TWI579377B

TWI579377B - Fixtures

Info

Publication number: TWI579377B
Application number: TW104125535A
Authority: TW
Inventors: Geng-Liang Ou
Priority date: 2015-08-06
Filing date: 2015-08-06
Publication date: 2017-04-21
Also published as: TW201706404A; CN106434273A; CN106434273B; CN205275566U

Description

固定裝置Fixtures

本創作係提供一種固定裝置，特別係一種用於固定培養細胞玻璃片之固定裝置。The present invention provides a fixation device, in particular a fixation device for fixing a glass plate for culturing cells.

細胞培養技術係生物科技領域之核心技術，利用人工培養的方法在生物體外進行大量的細胞培養，可以應用於疾病之診斷、新藥之藥效評估、動物檢查、植物檢查或環境污染物質之試驗等。The cell culture technology is the core technology in the field of biotechnology. It uses artificial culture to carry out a large number of cell culture in vitro. It can be applied to the diagnosis of diseases, the evaluation of new drugs, animal inspection, plant inspection or environmental pollutants. .

而人工培養之細胞通常係於細胞培養容器中進行，一般之細胞培養容器係有塑膠製培養皿、玻璃製培養皿或井板(well plate)等，其中井板依其井數又可分為6孔井、12孔井、48孔井和96孔井等不同之種類。如圖5所示，其係為一6孔井80，該6孔井80包含六培養井81，而人工培養的方法係先將複數玻璃片(coverslips)90分別置入各培養井81內，並將欲培養之細胞置於該等玻璃片90上，再加入培養液於各培養井81內，接著定期更換各培養井81內之培養液即可完成細胞之培養。The artificially cultured cells are usually carried out in a cell culture vessel. The general cell culture vessel is a plastic culture dish, a glass culture dish or a well plate, etc., wherein the well plate can be divided into several wells according to the number of wells. Different types of 6-well, 12-well, 48-well and 96-well wells. As shown in FIG. 5, it is a 6-well well 80, which includes six culture wells 81. The artificial culture method firstly inserts a plurality of cover sheets 90 into each culture well 81. The cells to be cultured are placed on the glass sheets 90, and the culture solution is added to each of the culture wells 81, and then the culture medium in each of the culture wells 81 is periodically replaced to complete the culture of the cells.

但如圖6所示，而於移動該6孔井80時之晃動容易造成該等玻璃片90漂浮於培養液之液面，如此將使得附著於該等玻璃片90相對於液面之細胞無法持續浸泡於培養液中，進而導致細胞繁殖速度降低，甚至造成細胞的死亡。However, as shown in FIG. 6, the shaking of the 6-well well 80 tends to cause the glass sheets 90 to float on the liquid surface of the culture solution, so that the cells attached to the glass sheets 90 relative to the liquid surface cannot be Continuous soaking in the culture solution leads to a decrease in the rate of cell proliferation and even death of the cells.

本創作之目的在於改善玻璃片漂浮於培養液之液面之情況，以減少細胞繁殖速度降低或細胞死亡的可能性。The purpose of this creation is to improve the condition that the glass flakes float on the liquid level of the culture solution to reduce the possibility of cell proliferation or cell death.

本創作提供一種固定裝置，其包含：一中空柱體，其具有一容置空間、一頂面、一底面與一內面，該容置空間貫穿成型於該中空柱體，該頂面位於該中空柱體之頂部，該底面位於該中空柱體之底部並與該頂面之位置相對，該內面位於頂面與該底面之間並面向該容置空間；以及複數凸設塊，其設於該中空柱體之內面，而該等凸設塊係間隔排列並位於同一水平面上，且該等凸設塊中其中一凸設塊係與該等凸設塊中另一凸設塊之位置相對。The present invention provides a fixing device, comprising: a hollow cylinder having an accommodating space, a top surface, a bottom surface and an inner surface, the accommodating space being formed through the hollow cylinder, wherein the top surface is located a top portion of the hollow cylinder, the bottom surface is located at a bottom of the hollow cylinder and opposite to a position of the top surface, the inner surface is located between the top surface and the bottom surface and faces the receiving space; and a plurality of protruding blocks are provided On the inner surface of the hollow cylinder, the protruding blocks are arranged at intervals and located on the same horizontal surface, and one of the protruding blocks and the other protruding block of the protruding blocks Relative position.

