TWI667343B - Specimen container - Google Patents

Specimen container Download PDF

Info

Publication number
TWI667343B
TWI667343B TW107117669A TW107117669A TWI667343B TW I667343 B TWI667343 B TW I667343B TW 107117669 A TW107117669 A TW 107117669A TW 107117669 A TW107117669 A TW 107117669A TW I667343 B TWI667343 B TW I667343B
Authority
TW
Taiwan
Prior art keywords
section
cross
liquid
hole
sample container
Prior art date
Application number
TW107117669A
Other languages
Chinese (zh)
Other versions
TW201907000A (en
Inventor
生藤邦夫
Original Assignee
日商斯庫林集團股份有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 日商斯庫林集團股份有限公司 filed Critical 日商斯庫林集團股份有限公司
Publication of TW201907000A publication Critical patent/TW201907000A/en
Application granted granted Critical
Publication of TWI667343B publication Critical patent/TWI667343B/en

Links

Landscapes

  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

本發明可於供培養細胞等之樣品容器中，藉由目視簡單地判定容器中承載之液體之量。本發明之樣品容器具有能夠於上部開口之內部空間SP儲存液體之孔。於水平姿勢之樣品容器中，將投影於水平面之內部空間之開口設為開口面111，將自開口鉛直向下特定之第1深度之位置處之內部空間SP之水平剖面設為第1剖面，將較第1深度更深之第2深度位置處之內部空間之水平剖面設為第2剖面時，開口面、第1剖面及第2剖面之外周形狀彼此不相似，且於俯視下，第2剖面包含於第1剖面內，第1剖面包含於上述開口面內。The present invention can easily determine the amount of liquid carried in the container by visual observation in a sample container for culturing cells or the like. The sample container of the present invention has a hole capable of storing a liquid in the inner space SP of the upper opening. In the sample container in the horizontal posture, the opening projected in the internal space of the horizontal plane is the opening surface 111, and the horizontal cross section of the internal space SP at the position of the first depth specified by the vertical downward direction is referred to as the first cross section. When the horizontal cross section of the internal space at the second depth position deeper than the first depth is the second cross section, the outer peripheral shapes of the opening surface, the first cross section, and the second cross section are not similar to each other, and the second cross section in plan view The first cross section is included in the first cross section, and the first cross section is included in the opening surface.

Description

樣品容器Sample container

本發明係關於一種保持包含液體之樣品之樣品容器，尤其關於一種適合於在液體中培養細胞等之樣品容器。The present invention relates to a sample container for holding a sample containing a liquid, and more particularly to a sample container suitable for culturing cells or the like in a liquid.

於醫學或生化學之技術領域中，為了進行各種實驗，例如利用顯微鏡觀察細胞等，而於注入容器中之液體中進行細胞或微生物、組織等(以下，稱為「細胞等」)之培養。例如，被稱為微量盤、孔板、微量滴定盤等之樣品容器係將被稱為孔之複數個凹窪矩陣配置而成者，該孔係於板狀基材之上表面具有開口且於內部空間可承載液體。In the field of medicine or biochemistry, in order to carry out various experiments, for example, observation of cells by a microscope, culture of cells, microorganisms, tissues, or the like (hereinafter referred to as "cells") is carried out in a liquid injected into a container. For example, a sample container called a microplate, an orifice plate, a microtiter plate, or the like is formed by a plurality of concave matrix arrays called holes, which have openings on the upper surface of the plate substrate and The internal space can carry liquid.

設置於此種容器之孔之一般形態係將具有圓形或矩形等相對簡單之形狀之水平剖面的筒形側壁面與平坦之底面組合而成。另一方面，出於特殊目的，有於孔之深度方向對剖面形狀施加變化之例。The general form of the hole provided in such a container is a combination of a cylindrical side wall surface having a horizontal cross section of a relatively simple shape such as a circular shape or a rectangular shape, and a flat bottom surface. On the other hand, for special purposes, there is an example in which the depth direction of the hole is changed to the shape of the cross section.

例如於專利文獻1(日本專利特開2016-063779號公報)中記載之技術中，於孔之底面形成複數個朝上之突起部。又，孔之側壁面成為隨著靠近底面而水平截面面積變小之曲面。此種構造之目的在於使細胞高效率地於突起部之上立體成長，且使其易於觀察。又，於專利文獻2(日本專利特開第2013/030940號說明書)中記載之技術中，於孔之底面，設置有用於培養細胞之突起部、及表示用於在觀察時能夠識別各個孔之記號等的突起部。For example, in the technique described in the patent document 1 (JP-A-2016-063779), a plurality of upwardly projecting projections are formed on the bottom surface of the hole. Further, the side wall surface of the hole becomes a curved surface whose horizontal cross-sectional area becomes smaller as it approaches the bottom surface. The purpose of such a configuration is to allow cells to grow sterically over the protrusions and make them easy to observe. Further, in the technique described in Patent Document 2 (Japanese Patent Laid-Open Publication No. 2013/030940), a projection for culturing a cell and a hole for recognizing each hole during observation are provided on the bottom surface of the hole. A protrusion such as a mark.

[發明所欲解決之問題][The problem that the invention wants to solve]

上述先前技術之樣品容器主要對便於觀察進行了研究。另一方面，於注入於樣品容器之液體中培養細胞等之情形時，必須應對與上述不同之問題。例如，培養液中之細胞之培養需在維持為恆溫、恆濕狀態之培養環境下進行，但於此種培養環境中培養液容易蒸發。因此，尋求可判別培養中之孔中的培養液之量是否合適之技術。尤其於多個孔矩陣配置而成之孔板等樣品容器中，難以自側方觀察各孔之液面。因此，所期望的是設法可藉由自上方之目視簡易地確認液量。然而，至今為止還未提出此種技術。The sample containers of the prior art described above were mainly studied for ease of observation. On the other hand, when cells or the like are cultured in a liquid injected into a sample container, it is necessary to cope with a problem different from the above. For example, the culture of the cells in the culture solution is carried out in a culture environment maintained in a constant temperature and a constant humidity state, but the culture solution is likely to evaporate in such a culture environment. Therefore, a technique for discriminating whether or not the amount of the culture solution in the well in the culture is appropriate is sought. In particular, in a sample container such as an orifice plate in which a plurality of pore matrices are arranged, it is difficult to observe the liquid surface of each well from the side. Therefore, it is desirable to try to easily confirm the amount of liquid by visual observation from above. However, such a technique has not been proposed so far.

若孔內之液量不足，則根據需要進行培養液之補充。又，為了去除因細胞等之活動生成之代謝物，亦有定期進行培養液之更換之情形。此時之培養液之量或狀態於各孔有差異，故操作者需要對各孔之培養液之狀態個別判斷並根據需要進行培養液之補充或更換。對孔之培養液之注入例如使用前端較細之移液管等器具進行。然而，並不容易利用手動作業將此種器具之前端相對於孔迅速且準確地定位。因此，於無法對孔注入準確量之液體，導致於作業上耗費時間等作業性方面存在問題。又，亦有可能器具之前端接觸到培養之細胞等而使細胞等損傷。 [解決問題之技術手段]If the amount of liquid in the well is insufficient, the culture solution is replenished as needed. Further, in order to remove metabolites generated by activities such as cells, it is also possible to periodically replace the culture solution. At this time, the amount or state of the culture solution differs between the respective wells, so the operator needs to individually judge the state of the culture solution of each well and replenish or replace the culture solution as needed. The injection of the culture solution of the well is carried out, for example, using an instrument such as a pipette having a narrow tip. However, it is not easy to use a manual operation to quickly and accurately position the front end of such an appliance relative to the aperture. Therefore, it is impossible to inject an accurate amount of liquid into the hole, which causes problems in workability such as time-consuming work. Further, there is a possibility that the front end of the device comes into contact with the cultured cells or the like to damage the cells or the like. [Technical means to solve the problem]

本發明係鑒於上述問題而完成者，其第1目的在於提供一種能夠於供培養細胞等之樣品容器中，藉由目視簡易地判斷承載於容器之液體量之技術。進而，其第2目的在於提供一種容易對樣品容器進行液體之注入作業之方法。The present invention has been made in view of the above problems, and a first object thereof is to provide a technique capable of easily determining the amount of liquid carried in a container by visual observation in a sample container for culturing cells or the like. Further, a second object thereof is to provide a method of easily performing a liquid injection operation on a sample container.

本發明之一態樣係一種樣品容器，其具有能夠於上部開口之內部空間儲存液體之孔，為了達成上述第1目的，於水平姿勢之上述樣品容器中，將投影於水平面之上述內部空間之開口設為開口面，將自上述開口鉛直向下特定之第1深度之位置處之上述內部空間之水平剖面設為第1剖面，將較上述第1深度更深之第2深度位置處之上述內部空間之水平剖面設為第2剖面時，上述開口面、上述第1剖面及上述第2剖面之外周形狀彼此不相似，且於俯視下，上述第2剖面包含於第1剖面內，上述第1剖面包含於上述開口面內。An aspect of the present invention is a sample container having a hole capable of storing a liquid in an inner space of an upper opening, and in order to achieve the above first object, the sample container in a horizontal posture is projected onto the inner space of the horizontal plane. The opening is an opening surface, and a horizontal cross section of the internal space at a position at which the opening is vertically downward is defined as a first cross section, and the inner portion at a second depth position deeper than the first depth When the horizontal cross section of the space is the second cross section, the outer peripheral shapes of the opening surface, the first cross section, and the second cross section are not similar to each other, and the second cross section is included in the first cross section in plan view, and the first The section is included in the open face.

更具體而言，可構成為，設置有自上述孔之側壁面朝向上述內部空間之中央部側突出之突出部，上述突出部包含出現於上述第1剖面而未出現於上述第2剖面之部位、及出現於上述第1剖面及上述第2剖面兩者之部位。或者，可構成為，上述孔之側壁面之一部分成為朝自上述內部空間向外側之方向突出之突出部，上述突出部包含出現於上述第1剖面而未出現於上述第2剖面之部位、及出現於上述第1剖面及上述第2剖面兩者之部位。More specifically, the protruding portion may protrude from a side wall surface of the hole toward a central portion side of the internal space, and the protruding portion may include a portion that appears in the first cross section and does not appear in the second cross section. And appearing in both the first cross section and the second cross section. Alternatively, one of the side wall surfaces of the hole may be a protruding portion that protrudes outward from the inner space, and the protruding portion includes a portion that appears in the first cross section and does not appear in the second cross section, and Appears in both the first cross section and the second cross section.

又，本發明之另一態樣係一種樣品容器，其具有能夠於上部開口之內部空間儲存液體之孔，為了達成上述第1目的，設置有自上述孔之底部沿上述孔之內壁面朝上突出之突出部，於水平姿勢之上述樣品容器中，將自上述內部空間之開口鉛直向下特定之第1深度之位置處之上述內部空間之水平剖面設為第1剖面，將較上述第1深度更深之第2深度位置處之上述內部空間之水平剖面設為第2剖面時，於俯視下，上述第2剖面包含於上述第1剖面內，上述突出部包含出現於上述第1剖面而未出現於上述第2剖面之部位、及出現於上述第1剖面及上述第2剖面兩者之部位。Further, another aspect of the present invention is a sample container having a hole capable of storing a liquid in an inner space of an upper opening, and in order to achieve the above first object, a bottom portion of the hole is provided facing the inner wall of the hole upward. a protruding portion in which the horizontal cross section of the internal space at a position at which the opening from the opening of the internal space is vertically downward is defined as a first cross section in the sample container in the horizontal posture, which is the first When the horizontal cross section of the internal space at the second depth position is deeper than the depth, the second cross section is included in the first cross section, and the protruding portion includes the first cross section. a portion appearing in the second cross section and a portion appearing in both the first cross section and the second cross section.

若於對保持為水平姿勢之樣品容器之孔已注入液體之狀態下自上方目視觀察該孔，則液面之形狀表示此時之液面高度的孔之內部空間之剖面形狀。且，於如上述般構成之發明中，由於剖面形狀根據自孔之開口觀察之深度而變化，故自上方觀察時之液面之形狀根據當時之液面高度而變化。即，根據上述構成，根據自上方觀察孔時之液面形狀可簡易地判定儲存於內部空間之液體量。When the hole is visually observed from above in a state in which the hole of the sample container held in the horizontal posture has been injected, the shape of the liquid surface indicates the cross-sectional shape of the internal space of the hole at the liquid level at this time. Further, in the invention constructed as described above, since the cross-sectional shape changes depending on the depth observed from the opening of the hole, the shape of the liquid surface when viewed from above changes depending on the liquid level at that time. In other words, according to the above configuration, the amount of liquid stored in the internal space can be easily determined based on the liquid surface shape when the hole is viewed from above.

又，於本發明中，局部設置於孔側壁面之突出部可作為操作者***對孔注入液體之移液管等器具之前端時之位置基準而發揮功能。即，藉由將用於對孔注入液體之器具之前端抵接於突出部，容易相對於孔進行器具之定位。因此，可大幅提高操作者操作之作業性及作業精度，且防止因器具之前端而使細胞等受損。即，達成上述第2目的。 [發明之效果]Further, in the present invention, the protruding portion partially provided on the side wall surface of the hole functions as a position reference when the operator inserts the front end of the device such as a pipette for injecting a liquid into the hole. That is, by abutting the front end of the device for injecting the liquid into the hole to the protruding portion, it is easy to position the device with respect to the hole. Therefore, the workability and the work accuracy of the operator's operation can be greatly improved, and the cells and the like can be prevented from being damaged by the front end of the device. That is, the above second object is achieved. [Effects of the Invention]

如上所述，根據本發明，孔之剖面形狀根據距離開口之深度而變化，且自上方觀察時之液面之形狀根據承載於孔之內部空間之液體之量而變化。因此，可藉由目視觀察液面之形狀簡易地判定孔內之液體量。又，藉由將用於操作液體之器具之前端相對於孔容易且準確地定位，可提高此種操作之作業性及作業精度。As described above, according to the present invention, the cross-sectional shape of the hole varies depending on the depth from the opening, and the shape of the liquid surface when viewed from above varies depending on the amount of liquid carried in the inner space of the hole. Therefore, the amount of liquid in the pore can be easily determined by visually observing the shape of the liquid surface. Further, by easily and accurately positioning the front end of the instrument for operating the liquid with respect to the hole, workability and work accuracy of such an operation can be improved.

