性又進—步的增 ^ ’是由於聚甲殼酶十分具有親水性，能快速吸取大量水 刀，故在合有聚甲殼醣後能大幅增加不織布的親水性質。另 外’比較以不同冷;東溫度所製成的含聚甲殼醣之不織布，可 發，在-196Ϊ時所製做出的含聚f㈣之敷料，其吸水時間 較高。如第4 A〜5 B圖所示，可知在_19代時所製做出的 含聚甲殼醣之敷料之結構較密實，其密實的結構會使水較難 進^該含有聚曱殼酶之敷料裏。如表5所示，可知在15、 M C N·合有異丙基丙烯醯胺層6，其吸水時間比在38、 C夺J .·，、員示其在15、25 C時之親水性較好，而在38、53 c夺之親水差，亦顯示該異丙基丙稀醯胺層6具有溫度 敏感性，達到利用溫度的變化而改變其親水性之目的。如二 6所示，可知不織布本身沒有殺菌的效果，反❿會助長細菌 的生長。在含有異丙基丙稀醯胺層6後，其殺菌效果略有增 加。經聚甲殼醣接枝固定後，由於聚甲殼醣本身具有殺菌性 之效果’使其殺菌效果更進-步的增加。如表7所示，可知 在含有異丙基丙烯醯胺層6後，其水氣透過速率、穿透性， 和透氣性都呈現下降的趨勢，是由於該異丙基丙雜胺層6 形成一緻密薄層阻礙了水氣的透過所造成的。經聚甲殼醣接 14 M274953 =固，因聚甲殼醣具有較大的親水性’其水氣透過速 率、牙透性，和透氣性都呈現上升的趨勢。另一方面 冷輕度所製成的含聚甲㈣之敷料，可發現在⑽ c時所製做出的含聚甲殼醣之敷料， …8』所一二= 胺之不織布的細囷阻隔能力測試。如表所示， :甲殼聽之敷料經固定接枝後都含有 、酿胺： 二異丙基丙稀醯胺層6之不織布做細菌= 基丙後’仍無細菌穿過該含有異丙 不織布對阻擒細二 請參閱『第7圖』所示 圖所-係指在含聚甲殼之敷料表面上嘴不如 液，如：Aq、Δ, π Έ上3金屬離子之溶 性。如該圖所示，、以ΑΝ3=自然陰乾，以增加敷料的抗菌 聚甲〜Μ 錢Α9Ν〇3溶液為例，在守含 ::戒之敷料周圍有一空白的無白 ^ “ 殼之敷料具有抗菌的效果。由該匕 含有銀離子之X祕—N T J有出，可知 之不織布的殺菌效果最好。 惟以上所述者，僅為本創 以此限定本創作實施 車乂佳貫把例而已，當不能 及創作說明書内容所:二本創作申請專利範圍 本創作專利涵蓋之範圍内。*政-又化與修飾，皆應仍屣 M274953 【圖式簡單說明】 第1A圖’係本創作之含聚甲殼醣之敷料剖面示意圖； 第1 B圖’係'本創作之另—含聚甲殼之敷料剖面示意 圖； 第2八圖，係本創作之含聚甲殼醣之敷料AN1GC剖面示 意圖； 第2 B圖’係本創作之未處理前不織布（PP)截面的電子顯 微鏡（SEM)照片示意圖； 第3 A圖’係本創作之含聚曱殼醣之敷料an 1 〇c截面的 電子顯微鏡（SEM)放大150倍照片示意圖； 第3 B圖’係本創作之含聚曱殼醣之敷料ani〇c戴面的 電子顯微鏡（SEM)放大600倍照片示意圖； 第4 A圖，係本創作之含聚曱殼醣之敷料an 11 C戴面的 電子顯微鏡（SEM)放大150倍照片示意圖； 第4 B圖，係本創作之含聚曱殼醣之敷料AN11C戴面的 電子顯微鏡（SEM)放大150倍照片示意圖； 第5 A圖’係本創作之含聚曱殼醣之敷料pp-njpga-Ch|〇 M274953 戴面的電子顯微鏡（sem)放大40倍照片示意 圖； 第5 B圖’係本創作之含聚曱殼醣之敷料pp nipga-ch丨〇 戴面的電子顯微鏡（SEM)放大150倍照片示 意圖；M274953 [Technical field to which the new type belongs] This I 丨 is used as a dressing for _3 chitosan, especially this creation uses free wheel irradiation, UV light irradiation method or cold; East drying method, the acetic acid, isopropyl propylene = and Poly: ㈣ grafted to make a dressing on the substrate, which can be used for burns. Wound dressings, anti-turning materials. m [Prior technology] φ At present, the graft polymerization method by light irradiation has been widely used in the plastic industry. ^ Hunan is equal to 83 years of medical industry seminar (1995, 284.). Polymerization of thermosensitive N | pAAm film ", grafting NIPAAm on polymer medical film with UV light to improve wet slip and apply it to medical catheters; Η. T. Lokhande et al., 80 —Announced the use of Y Koda shots to irradiate acryl (aC "y | jc m.tr 丨 丨 e") onto plastic (H 丁 L〇khande etc., ". 〇f App 丨 p〇 | y Sd 48 495 1993). Press, ·: General users, such as the "Method for Producing Peelable Wound Dressings and Dressings Prepared by This Method", such as Lu of the Republic of China Patent Gazette Bulletin No. 4 5 8 7 8 4, which is irradiated with light by r-rays. Non-woven fabrics cause surface graft copolymerization. The reaction is to irradiate high-energy 7-rays from -60 to make the non-woven fabrics generate free radicals or peroxides, and graft with isopropylacrylamide monomers on the non-woven fabrics. The non-woven cloth does not have a killing effect, and it promotes the growth of fine g. Although it contains acrylic acid and isopropylpropylglyceride, it has a killing effect, but it fails to achieve the desired effect. "Effectiveness" and the formation of a uniformly dense 6 M274953 layer due to the isopropylacrylamide layer impedes the permeation of water vapor, causing its water vapor transmission rate, permeability, and air permeability to be relatively reduced and deteriorated. [New content] The main purpose of this creation is to provide a anti-bacterial, hydrophilic, and temperature-sensitive dressing containing polycarapace. In order to achieve the above purpose, this creation is to provide