TWI685565B - Nucleic acid extraction method and extraction card thereof - Google Patents

Nucleic acid extraction method and extraction card thereof Download PDF

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TWI685565B
TWI685565B TW107145107A TW107145107A TWI685565B TW I685565 B TWI685565 B TW I685565B TW 107145107 A TW107145107 A TW 107145107A TW 107145107 A TW107145107 A TW 107145107A TW I685565 B TWI685565 B TW I685565B
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chamber
waste liquid
extraction
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liquid chamber
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TW201932590A (en
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鍾崴宇
黃菘斌
劉興倫
高于凱
李怡臻
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台達電子工業股份有限公司
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    • B01D11/04—Solvent extraction of solutions which are liquid
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Abstract

An extraction cassette is provided. The extraction cassette includes an extraction module and a liquid receiving module. The liquid receiving module is communicated with the extraction module. The extraction module includes an extraction module body, an expansion room, a reaction room, a filter, a collection room, a first waste room and a second waste room. The expansion room is formed on the extraction module body. The reaction room is formed on the extraction module body. The reaction room includes a reaction room outlet. The filter is disposed in the reaction room and corresponds to the reaction room outlet. The collection room is formed on the extraction module body. The collection room is communicated to the reaction room outlet. The first waste room is formed on the extraction module body. The first waste room is communicated to the reaction room outlet. The second waste room is formed on the extraction module body. The second waste room is communicated to the reaction room outlet.

Description

核酸萃取方法及其萃取卡匣Nucleic acid extraction method and extraction cassette

本發明之實施例係有關於一種萃取卡匣，特別係有關於一種具有儲液模組之萃取卡匣。The embodiments of the present invention relate to an extraction cassette, and particularly to an extraction cassette with a liquid storage module.

習知之核酸萃取模組，主要僅提供核酸萃取的功能，並不具有可供容納廢液之廢液室設計。由於廢液可能回流而污染反應室內的濾膜，因此在習知概念中，廢液室在設置上不會鄰近收集室。由於廢液室與反應室需要設置於不同模組套件的情況下，萃取卡匣的尺寸變的十分龐大，不但佔據空間，也提升成本。The conventional nucleic acid extraction module mainly only provides the function of nucleic acid extraction, and does not have a waste liquid chamber design for containing waste liquid. Since the waste liquid may return and pollute the filter membrane in the reaction chamber, in the conventional concept, the waste liquid chamber will not be arranged adjacent to the collection chamber. Since the waste liquid chamber and the reaction chamber need to be installed in different module sets, the size of the extraction cassette becomes very large, which not only takes up space but also increases costs.

習知技術亦存在複合功能之(all-in-one)之核酸萃取模組，其係利用磁珠萃取方法來進行核酸提取，但是磁珠的萃取效率與純度不佳。The conventional technology also has an all-in-one nucleic acid extraction module, which uses a magnetic bead extraction method for nucleic acid extraction, but the extraction efficiency and purity of the magnetic beads are not good.

本發明之實施例係為了欲解決習知技術之問題而提供之一種萃取卡匣，包括一儲液模組以及一萃取模組。該萃取模組連通該儲液模組。該萃取模組包括一萃取模組本體、一擴充室、一反應室、一濾膜、一收集室、一第一廢液室以及一第二廢液室。擴充室形成於該萃取模組本體。反應室形成於該萃取模組本體，包括一反應室入口、一反應室出口以及一反應室連接缺口，其中，該擴充室連接該反應室連接缺口，該反應室連接缺口位於該反應室入口與該反應室出口之間。濾膜設於該反應室並對應該反應室出口。收集室形成於該萃取模組本體，並連通該反應室出口。第一廢液室形成於該萃取模組本體，該第一廢液室連通該反應室出口。第二廢液室形成於該萃取模組本體，該第二廢液室連通該反應室出口。The embodiment of the present invention provides an extraction cartridge for solving the problems of the conventional technology, which includes a liquid storage module and an extraction module. The extraction module communicates with the liquid storage module. The extraction module includes an extraction module body, an expansion chamber, a reaction chamber, a filter membrane, a collection chamber, a first waste liquid chamber and a second waste liquid chamber. The expansion chamber is formed in the extraction module body. The reaction chamber is formed in the extraction module body and includes a reaction chamber inlet, a reaction chamber outlet, and a reaction chamber connection gap, wherein the expansion chamber is connected to the reaction chamber connection gap, and the reaction chamber connection gap is located at the inlet of the reaction chamber and Between the outlets of the reaction chamber. The filter membrane is provided in the reaction chamber and corresponds to the outlet of the reaction chamber. The collection chamber is formed in the body of the extraction module and communicates with the outlet of the reaction chamber. A first waste liquid chamber is formed in the body of the extraction module, and the first waste liquid chamber communicates with the outlet of the reaction chamber. A second waste liquid chamber is formed in the body of the extraction module, and the second waste liquid chamber communicates with the outlet of the reaction chamber.

在一實施例中，該反應室包括一錐狀部，該濾膜設於該錐狀部，該反應室出口形成於該錐狀部之端部。In one embodiment, the reaction chamber includes a tapered portion, the filter membrane is disposed in the tapered portion, and the outlet of the reaction chamber is formed at an end of the tapered portion.

在一實施例中，該萃取模組更包括一第一通道以及一第二通道，該第一通道連通該反應室出口與該第一廢液室以及該第二廢液室，該第二通道連通該反應室出口與該收集室。In an embodiment, the extraction module further includes a first channel and a second channel, the first channel communicates the outlet of the reaction chamber with the first waste liquid chamber and the second waste liquid chamber, and the second channel Connect the outlet of the reaction chamber with the collection chamber.

在一實施例中，該第一通道以及該第二通道於該反應室出口相交，該第二通道內形成有一擋牆，該擋牆形成於該第二通道之端部並鄰接該反應室出口。In an embodiment, the first channel and the second channel intersect at the outlet of the reaction chamber, a blocking wall is formed in the second channel, the blocking wall is formed at the end of the second channel and is adjacent to the outlet of the reaction chamber .

在一實施例中，該萃取模組更包括一第三通道以及該第四通道，該第三通道連接該第一通道以及該第一廢液室，該第四通道連接該第一通道以及該第二廢液室。In an embodiment, the extraction module further includes a third channel and the fourth channel, the third channel is connected to the first channel and the first waste liquid chamber, and the fourth channel is connected to the first channel and the The second waste liquid chamber.

