TWI638044B - Bioreactor system - Google Patents

Bioreactor system Download PDF

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TWI638044B
TWI638044B TW105124406A TW105124406A TWI638044B TW I638044 B TWI638044 B TW I638044B TW 105124406 A TW105124406 A TW 105124406A TW 105124406 A TW105124406 A TW 105124406A TW I638044 B TWI638044 B TW I638044B
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column
reaction system
biological reaction
accommodating space
disposed
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TW105124406A
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TW201805417A (en
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張嘉修
陳俊延
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國立成功大學
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Abstract

本揭露係關於一種生物反應系統,包括:一管柱,包括:一管柱頂端、一管柱底端及一管柱側部,其中管柱側部設於管柱頂端及管柱底端間且與管柱頂端及管柱底端相連;一底部,設於管柱之管柱底端,管柱與底部形成一容置空間,且底部具有一類弧型內底表面;一岐管,設於管柱外且具有一輸出口及複數輸入口,輸出口與底部連接,而輸入口與管柱側部連接;一曝氣裝置,與管柱頂端連接以提供一氣體至容置空間。 The present disclosure relates to a biological reaction system comprising: a pipe string comprising: a pipe top end, a pipe column bottom end and a pipe column side portion, wherein the pipe column side portion is disposed between the pipe column top end and the pipe column bottom end And connected to the top of the column and the bottom end of the column; a bottom is arranged at the bottom end of the column of the column, the tube column and the bottom form an accommodation space, and the bottom has a type of arc-shaped inner bottom surface; Outside the column and having an output port and a plurality of input ports, the output port is connected to the bottom portion, and the input port is connected to the side of the column column; an aeration device is connected to the top end of the column column to provide a gas to the accommodating space.

Description

生物反應系統 Biological reaction system

本揭露係關於一種生物反應系統,尤指一種氣舉式生物反應系統。 The disclosure relates to a biological reaction system, and more particularly to a gas lift biological reaction system.

微藻工業每年提供超過五千公噸的產量與12.5億美元的產值。此外,全球健康食品市場每年所使用的微藻量約為5,000噸,單價達每公斤330美元。近年來,更把微藻的應用面推廣到飲料、乳酪、優格等大宗民生消費物品市場,甚至添加到動物飼料中;而此應用面的年產能也超過240公噸,產值超過2億美元。 The microalgae industry provides more than 5,000 metric tons of production per year and an output value of $1.25 billion. In addition, the global health food market uses about 5,000 tons of microalgae per year at a unit price of $330 per kilogram. In recent years, the application of microalgae has been extended to the market of large-scale consumer goods such as beverages, cheeses, and yogurt, and even added to animal feed; the annual production capacity of this application has exceeded 240 metric tons, and the output value has exceeded 200 million US dollars.

除此之外,藻體中的螢光蛋白亦可使用在螢光性的標誌物上,例如與抗體、蛋白質A、維生素H、卵白素或凝集素做共價結合;且已知已經被開發成醫療用的檢驗試劑,價格介於每毫克2-25美元之間。 In addition, fluorescent proteins in algae can also be used on fluorescent markers, such as covalently bound to antibodies, protein A, vitamin H, avidin or lectin; and are known to have been developed A medical test reagent, the price is between 2-25 US dollars per milligram.

目前微藻養殖密閉式系統中,包括管狀光生物反應器、平板式光生物反應器與塑膠袋式光生物反應器。 At present, the microalgae culture closed system includes a tubular photobioreactor, a flat photobioreactor and a plastic bag photobioreactor.

然而,不論哪種類型光生物反應器,皆以在反應器底部裝設曝氣裝置,利用曝氣帶動微藻養殖系統中的循環。此外,不論哪種類型光生物反應器,底部均為矩形設計而多有死角,被培養物容易沉積於死角中,造成產率降低。 However, regardless of the type of photobioreactor, an aeration device is installed at the bottom of the reactor to aerate the circulation in the microalgae culture system. In addition, regardless of the type of photobioreactor, the bottom is rectangular in design and has many dead ends, and the culture is easily deposited in the dead corner, resulting in a decrease in yield.

因此,目前仍須發展一種能提升藻體生長效能及產量的裝置,以因應目前龐大的微藻市場商機。 Therefore, it is still necessary to develop a device that can enhance the growth efficiency and yield of algae in response to the current huge market of microalgae.

