TWI767323B

TWI767323B - Biochip detection device and method thereof

Info

Publication number: TWI767323B
Application number: TW109131419A
Authority: TW
Inventors: 馬縈嬛
Original assignee: 馬縈嬛
Priority date: 2020-09-11
Filing date: 2020-09-11
Publication date: 2022-06-11
Also published as: TW202210832A

Abstract

本發明係揭露一種生物晶片檢測裝置及其方法，包含由下至上設置的基材、反應模組以及旋轉單元。旋轉單元可於貼合後的基材與反應模組上旋轉作動。旋轉單元具有複數個滴入孔，可各別滴入不同的待測液，其滲透至反應模組以形成待測區域，再將檢測液滴入至圍繞待測區域的中心位置，檢測液會被吸附往周邊流動至待測區域以彼此形成反應，更可滴入清洗液以濾除雜質，以取得更精準的最終檢測結果。其中待測液容量僅需微量就可進行檢測，且可同時檢測多個不同待測液，又能讓檢測液以周邊擴散方式均勻的流動至待測液進行反應，可有效提升檢測精準度。 The invention discloses a biochip detection device and a method thereof, comprising a substrate, a reaction module and a rotation unit arranged from bottom to top. The rotating unit can rotate on the bonded substrate and the reaction module. The rotating unit has a plurality of dropping holes, which can respectively drop different liquids to be tested, which penetrate into the reaction module to form the area to be tested, and then drop the test droplets into the center around the area to be tested. It is adsorbed and flows to the area to be tested to form a reaction with each other, and can be dripped with cleaning solution to filter out impurities, so as to obtain more accurate final test results. The volume of the liquid to be tested can be detected by only a small amount, and multiple different liquids to be tested can be detected at the same time, and the detection liquid can be uniformly flowed to the liquid to be tested by peripheral diffusion for reaction, which can effectively improve the detection accuracy.

Description

生物晶片檢測裝置及其方法Biochip detection device and method thereof

本發明是有關於一種生物晶片檢測裝置及其方法，特別是有關於一種可同時檢測多種不同檢測資料以及提高檢測結果的精準度之技術領域。The present invention relates to a biochip testing device and method thereof, and more particularly to the technical field of simultaneously testing a variety of different testing data and improving the accuracy of testing results.

按，免疫層析技術已經在現今醫療體系上扮演相當重要的角色，可用以定性或定量量測各種生物指標的變化，以提供檢驗人員或醫療人員進行疾病診斷或藥物治療等資訊的參考。Press, immunochromatography technology has played a very important role in today's medical system. It can be used to qualitatively or quantitatively measure the changes of various biological indicators to provide information such as disease diagnosis or drug treatment for laboratory personnel or medical personnel.

目前市面上絕大多數的檢測試片係以定性設計方式讓檢測者以目測方式來得知檢驗的結果，例如中華民國專利第I561820號之『上下消化道癌篩檢驗套組及其採樣液與採樣手法』，但此前案的檢測試片僅能檢測單一項目而無法同時檢測多種不同的檢測項目。又如中華民國專利第M554567號之『多樣本快速檢測試片裝置(一)』雖然可同時檢測多種不同的檢測項目，但是檢測液要流動至多個待測液的反應區時，檢測液是以單一方向的側向流動，再依序流動至每一反應區與待測液進行反應，此方式會造成檢測液流動至每一反應區的容量會由多漸少，因為檢測液分布地的不均勻，使得待測液所接收的檢測液不同而產生檢驗誤差，甚至發生檢驗不準確的問題。因此，如何藉由創新的檢測裝置設計，能夠同時檢測多種不同檢測項目，又可提高檢測的準確度是亟待解決的問題。At present, the vast majority of test strips on the market are qualitatively designed to allow the tester to know the test results by visual inspection. method”, but the test strip of the previous case can only detect a single item and cannot detect multiple different test items at the same time. Another example is the "multi-sample rapid detection test strip device (1)" of the Republic of China Patent No. M554567, although it can detect a variety of different detection items at the same time, but when the detection liquid needs to flow to the reaction areas of multiple liquids to be tested, the detection liquid is The lateral flow in a single direction, and then flows to each reaction zone in sequence to react with the liquid to be tested. This method will cause the capacity of the test liquid to flow to each reaction zone from more to less, because the distribution of the test liquid is different. Evenly, the test liquid received by the liquid to be tested is different, resulting in inspection error, and even the problem of inaccurate inspection. Therefore, how to simultaneously detect a variety of different detection items and improve the detection accuracy through innovative detection device design is an urgent problem to be solved.

有鑑於上述習知技藝之問題，本發明之目的就是提供一種可同時檢測多種不同檢測資料，僅需微量的待測液就可與檢測液進行反應，不僅能降低採檢的困難度與大幅增加檢測樣品，又利用檢測液的周邊擴散流動方式，均勻地同時與所有待測液進行結合與反應，可有效提高檢測結果的精準度。In view of the problems of the above-mentioned conventional techniques, the object of the present invention is to provide a kind of simultaneous detection of multiple different detection data, and only a trace amount of the liquid to be detected can be reacted with the detection liquid, which can not only reduce the difficulty of collecting and detect and greatly increase. The detection sample uses the peripheral diffusion flow mode of the detection liquid to uniformly combine and react with all the liquids to be tested at the same time, which can effectively improve the accuracy of the detection results.

根據本發明之目的，提出一種生物晶片檢測裝置，包含一基材、一反應模組以及一旋轉單元。反應模組係貼合基材上，反應模組包括由下至上層疊的一反應層、一吸液層與一固定層。反應層鄰近基材，吸液層具有一吸液開孔與位於吸液開孔周邊的一吸液區域。固定層具有一反應開孔，反應開孔與吸液開孔形成相連通。旋轉單元設置於反應模組上，旋轉單元具有一本體、位於本體上之一樞軸件及複數個滴入孔。樞軸件連接反應模組與基材，旋轉單元利用樞軸件於反應模組上旋轉作動，該些滴入孔的位置對應反應開孔，且彼此相連通。According to the purpose of the present invention, a biochip detection device is provided, which includes a substrate, a reaction module and a rotation unit. The reaction module is attached to the substrate, and the reaction module includes a reaction layer, a liquid absorption layer and a fixing layer stacked from bottom to top. The reaction layer is adjacent to the substrate, and the liquid-absorbing layer has a liquid-absorbing opening and a liquid-absorbing area located around the liquid-absorbing opening. The fixed layer has a reaction opening, and the reaction opening is in communication with the liquid absorption opening. The rotating unit is arranged on the reaction module, and the rotating unit has a main body, a pivot member located on the main body and a plurality of drip holes. The pivot element connects the reaction module and the base material, and the rotating unit uses the pivot element to rotate on the reaction module. The positions of the drop holes correspond to the reaction openings and are communicated with each other.

依據上述技術特徵，其中該些滴入孔之側邊分別具有不同的一滴注標記。According to the above technical features, the sides of the dripping holes respectively have a different dripping mark.

依據上述技術特徵，其中該本體具有一滴入區域，該些滴入孔位於滴入區域內，並以滴入區域的中心位置向外呈間隔環繞設置。According to the above technical features, the body has a drop-in area, and the drop-in holes are located in the drop-in area, and are arranged around the center of the drop-in area at intervals.

