TWI754618B - Skin external composition for anti-aging containing germinated glycine gracilis extract - Google Patents
Skin external composition for anti-aging containing germinated glycine gracilis extract Download PDFInfo
- Publication number
- TWI754618B TWI754618B TW105130366A TW105130366A TWI754618B TW I754618 B TWI754618 B TW I754618B TW 105130366 A TW105130366 A TW 105130366A TW 105130366 A TW105130366 A TW 105130366A TW I754618 B TWI754618 B TW I754618B
- Authority
- TW
- Taiwan
- Prior art keywords
- bean
- extract
- sprouted
- above step
- cinnamon
- Prior art date
Links
Images
Landscapes
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
本發明涉及一種含有發芽寬葉蔓豆萃取物作為有效成分的抗老化皮膚外用劑組合物，更具體地，涉及一種藉由含有在添加了肉桂酸（cinnamic acid）的培養液中發芽的寬葉蔓豆萃取物而具有優異的抗老化效果的皮膚外用劑組合物。在使用本發明的皮膚外用劑組合物的情況下，能夠使合成膠原蛋白的真皮纖維母細胞的表現增加，藉由抑制膠原蛋白分解酶（collagenase）的表現及活性，從而具有使皮膚彈性得到強化、皺紋得到改善等優異的抗老化效果。The present invention relates to an anti-aging skin external preparation composition containing a sprouted cinnamon bean extract as an active ingredient, and more particularly, to an anti-aging skin rinsing bean extract germinated in a culture solution supplemented with cinnamic acid (cinnamic acid). And a skin external preparation composition with excellent anti-aging effect. When the skin external preparation composition of the present invention is used, the expression of dermal fibroblasts that synthesize collagen can be increased, and the expression and activity of collagenase can be inhibited, thereby enhancing skin elasticity. , wrinkles are improved and other excellent anti-aging effects.
Description
本發明涉及一種含有發芽寬葉蔓豆萃取物作為有效成分的抗老化皮膚外用劑組合物，更具體地，涉及一種藉由含有在添加了肉桂酸（cinnamic acid）的培養液中發芽的寬葉蔓豆萃取物而具有優異的抗老化效果的皮膚外用劑組合物。The present invention relates to an anti-aging skin external preparation composition containing a sprouted cinnamon bean extract as an active ingredient, and more particularly, to an anti-aging skin rinsing bean extract germinated in a culture solution supplemented with cinnamic acid (cinnamic acid). And a skin external preparation composition with excellent anti-aging effect.
皮膚作為人體的第一道防護膜，對體內的器官進行保護，使其免受溫度及濕度的變化和紫外線，以及公害物質等外部環境的刺激，對於體溫調節等維持活體恒定性也起到重要的作用。但是，外部帶來的過度的物理刺激和化學刺激，以及壓力、營養缺乏等會降低皮膚正常的功能，還會促進彈性損失、角質化、產生皺紋等皮膚老化現象。As the first protective film of the human body, the skin protects the organs in the body from changes in temperature and humidity, ultraviolet rays, and external environmental stimuli such as polluting substances. effect. However, excessive physical and chemical stimulation from the outside, as well as stress and nutritional deficiencies, will reduce the normal function of the skin, and will also promote skin aging such as loss of elasticity, keratinization, and wrinkles.
為了防止這種現象，並維持更加健康且有彈性的皮膚，人們正在藉由製備含有從各種植物、微生物等中獲得的生理活性物質的化妝品來維持皮膚的固有功能，並使皮膚細胞得到活化，從而有效地抑制皮膚老化而做出努力。但是，現有的化妝品原料大部分具有功效不足，並且會誘發皮膚副作用等的多種問題。In order to prevent this phenomenon and maintain healthier and more elastic skin, people are preparing cosmetics containing physiologically active substances obtained from various plants, microorganisms, etc. to maintain the inherent functions of the skin and activate skin cells, Thereby, efforts are made to effectively inhibit skin aging. However, most of the existing cosmetic raw materials have insufficient efficacy and induce various problems such as skin side effects.
本發明中所使用的寬葉蔓豆（Glycine gracilis）的名稱源於其扁扁的形態，還被稱為半野生大豆、穭豆（青松）、寬葉蔓豆（迎日）、蔓豆（京畿道）等。與最為常見的大豆不同，寬葉蔓豆為橢圓形，並且具有扁扁的細長的形態。在初伏之前種植半野生大豆時，會長出很多的藤，但如果在初伏之後種植時，長出的半野生大豆的個頭小，並且在每株植物底下會一次性地結出很多豆子，以至於連將腳伸進去的空隙也沒有。半野生大豆的葉子較窄且偏小，會開出藍色的花。將半野生大豆養成豆芽時，具有生長快、頭部細長且又小又軟、味道好的特點。The name of Glycine gracilis used in the present invention is derived from its flattened form, and it is also called semi-wild soybean, Glycine gracilis (green pine), Glycine gracilis (Yingri), Glycine gracilis (Gyeonggi-do), etc. . Unlike the most common soybean, the broadleaf bean is oval in shape and has a flat, elongated shape. When planting semi-wild soybeans before the first bloom, many vines will grow, but if planted after the first bloom, the semi-wild soybeans that grow are small in size and will produce many beans at once under each plant, So much so that there is no space to put your feet in. Semi-wild soybeans have narrower, smaller leaves and blue flowers. When semi-wild soybeans are grown into sprouts, they have the characteristics of fast growth, slender heads, small and soft, and good taste.
豆中含有豐富的優異的抗氧化成分及抗老化成分，考慮到這一特徵，本發明人在韓國公開專利第2013-0107123號中公開了一種含有未發芽的半野生大豆（寬葉蔓豆）的萃取物的組合物，並指出這種組合物具有優異的抗氧化、抗老化、美白或保濕效果。Beans are rich in excellent antioxidant and anti-aging components, and in consideration of this feature, the present inventors disclosed in Korean Laid-Open Patent No. 2013-0107123 an extract containing ungerminated semi-wild soybeans (Cannabis vulgaris) The composition of the composition, and pointed out that this composition has excellent antioxidant, anti-aging, whitening or moisturizing effect.
在與這種寬葉蔓豆萃取物的老化防止效果相關的研究的延伸方面，本發明人為了更加強化寬葉蔓豆萃取物的皮膚皺紋改善效果及皮膚彈性增強效果，進行努力研究的結果，確認了發芽的寬葉蔓豆萃取物，尤其是在添加了肉桂酸的培養液中發芽的寬葉蔓豆萃取物與未發芽的寬葉蔓豆或在沒有肉桂酸的情況下發芽的寬葉蔓豆萃取物相比，能夠更加增強真皮纖維母細胞生長因數的表現，進一步抑制膠原蛋白分解酶的表現，顯示出優異的皮膚老化抑制效果，從而完成了本發明。As an extension of the research related to the anti-aging effect of such a broadleaf bean extract, the present inventors have conducted diligent studies in order to further strengthen the skin wrinkle improvement effect and skin elasticity enhancement effect of the broadleaf bean extract, and have confirmed that sprouted Cinnamon bean extract, especially when sprouted in broth supplemented with cinnamic acid, strengthens dermal fibers more than either unsprouted or sprouted Cinnamon bean extract without cinnamic acid The expression of the blast growth factor and the further inhibition of the expression of the collagen-degrading enzyme show an excellent skin aging inhibitory effect, thereby completing the present invention.
