TWI834407B - Use of natural silicate platelet (nsp) in respiratory protection of livestock animals and method thereof - Google Patents
Use of natural silicate platelet (nsp) in respiratory protection of livestock animals and method thereof Download PDFInfo
- Publication number
- TWI834407B TWI834407B TW111146236A TW111146236A TWI834407B TW I834407 B TWI834407 B TW I834407B TW 111146236 A TW111146236 A TW 111146236A TW 111146236 A TW111146236 A TW 111146236A TW I834407 B TWI834407 B TW I834407B
- Authority
- TW
- Taiwan
- Prior art keywords
- nsp
- respiratory
- livestock
- poultry
- feed
- Prior art date
Links
- 244000144972 livestock Species 0.000 title claims abstract description 40
- 230000000241 respiratory effect Effects 0.000 title claims abstract description 27
- 241001465754 Metazoa Species 0.000 title abstract description 41
- 238000000034 method Methods 0.000 title abstract description 4
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 title abstract 2
- 230000014509 gene expression Effects 0.000 claims abstract description 14
- 108700042778 Antimicrobial Peptides Proteins 0.000 claims abstract description 4
- 102000044503 Antimicrobial Peptides Human genes 0.000 claims abstract description 4
- 230000028709 inflammatory response Effects 0.000 claims abstract description 4
- 244000144977 poultry Species 0.000 claims description 41
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 37
- 229910052710 silicon Inorganic materials 0.000 claims description 37
- 239000010703 silicon Substances 0.000 claims description 37
- 210000003437 trachea Anatomy 0.000 claims description 28
- 210000004072 lung Anatomy 0.000 claims description 15
- 210000002345 respiratory system Anatomy 0.000 claims description 15
- 239000003223 protective agent Substances 0.000 claims description 14
- 208000023504 respiratory system disease Diseases 0.000 claims description 11
- 239000003674 animal food additive Substances 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 5
- 150000002500 ions Chemical class 0.000 claims description 3
- 230000002554 disease preventive effect Effects 0.000 claims description 2
- 230000000704 physical effect Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 13
- 201000010099 disease Diseases 0.000 abstract description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 7
- 206010057190 Respiratory tract infections Diseases 0.000 abstract description 2
- 230000002401 inhibitory effect Effects 0.000 abstract description 2
- 239000011814 protection agent Substances 0.000 abstract 2
- 230000001737 promoting effect Effects 0.000 abstract 1
- 235000013594 poultry meat Nutrition 0.000 description 35
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 34
- 241000287828 Gallus gallus Species 0.000 description 27
- 229910021529 ammonia Inorganic materials 0.000 description 16
- 210000001519 tissue Anatomy 0.000 description 14
- 235000013330 chicken meat Nutrition 0.000 description 11
- 210000003608 fece Anatomy 0.000 description 10
- 210000002175 goblet cell Anatomy 0.000 description 9
- 108090000623 proteins and genes Proteins 0.000 description 9
- 235000018102 proteins Nutrition 0.000 description 8
- 102000004169 proteins and genes Human genes 0.000 description 8
- 238000011282 treatment Methods 0.000 description 8
- 241000271566 Aves Species 0.000 description 6
- 206010061218 Inflammation Diseases 0.000 description 6
- 102000003940 Occludin Human genes 0.000 description 6
- 108090000304 Occludin Proteins 0.000 description 6
- 208000015181 infectious disease Diseases 0.000 description 6
- 230000004054 inflammatory process Effects 0.000 description 6
- 102000007469 Actins Human genes 0.000 description 5
- 108010085238 Actins Proteins 0.000 description 5
- 102000004889 Interleukin-6 Human genes 0.000 description 5
- 108090001005 Interleukin-6 Proteins 0.000 description 5
- 230000000844 anti-bacterial effect Effects 0.000 description 5
- 230000037396 body weight Effects 0.000 description 5
- 229940100601 interleukin-6 Drugs 0.000 description 5
- 108020004999 messenger RNA Proteins 0.000 description 5
- KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 description 5
- 230000002829 reductive effect Effects 0.000 description 5
- 238000010186 staining Methods 0.000 description 5
- 208000005342 Porcine Reproductive and Respiratory Syndrome Diseases 0.000 description 4
- 210000000845 cartilage Anatomy 0.000 description 4
- 210000002919 epithelial cell Anatomy 0.000 description 4
- 230000008595 infiltration Effects 0.000 description 4
- 238000001764 infiltration Methods 0.000 description 4
- 230000002757 inflammatory effect Effects 0.000 description 4
- 210000000265 leukocyte Anatomy 0.000 description 4
- 210000004877 mucosa Anatomy 0.000 description 4
- 210000003097 mucus Anatomy 0.000 description 4
- 230000001717 pathogenic effect Effects 0.000 description 4
- 230000001681 protective effect Effects 0.000 description 4
- 235000019786 weight gain Nutrition 0.000 description 4
- 208000002979 Influenza in Birds Diseases 0.000 description 3
- 102000007296 Mucin-2 Human genes 0.000 description 3
- 108010008705 Mucin-2 Proteins 0.000 description 3
- 102000015728 Mucins Human genes 0.000 description 3
- 108010063954 Mucins Proteins 0.000 description 3
- 241000282898 Sus scrofa Species 0.000 description 3
- 102000000591 Tight Junction Proteins Human genes 0.000 description 3
- 108010002321 Tight Junction Proteins Proteins 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 206010064097 avian influenza Diseases 0.000 description 3
- 102000023732 binding proteins Human genes 0.000 description 3
- 108091008324 binding proteins Proteins 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 235000021050 feed intake Nutrition 0.000 description 3
- 230000002458 infectious effect Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000012188 paraffin wax Substances 0.000 description 3
- 230000008961 swelling Effects 0.000 description 3
- 230000036964 tight binding Effects 0.000 description 3
- 210000001578 tight junction Anatomy 0.000 description 3
- LOGFVTREOLYCPF-KXNHARMFSA-N (2s,3r)-2-[[(2r)-1-[(2s)-2,6-diaminohexanoyl]pyrrolidine-2-carbonyl]amino]-3-hydroxybutanoic acid Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@H]1CCCN1C(=O)[C@@H](N)CCCCN LOGFVTREOLYCPF-KXNHARMFSA-N 0.000 description 2
- 241000272517 Anseriformes Species 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 2
- 206010020751 Hypersensitivity Diseases 0.000 description 2
- 206010020880 Hypertrophy Diseases 0.000 description 2
- 102000003777 Interleukin-1 beta Human genes 0.000 description 2
- 108090000193 Interleukin-1 beta Proteins 0.000 description 2
- 241001673669 Porcine circovirus 2 Species 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 241000282887 Suidae Species 0.000 description 2
- 241000725681 Swine influenza virus Species 0.000 description 2