依據上述技術特徵，其中該本體具有一滴入區域，該些滴入孔位於滴入區域內，並以滴入區域的中心位置向外呈彼此交錯間隔設置。According to the above technical features, the body has a drop-in area, and the drop-in holes are located in the drop-in area and are staggered and spaced outward from the center of the drop-in area.

依據上述技術特徵，其中該本體具有一滴入區域，該些滴入孔位於滴入區域內，該些滴入孔係環繞設置為至少兩個環繞區域，區分為一第一環繞區域與一第二環繞區域，位於第一環繞區域與第二環繞區域的該些滴入孔彼此交錯設置。According to the above technical features, wherein the body has a dripping area, the dripping holes are located in the dripping area, and the dripping holes are arranged around at least two surrounding areas, which are divided into a first surrounding area and a first surrounding area. Two surrounding areas, the dripping holes located in the first surrounding area and the second surrounding area are staggered with each other.

依據上述技術特徵，其中該反應開孔的孔徑大於吸液開孔的孔徑，且吸液區域部分裸露於反應開孔。According to the above technical features, the pore size of the reaction opening is larger than that of the liquid absorption opening, and the liquid absorption area is partially exposed to the reaction opening.

依據上述技術特徵，其中該反應層的尺寸小於吸液層的尺寸，反應層抵觸吸液開孔與局部吸液區域。According to the above technical features, the size of the reaction layer is smaller than the size of the liquid absorption layer, and the reaction layer interferes with the liquid absorption opening and the local liquid absorption area.

依據上述技術特徵，其中該樞軸件具有一旋轉自由端與一固定端，固定端用以固定基材的底面，旋轉自由端位於旋轉單元之本體上。According to the above technical features, the pivot member has a free rotating end and a fixed end, the fixed end is used for fixing the bottom surface of the base material, and the free rotating end is located on the body of the rotating unit.

依據上述技術特徵，其中該基材具有一第一樞接孔，反應模組之固定層具有一第二樞接孔，旋轉單元之本體具有一第三樞接孔，樞軸件係貫穿樞接於第一樞接孔、第二樞接孔與第三樞接孔。According to the above technical features, wherein the base material has a first pivot hole, the fixed layer of the reaction module has a second pivot hole, the body of the rotating unit has a third pivot hole, and the pivot member is pivoted through. in the first pivot hole, the second pivot hole and the third pivot hole.

依據上述技術特徵，其中該檢測液包含反應抗體與膠體金。According to the above technical features, the detection solution contains reactive antibodies and colloidal gold.

根據本發明之目的，提出一種生物晶片檢測裝置，包含一基材、一反應模組以及一旋轉單元。反應模組係貼合基材上，反應模組包括由下至上層疊的一反應層、一吸液層與一固定層。反應層鄰近基材，吸液層具有一吸液開孔與位於吸液開孔周邊的一吸液區域。固定層具有一反應開孔，反應開孔與吸液開孔形成相連通。旋轉單元設置於反應模組上，旋轉單元具有一本體、位於本體上之一樞軸件及複數個滴入孔。樞軸件連接反應模組與基材，旋轉單元利用樞軸件於反應模組上旋轉作動，該些滴入孔的位置對應反應開孔，且彼此相連通。其中，每一滴入孔係供一待測液滴入並滲透經反應開孔、吸液開孔至反應層形成一待測區域。於本體藉由樞軸件旋轉遠離反應模組時，反應開孔與吸液開孔係供一檢測液滴入至圍繞待測區域的中心位置，藉由吸液區域將檢測液吸附流動至待測區域以彼此形成反應，並取得最終檢測結果。According to the purpose of the present invention, a biochip detection device is provided, which includes a substrate, a reaction module and a rotation unit. The reaction module is attached to the substrate, and the reaction module includes a reaction layer, a liquid absorption layer and a fixing layer stacked from bottom to top. The reaction layer is adjacent to the substrate, and the liquid-absorbing layer has a liquid-absorbing opening and a liquid-absorbing area located around the liquid-absorbing opening. The fixed layer has a reaction opening, and the reaction opening is in communication with the liquid absorption opening. The rotating unit is arranged on the reaction module, and the rotating unit has a main body, a pivot member located on the main body and a plurality of drip holes. The pivot element connects the reaction module and the base material, and the rotating unit uses the pivot element to rotate on the reaction module. The positions of the drop holes correspond to the reaction openings and are communicated with each other. Wherein, each drop-in hole is for a drop to be tested and penetrated through the reaction opening, the liquid suction opening to the reaction layer to form an area to be measured. When the body is rotated away from the reaction module by the pivot member, the reaction opening and the suction opening are for a detection droplet to drop into the center around the area to be measured, and the detection solution is adsorbed and flowed to the area to be measured by the suction area. The test areas can form a reaction with each other and obtain the final test result.

依據上述技術特徵，其中該待測液之容量介於0.1微克至0.2微克。According to the above technical features, the volume of the liquid to be tested is between 0.1 micrograms and 0.2 micrograms.

依據上述技術特徵，其中該旋轉單元係為一透明基材。According to the above technical features, the rotating unit is a transparent substrate.

根據本發明之另一目的，提出一種生物晶片檢測方法，包含將一基材、一反應模組以及一旋轉單元由下至上設置，且基材與反應模組為相貼合固定。於旋轉單元之複數個滴入孔中的至少一個滴入孔滴入一待測液，待測液滲透反應模組以形成一待測區域。旋轉旋轉單元遠離反應模組與基材。於圍繞待測區域的中心位置滴入一檢測液，使檢測液與待測液彼此反應以產生一初次檢測結果。於待測區域滴入一清洗液，濾除初次檢測結果中的雜質，據以取得一最終檢測結果，例如呈色檢測結果。According to another object of the present invention, a biochip detection method is provided, which includes arranging a substrate, a reaction module, and a rotating unit from bottom to top, and the substrate and the reaction module are attached and fixed. A liquid to be tested is dropped into at least one of the plurality of dropping holes of the rotating unit, and the liquid to be tested penetrates the reaction module to form an area to be tested. The rotating rotating unit is away from the reaction module and the substrate. A detection liquid is dropped into the center around the area to be tested, so that the detection liquid and the liquid to be tested react with each other to generate an initial detection result. A cleaning solution is dropped into the area to be tested to filter out impurities in the initial test result, so as to obtain a final test result, such as a color test result.

依據上述技術特徵，其中於滴入待測液的步驟之後，更包括一乾燥步驟，乾燥時間介於5-15分鐘。According to the above technical features, after the step of dropping the liquid to be tested, a drying step is further included, and the drying time is between 5-15 minutes.

依據上述技術特徵，其中於滴入檢測液的步驟之後，更包括一乾燥步驟，乾燥時間介於5-15分鐘。According to the above technical features, after the step of dropping the detection liquid, a drying step is further included, and the drying time is between 5-15 minutes.

依據上述技術特徵，其中該清洗液係為純水。According to the above technical features, the cleaning liquid is pure water.

依據上述技術特徵，其中該些滴入孔之側邊分別具有不同的一滴注標記，滴注標記係對應待測液的檢測資料。According to the above technical features, the sides of the dripping holes respectively have a different dripping mark, and the dripping mark corresponds to the detection data of the liquid to be tested.

依據上述技術特徵，其中該檢測資料包含受測者資料、檢體及檢測項目。According to the above technical features, the testing data includes the data of the subject, the specimen and the testing items.

依據上述技術特徵，其中該旋轉單元係由透明材料所製成的透明基材。According to the above technical features, the rotating unit is a transparent substrate made of a transparent material.