本揭露之主要目的係在提供一種生物反應系統,其可提升培養液於裝置中的循環效率,進而提升被培養物的生長效率。 The main object of the present disclosure is to provide a biological reaction system which can increase the circulation efficiency of the culture solution in the device, thereby improving the growth efficiency of the cultured object.

本揭露之一種生物反應系統,包括:一管柱,包括:一管柱頂端、一管柱底端及一管柱側部,其中管柱側部設於管柱頂端及管柱底端間且與管柱頂端及管柱底端相連;一底部,設於管柱之管柱底端,管柱與底部形成一容置空間,且底部具有一類弧型內底表面;一岐管,設於管柱外且具有一輸出口及複數輸入口,輸出口與底部連接,而輸入口與管柱側部連接;一曝氣裝置,與管柱頂端連接以提供一氣體至容置空間。 A biological reaction system according to the present disclosure includes: a pipe string comprising: a pipe top end, a pipe column bottom end and a pipe column side portion, wherein the pipe column side portion is disposed between the pipe column top end and the pipe column bottom end and Connected to the top of the column and the bottom end of the column; a bottom is disposed at the bottom end of the column of the column, the tube column and the bottom form an accommodation space, and the bottom has a curved inner bottom surface; Outside the column, there is an output port and a plurality of input ports, the output port is connected to the bottom, and the input port is connected to the side of the column; an aeration device is connected to the top end of the column to provide a gas to the accommodating space.

於本揭露之生物反應系統中,藉由設於管柱外的岐管及與管柱相連的曝氣裝置,而可達到氣舉式循環,進而提升被培養物的生長效率。特別是,於本揭露之生物反應系統中,設於管柱底端的底部具有一類弧型內底表面。相較於習知底部具有矩形內底表面的生物反應系統,於本揭露之生物反應系統中,當一培養液設置於由管住及底部所形成的容置空間中時,培養液於容置空間中的循環沒有死角,而可提升被培養物的生長效率。 In the biological reaction system disclosed in the present disclosure, the gas lift cycle can be achieved by the manifold disposed outside the column and the aeration device connected to the column, thereby improving the growth efficiency of the culture. In particular, in the biological reaction system of the present disclosure, the bottom portion provided at the bottom end of the tubular string has a curved inner bottom surface. Compared with the conventional biological reaction system having a rectangular inner bottom surface, in the biological reaction system of the present disclosure, when a culture liquid is disposed in the accommodating space formed by the tube and the bottom, the culture liquid is accommodated. The cycle in the space has no dead angle, but can improve the growth efficiency of the culture.

於本揭露之生物反應系統中,被培養物的種類並無特殊限制,較佳為藻類,如微藻。 In the biological reaction system of the present disclosure, the type of the culture to be cultured is not particularly limited, and is preferably algae such as microalgae.

於本揭露之生物反應系統中,管柱較佳為一圓柱形空心管柱。 In the biological reaction system of the present disclosure, the column is preferably a cylindrical hollow column.

於本揭露之生物反應系統中,管柱及底部可以本技術領域已知的任何方式連接。於本揭露之一實施態樣中,管柱及底部係透過一樞接件連接。於本揭露之另一實施態樣中,管柱及底部係一體成形。 In the biological reaction system of the present disclosure, the column and the bottom can be joined in any manner known in the art. In one embodiment of the present disclosure, the column and the bottom are connected by a pivotal member. In another embodiment of the present disclosure, the column and the bottom are integrally formed.

本揭露之生物反應系統更可包括一光源,設於管柱外或容置空間中。較佳為,光源設於容置空間中。 The biological reaction system of the present disclosure may further comprise a light source disposed outside the column or in the accommodating space. Preferably, the light source is disposed in the accommodating space.

於本揭露之生物反應系統中,底部可具有一類弧型內底表面。較佳為,底部具有一弧型內底表面。更佳為,弧型內表面係為一半圓形內底表面。 In the biological reaction system of the present disclosure, the bottom portion may have a curved inner bottom surface. Preferably, the bottom portion has an arcuate inner bottom surface. More preferably, the curved inner surface is a semi-circular inner bottom surface.