現有技術文獻 專利文獻 1. 韓國公開專利第2013-0107123號（2013.10.01）Prior Art Documents Patent Documents 1. Korean Laid-Open Patent Publication No. 2013-0107123 (2013.10.01)
所欲解決的技術問題The technical problem to be solved
本發明的目的在於，提供一種藉由含有在添加了肉桂酸的培養液中發芽的寬葉蔓豆的萃取物作為有效成分，從而顯示出優異的抗老化效果的皮膚外用劑組合物。An object of the present invention is to provide a skin external preparation composition which exhibits an excellent anti-aging effect by containing, as an active ingredient, an extract of C.
技術手段technical means
為了實現上述目的，本發明提供一種含有發芽寬葉蔓豆萃取物作為有效成分的抗老化皮膚外用劑組合物，其特徵為，所述發芽寬葉蔓豆萃取物是從在添加了肉桂酸的培養液中發芽的寬葉蔓豆中獲得的。In order to achieve the above object, the present invention provides an anti-aging skin external preparation composition containing a sprouted cinnamon bean extract as an active ingredient, characterized in that the sprouted cinnamon bean extract is germinated from a culture solution to which cinnamic acid has been added. of broadleaf cranberries.
此外，本發明提供一種含有發芽寬葉蔓豆萃取物作為有效成分的皮膚皺紋改善用皮膚外用劑組合物，其特徵為，所述發芽寬葉蔓豆萃取物是從在添加了肉桂酸的培養液中發芽的寬葉蔓豆中獲得的。In addition, the present invention provides a skin wrinkle-improving skin external preparation composition containing a sprouted cinnamon bean extract as an active ingredient, characterized in that the sprouted cinnamon bean extract is germinated from a culture solution to which cinnamic acid has been added. Obtained from broadleaf cranberry.
此外，本發明提供一種含有發芽寬葉蔓豆萃取物作為有效成分的皮膚彈性改善用皮膚外用劑組合物，其特徵為，所述發芽寬葉蔓豆萃取物是從在添加了肉桂酸的培養液中發芽的寬葉蔓豆中獲得的。Furthermore, the present invention provides an external preparation for skin for improving skin elasticity comprising a sprouted cinnamon bean extract as an active ingredient, characterized in that the sprouted cinnamon bean extract is germinated from a culture solution to which cinnamic acid has been added. Obtained from broadleaf cranberry.
此外，本發明提供一種在皮膚外用劑組合物（具體為化妝品組合物或藥學組合物）的製備中，發芽寬葉蔓豆萃取物作為皮膚皺紋改善劑的用途。In addition, the present invention provides a use of the sprouted Cinnamon bean extract as a skin wrinkle improving agent in the preparation of a skin external preparation composition (specifically, a cosmetic composition or a pharmaceutical composition).
此外，本發明提供一種在皮膚外用劑組合物（具體為化妝品組合物或藥學組合物）的製備中，發芽寬葉蔓豆萃取物作為皮膚彈性改善劑的用途。In addition, the present invention provides a use of a sprout bean extract as a skin elasticity improving agent in the preparation of a skin external preparation composition (specifically, a cosmetic composition or a pharmaceutical composition).
技術效果technical effect
在使用根據本發明的皮膚外用劑組合物的情況下，能夠使合成膠原蛋白的真皮纖維母細胞的表現增加，藉由抑制膠原蛋白分解酶（collagenase）的表現及活性，從而具有使皮膚彈性得到強化、皺紋得到改善等優異的抗老化效果。When the skin external preparation composition according to the present invention is used, the expression of dermal fibroblasts that synthesize collagen can be increased, and the expression and activity of collagenase can be inhibited, thereby improving skin elasticity. Excellent anti-aging effects such as strengthening and wrinkle improvement.
在未以其它方式定義的情況下，本說明書中所使用的所有技術術語及科學術語具有與本領域熟練的技術人員通常理解的意思是相同的。通常，本說明書中使用的命名法為本領域中熟知且通常使用的命名法。Unless otherwise defined, all technical and scientific terms used in this specification have the same meaning as commonly understood by those skilled in the art. In general, the nomenclature used in this specification is one that is well known and commonly used in the art.
本發明中使用的“肉桂酸發芽寬葉蔓豆萃取物”是指從在添加了肉桂酸的培養液中發芽的寬葉扁豆中萃取出的物質，沒有前綴詞的“肉桂酸”的“發芽寬葉蔓豆萃取物”是指在未添加肉桂酸的通常的培養液中發芽的寬葉蔓豆中萃取出的物質。As used in the present invention, "cinnamic acid sprouted broadleaf lentils extract" refers to a substance extracted from broadleaf lentils germinated in a culture solution to which cinnamic acid is added, and "sprouted broadleaf lentils" without the prefix "cinnamic acid" The term "extract" refers to a substance extracted from Lentil bean sprouts germinated in a normal culture solution to which no cinnamic acid has been added.
此外，在本發明中“膠原蛋白分解酶”和“膠原酶（collagenase）”作為相同的意思使用。In addition, in this invention, "collagenase" and "collagenase" are used as the same meaning.
在本發明的實施例中，對從在肉桂酸水溶液中發芽的寬葉蔓豆中獲得的萃取物、從在沒有肉桂酸水溶液的情況下發芽的寬葉蔓豆中獲得萃取物，以及從未發芽的寬葉蔓豆中獲得的萃取物的真皮纖維母細胞生長因數及膠原蛋白分解酶的表現分別進行測定而比較的結果，可知在肉桂酸水溶液中發芽的寬葉蔓豆萃取物能夠更加促進真皮纖維母細胞生長因數的表現，能夠更加抑制膠原蛋白分解酶的表現和活性，從而顯示出優異的皮膚皺紋改善效果及皮膚彈性增強效果。In the examples of the present invention, extracts obtained from broadleaf spearmint sprouted in an aqueous cinnamic acid solution, extracts from broad leaf sprouts sprouted in the absence of an aqueous cinnamic acid solution, and unsprouted broad leaf sprouts The dermal fibroblast growth factor and the expression of collagenase were measured and compared with the extracts obtained from , and it was found that the fenugreek bean extract germinated in a cinnamic acid aqueous solution can further promote the expression of dermal fibroblast growth factor. , which can further inhibit the expression and activity of collagen-degrading enzymes, thereby showing excellent skin wrinkle improvement effect and skin elasticity enhancement effect.