為利貴審查員瞭解本發明之技術特徵、內容與優點及其所能達成之功效，茲將本發明配合附圖，並以實施例之表達形式詳細說明如下，而其中所使用之圖式，其主旨僅為示意及輔助說明書之用，未必為本發明實施後之真實比例與精準配置，故不應就所附之圖式的比例與配置關係解讀、侷限本發明於實際實施上的權利範圍，合先敘明。In order to facilitate the examiners to understand the technical features, content and advantages of the present invention and the effects that can be achieved, the present invention is hereby described in detail with the accompanying drawings and in the form of embodiments as follows. The subject matter is only for illustration and auxiliary description, and is not necessarily the real scale and precise configuration after the implementation of the present invention. Therefore, the ratio and configuration relationship of the attached drawings should not be interpreted or limited to the scope of rights of the present invention in actual implementation. Together first to describe.

請同時參閱第1圖、第2圖、第3圖及第4圖，第1圖為本發明之生物晶片檢測裝置之結構示意圖；第2圖為第1圖之結構分解圖；第3圖為第1圖沿A-A’剖面線之結構剖視圖；第4圖為本發明之旋轉單元之第一實施例結構示意圖。本發明的生物晶片檢測裝置100是應用於免疫層析技術，首先，先說明本發明的結構創新設計，生物晶片檢測裝置100包括一基材10、一反應模組20以及一旋轉單元30。反應模組20係貼合於基材10上，旋轉單元30設置於反應模組20上。反應模組20包括由下至上層疊的一反應層21、一吸液層22與一固定層23，反應層21鄰近基材10，固定層23鄰近旋轉單元30。吸液層22具有一吸液開孔221與位於吸液開孔221周邊的一吸液區域222，固定層23具有一反應開孔231，反應開孔231與吸液開孔221形成相連通。Please refer to Figure 1, Figure 2, Figure 3 and Figure 4 at the same time, Figure 1 is a schematic diagram of the structure of the biochip detection device of the present invention; Figure 2 is an exploded view of the structure of Figure 1; Figure 3 is FIG. 1 is a cross-sectional view of the structure along the section line AA'; FIG. 4 is a schematic view of the structure of the rotating unit according to the first embodiment of the present invention. The biochip detection device 100 of the present invention is applied to immunochromatography technology. First, the innovative structural design of the present invention is described. The biochip detection device 100 includes a substrate 10 , a reaction module 20 and a rotation unit 30 . The reaction module 20 is attached to the substrate 10 , and the rotating unit 30 is disposed on the reaction module 20 . The reaction module 20 includes a reaction layer 21 , a liquid absorption layer 22 and a fixed layer 23 stacked from bottom to top. The reaction layer 21 is adjacent to the substrate 10 , and the fixed layer 23 is adjacent to the rotating unit 30 . The liquid-absorbing layer 22 has a liquid-absorbing opening 221 and a liquid-absorbing area 222 located around the liquid-absorbing opening 221 .

其中，反應開孔231的孔徑大於吸液開孔221的孔徑，且吸液區域222部分裸露於反應開孔231，有助於檢測者直視觀看檢測的反應過程與狀態。反應層21的尺寸小於吸液層22的尺寸，反應層21抵觸吸液開孔221與局部吸液區域222，反應層21只要能涵蓋吸液開孔221的範圍，確保欲滴入的液體(例如待測液、檢測液及清洗液等)能夠落入反應層21即可，反應層21可以為硝化纖維膜(nitro-cellulose membrane)、PVDF膜〔polyvinylidone(PVDF)membrane〕、尼龍膜(nylon membrane)等，係用以吸附待測液40中的所欲檢測的蛋白質。The diameter of the reaction opening 231 is larger than that of the liquid suction opening 221 , and the liquid suction area 222 is partially exposed to the reaction opening 231 , which is helpful for the inspector to directly observe the reaction process and state of the detection. The size of the reaction layer 21 is smaller than the size of the liquid absorption layer 22, and the reaction layer 21 interferes with the liquid absorption opening 221 and the partial liquid absorption area 222. As long as the reaction layer 21 can cover the range of the liquid absorption opening 221, the liquid ( For example, the liquid to be tested, detection liquid, cleaning liquid, etc. can fall into the reaction layer 21. The reaction layer 21 can be a nitro-cellulose membrane, a PVDF membrane (polyvinylidone (PVDF) membrane), a nylon membrane (nylon) membrane), etc., are used to adsorb the protein to be detected in the liquid 40 to be detected.

旋轉單元30具有一本體31、位於本體31上之一樞軸件32及複數個滴入孔33。旋轉單元30係為一透明基材。樞軸件32連接反應模組20與基材10，旋轉單元30利用樞軸件32於反應模組20上旋轉作動，該些滴入孔33的位置對應反應開孔231，且彼此相連通。其中樞軸件32具有一旋轉自由端321與一固定端322，固定端322用以固定基材10的底面，旋轉自由端321位於旋轉單元30之本體31上，使得本體31能夠在結合後基材10與反應模組20上水平旋轉，旋轉角度不受限，可依據檢測者需求操作。具體而言，基材10具有一第一樞接孔11，反應模組20之固定層23具有一第二樞接孔232，旋轉單元30之本體31具有一第三樞接孔35。利用樞軸件32係貫穿樞接於第一樞接孔11、第二樞接孔232與第三樞接孔35，使得基材10、反應模組20與旋轉單元30之一側能夠彼此相連接。The rotating unit 30 has a main body 31 , a pivot member 32 located on the main body 31 , and a plurality of drip holes 33 . The rotating unit 30 is a transparent substrate. The pivot member 32 connects the reaction module 20 and the substrate 10 . The rotation unit 30 uses the pivot member 32 to rotate on the reaction module 20 . The positions of the drop holes 33 correspond to the reaction openings 231 and communicate with each other. The pivot member 32 has a free rotating end 321 and a fixed end 322. The fixed end 322 is used to fix the bottom surface of the substrate 10, and the free rotating end 321 is located on the body 31 of the rotating unit 30, so that the body 31 can be assembled The material 10 and the reaction module 20 are rotated horizontally, and the rotation angle is not limited, and can be operated according to the needs of the inspector. Specifically, the substrate 10 has a first pivot hole 11 , the fixed layer 23 of the reaction module 20 has a second pivot hole 232 , and the body 31 of the rotating unit 30 has a third pivot hole 35 . The pivot member 32 is pivotally connected through the first pivot hole 11 , the second pivot hole 232 and the third pivot hole 35 , so that one side of the substrate 10 , the reaction module 20 and the rotating unit 30 can face each other. connect.