a dressing containing polychitosan, which is grafted and fixed on the surface of a substrate with acrylic acid or acrylic acid = isopropyl propylamine using free radiation or UV light irradiation. A layer of acrylic acid or acrylic acid and isopropylacrylamide is formed, and then a chitosan is grafted and fixed on the acrylic or acrylic acid and isopropylacrylamide layer by free radiation, UV light or freeze drying, and then A polychitosan layer is formed. As 臈 1 thickens, its structure is dense and porous. Finally, bactericidal substances (such as Ag, Zn, AI) are sprayed on the surface of the polychitosan-containing dressing to increase its thickness. It is fungicidal and obtains a multifunctional chitosan-containing dressing with antibacterial, hydrophilic, and temperature sensitivity functions, which can be applied to burns and antibacterial materials. M274953 [Embodiment] Please refer to "Figures 1A ~ 1B", which is a schematic cross-sectional view of a dressing containing polysaccharide in this creation, and a schematic cross-sectional view of another dressing containing polysaccharide in this creation. As shown in the figure: It provides a dressing containing polycondensed shell, which uses a non-woven cloth (such as: polyethylene, polypropylene, PET, cotton, etc.), PVC cloth, and other various cloths and film or flat materials. (Such as: polymer, cellulose, etc.) as the base material 1, and a different mixing ratio (1: 0, 1: 1, or 3: 1) of acrylic acid and isopropylacrylamide (NIPAAm) or the like Monomers [(vinyl-based reactive monomers), such as acrylic acid, isopropylacrylamide and its derivatives, 2-hydroxyethyl methacrylate, 2-hydroxyethyl methacrylate Esters and its derivatives, vinylpyridine, vinylpyridine and its derivatives], hydrocolloids (such as: acrylic acid, 2-hydroxyethyl methacrylate), using free radiation or UV light irradiation Acrylic acid and isopropylacrylamide are fixed on the substrate 1 to form an acrylic acid and isopropylacrylamide layer 2, and then a polyfluorene chitosan is grafted and fixed on the acrylic acid by free radiation or UV light irradiation. Form a polyfluorene chitosan layer 3 on the isopropylacrylamide layer 2 and finally spray with AgNO. , Φ to obtain a polyethylene-containing dressing of the crustaceans. In addition, this creation can also provide another dressing containing polycarapace. A substrate 1 is irradiated with gamma rays or UV light, and ethanol is used as a solvent at a ratio of 30% (V / V). Acrylic acid or its analog monomers (such as ...- fluorenyl acrylic acid, butyric acid 'hexenoic acid) are fixed on the substrate i to form an acrylic layer 4. After the irradiation, the unfixed acrylic monomer on the acrylic layer 4 was removed with ethanol and acetone. The acrylic layer 4 was subjected to a peroxy acidation process with a sulfuric acid and hydrogen peroxide 8 M274953, the form of zhong 1 ta in the peroxy acid radical # ^-underrecognized root layer 5, and then the residual thiosulfate with ice methanol. With hydrogen peroxide, the peroxyacid layer was irradiated with 5-field off-iron irradiation or UV light irradiation, and was fixed on the peroxyacid layer "仃 ... m, propylpropylamine layer 6. Then -polymethylamine = 0 layer c5t 'forms an isopropyl propyl_ It is subjected to chilling and drying at 196 ° C, and ^ 曱 ㈣ is grafted and fixed on the isopropylpropylamine layer 6 to form -polychitosan layer 3 ′ That is to say _ dressing containing polychitosan. In order to develop this 11 months, this creation is further explained