圖1A及圖1B係表示作為本發明之樣品容器之一例之孔板之整體構造之圖。更詳細而言，圖1A係孔板10之俯視圖，又，圖1B係該孔板10之立體圖。該孔板10具有於例如塑膠製之板狀基材11，以固定間距規則地呈二維矩陣配置有複數個孔W之構造。各孔W具有上表面開口且底面封閉之大致圓筒形狀(或，水平截面面積朝底面遞減之帶錐形之圓錐台形狀)。此處，例示設置有12*8(=96)個孔W之孔板10，但設置於1個平板之孔之個數為任意。1A and 1B are views showing the entire structure of an orifice plate as an example of a sample container of the present invention. In more detail, FIG. 1A is a plan view of the orifice plate 10, and FIG. 1B is a perspective view of the orifice plate 10. The orifice plate 10 has a structure in which a plurality of holes W are regularly arranged in a two-dimensional matrix at a fixed pitch, for example, in a plate-like base material 11 made of plastic. Each of the holes W has a substantially cylindrical shape in which the upper surface is open and the bottom surface is closed (or a tapered truncated cone shape in which the horizontal cross-sectional area decreases toward the bottom surface). Here, the orifice plate 10 provided with 12*8 (=96) holes W is exemplified, but the number of holes provided in one plate is arbitrary.

孔板10以其上表面10a呈水平般之姿勢，於以各孔W之側壁面及底面包圍之內部空間可承載液體。例如，於醫學或生化學之領域，為了於注入孔W之培養液中培養細胞等而製作樣品，可較佳地應用該孔板10。圖1A及圖1B表示孔板10保持水平姿勢之狀態，XY面表示水平面，+Z方向表示鉛直向上之方向。The orifice plate 10 has a horizontal posture with its upper surface 10a, and the inner space surrounded by the side wall surface and the bottom surface of each of the holes W can carry liquid. For example, in the field of medicine or biochemistry, the orifice plate 10 can be preferably used in order to prepare a sample for culturing cells or the like in a culture solution in which a well W is injected. 1A and 1B show a state in which the orifice plate 10 is maintained in a horizontal posture, the XY plane represents a horizontal plane, and the +Z direction represents a direction of a vertical upward direction.

各孔W之底面亦可與基材11一體地形成。又，亦可藉由利用例如樹脂製之片體將在Z方向(鉛直方向)設置於基材11之貫通孔之底部堵住而構成孔底面。於藉由培養而製作之樣品被供於顯微鏡觀察或顯微攝像等之情形時，較理想為底面由相對於觀察時之照明光為透明之材料構成。The bottom surface of each of the holes W may be formed integrally with the substrate 11. Further, the bottom surface of the hole may be formed by blocking the bottom portion of the through hole provided in the Z direction (vertical direction) in the Z direction (for example, a resin). When the sample produced by the culture is supplied to a microscope or a microscopic image or the like, it is preferable that the bottom surface is made of a material transparent to the illumination light at the time of observation.

繼而，對孔W之內部構造進行說明。目前為止被製品化之一般之孔板中之各孔之內部空間規定為由水平剖面為圓形或矩形等相對簡單之形狀之側壁面與大致平坦之底面包圍之空間。因此，自上方觀察孔時，難以判定液面之高度。尤其於二維排列有多個孔之孔板中，亦無法自橫向觀察各孔之液面。因此，難以根據目視推斷承載於各孔W之液體之量。Next, the internal structure of the hole W will be described. The internal space of each of the holes in the conventional orifice plate which has been produced so far is defined as a space surrounded by a side wall surface of a relatively simple shape such as a circular or rectangular horizontal section and a substantially flat bottom surface. Therefore, when the hole is observed from above, it is difficult to determine the height of the liquid surface. In particular, in an orifice plate in which a plurality of holes are arranged in two dimensions, it is also impossible to observe the liquid level of each hole from the lateral direction. Therefore, it is difficult to visually infer the amount of liquid carried in each of the holes W.

例如，於以細胞等之培養為目的，將於孔W已注入培養液之孔板10放置於恆溫、恆濕之恆溫箱內之情形時，培養液隨著時間推移而蒸發。藉此，可能有培養液中之成分濃度變得過高，培養環境變得不合適之情形。又，於培養之樣品供光學觀察之情形時，可能會因液面下降導致液面之彎月面引起之透鏡效果變得顯著，對觀察造成障礙。For example, in the case of culturing a cell or the like, the culture solution is evaporated over time when the well plate 10 into which the culture solution is injected is placed in a constant temperature and constant humidity oven. As a result, there is a possibility that the concentration of the components in the culture solution becomes too high and the culture environment becomes unsuitable. Further, when the cultured sample is used for optical observation, the lens effect caused by the meniscus of the liquid surface may become conspicuous due to the drop in the liquid level, which may cause an obstacle to observation.

為了將此種問題防患於未然，必須隨時評估承載於各孔W之液體量且根據需要進行補充液體。然而，如上所述，於一般之孔板中難以藉由目視判定各孔之液面高度。因此，無法簡單地進行液量之評估。以下，針對可藉由自上方之目視簡單地判定孔W內之液量之孔W之內部構造之若干例進行說明。In order to prevent such problems from occurring, it is necessary to evaluate the amount of liquid carried in each well W at any time and to replenish the liquid as needed. However, as described above, it is difficult to visually determine the liquid level of each hole in a general orifice plate. Therefore, the evaluation of the liquid amount cannot be simply performed. Hereinafter, a description will be given of a few examples of the internal structure of the hole W which can be easily determined by the visual observation from the upper side.

圖2係表示具有本發明之內部構造之孔之第1例之圖。於圖中(a)欄為模式性表示孔之內部構造之透視圖。為了清楚地表示內部構造，藉由虛線表示相當於孔之開口、側壁面及底面之周緣之棱線。又，(b)欄為自上方觀察未注入液體之孔時之圖，(c)欄為以(a)欄所示之一點鏈線切開孔側壁面時之展開圖。Fig. 2 is a view showing a first example of a hole having the internal structure of the present invention. Column (a) in the figure is a perspective view schematically showing the internal structure of the hole. In order to clearly show the internal structure, the ridge line corresponding to the periphery of the opening, the side wall surface, and the bottom surface of the hole is indicated by a broken line. Further, the column (b) is a view when the hole in which the liquid is not injected is observed from above, and the column (c) is a developed view when the side wall surface of the hole is cut by the one-point chain line shown in the column (a).

又，(d)至(f)欄係表示液量之不同引起之孔之外觀不同的情況，於各欄中左圖為自側方觀察孔時之圖，中央之圖為以液面為剖面時之孔之水平剖視圖，右圖為自上方觀察孔時之液面之形狀之圖。分別為(d)欄表示液量相對較多之情形，(e)欄表示液量較(d)欄所示之情形更少之情形，(f)欄表示液量較(e)欄所示之情形進而更少之情形。Further, the columns (d) to (f) indicate that the appearance of the holes differs depending on the amount of liquid, and the left figure in each column is a view from the side observation hole, and the middle figure is a liquid surface section. The horizontal cross-sectional view of the hole at the time, and the right picture shows the shape of the liquid surface when the hole is observed from above. Column (d) indicates that the amount of liquid is relatively large, column (e) indicates that the amount of liquid is less than that shown in column (d), and column (f) indicates that the amount of liquid is as shown in column (e). The situation is then less.

再者，關於上述之(a)至(f)各欄之內容之說明亦同樣適用於以下之圖3至圖9之各者。Furthermore, the description of the contents of the respective columns (a) to (f) above applies equally to each of FIGS. 3 to 9 below.

圖2所示之第1例之孔W1可將液體儲存於上部成為開口面111且以大致圓筒形之側壁面112與大致平坦之底面113包圍之內部空間SP。於該孔W1中，設置有自底面113向上方且沿側壁面112呈柱狀延伸設置之3個突出部位114、115、116。突出部位114、115、116與側壁面112相接，因此，成為各突出部位114、115、116自側壁面112向孔內部空間SP之中央部呈凸條狀突出之構造體。再者，各突出部位114、115、116之水平剖面形狀於此處為矩形，但亦可為任意之形狀。The hole W1 of the first example shown in FIG. 2 can store the liquid in the internal space SP which is the upper surface of the opening surface 111 and which is surrounded by the substantially cylindrical side wall surface 112 and the substantially flat bottom surface 113. In the hole W1, three protruding portions 114, 115, and 116 extending upward from the bottom surface 113 and extending in a columnar shape along the side wall surface 112 are provided. Since the protruding portions 114, 115, and 116 are in contact with the side wall surface 112, the protruding portions 114, 115, and 116 are formed in a convex shape from the side wall surface 112 toward the central portion of the hole internal space SP. Further, the horizontal cross-sectional shape of each of the protruding portions 114, 115, and 116 is rectangular here, but may be any shape.

如(b)欄所示，若自上方觀察孔W1，則成為於開口面111之內部出現各突出部位114、115、116之頂面與底面113之狀態。如(a)欄及(c)欄所示，以底面113為基準時之鉛直方向高度為突出部位114最高，突出部位115次之，突出部位116最低。因此，於自開口面111沿鉛直方向朝下之深度方向上，突出部位114之頂面為最淺之位置，突出部位115之頂面次之，突出部位116之頂面為最深之位置。As shown in the column (b), when the hole W1 is viewed from above, the top surface and the bottom surface 113 of each of the protruding portions 114, 115, and 116 are formed inside the opening surface 111. As shown in the columns (a) and (c), the height in the vertical direction when the bottom surface 113 is the reference is the highest in the protruding portion 114, the protruding portion 115 is the second, and the protruding portion 116 is the lowest. Therefore, in the depth direction from the opening surface 111 in the vertical direction, the top surface of the protruding portion 114 is the shallowest position, the top surface of the protruding portion 115 is next, and the top surface of the protruding portion 116 is the deepest position.

認為存在如下情形：於此種構造之孔W1注入量相對較多之液體，如(d)欄所示，突出部位114之頂部露出於較液面S更上方，另一方面，突出部位115、116之頂部位於較液面S更下方。再者，實際上液面形成彎月面，但因(d)至(f)欄之左圖所示之液面S表示與孔側壁面112相接之部分之高度，故顯示為水平直線。相當於此時之液面高度之水平剖面中之孔W1之剖視圖為(d)欄中央之圖，如該圖所示，此時之剖面成為被突出部位114切除側壁面112所形成之大致圓形之外周部之一部分所得之形狀。It is considered that there is a case where the hole W1 of such a configuration is injected with a relatively large amount of liquid, as shown in the column (d), the top of the protruding portion 114 is exposed above the liquid level S, and on the other hand, the protruding portion 115, The top of 116 is located below the liquid level S. Further, the liquid surface actually forms a meniscus, but since the liquid surface S shown in the left diagram of the columns (d) to (f) indicates the height of the portion in contact with the hole side wall surface 112, it is shown as a horizontal straight line. A cross-sectional view of the hole W1 in the horizontal section corresponding to the liquid level at this time is a view in the center of the column (d). As shown in the figure, the cross section at this time becomes a substantially circle formed by the protruding portion 114 cutting the side wall surface 112. The shape obtained by one part of the outer circumference.

又，若自上方經由開口面111觀察此時之液面S，則如(d)欄右圖所示，成為僅突出部位114自大致圓形之液面S露出，其他突出部位115、116隱沒於液中之狀態。換言之，若自上方觀察之液面S為(d)欄右圖所示之狀態，則當時之液量可為如僅露出突出部位114般之相對較多者。Further, when the liquid surface S at this time is observed from above through the opening surface 111, as shown in the right diagram of the column (d), only the protruding portion 114 is exposed from the substantially circular liquid surface S, and the other protruding portions 115, 116 are hidden. In the state of the liquid. In other words, if the liquid level S observed from above is in the state shown in the right diagram of the column (d), the amount of liquid at that time may be relatively large as if only the protruding portion 114 is exposed.

另一方面，如(e)欄所示，於液量為除了突出部位114以外突出部位115之頂部亦自液面S露出之情形時，該液面高度之水平剖面中之孔W1之剖面之形狀與(d)欄所示者不同。具體而言，如(e)欄中央之圖所示，成為自(d)欄中央所示之剖面進而增加有與突出部位115對應之缺口之形狀。若自上方觀察此時之液面，則如(e)欄右圖所示，成為自液面S露出突出部位114、115之狀態。On the other hand, as shown in the column (e), when the liquid amount is such that the top portion of the protruding portion 115 is exposed from the liquid surface S except for the protruding portion 114, the cross section of the hole W1 in the horizontal cross section of the liquid level height is The shape is different from that shown in the column (d). Specifically, as shown in the middle view of the column (e), the cross section shown in the center of the column (d) is further increased in shape corresponding to the notch corresponding to the protruding portion 115. When the liquid level at this time is observed from above, as shown in the right diagram of the column (e), the liquid-surface S is exposed to the protruding portions 114 and 115.