值得注意的是，本體31具有一滴入區域F，該些滴入孔33位於滴入區域F內，並以滴入區域F的中心位置向外呈間隔環繞設置。或者是該些滴入孔33以滴入區域F的中心位置向外呈彼此交錯間隔設置。如第４圖所示，在本實施例中，滴入區域F具有四個滴入孔33，且彼此間隔環繞設置，最佳設計是四個滴入孔33為等距。為能達到同時檢測不同的檢測資料，故該些滴入孔33之側邊分別具有不同的一滴注標記34，例如數字編號、圖案或是英文字母等滴注標記34，本發明在此不加侷限滴注標記34的型態。滴注標記34係對應待測液40的一檢測資料，檢測資料包含受測者資料、檢體及檢測項目等相關資料，如此一來，檢測者可藉由滴注標記34清楚辨識每一個所對應的檢測資料，能有效防止檢測者的作業疏失。It is worth noting that the body 31 has a dripping area F, and the dripping holes 33 are located in the dripping area F, and are arranged around the center of the dripping area F at intervals. Alternatively, the dripping holes 33 are arranged at a staggered interval from the center of the dripping region F outward. As shown in FIG. 4 , in this embodiment, the dripping area F has four dripping holes 33 , which are arranged around each other at intervals, and the optimal design is that the four dripping holes 33 are equidistant. In order to detect different detection data at the same time, the sides of the drip holes 33 respectively have a different drip mark 34, such as a number number, a pattern or an English letter, etc. Add the type of limited drip mark 34. The drip mark 34 is a test data corresponding to the liquid to be tested 40 , and the test data includes relevant data such as the subject's data, the specimen, and the test items. In this way, the tester can clearly identify each object by the drip mark 34 . Corresponding inspection data can effectively prevent inspectors from negligent work.

請同時參閱第5圖，其為本發明於滴入待測液之結構狀態圖。每一滴入孔33係供一待測液40滴入，待測液40可以為體液或血液，在此以四個滴入孔33為例說明，每一個滴入孔33所滴入的待測液40皆不同，屬於不同的檢測資料，其中受測者資料與檢體不同，但是檢測項目可為相同，也可為不相同，可根據檢測需求作適當的調整。Please also refer to FIG. 5 , which is a structural state diagram of the present invention when the liquid to be tested is dropped. Each dropping hole 33 is for dropping a liquid to be tested 40. The liquid to be tested 40 may be body fluid or blood. Here, four dropping holes 33 are used as an example to illustrate. The test liquids 40 are all different and belong to different test data, wherein the test subject data and the specimen are different, but the test items can be the same or different, and can be adjusted appropriately according to the test requirements.

再如第6圖所示，為本發明於旋轉單元旋轉後之結構狀態圖。由於該些滴入孔33的位置對應反應開孔231，且彼此相連通，因此待測液40自滴入孔33滴入後，待測液40會滲透經反應開孔231、吸液開孔221至反應層21以形成一待測區域，此時可以操作旋轉單元30，本體31藉由樞軸件32的旋轉自由端321進行水平旋轉一角度，使旋轉單元30遠離反應模組20。此時，滴入四個待測液40(如滴注標記34所呈現數字編號1、2、3、4)後，檢測者可以直接觀看於反應層21上形成的四個待測區域如區域A1、A2、A3、A4。As shown in FIG. 6 , it is a structural state diagram of the present invention after the rotating unit is rotated. Since the positions of the dripping holes 33 correspond to the reaction openings 231 and are connected to each other, after the liquid to be tested 40 is dripped from the dripping holes 33 , the liquid to be tested 40 will penetrate through the reaction openings 231 and the liquid suction openings 221 to the reaction layer 21 to form an area to be tested. At this time, the rotating unit 30 can be operated. The main body 31 is rotated horizontally by an angle through the free rotating end 321 of the pivot member 32 to keep the rotating unit 30 away from the reaction module 20 . At this time, after dropping four liquids to be tested 40 (such as the numbers 1, 2, 3, and 4 represented by the drip marks 34 ), the inspector can directly view the four regions to be tested formed on the reaction layer 21 , such as regions A1, A2, A3, A4.

接著請參閱第7圖，為本發明於滴入檢側液之結構狀態圖。反應開孔231與吸液開孔221係供一檢測液50滴入至圍繞區域A1、A2、A3、A4的中心位置，藉由吸液區域222將檢測液50吸附朝向周圍擴散流動至區域A1、A2、A3、A4以彼此形成反應。其中，吸液區域222位於吸液開孔221的周圍，因此檢測液50受到吸液區域222的毛細作用，檢測液50以自由角度均勻且同時朝向區域A1、A2、A3、A4流動直至吸液區域222，使得所有的區域A1、A2、A3、A4能均勻接收到檢測液50，讓檢測液50與檢測液50的待測液40產生結合與反應。值得注意的是，待測液40之容量介於0.1微克至0.2微克，本發明僅需要微量的待測液40即可進行後續的檢測反應，相較於習知檢測試片所需要的待測液容量高達1毫克以上才能檢測，本發明確實能降低採檢的困難度與大幅增加檢測樣品之功效。Next, please refer to FIG. 7 , which is a structural state diagram of the present invention when the liquid on the detection side is dripped. The reaction opening 231 and the suction opening 221 are for a detection solution 50 to drop into the center of the surrounding areas A1, A2, A3, and A4, and the detection solution 50 is adsorbed and diffused to the area A1 by the suction area 222. , A2, A3, A4 to form a reaction with each other. Among them, the liquid suction area 222 is located around the liquid suction opening 221, so the detection liquid 50 is subjected to the capillary action of the liquid suction area 222, and the detection liquid 50 flows uniformly and simultaneously toward the areas A1, A2, A3, and A4 at a free angle until the liquid is absorbed. The area 222 enables all areas A1 , A2 , A3 , and A4 to receive the detection liquid 50 uniformly, so that the detection liquid 50 and the to-be-tested liquid 40 of the detection liquid 50 are combined and reacted. It is worth noting that the capacity of the liquid to be tested 40 is between 0.1 micrograms and 0.2 micrograms, and the present invention only needs a small amount of the liquid to be tested 40 to perform subsequent detection reactions, which is compared with the conventional detection test pieces. Only when the liquid volume is as high as 1 mg or more can be detected, the present invention can indeed reduce the difficulty of collecting and testing and greatly increase the efficiency of testing samples.

其中，檢測液50包含反應抗體與膠體金兩者混合而成，其中，膠體金與反應抗體事先結合並保留反應抗體能與所欲檢測的蛋白質結合的區域。膠體金在生物醫學個研究領域中，特別是醫學檢驗中得到日益廣泛的應用。膠體金實質上是蛋白質等高分子被吸附到膠體金顆粒表面的包被過程，也就是膠體金顆粒表面的負電荷與蛋白質，例如葡萄球菌A蛋白、免疫球蛋白、毒素、醣蛋白、酶、抗生素、激素等非共價的正電荷基團因靜電吸附而形成牢固結合。由於毛細作用，檢測液50沿著吸附體吸液區域222方向移動，當移動至有區域A1、A2、A3、A4時，檢測液50中所欲檢測的蛋白質會與待測液40中的反應抗體產生抗體與抗原的專一性結合反應。The detection solution 50 includes a mixture of reactive antibody and colloidal gold, wherein the colloidal gold and the reactive antibody are combined in advance and the region where the reactive antibody can bind to the protein to be detected is reserved. Colloidal gold has been widely used in biomedical research fields, especially in medical testing. Colloidal gold is essentially a coating process in which proteins and other macromolecules are adsorbed to the surface of colloidal gold particles, that is, the negative charge on the surface of colloidal gold particles and proteins, such as Staphylococcus protein A, immunoglobulins, toxins, glycoproteins, enzymes, Non-covalent positively charged groups such as antibiotics and hormones form strong bonds due to electrostatic adsorption. Due to capillary action, the detection liquid 50 moves along the direction of the adsorbent liquid absorption area 222 , and when it moves to the areas A1 , A2 , A3 , and A4 , the protein to be detected in the detection liquid 50 will react with the liquid to be tested 40 . Antibodies produce specific binding reactions between antibodies and antigens.