with several preferred embodiments as follows: [Example 1] Acrylic acid and polychitosan were irradiated with UV light. The branch is fixed on the non-woven fabric: Please refer to "Figures 2 A ~ 3 B", which is a schematic cross-sectional view of the dressing AN10C containing polysaccharides, and the untreated non-woven fabric (p ^) of this creation. Microscope (SEM) photo schematic diagram, the electron microscopy (SEM) magnification of the cross-section of the dressing AN10C containing the poly-crust beer of this creation is 150 times. A schematic illustration of a 600 times magnified electron microscope (SEM) image of the AN-10C wearing dressing containing polysaccharide containing chitosan. The picture shows the use of a non-woven fabric as the base material 1 (pp, 8cmx8cm), ethanol It is a solution with a proportion of 10% (V / V) and containing less than 5wt ° /% of benzoin ethyl ether as the acrylic acid of the photoinitiator. The acrylic acid is grafted onto the nonwoven fabric by UV light irradiation. , Its irradiation time is 10 ~ 100 minutes, an acrylic layer 4 is formed after irradiation, and then—with M274953 0.1M acetic acid as the solvent, the solution concentration is, the solution A contains less than 5 wt% benzoin ether as a photoinitiator The chitosan is irradiated with 10 to 100 minutes by the v-ray irradiation method, so that the chitosan is fixed on the acrylic layer 4 to form a chitosan layer 3. [实施 例 2] ACRYLIC, ISO The solution of propylacrylamide and polychitosan was fixed on the non-woven fabric by UV light irradiation: Please refer to "Figures 4A and 4B". This is an electron microscope section of the chitosan-containing dressing AN11C. (SEM) Schematic of a 150x magnified photo, cut of AN11C with chitosan-containing dressing SEM image of 600 times magnification. As shown in the figure: the non-woven fabric is used as the substrate 1 (PP, 8cmx8cm) and acrylic (100 / 〇 (v / v), soluble in ethanol) and Isopropylacrylamide (〇148gNMPAAm / 1ml ethanol) acrylic acid and isopropylacrylamide with a mixing ratio of 1n (v / v) and containing less than 5wt% benzoin ether as a photoinitiator. Call for isopropylacrylamide to be fixed on the surface of the non-woven fabric by UV light irradiation for 10 to 100 minutes. After the irradiation, an acrylic acid and isopropylacrylamide layer 2 was formed, and then 0.1 μM acetic acid was used as Solvent with a solution concentration of Uwty. The solution contains less than 5wt% polyammonium ether as a photoinitiator, and polyfluorene chitosan is grafted and fixed on the acrylic acid and isopropylacrylamide layer 2 with UV light and 10 ~ 100 branch irradiation. A polyfluorene shell sugar layer 3 is formed. [Example 3] Acrylic acid, isopropylacrylamide, and chitosan were fixed on a non-woven fabric by Gama M274953 radiation, UV light irradiation, peroxyacidification process, and cold-kang drying method: refer to "5A, As shown in Figure 5B ", this is a schematic diagram of a magnification of 40 times by an electron microscope (SEM) of the section of the dressing PP-nipga-chio containing polycarminose, and the dressing PP-nipga- Electron microscope (SEM) magnification of the chio cross section at 150 times. As shown in the figure: Non-woven cloth (8cmx8cm) is used as the base material. Monopropionic acid (30/0 (V / V), dissolved in ethanol) is irradiated with gamma rays. The irradiation time is 0.5 ~ 1. At 0 hours, the irradiation dose rate was on 〇Gy / h, and the total irradiation dose was about 5 to tender Gy, which was grafted and