於液量進而變少，如(f)欄左圖所示般3個突出部位114、115、116全部之頂部均自液面S露出之狀態下，與此對應，該液面高度之孔W1之剖面形狀成為進而增加與突出部位116對應之缺口之形狀。又，自上方觀察液面S時之狀態亦成為3個突出部位114、115、116全部自液面S露出者。The liquid amount is further reduced, and as shown in the left diagram of the column (f), the tops of all the three protruding portions 114, 115, and 116 are exposed from the liquid surface S, and correspondingly, the liquid level height hole W1 The cross-sectional shape is such that the shape of the notch corresponding to the protruding portion 116 is increased. Further, the state in which the liquid surface S is observed from above is also such that all of the three protruding portions 114, 115, and 116 are exposed from the liquid surface S.

如此，藉由於孔W1之側壁部112設置距離底面113之高度彼此不同(即距離開口面111之深度彼此不同)之突出部位114、115、116，液面高度之孔內部空間SP之剖面形狀會根據液量而變化。伴隨於此，自上方觀察液面S時之外觀亦變化。利用該點，能夠藉由自上方觀察液面S而評估孔W1內之液量。Thus, since the side wall portion 112 of the hole W1 is provided with the protruding portions 114, 115, 116 which are different from each other in height from the bottom surface 113 (i.e., the depths of the opening faces 111 are different from each other), the cross-sectional shape of the hole internal space SP of the liquid level height is It varies depending on the amount of liquid. Along with this, the appearance when the liquid surface S is observed from above also changes. From this point, the amount of liquid in the hole W1 can be evaluated by observing the liquid surface S from above.

例如，只要將突出部位114之高度設定為與應由孔W1承載之液體量之上限值對應之液面高度，將突出部位116之高度設定為與應由孔W1承載之液體量之下限值對應之液面高度，則可知如下情況。即，自上方觀察液面時，只要突出部位均未露出則液體量過剩。另一方面，若所有突出部位均露出則液體量過少。至少1個突出部位露出，且至少1個突出部位隱於液體中之狀態表示孔W1內之液量處於適當之範圍內之狀態。For example, if the height of the protruding portion 114 is set to the liquid level corresponding to the upper limit of the amount of liquid to be carried by the hole W1, the height of the protruding portion 116 is set to be the lower limit of the amount of liquid to be carried by the hole W1. The liquid level height corresponding to the value is as follows. That is, when the liquid level is observed from above, the amount of liquid is excessive as long as the protruding portions are not exposed. On the other hand, if all the protruding portions are exposed, the amount of liquid is too small. At least one protruding portion is exposed, and a state in which at least one protruding portion is hidden in the liquid indicates a state in which the amount of liquid in the hole W1 is within an appropriate range.

然而，若僅設置對應於液量之上限及下限之2個突出部位，則無法掌握液量處於適當範圍內之哪個位置。因此，在突出部位116自液面露出之前無法檢測液量之減少，而無法達成瞭解液體補充之時期之目的。However, if only two protruding portions corresponding to the upper limit and the lower limit of the liquid amount are provided, it is impossible to grasp which position of the liquid amount is within the appropriate range. Therefore, it is impossible to detect the decrease in the amount of liquid before the protruding portion 116 is exposed from the liquid surface, and the purpose of understanding the period of liquid replenishment cannot be achieved.

因此，藉由設置突出部位114之高度與突出部位116之高度之間的中間高度之突出部位115，能夠判斷液體為靠近上限、下限哪個狀態。即，自上方觀察液面時，若僅突出部位114露出，則可確保高度為液面至少超過突出部位115之高度之程度之液量。另一方面，若突出部位114與突出部位115露出，則表示液量更靠近下限。只要於與成為補充之標準之液量時之液面高度對應之位置設置突出部位115之頂部，則能夠以確認到突出部位115之露出來判斷需要進行液體之補充。相反，若突出部位115未露出，則可判斷無需進行補充。Therefore, by providing the protruding portion 115 of the intermediate height between the height of the protruding portion 114 and the height of the protruding portion 116, it is possible to determine which state the liquid is close to the upper limit and the lower limit. That is, when the liquid surface is observed from above, if only the protruding portion 114 is exposed, it is possible to ensure the liquid level to such an extent that the liquid surface at least exceeds the height of the protruding portion 115. On the other hand, if the protruding portion 114 and the protruding portion 115 are exposed, it means that the liquid amount is closer to the lower limit. When the top of the protruding portion 115 is provided at a position corresponding to the liquid level at which the liquid amount of the standard is added, it is possible to confirm that the liquid needs to be replenished by confirming the exposure of the protruding portion 115. On the contrary, if the protruding portion 115 is not exposed, it can be judged that no replenishment is required.

如此，於第1例之孔W1中，設置有沿側壁面112距離開口面111之深度彼此不同之3個突出部位114、115、116。藉此，能夠藉由自上方觀察液面S而掌握伴隨液量變化之液面S之高度之變化。只要設置至少2處之突出部位即可表示液量之上限及下限。若需要更多階段地判定液量，只要進而增多突出部位之設置數量即可。由於根據自液面露出之突出部位之數量表示液量，故無需專業知識即可進行判斷。As described above, in the hole W1 of the first example, three protruding portions 114, 115, and 116 having different depths from the opening surface 111 along the side wall surface 112 are provided. Thereby, the change in the height of the liquid surface S accompanying the change in the liquid amount can be grasped by observing the liquid surface S from above. The upper and lower limits of the amount of liquid can be expressed by providing at least two protruding portions. If it is necessary to determine the amount of liquid in more stages, it is only necessary to increase the number of the protruding parts. Since the amount of liquid is expressed in terms of the number of protruding portions exposed from the liquid surface, it is judged without special knowledge.

再者，於先前之孔板中，孔內部空間之水平剖面隨著接近底面而變小，側壁面成為所謂之錐形狀。於此種構造之孔中，自上方觀察液面時之液面之形狀並未改變，但其面積隨著液面之高度而變化，該情形可成為用於判斷液量之資訊。然而，亦有僅大小之變化以目視觀察難以判斷，而需要與某種基準對比之情形。如上述例所示，若使內部空間之水平剖面之外周形狀根據深度而變化為不相似之形狀，則不具備詳盡之知識之使用者亦可容易地評估液體量。突出部位沿側壁面112而設置，故對孔中央部之細胞等之培養及觀察影響較少。Further, in the prior orifice plate, the horizontal section of the inner space of the hole becomes smaller as it approaches the bottom surface, and the side wall surface becomes a so-called tapered shape. In the hole of such a configuration, the shape of the liquid surface when the liquid level is observed from above does not change, but the area thereof changes depending on the height of the liquid surface, and this case can be used for judging the amount of liquid. However, there are cases where only the change in size is difficult to judge by visual observation, and it is necessary to compare with a certain benchmark. As shown in the above example, if the outer peripheral shape of the horizontal section of the internal space is changed to a dissimilar shape depending on the depth, the user who does not have detailed knowledge can easily evaluate the amount of liquid. Since the protruding portion is provided along the side wall surface 112, there is little influence on the culture and observation of cells or the like in the central portion of the hole.

又，為了藉由自上方之觀察能夠檢測液面高度之變化引起之孔內部空間之剖面形狀之變化，必須於較液面更上方不存在遮蔽表示剖面形狀之液面並阻礙自上方之視認之構造物。因此，只要以孔內部空間SP之任意深度位置處之一剖面(深度0之剖面、即包含開口面111)於俯視下完全包含較其更深之位置處之任意剖面的方式設定各部之形狀即可。Moreover, in order to detect the change in the cross-sectional shape of the internal space of the hole caused by the change in the liquid level from the top, it is necessary to prevent the liquid level indicating the cross-sectional shape from being obscured above the liquid surface and hinder the visual recognition from above. Structure. Therefore, the shape of each part can be set such that one of the cross-sections at any depth position of the internal space SP of the hole (the cross-section of the depth 0, that is, the opening surface 111) is completely included in the plan view and includes any cross-section at a position deeper than the deeper position. .

於圖2之例中，將各突出部位114、115、116設為自底面113向上延伸之剖面形狀為固定之柱狀體。因此，滿足上述之必要條件。又，只要滿足上述之必要條件，則可容許各部之形狀之變更。例如，側壁面112亦可具有越靠近底面113而水平剖面越小之錐形狀。又，例如，各突出部位114、115、116亦可為下部之截面面積較上部更大。In the example of FIG. 2, each of the protruding portions 114, 115, and 116 is a columnar body having a cross-sectional shape extending upward from the bottom surface 113. Therefore, the above-mentioned necessary conditions are satisfied. Further, as long as the above-described requirements are satisfied, the shape of each part can be changed. For example, the side wall surface 112 may have a tapered shape that is closer to the bottom surface 113 and has a smaller horizontal section. Further, for example, each of the protruding portions 114, 115, and 116 may have a lower cross-sectional area larger than that of the upper portion.

以下，依次說明具有本發明之內部構造之孔之其他若干例。上述之例與以下之各例中突出部位之形狀不同，因此於液面出現之形狀變化之態樣不同。然而，欲以液面之形狀變化表示液量之基本原理為共通。因此，就各例中之構造或功能與上述例共通之方面省略詳細之說明，以與上述例之不同點為中心進行說明。Hereinafter, other examples of the holes having the internal structure of the present invention will be sequentially described. The above examples are different from the shapes of the protruding portions in the following examples, and therefore the shape changes in the liquid surface are different. However, the basic principle of expressing the amount of liquid in order to change the shape of the liquid surface is common. Therefore, the detailed description of the structure or function in each example is common to the above-described examples, and the differences from the above-described examples will be mainly described.

圖3係表示具有本發明之內部構造之孔之第2例之圖。如上所述，圖3各欄之外觀與圖2相同。於圖3所示之第2例之孔W2中，替代於第1例之孔W1中自側壁面112向內突出設置成凸條狀之突出部位114、115、116，而具有自孔W2之底面123沿側壁面122向上突出而設置之圓柱狀之突出部位124、125、126。再者，突出部位之剖面形狀並不限定於圓形而為任意。又，各突出部位124、125、126可與孔W2之側壁面122相接，亦可與側壁面122分離。Fig. 3 is a view showing a second example of a hole having the internal structure of the present invention. As described above, the appearance of each column of Fig. 3 is the same as that of Fig. 2. In the hole W2 of the second example shown in FIG. 3, instead of the protruding portion 114, 115, 116 which is formed in a convex strip shape from the side wall surface 112 in the hole W1 of the first example, it has the self-hole W2. The bottom surface 123 protrudes upward along the side wall surface 122 to provide cylindrical projections 124, 125, 126. Further, the cross-sectional shape of the protruding portion is not limited to a circular shape and is arbitrary. Further, each of the protruding portions 124, 125, 126 may be in contact with the side wall surface 122 of the hole W2 or may be separated from the side wall surface 122.

自上方觀察此種構造之孔W2時，如(b)欄所示，成為於對應於開口面121之圓形區域內包含有突出部位124、125、126之頂面之狀態。又，若為各突出部位124、125、126與孔W2之側壁面122分離之狀態，則如(d)欄至(f)欄所示，即使於孔內部空間SP中液面S之高度變化，液面高度之位置處之內部空間SP之剖面之外周形狀(該情形時為圓形)亦不變。包含於外周形狀中之相當於各突出部位之剖面之圓形之個數根據液面高度而變化。When the hole W2 of such a structure is observed from above, as shown in the column (b), the top surface of the protruding portions 124, 125, and 126 is included in the circular region corresponding to the opening surface 121. Further, if the protruding portions 124, 125, and 126 are separated from the side wall surface 122 of the hole W2, the height of the liquid surface S in the internal space SP of the hole is changed as shown in the column (d) to (f). The outer peripheral shape of the cross section of the internal space SP at the position of the liquid level height (in this case, a circular shape) also does not change. The number of circles corresponding to the cross section of each protruding portion included in the outer peripheral shape changes depending on the liquid level.

具體而言，於液面S處於相對較高之位置之(d)欄所示之狀態下，頂部位於較液面S為高之位置之突出部位124之剖面包含於表示外周形狀之圓中。又，於液面S處於相對較低之位置之(e)欄所示之狀態下，頂部位於較液面S為高之位置之突出部位124、125之剖面包含於表示外周形狀之圓中。另一方面，於液面S處於最低之位置之(f)欄所示之狀態下，由於所有的突出部位之頂部位於較液面S為高之位置，故各突出部位124、125、126之剖面包含於表示外周形狀之圓中。Specifically, in a state shown in the column (d) where the liquid surface S is at a relatively high position, the cross section of the protruding portion 124 whose top portion is at a position higher than the liquid surface S is included in a circle indicating the outer peripheral shape. Further, in a state shown in the column (e) where the liquid surface S is at a relatively low position, the cross section of the protruding portions 124 and 125 whose top portion is at a position higher than the liquid surface S is included in a circle indicating the outer peripheral shape. On the other hand, in the state shown in the column (f) where the liquid level S is at the lowest position, since the tops of all the protruding portions are located at a position higher than the liquid level S, the respective protruding portions 124, 125, 126 The section is included in a circle representing the shape of the outer circumference.

