TWI823559B - Discrete spectroscopic detection device - Google Patents
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Abstract
一種離散分光式檢測裝置，包含殼體、光源發射器、分光鏡、聚光鏡、濾光片、準直單元、離散式光感測單元及處理單元。殼體具有待測物容置槽供容置待測物。光源發射器產生檢測光線，並向分光鏡發射檢測光線。分光鏡反射檢測光線後通過聚光鏡，使檢測光線聚焦於待測物上，且待測物散射出拉曼散射光線。拉曼散射光線依序通過聚光鏡、分光鏡、濾光片及準直單元，由準直單元將拉曼散射光線準直成準直光線，離散式光感測單元接收並根據準直光線產生複數光強度感測訊號，供處理單元根據複數光強度感測訊號產生一檢測結果。通過離散分光式檢測裝置進行檢測，可快速確認待測物的檢測結果，無需前往實驗室對待測物進行分析，進而避免讓使用者攝取到危害身體的有毒物質，減少有毒物質累積在體內的風險。A discrete spectroscopic detection device includes a housing, a light source emitter, a spectroscope, a condenser mirror, a filter, a collimation unit, a discrete light sensing unit and a processing unit. The housing has an object receiving slot for accommodating the object to be tested. The light source emitter generates detection light and emits the detection light to the spectroscope. The detection light is reflected by the spectroscope and then passes through the condenser, so that the detection light is focused on the object to be measured, and the object to be measured scatters the Raman scattered light. The Raman scattered light passes through the condenser, beam splitter, filter and collimation unit in sequence. The collimation unit collimates the Raman scattered light into collimated light. The discrete light sensing unit receives and generates complex numbers based on the collimated light. The light intensity sensing signal is used by the processing unit to generate a detection result based on the plurality of light intensity sensing signals. Detection through discrete spectroscopic detection devices can quickly confirm the test results of the object to be tested, without the need to go to the laboratory to analyze the object to be tested, thus preventing users from ingesting toxic substances that are harmful to the body and reducing the risk of toxic substances accumulating in the body. .
Description
本發明係有關於一種檢測裝置，尤其是一種離散分光式檢測裝置。The present invention relates to a detection device, in particular to a discrete spectroscopic detection device.
孔雀石綠(malachite green)最早係運用在衣物、羊毛、紙張的染色上，之後被發現孔雀石綠水溶液可以減少真菌感染與殺死寄生蟲，預防魚類傷口感染，就開始大量運用在水產產業，如養殖或運輸消毒用。Malachite green was first used in the dyeing of clothing, wool, and paper. Later, it was discovered that malachite green aqueous solution can reduce fungal infections, kill parasites, and prevent fish wound infections. It began to be widely used in the aquatic industry. Such as breeding or transportation disinfection.
雖然孔雀石綠可以消除水生環境的細菌，但⾧時間使用發現孔雀石綠係為一種有毒物質，其不但會對魚蝦類健康產生影響，若人類攝取含有孔雀石綠的魚蝦累積過量，也會對健康造成重大的影響，例如孔雀石綠中的毒素會造成致癌、基因受損等危害健康的風險。Although malachite green can eliminate bacteria in the aquatic environment, over time it has been found that malachite green is a toxic substance. It not only affects the health of fish and shrimps, but also if humans ingest excessive amounts of fish and shrimp containing malachite green, it will also It will have a significant impact on health. For example, the toxins in malachite green can cause cancer, genetic damage and other health risks.
目前檢測有毒物質，例如孔雀石綠，的方式係通過液相層析儀／質譜儀(Liquid Chromatograph/Mass Spectrometer)進行檢測，而通過液相層析儀／質譜儀進行檢測需要將檢體送入液相層析儀進行分離純化，把檢體內複雜的成分，按時間一一將純化後的檢體送入質譜儀做進一步的檢測。但由於液相層析儀／質譜儀皆為昂貴的實驗設備，故只有在專業實驗室才能進行檢測。且液相層析儀／質譜儀需要經過特別訓練的人員才能操作。此外，通過液相層析儀／質譜儀進行檢測的檢測時間⾧，無法即時知道結果。The current method of detecting toxic substances, such as malachite green, is through liquid chromatograph/mass spectrometer (Liquid Chromatograph/Mass Spectrometer), and detection by liquid chromatograph/mass spectrometer requires the sample to be sent into The liquid chromatograph separates and purifies the complex components in the sample and sends the purified sample one by one to the mass spectrometer for further detection according to time. However, since liquid chromatography/mass spectrometers are expensive experimental equipment, testing can only be performed in professional laboratories. And liquid chromatography/mass spectrometers require specially trained personnel to operate. In addition, due to the detection time of the liquid chromatograph/mass spectrometer, the results cannot be known immediately.