- 206010006451 bronchitis Diseases 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000032798 delamination Effects 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000007490 hematoxylin and eosin (H&E) staining Methods 0.000 description 2
- 210000002865 immune cell Anatomy 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 210000001533 respiratory mucosa Anatomy 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 229960005486 vaccine Drugs 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 101710128127 Beta-defensin 108B Proteins 0.000 description 1
- 101710125303 Beta-defensin 8 Proteins 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000282832 Camelidae Species 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 101710115621 Cathelicidin-3 Proteins 0.000 description 1
- 208000006545 Chronic Obstructive Pulmonary Disease Diseases 0.000 description 1
- 208000014085 Chronic respiratory disease Diseases 0.000 description 1
- 241000272201 Columbiformes Species 0.000 description 1
- 201000003883 Cystic fibrosis Diseases 0.000 description 1
- 101710178505 Defensin-1 Proteins 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 208000000666 Fowlpox Diseases 0.000 description 1
- 102000015696 Interleukins Human genes 0.000 description 1
- 108010063738 Interleukins Proteins 0.000 description 1
- 208000010359 Newcastle Disease Diseases 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000286209 Phasianidae Species 0.000 description 1
- 241000287502 Phoenicopteriformes Species 0.000 description 1
- 102100033214 Putative beta-defensin 108A Human genes 0.000 description 1
- 238000011529 RT qPCR Methods 0.000 description 1
- 206010068956 Respiratory tract inflammation Diseases 0.000 description 1
- 206010044302 Tracheitis Diseases 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 229910052784 alkaline earth metal Inorganic materials 0.000 description 1
- -1 alkaline earth metal cations Chemical class 0.000 description 1
- 239000013566 allergen Substances 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000003097 anti-respiratory effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 210000003555 cloaca Anatomy 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 235000021051 daily weight gain Nutrition 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical compound O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 description 1
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 201000009837 laryngotracheitis Diseases 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052901 montmorillonite Inorganic materials 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 239000002135 nanosheet Substances 0.000 description 1
- 210000001331 nose Anatomy 0.000 description 1
- 238000012758 nuclear staining Methods 0.000 description 1
- 235000019645 odor Nutrition 0.000 description 1
- 229920000620 organic polymer Polymers 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000006461 physiological response Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 206010039083 rhinitis Diseases 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 210000005092 tracheal tissue Anatomy 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
- 230000004584 weight gain Effects 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/70—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in livestock or poultry
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Description
本發明係關於一種利用天然矽片(NSP)做為保護禽畜類動物呼吸道之飼料添加劑的領域。更特別地，係關於天然矽片(NSP)作為飼料添加劑以改善禽畜類動物呼吸道組織形態、保護黏膜及抑制發炎反應的應用。 The present invention relates to the field of utilizing natural silicon flakes (NSP) as a feed additive for protecting the respiratory tract of livestock and poultry animals. More specifically, it relates to the application of natural silicon flakes (NSP) as feed additives to improve the respiratory tissue morphology of livestock and poultry animals, protect mucous membranes and inhibit inflammatory reactions.
禽畜類的呼吸道疾病已然成為畜產業最常見的疾病，而且其發病原因較為複雜，至今已發展成為一系列疾病的症候群。如禽畜類的呼吸道疾病包括傳染性喉氣管炎、傳染性支氣管炎、雞慢性呼吸道病、傳染性鼻炎、雞新城疫、禽流感、雞痘以及禽麴黴菌病等。而目前普遍流行於豬場中的有豬生殖和呼吸綜合症(PRRS)、第二型豬環狀病毒(PCV-2)與豬流行性感冒病毒(SIV)感染等；近幾年又形成以多病原因子所造成豬呼吸道疾病複合症(PRDC)成為豬場疾病流行的主流。禽畜類呼吸道性疾病依病原的特性、毒力或感染型式，可能造成家禽程度不等之症狀以及死亡，在經濟損失方面則包括飼料效率降低、日增重降低、屠體廢棄率增加、禽類產蛋率下降、蛋的品質變差以及孵化率降低等，重則引發多重疾病、死亡率增加，造成嚴重的經濟損失。 Respiratory diseases of poultry and livestock have become the most common diseases in the livestock industry, and their causes are complex and have developed into a series of disease syndromes. For example, respiratory diseases of poultry and livestock include infectious laryngotracheitis, infectious bronchitis, chronic respiratory disease of chickens, infectious rhinitis, Newcastle disease, avian influenza, fowl pox and avian zoomycosis. At present, porcine reproductive and respiratory syndrome (PRRS), porcine circovirus type 2 (PCV-2) and swine influenza virus (SIV) infections are commonly prevalent in pig farms; in recent years, there have been Porcine respiratory disease complex (PRDC) caused by multiple pathogenic factors has become the mainstream disease epidemic in pig farms. Depending on the characteristics, virulence or infection pattern of the pathogen, respiratory diseases in poultry and livestock may cause varying degrees of symptoms and death in poultry. In terms of economic losses, they include reduced feed efficiency, reduced daily weight gain, increased carcass waste rate, and poultry waste. Decreased egg production rate, poor egg quality, and reduced hatchability can lead to multiple diseases, increased mortality, and serious economic losses.
在禽畜類呼吸道疾病的防治方面，一般是採加強各方面的飼養管理、做好消毒防疫工作、適當的疫苗接種以及施予藥物例如抗生素防治，以期能有效的控制禽畜類呼吸道疾病的發生。然而，例如最顯著影響秋冬開始發病的高病原性禽流感(Avian Influenza)是不易以固定的毒株做為疫苗，再者如豬繁殖和呼 吸障礙綜合症(Porcine Reproductive and Respiratory Syndrome，PRRS)，因台灣不同的病毒株，而國外疫苗專一性不足，於本土豬隻施用結果功效有限。而且禽畜類呼吸道疾病的病原複雜、症狀表現不一，在診斷與治療上皆不易進行，因此加強禽畜類呼吸道的保護作用是畜產業重要的課題。 In terms of prevention and control of respiratory diseases in poultry and livestock, we generally adopt measures to strengthen all aspects of feeding and management, carry out disinfection and epidemic prevention work, provide appropriate vaccinations, and administer drugs such as antibiotics, in order to effectively control the occurrence of respiratory diseases in poultry and livestock. . However, for example, highly pathogenic avian influenza (Avian Influenza), which has the most significant impact on the onset of disease in autumn and winter, is difficult to use as a vaccine with a fixed strain. Moreover, such as pig reproduction and respiratory Porcine Reproductive and Respiratory Syndrome (PRRS), due to different virus strains in Taiwan and the lack of specificity of foreign vaccines, the results are limited when applied to local pigs. Moreover, the pathogens of respiratory diseases in poultry and livestock are complex and the symptoms vary, making diagnosis and treatment difficult. Therefore, strengthening the protective effect of the respiratory tract in poultry and livestock is an important issue for the livestock industry.