在一實施例中，該第一廢液室包括一第一廢液室連接孔，該第二廢液室包括一第二廢液室連接孔，該第三通道連接該第一通道以及該第一廢液室連接孔，該第四通道連接該第一通道以及該第二廢液室連接孔，該第二廢液室至少部分位於該第一廢液室連接孔與該第二廢液室連接孔之間。In an embodiment, the first waste liquid chamber includes a first waste liquid chamber connection hole, the second waste liquid chamber includes a second waste liquid chamber connection hole, and the third channel connects the first channel and the first A waste liquid chamber connecting hole, the fourth channel connects the first channel and the second waste liquid chamber connecting hole, the second waste liquid chamber is at least partially located in the first waste liquid chamber connecting hole and the second waste liquid chamber Between connecting holes.

在一實施例中，至少部分之該第四通道沿一第一方向延伸，該第一方向遠離該第一廢液室。In an embodiment, at least part of the fourth channel extends along a first direction, the first direction being away from the first waste liquid chamber.

在一實施例中，該第二廢液室包括一第二廢液室供壓孔，該第二廢液室至少部分位於該第一廢液室與該第二廢液室供壓孔之間。In one embodiment, the second waste liquid chamber includes a second waste liquid chamber pressure supply hole, the second waste liquid chamber is at least partially located between the first waste liquid chamber and the second waste liquid chamber pressure supply hole .

在一實施例中，至少部分之該第二通道沿該第一方向延伸。In an embodiment, at least part of the second channel extends along the first direction.

在一實施例中，該收集室包括一收集室供壓孔，該收集室至少部分位於該第一廢液室與該收集室供壓孔之間。In an embodiment, the collection chamber includes a collection chamber pressure supply hole, and the collection chamber is at least partially located between the first waste liquid chamber and the collection chamber pressure supply hole.

在一實施例中，該萃取模組更包括一吸附材，該吸附材設於該第一廢液室之中，該第一廢液室包括一第一廢液室供壓孔，該吸附材至少部分位於該第一廢液室供壓孔與該第一廢液室連接孔之間之該第一廢液室空間之中。In an embodiment, the extraction module further includes an adsorbent, the adsorbent is disposed in the first waste liquid chamber, the first waste liquid chamber includes a first waste liquid chamber supply hole, and the adsorbent It is at least partially located in the space of the first waste liquid chamber between the pressure supply hole of the first waste liquid chamber and the connecting hole of the first waste liquid chamber.

在一實施例中，該第一廢液室供壓孔、該第二廢液室供壓孔以及該收集室供壓孔位於同一平面。In an embodiment, the first waste liquid chamber pressure supply hole, the second waste liquid chamber pressure supply hole and the collection chamber pressure supply hole are located on the same plane.

在一實施例中，該擴充室更包括一擴充室進液口、一擴充室供壓孔以及一擴充室隔板，該擴充室隔板位於該擴充室進液口與該擴充室供壓孔之間，該擴充室隔板彎折偏向該擴充室供壓孔。In an embodiment, the expansion chamber further includes an expansion chamber inlet, an expansion chamber pressure hole, and an expansion chamber partition, the expansion chamber separator is located between the expansion chamber inlet and the expansion chamber pressure hole In between, the expansion chamber baffle is bent and biased toward the expansion chamber pressure supply hole.

在一實施例中，本發明另提供一種核酸萃取方法，包括下述步驟。首先，提供一萃取卡匣，該萃取卡匣包括一萃取模組以及一儲液模組，該儲液模組連通該萃取模組，該萃取模組包括一反應室、一濾膜、一收集室、一第一廢液室以及一第二廢液室。接著，將充分混合檢體以及酒精之一混合液從該儲液模組被注入該反應室，該濾膜從該混合液進行核酸抓取。再，將該混合液抽入該第一廢液室。接著，從該儲液模組使一第一清潔液經過該反應室以及該濾膜。再，將該第一清潔液抽入該第一廢液室。In an embodiment, the present invention further provides a nucleic acid extraction method, including the following steps. First, an extraction cassette is provided. The extraction cassette includes an extraction module and a liquid storage module. The liquid storage module communicates with the extraction module. The extraction module includes a reaction chamber, a filter membrane, and a collection. Chamber, a first waste liquid chamber and a second waste liquid chamber. Next, a mixed solution of one of sufficiently mixed specimen and alcohol is injected into the reaction chamber from the liquid storage module, and the filter membrane performs nucleic acid capture from the mixed solution. Then, the mixed liquid is drawn into the first waste liquid chamber. Next, a first cleaning liquid is passed through the reaction chamber and the filter membrane from the liquid storage module. Then, the first cleaning liquid is drawn into the first waste liquid chamber.

在一實施例中，該核酸萃取方法，其更包括下述步驟。首先，從該儲液模組使一第二清潔液經過該反應室以及該濾膜。接著，將該第二清潔液抽入該第二廢液室。再，從該儲液模組使一洗脫劑進入該經過該反應室，並靜置。接著，以該第二廢液室之該第二廢液室供壓孔與反應室之反應室入口提供正壓，並以該收集室之該收集室供壓孔提供負壓，以將含有核酸之該洗脫劑移入該收集室。In one embodiment, the nucleic acid extraction method further includes the following steps. First, a second cleaning liquid is passed through the reaction chamber and the filter membrane from the liquid storage module. Next, the second cleaning liquid is drawn into the second waste liquid chamber. Then, an eluent is allowed to enter the passing reaction chamber from the liquid storage module and is allowed to stand. Next, the positive pressure is provided by the second waste liquid chamber supply pressure hole of the second waste liquid chamber and the reaction chamber inlet of the reaction chamber, and the negative pressure is provided by the collection chamber supply pressure hole of the collection chamber to remove the nucleic acid The eluent moves into the collection chamber.

應用本發明實施例之萃取模組，由於反應室以及廢液室被整合於萃取模組之中，因此可縮小萃取卡匣的體積，並節省成本。特別是，透過流道、反應室、收集室、第一廢液室以及第二廢液室的配置，以及壓力的供給，可以控制廢液流入第一廢液室以及第二廢液室，並控制含有核酸之該洗脫劑流入收集室，並避免廢液污染反應室及收集室。With the extraction module according to the embodiment of the present invention, since the reaction chamber and the waste liquid chamber are integrated into the extraction module, the volume of the extraction cassette can be reduced and the cost can be saved. In particular, through the arrangement of the flow channel, the reaction chamber, the collection chamber, the first waste liquid chamber and the second waste liquid chamber, and the supply of pressure, the flow of waste liquid into the first waste liquid chamber and the second waste liquid chamber can be controlled, and Control the flow of the eluent containing nucleic acid into the collection chamber, and prevent the waste liquid from contaminating the reaction chamber and the collection chamber.