另一種檢測檢測有毒物質的方式係通過快速檢測試片進行檢測。但檢測前，需要先使用萃取液對檢體溶液萃取40分鐘，再將萃取後的檢體溶液滴在快速檢測試片上反應15分鐘才能知道檢測結果，仍相當耗時。此外，萃取液與快速檢測試片都是耗材，對環境不友善，且使用是生化方法，導致消費者的使用意願較低。Another way to detect toxic substances is through rapid detection test strips. However, before testing, the sample solution needs to be extracted with an extraction solution for 40 minutes, and then the extracted sample solution is dropped on the rapid test strip to react for 15 minutes before the test result can be known, which is still quite time-consuming. In addition, the extraction solution and rapid test strips are consumables, which are not friendly to the environment, and they use biochemical methods, resulting in low consumer willingness to use them.
因此，目前檢測有毒物質的方式仍續進一步之改良。Therefore, current methods for detecting toxic substances continue to be further improved.
有鑑於上述問題，本發明提供一種離散分光式檢測裝置，藉由簡易式的拉曼光譜分析方法，檢測檢體中是否含有有毒物質的光譜訊號，故不需要到實驗室對檢體進行分析，且能快速確認檢測結果，進而避免攝取到危害身體的有毒物質，減少有毒物質累積在體內的風險。In view of the above problems, the present invention provides a discrete spectroscopic detection device that uses a simple Raman spectrum analysis method to detect whether the sample contains spectral signals of toxic substances. Therefore, there is no need to go to the laboratory to analyze the sample. And it can quickly confirm the test results, thereby avoiding the ingestion of toxic substances that harm the body and reducing the risk of toxic substances accumulating in the body.
本發明的離散分光式檢測裝置，包含一殼體、一光源發射器、一分光鏡、一聚光鏡、一濾光片、一準直單元、一離散式光感測單元及一處理單元。The discrete spectroscopic detection device of the present invention includes a housing, a light source emitter, a beam splitter, a condenser lens, a filter, a collimation unit, a discrete light sensing unit and a processing unit.
該殼體具有一待測物容置槽，供容置一待測物。該光源發射器設置於該殼體內，且產生一檢測光線。該檢測光線之頻率為一第一頻率。該分光鏡設置於該殼體內，且該光源發射器朝向該分光鏡發射該檢測光線。該聚光鏡設置於該殼體的該待測物容置槽的一槽側壁。該檢測光線經該分光鏡反射後，射向該聚光鏡，並通過該聚光鏡聚光於該待測物容置槽中容置的該待測物，以透過該待測物散射出一拉曼散射光線。該拉曼散射光線包含有複數頻率之光線，且該拉曼散射光線之該些頻率不同於該第一頻率。The housing has an object receiving slot for accommodating an object to be tested. The light source emitter is arranged in the housing and generates a detection light. The frequency of the detection light is a first frequency. The spectroscope is disposed in the housing, and the light source emitter emits the detection light toward the spectroscope. The condenser lens is arranged on a side wall of the object receiving groove of the housing. After the detection light is reflected by the spectroscope, it is directed to the condenser lens, and is focused on the object to be measured accommodated in the object to be measured through the condenser lens, so as to scatter a Raman scattering through the object to be measured. light. The Raman scattered light includes light with complex frequencies, and the frequencies of the Raman scattered light are different from the first frequency.
該濾光片設置於該殼體內。該拉曼散射光線通過該聚光鏡射回該分光鏡，並經該分光鏡折射後，射向該濾光片。該濾光片係濾除頻率為該第一頻率之光線。該準直單元設置於該殼體內。該拉曼散射光線通過該濾光片後，射向該準直單元，且經由該準直單元形成一準直光線。該離散式光感測單元設置於該殼體內。該準直光線自該準直單元射向該離散式光感測單元，且該離散式光感測單元根據該準直光線產生複數光強度感測訊號。該處理單元電連接該離散式光感測單元，並接收該些光強度感測訊號，且根據該些光強度感測訊號產生一檢測結果。The optical filter is arranged in the housing. The Raman scattered light is reflected back to the spectroscope through the condenser mirror, refracted by the spectroscope, and then directed to the filter. The optical filter filters out light with the frequency of the first frequency. The collimation unit is arranged in the housing. After the Raman scattered light passes through the filter, it is emitted to the collimating unit, and forms a collimated light through the collimating unit. The discrete light sensing unit is disposed in the housing. The collimated light rays are emitted from the collimating unit to the discrete light sensing unit, and the discrete light sensing unit generates complex light intensity sensing signals according to the collimated light rays. The processing unit is electrically connected to the discrete light sensing unit, receives the light intensity sensing signals, and generates a detection result based on the light intensity sensing signals.