天然矽片(NSP)是一種奈米級的黏土脫層產品，NSP具有極高的裸露(naked)表面積(達約750m2/g)，以及陰離子性(至少18,000個離子每片)，讓其具有高效性吸附細菌、病毒、毒素、臭味等多重功能。利用NSP的構造可增加物理包被病原細胞的外表，達到干擾病毒接近感受細胞受體，以及包被細菌阻止增殖為其作用機制，且業經實驗證明其使用不會有抗藥性菌株之產生及食品安全衛生問題。 Natural Silicon Plate (NSP) is a nanoscale clay delamination product. NSP has an extremely high naked surface area (up to about 750m 2 /g) and anionic properties (at least 18,000 ions per piece), making it It has multiple functions such as efficiently absorbing bacteria, viruses, toxins, odors, etc. The structure of NSP can be used to increase the appearance of physically coated pathogenic cells, thereby interfering with the approach of viruses to receptors on sensing cells, and coating bacteria to prevent their proliferation as its mechanism of action, and it has been experimentally proven that its use will not cause the generation of drug-resistant strains and food Safety and health issues.
於是，本發明之一方面，係關於利用天然矽片(NSP)製備一種禽畜類呼吸道保護劑之用途。於本發明之一些具體實施例，所述之禽畜類呼吸道保護劑係用以防禦因呼吸道感染引起的疾病。 Therefore, one aspect of the present invention relates to the use of natural silicon flakes (NSP) to prepare a respiratory protective agent for poultry and livestock. In some embodiments of the present invention, the respiratory protective agent for poultry and livestock is used to prevent diseases caused by respiratory infections.
於本發明之一些具體實施例，所述之禽畜類呼吸道保護劑可為一種飼料添加劑。於本發明之一些具體實施例，所述之禽畜類呼吸道保護劑可為一種呼吸疾病預防性組合物。 In some specific embodiments of the present invention, the respiratory protective agent for poultry and livestock can be a feed additive. In some embodiments of the present invention, the respiratory protective agent for poultry and livestock may be a respiratory disease preventive composition.
於本發明之一具體實施例，所述禽畜類呼吸道保護劑係用以改善氣管完整性。於本發明之一具體實施例，所述禽畜類呼吸道保護劑係用以促進氣管與肺臟抗菌肽表達。於本發明之一具體實施例，所述禽畜類呼吸道保護劑係用以抑制呼吸道發炎反應。 In a specific embodiment of the present invention, the respiratory protective agent for poultry and livestock is used to improve the integrity of the trachea. In a specific embodiment of the present invention, the respiratory protective agent for poultry and livestock is used to promote the expression of antimicrobial peptides in the trachea and lungs. In a specific embodiment of the present invention, the poultry and livestock respiratory tract protective agent is used to inhibit respiratory tract inflammatory response.
於另一方面，本發明係關於一種用於提升禽畜類呼吸道保護力之飼料添加劑，特徵在於包含天然矽片(NSP)。較佳地，所述之飼料添加劑係以100-1000 ppm，較佳為500-1000ppm之量添加於基礎飼料中。 On the other hand, the present invention relates to a feed additive for improving respiratory protection of poultry and livestock, characterized by containing natural silicon flakes (NSP). Preferably, the feed additive is 100-1000 ppm, preferably 500-1000ppm added to the basic feed.
於另一方面，本發明係關於一種禽畜類呼吸疾病預防性飼料組合物，特徵在於包含用於提升禽畜類呼吸道保護力之天然矽片(NSP)。於本發明之一具體實施例，所述之飼料組合物包含100-1000ppm，較佳為500-1000ppm之天然矽片(NSP)。 On the other hand, the present invention relates to a preventive feed composition for respiratory diseases in poultry and livestock, which is characterized by including natural silicon flakes (NSP) for improving the protective power of the respiratory tract of poultry and livestock. In a specific embodiment of the present invention, the feed composition contains 100-1000 ppm, preferably 500-1000 ppm natural silicon flakes (NSP).
圖1a是顯示於飼料中添加天然矽片對於肉雞***物中氨含量的影響。圖1b是顯示於飼料中添加天然矽片對於肉雞***物中氨氣釋放量的影響。在肉雞第14、21、28和35日齡收集***物並進行氨氣分析。數值代表平均值±SE。條上的a,b；不同字母表示具有顯著差異(P<0.05)。*；表示相對於對照組具有顯著差異(P<0.05)。 Figure 1a shows the effect of adding natural silicon flakes to feed on ammonia content in broiler excreta. Figure 1b shows the effect of adding natural silicon flakes to feed on the amount of ammonia released in broiler chicken excreta. Feces were collected from broiler chickens at 14, 21, 28 and 35 days of age and analyzed for ammonia. Values represent mean ± SE. a,b on the bar; different letters indicate significant differences (P<0.05). *; indicates significant difference compared with the control group (P<0.05).
圖2為使用蘇木精和伊紅(H&E)染色CON組動物(A)與於飼料中添加天然矽片之NSP組動物(B)氣管切片的組織染色結果。軟骨厚度的結果代表氣於管周圍每45°角處的8次平均測量值。數值代表平均值±SE。條上的a,b；不同字母表示具有顯著差異(P<0.05)。放大倍率為4x。 Figure 2 shows the tissue staining results of tracheal sections of animals in the CON group (A) using hematoxylin and eosin (H&E) and the animals in the NSP group (B) with natural silicon flakes added to the feed. Cartilage thickness results represent the average of 8 measurements taken at every 45° angle around the tube. Values represent mean ± SE. a,b on the bars; different letters indicate significant differences (P<0.05). Magnification is 4x.