於本揭露之生物反應系統中,底部於管柱之一長度方向上具有一高度,底部於垂直管柱之長度方向上具有一寬度,而高度與寬度比係介於0.5至4.0之間。當高度與寬度比介於此範圍內時,可達到最佳的循環效果。 In the biological reaction system of the present disclosure, the bottom has a height in the longitudinal direction of one of the columns, the bottom has a width in the longitudinal direction of the vertical tubular string, and the height to width ratio is between 0.5 and 4.0. When the height to width ratio is within this range, the best cycle effect is achieved.

此外,於本揭露之生物反應系統中,一培養液係設於容置空間中;且當曝氣裝置提供氣體至容置空間中時,培養液係由輸出口流出並通過岐管而由輸入口循環至容置空間中。 In addition, in the biological reaction system of the present disclosure, a culture liquid is disposed in the accommodating space; and when the aeration device supplies the gas into the accommodating space, the cultivating liquid flows out from the output port and is input through the manifold The loop is looped into the accommodating space.

再者,於本揭露之生物反應系統中,輸出口與輸入口於管柱之一長度方向上的距離並無特殊限制,隨著距離的增加,則需設置更多的輸入口,以提升培養液的循環效果。較佳為,輸出口與輸入口於管柱之一長度方向上的距離係介於50cm至300cm之間。 Furthermore, in the biological reaction system disclosed in the present disclosure, the distance between the output port and the input port in the longitudinal direction of the column is not particularly limited, and as the distance increases, more input ports are required to enhance the culture. The circulation effect of the liquid. Preferably, the distance between the output port and the input port in the length direction of one of the columns is between 50 cm and 300 cm.

11‧‧‧管柱 11‧‧‧ column

111‧‧‧管柱頂端 111‧‧‧Top of the column

112‧‧‧管柱底端 112‧‧‧ bottom of the column

113‧‧‧管柱側部 113‧‧‧The side of the column

114‧‧‧上蓋 114‧‧‧Upper cover

12,115‧‧‧底部 12, 115‧‧‧ bottom

121‧‧‧類弧型內底表面 121‧‧‧ arc-shaped insole surface

13‧‧‧岐管 13‧‧‧岐管

131‧‧‧輸出口 131‧‧‧Outlet

132‧‧‧輸入口 132‧‧‧ input port

14‧‧‧曝氣裝置 14‧‧‧Aeration device

15‧‧‧樞接件 15‧‧‧ pivotal parts

2‧‧‧培養液 2‧‧‧ culture solution

3‧‧‧光源 3‧‧‧Light source

C‧‧‧容置空間 C‧‧‧ accommodating space

D1‧‧‧寬度 D1‧‧‧Width

D2‧‧‧管徑 D2‧‧‧ pipe diameter

H1,H2‧‧‧高度 H1, H2‧‧‧ height

H3‧‧‧總高度 H3‧‧‧ total height

H4‧‧‧距離 H4‧‧‧ distance

圖1為本揭露實施例1之生物反應系統之示意圖。 1 is a schematic view of a biological reaction system of Example 1.

圖2為本揭露實施例2之生物反應系統之示意圖。 2 is a schematic view of a biological reaction system of Example 2.

圖3為本揭露實施例3之生物反應系統之示意圖。 3 is a schematic view of a biological reaction system of Example 3.

圖4為本揭露比較例之生物反應系統之示意圖。 4 is a schematic view of a biological reaction system of a comparative example.

圖5為本揭露測試例之使用比較例之生物反應系統之實驗結果圖。 Fig. 5 is a graph showing experimental results of a biological reaction system using a comparative example of the test example.

圖6為本揭露測試例之使用實施例1之生物反應系統之實驗結果圖。 Fig. 6 is a graph showing experimental results of the biological reaction system of Example 1 using the test example.

以下係藉由特定的具體實施例說明本揭露之實施方式,熟習此技藝之人士可由本說明書所揭示之內容輕易地了解本揭露之其他優點與功效。本揭露亦可藉由其他不同的具體實施例加以施行或應用,本說明書中的各項細節亦可針對不同觀點與應用,在不悖離本創作之精神下進行各種修飾與變更。 The embodiments of the present disclosure are described by way of specific examples, and those skilled in the art can readily appreciate the other advantages and advantages of the disclosure. The disclosure may also be implemented or applied by other different embodiments. The details of the present specification may also be applied to various aspects and applications, and various modifications and changes may be made without departing from the spirit of the present invention.