第1A圖係顯示本發明實施例之萃取卡匣C的組合圖。第1B圖係顯示本發明實施例之萃取卡匣C的***圖。請搭配參照第1A、1B圖，萃取卡匣C包括一萃取模組1、一儲液模組2、一取樣模組3、連通模組4等元件。萃取卡匣C適於置入於一分析儀之中。該分析儀包括一第一氣壓供應模組、一第二氣壓供應模組以及一檢測模組以及，該第一氣壓供應模組以及該第二氣壓供應模組對萃取卡匣C提供氣壓，以控制萃取卡匣C內的液體流動。該檢測模組對萃取卡匣C進行加熱、散熱，並對萃取卡匣C內之檢體進行檢測分析。FIG. 1A is a combination diagram of an extraction cartridge C according to an embodiment of the invention. FIG. 1B is an exploded view of the extraction cassette C according to an embodiment of the invention. Please refer to FIGS. 1A and 1B. The extraction cartridge C includes an extraction module 1, a storage module 2, a sampling module 3, a communication module 4, and other components. The extraction cassette C is suitable for being placed in an analyzer. The analyzer includes a first air pressure supply module, a second air pressure supply module, and a detection module, and the first air pressure supply module and the second air pressure supply module provide air pressure to the extraction cassette C, Control the flow of liquid in extraction cassette C. The detection module heats and dissipates the extraction cassette C, and detects and analyzes the specimens in the extraction cassette C.

請參照第1B圖，儲液模組2透過連通模組4連通萃取模組1。第2圖係顯示本發明實施例之萃取模組1的細部結構。參照第2圖，萃取模組1包括一萃取模組本體19、一擴充室18、一反應室11、一濾膜111、一收集室12、一第一廢液室13以及一第二廢液室14。擴充室18形成於萃取模組本體19。反應室11形成於萃取模組本體19，反應室11包括一反應室入口114、一反應室出口112以及一反應室連接缺口115，其中，擴充室18連接反應室連接缺口115，反應室連接缺口115位於反應室入口114與反應室出口112之間。濾膜111設於反應室11並對應反應室出口112。收集室12形成於萃取模組本體19，並連通反應室出口112。第一廢液室13形成於萃取模組本體19，第一廢液室13連通反應室出口112。第二廢液室14形成於萃取模組本體19，第二廢液室14連通反應室出口112。Please refer to FIG. 1B, the liquid storage module 2 communicates with the extraction module 1 through the communication module 4. FIG. 2 shows the detailed structure of the extraction module 1 according to an embodiment of the invention. Referring to FIG. 2, the extraction module 1 includes an extraction module body 19, an expansion chamber 18, a reaction chamber 11, a filter membrane 111, a collection chamber 12, a first waste liquid chamber 13 and a second waste liquid Room 14. The expansion chamber 18 is formed in the extraction module body 19. The reaction chamber 11 is formed in the extraction module body 19. The reaction chamber 11 includes a reaction chamber inlet 114, a reaction chamber outlet 112, and a reaction chamber connection gap 115, wherein the expansion chamber 18 is connected to the reaction chamber connection gap 115, and the reaction chamber connection gap 115 is located between the reaction chamber inlet 114 and the reaction chamber outlet 112. The filter 111 is disposed in the reaction chamber 11 and corresponds to the outlet 112 of the reaction chamber. The collection chamber 12 is formed in the extraction module body 19 and communicates with the outlet 112 of the reaction chamber. The first waste liquid chamber 13 is formed in the extraction module body 19, and the first waste liquid chamber 13 communicates with the outlet 112 of the reaction chamber. The second waste liquid chamber 14 is formed in the extraction module body 19, and the second waste liquid chamber 14 communicates with the reaction chamber outlet 112.

請參照第2圖，擴充室18更包括一擴充室進液口181、一擴充室供壓孔182以及一擴充室隔板183，擴充室隔板183位於擴充室進液口181與擴充室供壓孔182之間，擴充室隔板183彎折偏向擴充室供壓孔182。擴充室隔板183之設計防止經由擴充室進液口181進入擴充室18之液體污染擴充室供壓孔182。Referring to FIG. 2, the expansion chamber 18 further includes an expansion chamber inlet 181, an expansion chamber pressure hole 182, and an expansion chamber partition 183. The expansion chamber partition 183 is located at the expansion chamber inlet 181 and the expansion chamber supply Between the pressure holes 182, the expansion chamber partition 183 is bent and biased toward the expansion chamber supply pressure hole 182. The design of the expansion chamber partition 183 prevents the liquid entering the expansion chamber 18 through the expansion chamber inlet 181 from contaminating the expansion chamber supply hole 182.

在一實施例中，反應室11包括一錐狀部113，濾膜111設於錐狀部113，反應室出口112形成於錐狀部113之端部。在一實施例中，萃取模組1更包括一第一通道15以及一第二通道16，第一通道15連通反應室出口112與第一廢液室13以及第二廢液室14，第二通道16連通反應室出口112與收集室12。In an embodiment, the reaction chamber 11 includes a tapered portion 113, the filter membrane 111 is disposed in the tapered portion 113, and the reaction chamber outlet 112 is formed at an end of the tapered portion 113. In an embodiment, the extraction module 1 further includes a first channel 15 and a second channel 16, the first channel 15 communicates the reaction chamber outlet 112 with the first waste liquid chamber 13 and the second waste liquid chamber 14, the second The channel 16 communicates with the outlet 112 of the reaction chamber and the collection chamber 12.