此外，本發明的一個觀點為，涉及一種含有發芽寬葉蔓豆萃取物作為有效成分的抗老化皮膚外用劑組合物，其特徵為，所述發芽寬葉蔓豆萃取物是從在添加了肉桂酸的培養液中發芽的寬葉蔓豆中獲得的。In addition, one aspect of the present invention relates to an anti-aging skin external preparation composition containing as an active ingredient a sprouted cinnamon bean extract, characterized in that the sprouted cinnamon bean extract is obtained from a culture solution to which cinnamic acid has been added. Obtained from sprouted broadleaf cranberries.
此外，本發明的另一觀點為，涉及一種含有發芽寬葉蔓豆萃取物作為有效成分的皮膚皺紋改善用或皮膚彈性改善用皮膚外用劑組合物，其特徵為，所述發芽寬葉蔓豆萃取物是從在添加了肉桂酸的培養液中發芽的寬葉蔓豆中獲得的。In addition, another aspect of the present invention relates to a skin wrinkle-improving or skin elasticity-improving skin external preparation composition containing a sprouted cinnamon bean extract as an active ingredient, wherein the sprouted cinnamon bean extract is derived from Obtained from broadleaf bean sprouts in a broth supplemented with cinnamic acid.
在本發明中，所述組合物藉由促進合成皮膚組織的主要結構成分的膠原蛋白的真皮纖維母細胞生長因數的表現或藉由抑制膠原蛋白分解酶的表現來顯示出抗老化效果、尤其是皮膚彈性改善效果及皮膚皺紋改善效果作為特徵。In the present invention, the composition exhibits an anti-aging effect by promoting the expression of dermal fibroblast growth factor or by inhibiting the expression of collagen degrading enzymes by promoting the synthesis of collagen, which is the main structural component of skin tissue, especially The skin elasticity improvement effect and the skin wrinkle improvement effect are characterized.
本發明中使用的寬葉蔓豆的學名為Glycine gracilis，我國將其稱為半野生大豆、半野生豆、穭豆、寬葉蔓豆、蔓豆等多種。“發芽”為植物的種子、孢子、花粉及枝或根等上長出的芽開始產生或生長的現象，“發芽寬葉蔓豆”是指開始產生芽的寬葉蔓豆。在本發明中，當寬葉蔓豆的芽生長至20~60mm左右的情況下，將其定義為“發芽寬葉蔓豆”。The scientific name of Glycine gracilis used in the present invention is Glycine gracilis, which is called semi-wild soybean, semi-wild bean, lentil bean, Glycine bean, Glycine bean and the like in China. "Sprouting" refers to the phenomenon in which buds grown on the seeds, spores, pollen, branches or roots of plants begin to produce or grow. In the present invention, when the sprouts of the broadleaf bean grow to about 20 to 60 mm, it is defined as "sprouted broadleaf bean."
此外，本發明中在使寬葉蔓豆發芽時所使用的肉桂酸（cinnamic acid）也被稱為桂酸、桂皮酸、β-苯丙烯酸等，是具代表性的芳香族不飽和羧酸中的一種。大量包含在肉桂油（桂皮油）、秘魯香脂（Peru balsam）、蘇合香油等的植物萃取物中，也可以藉由人工方式合成。本發明中添加到培養液中的肉桂酸具有與植物性激素類似的功能，因此被推測為在植物體內誘導訊號體系（signaling）的啟動，從而生成更多的抗老化相關物質。雖然將肉桂酸添加到培養液中最為佳，但也可以使用茉莉酸（jasmonic acid）或p-香豆酸（p-coumaric acid）來代替肉桂酸。In addition, the cinnamic acid (cinnamic acid) used when germinating the broadleaf bean in the present invention is also referred to as cinnamic acid, cinnamic acid, β-phenylacrylic acid, etc., and is one of representative aromatic unsaturated carboxylic acids . A large amount is contained in plant extracts such as cinnamon oil (cassia oil), Peru balsam, styrax oil, etc. It can also be synthesized artificially. The cinnamic acid added to the culture medium in the present invention has a function similar to that of phytohormone, so it is presumed to induce the activation of the signaling system in the plant, thereby generating more anti-aging related substances. Although it is best to add cinnamic acid to the culture medium, jasmonic acid or p-coumaric acid can also be used in place of cinnamic acid.
本發明中在寬葉蔓豆的發芽中使用的添加了肉桂酸的培養液可以表示肉桂酸水溶液，也可以表示進一步添加了通常培養液中可以添加的輔助成分的肉桂酸水溶液。添加了肉桂酸的培養液中，肉桂酸的濃度優選可以為0.50~1.00mM，更優選可以為0.75mM。The cinnamic acid-added culture solution used in the present invention for germination of broadleaf bean may be an aqueous cinnamic acid solution or an aqueous cinnamic acid solution to which auxiliary components that can be added to a normal culture solution are further added. In the culture solution to which cinnamic acid is added, the concentration of cinnamic acid may preferably be 0.50 to 1.00 mM, and more preferably 0.75 mM.
在本發明中，所述發芽寬葉蔓豆萃取物的特徵為，其可以藉由包括下述步驟的工序製備：In the present invention, the germinated broadleaf bean extract is characterized in that it can be prepared by a process comprising the following steps:
a）利用添加了肉桂酸的培養液使寬葉蔓豆發芽；a) Germination of broadleaf cranberries using a culture medium supplemented with cinnamic acid;
b）使在上述步驟a）中發芽的寬葉蔓豆乾燥後，進行粉末化；b) powdering after drying the broadleaf bean sprouts germinated in the above step a);
c）將在上述步驟b）中粉末化的寬葉蔓豆粉末與極性溶劑混合後進行蒸煮，從而進行一次萃取；c) The broadleaf mandarin bean powder powdered in the above step b) is mixed with a polar solvent and then cooked, so as to perform an extraction;
d）將在上述步驟c）中獲得的一次萃取物進行過濾；d) filtering the primary extract obtained in the above step c);
e）用低極性或非極性有機溶劑對在上述步驟d）中經過過濾的萃取物進行二次萃取；e) secondary extraction of the extract filtered in step d) above with a low polar or non-polar organic solvent;
f）對在上述步驟e）中獲得的最終萃取物進行減壓濃縮。f) Concentrating the final extract obtained in step e) above under reduced pressure.
就本發明的組合物中含有的肉桂酸發芽寬葉蔓豆萃取物而言，尤其是藉由極性變化來依次萃取而制得的，將肉桂酸發芽寬葉蔓豆萃取物使用無水或含水的低級醇（碳原子數為1~3）、丙酮等極性溶劑，較佳為使用乙醇進行一次萃取後，使用乙酸乙酯、二***、苯、氯仿、己烷等低極性或非極性溶劑，較佳為使用乙酸乙酯進行再萃取（二次萃取），從而使活性成分的含量極大化並去除雜質，可以製備出穩定度得到提高的同時功效被極大化的萃取物。然後，可以將其用於皮膚外用劑中，例如添加到從利用可溶化技術的柔膚化妝水到利用乳化技術的營養化妝水、霜、精華液等中來使用。As far as the cinnamic acid sprouted cinnamon bean extract contained in the composition of the present invention is concerned, it is especially prepared by sequential extraction by changing the polarity. Atomic number is 1~3), acetone and other polar solvents, it is preferred to use ethyl acetate, diethyl ether, benzene, chloroform, hexane and other low polar or non-polar solvents after one extraction with ethanol, preferably acetic acid The ethyl ester is re-extracted (secondary extraction) to maximize the active ingredient content and remove impurities, resulting in an extract with improved stability and maximized efficacy. Then, it can be used in external preparations for skin, for example, by adding it to skin softening lotions using solubilization technology to nourishing lotions, creams, and essences using emulsification technology.