實施例1Example 1

圖1為本實施例之生物反應系統之示意圖。如圖1所示,本實施例之生物反應系統是為一種氣舉式生物反應系統,其包括:一管柱11,包括:一管柱頂端111、一管柱底端112及一管柱側部113,其中管柱側部113設於管柱頂端111及管柱底端112間且與管柱頂端111及管柱底端112相連;一底部12,設於管柱11之管柱底端112,管柱11與底部12形成一容置空間C,且底部12具有一類弧型內底表面121;一岐管13,設於管柱11外且具有一輸出口131及複數輸入口132(於本實施例中,為兩個輸入口132),輸出口131與底部12連接,而輸入口132與管柱側部113連接;一曝氣裝置14,與管柱頂端111連接以提供一氣體至容置空間C。 Figure 1 is a schematic illustration of the biological reaction system of the present embodiment. As shown in FIG. 1 , the biological reaction system of the present embodiment is a gas lift bioreactor system comprising: a column 11 comprising: a column top end 111, a column bottom end 112 and a column side a portion 113, wherein the column side portion 113 is disposed between the top end 111 of the column and the bottom end 112 of the column and connected to the top end 111 of the column and the bottom end 112 of the column; a bottom portion 12 is disposed at the bottom end of the column of the column 11 112, the column 11 and the bottom portion 12 form an accommodating space C, and the bottom portion 12 has a curved inner bottom surface 121; a manifold 13 is disposed outside the column 11 and has an output port 131 and a plurality of input ports 132 ( In the present embodiment, there are two input ports 132), the output port 131 is connected to the bottom portion 12, and the input port 132 is connected to the column side portion 113; an aeration device 14 is connected to the column top end 111 to provide a gas. To accommodate space C.

此外,本實施例之生物反應系統可更包括一上蓋114,設於管柱11之管柱頂端111,以封閉管柱頂端111。 In addition, the biological reaction system of the present embodiment may further include an upper cover 114 disposed at the top end 111 of the column of the column 11 to close the top end 111 of the column.

再者,於本實施例之生物反應系統中,管柱11與底部12為兩個獨立的元件,其藉由一樞接件15連接。當管柱11與底部12為兩個獨立的元件時,可達到容易清洗管柱11與底部12之目的。 Furthermore, in the biological reaction system of the present embodiment, the column 11 and the bottom portion 12 are two separate elements connected by a pivoting member 15. When the column 11 and the bottom 12 are two separate components, the purpose of easily cleaning the column 11 and the bottom 12 can be achieved.

如圖1所示,當一培養液2係設於容置空間C中時,且當曝氣裝置14提供氣體至容置空間C中時,培養液2係由岐管13的輸出口131流出並通過岐管13而由輸入口132循環至容置空間C中,如箭號所示,而可帶動水流造成漩渦,增加整個培養系統的循環。 As shown in FIG. 1, when a culture solution 2 is disposed in the accommodating space C, and when the aeration device 14 supplies gas into the accommodating space C, the broth 2 is discharged from the output port 131 of the manifold 13 and It is circulated through the inlet port 132 into the accommodating space C through the manifold 13, as indicated by the arrow, which can drive the water flow to cause a vortex, which increases the circulation of the entire culture system.

於本實施例之生物反應系統中,底部12具有一類弧型內底表面121;更具體而言,此類弧型內底表面121為一弧型內底表面。於本實施例中,類弧型內底表面121為一半圓形內底表面。然而,本揭露之其他實施例並不僅限於此,只要底部12具有一類弧型內底表面121,較佳為一弧型內底表面。於本實施例之生物反應系統中,因底部12具有一類弧型內底表面121,故不會有死角產生;如此,則可避免培養液2循環時被培養物沉積於死角的情形,進而提升被培養物的生長效率。 In the bioreactor system of the present embodiment, the bottom portion 12 has a curved inner bottom surface 121; more specifically, the curved inner bottom surface 121 is an arcuate inner bottom surface. In the present embodiment, the arc-like inner bottom surface 121 is a semi-circular inner bottom surface. However, other embodiments of the present disclosure are not limited thereto, as long as the bottom portion 12 has a curved inner bottom surface 121, preferably an arcuate inner bottom surface. In the biological reaction system of the present embodiment, since the bottom portion 12 has a type of arc-shaped inner bottom surface 121, no dead angle is generated; thus, the culture body can be prevented from being deposited at a dead angle when the culture liquid 2 is circulated, thereby improving The growth efficiency of the culture.