在一實施例中，第一通道15以及該第二通道16於反應室出口112相交，第二通道16內形成有一擋牆161，擋牆161形成於第二通道16之端部並鄰接反應室出口112。在一實施例中，萃取模組1更包括一第三通道151以及第四通道152，第三通道151連接第一通道15以及第一廢液室13，第四通道152連接第一通道15以及第二廢液室14。在一實施例中，第一廢液室13包括一第一廢液室連接孔131，第二廢液室14包括一第二廢液室連接孔141，第三通道151連接第一通道15以及第一廢液室連接孔131，第四通道152連接第一通道15以及第二廢液室連接孔141，第二廢液室14至少部分位於第一廢液室連接孔131與第二廢液室連接孔141之間。在一實施例中，至少部分之第四通道152沿一第一方向Z延伸，第一方向Z遠離第一廢液室13，上述揭露並未限制本發明。In one embodiment, the first channel 15 and the second channel 16 intersect at the outlet 112 of the reaction chamber. A blocking wall 161 is formed in the second channel 16. The blocking wall 161 is formed at the end of the second channel 16 and is adjacent to the reaction chamber Exit 112. In an embodiment, the extraction module 1 further includes a third channel 151 and a fourth channel 152, the third channel 151 is connected to the first channel 15 and the first waste liquid chamber 13, and the fourth channel 152 is connected to the first channel 15 and Second waste liquid chamber 14. In an embodiment, the first waste liquid chamber 13 includes a first waste liquid chamber connection hole 131, the second waste liquid chamber 14 includes a second waste liquid chamber connection hole 141, and the third channel 151 is connected to the first channel 15 and The first waste liquid chamber connecting hole 131, the fourth channel 152 connects the first channel 15 and the second waste liquid chamber connecting hole 141, and the second waste liquid chamber 14 is at least partially located in the first waste liquid chamber connecting hole 131 and the second waste liquid Between the chamber connection holes 141. In an embodiment, at least part of the fourth channel 152 extends along a first direction Z, which is away from the first waste liquid chamber 13. The above disclosure does not limit the present invention.

在一實施例中，第二廢液室14包括一第二廢液室供壓孔142，第二廢液室14至少部分位於第一廢液室13與第二廢液室供壓孔142之間。在一實施例中，至少部分之第二通道16沿第一方向Z延伸，上述揭露並未限制本發明。在一實施例中，收集室12包括一收集室供壓孔121，收集室12至少部分位於第一廢液室13與收集室供壓孔121之間。In an embodiment, the second waste liquid chamber 14 includes a second waste liquid chamber pressure supply hole 142, and the second waste liquid chamber 14 is located at least partially between the first waste liquid chamber 13 and the second waste liquid chamber pressure supply hole 142 between. In one embodiment, at least part of the second channel 16 extends along the first direction Z. The above disclosure does not limit the present invention. In an embodiment, the collection chamber 12 includes a collection chamber pressure supply hole 121, and the collection chamber 12 is located at least partially between the first waste liquid chamber 13 and the collection chamber pressure supply hole 121.

在一實施例中，萃取模組1更包括一吸附材17，吸附材17設於第一廢液室13之中，第一廢液室13包括一第一廢液室供壓孔132，吸附材17至少部分位於第一廢液室供壓孔132與第一廢液室連接孔131之間之第一廢液室13空間之中。在一實施例中，吸附材17例如是一海綿。在一實施例中，第一廢液室供壓孔132、第二廢液室供壓孔142以及收集室供壓孔121位於同一平面。In an embodiment, the extraction module 1 further includes an adsorption material 17 disposed in the first waste liquid chamber 13. The first waste liquid chamber 13 includes a first waste liquid chamber supply pressure hole 132 for adsorption The material 17 is at least partially located in the space of the first waste liquid chamber 13 between the first waste liquid chamber supply hole 132 and the first waste liquid chamber connection hole 131. In one embodiment, the adsorption material 17 is, for example, a sponge. In one embodiment, the first waste liquid chamber pressure supply hole 132, the second waste liquid chamber pressure supply hole 142, and the collection chamber pressure supply hole 121 are located on the same plane.

搭配參照第2、3A～3J圖，其係顯示本發明實施例之儲液模組2的運作步驟。參照第3A圖，首先，充分混合檢體以及酒精之一混合液286從儲液模組2經過擴充室進液口181被注入擴充室18以及反應室11，濾膜111從混合液286進行核酸抓取。接著，參照第3B圖，透過第一廢液室供壓孔132提供負壓(＜-10Kpa)以將混合液286抽入第一廢液室13。再，參照第3C圖，從儲液模組2將一第一清潔液282分次經過擴充室進液口181、擴充室18、反應室11以及濾膜111，以調整濾膜111上之鹽類濃度與PH值。接著，參照第3D圖，透過第一廢液室供壓孔132提供負壓(＜-10Kpa)以將第一清潔液282抽入第一廢液室13。再，參照第3E圖，從儲液模組2將部分之一第二清潔液283經過擴充室進液口181、擴充室18、反應室11以及濾膜111，以調整濾膜111上之鹽類濃度與PH值。接著，參照第3F圖，透過第一廢液室供壓孔132提供負壓(＜-10Kpa)以將第二清潔液283抽入第一廢液室13。再，參照第3G圖，從儲液模組2將另一部分之第二清潔液283經過擴充室進液口181、擴充室18、反應室11以及濾膜111，以調整濾膜111上之鹽類濃度與PH值。接著，參照第3H圖，透過第二廢液室供壓孔142提供負壓(＜-10Kpa)以將第二清潔液283抽入第二廢液室14。再，參照第3I圖，從儲液模組2將一洗脫劑284經由反應室入口114注入經過反應室11，並靜置3分鐘，以使濾膜111上的核酸脫離。接著，參照第3J圖，透過施加一大正壓(45kpa)，以將含有核酸之洗脫劑284移入收集室12。搭配參照第2圖，在第3J圖之步驟中，第二廢液室供壓孔142提供小正壓，以防止含有核酸之洗脫劑284進入第一通道15。在一實施例中，該大正壓(45kpa)係透過儲液模組2而提供。With reference to Figures 2, 3A to 3J, it shows the operation steps of the liquid storage module 2 according to an embodiment of the present invention. Referring to FIG. 3A, first, a mixed solution 286 of one of the specimen and alcohol is thoroughly mixed, and is injected into the expansion chamber 18 and the reaction chamber 11 from the storage module 2 through the expansion chamber inlet 181, and the filter 111 performs nucleic acid from the mixed solution 286. Crawl. Next, referring to FIG. 3B, a negative pressure (<-10 Kpa) is supplied through the first waste liquid chamber pressure supply hole 132 to draw the mixed liquid 286 into the first waste liquid chamber 13. Then, referring to FIG. 3C, a first cleaning liquid 282 is passed through the expansion chamber inlet 181, the expansion chamber 18, the reaction chamber 11 and the filter 111 from the liquid storage module 2 in order to adjust the salt on the filter 111 Class concentration and PH value. Next, referring to FIG. 3D, a negative pressure (<-10 Kpa) is supplied through the first waste liquid chamber pressure supply hole 132 to draw the first cleaning liquid 282 into the first waste liquid chamber 13. 3E, from the liquid storage module 2, a part of the second cleaning liquid 283 passes through the expansion chamber inlet 181, the expansion chamber 18, the reaction chamber 11 and the filter 111 to adjust the salt on the filter 111 Class concentration and PH value. Next, referring to FIG. 3F, a negative pressure (<-10 Kpa) is supplied through the first waste liquid chamber pressure supply hole 132 to draw the second cleaning liquid 283 into the first waste liquid chamber 13. 3G, from the liquid storage module 2, another part of the second cleaning liquid 283 passes through the expansion chamber inlet 181, the expansion chamber 18, the reaction chamber 11 and the filter 111 to adjust the salt on the filter 111 Class concentration and PH value. Next, referring to FIG. 3H, a negative pressure (<-10 Kpa) is supplied through the second waste liquid chamber pressure supply hole 142 to draw the second cleaning liquid 283 into the second waste liquid chamber 14. Referring again to FIG. 3I, an eluent 284 is injected from the liquid storage module 2 through the reaction chamber inlet 114 through the reaction chamber 11, and is left to stand for 3 minutes to detach the nucleic acid on the filter 111. Next, referring to FIG. 3J, by applying a large positive pressure (45 kpa), the eluent 284 containing nucleic acid is moved into the collection chamber 12. With reference to FIG. 2, in the step of FIG. 3J, the second waste liquid chamber pressure supply hole 142 provides a small positive pressure to prevent the eluent 284 containing nucleic acid from entering the first channel 15. In one embodiment, the large positive pressure (45 kpa) is provided through the liquid storage module 2.