此外，藉由在使用極性溶劑的一次萃取和二次萃取之間進行2次過濾的過程，從而可以有效地精製萃取物。較佳地，一次過濾用200~300目的濾布過濾，並將經過一次過濾的濾液在4~15℃下熟化數天後，用濾紙進行二次過濾。In addition, the extract can be efficiently purified by performing a process of 2 filtration between the primary extraction and the secondary extraction using a polar solvent. Preferably, the primary filtration is filtered with a 200-300 mesh filter cloth, and the filtrate after the primary filtration is aged at 4-15° C. for several days, and then the secondary filtration is performed with filter paper.
此外，本發明的特徵為，相對於組合物總重量，包含0.01~10重量%的所述肉桂酸發芽寬葉蔓豆萃取物。如果萃取物的含量不足0.01重量%，則難以期待其效果，如果超過10重量%，則不會進一步明顯地顯示出效果的增加。In addition, the present invention is characterized in that the cinnamic acid sprouted Cinnamon bean extract is contained in an amount of 0.01 to 10% by weight with respect to the total weight of the composition. If the content of the extract is less than 0.01% by weight, it is difficult to expect the effect, and if it exceeds 10% by weight, the effect will not be significantly increased.
將本發明的皮膚外用劑組合物劑型化為化妝品形態的情況下，可以劑型化為柔膚化妝水、收斂化妝水、營養化妝水、眼霜、營養霜、按摩霜、潔面膏、潔面泡沫、清潔水、粉、精華液或面膜等形態，對其劑型沒有特別的限定。此外，本發明的組合物可以包含脂肪物質、有機溶劑、溶解劑、濃縮劑、凝膠化劑、軟化劑、抗氧化劑、懸浮劑、穩定劑、發泡劑（foaming agent）、芳香劑、表面活性劑、水、離子型或非離子型乳化劑、填充劑、金屬離子掩蔽劑、螯合劑、保存劑、維生素、阻斷劑、潤濕劑、精油、染料、顏料、親水性或親油性活性劑、脂質小囊或在化妝品中通常使用的任意的其它成分等在化妝品學或皮膚科學領域通常使用的輔助劑。所述輔助劑以在化妝品學或皮膚科學領域中通常使用的量導入。此外，為了增加皮膚改善效果，本發明的組合物可以含有皮膚吸收促進物質。When the skin external preparation composition of the present invention is formulated into a cosmetic form, it can be formulated into a skin softening lotion, astringent lotion, nutritional lotion, eye cream, nutritional cream, massage cream, facial cleanser, cleansing foam, cleansing The form of water, powder, essence or mask is not particularly limited. In addition, the composition of the present invention may contain fatty substances, organic solvents, dissolving agents, concentrating agents, gelling agents, softening agents, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surface Active agents, water, ionic or nonionic emulsifiers, fillers, metal ion sequestering agents, chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives Adjuvants commonly used in the field of cosmetics or dermatology, such as agents, lipid vesicles, or any other ingredients commonly used in cosmetics. The adjuvants are introduced in amounts commonly used in the field of cosmetic or dermatology. Furthermore, in order to increase the skin-improving effect, the composition of the present invention may contain a skin absorption promoting substance.
本發明的皮膚外用劑組合物除了上述物質以外，在不影響主要效果的範圍內，較佳為可以包含為主要效果帶來提升效果的其它成分。此外，本發明的皮膚外用劑組合物還可以進一步包含保濕劑、潤膚劑、紫外線吸收劑、防腐劑、殺菌劑、抗氧化劑、pH調節劑、有機及無機顏料、香料、冷卻劑或止汗劑。所述成分的混合量在不影響本發明的目的及效果的範圍內，本領域技術人員可以容易地選定，相對於組合物總重量，其混合量可以為0.01~5重量%，具體為0.01~3重量%。The skin external preparation composition of the present invention may preferably contain other components that bring about a boosting effect to the main effect, in addition to the above-mentioned substances, within a range that does not affect the main effect. In addition, the skin external preparation composition of the present invention may further contain a moisturizing agent, an emollient, an ultraviolet absorber, a preservative, a bactericide, an antioxidant, a pH adjuster, an organic and inorganic pigment, a fragrance, a cooling agent or an antiperspirant agent. The mixing amount of the components is within the range that does not affect the purpose and effect of the present invention, and can be easily selected by those skilled in the art. Relative to the total weight of the composition, the mixing amount can be 0.01 to 5% by weight, specifically 0.01 to 5% by weight. 3% by weight.
具體實施方式detailed description
下面，藉由實施例來更加詳細地說明本發明。這些實施例僅是為了例示本發明，本發明的範圍並不應解釋為被這些實施例所限定，這對於本領域技術人員來說是顯而易見的。因此，本發明的實質性範圍被本發明的申請專利範圍和它們的等位物質所限定。Hereinafter, the present invention will be described in more detail by way of examples. These examples are only for illustrating the present invention, and the scope of the present invention should not be construed as being limited by these examples, which will be obvious to those skilled in the art. Accordingly, the substantial scope of the present invention is defined by the claims of the present invention and their equivalents.
[實施例1] 肉桂酸發芽寬葉蔓豆萃取物的製備[Example 1] Preparation of cinnamic acid sprouted Cinnamon bean extract
利用以0.75mM濃度添加了肉桂酸的水，使100g寬葉蔓豆在維持25℃溫度的發芽機中發芽48小時。經過48小時發芽的寬葉蔓豆的芽的長度平均為25mm。Using water to which cinnamic acid was added at a concentration of 0.75 mM, 100 g of broadleaf bean was germinated in a germination machine maintained at a temperature of 25°C for 48 hours. The sprouts of the broadleaf bean sprouts germinated after 48 hours were on average 25 mm in length.