本創作使用時，係將該固定裝置置入培養井中，並使玻璃片容置於該容置空間中，藉由該等凸設塊將玻璃片壓抵於培養液中，以達到避免玻璃片漂浮於培養液之液面之目的，並以此減少細胞繁殖速度降低或細胞死亡的可能性。In the present application, the fixing device is placed in the culture well, and the glass piece is placed in the accommodating space, and the glass piece is pressed against the culture liquid by the protruding blocks to avoid the glass piece. Floating on the surface of the culture solution, and thereby reducing the possibility of decreased cell growth rate or cell death.

較佳的是，其中該中空柱體更具有複數凹槽，該等凹槽由該底面朝向該頂面凹設成型，且該等凹槽係間隔排列並與該容置空間相連通，且該等凹槽之底面與中空柱體之頂面之間形成有一間距。該等凹槽亦提供培養液循環流通之通道。Preferably, the hollow cylinder further has a plurality of grooves, the grooves are concavely formed by the bottom surface toward the top surface, and the grooves are spaced apart and communicate with the accommodating space, and A space is formed between the bottom surface of the grooves and the top surface of the hollow cylinder. The grooves also provide access to the circulation of the culture fluid.

較佳的是，其中該等凹槽係與該等凸設塊間呈交錯排列。Preferably, the grooves are staggered with the protruding blocks.

較佳的是，其中該等凸設塊於位置上相對於該等凹槽更靠近該中空柱體之頂面。Preferably, the protruding blocks are located closer to the top surface of the hollow cylinder relative to the grooves.

較佳的是，其中各凸設塊具有相連接之一連接端與一自由端，各凸設塊之連接端係設於該中空柱體之內面，該自由端位於該凸設塊於位置上相對於該內面之一端，且該自由端上凹設成型一內凹部，該內凹部係面向該中空柱體之頂面。該內凹部主要係用於避免於添加培養液時，因液體流動導致玻璃片之移動，進而減緩或抑制細胞之生長。該內凹部之尺寸亦得依所使用之玻璃片的大小與形狀做調整與設計。Preferably, each of the protruding blocks has a connecting end and a free end, and the connecting end of each protruding block is disposed on the inner surface of the hollow cylinder, and the free end is located at the protruding block. An inner recess is formed on the free end, and the inner recess is recessed to face the top surface of the hollow cylinder. The concave portion is mainly used to avoid the movement of the glass sheet due to the liquid flow when the culture liquid is added, thereby slowing down or inhibiting the growth of the cells. The size of the recessed portion is also adjusted and designed according to the size and shape of the glass piece to be used.

較佳的是，其中該中空柱體之橫切面呈圓形、矩形或三角形。Preferably, the cross section of the hollow cylinder is circular, rectangular or triangular.

如圖1所示，本創作提供一種固定裝置1，其包含：一中空柱體10與複數凸設塊20。As shown in FIG. 1, the present invention provides a fixing device 1 comprising: a hollow cylinder 10 and a plurality of protruding blocks 20.

該中空柱體10具有一容置空間11、一頂面12、一底面13、一內面14與複數凹槽15；該容置空間11貫穿成型於該中空柱體10；該頂面12位於該中空柱體10之頂部；該底面13位於該中空柱體10之底部並與該頂面12之位置相對；該內面14位於頂面12與該底面13之間並面向該容置空間11；該等凹槽15由該底面13朝向該頂面12凹設成型，且該等凹槽15係間隔排列並與該容置空間11相連通，且該等凹槽15之底面151與中空柱體10之頂面12之間形成有一間距。The hollow cylinder 10 has an accommodating space 11, a top surface 12, a bottom surface 13, an inner surface 14 and a plurality of grooves 15. The accommodating space 11 is formed through the hollow cylinder 10; the top surface 12 is located a top portion of the hollow cylinder 10; the bottom surface 13 is located at a bottom of the hollow cylinder 10 and opposite to the top surface 12; the inner surface 14 is located between the top surface 12 and the bottom surface 13 and faces the receiving space 11 The grooves 15 are recessed from the bottom surface 13 toward the top surface 12, and the grooves 15 are spaced apart and communicate with the accommodating space 11, and the bottom surface 151 and the hollow surface of the grooves 15 are hollow. A spacing is formed between the top faces 12 of the cylinders 10.