在上述第3D、3F圖之步驟中，第一清潔液282以及第二清潔液283的清潔次數可以為一次或一次以上，上述揭露並未限制本發明。在一實施例中，第一清潔液282用以中和pH值，第二清潔液283用於清洗蛋白質與有機物雜質。In the above steps of FIGS. 3D and 3F, the number of cleaning times of the first cleaning liquid 282 and the second cleaning liquid 283 may be one or more than one, and the above disclosure does not limit the present invention. In one embodiment, the first cleaning solution 282 is used to neutralize the pH value, and the second cleaning solution 283 is used to clean protein and organic impurities.

在上述實施例中，擴充室18之設計防止混合液286、第一清潔液282以及第二清潔液283的液面觸及反應室入口114，以防止洗脫劑284遭受污染。In the above embodiment, the design of the expansion chamber 18 prevents the liquid surfaces of the mixed liquid 286, the first cleaning liquid 282, and the second cleaning liquid 283 from touching the reaction chamber inlet 114, so as to prevent the eluent 284 from being contaminated.

請搭配參照第2圖，在上述實施例中，擋牆161之設計可防止混合液286、第一清潔液282以及第二清潔液283進入收集室12。在一實施例中，第二通道16具有一彎折部162，彎折部162之設計可使含有核酸之洗脫劑284充分的進入收集室12。在一實施例中，收集室12更包括一收集室斜面122以及一收集室出口123，收集室斜面122連接收集室出口123，收集室斜面122之設計可使含有核酸之洗脫劑284充分的經由收集室出口123流出(至取樣模組3，搭配參照第1B圖)。在一實施例中，彎折部162朝向收集室斜面122。Please refer to FIG. 2. In the above embodiment, the design of the retaining wall 161 prevents the mixed liquid 286, the first cleaning liquid 282 and the second cleaning liquid 283 from entering the collection chamber 12. In one embodiment, the second channel 16 has a bent portion 162. The bent portion 162 is designed to allow the eluent 284 containing nucleic acid to enter the collection chamber 12 sufficiently. In one embodiment, the collection chamber 12 further includes a collection chamber slope 122 and a collection chamber outlet 123. The collection chamber slope 122 is connected to the collection chamber outlet 123. The design of the collection chamber slope 122 enables the nucleic acid-containing eluent 284 to be sufficiently It flows out through the outlet 123 of the collection chamber (to the sampling module 3, see Figure 1B for coordination). In one embodiment, the bent portion 162 faces the inclined surface 122 of the collection chamber.

搭配參照第2圖，在第3H圖之步驟中，最後的部分之第二清潔液283被抽入第二廢液室14。第二廢液室14中的廢液(該第二清潔液283)為最乾淨的廢液。在第3J圖之步驟中，第二廢液室供壓孔142提供小正壓，以防止含有核酸之洗脫劑284進入第一通道1。由於第二廢液室14中的廢液(該第二清潔液283)為最乾淨的廢液，因此在第二廢液室供壓孔142提供小正壓的情況下，第二廢液室14中的氣體對含有核酸之洗脫劑284的污染將降至最低。With reference to FIG. 2, in the step of FIG. 3H, the last part of the second cleaning liquid 283 is drawn into the second waste liquid chamber 14. The waste liquid in the second waste liquid chamber 14 (the second cleaning liquid 283) is the cleanest waste liquid. In the step of FIG. 3J, the second waste liquid chamber supply hole 142 provides a small positive pressure to prevent the eluent 284 containing nucleic acid from entering the first channel 1. Since the waste liquid in the second waste liquid chamber 14 (the second cleaning liquid 283) is the cleanest waste liquid, when the second waste liquid chamber supply hole 142 provides a small positive pressure, the second waste liquid chamber The gas in 14 will minimize the contamination of eluent 284 containing nucleic acids.

搭配參照第2圖，在第3F圖的步驟中，第一廢液室13內的廢液將接觸到吸附材17，吸附材17可消除廢液的氣泡，避免第一廢液室13內的廢液從該第一廢液室供壓孔132溢出。在一實施例中，濾膜111可以為矽濾膜或其他材質之濾膜。在一實施例中，萃取模組1為一體成型。With reference to FIG. 2, in the step of FIG. 3F, the waste liquid in the first waste liquid chamber 13 will contact the adsorbent 17. The adsorbent 17 can eliminate the bubbles of the waste liquid and avoid the waste in the first waste liquid chamber 13. The waste liquid overflows from the pressure supply hole 132 of the first waste liquid chamber. In one embodiment, the filter 111 may be a silicon filter or a filter made of other materials. In one embodiment, the extraction module 1 is integrally formed.