然後，將發芽的寬葉蔓豆用精緻水清洗並乾燥後，將其粉末化，從而獲得100g的肉桂酸發芽寬葉蔓豆粉。將100g的肉桂酸發芽寬葉蔓豆粉加入到1L的70%乙醇水溶液中，在帶有冷凝器的萃取機中蒸煮12小時並萃取後，用300目的濾布過濾。將由此獲得的濾液在4~15℃下放置7天進行熟化後，用沃特曼2號濾紙再次過濾。將如此獲得的萃取液添加到3L裝分液漏斗中，然後加入1L的乙酸乙酯，藉由搖晃使它們很好地混合後，待完全分離成兩層之後，取上層（乙酸乙酯層）。利用分液漏斗將下層（水層）藉由與上述相同的過程再重複萃取兩次。將在各自的萃取過程中分離的上層均合併後，利用帶有冷凝器的蒸餾裝置以50℃進行減壓濃縮並進行乾燥，從而獲得肉桂酸發芽寬葉蔓豆萃取物（乾燥重量13.54g）。Then, after the germinated cinnamon bean was washed with delicate water and dried, it was powdered to obtain 100 g of cinnamic acid sprouted cinnamon bean powder. Add 100 g of cinnamic acid sprouted broadleaf bean powder to 1 L of 70% ethanol aqueous solution, cook in an extractor with a condenser for 12 hours and extract, then filter with a 300-mesh filter cloth. The filtrate thus obtained was aged at 4 to 15° C. for 7 days, and then filtered again with Waterman No. 2 filter paper. The extract thus obtained was added to a 3L separatory funnel, and then 1L of ethyl acetate was added, and after they were well mixed by shaking, after complete separation into two layers, the upper layer (ethyl acetate layer) was taken. . The lower layer (aqueous layer) was extracted twice more by the same procedure as above using a separatory funnel. The upper layers separated in the respective extraction processes were all combined, concentrated under reduced pressure at 50° C. using a distillation apparatus with a condenser, and dried to obtain a cinnamic acid sprouted cranberry extract (13.54 g of dry weight).
[比較例1] 未發芽的寬葉蔓豆萃取物的製備[Comparative Example 1] Preparation of ungerminated broadleaf bean extract
將未發芽的寬葉蔓豆用精製水清洗並乾燥後，將其粉末化，從而獲得100g的寬葉蔓豆粉。將100g的寬葉蔓豆粉加入到1L的70%乙醇水溶液中，在帶有冷凝器的萃取機中蒸煮12小時並萃取後，用300目的濾布過濾。將由此獲得的濾液在4~15℃下放置7天進行熟化後，用沃特曼2號濾紙再次過濾。將如此獲得的萃取液添加到3L裝分液漏斗中，然後加入1L的乙酸乙酯，藉由搖晃使它們很好地混合後，待完全分離成兩層之後，取上層（乙酸乙酯層）。利用分液漏斗將下層（水層）藉由與上述相同的過程再重複萃取兩次。將在各自的萃取過程中分離的上層均合併後，利用帶有冷凝器的蒸餾裝置以50℃進行減壓濃縮並進行乾燥，從而獲得未發芽的寬葉蔓豆萃取物（乾燥重量15.18g）。After washing and drying the ungerminated cinnamon bean with purified water, it was pulverized to obtain 100 g of cinnamon bean powder. Add 100 g of broadleaf bean powder to 1 L of 70% ethanol aqueous solution, cook in an extractor with a condenser for 12 hours and extract, then filter with a 300-mesh filter cloth. The filtrate thus obtained was aged at 4 to 15° C. for 7 days, and then filtered again with Waterman No. 2 filter paper. The extract thus obtained was added to a 3L separatory funnel, and then 1L of ethyl acetate was added, and after they were well mixed by shaking, after complete separation into two layers, the upper layer (ethyl acetate layer) was taken. . The lower layer (aqueous layer) was extracted twice more by the same procedure as above using a separatory funnel. The upper layers separated in the respective extraction processes were all combined, concentrated under reduced pressure at 50° C. using a distillation apparatus with a condenser, and dried to obtain an ungerminated cinnamon bean extract (15.18 g of dry weight).
[比較例2] 發芽寬葉蔓豆萃取物的製備[Comparative Example 2] Preparation of Germinated Broadleaf Cinnamon Bean Extract
首先，利用一次蒸餾水，將100g的寬葉蔓豆在維持25℃的溫度的發芽機中發芽48小時。經過48小時發芽的寬葉蔓豆的芽的長度平均為55mm。芽的平均長度長於利用肉桂酸水溶液發芽的實施例1的原因在於，被認為是在豆子的情況下，肉桂酸會抑制莖的長度的伸長（請參見http://journals.plos.org/plosone/article?id=10.1371/journal.pone.oo69105_）First, with primary distilled water, 100 g of broadleaf bean sprouts were germinated for 48 hours in a germination machine maintained at a temperature of 25°C. The sprouts of broadleaf bean sprouts germinated for 48 hours averaged 55 mm in length. The reason why the average length of the sprouts is longer than that of Example 1 using an aqueous solution of cinnamic acid is that it is believed that in the case of beans, cinnamic acid inhibits the elongation of the length of the stem (see http://journals.plos.org/plosone /article?id=10.1371/journal.pone.oo69105_)
然後，將發芽的寬葉蔓豆用精製水清洗並乾燥後，將其粉末化，從而獲得100g的發芽寬葉蔓豆粉。將100g的發芽寬葉蔓豆粉加入到1L的70%乙醇水溶液中，在帶有冷凝器的萃取機中蒸煮12小時並萃取後，用300目的濾布過濾。將由此獲得的濾液在4~15℃下放置7天進行熟化後，用沃特曼2號濾紙再次過濾。將這樣獲得的萃取液添加到3L裝分液漏斗中，然後加入1L的乙酸乙酯，藉由搖晃使它們很好地混合後，待完全分離成兩層之後，取上層（乙酸乙酯層）。利用分液漏斗將下層（水層）藉由與上述相同的過程再重複萃取兩次。將在各自的萃取過程中分離的上層均合併後，利用帶有冷凝器的蒸餾裝置以50℃進行減壓濃縮並進行乾燥，從而獲得發芽寬葉蔓豆萃取物（乾燥重量14.16g）。Then, after washing and drying the germinated cinnamon bean with purified water, it was pulverized to obtain 100 g of sprouted cinnamon bean powder. Add 100 g of germinated broadleaf bean powder to 1 L of 70% ethanol aqueous solution, cook in an extractor with a condenser for 12 hours and extract, then filter with a 300-mesh filter cloth. The filtrate thus obtained was aged at 4 to 15° C. for 7 days, and then filtered again with Waterman No. 2 filter paper. The extract thus obtained was added to a 3L separatory funnel, and then 1L of ethyl acetate was added, and after they were well mixed by shaking, after the two layers were completely separated, the upper layer (ethyl acetate layer) was taken. . The lower layer (aqueous layer) was extracted twice more by the same procedure as above using a separatory funnel. The upper layers separated in the respective extraction processes were all combined, concentrated under reduced pressure at 50° C. using a distillation apparatus equipped with a condenser, and dried to obtain a sprout bean extract (14.16 g of dry weight).