伴隨著此種剖面形狀之變化，於經由開口121自上方觀察液面S時，如(d)～(f)欄之右圖所示，自液面露出之突出部位之個數根據液面S之高度而變化。因此，藉由適當設定各突出部位124、125、126之頂部之高度，與第1例之孔W1同樣地，藉由液面S之目視，可獲得液量之上限及下限、及用於掌握需要補充之時刻等之資訊等。於上述之先前技術中，構成為突起部設置於底面中央部而積極地涉入細胞之培養，但於本例中，由於突出部位124等僅設置於孔側壁面122之附近，故底面形狀為任意，例如若設為大致平坦之形狀則對細胞等之培養及其觀察無影響。亦可將本發明之突出部位與如上述先前技術之突起部加以組合實施。With the change of the cross-sectional shape, when the liquid surface S is viewed from above through the opening 121, as shown in the right diagram of the columns (d) to (f), the number of protruding portions exposed from the liquid surface is based on the liquid surface S. The height changes. Therefore, by appropriately setting the heights of the tops of the respective protruding portions 124, 125, and 126, the upper limit and the lower limit of the liquid amount can be obtained by visual observation of the liquid surface S, and the same can be grasped for the same as the hole W1 of the first example. Information such as the moment of replenishment. In the above-described prior art, the protrusion is provided at the central portion of the bottom surface and is actively involved in the culture of the cells. However, in this example, since the protruding portion 124 or the like is provided only in the vicinity of the hole side wall surface 122, the shape of the bottom surface is Any, for example, a substantially flat shape has no effect on the culture and observation of cells or the like. The protruding portion of the present invention can also be combined with the protrusion of the prior art as described above.

圖4係表示具有本發明之內部構造之孔之第3例之圖。於第3例之孔W3中，取代於第1例之孔W1中自側壁面112向孔內部空間SP之中央部側突出之突出部位114、115、116，而如(a)欄所示，設置自側壁面132向外突出之突出部位134、135、136。若自外部空間觀察，則突出部位134、135、136亦可謂具有自側壁面132後退之面之槽狀之凹部。該等突出部位之水平剖面形狀為任意。Fig. 4 is a view showing a third example of a hole having the internal structure of the present invention. In the hole W3 of the third example, instead of the protruding portion 114, 115, 116 protruding from the side wall surface 112 toward the central portion side of the hole internal space SP in the hole W1 of the first example, as shown in the column (a), Projecting portions 134, 135, 136 projecting outward from the side wall surface 132 are provided. When viewed from the external space, the protruding portions 134, 135, and 136 may be groove-shaped recesses having a surface receding from the side wall surface 132. The horizontal cross-sectional shape of the protruding portions is arbitrary.

如上所述，基於一水平剖面中之孔內部空間SP之剖面包含較其更深之位置處之全部剖面的要求，於該例中，各突出部位134、135、136自於孔板上部開口之開口面131向下延伸設置。各突出部位134、135、136之底部之高度(即距離開口面131之深度)彼此不同，但皆於較孔底面133之位置更淺之位置具有底部。該等底部與第1例之孔W1中之各突出部位114、115、116之頂部同樣地發揮功能。As described above, the profile based on the internal space SP of the hole in a horizontal section includes the requirements of all the sections at the deeper position, in this example, the opening of each of the protruding portions 134, 135, 136 from the upper opening of the hole. The face 131 extends downward. The height of the bottom of each of the protruding portions 134, 135, 136 (i.e., the depth from the opening surface 131) is different from each other, but both have a bottom portion at a position shallower than the position of the bottom surface 133 of the hole. These bottoms function in the same manner as the tops of the respective protruding portions 114, 115, and 116 in the hole W1 of the first example.

即，於液體未進入孔W3之狀態下，如(b)欄所示，經由開口131自上方觀察孔W3時，可看到孔底面133與各突出部位134、135、136之底部。另一方面，例如，如(d)欄所示，於突出部位135、136之底部位於較液面S更下方、突出部位134之底部位於較液面S更上方之液量時，該液面高度之孔內部空間SP之剖面具有對應於側壁面132之圓中對應於突出部位135、136之位置向外側擴展之形狀。因此，經由開口面131自上方觀察液面S時，成為突出部位135、136之底部為隱沒於液體中，僅突出部位134之底部露出之狀態。That is, when the liquid does not enter the hole W3, as shown in the column (b), when the hole W3 is viewed from above through the opening 131, the bottom surface 133 of the hole and the bottom of each of the protruding portions 134, 135, and 136 can be seen. On the other hand, for example, as shown in the column (d), when the bottom of the protruding portions 135, 136 is located below the liquid level S and the bottom of the protruding portion 134 is located above the liquid level S, the liquid level The cross section of the height inner space SP has a shape that expands outward in a position corresponding to the protruding portions 135, 136 of the circle corresponding to the side wall surface 132. Therefore, when the liquid surface S is viewed from above through the opening surface 131, the bottom portions of the protruding portions 135 and 136 are hidden in the liquid, and only the bottom portion of the protruding portion 134 is exposed.

如(e)欄及(f)欄所示，若液面S之高度變化，則孔W3之剖面之外周形狀隨之變化，自上方觀察之液面S之形狀亦變化。即，隨著液量變少，突出部位135之底部、突出部位136之底部依次自液面S露出。因此，與第1例同樣地，根據自上方觀察之各突出部位之露出之狀況，可獲得液面之高度、即液量相關之資訊。As shown in the columns (e) and (f), if the height of the liquid surface S changes, the outer peripheral shape of the cross section of the hole W3 changes, and the shape of the liquid surface S as viewed from above also changes. That is, as the amount of liquid becomes smaller, the bottom of the protruding portion 135 and the bottom of the protruding portion 136 are sequentially exposed from the liquid surface S. Therefore, in the same manner as in the first example, the information on the height of the liquid surface, that is, the liquid amount can be obtained based on the state of exposure of the respective protruding portions viewed from above.

如此，於突出部位自側壁面向外設置之構成中，液面越下降，出現於該液面高度之剖面之突出部位變得越少。但是，成為未出現於剖面之部分之底部自液面S露出之狀態，故自上方之觀察可容易地視認。As described above, in the configuration in which the protruding portion is provided outward from the side wall surface, the lower the liquid surface, the less the protruding portion of the cross section appearing at the liquid level height becomes. However, since the bottom portion of the cross-section is not exposed from the liquid surface S, it can be easily observed from the top.

圖5係表示具有本發明之內部構造之孔之第4例之圖。於第4例之孔W4中，頂部之高度多階段地變化之單一之突出部位140自孔W4之側壁面142向孔內部空間SP之中央部側突出而設置，以取代於第1例中隔離配置之彼此高度不同之複數個突出部位。即，突出部位140具有階梯狀之上部構造，該階梯狀之上部構造包含在內部空間SP之內部位於最高位置之平面部144、位於較其更低之位置之平面部145、及位於進而更低之位置之平面部146。Fig. 5 is a view showing a fourth example of a hole having the internal structure of the present invention. In the hole W4 of the fourth example, a single protruding portion 140 in which the height of the top portion is changed in stages is protruded from the side wall surface 142 of the hole W4 toward the central portion side of the hole inner space SP, instead of being isolated in the first example. A plurality of protruding portions that are different in height from each other. That is, the protruding portion 140 has a stepped upper portion structure including a flat portion 144 located at the highest position inside the internal space SP, a flat portion 145 located at a lower position, and even lower The planar portion 146 of the position.

因此，與上述原理相同，伴隨著液面S之減少，突出部位140之各平面部中露出之部分變多，藉此，自開口面141觀察液面S時之各平面部144、145、146之露出狀況變化。因此，與第1例同樣地，根據自上方觀察之各平面部之露出狀況，可獲得液面S之高度即關於液量之資訊。Therefore, similarly to the above-described principle, as the liquid surface S is reduced, the portion of each of the flat portions of the protruding portion 140 is exposed, whereby the flat portions 144, 145, and 146 when the liquid surface S is observed from the opening surface 141 are formed. The exposed situation changes. Therefore, similarly to the first example, the height of the liquid surface S, that is, the information on the liquid amount can be obtained from the exposure state of each flat portion viewed from above.

圖6係表示具有本發明之內部構造之孔之第5例之圖。於第4例中設置有高度依序變化之階梯狀之平面部。於第5例之孔W5中，取代於其，以隔著最低之平面部155之方式具有最高之平面部154及次高之平面部156之突出部位150自側壁面152向內突出而設置。Fig. 6 is a view showing a fifth example of the hole having the internal structure of the present invention. In the fourth example, a stepped planar portion whose height is sequentially changed is provided. In the hole W5 of the fifth example, the protruding portion 150 having the highest flat portion 154 and the second highest flat portion 156 so as to protrude inward from the side wall surface 152 with the lowest flat portion 155 interposed therebetween is provided.

於該例中，如(e)欄所示，經由開口面151觀察時，液面S之高度為中間位置時自液面S露出之平面部154、156看上去相互隔離。因此，就與(d)欄及(f)欄所示之狀態之識別性之方面而言，較圖5所示之第4例有所提高。In this example, as seen in the column (e), when viewed from the opening surface 151, the flat portions 154, 156 exposed from the liquid surface S when the height of the liquid surface S is the intermediate position appear to be isolated from each other. Therefore, the identification of the state shown in the columns (d) and (f) is improved as compared with the fourth example shown in FIG.

於第4及第5例中，作為其變化例，亦可將突出部位設為自側壁面向外突出之構造。作為其一例，對作為第4例所對應之變化例之第6例之孔W6進行說明。In the fourth and fifth examples, as a modification, the protruding portion may be configured to protrude outward from the side wall surface. As an example, the hole W6 of the sixth example which is a modification example corresponding to the fourth example will be described.

圖7係表示具有本發明之內部構造之孔之第6例之圖。於第6例之孔W6中，具有階梯狀之底部之突出部位160自側壁面162向外突出而設置。即，突出部位160具有階梯狀之下部構造，該階梯狀之下部構造包含位於最高位置之平面部164、位於次高之平面部165及位於最低位置之底面部166。Fig. 7 is a view showing a sixth example of a hole having the internal structure of the present invention. In the hole W6 of the sixth example, the protruding portion 160 having the stepped bottom portion is provided to protrude outward from the side wall surface 162. That is, the protruding portion 160 has a stepped lower portion structure including a flat portion 164 at the highest position, a second highest flat portion 165, and a bottom portion 166 at the lowest position.

於該例中亦如(d)～(f)欄所示，孔W6之剖面之外周形狀根據液面S之高度而變化。伴隨於此，自開口面161觀察液面S時之液面S之形狀及各平面部164、165、166之露出狀況變化。因此，與上述各例同樣地，根據自上方觀察之各平面部之露出狀況，可獲得液面S之高度即關於液量之資訊。Also in this example, as shown in the columns (d) to (f), the outer peripheral shape of the cross section of the hole W6 changes depending on the height of the liquid surface S. Accordingly, the shape of the liquid surface S when the liquid surface S is observed from the opening surface 161 and the exposure state of each of the flat portions 164, 165, and 166 are changed. Therefore, similarly to the above-described respective examples, the height of the liquid surface S, that is, the information on the liquid amount can be obtained from the exposure state of each flat portion viewed from above.

圖8係表示具有本發明之內部構造之孔之第7例之圖。於第7例之孔W7中，替代上述第4例之階梯狀之上部構造，上表面之高度連續變化之突出部位170自孔W7之側壁面172向內部空間SP中央側突出而設置。具體而言，突出部位170之頂部成為小面積之平面部174。且，連接於平面部174之傾斜面部175成為沿高度連續變化至該傾斜面部175之後端部176為止之側壁面172之傾斜面，即，該面之法線具有鉛直向上之成分與水平方向成分之面。如(c)欄之實線、點線及虛線所示，接續於後端部176之部分之高度變化為任意。又，只要製造上不產生問題，平面部174亦可省略。Fig. 8 is a view showing a seventh example of a hole having the internal structure of the present invention. In the hole W7 of the seventh example, in place of the stepped upper portion structure of the fourth example, the protruding portion 170 whose height of the upper surface continuously changes protrudes from the side wall surface 172 of the hole W7 toward the center side of the internal space SP. Specifically, the top of the protruding portion 170 becomes a small-area planar portion 174. Further, the inclined surface portion 175 connected to the flat portion 174 becomes an inclined surface of the side wall surface 172 which continuously changes in height to the end portion 176 after the inclined surface portion 175, that is, the normal to the surface has a vertical upward component and a horizontal component The face. As indicated by the solid line, the dotted line, and the broken line in the column (c), the height change of the portion following the rear end portion 176 is arbitrary. Further, the flat portion 174 may be omitted as long as no problem occurs in manufacturing.

於具有此種構造之孔W7中，如(d)～(f)欄所示，自液面S露出之傾斜面部175之長度根據液面S之高度而增加。伴隨於此，突出部位170所對應之區域占剖面之面積增加。因此，經由開口面171觀察液面S時，伴隨著液量之減少，露出之傾斜面部175之長度變長。即，能夠將液面S之高度轉換為露出之傾斜面部175之長度表示。In the hole W7 having such a configuration, as shown in the columns (d) to (f), the length of the inclined surface portion 175 exposed from the liquid surface S increases in accordance with the height of the liquid surface S. Along with this, the area corresponding to the protruding portion 170 increases in the area of the cross section. Therefore, when the liquid surface S is observed through the opening surface 171, the length of the exposed inclined surface portion 175 becomes longer as the liquid amount decreases. That is, the height of the liquid surface S can be expressed as the length of the exposed inclined surface portion 175.

為了更簡單地獲得液量之上限及下限或表示液體之補充時刻之資訊，例如，如(f)欄所示，只要於開口171之周圍之基材上表面設置適當之標記Mk即可。標記Mk例如可藉由印刷、刻設等形成。於自上表面之觀察中，能夠基於伴隨著液量下降而延伸之傾斜面部175之前端部與標記Mk之位置關係，定量地顯示擔載之液體量。In order to more easily obtain the upper limit and the lower limit of the liquid amount or the information indicating the time of replenishment of the liquid, for example, as shown in the column (f), an appropriate mark Mk may be provided on the upper surface of the substrate around the opening 171. The mark Mk can be formed, for example, by printing, engraving, or the like. In the observation from the upper surface, the amount of liquid to be carried can be quantitatively displayed based on the positional relationship between the front end portion of the inclined surface portion 175 and the mark Mk which is extended with the decrease in the amount of liquid.