一般而言，當一入射光照射到一待測樣品時，會發生散射。若散射出來的散射光的能量有發生改變，也就是散射光的頻率改變，此種狀況稱作非彈性碰撞，也稱作拉曼散射。Generally speaking, when an incident light hits a sample to be measured, scattering occurs. If the energy of the scattered light changes, that is, the frequency of the scattered light changes, this situation is called inelastic collision, also called Raman scattering.
詳細來說，拉曼散射是利用待測樣品的散射現象來測定晶格及分子的震動、旋轉模式。當入射光照射到待測樣品的分子時，入射光會和分子的分子鍵產生交互作用，而每個分子鍵都有其獨特的震動模式，故能夠藉由拉曼散射來量測分子震動的能量差，以鑑定內容物的種類。Specifically, Raman scattering uses the scattering phenomenon of the sample to be measured to measure the vibration and rotation modes of the crystal lattice and molecules. When incident light irradiates the molecules of the sample to be measured, the incident light will interact with the molecular bonds of the molecules. Each molecular bond has its own unique vibration mode, so the vibration of the molecules can be measured through Raman scattering. Energy difference to identify the type of content.
而本發明在使用時，只需將該待測物放置於該待測物容置槽中，即可通過該離散式光感測單元接收該待測物散射出的該拉曼散射光線，並根據該拉曼散射產生對應的該些光強度訊號，再由該處理單元根據該些光強度訊號產生該檢測結果。也就是說，本發明讓使用者無需前往實驗室對待測物進行分析，即可確認檢測結果，且本發明係通過光學方式檢測，能夠快速判斷待測物的檢測結果，進而避免讓使用者攝取到危害身體的有毒物質，減少有毒物質累積在體內的風險。同時，本發明能夠讓未受過特別訓練的一般使用者操作，也不容易產生耗材，故能提高使用者的使用意願。When the present invention is used, the object to be measured only needs to be placed in the object to be measured, and the Raman scattered light scattered by the object to be measured can be received through the discrete light sensing unit, and The corresponding light intensity signals are generated according to the Raman scattering, and then the processing unit generates the detection result based on the light intensity signals. In other words, the present invention allows users to confirm the test results without going to the laboratory to analyze the object to be tested. Moreover, the present invention detects through optical means, which can quickly determine the test results of the object to be tested, thereby preventing the user from ingesting the test object. Remove toxic substances that harm the body and reduce the risk of toxic substances accumulating in the body. At the same time, the present invention can be operated by ordinary users without special training and is not prone to producing consumables, so it can improve users' willingness to use it.