[試驗例1] 萃取物成分比較分析[Test Example 1] Comparative analysis of extract components
分別對上述實施例1的肉桂酸發芽寬葉蔓豆萃取物、比較例1的未發芽的寬葉蔓豆萃取物，以及比較例2的發芽寬葉蔓豆萃取物的成分進行分析。將上述萃取物分別溶解到70%乙醇中，製備成10,000ppm溶液後，利用高效液相色譜法（HPLC）（沃特世公司（Waters），2695型號（model））進行了成分分析（沃特世公司（Waters），2996PDA檢測器）。固定相利用了關東化學株式會社（Kanto Chemical）的Mightysil RP-18 GP 250-4.6（5μm）色譜柱，流動相使用了下述表1所示的組成比。The components of the cinnamic acid sprouted cinnamon bean extract of the above-mentioned Example 1, the unsprouted cinnamon bean extract of the comparative example 1, and the germinated cinnamon bean extract of the comparative example 2 were respectively analyzed. Each of the above extracts was dissolved in 70% ethanol to prepare a 10,000 ppm solution, and then the components were analyzed by high performance liquid chromatography (HPLC) (Waters, model 2695) (Waters). Waters, 2996PDA detector). As the stationary phase, a Mightysil RP-18 GP 250-4.6 (5 μm) column from Kanto Chemical Co., Ltd. was used, and the composition ratio shown in Table 1 below was used for the mobile phase.
[表1]
結果如圖1中所示，在肉桂酸發芽寬葉蔓豆萃取物中觀察到了在未發芽的寬葉蔓豆萃取物和發芽寬葉蔓豆萃取物中未觀察到的新的峰（peak），從而可以確認新成分的存在。因此，可以預測因該成分的存在使得肉桂酸發芽寬葉蔓豆萃取物比未發芽的寬葉蔓豆萃取物和發芽寬葉蔓豆萃取物具有優異的抗老化功效。As a result, as shown in FIG. 1 , new peaks, which were not observed in the unsprouted Cinnamon bean extract and the germinated Cinnamon bean extract, were observed in the cinnamic acid germinated Cinnamon bean extract, so that a new component could be confirmed. The presence. Therefore, it can be predicted that the presence of this component makes the cinnamic acid sprouted jasmine bean extract superior to the unsprouted and sprouted fenugreek bean extracts to have an excellent anti-aging effect.
[試驗例2] 真皮纖維母細胞生長因數表現測定實驗[Test Example 2] Dermal Fibroblast Growth Factor Expression Measurement Experiment
將所述實施例1的肉桂酸發芽寬葉蔓豆萃取物、比較例1的未發芽的寬葉蔓豆萃取物，以及比較例2的發芽寬葉蔓豆萃取物各自的真皮纖維母細胞生長因數表現量與含有2.5重量%的胎牛血清的達爾伯克改良伊格爾培養基（DMEM,Dulbecco’s Modified Eagle’s Media）培養基進行比較並測定。The expression levels of the dermal fibroblast growth factor of the cinnamic acid sprouted cinnamon bean extract of Example 1, the ungerminated stalk bean extract of Comparative Example 1, and the germinated cinnamon bean extract of Comparative Example 2 were compared with those containing 2.5 % fetal bovine serum in Dulbecco's Modified Eagle's Media (DMEM, Dulbecco's Modified Eagle's Media) medium was compared and determined.
在裝有含2.5重量%的胎牛血清的DMEM培養基的96孔平板培養器（96-well microtiter plate）中以5,000細胞/孔（well）的方式加入人類纖維母細胞(human fibroblasts)（Cascade Biologics公司）（波特蘭，俄勒岡州，美國），培養至長到90%左右。之後，在無血清DMEM培養基中培養24小時後，分別取50ppm的溶解到無血清DMEM培養基中的上述實施例1的肉桂酸發芽寬葉蔓豆萃取物、比較例1的未發芽的寬葉蔓豆萃取物，以及比較例2的發芽寬葉蔓豆萃取物，並處理24小時。陽性對照組是將無血清DMEM培養基替換為含有2.5重量%的胎牛血清的DMEM培養基後，培養24小時後，取細胞培養液。Human fibroblasts (Cascade Biologics) were added at 5,000 cells/well in a 96-well microtiter plate containing 2.5 wt% fetal bovine serum in DMEM medium. Corporation) (Portland, Oregon, USA), cultivated to about 90% growth. Then, after culturing in serum-free DMEM medium for 24 hours, 50 ppm of the cinnamic acid-sprouted Cinnamon bean extract of Example 1 and the unsprouted Cinnamon bean extract of Comparative Example 1 dissolved in serum-free DMEM medium were taken respectively. and the sprouted broadleaf bean extract of Comparative Example 2, and treated for 24 hours. As a positive control group, the serum-free DMEM medium was replaced with a DMEM medium containing 2.5% by weight of fetal bovine serum, and the cell culture medium was taken after culturing for 24 hours.
然後，利用真皮纖維母細胞生長因數測定儀器（美國R&D系統公司）來測定取得的細胞培養液的真皮纖維母細胞生長因數的表現程度。首先，將取得的細胞培養液放入到均勻地塗布有1次真皮纖維母細胞生長因數抗體的96-孔平板（96-well plate）中，在恒溫槽（36℃）中實施抗原-抗體反應3小時。Then, the degree of expression of dermal fibroblast growth factor in the obtained cell culture medium was measured using a dermal fibroblast growth factor measuring instrument (R&D Systems, Inc., USA). First, the obtained cell culture solution was put into a 96-well plate (96-well plate) uniformly coated with dermal fibroblast growth factor antibody once, and the antigen-antibody reaction was carried out in a constant temperature chamber (36°C). 3 hours.
4小時後，將結合有發色團的真皮纖維母細胞生長因數二次抗體放入到96-孔平板中，再次反應15分鐘。15分鐘後，加入顯色誘發物質在室溫下顯色15分鐘，再加入1M的硫酸終止反應（顯色）時，反應液的顏色會帶有黃色，確認了根據反應的進行程度，顯示出了不同程度的藍色。After 4 hours, the chromophore-conjugated dermal fibroblast growth factor secondary antibody was put into a 96-well plate and reacted again for 15 minutes. After 15 minutes, a color-inducing substance was added to develop the color at room temperature for 15 minutes, and 1M sulfuric acid was added to terminate the reaction (color development), and the color of the reaction solution turned yellow. different shades of blue.
利用吸光光度計在450nm下測定帶有藍色的96-孔平板的吸光度，並藉由下述數學式1計算真皮纖維母細胞生長因數的表現程度。此時，將未用組合物處理的組中取得的細胞培養液的反應吸光度作為對照組。即，將非處理組的真皮纖維母細胞生長因數的表現程度作為100，與此對比，求出利用試驗物質處理的組中的真皮纖維母細胞生長因數的表現程度，其結果如下述表2所示。The absorbance of the 96-well plate with blue color was measured at 450 nm using an absorptiophotometer, and the expression degree of the dermal fibroblast growth factor was calculated by the following mathematical formula 1. At this time, the reaction absorbance of the cell culture liquid obtained in the group not treated with the composition was used as a control group. That is, the expression level of the dermal fibroblast growth factor in the non-treated group was set as 100, and the expression degree of the dermal fibroblast growth factor in the group treated with the test substance was calculated. The results are shown in Table 2 below. Show.