參照第4圖，在一實施例中，本發明另提供一種核酸萃取方法，包括下述步驟。首先，提供一萃取卡匣，該萃取卡匣包括一萃取模組以及一儲液模組，該儲液模組連通該萃取模組，該萃取模組包括一反應室、一濾膜、一收集室、一第一廢液室以及一第二廢液室(S11)。接著，將充分混合檢體以及酒精之一混合液從該儲液模組被注入該反應室，該濾膜從該混合液進行核酸抓取(S12)。再者，將該混合液抽入該第一廢液室(S13)。接著，從該儲液模組使一第一清潔液經過該反應室以及該濾膜(S14)。再者，將該第一清潔液抽入該第一廢液室(S15)。接著，從該儲液模組使一第二清潔液經過該反應室以及該濾膜(S16)。接著，將該第二清潔液抽入該第二廢液室(S17)。再者，從該儲液模組使一洗脫劑進入該經過該反應室，並靜置(S18)。接著，以該第二廢液室之該第二廢液室供壓孔提供正壓，並以該收集室之該收集室供壓孔提供負壓，以將含有核酸之該洗脫劑移入該收集室(S19)。Referring to FIG. 4, in an embodiment, the present invention further provides a nucleic acid extraction method, including the following steps. First, an extraction cassette is provided. The extraction cassette includes an extraction module and a liquid storage module. The liquid storage module communicates with the extraction module. The extraction module includes a reaction chamber, a filter membrane, and a collection. Chamber, a first waste liquid chamber and a second waste liquid chamber (S11). Next, a mixed solution in which one of the sample and alcohol is sufficiently mixed is injected into the reaction chamber from the storage module, and the filter membrane performs nucleic acid capture from the mixed solution (S12). Furthermore, the mixed liquid is drawn into the first waste liquid chamber (S13). Next, a first cleaning liquid is passed through the reaction chamber and the filter membrane from the liquid storage module (S14). Furthermore, the first cleaning liquid is drawn into the first waste liquid chamber (S15). Next, a second cleaning liquid is passed through the reaction chamber and the filter membrane from the liquid storage module (S16). Next, the second cleaning liquid is drawn into the second waste liquid chamber (S17). Furthermore, an eluent is allowed to enter the passing reaction chamber from the liquid storage module and stand still (S18). Next, the second waste liquid chamber pressure supply hole of the second waste liquid chamber is used to provide positive pressure, and the collection chamber pressure supply hole of the collection chamber is used to provide negative pressure to move the eluent containing nucleic acid into the Collection room (S19).

應用本發明實施例之萃取模組，由於反應室以及廢液室被整合於萃取模組之中，因此可縮小萃取卡匣的體積，並節省成本。特別是，透過流道、反應室、收集室、第一廢液室以及第二廢液室的配置，以及壓力的供給，可以控制廢液流入第一廢液室以及第二廢液室，並控制含有核酸之該洗脫劑流入收集室，並避免廢液污染反應室及收集室。With the extraction module according to the embodiment of the present invention, since the reaction chamber and the waste liquid chamber are integrated into the extraction module, the volume of the extraction cassette can be reduced and the cost can be saved. In particular, through the arrangement of the flow channel, the reaction chamber, the collection chamber, the first waste liquid chamber and the second waste liquid chamber, and the supply of pressure, the flow of waste liquid into the first waste liquid chamber and the second waste liquid chamber can be controlled, and Control the flow of the eluent containing nucleic acid into the collection chamber, and prevent the waste liquid from contaminating the reaction chamber and the collection chamber.

雖然本發明已以具體之較佳實施例揭露如上，然其並非用以限定本發明，任何熟習此項技術者，在不脫離本發明之精神和範圍內，仍可作些許的更動與潤飾，因此本發明之保護範圍當視後附之申請專利範圍所界定者為準。Although the present invention has been disclosed as above with specific preferred embodiments, it is not intended to limit the present invention. Anyone who is familiar with this technology can still make some changes and modifications without departing from the spirit and scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the scope defined in the appended patent application.

C‧‧‧萃取卡匣1‧‧‧萃取模組11‧‧‧反應室111‧‧‧濾膜112‧‧‧反應室出口113‧‧‧錐狀部114‧‧‧反應室入口115‧‧‧反應室連接缺口12‧‧‧收集室121‧‧‧收集室供壓孔122‧‧‧收集室斜面123‧‧‧收集室出口13‧‧‧第一廢液室131‧‧‧第一廢液室連接孔132‧‧‧第一廢液室供壓孔14‧‧‧第二廢液室141‧‧‧第二廢液室連接孔142‧‧‧第二廢液室供壓孔15‧‧‧第一通道151‧‧‧第三通道152‧‧‧第四通道16‧‧‧第二通道C‧‧‧extraction cassette 1‧‧‧extraction module 11‧‧‧reaction chamber 111‧‧‧filter membrane 112‧‧‧reaction chamber outlet 113‧‧‧conical part 114‧‧‧reaction chamber inlet 115‧‧ ‧Reaction chamber connection gap 12‧‧‧Collection chamber 121‧‧‧Collection chamber pressure hole 122‧‧‧Collection chamber slope 123‧‧‧Collection chamber outlet 13‧‧‧First waste liquid chamber 131‧‧‧First waste Liquid chamber connection hole 132‧‧‧ First waste liquid chamber pressure hole 14‧‧‧ Second waste liquid chamber 141‧‧‧ Second waste liquid chamber connection hole 142‧‧‧Second waste liquid chamber pressure hole 15‧ ‧‧ First channel 151‧‧‧ Third channel 152‧‧‧ Fourth channel 16‧‧‧ Second channel

161‧‧‧擋牆 161‧‧‧ Retaining wall

162‧‧‧彎折部 162‧‧‧Bending Department

17‧‧‧吸附材 17‧‧‧Adsorption material

18‧‧‧擴充室 18‧‧‧Expansion room

181‧‧‧擴充室進液口 181‧‧‧Expansion chamber inlet

182‧‧‧擴充室供壓孔 182‧‧‧Expansion chamber pressure hole

183‧‧‧擴充室隔板 183‧‧‧Expansion room partition

19‧‧‧萃取模組本體 19‧‧‧ Extraction module body

2‧‧‧儲液模組 2‧‧‧Liquid storage module

282‧‧‧第一清潔液 282‧‧‧First cleaning fluid

283‧‧‧第二清潔液 283‧‧‧Second cleaning fluid

284‧‧‧洗脫劑 284‧‧‧Eluent

286‧‧‧混合液 286‧‧‧mixed liquid

3‧‧‧取樣模組 3‧‧‧Sampling module

4‧‧‧連通模組 4‧‧‧Connect module

S11、S12、S13、S14、S15、S16、S17、S18、S19‧‧‧步驟 S11, S12, S13, S14, S15, S16, S17, S18, S19

Z‧‧‧第一方向 Z‧‧‧First direction

第1A圖係顯示本發明實施例之萃取卡匣的組合圖。 第1B圖係顯示本發明實施例之萃取卡匣的***圖。 第2圖係顯示本發明實施例之萃取模組的細部結構。 第3A、3B、3C、3D、3E、3F、3G、3H、3I、3J圖係顯示本發明實施例之儲液模組的運作步驟。 第4圖係顯示本發明實施例之核酸萃取方法之流程圖。FIG. 1A is an assembly diagram of an extraction cassette according to an embodiment of the invention. FIG. 1B is an exploded view of an extraction cassette according to an embodiment of the invention. Figure 2 shows the detailed structure of the extraction module according to an embodiment of the present invention. Figures 3A, 3B, 3C, 3D, 3E, 3F, 3G, 3H, 3I, and 3J show the operation steps of the liquid storage module according to an embodiment of the present invention. FIG. 4 is a flowchart showing a nucleic acid extraction method according to an embodiment of the invention.