如第8圖與如第9圖所示，為本發明於滴入清洗液之結構狀態圖以及本發明顯示反應檢測結果之結構狀態圖。承上段所述，檢測液50與區域A1、A2、A3、A4的待測液40進行抗體與抗原的專一性結合反應，而呈現結合反應現象即稱為陽性反應，也就是會有較多含有膠體金的抗體與檢測液50中所欲檢測的蛋白質進行結合反應；反之，無結合反應現象則稱為陰性反應，也就是不會出現有抗體與抗原的專一性結合反應。於實際實施時，可藉由吸液區域222將檢測液50吸附朝向周圍擴散流動至區域A1、A2、A3、A4以彼此形成反應，使檢測液50的液體能均勻地散布，而不至於集中於某一處，進一步地使得檢測精準度能大幅提升。As shown in FIG. 8 and FIG. 9, it is a structural state diagram of the present invention in which the cleaning solution is dripped and a structural state diagram of the present invention showing the reaction detection result. As described in the previous paragraph, the detection solution 50 and the test solution 40 in the regions A1, A2, A3, and A4 carry out the specific binding reaction of the antibody and the antigen, and the phenomenon of the binding reaction is called a positive reaction, that is, there will be more The antibody of the colloidal gold undergoes a binding reaction with the protein to be detected in the detection solution 50; otherwise, the phenomenon of no binding reaction is called a negative reaction, that is, there will be no specific binding reaction between the antibody and the antigen. In actual implementation, the detection liquid 50 can be adsorbed by the liquid suction region 222 and diffused and flowed to the regions A1, A2, A3, and A4 to form a reaction with each other, so that the liquid of the detection liquid 50 can be uniformly dispersed without being concentrated. At a certain point, the detection accuracy can be further greatly improved.

如第9圖與第10圖所示，為本發明於滴入清洗液之結構狀態圖以及本發明另一顯示反應檢測結果之結構狀態圖。於圍繞區域A1、A2、A3、A4的中心位置滴入清洗液60，藉由吸液區域222將清洗液60吸附朝向周圍擴散流動至區域A1、A2、A3、A4，可將區域A1、A2、A3、A4中未與待測液40進行抗體與抗原的專一性結合反應的檢測液50濾除，有助於提高檢測之精準度。As shown in FIG. 9 and FIG. 10, it is a structural state diagram of the present invention in which the cleaning solution is dripped and another structural state diagram of the present invention showing the reaction detection result. The cleaning solution 60 is dropped into the center around the areas A1, A2, A3, and A4, and the cleaning solution 60 is adsorbed by the suction area 222 and diffused toward the surrounding area to flow to the areas A1, A2, A3, and A4. The detection solution 50 in A3 and A4 that has not undergone the specific binding reaction of antibody and antigen with the solution to be tested 40 is filtered out, which helps to improve the detection accuracy.

如第9圖所示，區域A1、A2以及A3因含有大量膠體金聚集而呈現相對於其他區域更為顯著的顏色表現，而如第10圖所示，藉由滴入清洗液60後，僅留下區域A2是出現顏色表現。由此可見，未使用清洗液60之前，區域A1、A3屬於僞陽性現象。因此，本發明使用清洗液60確實可讓最終檢測結果更加準確。As shown in Fig. 9, the regions A1, A2 and A3 show more significant color performance than other regions due to the aggregation of a large amount of colloidal gold. As shown in Fig. 10, after dripping the cleaning solution 60, only Leaving the area A2 is where the color expression appears. It can be seen that, before the cleaning solution 60 is not used, the areas A1 and A3 belong to false positive phenomena. Therefore, the use of the cleaning liquid 60 in the present invention can indeed make the final detection result more accurate.

除了上述第一實施例使用四個滴入孔33結構設計之外，再如第11圖所示，為本發明另一顯示反應檢測結果之結構狀態圖。本體31具有一滴入區域F，該些滴入孔33位於滴入區域F內，並以滴入區域F的中心位置向外呈間隔環繞設置。由於待測液40之容量介於0.1微克至0.2微克，僅需非常微量就可以進行檢測，因此該些滴入孔33的孔徑設計可以更小，且滴入孔33數量可以在有限的檢測空間達到最大量化。又如第12圖所示，為本發明另一顯示反應檢測結果之結構狀態圖。該些滴入孔33位於滴入區域F內，該些滴入孔33係環繞設置為至少兩個環繞區域，區分為一第一環繞區域S1與一第二環繞區域S2。第一環繞區域S1鄰近滴入區域F的心中位置，第二環繞區域S2遠離滴入區域F的心中位置，位於第一環繞區域S1與第二環繞區域S2的該些滴入孔33彼此交錯設置。滴入孔33利用交錯設置設計，可以讓檢測液50滴入至圍繞多個待測區域的中心位置時，藉由吸液區域222將檢測液50吸附朝向周圍擴散流動至所有待測區域以彼此形成反應，可以讓檢測液50流動至所有待測區域達到均勻性，不會因為大幅增加滴入孔33數量而影響到檢測結果。In addition to the above-mentioned first embodiment using the structure design of four dropping holes 33 , as shown in FIG. 11 , it is another structural state diagram showing the reaction detection result of the present invention. The body 31 has a dripping area F, and the dripping holes 33 are located in the dripping area F, and are arranged around the center of the dripping area F at intervals. Since the capacity of the liquid to be tested 40 is between 0.1 micrograms and 0.2 micrograms, only a very small amount can be detected, so the pore size of the dripping holes 33 can be designed to be smaller, and the number of dripping holes 33 can be limited in the detection space. to the maximum quantification. Also as shown in FIG. 12 , it is another structural state diagram showing the reaction detection result of the present invention. The dripping holes 33 are located in the dripping area F, and the dripping holes 33 are arranged around at least two surrounding areas, which are divided into a first surrounding area S1 and a second surrounding area S2. The first surrounding area S1 is adjacent to the center of the dripping area F, the second surrounding area S2 is far from the center of the dripping area F, and the dripping holes 33 located in the first surrounding area S1 and the second surrounding area S2 are staggered with each other. . The drop-in hole 33 is designed in a staggered arrangement, so that when the detection liquid 50 is dropped into the center around the multiple test areas, the test liquid 50 is absorbed by the liquid suction area 222 and diffused toward the surrounding area to flow to all the test areas to avoid each other. By forming the reaction, the detection liquid 50 can flow to all the regions to be tested to achieve uniformity, and the detection result will not be affected due to the large increase in the number of the dripping holes 33 .