fixed on the non-woven fabric to form a diacrylic layer 4. After the irradiation, ethanol and acetone were used as cleaning agents. The surface of the propionate acid layer 4 was subjected to peroxyacidification with monosulfuric acid and hydrogen peroxide at 0 ° C: the reaction time was 0.5 to 6 hours to form a peroxyacid layer 5. After the reaction, the residue was removed with ice methanol. Sulfuric acid and hydrogen peroxide on the peroxyacid layer 5. A: propylacrylamide (please use acetic acid as a solvent) was irradiated with νν light to prepare 〇k 'to fix the isopropylacrylamide to the peroxy Acid isopropyl acrylamidine thin layer 6. In the presence of 010M to ^ Λ 1 ς + 0 / ^ · M ethyl 1 as > cereal, the solution was concentrated to 2%. 'Asia added 1mMwt% glutarium The polychitosan was grafted on the π-fermented amine layer 6 to form a graft. In the case of propyl propyl acrylate [4. Chitosan Jian with gamma rays' υν first irradiation and peroxyacid method and cold heart ::; M274953 On the woven fabric: Please refer to "6A11, 6Bϋ", this is a dressing containing chitin PP_nipga_c Schematic diagram of 40 times magnification of the electron microscope (sem) with a rigid cross section. The chitosan-containing dressing _ PP-nipga-chiN cross section of the electron microscope (SEM) with a magnification of 150 times. Refers to _ non-woven fabric as the base material, and irradiated with mono-acrylic acid (30% (V / V) 'soluble in ethanol]' with gamma rays, irradiation · Temple door for 0.5 10 hours, irradiation The dose rate is 0 to 100 Gy / hr, and the total irradiation dose is about 5 to 60 kGy. The acrylic acid is fixed on the non-woven fabric to form an acrylic acid layer. 4 'After irradiation, wash with ethanol and acrylic cleaning agent. The acrylic acid monomer is not fixed on the acrylic acid layer 4. The acrylic acid layer 4 and a sulfuric acid and hydrogen peroxide having a volume ratio of 1: 9 are subjected to a peroxy acidification process at 0 ° C for 1 reaction. 0.5 ~ 6 hours' formation-peroxyacid layer 5 and after the reaction, the surface of the remaining peroxyacid layer 5 is washed with ice methanol Sulfuric acid and nitrogen peroxide. Isopropylpropanamine (0_1M acetic acid as a solvent) and the peroxyacid layer 5 were irradiated with 10 ~ 50 minutes first to obtain an isopropylpropionamine Thin layer 6. Polychitinase with 10.1M acetic acid as the solvent and a solution concentration of 5wt%, and added with 1mMwt% pentosan solution, cooled at _196t; 24 hours in the east, and then dried 2 times Process 'The polychitosan is grafted and fixed on the isopropyl propylene amine layer 6 to form a polychitosan layer 3. A month, see Tables 1 to 3', this is a polychitin containing this creation Test table for water absorption time and temperature sensitivity of sugar materials, bactericidal test result table for the poly 12 M274953 alpha / Λ sugar-containing dressing of this creation, and water vapor transmission test table for the poly-chitinase-containing dressing of this creation . As shown in Table 1, it can be seen that as the content of acrylic acid decreases, the content of isopropylacrylamide increases and its relative water absorption time increases, and the polyacrylase is grafted onto acrylic acid and isopropylacrylamide layer 2 After that, its water absorption time was relatively reduced, showing its increased hydrophilicity. According to the table, AN11 'Non-woven fabric was grafted with isopropyl propylene, and the hydrophilicity was lower at 41 and 53 ° C. It can be seen