圖3為使用蘇木精和伊紅(H&E)染色CON組動物(A)與NSP組動物(B)氣管切片的組織染色結果。以整個氣管中段計數白細胞。通過切片的白細胞計數(具有細胞核染色的細胞，箭頭)量化白細胞浸潤。數值代表平均值±SE。條上的a,b；不同字母表示具有顯著差異(P<0.05)。放大倍率為20x。 Figure 3 shows the tissue staining results of tracheal sections of animals in the CON group (A) and NSP group (B) using hematoxylin and eosin (H&E) staining. White blood cells were counted throughout the mid-trachea. Leukocyte infiltration was quantified by leukocyte count of sections (cells with nuclear staining, arrows). Values represent mean ± SE. a,b on the bars; different letters indicate significant differences (P<0.05). Magnification is 20x.
圖4係顯示使用過碘酸(PA)染色CON組動物(A)與於飼料中添加天然矽片之NSP組動物(B)的黏膜厚度。結果代表氣於管周圍每45°角處的8次平均測量值。數值代表平均值±SE。條上的a,b；不同字母表示具有顯著差異(P<0.05)。箭頭所指為粘膜層。放大倍率為20x。 Figure 4 shows the mucosal thickness of animals in the CON group (A) stained with periodic acid (PA) and in the NSP group (B) with natural silicon flakes added to the feed. The results represent the average of 8 measurements taken at every 45° angle around the tube. Values represent mean ± SE. a,b on the bars; different letters indicate significant differences (P<0.05). The arrow points to the mucosal layer. Magnification is 20x.
圖5係顯示CON組動物(A)與於飼料中添加天然矽片之NSP組動物(B)的 過碘酸(PA)染色結果。以整個氣管中段計數杯狀細胞。數值代表平均值±SE。條上的a,b；不同字母表示具有顯著差異(P<0.05)。箭頭所指為杯狀細胞。放大倍率為20x。 Figure 5 shows the periodic acid (PA) staining results of animals in the CON group (A) and animals in the NSP group (B) whose feed was supplemented with natural silicon flakes. Goblet cells were counted throughout the midtrachea. Values represent mean ± SE. a,b on the bars; different letters indicate significant differences (P<0.05). The arrow points to the goblet cells. Magnification is 20x.
圖6係顯示於飼料中添加天然矽片對於肉雞氣管與肺臟組織中緊密結合蛋白(occludin，OCLN)mRNA表現的影響。以每組3個重複中的4個樣本進行分析。結果係對於β-肌動蛋白正常化，並以相對於對照組的比例表示。CON=對照組，NSP=於飼料中添加天然矽片之NSP處理組。數值代表平均值±SE。條上的a,b；不同字母表示具有顯著差異，P<0.05。 Figure 6 shows the effect of adding natural silicon flakes to feed on the expression of tight binding protein (occludin, OCLN) mRNA in broiler trachea and lung tissue. Analyzes were performed on 4 samples per set of 3 replicates. Results are normalized to β-actin and expressed as proportions relative to controls. CON=control group, NSP=NSP treatment group with natural silicon flakes added to the feed. Values represent mean ± SE. a,b on the bars; different letters indicate significant differences, P<0.05.
圖7係顯示於飼料中添加天然矽片對於肉雞氣管與肺臟組織中黏液蛋白mucin 2(MCU2)mRNA表現的影響。以每組3個重複中的4個樣本進行分析。結果係對於β-肌動蛋白正常化，並以相對於對照組的比例表示。CON=對照組，NSP=於飼料中添加天然矽片之NSP處理組。數值代表平均值±SE。條上的a,b；不同字母表示具有顯著差異，P<0.05。 Figure 7 shows the effect of adding natural silicon flakes to feed on the expression of mucin 2 (MCU2) mRNA in broiler trachea and lung tissue. Analyzes were performed on 4 samples per set of 3 replicates. Results are normalized to β-actin and expressed as proportions relative to controls. CON=control group, NSP=NSP treatment group with natural silicon flakes added to the feed. Values represent mean ± SE. a,b on the bars; different letters indicate significant differences, P<0.05.
圖8係顯示於飼料中添加天然矽片對於肉雞氣管與肺臟組織中抗菌蛋白AvBD8(avian β-defensin 8)mRNA表現的影響。以每組3個重複中的4個樣本進行分析。結果係對於β-肌動蛋白正常化，並以相對於對照組的比例表示。CON=對照組，NSP=於飼料中添加天然矽片之NSP處理組。數值代表平均值±SE。條上的a,b；不同字母表示具有顯著差異，P<0.05。 Figure 8 shows the effect of adding natural silicon flakes to feed on the expression of antibacterial protein AvBD8 (avian β-defensin 8) mRNA in broiler trachea and lung tissue. Analyzes were performed on 4 samples per set of 3 replicates. Results are normalized to β-actin and expressed as proportions relative to controls. CON=control group, NSP=NSP treatment group with natural silicon flakes added to the feed. Values represent mean ± SE. a,b on the bars; different letters indicate significant differences, P<0.05.
圖9係顯示於飼料中添加天然矽片對於肉雞氣管與肺臟組織中IL-1β mRNA表現的影響。結果係對於β-肌動蛋白正常化，並以相對於對照組的比例表示。數值代表平均值±SE,n=4。條上的a,b；不同字母表示具有顯著差異(P<0.05)。CON=對照組，NSP=於飼料中添加天然矽片之NSP處理組。 Figure 9 shows the effect of adding natural silicon flakes to feed on the expression of IL-1β mRNA in broiler trachea and lung tissue. Results are normalized to β-actin and expressed as proportions relative to controls. Values represent mean ± SE, n = 4. a,b on the bars; different letters indicate significant differences (P<0.05). CON=control group, NSP=NSP treatment group with natural silicon flakes added to the feed.
圖10係顯示於飼料中添加天然矽片對於肉雞氣管與肺臟組織中IL-6 mRNA表現的影響。結果係對於β-肌動蛋白正常化，並以相對於對照組的比例表 示。數值代表平均值±SE,n=4。條上的a,b；不同字母表示具有顯著差異(P<0.05)。CON=對照組，NSP=於飼料中添加天然矽片之NSP處理組。 Figure 10 shows the effect of adding natural silicon flakes to feed on the expression of IL-6 mRNA in broiler trachea and lung tissue. Results are normalized to β-actin and expressed as proportions relative to controls. Values represent mean ± SE, n = 4. a,b on the bars; different letters indicate significant differences (P<0.05). CON=control group, NSP=NSP treatment group with natural silicon flakes added to the feed.