請同時參閱第2圖、第5圖、第7圖、第9圖與第13圖，第13圖為本發明之步驟流程圖。如步驟B1，將一基材10、一反應模組20以及一旋轉單元30由下至上設置，且基材10與反應模組20為相貼合固定。如步驟B2，於旋轉單元30之複數個滴入孔33中的至少一個滴入孔33滴入一待測液40，待測液40滲透反應模組20以形成一待測區域。於滴入待測液40的步驟之後，更包括一乾燥步驟(圖中未示)，乾燥時間介於5-15分鐘，此乾燥時間是作為形成待測區域的反應時間。再如步驟B3，旋轉旋轉單元30遠離反應模組20與基材10。如步驟B4，於圍繞待測區域的中心位置滴入一檢測液50，使檢測液50與待測液40彼此反應以產生一初次檢測結果。其中於滴入檢測液50的步驟之後，更包括一乾燥步驟(圖中未示)，乾燥時間介於5-15分鐘，此乾燥時間是作為檢測液50與待測液40產生結合與反應的反應時間。檢測液50包含反應抗體與膠體金，其中，膠體金與反應抗體事先結合並保留反應抗體能與所欲檢測的蛋白質結合的區域，由於上述實施例已詳述過檢測液50的組成成分與作用，故在此不在贅述。如步驟B5，於待測區域滴入一清洗液60，清洗液60係為純水，清洗液60用以濾除初次檢測結果中的雜質，據以取得一最終檢測結果。Please refer to Fig. 2, Fig. 5, Fig. 7, Fig. 9 and Fig. 13 at the same time, and Fig. 13 is a flow chart of the steps of the present invention. In step B1 , a substrate 10 , a reaction module 20 and a rotating unit 30 are arranged from bottom to top, and the substrate 10 and the reaction module 20 are attached and fixed. In step B2, a liquid to be tested 40 is dropped into at least one of the plurality of instillation holes 33 of the rotating unit 30, and the liquid to be tested 40 penetrates the reaction module 20 to form an area to be tested. After the step of dropping the liquid to be tested 40, a drying step (not shown in the figure) is further included, and the drying time is 5-15 minutes, and the drying time is used as the reaction time for forming the area to be tested. In step B3 again, the rotating unit 30 is rotated away from the reaction module 20 and the substrate 10 . In step B4, a detection liquid 50 is dropped into the center around the area to be tested, so that the detection liquid 50 and the liquid to be tested 40 react with each other to generate an initial detection result. Wherein, after the step of dropping the detection liquid 50, a drying step (not shown in the figure) is further included, and the drying time is between 5-15 minutes. Reaction time. The detection solution 50 contains the reactive antibody and colloidal gold, wherein the colloidal gold and the reactive antibody are combined in advance and retain the region where the reactive antibody can bind to the protein to be detected. Since the above-mentioned embodiment has already described the composition and function of the detection solution 50 in detail. , so it is not repeated here. In step B5, a cleaning solution 60 is dropped into the area to be tested. The cleaning solution 60 is pure water. The cleaning solution 60 is used to filter out impurities in the initial detection result, thereby obtaining a final detection result.

綜觀上述，可見本發明在突破先前之技術下，對結構上的創新改良與檢測方法，可同時檢測多種不同檢測資料，僅需微量的待測液就可與檢測液進行反應，不僅能降低採檢的困難度與大幅增加檢測樣品，又利用檢測液的周邊擴散流動方式，均勻地同時與所有待測液進行結合與反應，此外更利用清洗液提升檢測結果的精確性，整體可有效提高檢測結果的精準度，確實已達到所欲增進之功效，且也非熟悉該項技藝者所易於思及，再者，本發明申請前未曾公開，且其所具之進步性、實用性，顯已符合專利之申請要件，爰依法提出專利申請，懇請貴局核准本件發明專利申請案，以勵發明，至感德便。Looking at the above, it can be seen that the present invention breaks through the previous technology, and the innovative improvement and detection method of the structure can detect a variety of different detection data at the same time. The difficulty of detection and greatly increase the detection sample, and the peripheral diffusion flow of the detection liquid is used to uniformly combine and react with all the liquids to be tested at the same time. In addition, the cleaning liquid is used to improve the accuracy of the detection results, which can effectively improve the overall detection. The accuracy of the results has indeed achieved the desired improvement effect, and it is not easy for those who are familiar with the technology to think. Furthermore, the present invention has not been disclosed before the application, and its progressiveness and practicability are obvious. If you meet the application requirements for a patent, you can file a patent application in accordance with the law. I urge your bureau to approve this patent application for invention, in order to encourage invention.

以上所述之實施例僅係為說明本發明之技術思想及特點，其目的在使熟習此項技藝之人士能夠瞭解本發明之內容並據以實施，當不能以之限定本發明之專利範圍，即大凡依本發明所揭示之精神所作之均等變化或修飾，仍應涵蓋在本發明之專利範圍內。The above-mentioned embodiments are only to illustrate the technical ideas and characteristics of the present invention, and the purpose is to enable those who are familiar with the art to understand the content of the present invention and implement it accordingly. It should not be used to limit the patent scope of the present invention. That is, all equivalent changes or modifications made according to the spirit disclosed in the present invention should still be covered within the patent scope of the present invention.

100:生物晶片檢測裝置 10:基材 11:第一樞接孔 20:反應模組 21:反應層 22:吸液層 221:吸液開孔 222:吸液區域 23:固定層 231:反應開孔 232:第二樞接孔 30:旋轉單元 31:本體 32:樞軸件 321:旋轉自由端 322:固定端 33:滴入孔 34:滴注標記 35:第三樞接孔 40:待測液 50:檢測液 60:清洗液 F:滴入區域 A1、A2、A3、A4:區域 S1:第一環繞區域 S2:第二環繞區域 B1、B 2、B 3、B 4、B5:步驟100: Biochip detection device 10: Substrate 11: The first pivot hole 20: React Mods 21: Reactive Layer 22: Absorbent layer 221: suction opening 222: suction area 23: Fixed layer 231: reaction opening 232: Second pivot hole 30: Rotary unit 31: Ontology 32: Pivot piece 321: Rotating free end 322: Fixed end 33: Drip hole 34: Drip markers 35: The third pivot hole 40: liquid to be tested 50: detection liquid 60: Cleaning solution F: dripping area A1, A2, A3, A4: Area S1: First Surrounding Area S2: Second Surround Area B1, B2, B3, B4, B5: Steps

第1圖為本發明之生物晶片檢測裝置之結構示意圖。第2圖為第1圖之結構分解圖。第3圖為第1圖沿A-A’剖面線之結構剖視圖。第4圖為本發明之旋轉單元之第一實施例結構示意圖。第5圖為本發明於滴入待測液之結構狀態圖。第6圖為本發明於旋轉單元旋轉後之結構狀態圖。第7圖為本發明於滴入檢側液之結構狀態圖。第8圖為本發明顯示反應檢測結果之結構狀態圖。第9圖為本發明於滴入清洗液之結構狀態圖。第10圖為本發明另一顯示反應檢測結果之結構狀態圖。第11圖為本發明之旋轉單元之第二實施例結構示意圖。第12圖為本發明之旋轉單元之第三實施例結構示意圖。第13圖為本發明之步驟流程圖。Fig. 1 is a schematic diagram of the structure of the biochip detection device of the present invention. Figure 2 is an exploded view of the structure of Figure 1. Fig. 3 is a sectional view of the structure along the line A-A' of Fig. 1. FIG. 4 is a schematic structural diagram of the first embodiment of the rotating unit of the present invention. Fig. 5 is a structural state diagram of the present invention when the liquid to be tested is dropped. Fig. 6 is a structural state diagram of the present invention after the rotating unit is rotated. Fig. 7 is a structural state diagram of the present invention when the liquid is dripped into the detection side. Fig. 8 is a structural state diagram showing the reaction detection result of the present invention. Fig. 9 is a structural state diagram of the present invention in which the cleaning solution is dripped. Fig. 10 is another structural state diagram showing the reaction detection result of the present invention. FIG. 11 is a schematic structural diagram of the second embodiment of the rotating unit of the present invention. Fig. 12 is a schematic structural diagram of the third embodiment of the rotating unit of the present invention. Fig. 13 is a flow chart of the steps of the present invention.