請參閱圖1、圖2及圖3所示，本發明的離散分光式檢測裝置包含有一殼體10、一處理單元20、一光源發射器30、一分光鏡40、一聚光鏡50、一濾光片60、一準直單元70及一離散式光感測單元80。Please refer to Figures 1, 2 and 3. The discrete spectroscopic detection device of the present invention includes a
該殼體10具有一待測物容置槽11，供容置一待測物111。該光源發射器30設置於該殼體10內，且產生一檢測光線。該檢測光線之頻率為一第一頻率。該分光鏡40設置於該殼體10內，且該光源發射器30朝向該分光鏡40發射該檢測光線。該聚光鏡50設置於該殼體10的該待測物容置槽11的一槽側壁。該檢測光線經該分光鏡40反射後，射向該聚光鏡50，並通過該聚光鏡50聚光於該待測物容置槽11中容置的該待測物111，以透過該待測物111散射出一拉曼散射光線。該拉曼散射光線包含有複數頻率之光線，且該拉曼散射光線之該些頻率不同於該第一頻率。The
該濾光片60設置於該殼體內。該拉曼散射光線通過該聚光鏡50射回該分光鏡40，並經該分光鏡40折射後，射向該濾光片60。該濾光片60係濾除頻率為該第一頻率之光線。該準直單元70設置於該殼體內。該拉曼散射光線通過該濾光片60後，射向該準直單元70，且經由該準直單元70形成一準直光線。該離散式光感測單元80設置於該殼體內。該準直光線自該準直單元70射向該離散式光感測單元80，且該離散式光感測單元80根據該準直光線產生複數光強度感測訊號。該處理單元20電連接該離散式光感測單元80，並接收該些光強度感測訊號，且根據該些光強度感測訊號產生一檢測結果。The
請參閱圖1及圖2所示，該離散分光式檢測裝置還包含有一啟動按鍵101、一顯示單元102及一電源按鍵103。Please refer to FIG. 1 and FIG. 2 . The discrete spectroscopic detection device also includes a
該啟動按鍵101、該顯示單元102及該電源按鍵103分別電連接該處理單元20。該電源按鍵103係用於啟動或關閉該離散分光式檢測裝置的電源。該顯示單元102係用於接收並顯示該檢測結果。該啟動按鍵101則係供使用者操作，用於觸發該離散分光式檢測裝置的檢測程序。舉例來說，當該啟動按鍵101被觸發時，該啟動按鍵101產生並傳送一開始檢測訊號至該處理單元20。而當該處理單元20接收到該開始檢測訊號時，該處理單元20啟動該光源發射器30，並接收該離散式光感測單元80產生的該些光強度感測訊號。且該處理單元20根據該些光強度感測訊號產生該檢測結果，並通過該顯示單元102顯示該檢測結果。The
該離散分光式檢測裝置在使用時，係由使用者將該待測物111放置於該待測物容置槽11中。接著，使用者可通過按壓該啟動按鍵103來觸發，以產生該開始檢測訊號。當該處理單元20接收到該開始檢測訊號時，該處理單元20即可啟動該光源發射器30，以發射該檢測光線。且該處理單元20還可通過該離散式光感測單元80接收該待測物111散射出的該拉曼散射光線，並根據該拉曼散射產生對應的該些光強度訊號。最後，再由該處理單元20根據該些光強度訊號產生該檢測結果。When the discrete spectroscopic detection device is used, the user places the
如此一來，使用者便無需前往實驗室對待測物進行分析，通過該離散分光式檢測裝置即可確認檢測結果。且該離散分光式檢測裝置係通過光學方式檢測，能夠快速判斷待測物的檢測結果，進而避免讓使用者攝取到危害身體的有毒物質，減少有毒物質累積在體內的風險。同時，該離散分光式檢測裝置能夠讓未受過特別訓練的一般使用者操作，也不容易產生耗材，故能提高使用者的使用意願。In this way, users do not need to go to the laboratory to analyze the analyte, and can confirm the test results through the discrete spectroscopic detection device. Moreover, the discrete spectroscopic detection device detects through optical methods, which can quickly determine the detection results of the object to be tested, thus preventing users from ingesting toxic substances that are harmful to the body and reducing the risk of toxic substances accumulating in the body. At the same time, the discrete spectroscopic detection device can be operated by ordinary users without special training, and it is not easy to produce consumables, so it can increase users' willingness to use it.