配合圖2所示，該等凸設塊20設於該中空柱體10之內面14，而該等凸設塊20係間隔排列並位於同一水平面上，且該等凸設塊20中其中一凸設塊20係與該等凸設塊20中另一凸設塊20之位置相對，各凸設塊20具有相連接之一連接端21與一自由端22，各凸設塊20之連接端21係設於該中空柱體10之內面14，該自由端22位於該凸設塊20於位置上相對於該內面14之一端，且該自由端22上凹設成型一內凹部221，該內凹部221係面向該中空柱體10之頂面12。As shown in FIG. 2, the protruding blocks 20 are disposed on the inner surface 14 of the hollow cylinder 10, and the protruding blocks 20 are spaced and arranged on the same horizontal surface, and one of the protruding blocks 20 is The protruding block 20 is opposite to the position of the other protruding block 20 of the protruding blocks 20, and each protruding block 20 has a connecting end 21 and a free end 22, and the connecting ends of the protruding blocks 20 21 is disposed on the inner surface 14 of the hollow cylinder 10, the free end 22 is located at one end of the protruding block 20 relative to the inner surface 14, and the inner end 14 is recessed and formed on the free end 22 The inner recess 221 faces the top surface 12 of the hollow cylinder 10.

配合圖2所示，具體而言，該中空柱體10係呈圓筒狀，即該中空柱體10之橫切面呈圓形，而該等凸設塊20之數量為四個，該等凸設塊20之位置係兩兩相對設置，該等凹槽15之數量為四個，該等凹槽15係與該等凸設塊20間呈交錯排列，且該等凹槽15之位置係兩兩相對設置，換言之，任兩相鄰之凸設塊20間僅具有該等凹槽15中的其中一凹槽15；配合圖3所示，該等凸設塊20於位置上相對於該等凹槽15更靠近該中空柱體10之頂面12。As shown in FIG. 2 , specifically, the hollow cylinder 10 is cylindrical, that is, the cross section of the hollow cylinder 10 is circular, and the number of the protruding blocks 20 is four, and the convex The position of the block 20 is oppositely arranged. The number of the grooves 15 is four, and the grooves 15 are staggered with the protruding blocks 20, and the positions of the grooves 15 are two. Two opposite arrangements, in other words, between any two adjacent protruding blocks 20, only one of the grooves 15 is provided; as shown in FIG. 3, the protruding blocks 20 are positionally opposed to the same The groove 15 is closer to the top surface 12 of the hollow cylinder 10.

配合圖4所示，本創作之固定裝置1應用於細胞培養時，係將該固定裝置1置入培養井81內，並使玻璃片90容置於該容置空間11中，透過該等凸設塊20將玻璃片90壓抵於培養液中，以避免玻璃片90漂浮於培養液之液面，藉此減少細胞繁殖速度降低或細胞死亡的可能性；而該等凹槽15亦提供培養液循環流通之通道。As shown in FIG. 4, when the fixing device 1 of the present invention is applied to cell culture, the fixing device 1 is placed in the culture well 81, and the glass sheet 90 is received in the accommodating space 11 through the convex Block 20 presses the glass sheet 90 against the culture solution to prevent the glass sheet 90 from floating on the liquid level of the culture solution, thereby reducing the possibility of a decrease in cell proliferation rate or cell death; and the grooves 15 also provide culture The passage of liquid circulation.