that the non-woven fabric was fixed and grafted. After having temperature sensitivity. In addition, as shown in Table 2, it can be seen that the non-woven fabric containing acrylic acid, isopropylpropylamine, and polychitinase has better reeling after being treated with light, and shot. As shown in the value of Table 2, the non-woven fabric It will not kill g, but will promote the growth of fine g. * When the non-woven fabric contains acrylic, it has a "killing effect" after isopropylacrylamide, which can reduce the number of bacteria! ... After the acrylic acid and isopropyl propylamine amines were found on the cloth, the number of bacteria was further reduced by a large amount, showing that the bactericidal effect was increased. As shown in Table 3, it can be seen that as the content of acrylic acid decreases, the The increase in the content of propyl propylamine, its water vapor transmission rate, permeability, and air permeability all show a downward trend. This is because acrylic acid is more water absorbing than isopropyl propylamine. When the amount is reduced, it also reduces its hydrophilicity and water vapor permeability. After the chitosan is grafted and fixed on the acrylic and isopropylacrylamide layer 2, its structure is tighter due to its thicker film and larger cross, which hinders it. Water vapor transmission 'so water vapor transmission rate, permeability' The air permeability shows a downward trend. Please refer to [Tables 4 to 7], which are the relative water absorption schedules of the chitosan-containing dressing M274953 and the temperature-sensitive properties of the polygadolin-containing dressing. Table K, another-the sterilization test result of the dressing containing polycarp shell crepe, the water vapor transmission test table of the dressing with polycarp shell sugar, ^ This table shows that 7F ' After propylacrylamide is grafted and solidified, its water absorption time is reduced, showing an increase in its hydrophilicity. In the case of polychitin vinegar, its water absorption time is significantly reduced, indicating that its hydrophilicity has been further increased-due to the polychitinase It is very hydrophilic and can absorb a large number of waterjets quickly, so the hydrophilic properties of nonwovens can be greatly increased after the combination of chitosan. In addition, the comparison of non-woven fabrics containing chitosan made by different temperatures; , Poly-F㈣-containing dressings made at -196Ϊ, have a higher water absorption time. As shown in Figures 4A to 5B, we can see that poly-chitosan-containing dressings made in the _19th generation Its structure is denser, its dense knot It will make water harder to get into the dressing containing polyenzyme. As shown in Table 5, it can be seen that at 15, MCN · Isopropylacrylamide layer 6 has a water absorption time ratio of 38, C .. It is shown that its hydrophilicity is better at 15, 25 C, and its poor hydrophilicity at 38 and 53 c also shows that the isopropyl propylamine layer 6 is temperature sensitive and reaches the utilization temperature The purpose of changing its hydrophilicity is shown in Figure 2. As shown in II.6, it can be seen that the non-woven fabric itself has no bactericidal effect, but it will promote the growth of bacteria. After containing the isopropylpropylamine layer 6, its bactericidal effect is slightly Increased. After the chitosan is grafted and fixed, the chitosan itself has a bactericidal effect to further increase the bactericidal effect. As shown in Table 7, it