100:生物晶片檢測裝置 100: Biochip detection device

10:基材 10: Substrate

11:第一樞接孔 11: The first pivot hole

21:反應層 21: Reactive Layer

22:吸液層 22: Absorbent layer

221:吸液開孔 221: suction opening

222:吸液區域 222: suction area

23:固定層 23: Fixed layer

231:反應開孔 231: reaction opening

232:第二樞接孔 232: Second pivot hole

30:旋轉單元 30: Rotary unit

31:本體 31: Ontology

32:樞軸件 32: Pivot piece

321:旋轉自由端 321: Rotating free end

322:固定端 322: Fixed end

33:滴入孔 33: Drip hole

35:第三樞接孔 35: The third pivot hole

Claims

一種生物晶片檢測裝置，其包括：一基材(10)；一反應模組(20)，其貼合該基材(10)上，該反應模組(20)包括由下至上層疊的一反應層(21)、一吸液層(22)與一固定層(23)，該反應層(21)鄰近該基材(10)，該吸液層(22)具有一吸液開孔(221)與位於該吸液開孔(221)周邊的一吸液區域(222)，該固定層(23)具有一反應開孔(231)，該反應開孔(231)與該吸液開孔(221)形成相連通；以及一旋轉單元(30)，設置於該反應模組(20)上，該旋轉單元(30)具有一本體(31)、位於該本體(31)上之一樞軸件(32)及複數個滴入孔(33)，該樞軸件(32)連接該反應模組(20)與該基材(10)，該旋轉單元(30)利用該樞軸件(32)於該反應模組(20)上旋轉作動，該些滴入孔(33)的位置對應該反應開孔(231)，且彼此相連通該反應層(21)。 A biological chip detection device, comprising: a substrate (10); a reaction module (20), which is attached to the substrate (10), the reaction module (20) including a reaction module stacked from bottom to top layer (21), a liquid absorption layer (22) and a fixed layer (23), the reaction layer (21) is adjacent to the substrate (10), the liquid absorption layer (22) has a liquid absorption opening (221) With a liquid suction area (222) around the liquid suction opening (221), the fixed layer (23) has a reaction opening (231), the reaction opening (231) and the liquid suction opening (221) ) to form a communication; and a rotating unit (30) arranged on the reaction module (20), the rotating unit (30) having a body (31), a pivot member (31) on the body (31) 32) and a plurality of dropping holes (33), the pivot member (32) connects the reaction module (20) and the base material (10), the rotation unit (30) uses the pivot member (32) to The reaction module (20) rotates upward, and the positions of the dripping holes (33) correspond to the reaction openings (231), and are connected to each other with the reaction layer (21).

如請求項1所述之生物晶片檢測裝置，其中該些滴入孔(33)之側邊分別具有不同的一滴注標記(34)。 The biochip detection device according to claim 1, wherein the sides of the dripping holes (33) are respectively provided with a different dripping mark (34).

如請求項1所述之生物晶片檢測裝置，其中該本體(31)具有一滴入區域(F)，該些滴入孔(33)位於該滴入區域(F)內，並以該滴入區域(F)的中心位置向外呈間隔環繞設置。 The biochip detection device as claimed in claim 1, wherein the body (31) has a drop-in area (F), the drop-in holes (33) are located in the drop-in area (F), and the drop-in area (F) The center position of the area (F) is arranged around the center at intervals.

如請求項1所述之生物晶片檢測裝置，其中該本體(31)具有一滴入區域(F)，該些滴入孔(33)位於該滴入區域(F)內，並以該滴入區域(F)的中心位置向外呈間隔環繞設置且該些滴入孔(33)彼此交錯間隔設置。 The biochip detection device as claimed in claim 1, wherein the body (31) has a drop-in area (F), the drop-in holes (33) are located in the drop-in area (F), and the drop-in area (F) The central position of the area (F) is arranged around the outside at intervals, and the dripping holes (33) are staggered and arranged at intervals.

如請求項1所述之生物晶片檢測裝置，其中該本體(31)具有一滴入區域(F)，該些滴入孔(33)位於該滴入區域(F)內，該些滴入孔(33)係環繞設置為至少兩個環繞區域，區分為一第一環繞區域(S1)與一第二環繞區域(S2)，位於該第一環繞區域(S1)與該第二環繞區域(S2)的該些滴入孔(33)彼此交錯設置。 The biochip detection device according to claim 1, wherein the body (31) has a drop-in area (F), the drop-in holes (33) are located in the drop-in area (F), and the drop-in holes (33) The system surround is set to At least two surrounding areas, divided into a first surrounding area (S1) and a second surrounding area (S2), the drip holes located in the first surrounding area (S1) and the second surrounding area (S2) (33) are staggered with each other.

如請求項1所述之生物晶片檢測裝置，其中該反應開孔(231)的孔徑大於該吸液開孔(221)的孔徑，且該吸液區域(222)部分裸露於該反應開孔(231)。 The biochip detection device according to claim 1, wherein the diameter of the reaction opening (231) is larger than the diameter of the liquid suction opening (221), and the liquid suction region (222) is partially exposed to the reaction opening ( 231).

如請求項1所述之生物晶片檢測裝置，其中該反應層(21)的尺寸小於該吸液層(22)的尺寸，該反應層(21)抵觸該吸液開孔(221)與局部該吸液區域(222)。 The biochip inspection device according to claim 1, wherein the size of the reaction layer (21) is smaller than the size of the liquid absorption layer (22), and the reaction layer (21) interferes with the liquid absorption opening (221) and partially the liquid absorption layer (221). Suction area (222).

如請求項1所述之生物晶片檢測裝置，其中該樞軸件(32)具有一旋轉自由端(321)與一固定端(322)，該固定端(322)用以固定該基材(10)的底面，該旋轉自由端(321)位於該旋轉單元(30)之該本體(31)上。 The biochip detection device according to claim 1, wherein the pivot member (32) has a free rotating end (321) and a fixed end (322), and the fixed end (322) is used to fix the substrate (10). ), the free rotating end (321) is located on the main body (31) of the rotating unit (30).

如請求項1所述之生物晶片檢測裝置，其中該基材(10)具有一第一樞接孔(11)，該反應模組(20)之該固定層(23)具有一第二樞接孔(232)，該旋轉單元(30)之該本體(31)具有一第三樞接孔(35)，該樞軸件(32)係貫穿樞接於該第一樞接孔(11)、該第二樞接孔(232)與該第三樞接孔(35)。 The biochip detection device according to claim 1, wherein the substrate (10) has a first pivot hole (11), and the fixed layer (23) of the reaction module (20) has a second pivot hole A hole (232), the body (31) of the rotating unit (30) has a third pivot hole (35), the pivot member (32) is pivoted through the first pivot hole (11), The second pivot hole (232) and the third pivot hole (35).