[數學式1][Mathematical formula 1]
真皮纖維母細胞表現程度（%）=物質處理細胞組的吸光度/對照組的吸光度×100The degree of expression of dermal fibroblasts (%) = the absorbance of the substance-treated cell group/the absorbance of the control group × 100
[表2]
如上述表2中所示，可知在體外（in vitro），實施例1的肉桂酸發芽寬葉蔓豆萃取物與比較例1的未發芽的寬葉蔓豆萃取物或比較例2的發芽寬葉蔓豆萃取物相比，能夠更加有效地增加真皮纖維母細胞生長因數的表現。因此，可以確認本發明的萃取物或含有其的組合物能夠顯示出優異的抗老化效果，即，優異的皮膚皺紋改善效果及皮膚彈性改善效果。As shown in the above Table 2, it can be seen that the cinnamic acid germinated fenugreek bean extract of Example 1 and the unsprouted fenugreek bean extract of Comparative Example 1 or the germinated fenugreek bean extract of Comparative Example 2 were in vitro in vitro. It can more effectively increase the expression of dermal fibroblast growth factor. Therefore, it was confirmed that the extract of the present invention or the composition containing the same can exhibit an excellent anti-aging effect, that is, an excellent skin wrinkle improvement effect and skin elasticity improvement effect.
[試驗例3]抗老化能力試驗[Test Example 3] Anti-aging ability test
將所述實施例1的肉桂酸發芽寬葉蔓豆萃取物、比較例1的未發芽的寬葉蔓豆萃取物及比較例2的發芽寬葉蔓豆萃取物各自的膠原酶生成抑制能力與生育酚及表沒食子兒茶素沒食子酸酯（EGCG）進行比較來測定。已知生育酚及EGCG使皮膚的表皮細胞再生，具有防止皮膚老化的功能。The collagenase production inhibitory ability of each of the cinnamic acid germinated fenugreek bean extract of Example 1, the ungerminated fenugreek bean extract of Comparative Example 1, and the germinated fenugreek bean extract of Comparative Example 2 was compared with that of tocopherol and epigalactophore. Elocatechin gallate (EGCG) was compared to the determination. Tocopherol and EGCG are known to regenerate epidermal cells of the skin and have a function of preventing skin aging.
在裝有含2.5重量%的胎牛血清的DMEM培養基的96孔平板培養器中以5,000細胞/孔的方式加入人類纖維母細胞（Cascade Biologics公司）（波特蘭，俄勒岡州，美國），培養至長到90%左右。之後，在無血清DMEM培養基中培養24小時後，對溶解到無血清DMEM培養基中的上述實施例1的肉桂酸發芽寬葉蔓豆萃取物、比較例1的未發芽的寬葉蔓豆萃取物，以及比較例2的發芽寬葉蔓豆萃取物進行處理，使它們的濃度分別達到50ppm，生育酚及EGCG（西格瑪奧德里奇公司）分別以50μM的濃度處理24小時後，取細胞培養液。Human fibroblasts (Cascade Biologics, Inc.) (Portland, OR, USA) were added at 5,000 cells/well in a 96-well plate incubator containing 2.5 wt% fetal bovine serum in DMEM, and cultured. to about 90%. Then, after culturing in serum-free DMEM medium for 24 hours, the cinnamic acid-sprouted C. broadleaf extract of Example 1, the unsprouted C. broadleaf extract of Comparative Example 1, and the comparative example were dissolved in serum-free DMEM medium. 2 were treated with the sprouted Cinnamon bean extract so that their concentrations reached 50 ppm, respectively, tocopherol and EGCG (Sigma-Aldrich) were treated at a concentration of 50 μM for 24 hours, and the cell culture medium was taken.
然後，利用膠原酶測定儀器（美國Amersham Pharmacia公司）來測定取得的細胞培養液的膠原酶生成程度。首先，將取得的細胞培養液放入到均勻地塗布有1次膠原酶抗體的96-孔平板中，在恒溫槽（36℃）中實施抗原-抗體反應3小時。Then, the collagenase production degree of the obtained cell culture medium was measured using a collagenase measuring instrument (Amersham Pharmacia, USA). First, the obtained cell culture medium was put into a 96-well plate uniformly coated with a primary collagenase antibody, and an antigen-antibody reaction was performed in a constant temperature chamber (36° C.) for 3 hours.
3小時後，將結合有發色團的膠原酶二次抗體放入到96-孔平板中，再次反應15分鐘。15分鐘後，加入顯色誘發物質在室溫下顯色15分鐘，再加入1M的硫酸終止反應（顯色）時，反應液的顏色會帶有黃色，確認了根據反應的進行程度，顯示出了不同程度的黃色。After 3 hours, the chromophore-conjugated collagenase secondary antibody was placed in a 96-well plate and reacted again for 15 minutes. After 15 minutes, a color-inducing substance was added to develop the color at room temperature for 15 minutes, and 1M sulfuric acid was added to terminate the reaction (color development), and the color of the reaction solution turned yellow. varying degrees of yellow.
利用吸光光度計在450nm下測定帶有黃色的96-孔平板的吸光度，並藉由下述數學式2計算膠原酶的合成程度。此時，將未用組合物處理的組中取得的細胞培養液的反應吸光度作為對照組。即，將非處理組的膠原酶的表現程度作為100，與此對比，求出利用試驗物質處理的組中的膠原酶的表現程度，其結果如下述表3所示。The absorbance of the yellow 96-well plate was measured at 450 nm with an absorptiophotometer, and the synthesis degree of collagenase was calculated by the following mathematical formula 2. At this time, the reaction absorbance of the cell culture liquid obtained in the group not treated with the composition was used as a control group. That is, the expression degree of collagenase in the group treated with the test substance was calculated in comparison with the expression degree of collagenase in the non-treated group as 100, and the results are shown in Table 3 below.
[數學式2][Mathematical formula 2]
膠原酶表現程度（%）=物質處理細胞組的吸光度/對照組的吸光度×100The degree of collagenase expression (%) = the absorbance of the substance-treated cell group / the absorbance of the control group × 100
[表3]
如上述表3中所示，可知在試管內（in vitro），實施例1的肉桂酸發芽寬葉蔓豆萃取物與比較例1的未發芽的寬葉蔓豆萃取物或比較例2的發芽寬葉蔓豆萃取物相比，能夠更加有效地抑制膠原酶的表現，比已知為抗老化物質的生育酚和EGCG具有更優異的膠原酶表現抑制能力。因此，可以確認本發明的萃取物或含有其的組合物能夠顯示出優異的抗老化效果、尤其是可以顯示出優異的皮膚皺紋改善效果及皮膚彈性改善效果。As shown in Table 3 above, it can be seen that in vitro, the cinnamic acid germinated cranberry extract of Example 1, the unsprouted cranberry extract of Comparative Example 1, or the germinated cranberry extract of Comparative Example 2 In contrast, it can inhibit the expression of collagenase more effectively, and has a superior ability to inhibit the expression of collagenase than tocopherol and EGCG, which are known anti-aging substances. Therefore, it was confirmed that the extract of the present invention or the composition containing the same can exhibit an excellent antiaging effect, especially an excellent skin wrinkle improvement effect and skin elasticity improvement effect.