can be seen that the isopropylacrylamide layer 6 Later, its water vapor transmission rate, permeability, and air permeability all showed a downward trend, which was caused by the isopropylpropylamine layer 6 forming a uniform dense layer that prevented water vapor transmission. Connected via polychitosan 14 M274953 = solid, because chitosan has greater hydrophilicity, its water vapor transmission rate, tooth permeability, and air permeability are all increasing. On the other hand, a dressing made of chitosan containing polycaproxene can be found in the chitosan-containing dressing made at ⑽c,… 8 ”where = 12 = the fine barrier properties of non-woven non-woven fabrics test. As shown in the table, the dressings of crustacean are fixed and grafted, and the amines are made: The non-woven fabric of diisopropylpropylamine layer 6 is made of bacteria = after the propyl group, no bacteria pass through the non-woven fabric containing isopropyl For the details of the trapping, please refer to the figure shown in "Figure 7"-it means that the mouth of the dressing containing poly-shell is not as good as liquid, such as the solubility of 3 metal ions on Aq, Δ, π Έ. As shown in the figure, taking AN3 = Natural Shade Drying, as an example to increase the dressing of the antibacterial polymethan ~ Mqian A9NO3 solution, for example, there is a blank, white-free around the dressing containing :: jiezhi dressing. Antibacterial effect. From the secret of X-NTJ containing silver ions, it can be seen that the non-woven fabric has the best sterilization effect. However, the above is only used by the original to limit the creation of this example. When the content of the creation manual cannot be met: The scope of the two patents for the creation of the creation is within the scope of the creation patent. * The government-reformation and modification should still be M274953 [Simplified illustration of the diagram] Figure 1A Sectional schematic diagram of a chitosan-containing dressing; Figure 1B is 'other' of this creation—a schematic section of a chitosan-containing dressing; Figures 28 and 8 are cross-sectional schematic diagrams of a chitosan-containing dressing AN1GC; Section 2 Figure B 'is a schematic diagram of an electron microscope (SEM) photograph of a non-woven (PP) cross section of this work before creation; Figure 3 A' is an electron microscope (SEM) of an anoc OC cross section of a dressing containing polysaccharides ) 150 times magnified photo display Figure; Figure 3B 'is a 600 times magnified photograph of an electron microscope (SEM) of the dressing containing oligosaccharid containing dressing of this creation; Figure 4A is a photo of polysaccharide containing dressing Schematic diagram of 150 times magnification of the electron microscope (SEM) of the dressing an 11 C; Figure 4B is a schematic diagram of 150 times magnification of the electron microscope (SEM) of the dressing AN11C of the dressing containing polysaccharides; Fig. 5 A 'is a dressing containing polysaccharides in this work pp-njpga-Ch | 〇M274953 A schematic diagram of a magnified 40-fold electron microscope (sem) on the face; Figure 5 B' is a poly-shell incl. Sugar dressing pp nipga-ch 丨 〇 Schematic diagram of 150 times enlarged electron microscope (SEM);