一種生物晶片檢測裝置，其包括：一基材(10)；一反應模組(20)，其貼合該基材(10)上，該反應模組(20)包括由下至上層疊的一反應層(21)、一吸液層(22)與一固定層(23)，該反應層(21)鄰近該基材(10)，該吸液層(22)具有一吸液開孔(221)與位於該吸液開孔(221)周邊的一吸液區域(222)，該固定層(23)具有一反應開孔(231)，該反應開孔(231)與該吸液開孔(221)形成相連通；以及一旋轉單元(30)，設置於該反應模組(20)上，該旋轉單元(30)具有一本體(31)、位於該本體(31)上之一樞軸件(32)及複數個滴入孔(33)，該樞軸件 (32)連接該反應模組(20)與該基材(10)，該旋轉單元(30)利用該樞軸件(32)於該反應模組(20)上旋轉作動，該些滴入孔(33)的位置對應該反應開孔(231)，且彼此相連通；其中，該本體(31)具有一滴入區域(F)，該些滴入孔(33)位於該滴入區域(F)內，並以該滴入區域(F)的中心位置向外呈間隔環繞設置，每一該滴入孔(33)係供一待測液(40)滴入並滲透經該反應開孔(231)、該吸液開孔(221)至該反應層(21)形成一待測區域，於該本體(31)藉由該樞軸件(32)旋轉遠離該反應模組(20)時，該反應開孔(231)與該吸液開孔(221)係供一檢測液(50)滴入至圍繞該待測區域的中心位置，藉由該吸液區域(222)將該檢測液(50)吸附朝向周圍擴散流動至該待測區域以彼此形成反應，並取得最終檢測結果。 A biological chip detection device, comprising: a substrate (10); a reaction module (20), which is attached to the substrate (10), the reaction module (20) including a reaction module stacked from bottom to top layer (21), a liquid absorption layer (22) and a fixed layer (23), the reaction layer (21) is adjacent to the substrate (10), the liquid absorption layer (22) has a liquid absorption opening (221) With a liquid suction area (222) around the liquid suction opening (221), the fixed layer (23) has a reaction opening (231), the reaction opening (231) and the liquid suction opening (221) ) to form a communication; and a rotating unit (30) arranged on the reaction module (20), the rotating unit (30) having a body (31), a pivot member (31) on the body (31) 32) and a plurality of drip holes (33), the pivot (32) connecting the reaction module (20) and the base material (10), the rotating unit (30) uses the pivot member (32) to rotate on the reaction module (20), the dripping holes The positions of (33) correspond to the reaction openings (231), and are communicated with each other; wherein, the body (31) has a drop-in area (F), and the drop-in holes (33) are located in the drop-in area (F). ), and the center position of the drop region (F) is arranged in a spaced manner outward, and each drop hole (33) is for a liquid to be tested (40) to be dropped and penetrated through the reaction opening (33). 231), the liquid suction opening (221) to the reaction layer (21) to form an area to be measured, when the main body (31) is rotated away from the reaction module (20) by the pivot member (32), The reaction opening (231) and the liquid suction opening (221) are for dripping a detection solution (50) to a central position around the area to be measured, and the detection solution (50) is dripped through the suction area (222). 50) Adsorption diffuses toward the surrounding area and flows to the area to be tested to form a reaction with each other, and obtain the final detection result.

如請求項10所述之生物晶片檢測裝置，其中該檢測液(50)包含反應抗體與膠體金。 The biochip detection device according to claim 10, wherein the detection solution (50) contains reactive antibodies and colloidal gold.

如請求項10所述之生物晶片檢測裝置，其中該待測液(40)之容量介於0.1微克至0.2微克。 The biochip testing device according to claim 10, wherein the volume of the liquid to be tested (40) ranges from 0.1 microgram to 0.2 microgram.

一種生物晶片檢測方法，包括：將一基材(10)、一反應模組(20)以及一旋轉單元(30)由下至上設置，且該基材(10)與該反應模組(20)為相貼合固定；於該旋轉單元(30)之複數個滴入孔(33)中的至少一個該滴入孔(33)滴入一待測液(40)，該待測液(40)滲透該反應模組(20)以形成一待測區域，其中，該旋轉單元(30)具有一滴入區域(F)，該些滴入孔(33)位於該滴入區域(F)內，並以該滴入區域(F)的中心位置向外呈間隔環繞設置；旋轉該旋轉單元(30)遠離該反應模組(20)與該基材(10)；於圍繞該待測區域的中心位置滴入一檢測液(50)，使該檢測液(50)與該待測液(40)彼此反應以產生一初次檢測結果；以及於該待測區域滴入一清洗液(60)，其濾除該初次檢測結果中的雜質，據以取得一最終檢測結果。 A biological chip detection method, comprising: arranging a substrate (10), a reaction module (20) and a rotation unit (30) from bottom to top, and the substrate (10) and the reaction module (20) In order to fit and fix; in at least one of the plurality of dripping holes (33) of the rotating unit (30), a liquid to be tested (40) is dropped into the dripping hole (33), and the liquid to be tested (40) Infiltrate the reaction module (20) to form an area to be tested, wherein the rotating unit (30) has a dripping area (F), and the dripping holes (33) are located in the dripping area (F), And the center position of the drop-in area (F) is arranged in an outer circle at intervals; the rotating unit (30) is rotated away from the reaction module (20) and the base material (10); around the center of the area to be tested A detection liquid (50) is dropped into the position, so that the detection liquid (50) and the liquid to be tested (40) react with each other to generate an initial detection result; and A cleaning solution (60) is dropped into the area to be tested, and the impurities in the initial test result are filtered out, so as to obtain a final test result.

如請求項13所述之生物晶片檢測方法，其中於滴入該待測液(40)的步驟之後，更包括一乾燥步驟，該乾燥時間介於5-15分鐘。 The biochip detection method according to claim 13, further comprising a drying step after the step of dropping the liquid to be tested (40), and the drying time is between 5-15 minutes.

如請求項13所述之生物晶片檢測方法，其中於滴入該檢測液(50)的步驟之後，更包括一乾燥步驟，該乾燥時間介於5-15分鐘。 The biochip detection method according to claim 13, further comprising a drying step after the step of dropping the detection liquid (50), and the drying time is between 5-15 minutes.

如請求項13所述之生物晶片檢測方法，其中該檢測液(50)包含反應抗體與膠體金。 The biochip detection method according to claim 13, wherein the detection solution (50) comprises reactive antibodies and colloidal gold.

如請求項13所述之生物晶片檢測方法，其中該清洗液(60)係為純水。 The biological wafer detection method according to claim 13, wherein the cleaning liquid (60) is pure water.

如請求項13所述之生物晶片檢測方法，其中該待測液(40)之容量介於0.1微克至0.2微克。 The biochip detection method according to claim 13, wherein the volume of the liquid to be tested (40) is between 0.1 micrograms and 0.2 micrograms.

如請求項13所述之生物晶片檢測方法，其中該些滴入孔(33)之側邊分別具有不同的一滴注標記(34)，該滴注標記(34)係對應該待測液(40)的一檢測資料。 The biochip detection method according to claim 13, wherein the sides of the dripping holes (33) are respectively provided with a different dripping mark (34), and the dripping mark (34) corresponds to the liquid to be tested ( 40) of a test data.

如請求項19所述之生物晶片檢測方法，其中該檢測資料包含受測者資料、檢體及檢測項目。 The biochip detection method as claimed in claim 19, wherein the detection data includes subject information, specimens and detection items.

如請求項13所述之生物晶片檢測方法，其中該旋轉單元(30)係利用一樞軸件(32)進行旋轉遠離該反應模組(20)與該基材(10)。 The biochip detection method according to claim 13, wherein the rotating unit (30) uses a pivot member (32) to rotate away from the reaction module (20) and the substrate (10).

如請求項13所述之生物晶片檢測方法，其中該旋轉單元(30)係由透明材料所製成的透明基材。 The biochip detection method according to claim 13, wherein the rotating unit (30) is a transparent substrate made of a transparent material.