圖9係表示具有本發明之內部構造之孔之第8例之圖。於第8例之孔W8中，取代於上述第7例中向內突出之突出部位，而具有底部高度連續地變化之突出部位180自側壁面182向外突出之構造。此種構造亦與第7例同樣地，隨著液面S之下降，突出部位180之傾斜面部185中之露出之部分增加。隨之，自開口面181觀察時之液面S之形狀及突出部位180之露出狀況變化。Fig. 9 is a view showing an eighth example of a hole having the internal structure of the present invention. In the hole W8 of the eighth example, instead of the protruding portion which protrudes inward in the above-described seventh example, the protruding portion 180 having the bottom height continuously changes protrudes outward from the side wall surface 182. Also in this configuration, as in the seventh example, as the liquid surface S is lowered, the exposed portion of the inclined surface portion 185 of the protruding portion 180 is increased. Accordingly, the shape of the liquid surface S and the exposed state of the protruding portion 180 when viewed from the opening surface 181 change.

具體而言，如(d)欄所示，於液面S位於較高位置時僅傾斜面185之上端部184附近自液面S露出於上方，但伴隨著液面下降，傾斜面部185之露出部分增加。於自上方之觀察中，於液面S之外周部可觀察到之突出部位180逐漸延長。傾斜面部185之下端部186之位置可根據液面S掌握。因此，根據藉由自上方之觀察獲得之傾斜面部185之露出部分之前端至下端部186之距離，可直接掌握液量接近下限至何種程度。Specifically, as shown in the column (d), when the liquid surface S is at a higher position, only the vicinity of the upper end portion 184 of the inclined surface 185 is exposed above the liquid surface S, but the liquid surface is lowered, and the inclined surface portion 185 is exposed. Partially increased. In the observation from the top, the protruding portion 180 which is observed at the outer periphery of the liquid surface S is gradually elongated. The position of the lower end portion 186 of the inclined surface portion 185 can be grasped according to the liquid level S. Therefore, according to the distance from the front end to the lower end portion 186 of the exposed portion of the inclined surface portion 185 obtained from the above observation, it is possible to directly grasp the extent to which the liquid amount approaches the lower limit.

圖10係表示具有本發明之內部構造之孔之第9例之圖。更具體而言，圖10(a)係表示第9例之孔W9之內部構造之透視圖及表示孔W9之水平截面面積之變化之圖。又，圖10(b)係孔W9之俯視圖，圖10(c)係圖10(b)之A-A線剖視圖。Fig. 10 is a view showing a ninth example of a hole having the internal structure of the present invention. More specifically, Fig. 10(a) is a perspective view showing the internal structure of the hole W9 of the ninth example and a graph showing the change in the horizontal cross-sectional area of the hole W9. Further, Fig. 10(b) is a plan view of the hole W9, and Fig. 10(c) is a cross-sectional view taken along line A-A of Fig. 10(b).

於第9例之孔W9中，內部空間SP之垂直剖面之概略形狀與上述各例者不同。即，於該例之孔W9中，自開口面191至底面193之側壁面具有如下之構造。即，成為如下構造：於側壁面中，具有相對較大之水平截面面積之第1側壁面192與具有更小之水平截面面積之第2側壁面198經由水平截面面積向下急劇縮小之縮徑部位197而連結。In the hole W9 of the ninth example, the schematic shape of the vertical cross section of the internal space SP is different from those of the above examples. That is, in the hole W9 of this example, the side wall surface from the opening surface 191 to the bottom surface 193 has the following structure. In other words, in the side wall surface, the first side wall surface 192 having a relatively large horizontal cross-sectional area and the second side wall surface 198 having a smaller horizontal cross-sectional area are sharply reduced downward by the horizontal cross-sectional area. The part 197 is connected.

如圖10(a)右圖所示，孔內部空間SP之水平截面面積於縮徑部位197之前後急劇地變化。如該圖中虛線所示，側壁面192、193之至少一者可具有隨著深度增加而截面面積變小之錐形狀。於該情形時，可將截面面積相對於深度變化之變化率明顯大於其他深度之變化率之部分視為縮徑部位。As shown in the right diagram of Fig. 10(a), the horizontal cross-sectional area of the internal space SP of the hole changes sharply before and after the reduced diameter portion 197. As indicated by the broken line in the figure, at least one of the side wall faces 192, 193 may have a tapered shape in which the cross-sectional area becomes smaller as the depth increases. In this case, the portion where the rate of change of the cross-sectional area with respect to the depth change is significantly larger than the rate of change of the other depth can be regarded as the reduced diameter portion.

即，孔W9之內部空間SP成為由側壁面192規定之水平截面面積相對較大之第1空間SP1與由側壁面198規定之水平截面面積更小之第2空間SP2於上下方向連結之構造。於作為該連結部分之縮徑部位197中，孔W9之側壁面具有階差。此意味著縮徑部位197亦可謂作為側壁面之一部分之「階差部」。In other words, the internal space SP of the hole W9 has a structure in which the first space SP1 having a relatively large horizontal cross-sectional area defined by the side wall surface 192 and the second space SP2 having a smaller horizontal cross-sectional area defined by the side wall surface 198 are connected in the vertical direction. In the reduced diameter portion 197 as the joint portion, the side wall surface of the hole W9 has a step. This means that the reduced diameter portion 197 can also be referred to as a "step portion" which is a part of the side wall surface.

且，於較縮徑部位197更上方之側壁面192，設置有與上述之第1例(圖2)之突出部位114、115、116相同之構造及功能之突出部位194、195、196。再者，於圖10(a)右圖中，可忽視因突出部位導致之截面面積之變化。另一方面，於較縮徑部位197更下方，側壁面198形成單純之圓筒面。但，亦可設置成錐形。Further, the side wall surface 192 above the reduced diameter portion 197 is provided with protruding portions 194, 195, and 196 having the same structure and function as the protruding portions 114, 115, and 116 of the first example (Fig. 2) described above. Furthermore, in the right diagram of Fig. 10(a), the change in the cross-sectional area due to the protruding portion can be ignored. On the other hand, below the reduced diameter portion 197, the side wall surface 198 forms a simple cylindrical surface. However, it can also be set to be tapered.

圖11係例示承載於具有階差之孔之液體之拍攝的圖。考慮注入於孔W9之液體中之試料供拍攝之情形。再者，此處說明落射照明與透過拍攝之組合之例，但拍攝方法並不限定於此。例如，於孔W9之上方配置光源LS，使自光源LS向下照射之照明光L入射至孔W9。藉由利用攝像機構CM接收自孔底面193向下方透過之光，可拍攝孔W9內。Fig. 11 is a view showing the photographing of a liquid carried on a hole having a step. A sample injected into the liquid of the hole W9 is considered for photographing. Here, an example in which the combination of the epi-illumination and the transmission imaging is combined is described here, but the imaging method is not limited thereto. For example, the light source LS is disposed above the hole W9, and the illumination light L irradiated downward from the light source LS is incident on the hole W9. The inside of the hole W9 can be imaged by receiving the light transmitted downward from the bottom surface 193 of the hole by the image pickup mechanism CM.

此處，注入於孔W9之液體之液面S形成為與孔側壁面相接之部分突起之彎月面。因此，自上方入射至液面S之光L中之入射至靠近側壁面192之區域的光因液面之透鏡效果而向外側彎曲。藉此，若為例如內部空間SP之水平截面面積於深度方向上固定(或者具有連續且單調之微小變化)之先前之一般孔，則於底面之周緣部無法充分地入射照明光。其結果，有於獲得之圖像中亦導致底面之周緣部變暗之問題。Here, the liquid surface S of the liquid injected into the hole W9 is formed as a partially meniscus which is in contact with the side wall surface of the hole. Therefore, light incident on the region close to the side wall surface 192 from the light L incident from the upper surface to the liquid surface S is bent outward by the lens effect of the liquid surface. Therefore, for example, if the horizontal cross-sectional area of the internal space SP is fixed in the depth direction (or has a continuous and monotonous small change), the illumination light is not sufficiently incident on the peripheral portion of the bottom surface. As a result, there is a problem that the peripheral portion of the bottom surface is darkened in the obtained image.

另一方面，於具有上述構造之孔W9中，因彎月面而彎曲之光被縮徑部位197遮蔽。因此，於底面193中可獲得大致均一之照明條件。藉此，可拍攝直至周緣部仍足夠明亮之圖像。On the other hand, in the hole W9 having the above configuration, the light bent by the meniscus is shielded by the reduced diameter portion 197. Therefore, substantially uniform illumination conditions are obtained in the bottom surface 193. Thereby, it is possible to take an image until the peripheral portion is still bright enough.

為了獲得此種優點，液體必須注入至縮徑部位197更上方。因此，於該例之孔W9中，如上述般藉由自上方觀察可掌握液面S之高度之突出部位設置於較縮徑部位197更上方。藉由將利用突出部位產生之液面高度之視覺化應用於此種構造之孔，可藉由自上方之觀察而容易地判斷是否承載有適當量之液體。因此，可根據需要進行液體之補充。再者，此處，於具有階差之孔，組合有作為第1例說明之突出部位，但亦可組合其他例之構造。In order to achieve this advantage, the liquid must be injected above the reduced diameter portion 197. Therefore, in the hole W9 of this example, as described above, the protruding portion which can grasp the height of the liquid surface S as viewed from above is disposed above the reduced diameter portion 197. By visualizing the height of the liquid level generated by the protruding portion to the hole of such a configuration, it is possible to easily judge whether or not an appropriate amount of liquid is carried by observation from above. Therefore, the liquid can be replenished as needed. Here, in the hole having the step difference, the protruding portion described as the first example is combined, but the structure of another example may be combined.

於上述之各構造之孔W(W1～W9)中，相對於先前其剖面形狀為圓形或矩形等相對簡單之形狀，於剖面之輪廓之一部分設置向外側或內側突出之突出部位。藉此，因剖面形狀會根據液量而變化，故可藉由自上方之目視掌握液量。且，若於判斷為液量不足之情形時，必須補充液體。又，亦有為了去除因細胞等之活動而生成之代謝物，定期地進行液體之更換之情形。以下，對上述各孔之構造如何對此種操作起作用進行說明。In the holes W (W1 to W9) of the above-described respective configurations, a relatively simple shape such as a circular shape or a rectangular shape is used, and a protruding portion that protrudes outward or inward is provided in one of the contours of the cross section. Thereby, since the cross-sectional shape changes depending on the amount of liquid, the amount of liquid can be grasped by visual observation from above. Further, if it is determined that the amount of liquid is insufficient, the liquid must be replenished. Further, in order to remove metabolites generated by activities such as cells, it is also possible to periodically replace the liquid. Hereinafter, how the structure of each of the above holes acts on such an operation will be described.

對孔注入或更換培養液所必要之液量或作業內容可能於每個孔有差異。因此，操作者一面確認各孔內之液體之狀態一面藉由手動作業進行。具體而言，必須進行如下作業：將前端較細之移液管等注液用器具自孔之上部***孔內，於孔注入所需量之液體。此時，藉由手動作業難以將器具之前端相對於孔迅速且準確地進行定位。因此，即使熟練者亦需要作業時間，且有可能造成液體向孔外漏出，或注入量不準確。The amount of liquid or work necessary to inject or replace the culture fluid may vary from well to hole. Therefore, the operator performs the manual operation while confirming the state of the liquid in each hole. Specifically, it is necessary to perform an operation of inserting a desired amount of liquid into the hole by inserting a liquid infusion device such as a pipette having a narrow tip from the upper portion of the hole into the hole. At this time, it is difficult to quickly and accurately position the front end of the appliance with respect to the hole by manual work. Therefore, even a skilled person needs work time, and there is a possibility that liquid leaks out of the hole, or the amount of injection is inaccurate.

關於此，上述各構造之孔於側壁面具有用於液量掌握之突出部位。此種突出部位亦可作為操作者***具時之基準發揮功能。即，保持器具之操作者藉由將器具之前端抵接於突出部位，可容易地確定器具之前端相對於孔之位置。藉此，可迅速地進行準確之注液作業。又，即使於更換培養液時，藉由將用於自孔吸引培養液之移液管之前端抵接於任意之突出部位而吸引孔內之原有培養液，可使固定量之原有培養液殘留於孔內且迅速地將培養液去除直至特定之液面高度。藉此，即使並非熟練者亦可更換為準確量之培養液。In this regard, the holes of the above-described respective structures have protruding portions for grasping the amount of liquid on the side wall surface. Such a protruding portion can also function as a benchmark when the operator positions the instrument. That is, the operator of the holding device can easily determine the position of the front end of the appliance relative to the hole by abutting the front end of the appliance to the protruding portion. Thereby, an accurate liquid filling operation can be performed quickly. Further, even when the culture solution is changed, the original culture solution in the hole can be sucked by abutting the front end of the pipette for sucking the culture solution from the hole, thereby allowing a fixed amount of the original culture. The liquid remains in the well and the culture is quickly removed until a specific level is reached. Thereby, even if it is not a skilled person, it can be replaced with an accurate amount of the culture solution.

又，為了更有效地支援此種作業，突出部位亦可如以下般構成。再者，於以下針對幾個變形例僅圖示說明孔之一部分。然而，針對省略了圖示及說明之部分亦可應用同樣之想法進行變形。Moreover, in order to support such work more effectively, the protruding portion can also be configured as follows. Further, only one of the holes will be illustrated in the following for several modifications. However, the same idea can be applied to the parts in which the illustration and the description are omitted.