請參閱圖3所示，在本實施例中，該光源發射器30係一雷射發射器，且該雷射發射器發射的該檢測光束的波長介於532奈米(nm)~1064奈米之間。該濾光片60係一帶阻濾光片或一低通濾光片。該檢測光線入射該分光鏡40的一入射角θ為45度。Please refer to Figure 3. In this embodiment, the
該光源發射器30係沿一第一方向X朝向該分光鏡40發射該檢測光線。當該檢測光線經過該分光鏡40反射後，該檢測光線係沿背離一第二方向Y之方向射向該聚光鏡50。該待測物111散射出之該拉曼散射光線則係沿該第二方向Y依序通過該聚光鏡50、該分光鏡40、該濾光片60及該準直單元70後，射入該離散式光感測單元80，由該離散式光感測單元80接收經過該準直單元70準直該拉曼散射光線後產生的該準直光線。在本實施例中，該第一方向X與該第二方向Y垂直。The
進一步而言，該準直單元70包含有一第一凸透鏡71及一第二凸透鏡72。該第一凸透鏡71具有一第一焦距f1，該第二凸透鏡72具有一第二焦距f2，且該第二焦距f2係大於該第一焦距f1。此外，該第一凸透鏡71的一第一焦點的位置與該第二凸透鏡72的一第二焦距的位置相同，即該第一凸透鏡71與該第二凸透鏡具有相同的一焦點位置f。當該拉曼散射光線通過該濾光片60後，該拉曼散射光線係射向該準直單元70的該第一凸透鏡71，並通過該第一凸透鏡71聚焦後，再射向該準直單元70的該第二凸透鏡72，並通過該第二凸透鏡72準直成該準直光線。Furthermore, the
當該拉曼散射光線在進入該準直單元70時，該拉曼散射光線係先通過該第一凸透鏡71聚焦於該焦點位置f，且聚焦後的該拉曼散射光線進一步射向該第二凸透鏡72。由於第一凸透鏡71與該第二凸透鏡72具有相同的焦點位置f，因此聚焦後的該拉曼散射光線係由該第二凸透鏡72的焦點位置f射向該第二凸特鏡72，故可藉由該第二凸透鏡72將聚焦後的該拉曼散射光線準直成該準直光線。此外，由於該第二凸透鏡72的該第二焦距f2大於該第一凸透鏡71的該第一焦距f1，因此該準直光線的截面積會大於入射該準直單元70前的該拉曼散射光線的截面積。藉此，該離散式光感測單元80便可接收到截面積較大的該準直光線，進而提高感測的準確度。When the Raman scattered light enters the
請參閱圖4及圖5所示，該離散式光感測單元80包含有複數光強度感測器81及複數離散濾光片82。該些光強度感測器81係設置於一電路板83上，且通過該電路板83上的線路分別電連接該處理單元20。在本實施例中，該些光強度感測器81係呈矩陣排列。該些光強度感測器81的數量為2~25個。Please refer to FIG. 4 and FIG. 5 . The discrete
該些離散濾光片82之設置位置係分別對應該些光強度感測器81的設置位置。即該些離散濾光片82的設置位置分別與該些光強度感測器81的設置位置係一對一對應。舉例來說，該離散式光感測單元80還包含有一外殼84及一上蓋85，該電路板83上設置有該些光強度感測器81，該些光強度感測器81上方分別設置有該些離散濾光片81，並且該電路板83、該些光強度感測器81及該些離散濾光片81係共同設置於該外殼84內，並於上方蓋上該上蓋85。而該準直光線係從該上蓋85射入該外殼內84，並經過該些離散濾光片81分別濾光後，由該些光強度感測器81分別接收經過濾後的不同頻段的過濾光線。The arrangement positions of the discrete
此外，該些離散濾光片82分別具有一濾光頻段，且該些離散濾光片82的該些濾光頻段互不相同。而該準直光線係射向該些離散濾光片82，並分別通過該些離散濾光片82濾光後，分別射向該些光強度感測器81。也就是說，每一個光強度感測器81係分別接收經過其中一個離散濾光片82過濾後的該準直光線。詳細來說，每一個光強度感測器81接收到的過濾後的該準直光線分別為不同頻段。In addition, the
在本實施例中，該離散式光感測單元80的該些離散濾光片82分別係一帶通濾光片，用於讓特定頻段的光線通過。In this embodiment, the
進一步而言，該離散式光感測單元80的該些光強度感測器81包含有至少一峰值光強度感測器及至少一谷值光強度感測器。該至少一峰值光強度感測器係用於感測特徵頻段的光是否為峰值，而該至少一谷值光強度感測器係用於感測特徵頻段的光是否為谷值。該離散式光感測單元81的該些離散濾光片包含有至少一峰值濾光片及至少一谷值濾光片。該至少一峰值濾光片的設置位置對應該至少一峰值光強度感測器的設置位置，且該至少一谷值濾光片的設置位置對應該至少一谷值光強度感測器的設置位置。Furthermore, the
也就是說，該至少一峰值光強度感測器接收到的係經過該至少一峰值濾光片過濾後的該準直光線，而該至少一谷值光強度感測器接收到的係經過該至少一谷值濾光片過濾後的該準直光線。That is to say, the at least one peak light intensity sensor receives the collimated light filtered by the at least one peak filter, and the at least one valley light intensity sensor receives the collimated light that passes through the at least one peak light intensity filter. The collimated light is filtered by at least one valley filter.