1‧‧‧萃取模組 1‧‧‧Extraction module

11‧‧‧反應室 11‧‧‧ Reaction room

111‧‧‧濾膜 111‧‧‧ filter membrane

112‧‧‧反應室出口 112‧‧‧Exit of the reaction chamber

113‧‧‧錐狀部 113‧‧‧Cone

114‧‧‧反應室入口 114‧‧‧ Entrance of the reaction chamber

115‧‧‧反應室連接缺口 115‧‧‧ Reaction chamber connection gap

12‧‧‧收集室 12‧‧‧ Collection room

121‧‧‧收集室供壓孔 121‧‧‧Compression chamber supply hole

122‧‧‧收集室斜面 122‧‧‧Slope of the collection room

123‧‧‧收集室出口 123‧‧‧ Collection room exit

13‧‧‧第一廢液室 13‧‧‧The first waste liquid room

131‧‧‧第一廢液室連接孔 131‧‧‧ Connection hole of the first waste liquid chamber

132‧‧‧第一廢液室供壓孔 132‧‧‧ Pressure hole for the first waste liquid chamber

14‧‧‧第二廢液室 14‧‧‧Second waste liquid chamber

141‧‧‧第二廢液室連接孔 141‧‧‧ Connection hole of the second waste liquid chamber

142‧‧‧第二廢液室供壓孔 142‧‧‧Pressure hole of the second waste liquid chamber

15‧‧‧第一通道 15‧‧‧ First channel

151‧‧‧第三通道 151‧‧‧ Third Channel

152‧‧‧第四通道 152‧‧‧The fourth channel

16‧‧‧第二通道 16‧‧‧Second channel

161‧‧‧擋牆 161‧‧‧ Retaining wall

162‧‧‧彎折部 162‧‧‧Bending Department

17‧‧‧吸附材 17‧‧‧Adsorption material

18‧‧‧擴充室 18‧‧‧Expansion room

181‧‧‧擴充室進液口 181‧‧‧Expansion chamber inlet

182‧‧‧擴充室供壓孔 182‧‧‧Expansion chamber pressure hole

183‧‧‧擴充室隔板 183‧‧‧Expansion room partition

19‧‧‧萃取模組本體 19‧‧‧ Extraction module body

Z‧‧‧第一方向 Z‧‧‧First direction

Claims (15)