圖12及圖13係表示突出部位之形狀之變化例之圖。例如，於如圖2所示般突出部位自孔側壁面向內側突出之態樣之孔W1中，有自孔W1之上部注入之液體附著於突出部位114、115、116之頂面而將突出部位覆蓋住之情形。因此，於圖12(a)所示之變化例之孔W11中，突出部位114a之上表面114b成為於自側壁面112a朝向孔中央部之方向上具有向下之梯度之傾斜面。根據此種構成，由於液體不會停留於突出部位114a之上表面114b而向下方流下，故可避免液體殘留於上表面114b。上表面114b相對於鉛直方向所成之角θ較佳為30度至60度，例如可設為45度。對於以下之各變化例所記載之角θ亦相同。Fig. 12 and Fig. 13 are views showing a variation of the shape of the protruding portion. For example, in the hole W1 in which the protruding portion protrudes inward from the side wall of the hole as shown in FIG. 2, the liquid injected from the upper portion of the hole W1 adheres to the top surface of the protruding portion 114, 115, 116 and the protruding portion Cover the situation. Therefore, in the hole W11 of the modification shown in Fig. 12 (a), the upper surface 114b of the protruding portion 114a is an inclined surface having a downward gradient from the side wall surface 112a toward the central portion of the hole. According to this configuration, since the liquid does not stay on the upper surface 114b of the protruding portion 114a and flows downward, it is possible to prevent the liquid from remaining on the upper surface 114b. The angle θ formed by the upper surface 114b with respect to the vertical direction is preferably from 30 to 60 degrees, and may be, for example, 45 degrees. The angle θ described in each of the following modifications is also the same.

又，於圖12(b)所示之變化例之孔W12中，對圖12(a)之變化例施加進一步變形。即，以包含突出部位114a之上表面114b與側面114c連接之棱線114d之一部分之方式設置向外凹陷之凹部114e。凹部114e之形狀並不限定於圖示者而為任意。Further, in the hole W12 of the modification shown in Fig. 12 (b), further modification is applied to the modification of Fig. 12 (a). That is, the outwardly recessed recess 114e is provided in such a manner as to include a portion of the ridgeline 114d to which the upper surface 114b of the protruding portion 114a is joined to the side surface 114c. The shape of the concave portion 114e is not limited to those shown in the drawings.

於此種構成中，藉由使操作者保持之管狀之移液管前端P抵接於凹部114e，而約束移液管前端P向水平方向之移動。藉此，可容易且準確地定位移液管前端P。該構造如圖12(c)所示，亦可同樣應用於上表面水平地形成之孔W1之突出部位114。In such a configuration, the distal end P of the tubular pipette held by the operator abuts against the concave portion 114e, thereby restricting the movement of the pipette tip end P in the horizontal direction. Thereby, the pipette tip P can be easily and accurately positioned. This configuration is also applicable to the projecting portion 114 of the hole W1 formed horizontally on the upper surface as shown in Fig. 12(c).

圖13(a)、圖13(b)及圖13(c)分別表示對於圖3所示之孔W2、圖5所示之孔W4及圖8所示之孔W7之變化例。於圖13(a)所示之變化例之孔W13中，自孔底面向上延伸之柱狀之突出部位124a之上表面124b成為於自側壁面向孔中央部之方向上具有向下之梯度之傾斜面。該情形時亦可如以虛線所示般設置用於抵接移液管之凹部124e。13(a), 13(b) and 13(c) show examples of variations to the hole W2 shown in Fig. 3, the hole W4 shown in Fig. 5, and the hole W7 shown in Fig. 8. In the hole W13 of the modification shown in Fig. 13 (a), the upper surface 124b of the columnar projecting portion 124a extending upward from the bottom surface of the hole has a downward gradient from the side wall toward the central portion of the hole. surface. In this case, the recess 124e for abutting the pipette may be provided as indicated by a broken line.

於圖13(b)所示之變化例之孔W14中，階梯狀地設置之突出部位140a之上表面144b、145b、146b各者成為於自側壁面向孔中央部之方向上具有向下之梯度之傾斜面。於該情形時亦可設置用於抵接移液管之凹部144e。此處僅於一個階梯狀之上表面144b圖示有凹部144e，但亦可於其他上表面145b、146b亦設置凹部。In the hole W14 of the modification shown in FIG. 13(b), the upper surfaces 144b, 145b, and 146b of the protruding portion 140a which are provided in a stepped manner have a downward gradient in the direction from the side wall toward the center of the hole. Inclined surface. In this case, a recess 144e for abutting the pipette may also be provided. Here, the concave portion 144e is illustrated on only one stepped upper surface 144b, but the other upper surfaces 145b, 146b may also be provided with a concave portion.

於圖13(c)所示之變化例之孔W15中，突出部位170a上端之平面部174b成為於自側壁面向孔中央部之方向上具有向下之梯度之傾斜面。由於傾斜面部175b具有圓周方向上之梯度，故是否要設置自側壁面向孔中央部之方向、即徑向之梯度為任意。該情形時亦可設置用於抵接移液管之凹部174e。In the hole W15 of the modification shown in Fig. 13 (c), the flat portion 174b at the upper end of the protruding portion 170a is an inclined surface having a downward gradient in the direction from the side wall toward the central portion of the hole. Since the inclined surface portion 175b has a gradient in the circumferential direction, it is necessary to set the direction from the side wall toward the central portion of the hole, that is, the gradient in the radial direction. In this case, a recess 174e for abutting the pipette may also be provided.

另一方面，圖13(d)所示之變化例係對於圖4所示之孔W3之變化例。於該孔W3中，自孔開口面131向外側突出之突出部位134本身可作為抵接移液管時之位置基準而發揮功能。因此，於對此之變化例之孔W16中，為了防止注入之液體附著於突出部位134a之下表面134b，該下表面134b成為傾斜面。該情形時亦可進而設置用於抵接移液管之凹部134e。On the other hand, the variation shown in Fig. 13 (d) is a modification of the hole W3 shown in Fig. 4. In the hole W3, the protruding portion 134 which protrudes outward from the hole opening surface 131 itself functions as a positional reference when the pipette is abutted. Therefore, in the hole W16 of the modification, the lower surface 134b becomes an inclined surface in order to prevent the injected liquid from adhering to the lower surface 134b of the protruding portion 134a. In this case, a recess 134e for abutting the pipette can be further provided.

對於此處未記載變化例之各孔，亦基於與上述相同之想法，對於突出部位具有上表面者將其上表面設為傾斜面，又，對於突出部位具有下表面者將其下表面設為傾斜面。藉此，可不使注入之液體滯留而向下方流下。又，藉由根據需要設置凹部，可提高注液作業之作業性及作業精度。In the same manner as described above, the hole having the upper surface of the protruding portion has an upper surface which is an inclined surface, and the lower surface of the protruding portion has a lower surface. Inclined surface. Thereby, it is possible to flow down without causing the injected liquid to stay. Further, by providing the concave portion as needed, the workability and the work accuracy of the liquid filling operation can be improved.

尤其於進行培養液之更換之情形時，有***於孔之器具前端與培養之細胞等接觸而造成損傷之擔憂。然而，藉由上述構造容易進行器具前端之定位，藉此，此種問題亦可消除。In particular, when the culture solution is replaced, there is a concern that the tip of the device inserted into the hole comes into contact with the cultured cells or the like to cause damage. However, the positioning of the front end of the appliance is easily performed by the above configuration, whereby such a problem can be eliminated.

如以上所說明般，於上述實施形態中，孔板10作為本發明之「樣品容器」發揮功能。又，於上述各例中設置複數個突出部位之構成中，該等突出部位一體地作為本發明之「突出部」發揮功能。又，於具有單一之突出部位之構成中，該突出部位相當於本發明之「突出部」。As described above, in the above embodiment, the orifice plate 10 functions as the "sample container" of the present invention. Moreover, in the configuration in which a plurality of protruding portions are provided in each of the above examples, the protruding portions integrally function as "protrusions" of the present invention. Further, in the configuration having a single protruding portion, the protruding portion corresponds to the "protrusion portion" of the present invention.

又，於圖2及圖9所示之各例中，若將各圖(d)欄所示之距離液面S之開口面之深度設為「第1深度」，則此時之內部空間SP之剖面相當於本發明之「第1剖面」。且，距離如(e)欄及(f)欄所示之液面S之開口面之深度分別相當於相對於第1深度之「第2深度」，此時之內部空間SP之剖面相當於本發明之「第2剖面」。另一方面，若將距離各圖(e)欄所示之液面S之開口面之深度設為「第1深度」，則距離(f)欄所示之液面S之開口面之深度相當於相對於第1深度之「第2深度」。Further, in each of the examples shown in FIG. 2 and FIG. 9, when the depth of the opening surface from the liquid surface S shown in the column (d) is "first depth", the internal space SP at this time. The cross section corresponds to the "first section" of the present invention. Further, the depths of the opening faces of the liquid surface S as shown in the columns (e) and (f) correspond to the "second depth" with respect to the first depth, and the cross section of the internal space SP at this time corresponds to the present. The second section of the invention. On the other hand, if the depth of the opening surface of the liquid surface S shown in the column (e) of each figure is "first depth", the depth of the opening surface of the liquid surface S indicated by the distance (f) is equivalent. The "second depth" relative to the first depth.

再者，本發明並不限定於上述實施形態，可於不脫離其主旨之範圍內除上述者以外進行各種變更。例如，上述各例之孔具有大致圓形之水平剖面。然而，並不限定於此，例如對具有矩形剖面之孔亦可應用本發明。It is to be noted that the present invention is not limited to the above-described embodiments, and various modifications may be made without departing from the spirit and scope of the invention. For example, the holes of the above examples have a substantially circular horizontal cross section. However, it is not limited thereto, and the present invention can also be applied to, for example, a hole having a rectangular cross section.

又，於上述中示出了若干個突出部位之高度呈3階段不同之例，但高度之階段只要為2以上則為任意。Further, in the above description, the heights of the plurality of protruding portions are different in three stages, but the height is only required to be 2 or more.

又，於上述各例中孔之開口與水平面一致，但開口未必為水平。於該情形時，只要實現如下之孔之構造即可，即，將投影於適當之水平面之開口之形狀視作開口面之形狀，於更深之位置處之各剖面在俯視下包含於開口面之內部。Further, in the above examples, the opening of the hole coincides with the horizontal plane, but the opening is not necessarily horizontal. In this case, it is only necessary to realize a structure in which the shape of the opening projected in the appropriate horizontal plane is regarded as the shape of the opening surface, and the cross sections at the deeper position are included in the opening surface in plan view. internal.

又，上述之孔板10具有分別能夠儲存液體之複數個孔W。然而，本發明之「樣品容器」並不限定於此種形態者。例如，於僅具有1個儲存液體之儲存空間之被稱為「盤」或「培養皿」等之樣品容器中亦可應用本發明。Further, the above-mentioned orifice plate 10 has a plurality of holes W capable of storing liquids, respectively. However, the "sample container" of the present invention is not limited to such a form. For example, the present invention can also be applied to a sample container called a "disc" or a "dish dish" having only one storage space for storing liquid.

以上，如例示具體之實施形態進行之說明，該發明之樣品容器例如可構成為，設置有自孔之側壁面向內部空間之中央部側突出之突出部，突出部包含出現於第1剖面但未出現於第2剖面之部位、及出現於第1剖面及第2剖面兩者之部位。或者，可構成為，例如孔之側壁面之一部分成為朝自內部空間向外側之方向突出之突出部，突出部包含出現於第1剖面但未出現於第2剖面之部位、及出現於第1剖面及第2剖面兩者之部位。As described above, the sample container of the present invention is configured such that a protruding portion that protrudes from the side wall of the inner surface toward the central portion of the inner space is provided, and the protruding portion includes the first cross section but does not It appears at the portion of the second cross section and at the location of both the first cross section and the second cross section. Alternatively, for example, one of the side wall surfaces of the hole may be a protruding portion that protrudes outward from the inner space, and the protruding portion includes a portion that appears in the first cross section but does not appear in the second cross section, and appears in the first portion. The part of both the section and the second section.

根據此種構成，出現於液面之突出部之數量或形狀根據液量而變化。即，可根據於自上方觀察時出現於液面之突出部之數量或形狀而在視覺上表示孔內之液體量。According to this configuration, the number or shape of the projections appearing on the liquid surface varies depending on the amount of liquid. That is, the amount of liquid in the pores can be visually expressed in accordance with the number or shape of the projections appearing on the liquid surface when viewed from above.

於該等情形時，突出部可構成為具有鉛直方向之高度彼此不同且分別面向內部空間之複數個頂部。更具體而言，例如，突出部可構成為具有沿側壁面於鉛直方向延伸設置之複數個柱狀部位，各柱狀部位之上表面形成頂部。根據此種構成，成為如下狀態：自上方觀察時於較液面更上方具有頂面之柱狀部位看上去自液面露出，另一方面，於較液面更下方具有頂面之柱狀部位淹沒於液體中。因此，根據此種外觀之不同能夠判定液量。In such cases, the protrusions may be configured to have a plurality of tops that differ in height from each other in the vertical direction and that face the inner space, respectively. More specifically, for example, the protruding portion may be configured to have a plurality of columnar portions extending in the vertical direction along the side wall surface, and the upper surface of each of the columnar portions forms a top portion. According to such a configuration, the columnar portion having the top surface above the liquid surface when viewed from above appears to be exposed from the liquid surface, and the columnar portion having the top surface below the liquid surface. Submerged in liquid. Therefore, the amount of liquid can be determined based on the difference in appearance.