此外，該離散式光感測單元80產生的該些光強度感測訊號包含有至少一峰值光強度感測訊號或至少一谷值光強度感測訊號。該至少一峰值光強度感測器根據經過該至少一峰值濾光片濾光後的該準直光線，產生該至少一峰值光強度感測訊號。該至少一谷值光強度感測器根據經過該至少一谷值濾光片濾光後的該準直光線，產生該至少一谷值光強度感測訊號。In addition, the light intensity sensing signals generated by the discrete
當該處理單元20接收到該至少一峰值光強度感測訊號及該至少一谷值光強度感測訊號時，該處理單元20係判斷該至少一峰值光強度感測訊號是否大於或等於一峰值閾值以及判斷該至少一谷值光強度感測訊號是否小於或等於一谷值閾值。When the
當該至少一峰值光強度感測訊號大於或等於該峰值閾值以及該至少一谷值光強度感測訊號小於或等於該谷值閾值時，該處理單元20產生的該檢測結果為一警示訊號，反之，該處理單元產生的該檢測結果為一安全訊號。When the at least one peak light intensity sensing signal is greater than or equal to the peak threshold and the at least one valley light intensity sensing signal is less than or equal to the valley threshold, the detection result generated by the
在另一實施例中，當該處理單元20接收到該至少一峰值光強度感測訊號及該至少一谷值光強度感測訊號時，該處理單元20係判斷該至少一峰值光強度感測訊號減去該至少一谷值光強度感測訊號後之一差值是否大於或等於一設定閾值。當該差值大於或等於該設定閾值時，該處理單元20產生的該檢測結果為一警示訊號，反之，該處理單元20產生的該檢測結果為一安全訊號。In another embodiment, when the
此外，在其他實施例中，該離散式光感測單元80亦可僅包含該至少一峰值光強度感測器或該至少一谷值光強度感測器之其中一種。該離散式光感測單元81的該些離散濾光片亦可僅包含有至少一峰值濾光片或至少一谷值濾光片之其中一種。而該處理單元20僅需判斷該至少一峰值光強度感測訊號是否大於或等於該峰值閾值或者判斷該至少一谷值光強度感測訊號是否小於或等於該谷值閾值之其中一種為真，該處理單元20即產生的該警示訊號作為該檢測結果，反之，該處理單元20則產生該安全訊號為該檢測結果。In addition, in other embodiments, the discrete
由於該些光強度感測器81所接收到的光線都是經過該些離散濾光片81過濾後，僅保留特定頻段的光線，因此，該些光強度感測器81所接收產生的光強度訊號係分別對應特定頻段的光線的光強度。而且由判斷特定頻段的光線的光強度，即可判斷該待測物111散射出的該拉曼散射光線是否符合特定內容物的拉曼散射光譜，藉此判斷該待測物111中是否包含有該特定內容物。Since the light received by the
舉例來說，當該離散分光式檢測裝置係用於檢測孔雀石綠時，該雷射發射器產生的該檢測光線係設定為波長785奈米(nm)的雷射光線，該帶阻濾光片或該低通濾光片的濾光頻段皆設定為785(nm)。該光強度感測器81的數量為9個，用於感測9個特徵點的光強度訊號。For example, when the discrete spectroscopic detection device is used to detect malachite green, the detection light generated by the laser emitter is set to a laser light with a wavelength of 785 nanometers (nm), and the band-stop filter light The filter frequency band of the film or the low-pass filter is set to 785 (nm). The number of the
也就是說，該帶阻濾光片係用於濾除波長為785(nm)的光線，並讓波長為785(nm)以外的光線通過。該低通濾光片係用於濾除波長少於785(nm)的光線，並讓波長超過785(nm)的光線通過。In other words, the band-rejection filter is used to filter out light with a wavelength of 785 (nm) and allow light with wavelengths other than 785 (nm) to pass through. This low-pass filter is used to filter out light with a wavelength less than 785 (nm) and pass light with a wavelength exceeding 785 (nm).
請參閱圖6及圖7所示，圖6係孔雀石綠的拉曼位移光譜示意圖。拉曼位移指的是入射至該待測物111的該檢測光線的波長與該待測物111散射出的該拉曼散射光線的波長的倒數差。Please refer to Figures 6 and 7. Figure 6 is a schematic diagram of the Raman shift spectrum of malachite green. The Raman shift refers to the reciprocal difference between the wavelength of the detection light incident on the
舉例來說，該檢測光線的波長為
，該待測物111散射出的該拉曼散射光線的波長為
。因此該拉曼位移(Raman shift)為：
For example, the wavelength of the detection light is , the wavelength of the Raman scattered light scattered by the
； ;
且根據圖6可知孔雀石綠在拉曼位移為315( )、530( )、900( )、1010( )以及1260( )處的光強度較強，為拉曼位移光譜的波峰值，並設定拉曼位移光譜的波峰值為特徵點。 And according to Figure 6, it can be seen that the Raman shift of malachite green is 315 ( ), 530( ), 900( ), 1010( ) and 1260( ) is stronger and is the peak value of the Raman shift spectrum, and the peak value of the Raman shift spectrum is set as the characteristic point.