一種萃取卡匣，包括：一儲液模組；以及一萃取模組，連通該儲液模組，包括：一萃取模組本體；一擴充室，形成於該萃取模組本體；一反應室，形成於該萃取模組本體，包括一反應室入口、一反應室出口以及一反應室連接缺口，其中，該擴充室連接該反應室連接缺口，該反應室連接缺口位於該反應室入口與該反應室出口之間；一濾膜，設於該反應室並對應該反應室出口；一收集室，形成於該萃取模組本體，並連通該反應室出口；一第一廢液室，形成於該萃取模組本體，該第一廢液室連通該反應室出口；以及一第二廢液室，形成於該萃取模組本體，該第二廢液室連通該反應室出口，其中，該擴充室更包括一擴充室進液口以及一擴充室供壓孔。 An extraction cassette includes: a liquid storage module; and an extraction module connected to the liquid storage module, including: an extraction module body; an expansion chamber formed in the extraction module body; a reaction chamber, The extraction module body includes a reaction chamber inlet, a reaction chamber outlet, and a reaction chamber connection gap, wherein the expansion chamber is connected to the reaction chamber connection gap, and the reaction chamber connection gap is located at the reaction chamber entrance and the reaction Between the outlets of the chamber; a filter membrane, which is provided in the reaction chamber and corresponds to the outlet of the reaction chamber; a collection chamber, formed in the body of the extraction module, and connected to the outlet of the reaction chamber; a first waste liquid chamber, formed in An extraction module body, the first waste liquid chamber communicates with the outlet of the reaction chamber; and a second waste liquid chamber formed in the extraction module body, the second waste fluid chamber communicates with the outlet of the reaction chamber, wherein the expansion chamber It also includes an expansion chamber inlet and an expansion chamber pressure hole. 如申請專利範圍第1項所述之萃取卡匣，其中該反應室包括一錐狀部，該濾膜設於該錐狀部，該反應室出口形成於該錐狀部之端部。 The extraction cartridge as described in item 1 of the patent application scope, wherein the reaction chamber includes a cone-shaped portion, the filter membrane is disposed in the cone-shaped portion, and the reaction chamber outlet is formed at an end of the cone-shaped portion. 如申請專利範圍第1項所述之萃取卡匣，其中該萃取模組更包括一第一通道以及一第二通道，該第一通道連通該反應室 出口與該第一廢液室以及該第二廢液室，該第二通道連通該反應室出口與該收集室。 The extraction cartridge as described in item 1 of the patent application scope, wherein the extraction module further includes a first channel and a second channel, the first channel communicates with the reaction chamber The outlet is connected to the first waste liquid chamber and the second waste liquid chamber, and the second channel communicates the reaction chamber outlet and the collection chamber. 如申請專利範圍第3項所述之萃取卡匣，其中該第一通道以及該第二通道於該反應室出口相交，該第二通道內形成有一擋牆，該擋牆形成於該第二通道之端部並鄰接該反應室出口。 The extraction cartridge according to item 3 of the patent application scope, wherein the first channel and the second channel intersect at the outlet of the reaction chamber, a blocking wall is formed in the second channel, and the blocking wall is formed in the second channel And the end of the reaction chamber is adjacent to the outlet. 如申請專利範圍第3項所述之萃取卡匣，其中該萃取模組更包括一第三通道以及該第四通道，該第三通道連接該第一通道以及該第一廢液室，該第四通道連接該第一通道以及該第二廢液室。 The extraction cartridge as described in item 3 of the patent application scope, wherein the extraction module further includes a third channel and the fourth channel, the third channel is connected to the first channel and the first waste liquid chamber, the first Four channels connect the first channel and the second waste liquid chamber. 如申請專利範圍第5項所述之萃取卡匣，其中該第一廢液室包括一第一廢液室連接孔，該第二廢液室包括一第二廢液室連接孔，該第三通道連接該第一通道以及該第一廢液室連接孔，該第四通道連接該第一通道以及該第二廢液室連接孔，該第二廢液室至少部分位於該第一廢液室連接孔與該第二廢液室連接孔之間。 The extraction cartridge according to item 5 of the patent application scope, wherein the first waste liquid chamber includes a first waste liquid chamber connection hole, the second waste liquid chamber includes a second waste liquid chamber connection hole, and the third The channel connects the first channel and the first waste liquid chamber connection hole, the fourth channel connects the first channel and the second waste liquid chamber connection hole, and the second waste liquid chamber is at least partially located in the first waste liquid chamber Between the connecting hole and the connecting hole of the second waste liquid chamber. 如申請專利範圍第6項所述之萃取卡匣，其中至少部分之該第四通道沿一第一方向延伸，該第一方向遠離該第一廢液室。 The extraction cartridge according to item 6 of the patent application scope, wherein at least part of the fourth channel extends along a first direction, and the first direction is away from the first waste liquid chamber. 如申請專利範圍第6項所述之萃取卡匣，其中該第二廢液室包括一第二廢液室供壓孔，該第二廢液室至少部分位於該第一廢液室與該第二廢液室供壓孔之間。 The extraction cartridge as described in item 6 of the patent application scope, wherein the second waste liquid chamber includes a second waste liquid chamber pressure supply hole, and the second waste liquid chamber is at least partially located in the first waste liquid chamber and the first Between the supply holes of the two waste liquid chambers. 如申請專利範圍第8項所述之萃取卡匣，其中至少部分之該第二通道沿該第一方向延伸。 The extraction cartridge as described in item 8 of the patent application scope, wherein at least part of the second channel extends along the first direction. 如申請專利範圍第8項所述之萃取卡匣，其中該收集室包括一收集室供壓孔，該收集室至少部分位於該第一廢液室與該收集室供壓孔之間。 The extraction cartridge according to item 8 of the patent application scope, wherein the collection chamber includes a collection chamber pressure supply hole, and the collection chamber is at least partially located between the first waste liquid chamber and the collection chamber pressure supply hole. 如申請專利範圍第10項所述之萃取卡匣，其中該萃取模組更包括一吸附材，該吸附材設於該第一廢液室之中，該第一廢液室包括一第一廢液室供壓孔，該吸附材至少部分位於該第一廢液室供壓孔與該第一廢液室連接孔之間之該第一廢液室空間之中。 The extraction cartridge as described in item 10 of the patent application scope, wherein the extraction module further includes an adsorbent material, the adsorbent material is disposed in the first waste liquid chamber, and the first waste liquid chamber includes a first waste In the liquid chamber pressure supply hole, the adsorbent is at least partially located in the space of the first waste liquid chamber between the first waste liquid chamber pressure supply hole and the first waste liquid chamber connection hole. 如申請專利範圍第11項所述之萃取卡匣，其中該第一廢液室供壓孔、該第二廢液室供壓孔以及該收集室供壓孔位於同一平面。 The extraction cartridge as described in item 11 of the patent application scope, wherein the first waste liquid chamber pressure supply hole, the second waste liquid chamber pressure supply hole and the collection chamber pressure supply hole are located on the same plane. 如申請專利範圍第1項所述之萃取卡匣，其中該擴充室更包括一擴充室隔板，該擴充室隔板位於該擴充室進液口與該擴充室供壓孔之間，該擴充室隔板彎折偏向該擴充室供壓孔。 The extraction cartridge as described in item 1 of the patent application scope, wherein the expansion chamber further includes an expansion chamber partition, the expansion chamber partition is located between the expansion chamber inlet and the expansion chamber supply hole, the expansion The chamber partition is bent and biased toward the pressure supply hole of the expansion chamber. 一種核酸萃取方法，包括：提供一萃取卡匣，該萃取卡匣包括一萃取模組以及一儲液模組，該儲液模組連通該萃取模組，該萃取模組包括一反應室、一濾膜、一收集室、一第一廢液室以及一第二廢液室；將充分混合檢體以及酒精之一混合液從該儲液模組被注入該反應室，該濾膜從該混合液進行核酸抓取；將該混合液抽入該第一廢液室；從該儲液模組使一第一清潔液經過該反應室以及該濾膜；以及將該第一清潔液抽入該第一廢液室。 A nucleic acid extraction method includes: providing an extraction cassette, the extraction cassette includes an extraction module and a liquid storage module, the liquid storage module communicates with the extraction module, the extraction module includes a reaction chamber, a Filter membrane, a collection chamber, a first waste fluid chamber, and a second waste fluid chamber; a mixture of fully mixed specimen and alcohol is injected into the reaction chamber from the storage module, and the filter membrane is mixed from the Nucleic acid grabbing; pumping the mixed liquid into the first waste liquid chamber; passing a first cleaning liquid from the liquid storage module through the reaction chamber and the filter membrane; and pumping the first cleaning liquid into the The first waste chamber. 如申請專利範圍第14項所述之核酸萃取方法，其更包括： 從該儲液模組使一第二清潔液經過該反應室以及該濾膜；將該第二清潔液抽入該第二廢液室；從該儲液模組使一洗脫劑進入該經過該反應室，並靜置；以及以該第二廢液室之一第二廢液室供壓孔提供正壓，並以該收集室之一收集室供壓孔提供負壓，以將含有核酸之該洗脫劑移入該收集室。 The nucleic acid extraction method as described in item 14 of the patent application scope further includes: From the liquid storage module, a second cleaning liquid is passed through the reaction chamber and the filter membrane; the second cleaning liquid is drawn into the second waste liquid chamber; from the liquid storage module, an eluent is entered into the passage The reaction chamber, and let it stand; and the positive pressure is provided by the second waste liquid chamber pressure supply hole of one of the second waste liquid chambers, and the negative pressure is provided by the collection chamber pressure supply hole of one of the collection chambers The eluent moves into the collection chamber.
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