又，例如，突出部可設為以下構成中之任一者： (1)具有面之法線包含沿鉛直方向朝上之成分且鉛直方向之高度於水平方向連續變化之上表面的構成； (2)具有鉛直方向之高度彼此不同且分別面向內部空間之複數個底部之構成； (3)具有沿側壁面於鉛直方向延伸設置之複數個槽部位且各槽部位之上表面形成底部的構成； (4)具有面之法線包含沿鉛直方向朝上之成分且鉛直方向之高度於水平方向連續變化之下表面的構成。 於該等之構成之任一者，因液量之不同而引起液面高度之不同，故自上方觀察液面時之形狀因液量而有差異。因此，根據液面之形狀能夠容易視認液量。Further, for example, the protruding portion may be any of the following configurations: (1) a configuration in which the normal line of the surface includes a component facing upward in the vertical direction and the height in the vertical direction continuously changes the upper surface in the horizontal direction; 2) a structure having a plurality of bottom portions having different heights in the vertical direction and facing the inner space, respectively; (3) having a plurality of groove portions extending in the vertical direction along the side wall surface and forming a bottom portion on the upper surface of each groove portion; (4) A configuration in which the normal line of the surface includes a component that faces upward in the vertical direction and the height in the vertical direction continuously changes in the horizontal direction. In any of these configurations, the liquid level is different depending on the amount of liquid. Therefore, the shape of the liquid surface when viewed from above differs depending on the amount of liquid. Therefore, the amount of liquid can be easily recognized depending on the shape of the liquid surface.

於上述各構成中，進而例如可設為如下構成：鉛直方向上之一深度位置處之內部空間之水平剖面於俯視下包含較該深度更深之任意位置處之內部空間之水平剖面。根據此種構成，液量較少時液面之形狀為將液量更多而液面更高時之液面之形狀部分地切除而成者。然而，遮蔽該形狀之構造於較液面更上方不存在。因此，藉由自上方之觀察可準確地掌握液面之形狀。Further, in each of the above configurations, for example, a horizontal cross section of the internal space at one depth position in the vertical direction may include a horizontal cross section of the internal space at any position deeper than the depth in plan view. According to this configuration, when the amount of liquid is small, the shape of the liquid surface is partially cut off by the shape of the liquid surface when the amount of liquid is larger and the liquid level is higher. However, the configuration that obscures the shape does not exist above the liquid level. Therefore, the shape of the liquid surface can be accurately grasped by observation from above.

又，例如，側壁面可構成為，於鉛直方向之特定高度之位置，形成相對於較該高度更上方之位置於該高度更下方之位置內部空間之水平截面面積減少之階差部，第2剖面為較階差部更上方之水平剖面。根據本案發明者之見解，於具有此種階差部之孔中，可抑制於拍攝孔內時因液面之彎月面孔導致底面周緣部變暗之現象。該效果可藉由使液面位於階差部更上方而獲得。因此，只要可於液面下降至階差部更下方之前檢測到該情況，則可確實地獲得上述效果。Further, for example, the side wall surface may be formed at a position at a specific height in the vertical direction, and a step portion in which the horizontal cross-sectional area of the internal space is reduced at a position higher than the height from the height is formed, and the second step is formed. The profile is a horizontal section above the step. According to the findings of the inventors of the present invention, in the hole having such a step portion, it is possible to suppress the phenomenon that the peripheral portion of the bottom surface is darkened by the meniscus of the liquid surface when the hole is photographed. This effect can be obtained by setting the liquid level above the step portion. Therefore, as long as the situation can be detected before the liquid level falls below the step portion, the above effect can be surely obtained.

又，例如，可設為第2剖面對應於儲存於內部空間之液體之液面高度之下限之構成。根據此種構成，藉由液面之形狀變化可判斷液量大於下限值或小於下限值。藉此，例如可根據需要採取補充液體等措施。Further, for example, it is possible to adopt a configuration in which the second cross section corresponds to the lower limit of the liquid level of the liquid stored in the internal space. According to this configuration, it is possible to determine that the liquid amount is larger than the lower limit value or smaller than the lower limit value by the change in the shape of the liquid surface. Thereby, for example, measures such as replenishing the liquid can be taken as needed.

又，例如，突出部若為具有上表面者則其上表面可為自側壁面向內部空間之中央側向下傾斜之傾斜面，突出部若為具有下表面者則其下表面可為自側壁面向內部空間之中央側向下傾斜之傾斜面。又，例如於突出部之上表面與側面之連接部分中亦可具有一部分向外凹陷之凹部。根據該等構成，可提高操作者使用注液用之器具將液體注入孔時之作業性及作業精度。 [產業上之可利用性]Further, for example, if the protruding portion has an upper surface, the upper surface thereof may be an inclined surface that is inclined downward from a central side of the side wall facing the inner space, and if the protruding portion has a lower surface, the lower surface thereof may be a self-side facing surface. An inclined surface that is inclined downward toward the center side of the internal space. Further, for example, the connecting portion between the upper surface and the side surface of the protruding portion may have a recessed portion which is partially recessed outward. According to these configurations, the workability and work accuracy when the operator injects the liquid into the hole using the tool for injecting liquid can be improved. [Industrial availability]

本發明可應用於所有將細胞等培養對象物與液體一起保持之樣品容器。尤其，適合於將藉由培養製作出之樣品以供拍攝之目的。此種樣品容器可較佳應用於醫學、生化學、藥物開發等領域。The present invention can be applied to all sample containers in which a culture object such as a cell is held together with a liquid. In particular, it is suitable for the purpose of photographing a sample produced by culturing. Such a sample container can be preferably used in the fields of medicine, biochemistry, drug development and the like.

10‧‧‧孔板(樣品容器)10‧‧‧ orifice plate (sample container)

10a‧‧‧上表面10a‧‧‧ upper surface

11‧‧‧基材11‧‧‧Substrate

111‧‧‧(孔W1之)開口面111‧‧‧ (opening of hole W1)

112‧‧‧(孔W1之)側壁面112‧‧‧ (hole W1) side wall surface

112a‧‧‧側壁面112a‧‧‧ Side wall

113‧‧‧(孔W1之)底面113‧‧‧ (hole W1) bottom surface

114‧‧‧(孔W1之)突出部位114‧‧‧ (hole W1) protruding parts

114a‧‧‧突出部位114a‧‧‧ prominent parts

114b‧‧‧上表面114b‧‧‧ upper surface

114c‧‧‧側面114c‧‧‧ side

114d‧‧‧棱線114d‧‧‧ ridgeline

114e‧‧‧凹部114e‧‧‧ recess

115‧‧‧(孔W1之)突出部位115‧‧‧ (hole W1) protruding parts

116‧‧‧(孔W1之)突出部位116‧‧‧ (hole W1) protruding parts

121‧‧‧開口面121‧‧‧Open face

122‧‧‧側壁面122‧‧‧ side wall

123‧‧‧底面123‧‧‧ bottom

124、125、126‧‧‧(孔W2之)柱狀部位124, 125, 126‧‧‧ (hole W2) columnar part

124a‧‧‧突出部位124a‧‧‧ prominent parts

124b‧‧‧上表面124b‧‧‧ upper surface

124e‧‧‧凹部124e‧‧‧ recess

131‧‧‧開口面131‧‧‧Open face

132‧‧‧側壁面132‧‧‧ side wall

133‧‧‧底面133‧‧‧ bottom

134‧‧‧(孔W3之)槽部位134‧‧‧ (hole W3) groove

134a‧‧‧突出部位134a‧‧‧ prominent parts

134b‧‧‧下表面134b‧‧‧ lower surface

134e‧‧‧凹部134e‧‧‧ recess

135‧‧‧(孔W3之)槽部位135‧‧‧ (hole W3) groove

136‧‧‧(孔W3之)槽部位136‧‧‧ (hole W3) groove

140‧‧‧突出部位140‧‧‧ prominent parts

140a‧‧‧突出部位140a‧‧‧ prominent parts

141‧‧‧開口面141‧‧‧Open face

142‧‧‧側壁面142‧‧‧ side wall

144‧‧‧平面部144‧‧‧Flat Department

144b‧‧‧上表面144b‧‧‧ upper surface

144e‧‧‧凹部144e‧‧‧ recess

145‧‧‧平面部145‧‧‧Flat Department

145b‧‧‧上表面145b‧‧‧ upper surface

146‧‧‧平面部146‧‧‧Flat Department

146b‧‧‧上表面146b‧‧‧ upper surface

150‧‧‧突出部位150‧‧‧ prominent parts

151‧‧‧開口面151‧‧‧Open face

152‧‧‧側壁面152‧‧‧ side wall

154‧‧‧平面部154‧‧‧Flat Department

155‧‧‧平面部155‧‧‧Flat Department

156‧‧‧平面部156‧‧‧Flat Department

160‧‧‧突出部位160‧‧‧ prominent parts

161‧‧‧開口面161‧‧‧Open face

162‧‧‧側壁面162‧‧‧ side wall

164‧‧‧平面部164‧‧‧Flat Department

165‧‧‧平面部165‧‧‧Flat Department

166‧‧‧底面部166‧‧‧ bottom part

170‧‧‧突出部位170‧‧‧ prominent parts

170a‧‧‧突出部位170a‧‧‧ prominent parts

171‧‧‧開口面171‧‧‧Open face

172‧‧‧側壁面172‧‧‧ side wall

174‧‧‧平面部174‧‧‧Flat Department

174b‧‧‧平面部174b‧‧‧Flat Department

174e‧‧‧凹部174e‧‧‧ recess

175‧‧‧傾斜面部175‧‧‧Sloping face

175b‧‧‧傾斜面部175b‧‧‧Sloping face

176‧‧‧後端部176‧‧‧ back end

180‧‧‧突出部位180‧‧‧ protruding parts

181‧‧‧開口面181‧‧‧Open face

182‧‧‧側壁面182‧‧‧ side wall

184‧‧‧上端部184‧‧‧ upper end

185‧‧‧傾斜面部185‧‧‧Sloping face

186‧‧‧下端部186‧‧‧ lower end

191‧‧‧開口面191‧‧‧Open face

192‧‧‧側壁面192‧‧‧ side wall

193‧‧‧底面193‧‧‧ bottom

194、195、196‧‧‧突出部位194, 195, 196‧‧ ‧ prominent parts

197‧‧‧階差部197‧‧ ‧ step department

198‧‧‧側壁面198‧‧‧ side wall

A-A‧‧‧線A-A‧‧‧ line

CM‧‧‧攝像機構CM‧‧· camera organization

L‧‧‧光L‧‧‧Light

LS‧‧‧光源LS‧‧‧ light source

Mk‧‧‧標記Mk‧‧ tag

P‧‧‧移液管前端P‧‧‧ pipette front end

S‧‧‧液面S‧‧‧ liquid level

SP‧‧‧內部空間SP‧‧‧Internal space

SP1‧‧‧第1空間SP1‧‧‧1st space

SP2‧‧‧第2空間SP2‧‧‧Second space

W‧‧‧孔W‧‧‧ hole

W1‧‧‧孔W1‧‧ hole

W2‧‧‧孔W2‧‧‧ hole

W3‧‧‧孔W3‧‧‧ hole

W4‧‧‧孔W4‧‧‧ hole

W5‧‧‧孔W5‧‧‧ hole

W6‧‧‧孔W6‧‧‧ hole

W7‧‧‧孔W7‧‧‧ hole

W8‧‧‧孔W8‧‧‧ hole

W9‧‧‧孔W9‧‧‧ hole

W11‧‧‧孔W11‧‧‧ hole

W12‧‧‧孔W12‧‧ hole

W13‧‧‧孔W13‧‧ hole

W14‧‧‧孔W14‧‧ hole

W15‧‧‧孔W15‧‧ hole

W16‧‧‧孔W16‧‧ hole

X‧‧‧方向X‧‧‧ direction

Y‧‧‧方向Y‧‧‧ direction

Z‧‧‧方向Z‧‧‧ direction

θ‧‧‧角Θ‧‧‧ corner

圖1A及圖1B係表示作為樣品容器之一例之孔板之整體構造之圖。 圖2(a)～(f)係表示具有本發明之內部構造之孔之第1例之圖。 圖3(a)～(f)係表示具有本發明之內部構造之孔之第2例之圖。 圖4(a)～(f)係表示具有本發明之內部構造之孔之第3例之圖。 圖5(a)～(f)係表示具有本發明之內部構造之孔之第4例之圖。 圖6(a)～(f)係表示具有本發明之內部構造之孔之第5例之圖。 圖7(a)～(f)係表示具有本發明之內部構造之孔之第6例之圖。 圖8(a)～(f)係表示具有本發明之內部構造之孔之第7例之圖。 圖9(a)～(f)係表示具有本發明之內部構造之孔之第8例之圖。 圖10A至圖10C係表示具有本發明之內部構造之孔之第9例之圖。 圖11係例示承載於具有階差之孔之液體之攝像之圖。 圖12A至圖12C係表示突出部位之形狀之變化例之圖。 圖13A至圖13D係表示突出部位之形狀之另一變化例之圖。1A and 1B are views showing the entire structure of an orifice plate as an example of a sample container. 2(a) to 2(f) are views showing a first example of a hole having an internal structure of the present invention. 3(a) to 3(f) are views showing a second example of a hole having the internal structure of the present invention. 4(a) to 4(f) are views showing a third example of a hole having the internal structure of the present invention. 5(a) to 5(f) are views showing a fourth example of a hole having the internal structure of the present invention. 6(a) to 6(f) are views showing a fifth example of a hole having the internal structure of the present invention. 7(a) to 7(f) are views showing a sixth example of a hole having the internal structure of the present invention. 8(a) to 8(f) are views showing a seventh example of a hole having the internal structure of the present invention. 9(a) to 9(f) are views showing an eighth example of a hole having the internal structure of the present invention. 10A to 10C are views showing a ninth example of a hole having the internal structure of the present invention. Fig. 11 is a view showing an image of a liquid carried on a hole having a step. 12A to 12C are views showing a variation of the shape of the protruding portion. 13A to 13D are views showing another variation of the shape of the protruding portion.