以上對本發明內容的特定部分進行了詳細的說明，本領域技術人員應知曉，這種具體說明只是較佳的實施方式，本發明的範圍並不能被這些所限定。因此，本發明的實質範圍應被隨附的申請專利範圍和它們的等價物所定義。The specific part of the content of the present invention has been described in detail above, and those skilled in the art should know that this specific description is only a preferred embodiment, and the scope of the present invention is not limited by these. Accordingly, the true scope of the present invention should be defined by the appended claims and their equivalents.
此外，下面對包含本發明的組合物的皮膚外用劑組合物的劑型例進行更加詳細的說明，但應知曉，本發明的範圍並不被這些劑型例所限定。In addition, the dosage form examples of the skin external preparation composition containing the composition of the present invention will be described in more detail below, but it should be understood that the scope of the present invention is not limited by these dosage form examples.
[劑型例1] 營養化妝水[Formulation example 1] Nutritious lotion
根據下述表4中記載的組成採用通常的方法製備營養化妝水（單位：重量%）A nutritional lotion (unit: % by weight) was prepared according to the composition described in the following Table 4 by an ordinary method.
[表4]
[劑型例2] 營養霜[Formulation example 2] Nutrient cream
根據下述表5中記載的組成採用通常的方法製備營養霜（單位：重量%）Nutrient cream (unit: % by weight) was prepared by an ordinary method according to the composition described in the following Table 5
[表5]
[劑型例3] 按摩霜[Formulation example 3] Massage cream
根據下述表6中記載的組成採用通常的方法製備按摩霜（單位：重量%）A massage cream (unit: % by weight) was prepared according to the composition described in the following Table 6 by a usual method
[表6]
[劑型例4] 面膜[Formulation Example 4] Mask
根據下述表7中記載的組成採用通常的方法製備面膜（單位：重量%）A mask (unit: % by weight) was prepared by an ordinary method according to the composition described in the following Table 7
[表7]
[劑型例5] 皮膚外用劑中的軟膏[Formulation Example 5] Ointment in external preparation for skin
根據下述表8中記載的組成採用通常的方法製備軟膏（單位：重量%）An ointment (unit: % by weight) was prepared by an ordinary method according to the composition described in the following Table 8
[表8]
無without
第1圖為示出分別對未發芽的寬葉蔓豆萃取物（比較例1）、發芽寬葉蔓豆萃取物（比較例2）、肉桂酸發芽寬葉蔓豆萃取物（實施例1）進行高效液相色譜法（high performance liquid chromatography，HPLC）成分分析的結果進行比較的圖。Fig. 1 shows the high-performance liquid chromatography of the unsprouted Cinnamon bean extract (Comparative Example 1), the germinated Cinnamon bean extract (Comparative Example 2), and the cinnamic acid germinated Cinnamon bean extract (Example 1), respectively. A graph comparing the results of component analysis (high performance liquid chromatography, HPLC).
Claims (10)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW105130366A TWI754618B (en) | 2016-09-20 | 2016-09-20 | Skin external composition for anti-aging containing germinated glycine gracilis extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW105130366A TWI754618B (en) | 2016-09-20 | 2016-09-20 | Skin external composition for anti-aging containing germinated glycine gracilis extract |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| TW201811356A TW201811356A (en) | 2018-04-01 |
| TWI754618B true TWI754618B (en) | 2022-02-11 |
Family
ID=62639143
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TW105130366A TWI754618B (en) | 2016-09-20 | 2016-09-20 | Skin external composition for anti-aging containing germinated glycine gracilis extract |
Country Status (1)
| Country | Link |
|---|---|
| TW (1) | TWI754618B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040142007A1 (en) * | 2001-06-01 | 2004-07-22 | Philippe Moussou | Cosmetic preparations containing an extract of germinating plants |
-
2016
- 2016-09-20 TW TW105130366A patent/TWI754618B/en active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040142007A1 (en) * | 2001-06-01 | 2004-07-22 | Philippe Moussou | Cosmetic preparations containing an extract of germinating plants |
Also Published As
| Publication number | Publication date |
|---|---|
| TW201811356A (en) | 2018-04-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR102020828B1 (en) | Whitening cosmetics containing the Saururus chinensis (Lour.) Baill. with improved whitening activity by Jeju Shindari fermentation method and producing method thereof | |
| KR20090056521A (en) | Cosmetic composition for skin-aging protection and wrinkle improvement comprising the extract of nephelium lappaceum and litchi chinensis sonn as active ingredient | |
| KR101619571B1 (en) | Anti-aging Composition for Skin External Application Comprising Magnolia Placenta Culture Extracts | |
| KR20070019120A (en) | Anti-Wrinkle Cosmetic Composition Comprising the Extract of Paeonia lactiflora as Active Ingredient | |
| KR101143717B1 (en) | Composition for Skin External Application Comprising Extract of Nelumbo Nucifera Callus | |
| KR100253843B1 (en) | Chestnut inner bark extracts containing skin composition | |
| KR20160000093A (en) | A cosmetic composition for antioxidizing and whitening containing the extracts of germinated crops | |
| TWI754618B (en) | Skin external composition for anti-aging containing germinated glycine gracilis extract | |
| KR20120110453A (en) | Skin external composition containing myrothamnus flabellifolia callus extract | |
| KR102371164B1 (en) | Skin external composition for anti-aging containing germinated Glycine gracilis extract | |
| EP3381441B1 (en) | External use skin composition for antiaging containing germinated glycine gracilis extract as active ingredient | |
| KR101050484B1 (en) | Skin whitening composition containing lactone compound as an active ingredient | |
| KR101776692B1 (en) | Composiitn for skin whitening comprising extract of Osmanthus heterophylla | |
| KR101619710B1 (en) | Anti-aging Composition for Skin External Application Comprising Liriodendron tulipifera Placenta Culture Extracts | |
| KR20190102364A (en) | Anti-aging composition and cosmetic using the same | |
| KR102216210B1 (en) | Solid fermentation composition manufactured by rumex coreanus and vegetable worms and functional cosmetic composition comprising the same | |
| KR20130050173A (en) | A skin external composition containing extract drived from rhizophora mangle | |
| KR101949956B1 (en) | Cosmetic composition for improving skin wrinkle comprising Oxya chinensis sinuosa extract or compound isolated from the extract as effective component | |
| JP6629271B2 (en) | Endo180 production promoter | |
| JP2010024190A (en) | Skin barrier function ameliorator | |
| KR101619711B1 (en) | Anti-aging Composition for Skin External Application Comprising Schizandra chinensis Placenta Culture Extracts | |
| JP4886878B2 (en) | Tyrosinase activity inhibitor and whitening cosmetic | |
| KR101978335B1 (en) | Fragrance composition comprising fermented product by yeast from flowers and manufacturing method thereof | |
| KR102374884B1 (en) | Cosmetic composition containing the extract of dendropanax morbifera and wild ginseng as active ingredient | |
| JP2011068570A (en) | Laminin-5 production promoter, dermal basement membrane normalizing agent and skin damage recovery promoter |