此外,於本實施例之生物反應系統中,管柱11為一圓柱形空心管柱。 Further, in the biological reaction system of the present embodiment, the column 11 is a cylindrical hollow column.

如圖1所示,本實施例之生物反應系統可更包括一光源3,設於容置空間C中。當光源3設於容置空間C時,可使被培養物能夠均勻照光,進而提升被培養物的生長效率。 As shown in FIG. 1 , the biological reaction system of the present embodiment further includes a light source 3 disposed in the accommodating space C. When the light source 3 is disposed in the accommodating space C, the cultured object can be uniformly illuminated, thereby improving the growth efficiency of the cultured object.

此外,於本實施例之生物反應系統中,管柱11、底部12及岐管13之材料並無特殊限制,例如:玻璃、壓克力材質、其他塑膠材質、或透光材質。較佳為,管柱11、底部12及岐管13之材料為一壓克力之透光材質。 In addition, in the biological reaction system of the embodiment, the materials of the column 11, the bottom 12 and the manifold 13 are not particularly limited, for example, glass, acrylic, other plastic materials, or light-transmitting materials. Preferably, the material of the column 11, the bottom 12 and the manifold 13 is a acryl transparent material.

再者,本實施例之生物反應系統之尺寸並無特殊限制,只要能夠達到氣舉式循環的目的即可。為了於底部12達到較佳的循環效果,底部12於管柱11之一長度方向上具有一高度H1,底部12於垂直管柱11之該長度方向上具有一寬度D1,而高度H1與寬度D1比係介於0.5至4.0之間。於本實施例中,底部12為一半圓形底部,其高度H1為15cm,而寬度D1(也可說是直徑)為20cm。 Furthermore, the size of the biological reaction system of the present embodiment is not particularly limited as long as the purpose of the gas lift cycle can be achieved. In order to achieve a better circulation effect on the bottom portion 12, the bottom portion 12 has a height H1 in the longitudinal direction of one of the columns 11, and the bottom portion 12 has a width D1 and a height H1 and a width D1 in the longitudinal direction of the vertical column 11. The ratio is between 0.5 and 4.0. In the present embodiment, the bottom portion 12 is a semicircular bottom having a height H1 of 15 cm and a width D1 (also arguably a diameter) of 20 cm.

於本實施例之生物反應系統中,管柱11的尺寸並無特殊限制,只要能與底部12匹配即可。於本實施例中,管柱11的高度H2為110cm,而寬度(等同於底部12之寬度D1)為20cm。因此,管柱11與底部12的總高度H3為125cm。 In the biological reaction system of the present embodiment, the size of the column 11 is not particularly limited as long as it can match the bottom portion 12. In the present embodiment, the height H2 of the column 11 is 110 cm, and the width (equivalent to the width D1 of the bottom 12) is 20 cm. Therefore, the total height H3 of the column 11 and the bottom portion 12 is 125 cm.

此外,為了達到較佳的氣舉循環效果,岐管13之輸出口131與輸入口132於管柱11之一長度方向上的距離H4係介於50cm至300cm之間。若距離H4大於200cm,則無法達到良好的氣舉循環目的。 In addition, in order to achieve a better gas lift cycle effect, the distance H4 between the output port 131 of the manifold 13 and the input port 132 in the longitudinal direction of the column 11 is between 50 cm and 300 cm. If the distance H4 is greater than 200 cm, a good gas lift cycle purpose cannot be achieved.

再者,於本實施例之生物反應系統中,岐管13的管徑D2並無特殊限制;於本實施例中,岐管13的管徑D2為2cm。 Further, in the biological reaction system of the present embodiment, the diameter D2 of the manifold 13 is not particularly limited; in the present embodiment, the diameter D2 of the manifold 13 is 2 cm.