Claims (20)

一種樣品容器，其具有能夠於上部開口之內部空間儲存液體，且設有顯示上述液體之上限值及下限值之各者之突出部之孔；且於水平姿勢之上述樣品容器中，將投影於水平面之上述內部空間之開口設為開口面，將自上述開口鉛直向下特定之第1深度之位置處之上述內部空間之水平剖面設為第1剖面，將較上述第1深度更深之第2深度位置處之上述內部空間之水平剖面設為第2剖面時，上述開口面、上述第1剖面及上述第2剖面之外周形狀彼此不相似，於俯視下，上述第2剖面包含於第1剖面內，上述第1剖面包含於上述開口面內。 A sample container having a hole capable of storing a liquid in an inner space of an upper opening and provided with a protruding portion for displaying each of an upper limit value and a lower limit value of the liquid; and in the sample container of the horizontal posture, An opening of the internal space projected on the horizontal plane is an opening surface, and a horizontal cross section of the internal space at a position at which the opening is vertically downward is defined as a first cross section, and is deeper than the first depth When the horizontal cross section of the internal space at the second depth position is the second cross section, the outer peripheral shapes of the opening surface, the first cross section, and the second cross section are not similar to each other, and the second cross section is included in the plan view. In the first section, the first cross section is included in the opening surface. 如請求項1之樣品容器，其設置自上述孔之側壁面朝向上述內部空間之中央部側突出之上述突出部，上述突出部包含出現於上述第1剖面而未出現於上述第2剖面之部位、及出現於上述第1剖面及上述第2剖面兩者之部位。 The sample container according to claim 1, wherein the protruding portion protrudes from a side wall surface of the hole toward a central portion side of the inner space, and the protruding portion includes a portion that appears in the first cross section and does not appear in the second cross section. And appearing in both the first cross section and the second cross section. 如請求項1之樣品容器，其中上述孔之側壁面之一部分成為朝自上述內部空間向外側之方向突出之上述突出部，上述突出部包含出現於上述第1剖面而未出現於上述第2剖面之部位、及出現於上述第1剖面及上述第2剖面兩者之部位。 The sample container according to claim 1, wherein one of the side wall surfaces of the hole protrudes toward the outer side from the inner space, and the protruding portion includes the first cross section and does not appear in the second cross section. The portion and the portion appearing in both the first cross section and the second cross section. 一種樣品容器，其具有能夠於上部開口之內部空間儲存液體之孔； 且設置有自上述孔之底部沿上述孔之側壁面朝上突出，且顯示上述液體之上限值及下限值之各者之突出部；於水平姿勢之上述樣品容器中，將自上述內部空間之開口鉛直向下特定之第1深度之位置處之上述內部空間之水平剖面設為第1剖面，將較上述第1深度更深之第2深度位置處之上述內部空間之水平剖面設為第2剖面時，於俯視下，上述第2剖面包含於上述第1剖面內，上述突出部包含出現於上述第1剖面而未出現於上述第2剖面之部位、及出現於上述第1剖面及上述第2剖面兩者之部位。 a sample container having a hole capable of storing a liquid in an inner space of an upper opening; And a protrusion protruding from a bottom surface of the hole along a side wall surface of the hole and displaying each of an upper limit value and a lower limit value of the liquid; and the sample container in a horizontal posture is from the inside The horizontal cross section of the internal space at the position where the opening of the space is vertically downward is defined as the first cross section, and the horizontal cross section of the internal space at the second depth position deeper than the first depth is In the case of the second cross section, the second cross section is included in the first cross section, and the protruding portion includes a portion that appears in the first cross section and does not appear in the second cross section, and appears in the first cross section and the The part of both the second section. 如請求項2或4之樣品容器，其中上述突出部具有鉛直方向之高度彼此不同且各自面向上述內部空間之複數個頂部。 The sample container of claim 2 or 4, wherein the protrusions have a height in a vertical direction different from each other and each facing a plurality of tops of the inner space. 如請求項5之樣品容器，其中上述突出部具有沿上述側壁面於鉛直方向延伸設置之複數個柱狀部位，各個上述柱狀部位之上表面形成上述頂部。 The sample container according to claim 5, wherein the protruding portion has a plurality of columnar portions extending in a vertical direction along the side wall surface, and the top surface of each of the columnar portions forms the top portion. 如請求項2或4之樣品容器，其中上述突出部具有面之法線具有沿鉛直方向朝上之成分且鉛直方向之高度於水平方向連續變化之上表面。 The sample container of claim 2 or 4, wherein the protrusion has a surface normal having a component facing upward in the vertical direction and a height in the vertical direction continuously changing the upper surface in the horizontal direction. 如請求項5之樣品容器，其中上述突出部之上表面為自上述側壁面朝向上述內部空間之中央側向下傾斜之傾斜面。 The sample container according to claim 5, wherein the upper surface of the protruding portion is an inclined surface which is inclined downward from the side wall surface toward the center side of the inner space. 如請求項5之樣品容器，其中上述突出部具有於上述突出部之上表面與側面之連接部分中一部分向外凹陷之凹部。 The sample container of claim 5, wherein the protrusion has a recess that is partially recessed in a portion of the connecting portion between the upper surface and the side surface of the protruding portion. 如請求項7之樣品容器，其中上述突出部之上表面為自上述側壁面朝向上述內部空間之中央側向下傾斜之傾斜面。 The sample container according to claim 7, wherein the upper surface of the protruding portion is an inclined surface which is inclined downward from the side wall surface toward the center side of the inner space. 如請求項7之樣品容器，其中上述突出部具有於上述突出部之上表面與側面之連接部分中一部分向外凹陷之凹部。 The sample container of claim 7, wherein the protrusion has a recess in which a portion of the connecting portion of the upper surface and the side surface of the protruding portion is outwardly recessed. 如請求項3之樣品容器，其中上述突出部具有鉛直方向之高度彼此不同且各自面向上述內部空間之複數個頂部。 The sample container of claim 3, wherein the protrusions have a height in a vertical direction different from each other and each facing a plurality of tops of the inner space. 如請求項12之樣品容器，其中上述突出部具有沿上述側壁面於鉛直方向延伸設置之複數個槽部位，各個上述槽部位之上表面形成上述底部。 The sample container according to claim 12, wherein the protruding portion has a plurality of groove portions extending in a vertical direction along the side wall surface, and the upper surface of each of the groove portions forms the bottom portion. 如請求項3之樣品容器，其中上述突出部具有面之法線具有沿鉛直方向朝上之成分且鉛直方向之高度於水平方向連續變化之下表面。 The sample container of claim 3, wherein the protrusion has a surface having a surface having a surface facing upward in a vertical direction and a height in a vertical direction continuously changing the surface below the horizontal direction. 如請求項12至14中任一項之樣品容器，其中上述突出部之下表面為自上述側壁面朝向上述內部空間之中央側向下傾斜之傾斜面。 The sample container according to any one of claims 12 to 14, wherein the lower surface of the protruding portion is an inclined surface which is inclined downward from the side wall surface toward the center side of the inner space. 如請求項12至14中任一項之樣品容器，其中上述突出部具有於上述 突出部之下表面與不同於上述突出部之側面之上述側壁面之連接部分中一部分向外凹陷之凹部。 The sample container of any one of claims 12 to 14, wherein the protrusion has the above a recessed portion of the connecting portion of the lower surface of the protruding portion and the side wall surface of the side surface different from the side surface of the protruding portion. 如請求項1至4及12至14中任一項之樣品容器，其中鉛直方向上之一深度位置處之上述內部空間之水平剖面於俯視下包含較該深度更深之任意位置處之上述內部空間之水平剖面。 The sample container according to any one of claims 1 to 4, wherein the horizontal section of the inner space at a depth position in the vertical direction includes the inner space at any position deeper than the depth in plan view Horizontal profile. 如請求項1至4及12至14中任一項之樣品容器，其中上述側壁面於鉛直方向之特定高度之位置，形成上述內部空間之水平截面面積在相對於較該高度為上之位置而為較該高度為下之位置減少之階差部，上述第2剖面為較上述階差部更上方之水平剖面。 The sample container according to any one of claims 1 to 4, wherein the side wall surface is at a specific height in a vertical direction, and the horizontal cross-sectional area of the inner space is formed at a position higher than the height. The stepped portion that is lower than the position where the height is lower is the horizontal cross section that is higher than the step portion. 如請求項1至4及12至14中任一項之樣品容器，其中上述第2剖面對應於儲存於上述內部空間之上述液體之液面高度之下限。 The sample container according to any one of claims 1 to 4, wherein the second cross section corresponds to a lower limit of a liquid level of the liquid stored in the inner space. 如請求項1至4及12至14中任一項之樣品容器，其中更具備突出部，其與成為儲存於上述內部空間之上述液體之補充標準之液面高度對應。The sample container according to any one of claims 1 to 4 and 12 to 14, further comprising a protruding portion corresponding to a liquid level which is a supplementary standard of the liquid stored in the internal space.
TW107117669A 2017-07-05 2018-05-24 Specimen container TWI667343B (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
JP2017-131651 2017-07-05
JP2017131651 2017-07-05
JP2017-183180 2017-09-25
JP2017183180 2017-09-25
JP2018-086223 2018-04-27
JP2018086223A JP2019050798A (en) 2017-07-05 2018-04-27 Sample container

Publications (2)

Publication Number Publication Date
TW201907000A TW201907000A (en) 2019-02-16
TWI667343B true TWI667343B (en) 2019-08-01

Family

ID=66012459

Family Applications (2)

Application Number Title Priority Date Filing Date
TW108120901A TWI689583B (en) 2017-07-05 2018-05-24 Specimen container
TW107117669A TWI667343B (en) 2017-07-05 2018-05-24 Specimen container

Family Applications Before (1)

Application Number Title Priority Date Filing Date
TW108120901A TWI689583B (en) 2017-07-05 2018-05-24 Specimen container

Country Status (2)

Country Link
JP (1) JP2019050798A (en)
TW (2) TWI689583B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110529355B (en) * 2019-09-09 2021-05-28 英华达（上海）科技有限公司 Liquid amount control device and liquid amount control method using same

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100028935A1 (en) * 2006-11-23 2010-02-04 Carlo Ciaiolo Convex bottom microwell
JP2010110262A (en) * 2008-11-06 2010-05-20 Hitachi Maxell Ltd Method for amplifying nucleic acid by using well plate
US20150313704A1 (en) * 2012-12-07 2015-11-05 The Governing Council Of The University Of Toronto Cardiac tissue constructs and methods of fabrication thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4724854B2 (en) * 2009-02-09 2011-07-13 大日本印刷株式会社 Cell culture vessel
TW201209158A (en) * 2010-06-17 2012-03-01 Geneasys Pty Ltd LOC device for genetic analysis with dialysis, chemical lysis and tandem nucleic acid amplification
US9790465B2 (en) * 2013-04-30 2017-10-17 Corning Incorporated Spheroid cell culture well article and methods thereof
JP2015195762A (en) * 2014-04-01 2015-11-09 大日本印刷株式会社 cell culture vessel
GB2539935A (en) * 2015-07-01 2017-01-04 Insphero Ag Device for propagating microtissues

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100028935A1 (en) * 2006-11-23 2010-02-04 Carlo Ciaiolo Convex bottom microwell
JP2010110262A (en) * 2008-11-06 2010-05-20 Hitachi Maxell Ltd Method for amplifying nucleic acid by using well plate
US20150313704A1 (en) * 2012-12-07 2015-11-05 The Governing Council Of The University Of Toronto Cardiac tissue constructs and methods of fabrication thereof

Also Published As

Publication number Publication date
JP2019050798A (en) 2019-04-04
TW201907000A (en) 2019-02-16
TWI689583B (en) 2020-04-01
TW201936917A (en) 2019-09-16

Similar Documents

Publication Publication Date Title
JP7214729B2 (en) Sample container for optical measurement
KR101140751B1 (en) Feces collection container
US9588104B2 (en) Device, a system and a method for monitoring and/or culturing of microscopic objects
JP5501141B2 (en) Preparation for liquid sample inspection
EP2270573A1 (en) Cover for a counting, viability assessment, analysis and manipulation chamber
JP5478456B2 (en) Preparation for liquid sample inspection
KR102230694B1 (en) Culture container
US11602751B2 (en) Liquid evaluation
TWI667343B (en) Specimen container
JP2014002046A (en) Preparation for inspection of liquid sample
ES2899683T3 (en) Device and method for controlling the volume of a sample
WO2016154361A1 (en) A method and related systems for use with a fluidics device
CN109207362B (en) Sample container
JP5376428B2 (en) Analytical device
EP2863733B1 (en) Sample collection and transfer assembly and related methods
US11534764B2 (en) Cartridge
JP7164174B2 (en) Tray for accommodating medical examination cassettes and method for printing examination information on medical examination cassettes using the same
JP2018063187A (en) Container structure and imaging system using the same
JP2008154579A (en) One action type cell kinetic-observing apparatus
EP2982989B1 (en) Receptacle containing test reagent
JP6412435B2 (en) Microchannel chip
CN110621770B (en) Container for PCR, container for PCR containing reagent, and kit
US20240139744A1 (en) Slide preparation methods and apparatus
JP2020043769A (en) Multiwell plate for model organisms