如圖7所示，由於該檢測光線係的波長設定為785(nm)，因此將785(nm)作為該檢測光線的波長為 代入，分別計算在拉曼位移為315( )、530( )、900( )、1010( )以及1260( )時對應的拉曼散射的波長為805(nm)、819(nm)、845(nm)、853(nm)及871(nm)。 As shown in Figure 7, since the wavelength of the detection light system is set to 785 (nm), taking 785 (nm) as the wavelength of the detection light is Substituting, respectively, the Raman shift is calculated as 315 ( ), 530( ), 900( ), 1010( ) and 1260( ), the corresponding Raman scattering wavelengths are 805 (nm), 819 (nm), 845 (nm), 853 (nm) and 871 (nm).
故如圖4所述，該些離散濾光片82的該些峰值濾光片的濾光頻段分別設定為805(nm)、819(nm)、845(nm)、853(nm)及871(nm)。Therefore, as shown in Figure 4, the filter frequency bands of the peak filters of the
此外，根據圖6可知孔雀石綠在拉曼位移為240( )、440( )、760( )以及1140( )處的光強度較弱，為拉曼位移光譜的波谷值，並同時設定拉曼位移光譜的波谷值為特徵點。 In addition, according to Figure 6, it can be seen that the Raman shift of malachite green is 240 ( ), 440( ), 760( ) and 1140( ) is weaker and is the trough value of the Raman shift spectrum, and at the same time, the trough value of the Raman shift spectrum is set as the characteristic point.
如圖7所示，由於該檢測光線的波長係設定為785(nm)，因此將785(nm)作為該檢測光線的波長為 代入，分別計算在拉曼位移為240( )、440( )、760( )及1140( )時對應的拉曼散射的波長為800(nm)、813(nm)、835(nm)及862(nm)。 As shown in Figure 7, since the wavelength of the detection light is set to 785 (nm), taking 785 (nm) as the wavelength of the detection light is Substituting, respectively, the Raman shift is calculated as 240 ( ), 440( ), 760( ) and 1140( ), the corresponding Raman scattering wavelengths are 800 (nm), 813 (nm), 835 (nm) and 862 (nm).
故如圖4所示，該些離散濾光片82的該些谷值濾光片的濾光頻段分別設定為800(nm)、813(nm)、835(nm)及862(nm)。Therefore, as shown in FIG. 4 , the filter frequency bands of the valley filters of the
該些離散濾光片82的位置係依據該些離散濾光片82的濾光頻段設置的，且該些峰值濾光片與該些谷值濾光片係交錯設置，即與任一峰值濾光片相鄰濾光片的都是谷值濾光片。舉例來說，該些離散濾光片82的濾光頻段於第一列自左而右依序設定為805(nm)、800(nm)及871(nm)，於第二列自左而右依序設定為813(nm)、819(nm)及862(nm)，且於第三列自左而右依序設定為845(nm)、835(nm)及853(nm)。The positions of the
綜上所述，由於各種內容物分別具有其特定的拉曼散射光譜，且根據該拉曼散射光譜可知特徵點的拉曼位移，並可根據拉曼位移來設定該離散式光感測單元80的該些離散濾光片82的組合，即能夠用於判定該待測物111散射出的該拉曼散射光線是否符合特定拉曼光譜，藉此判斷其中是否含有特定內容物。因此，本發明可以用來檢測該待測物111中是否包含有特定內容物。In summary, since various contents have their own specific Raman scattering spectra, and the Raman shift of the characteristic point can be known based on the Raman scattering spectrum, the discrete
也就是說，雖然上述實施例係以檢測孔雀石綠為例說明，但當需要檢測其他化合物時，本發明可通過更換該離散式光感測單元80，更換後的該離散式光感測單元80的該些離散濾光片82具有不同的濾光頻段組合，即可用於檢測不同的特定內容物。That is to say, although the above embodiment takes the detection of malachite green as an example, when other compounds need to be detected, the present invention can replace the discrete
因此，本發明的優點在於成本低，體積小，可模組化，且可以透過更換該離散式光感測單元80的該些離散濾光片82的濾光頻段組合，滿足檢測不同特定內容物的需求。故本發明係為一種架構簡單，且可微型化的一種離散分光式運用在拉曼光譜之裝置。Therefore, the advantages of the present invention include low cost, small size, modularization, and the ability to detect different specific contents by replacing the filter frequency band combinations of the
以上所述僅是本發明的較佳實施例而已，並非對本發明做任何形式上的限制，雖然本發明已以較佳實施例揭露如上，然而並非用以限定本發明，任何熟悉本專業的技術人員，在不脫離本發明技術方案的範圍內，當可利用上述揭示的技術內容做出些許更動或修飾為等同變化的等效實施例，但凡是未脫離本發明技術方案的內容，依據本發明的技術實質對以上實施例所作的任何簡單修改、等同變化與修飾，均仍屬於本發明技術方案的範圍內。The above descriptions are only preferred embodiments of the present invention, and do not limit the present invention in any form. Although the present invention has been disclosed above in preferred embodiments, it is not intended to limit the present invention. Anyone familiar with the art Personnel, without departing from the scope of the technical solution of the present invention, can use the technical content disclosed above to make some changes or modifications to equivalent embodiments with equivalent changes. However, any content that does not depart from the technical solution of the present invention, according to the present invention Any simple modifications, equivalent changes and modifications made to the above embodiments according to the technical essence of the invention still fall within the scope of the technical solution of the present invention.