當使用本實施例之生物反應系統培養被培養物時,例如培養含葉黃素微藻、含油微藻、或其他微藻,可有效增加反應系統內的培養液循環;同時,也可用於二氧化碳減量,生產微藻生物質。 When the cultured body is cultured using the biological reaction system of the present embodiment, for example, culturing the lutein-containing microalgae, the oil-containing microalgae, or other microalgae can effectively increase the circulation of the culture solution in the reaction system; and at the same time, it can also be used for carbon dioxide reduction. Production of microalgal biomass.

實施例2Example 2

如圖2所示,本實施例之生物反應系統與實施例1相同,除了管柱11及底部12係一體成形。 As shown in Fig. 2, the biological reaction system of this embodiment is the same as that of the first embodiment except that the column 11 and the bottom portion 12 are integrally formed.

實施例3Example 3

如圖3所示,本實施例之生物反應系統與實施例1相同,除了光源3(在此,係以太陽光做舉例)是設於管柱11外。 As shown in FIG. 3, the biological reaction system of the present embodiment is the same as that of Embodiment 1, except that the light source 3 (here, exemplified by sunlight) is disposed outside the column 11.

比較例Comparative example

如圖4所示,本比較例之生物反應系統與實施例3相同,除了本比較例之生物反應系統不包括岐管13,且管柱11之底部115為一平面,而與實施例3之半圓形底部12(如圖3所示)不同。 As shown in FIG. 4, the biological reaction system of the present comparative example is the same as that of the third embodiment except that the biological reaction system of the comparative example does not include the manifold 13, and the bottom 115 of the column 11 is a flat surface, and is the same as that of the third embodiment. The semicircular bottom 12 (shown in Figure 3) is different.

測試例Test case

於本測試例中,係使用實施例1及比較例所示之生物反應系統,透過曝氣裝置通入二氧化碳,以培養含葉黃素微藻。其中,將含葉黃素微藻(Chlorella sorokiniana)(藻體起始濃度0.05g/L)加入至基礎培養液(Basal medium)中,以2.0%、0.05vvm的二氧化碳曝氣量進行培養,其中,該基礎培養液包括有:硝酸鉀(氮源)以及磷源。 In the present test example, the bioreactor system shown in Example 1 and the comparative example was used, and carbon dioxide was introduced through the aeration device to culture the lutein-containing microalgae. Wherein, the chlorophyll- containing microalgae ( Chlorella sorokiniana ) (the initial concentration of the algae body is 0.05 g/L) is added to the base broth (Basal medium), and the cultivar is cultured at a carbon dioxide aeration rate of 2.0% and 0.05 vvm, wherein The base culture solution includes: potassium nitrate (nitrogen source) and a phosphorus source.

圖5為使用比較例所示之生物反應系統之實驗結果圖,而圖6為使用實施例1所示之生物反應系統之實驗結果圖。此外,並依下列式(I)計算葉黃素的產率:葉黃素產率=藻體重量x葉黃素含量/藻體培養天數 (I) Fig. 5 is a graph showing the results of experiments using the bioreactor system shown in the comparative example, and Fig. 6 is a graph showing the results of experiments using the bioreactor system shown in Example 1. In addition, the yield of lutein was calculated according to the following formula (I): lutein yield = algal body weight x lutein content / algae culture days (I)

如圖5及圖6所示,相較於比較例之氣舉式生物反應系統,當使用本揭露實施例1之生物反應系統培養含葉黃素微藻時,葉黃素的產率可由1.74mg/l/d提升至2.32mg/l/d,產率提升35.6%。此結果證實,本揭露之生物反應系統,透過具有類弧型內底表面之底部,而確實可提升被培養物的產率。 As shown in FIG. 5 and FIG. 6 , when the lutein-containing microalgae is cultured using the biological reaction system of the first embodiment of the present invention, the yield of lutein can be 1.74 mg/in comparison with the gas lift bioreactor system of the comparative example. The l/d was increased to 2.32 mg/l/d, and the yield was increased by 35.6%. This result confirms that the bioreactor system of the present disclosure, through having the bottom of the arc-like inner bottom surface, does enhance the yield of the culture.

上述實施例僅係為了方便說明而舉例而已,本揭露所主張之權利範圍自應以申請專利範圍所述為準,而非僅限於上述實施例。 The above-mentioned embodiments are merely examples for convenience of description, and the scope of the claims is intended to be limited to the above embodiments.