本發明係關於一種天然矽片(NSP)做為禽畜類動物呼吸道保護劑之用途。本發明亦提供一種包含天然矽片(NSP)做為禽畜類動物呼吸道保護用添加劑之飼料組合物。 The present invention relates to the use of natural silicon flakes (NSP) as a respiratory protective agent for livestock and poultry animals. The present invention also provides a feed composition containing natural silicon flakes (NSP) as an additive for protecting the respiratory tract of livestock and poultry animals.
本文中，術語“天然矽片(NSP)”係指，一種藉由使用一端胺-曼尼斯寡聚物做有機高分子脫層劑，將經澎潤之蒙脫土直接脫層，繼而以鹼金屬陽離子或鹼土金屬陽離子進行置換而得到的奈米矽片，具有雙層平板或二片組成一個單位的結構、平均片徑大小為約100×100×1nm3、表面積為約200~800m2/g、離子電荷密度為約5,000~20,000離子/片，水溶液之等電點(isoelectric point,IEP)出現在pH=6.4。 In this article, the term "Natural Silica Plate (NSP)" refers to a method that uses an amine-terminated Mannes oligomer as an organic polymer delamination agent to directly delaminate the moistened montmorillonite, and then uses an alkali to Silicon nanosheets obtained by replacing metal cations or alkaline earth metal cations have a structure of double-layered flat plates or two sheets forming one unit, with an average sheet diameter of about 100×100×1nm3 and a surface area of about 200~800m2/g. The ionic charge density is about 5,000~20,000 ions/piece, and the isoelectric point (IEP) of the aqueous solution appears at pH=6.4.
本文中，“禽畜類動物”係指，經人工豢養的鳥類(家禽)及經是人類飼養馴化並且可控制繁殖的動物(家畜)。家禽類包括(但不限定於)鴨、雞、鴿子、鵝以及火鳥、鵪鶉等。家畜類包括(但不限定於)豬、牛、羊、馬、駱駝、兔、貓、狗等。 In this article, "poultry animals" refers to artificially raised birds (poultry) and animals (livestock) that have been domesticated by humans and whose reproduction can be controlled. Poultry includes (but is not limited to) ducks, chickens, pigeons, geese, flamingos, quail, etc. Livestock include (but are not limited to) pigs, cattle, sheep, horses, camels, rabbits, cats, dogs, etc.
本文中，“呼吸道保護”係指，利用物質或治療對動物鼻、氣管與肺臟產生保護作用。此類保護作用包括，改善禽畜類動物呼吸道組織形態、保護呼吸道黏膜及抑制發炎反應，其中發炎反應是指具有血管系統的活體組織對致炎因子及局部損傷所發生的防禦性為主的反應。 In this article, "respiratory protection" refers to the use of substances or treatments to protect the nose, trachea and lungs of animals. Such protective effects include improving the tissue morphology of the respiratory tract of livestock and poultry animals, protecting the respiratory mucosa, and inhibiting inflammatory reactions. Inflammatory reactions refer to the defensive response of living tissues with vascular systems to inflammatory factors and local damage. .
本發明之其他特色及優點將於下列實施範例中被進一步舉例與說明，而該實施範例僅作為輔助說明，並非用於限制本發明之範圍。 Other features and advantages of the present invention will be further exemplified and described in the following implementation examples, which are only used as auxiliary illustrations and are not intended to limit the scope of the present invention.
以下實施例使用之實驗動物為一日齡雄性白肉雞，購自台一農牧場(台 中，台灣)。依照體重平均分配至各處理組，進行為期5週的試驗。 The experimental animals used in the following examples were one-day-old male white broiler chickens purchased from Taiyi Farm (Taiwan). (China, Taiwan). The animals were evenly distributed to each treatment group according to body weight, and a 5-week experiment was conducted.
實施例一、於飼料中添加天然矽片(NSP)改善禽畜類動物呼吸道組織形態 Example 1. Adding natural silicon flakes (NSP) to feed to improve the respiratory tract tissue morphology of livestock and poultry animals
提高增重與採食 Improve weight gain and feed intake
將白肉雞分成2組，每組4重複(欄)，每欄5隻雞，分別為：對照組(Control,CON)，餵予基礎飼糧，無添加天然矽片(NSP)；於飼料中添加天然矽片之NSP組，餵予(基礎飼糧+500ppm NSP)。自實驗第0天開始，以每週為單位秤量體重，每日於固定時間點紀錄飼料採食量，並計算體增重及飼料轉換率。結果列示於下表1。 The white broiler chickens were divided into 2 groups, with 4 replicates (columns) in each group and 5 chickens in each column, respectively: control group (Control, CON), which was fed basic diet without adding natural silicon flakes (NSP); The NSP group of natural silicon flakes was fed (basic diet + 500ppm NSP). Starting from the 0th day of the experiment, the body weight was measured on a weekly basis, the feed intake was recorded at fixed time points every day, and the body weight gain and feed conversion rate were calculated. The results are shown in Table 1 below.
由表1之結果顯示，於飼料添加天然矽片(NSP)能提高雞隻的體增重與採食效率。 The results in Table 1 show that adding natural silicon flakes (NSP) to feed can improve the body weight gain and feed intake efficiency of chickens.
降低***物中氨含量及氨氣釋放 Reduce ammonia content and ammonia gas release in excrement
由於動物***出的氨氣(ammonia)會傷害禽類呼吸道絨毛，而絨毛受損亦會妨害動物生長，故若能降低***物中氨的含量，不僅可防止呼吸道絨毛受損傷，也有助於禽類生長。因此，於本實例中進一步測試，飼料添加天然矽片(NSP)對於肉雞***物中氨含量及氨氣釋放的影響。於14、21、28、35日齡，每組逢機取8隻作為樣本(每欄2隻)，以塑膠袋固定於雞隻泄殖腔，收集排出糞便4小時，隨即將糞便密封於15mL離心管中。將1gram分裝於另一離心管中，以石蠟膜 (parafilm)封口使其密封，低速離心使其完全沉澱於底部，置於28℃下30分鐘後，以橡膠導管伸入離心管中，橡膠導管另一頭再接氨氣測定儀，於1分鐘內測量氨氣濃度，取最大值。另以市售套組，分析糞便中的氨含量。 Since ammonia excreted by animals can damage the respiratory villi of poultry, and damaged villi will also hinder the growth of animals, if the ammonia content in excreta can be reduced, it will not only prevent damage to the respiratory villi, but also help the growth of poultry. . Therefore, in this example, the effect of adding natural silicon flakes (NSP) to feed on the ammonia content and ammonia release in broiler chicken excreta was further tested. At the age of 14, 21, 28, and 35 days, 8 birds from each group were taken as samples (2 birds from each column), fixed in the cloaca of the chickens with plastic bags, and the feces were collected for 4 hours, and then the feces were sealed in 15 mL centrifuge tubes. middle. Dispense 1gram into another centrifuge tube and cover with paraffin film Seal (parafilm) to seal, centrifuge at low speed to completely settle at the bottom, place it at 28°C for 30 minutes, insert a rubber tube into the centrifuge tube, and connect the other end of the rubber tube to an ammonia gas analyzer. Within 1 minute Measure the ammonia concentration and take the maximum value. A commercially available kit was also used to analyze the ammonia content in feces.