10:殼體 101:啟動按鍵 102:顯示單元 103:電源按鍵 11:待測物容置槽 111:待測物 20:處理單元 30:光源發射器 40:分光鏡 50:聚光鏡 60:濾光片 70:準直單元 71:第一凸透鏡 72:第二凸透鏡 80:離散式光感測單元 81:光強度感測器 82:離散濾光片 83:電路板 84:外殼 85:上蓋 θ:入射角 X:第一方向 Y:第二方向 f1:第一焦距 f2:第二焦距 f:焦點位置 10: Shell 101:Start button 102:Display unit 103:Power button 11: Test object holding tank 111:Object to be tested 20: Processing unit 30:Light source emitter 40: Beam splitter 50: condenser 60: Filter 70:Collimation unit 71: First convex lens 72: Second convex lens 80: Discrete light sensing unit 81:Light intensity sensor 82: Discrete filter 83:Circuit board 84: Shell 85: Upper cover θ: incident angle X: first direction Y: second direction f1: first focal length f2: second focal length f: focus position
圖1為本發明的離散分光式檢測裝置的示意圖； 圖2為本發明的離散分光式檢測裝置的方塊示意圖； 圖3為本發明的離散分光式檢測裝置的檢測方式的示意圖； 圖4為本發明的離散分光式檢測裝置的離散式光感測單元的示意圖； 圖5為本發明的離散分光式檢測裝置的離散式光感測單元的分解示意圖； 圖6為孔雀石綠的拉曼位移光譜示意圖； 圖7為本發明檢測孔雀石綠的拉曼光譜示意圖。 Figure 1 is a schematic diagram of the discrete spectroscopic detection device of the present invention; Figure 2 is a block diagram of the discrete spectroscopic detection device of the present invention; Figure 3 is a schematic diagram of the detection method of the discrete spectroscopic detection device of the present invention; Figure 4 is a schematic diagram of the discrete light sensing unit of the discrete spectroscopic detection device of the present invention; Figure 5 is an exploded schematic diagram of the discrete light sensing unit of the discrete spectroscopic detection device of the present invention; Figure 6 is a schematic diagram of the Raman shift spectrum of malachite green; Figure 7 is a schematic diagram of the Raman spectrum for detecting malachite green according to the present invention.
10:外殼 10: Shell
101:啟動按鍵 101:Start button
102:顯示單元 102:Display unit
103:電源按鍵 103:Power button
11:待測物容置槽 11: Test object holding tank
50:聚光鏡 50: condenser
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| US20030227628A1 (en) * | 2001-02-08 | 2003-12-11 | Kreimer David I. | Systems and methods for filter based spectrographic analysis |
| US20070195320A1 (en) * | 2006-02-17 | 2007-08-23 | Axsun Technologies, Inc. | Probe for tunable laser Raman spectroscopy system |
| US8699020B1 (en) * | 2011-12-16 | 2014-04-15 | Bwt Property, Inc. | Handheld Raman spectrometer |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030227628A1 (en) * | 2001-02-08 | 2003-12-11 | Kreimer David I. | Systems and methods for filter based spectrographic analysis |
| US20070195320A1 (en) * | 2006-02-17 | 2007-08-23 | Axsun Technologies, Inc. | Probe for tunable laser Raman spectroscopy system |
| US8699020B1 (en) * | 2011-12-16 | 2014-04-15 | Bwt Property, Inc. | Handheld Raman spectrometer |
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