第6 A圖’係本創作之含聚曱殼醣之敷料pp_nipga chjN 戴面的電子顯微鏡（SEM)放大40倍照片示意 圖； 第6 B圖’係本創作之含聚曱殼醣之敷料pp_njpga_ch丨·Ν 截面的電子顯微鏡（SEM)放大15〇倍照片示 意圖；及 第7圖’係本創作抗菌照片示意圖。 表1，係本創作之含聚曱殼醣之敷料的相對吸水時間與溫 度敏感性測試表； 表2 ’係本創作之含聚曱殼醣之敷料的殺菌測試結果表； 表3，係本創作之含聚甲殼醣之敷料的水氣透過測試表； 表4 ’係本創作之含聚曱殼醣之敷料的相對吸水時間表； 表5 ’係本創作之含聚曱殼醣之敷料的溫度敏感性測試 17 M274953 表； 表6 ’係另一本創作 之含聚曱殼醋之敷料的殺菌測試結果 表； 表7，係另一本創作之含聚曱殼醣之敷料的水氣透過測試 表；及 細菌阻隔能力 表8 ’係本創作含異丙基丙烯醯胺不織布的 測試表。 M274953 【主要元件符號說明】 基材 1 丙烯酸與異丙基丙烯醯胺層 聚甲殼醣層 3 丙稀酸層 4 過氧酸根層 5 丙烯酸及異丙基丙烯醯胺層 M274953 表1 · 溫度 15 25 41 53 (°C ) 不織布 1.000 士 0.001 1.000 士 0.096 1.000 士 0.004 1·〇〇〇 士 0·009 AN 10a 0.036士0.004 0.01 7土0.009 0.111土0.014 0.1 51士0.035 AN31b 0.039士0.006 0.114士0.035 0·160士0.012 0.269士0.055 AN11C 0.042士0.005 0.384士0.055 0.637士0.018 0.529土0.009 AN10Cd0_017±0.003 0.03士0.001 0.058士0.008 0.097士0.021 AN31Ce0.019：t0.002 0.05士0.001 0.062士0.008 0.109士0.009 ANllCf0.015±0_007 0_09士0.003 0.069士0.006 0.142士0.028 a·用丙烯酸/異丙基丙烯醯胺混合比例為1 /〇的混合溶液經 UV光照射所製備出的不織布 b·用丙烯酸/異丙基丙烯醯胺混合比例為3/1的混合溶液經 UV光照射所製備出的不織布 c·用丙烯酸/異丙基丙烯醯胺混合比例為1 /1的混合溶液經 UV光照射所製備出的不織布 · d· ANIO + chitosan經UV光照射所製備出的不織布 e· AN31+chitosan經UV光照射所製備出的不織布 f · AN11+chi tosan經UV光照射所製備出的不織布 M274953 表2· 樣本 綠膿桿菌 (CFU/ml) 金黃葡萄球菌 (CFU/ml) 空白 4.7χ 1011 3.〇χ ΙΟ10 不織布 8·8χ 10 丨1 2. 6 χ ΙΟ11 AN10 1·9χ 107 1.7 χ ΙΟ5 AN31 3.9χ ΙΟ7 2.0 χ ΙΟ5 AN11 1.5 χ ΙΟ8 1.1 χ ΙΟ6 AN10C 1·7χ ΙΟ6 9.〇χ ΙΟ4 AN31C 1.8 χ ΙΟ7 1.1 χ ΙΟ5 AN11C 3.1 χ ΙΟ7 1.6χ ΙΟ5 CFU = Colony Forming Units M274953 表3· 樣本 水氣透過速率 穿透性 透氣率 (WVTR) (Permeance) (Permeability) (g/m2/day) (Kg/ m2/Pa/s) (Kg/ m/Pa/s) ΧΙΟ8 xlO12 不織布 12869.000 4.750 4.040 ΑΝ10 18915.800 6.980 9.770 ΑΝ31 10200.500 3.760 8.660 ΑΝ11 3238.300 1.190 4.000 AN10C 4775.000 1.760 4.140 AN31C 6483.500 2.390 5.430 AN11C 4258.300 1.570 5.260 M274953 表4 . 樣本 ppa PP-aab PP-nipc PP-nipga-chiod PP-nipga-chiN€ 相對吸水時間 ^ !·〇〇0 ± 0.0500 0·85〇 ± 0.0600 °-44〇 ± 0.0600 °-〇〇l ± 0.0005 0.367 ± 0.2770 a ·不織布 b·經加馬射線所製備出的含丙烯酸不織布 C· ϊΐί射線、UV光照射、過氧酸化法所+ 丙烯醯胺不織布 7I備出的含異丙 d· ^加馬射線、UV光照射、過氧酸化法盥 出的含異丙基丙烯醯胺與聚甲殼醣不織、‘ d乾 e•經加馬射線、uv光照射、過氧酸化 出的含異丙基丙烯醯胺盘聚f之ίί;東乾燥法所製 f 、目丨4 〇來甲成醣不織布（於-1 9 6。(：冷讀 ^测4溫度25。(：. M274953 表5· 溫度（°c ) 15 25 38 53 PP 1.000 ±0.004 1.000 ±0.050 1.000 ±0.066 1.000 ±0.070 PP-nip 0.085 士 0.004 0.440 士 0.060 0.680 ± 0.026 0.686 ± 0.047 M274953 表6· 樣本 綠膿桿菌 金黃葡萄球菌 (CFU/ml) (CFU/ml) 空白 9.0 x 109 2.5 x 109 PP 1.3 x 108 1.0 x 109 PP-nip 1.0 x 108 2.1 x 107 PP-nipga-chio 3.7 x 104 2.2 x 103 CFU^Colony Forming Units M274953 表7· 樣本 水氣透過速 率（WVTR) (g/m2/day) 穿透性 (Permeance) (Kg/ m2/Pa/s) xlO9 透氣率 (Permeability) (Kg/ m/Pa/s) xl〇12 ΡΡ 10054.5 27.3 4.1 PP-nip 1199.3 3.3 1.5 PP-nipga-chio 2592.7 7.0 35.4 PP-nipga-chiN 3098.8 8.4 33.6 M274953 表8· 時間 綠膿桿菌 金黃葡萄球菌 (hr) (CFU/ml) (CFU/ml) 9 0 0 24 0 0 31 0 0 47 0 0 55 0 0Fig. 6A 'is a dressing containing polycarpose containing pp_nipga chjN. Schematic diagram of 40 times magnification of an electron microscope (SEM) on the face; Fig. 6B' is a dressing with polycarpose containing pp_njpga_ch 丨A schematic diagram of a 150-fold magnification of the electron microscope (SEM) of the N section; and Figure 7 is a schematic diagram of the antibacterial photograph of the original creation. Table 1 is a test table of the relative water absorption time and temperature sensitivity of the polysaccharides containing dressings of this creation; Table 2 is a table of the sterilization test results of the polysaccharides containing dressings of this creation; The water vapor transmission test table of the created chitosan-containing dressing; Table 4 'is the relative water absorption schedule of the created chitosan-containing dressing; Table 5' is the creation of the chitosan-containing dressing Temperature Sensitivity Test 17 Table M274953; Table 6 'It is a result of the sterilization test results of another creation containing a polysaccharide containing vinegar; Table 7 is the water vapor transmission of another creation containing a polysaccharide