由圖1a的結果，在各時間點，於飼料中添加NSP的肉雞組皆顯示，添加NSP能明顯降低肉雞***物中之氨含量；另由圖1b的結果亦顯示，於飼料中添加NSP飼養雞隻，在肉雞25與35天日齡時，皆能降低糞便中氨氣釋放。表示飼料添加天然矽片(NSP)能有效減少肉雞的氨氣排放，可藉此改善造成禽類呼吸道絨毛損傷的有害環境，以達到保護飼養動物呼吸道的功效。 According to the results in Figure 1a, at each time point, the broiler chicken groups that added NSP to their feed showed that adding NSP could significantly reduce the ammonia content in the excreta of broiler chickens. The results in Figure 1b also showed that adding NSP to their feed raised them In broilers, when broilers are 25 and 35 days old, the release of ammonia in feces can be reduced. It means that adding natural silicon flakes (NSP) to feed can effectively reduce ammonia emissions from broilers, thereby improving the harmful environment that causes damage to the respiratory villi of poultry, so as to protect the respiratory tract of farmed animals.
改善呼吸道組織形態 Improve respiratory tissue morphology
為直接證明飼料添加NSP對於呼吸道組織形態的改善效能，於28日齡採樣，每組逢機取4隻作為樣本，取氣管(Trachea)做成石蠟切片，以H&E染色觀察組織形態，取氣管中段(middle line)測量軟骨層厚度(cartilage thickness)、黏膜層完整性(integrity)、與免疫細胞浸潤(leukocyte infiltration)。 In order to directly prove the effect of adding NSP in feed on improving the morphology of respiratory tract tissue, samples were taken at 28 days of age. Four animals were taken from each group as samples. The trachea (Trachea) were taken and made into paraffin sections. The tissue morphology was observed with H&E staining. The middle section of the trachea was taken. (Middle line) Measure cartilage thickness, mucosal layer integrity, and immune cell infiltration (leukocyte infiltration).
圖2之結果顯示，於飼料添加NSP能夠刺激餵食動物的氣管軟骨增生，並且提高氣管軟骨層厚度。而從圖3之結果顯示，於飼料添加NSP可降低免疫細胞浸潤，使氣管有較緻密與完整的結構，且呈現的上皮細胞整齊排列。相對於無NSP添加的對照組，NSP組動物的氣管黏膜層緻密完整，結構整齊排列，上皮細胞無腫脹，杯狀細胞也無增大(黑色箭頭所示)。 The results in Figure 2 show that adding NSP to feed can stimulate the proliferation of tracheal cartilage in fed animals and increase the thickness of the tracheal cartilage layer. The results in Figure 3 show that adding NSP to the feed can reduce immune cell infiltration, make the trachea have a denser and more complete structure, and the epithelial cells are neatly arranged. Compared with the control group without NSP addition, the tracheal mucosal layer of animals in the NSP group was dense and complete, with neatly arranged structures, no swelling of epithelial cells, and no enlargement of goblet cells (shown by black arrows).
另進一步確認添加NSP飼養動物對於改善氣管黏膜型態的影響。於28日齡採樣，每組逢機取4隻作為樣本，取氣管(Trachea)做成石蠟切片，以過碘酸希夫(Periodic acid Schiff，PAS)染色法，取氣管中段測量黏液分泌(mucin secretion)、黏膜層厚度(mucosal layer thickness)杯狀細胞數目(goblet cell number)。 It was also further confirmed that the effect of feeding animals with NSP on improving the morphology of tracheal mucosa was further confirmed. Samples were taken at the age of 28 days. Four animals were taken from each group as samples at an opportunity. The trachea (Trachea) was taken and made into paraffin sections. Periodic acid Schiff (PAS) staining was used to stain the middle part of the trachea to measure mucus secretion (mucin). secretion), mucosal layer thickness (mucosal layer thickness) and goblet cell number (goblet cell number).
圖4之結果顯示，於飼料中添加NSP會促進餵養動物之氣管黏膜層厚度增加，而上皮細胞則無腫脹現象。另外，由圖5之杯狀細胞染色結果顯示，飼料 中添加NSP可顯著降低氣管杯狀細胞增生與肥大。杯狀細胞在受到過敏原的刺激或有炎症產生時會迅速增加，同時分泌大量黏液，這是應對感染或過敏而產生的生理反應。由圖4、5之結果，表示於飼料中添加NSP可增強對於動物呼吸道的保護，減低產生發炎或過敏反應。 The results in Figure 4 show that adding NSP to feed will increase the thickness of the tracheal mucosal layer of fed animals, but there will be no swelling of epithelial cells. In addition, the goblet cell staining results in Figure 5 show that feed Adding NSP can significantly reduce tracheal goblet cell proliferation and hypertrophy. Goblet cells increase rapidly when stimulated by allergens or inflammation occurs, and secrete large amounts of mucus at the same time. This is a physiological response to infection or allergies. The results in Figures 4 and 5 indicate that adding NSP to feed can enhance the protection of animal respiratory tracts and reduce inflammation or allergic reactions.