Claims (8)

一種生物反應系統,包括:一管柱,包括:一管柱頂端、一管柱底端及一管柱側部,其中該管柱側部設於該管柱頂端及該管柱底端間且與該管柱頂端及該管柱底端相連;一底部,設於該管柱之該管柱底端,該管柱與該底部形成一容置空間,且該底部具有一弧型內底表面;一岐管,設於該管柱外且具有一輸出口及複數輸入口,該輸出口與該底部連接,而該些輸入口與該管柱側部連接,且該輸出口與該些輸入口於該管柱之一長度方向上的距離係介於50cm至300cm之間;一曝氣裝置,與該管柱頂端連接以提供一氣體至該容置空間;其中,該底部於該管柱之一長度方向上具有一高度,該底部於垂直該管柱之該長度方向上具有一寬度,而該高度與該寬度比係介於0.5至4.0之間。 A biological reaction system comprising: a column comprising: a column top end, a column bottom end and a column side, wherein the column side is disposed between the top of the column and the bottom end of the column and Connected to the top of the column and the bottom end of the column; a bottom portion is disposed at the bottom end of the column of the column, the column forms an accommodation space with the bottom, and the bottom has an arc-shaped inner bottom surface a manifold, disposed outside the column and having an output port and a plurality of input ports, the output port being connected to the bottom portion, wherein the input ports are connected to the side of the column column, and the output port and the inputs are The distance between the length of one of the columns is between 50cm and 300cm; an aeration device is connected to the top of the column to provide a gas to the accommodating space; wherein the bottom is at the column One of the lengths has a height, and the bottom has a width in the longitudinal direction of the vertical string, and the height is proportional to the width between 0.5 and 4.0. 如申請專利範圍第1項所述之生物反應系統,其中該管柱及該底部係透過一樞接件連接。 The biological reaction system of claim 1, wherein the column and the bottom are connected by a pivoting member. 如申請專利範圍第1項所述之生物反應系統,其中該管柱及該底部係一體成形。 The biological reaction system of claim 1, wherein the column and the bottom are integrally formed. 如申請專利範圍第1項所述之生物反應系統,更包括一光源,設於該管柱外或該容置空間中。 The biological reaction system of claim 1, further comprising a light source disposed outside the column or in the accommodating space. 如申請專利範圍第4項所述之生物反應系統,其中該光源設於該容置空間中。 The biological reaction system of claim 4, wherein the light source is disposed in the accommodating space. 如申請專利範圍第1項所述之生物反應系統,其中該弧型內表面係為一半圓形內底表面。 The biological reaction system of claim 1, wherein the curved inner surface is a semicircular inner bottom surface. 如申請專利範圍第1項所述之生物反應系統,其中一培養液係設於該容置空間中;且當該曝氣裝置提供該氣體至該容置空間中時,該培養液係由該輸出口流出並通過該岐管而由該些輸入口循環至該容置空間中。 The biological reaction system of claim 1, wherein a culture solution is disposed in the accommodating space; and when the aeration device supplies the gas into the accommodating space, the broth is The output port flows out through the manifold and is circulated into the accommodating space by the input ports. 如申請專利範圍第1項所述之生物反應系統,其中該管柱為一圓柱形空心管柱。 The biological reaction system of claim 1, wherein the column is a cylindrical hollow column.
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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010051371A1 (en) * 1998-02-27 2001-12-13 Dale Vilmer Kiplinger Automated biogenerator
WO2009149519A1 (en) * 2008-06-12 2009-12-17 Winwick Business Solutions Pty Ltd System for cultivation and processing of microorganisms and products therefrom
WO2011069372A1 (en) * 2009-12-10 2011-06-16 中国科学院广州能源研究所 Photobioreactor system for high-density culture of microalgae

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010051371A1 (en) * 1998-02-27 2001-12-13 Dale Vilmer Kiplinger Automated biogenerator
WO2009149519A1 (en) * 2008-06-12 2009-12-17 Winwick Business Solutions Pty Ltd System for cultivation and processing of microorganisms and products therefrom
WO2011069372A1 (en) * 2009-12-10 2011-06-16 中国科学院广州能源研究所 Photobioreactor system for high-density culture of microalgae

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