containing vinegar Test Table; and Bacterial Barrier Capacity Table 8 'This is the original test table containing isopropylacrylamide non-woven fabric. M274953 [Description of main component symbols] Substrate 1 Acrylic and isopropylacrylamide layer Polychitosan layer 3 Acrylic acid layer 4 Peroxyacid layer 5 Acrylic and isopropylacrylamide layer M274953 Table 1 · Temperature 15 25 41 53 (° C) Non-woven 1.000 ± 0.001 1.000 ± 0.096 1.000 ± 0.004 1 · 0.0000 ± 0 · 009 AN 10a 0.036 ± 0.004 0.01 7 ± 0.009 0.111 ± 0.014 0.1 51 ± 0.035 AN31b 0.039 ± 0.006 0.114 ± 0.035 0 · 160 ± 0.012 0.269 ± 0.055 AN11C 0.042 ± 0.005 0.384 ± 0.055 0.637 ± 0.018 0.529 ± 0.009 AN10Cd0_017 ± 0.003 0.03 ± 0.001 0.058 ± 0.008 0.097 ± 0.021 AN31Ce0.019: t0.002 0.05 ± 0.001 0.062 ± 0.008 0.109 ± 0.009 ANllCf0.015 ± 0_007 0_09 ± 0.003 0.069 ± 0.006 0.142 ± 0.028 a · Non-woven fabric prepared by irradiating UV light with a mixed solution of acrylic acid / isopropylacrylamide at a ratio of 1 / b b · Acrylic acid / isopropylacrylic acid Non-woven fabrics prepared by mixing a mixed solution with an amine mixing ratio of 3/1 under UV light c. Non-woven fabrics prepared by mixing a mixed solution with a mixing ratio of acrylic acid / isopropylacrylamide at 1/1 under UV light · D · ANIO + chitosan non-woven fabric prepared by UV light e · AN31 + chitosan non-woven fabric prepared by UV light f · AN11 + chi tosan non-woven fabric prepared by UV light M274953 Table 2 · Sample green Pseudomonas (CFU / ml) Staphylococcus aureus (CFU / ml) Blank 4.7χ 1011 3.〇χ ΙΟ10 Non-woven cloth 8 · 8χ 10 丨 1 2. 6 χ ΙΟ11 AN10 1 · 9χ 107 1.7 χ ΙΟ5 AN31 3.9χ ΙΟ7 2.0 χ ΙΟ5 AN11 1.5 χ ΙΟ8 1.1 χ ΙΟ6 AN10C 1 · 7χ ΙΟ6 9.〇χ ΙΟ4 AN31C 1.8 χ Ι07 1.1 χ ΙΟ5 AN11C 3.1 χ ΙΟ7 1.6χ ΙΟ5 CFU = Colony Forming Units M274953 Table 3. Rate (WVTR) (Permeance) (Permeability) (g / m2 / day) (Kg / m2 / Pa / s) (Kg / m / Pa / s) ΧΙΟ8 xlO12 Non-woven 12869.000 4.750 4.040 Α10 10915.800 6.980 9.770 ΑΝ31 10200.500 3.760 8.660 Α11 3238.300 1.190 4.000 AN10C 4775.000 1.760 4.140 AN31C 6483.500 2.390 5.430 AN11C 4258.300 1.570 5.260 M274953 Table 4. Sample ppa PP-aab PP-nipc PP-nipga-chiod PP-nipga-chiN € Relative water absorption time ^ ! · 〇〇0 ± 0.0500 0 · 85〇 ± 0.0600 ° -44〇 ± 0.0600 ° -〇〇l ± 0.0005 0.367 ± 0.2770 a · Non-woven fabric b · Acrylic-containing non-woven fabric C prepared by gamma rays · Isopropyl-containing d · ^ gamma rays prepared by UV light irradiation, peroxyacid method + acrylamide non-woven cloth 7I, isopropylacrylamide and polychitosan containing UV light, peroxyacid method Non-woven, 'd dry e • isopropylpropene amidamine disc polyf, produced by gamma rays, UV light irradiation, and peroxyacid; produced by Dong drying method, f. Non-woven (on -1 9 6). (: Cold reading ^ Measure 4 temperature 25. (: M274953 Table 5 · Temperature (° c) 15 25 38 53 PP 1.000 ± 0.004 1.000 ± 0.050 1.000 ± 0.066 1.000 ± 0.070 PP-nip 0.085 ± 0.004 0.440 ± 0.060 0.680 ± 0.026 0.686 ± 0.047 M274953 Table 6.Sample Pseudomonas aeruginosa (CFU / ml) (CFU / ml) Blank 9.0 x 109 2.5 x 109 PP 1.3 x 108 1.0 x 109 PP-nip 1.0 x 108 2.1 x 107 PP- nipga-chio 3.7 x 104 2.2 x 103 CFU ^ Colony Forming Units M274953 Table 7 · Sample water vapor transmission rate (WVTR) (g / m2 / day) Permeance (Kg / m2 / Pa / s) xlO9 breathable Permeability (Kg / m / Pa / s) x l〇12 PP 10054.5 27.3 4.1 PP-nip 1199.3 3.3 1.5 PP-nipga-chio 2592.7 7.0 35.4 PP-nipga-chiN 3098.8 8.4 33.6 M274953 Table 8. Time Pseudomonas aeruginosa Staphylococcus aureus (hr) (CFU / ml) (CFU / ml) 9 0 0 24 0 0 31 0 0 47 0 0 55 0 0