實施例二、天然矽片(NSP)保護禽畜類動物呼吸道黏膜Example 2: Natural silicon sheet (NSP) protects the respiratory mucosa of livestock and poultry animals
於28日齡採樣，每組逢機取4隻作為樣本，刮取氣管(Trachea)黏膜，以qRT-PCR方法分析緊密結合蛋白occludin(OCLN)、黏液蛋白Mucin 2、及抗菌蛋白AvBD1(avian β-defensin 1)、AvBD8與抗菌肽cathelicidin 3(CADLN3)的基因表現。 Samples were taken at the age of 28 days. Four animals from each group were taken as samples at the opportunity. The trachea mucosa was scraped and analyzed using qRT-PCR method for the tight binding protein occludin (OCLN), mucus protein Mucin 2, and antibacterial protein AvBD1 (avian β). -Gene expression of defensin 1), AvBD8 and antimicrobial peptide cathelicidin 3 (CADLN3).
圖6之結果顯示，於飼料添加NSP會促進提升飼養動物之氣管與肺臟中的緊密結合蛋白occludin含量。緊密結合蛋白(occludin)是一種膜整合蛋白，為組成緊密連接(tight junction,TJ)的主要蛋白質成分之一，並且在緊密連接細胞旁通透性屏障的調節中具有特殊作用。 The results in Figure 6 show that adding NSP to feed will increase the content of the tight binding protein occludin in the trachea and lungs of farmed animals. Occludin is a membrane integral protein that is one of the main protein components of the tight junction (TJ) and plays a special role in the regulation of the paracellular permeability barrier of the tight junction.
已知黏液蛋白會在呼吸道疾病，例如哮喘、支氣管炎、慢性阻塞性肺病、囊性纖維化等中過度表現，而由圖7顯示，於飼料添加NSP會降低飼養動物之氣管與肺臟黏液蛋白mucin 2含量。另外，由圖8之結果顯示，於飼料添加NSP可提高飼養動物之氣管與肺臟抗菌蛋白AvBD8的表現量。 Mucin is known to be overexpressed in respiratory diseases, such as asthma, bronchitis, chronic obstructive pulmonary disease, cystic fibrosis, etc. As shown in Figure 7, adding NSP to feed will reduce the mucin protein mucin in the trachea and lungs of farmed animals. 2 content. In addition, the results in Figure 8 show that adding NSP to feed can increase the expression of the antibacterial protein AvBD8 in the trachea and lungs of farmed animals.
綜合上述對於呼吸道黏膜蛋白表現的測試結果，於飼料添加NSP會提高飼養動物之氣管與肺臟抗菌蛋白AvBD8基因表現、降低黏液蛋白mucin 2表現，但對抗菌蛋白AvBD1與CATLN3的含量變化沒影響。 Based on the above test results on the performance of respiratory mucosal proteins, adding NSP to feed will increase the expression of the antibacterial protein AvBD8 gene in the trachea and lungs of farmed animals and reduce the expression of the mucus protein mucin 2, but has no effect on the changes in the content of the antibacterial proteins AvBD1 and CATLN3.
實施例三、天然矽片(NSP)抑制禽畜類動物呼吸道發炎反應Example 3: Natural silicon flakes (NSP) inhibit respiratory tract inflammatory response in livestock and poultry animals
於本實例係進一步檢視，飼料中添加NSP對於飼養動物抗呼吸道發炎的作用。於28日齡採樣，每組逢機取4隻作為樣本，刮取氣管(Trachea)黏膜，以西方吸漬法(Western blot)分析介白素Interleukin-1β(IL-1β)與介白素Interleukin-6(IL-6)的含量。 In this example, the effect of adding NSP to feed on anti-respiratory tract inflammation in farmed animals was further examined. Samples were taken at the age of 28 days. Four animals from each group were taken as samples at the opportunity. The trachea mucosa was scraped and analyzed for Interleukin-1β (IL-1β) and Interleukin by Western blot. -6 (IL-6) content.
圖9之結果顯示，以添加NSP之飼料餵養的雞隻(NSP組)，其氣管與肺臟中的介白素-1β(IL-1β)含量，明顯較無添加NSP飼料餵養的雞隻(對照組CON組)低，表示於飼料中添加NSP可降低飼養動物的氣管發炎因子IL-1β產生。此外，由圖10之結果亦顯示，以添加NSP之飼料餵養的雞隻(NSP組)，其氣管與肺臟中的介白素-6(IL-6)含量，明顯較無添加NSP飼料餵養的雞隻(對照組CON組)低，表示於飼料中添加NSP也會降低氣管與肺臟中的發炎因子IL-6產生。 The results in Figure 9 show that the interleukin-1β (IL-1β) content in the trachea and lungs of chickens fed with NSP-supplemented feed (NSP group) was significantly lower than that of chickens fed with no NSP-supplemented feed (control group). group CON), indicating that adding NSP to feed can reduce the production of the tracheal inflammatory factor IL-1β in animals. In addition, the results in Figure 10 also show that the interleukin-6 (IL-6) content in the trachea and lungs of chickens fed with NSP-added feed (NSP group) was significantly higher than that of chickens fed with no NSP-added feed. The chickens (control group CON group) were lower, indicating that adding NSP to the feed would also reduce the production of the inflammatory factor IL-6 in the trachea and lungs.
綜合以上的實驗結果，從氣管組織形態上顯示，添加NSP對於飼料餵養的動物的呼吸系統有正面作用：相較於CON組，於飼料中添加天然矽片之NSP組雞隻之氣管黏膜層緻密完整，結構整齊排列，上皮細胞無腫脹，且杯狀細胞無增生與肥大情形；再配合氣管中黏膜蛋白與發炎因子表現的數據，表示NSP可透過改善氣管完整性、促進抗菌肽表達和抑制發炎反應，進而對呼吸道產生有益的保護作用。此外，由***物氨氣含量的測定結果顯示，於飼料中添加天然矽片可有效降低***物中的氨含量及氨氣釋放，藉以改善飼養場環境中會對禽畜類呼吸道絨毛造成損傷的不利條件，並提高禽畜類動物的體增重與採食效率。 Based on the above experimental results, the tracheal tissue morphology shows that adding NSP has a positive effect on the respiratory system of feed-fed animals: compared with the CON group, the tracheal mucosa of the chickens in the NSP group with natural silicon flakes added to the feed is denser. Complete, the structure is neatly arranged, there is no swelling of epithelial cells, and there is no hyperplasia and hypertrophy of goblet cells; combined with the data on the expression of mucosal proteins and inflammatory factors in the trachea, it shows that NSP can improve the integrity of the trachea, promote the expression of antimicrobial peptides and inhibit inflammation reaction, thereby producing a beneficial protective effect on the respiratory tract. In addition, the measurement results of the ammonia content in excrement show that adding natural silicon flakes to feed can effectively reduce the ammonia content and ammonia release in excrement, thereby improving the environment of the feedlot that can cause damage to the respiratory villi of poultry and livestock. adverse conditions, and improve the body weight gain and feed efficiency of livestock and poultry animals.
雖然以有限數量的實施例說明本發明的實施方式，本領域具通常知識的技術人員仍有可能加以修改或變化，因此本發明的保護範圍應只受專利範圍限制，而非受限於上述實施例。 Although a limited number of examples are used to illustrate the implementation of the present invention, those skilled in the art may still make modifications or changes. Therefore, the protection scope of the present invention should only be limited by the scope of the patent and not by the above implementations. example.
Claims (6)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW111146236A TWI834407B (en) | 2022-12-01 | 2022-12-01 | Use of natural silicate platelet (nsp) in respiratory protection of livestock animals and method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW111146236A TWI834407B (en) | 2022-12-01 | 2022-12-01 | Use of natural silicate platelet (nsp) in respiratory protection of livestock animals and method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| TWI834407B true TWI834407B (en) | 2024-03-01 |
| TW202423292A TW202423292A (en) | 2024-06-16 |
Family
ID=91269493
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TW111146236A TWI834407B (en) | 2022-12-01 | 2022-12-01 | Use of natural silicate platelet (nsp) in respiratory protection of livestock animals and method thereof |
Country Status (1)
| Country | Link |
|---|---|
| TW (1) | TWI834407B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW201332441A (en) * | 2012-02-15 | 2013-08-16 | Nat Univ Chung Hsing | Avian growth promoting agent comprising nanosilicate plate |
| TW201334700A (en) * | 2012-02-22 | 2013-09-01 | Nat Univ Chung Hsing | Inhibition of mycotoxin with nano silicate platelet |
| CN103548874A (en) * | 2013-10-25 | 2014-02-05 | 龙口市海盛农牧有限公司 | Environment cleaning agent for cultivation |
| CN106359450A (en) * | 2016-08-23 | 2017-02-01 | 北京老哈默国际农业科技发展有限公司 | Livestock and poultry farm environment improver and preparation method thereof |
| CN107441325A (en) * | 2017-08-08 | 2017-12-08 | 上海朝翔生物技术有限公司 | A kind of natural galenical for promoting livestock and poultry respiratory health and preparation method thereof |
| TW202112669A (en) * | 2019-08-22 | 2021-04-01 | 多能顧問股份有限公司 | Metallic oxide/nsp nano-composite and method for producing the same |
-
2022
- 2022-12-01 TW TW111146236A patent/TWI834407B/en active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW201332441A (en) * | 2012-02-15 | 2013-08-16 | Nat Univ Chung Hsing | Avian growth promoting agent comprising nanosilicate plate |
| TW201334700A (en) * | 2012-02-22 | 2013-09-01 | Nat Univ Chung Hsing | Inhibition of mycotoxin with nano silicate platelet |
| CN103548874A (en) * | 2013-10-25 | 2014-02-05 | 龙口市海盛农牧有限公司 | Environment cleaning agent for cultivation |
| CN106359450A (en) * | 2016-08-23 | 2017-02-01 | 北京老哈默国际农业科技发展有限公司 | Livestock and poultry farm environment improver and preparation method thereof |
| CN107441325A (en) * | 2017-08-08 | 2017-12-08 | 上海朝翔生物技术有限公司 | A kind of natural galenical for promoting livestock and poultry respiratory health and preparation method thereof |
| TW202112669A (en) * | 2019-08-22 | 2021-04-01 | 多能顧問股份有限公司 | Metallic oxide/nsp nano-composite and method for producing the same |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2021254118A1 (en) | Applications of hpk1 kinase inhibitor in preventing and/or treating animal pathogen infection | |
| BR112015007549B1 (en) | Clay product and its uses | |
| JP2016053050A (en) | Improvement of gastrointestinal health, immunity, and performance by dietary intervention | |
| Zarnab et al. | Effects of Induced High Ammonia Concentration in Air on Gross and Histopathology of Different Body Organs in Experimental Broiler Birds and its Amelioration by Different Modifiers. | |
| KR101656791B1 (en) | Composition of feed additives having anti-virus activity | |
| JP7442662B2 (en) | Use of compounds in the prevention and/or treatment of pathogenic infections in animals | |
| TWI834407B (en) | Use of natural silicate platelet (nsp) in respiratory protection of livestock animals and method thereof | |
| JP5865350B2 (en) | Vaccine against Mycoplasma hyonyum monnier suitable for administration in the presence of maternally derived antibodies | |
| KR101929141B1 (en) | Antiviral effects of narasin in swine feed | |
| Bhanja et al. | In ovo nano-silver and nutrient supplementation improves immunity and resistance against Newcastle disease virus challenge in broiler chickens | |
| TW202423292A (en) | Use of natural silicate platelet (nsp) in respiratory protection of livestock animals and method thereof | |
| Hegazy et al. | An experimental co-infection of broilers with local isolates of Ornithobacterium rhinotracheale and Escherichia coli | |
| CN107158370B (en) | Raccoon dog canine distemper freeze-dried live vaccine and preparation method and application thereof | |
| Landman et al. | Investigations of Enterococcus faecalis-induced bacteraemia in brown layer pullets through different inoculation routes in relation to the production of arthritis | |
| Ellakany et al. | Studies on the effects of enrofloxacin overdose on different health parameters in broiler chickens | |
| Ko et al. | Immunomodulatory responses in plectasin-supplemented broilers under tropical environmental conditions | |
| CN107236715B (en) | Raccoon dog distemper virus attenuated vaccine strain and application thereof in preparation of raccoon dog distemper live vaccine | |
| CN111568861A (en) | Bromhexine hydrochloride oral liquid for livestock and poultry as well as preparation method and application thereof | |
| Compiani et al. | Comparison of tildipirosin and tulathromycin for control of bovine respiratory disease in high-risk beef heifers | |
| TWI727638B (en) | Use of fucoidan for inhibiting avian influenza virus | |
| Tamilmaran et al. | Occurrence and pathology of infectious laryngotracheitis (ILT) in commercial layer chicken | |
| Amer et al. | Field study on control of chronic respiratory disease in vertically infected broiler chicks | |
| CN117679362B (en) | Veterinary valnemulin solution and preparation method and application thereof | |
| Amer et al. | Experimentally induction and control of cellulitis in broiler chickens | |
| CN114748460B (en) | Medicine for treating calf cryptosporidium infection and application thereof |