以上所述僅為說明本創作的例示，並非對本創作做任何形式上的限制，本創作所主張之權利範圍自應以申請專利範圍所述為準，而非僅限於上述實施例。任何所屬技術領域中具有通常知識者，在不脫離本創作技術方案的範圍內，當可利用上述揭示的技術內容做出些許更動或修飾為等同變化的等效實施例，但凡是未脫離本創作之技術方案的內容，依據本創作的技術實質對以上實施例作任何簡單修改、等同變化與修改，均仍屬於本創作技術方案的範圍內。The above description is only illustrative of the present invention, and is not intended to limit the scope of the present invention. The scope of the present invention is defined by the scope of the patent application, and is not limited to the above embodiments. Any equivalents of the above-disclosed technical contents may be modified or modified to equivalent variations, without departing from the scope of the present invention. The content of the technical solution, any simple modification, equivalent change and modification of the above embodiment according to the technical essence of the present invention are still within the scope of the technical solution of the present invention.

1固定裝置 10中空柱體 11容置空間 12頂面 13底面 14內面 15凹槽 151底面 20凸設塊 21連接端 22自由端 221內凹部 80 6孔井 81培養井 90玻璃片1 Fixing device 10 Hollow cylinder 11 accommodating space 12 top surface 13 bottom surface 14 inner surface 15 groove 151 bottom surface 20 protruding block 21 connecting end 22 free end 221 inner recess 80 6 hole well 81 culture well 90 glass piece

圖1為本創作之固定裝置的外觀圖。圖2為本創作之固定裝置的俯視圖。圖3為本創作之固定裝置的剖面圖。圖4為本創作之固定裝置應用於培養井中玻璃片的剖面示意圖。圖5為現有技術中6孔井與玻璃片之外觀示意圖。圖6為現有技術中玻璃片漂浮於培養井中培養液的液面之剖面示意圖。Figure 1 is an external view of the fixture of the present invention. Figure 2 is a top plan view of the fixture of the present invention. Figure 3 is a cross-sectional view of the fixture of the present invention. Fig. 4 is a schematic cross-sectional view showing the fixing device of the present invention applied to a glass piece in a culture well. Fig. 5 is a schematic view showing the appearance of a 6-well well and a glass piece in the prior art. Fig. 6 is a schematic cross-sectional view showing a liquid surface of a culture medium in which a glass piece floats in a culture well in the prior art.

1固定裝置 11容置空間 15凹槽 20凸設塊 81培養井 90玻璃片1 Fixing device 11 accommodating space 15 groove 20 protruding block 81 culture well 90 glass piece

Claims

一種固定裝置，其包含：一中空柱體，其具有一容置空間、一頂面、一底面、一內面及複數凹槽，該容置空間貫穿成型於該中空柱體，該頂面位於該中空柱體之頂部，該底面位於該中空柱體之底部並與該頂面之位置相對，該內面位於頂面與該底面之間並面向該容置空間，該等凹槽由該底面朝向該頂面凹設成型，且該等凹槽係間隔排列並與該容置空間相連通，且該等凹槽之底面與中空柱體之頂面之間形成有一間距；以及複數凸設塊，其設於該中空柱體之內面，而該等凸設塊係間隔排列並位於同一水平面上，且該等凸設塊中其中一凸設塊係與該等凸設塊中另一凸設塊之位置相對。 A fixing device comprising: a hollow cylinder having an accommodating space, a top surface, a bottom surface, an inner surface and a plurality of grooves, the accommodating space being formed through the hollow cylinder, the top surface being located a top surface of the hollow cylinder, the bottom surface is located at a bottom of the hollow cylinder and opposite to a position of the top surface, the inner surface is located between the top surface and the bottom surface and faces the accommodating space, the grooves are formed by the bottom surface Formed toward the top surface, and the grooves are spaced apart and communicate with the accommodating space, and a space is formed between the bottom surface of the grooves and the top surface of the hollow cylinder; and a plurality of protrusions a block disposed on an inner surface of the hollow cylinder, wherein the protruding blocks are spaced apart and located on the same horizontal plane, and one of the protruding blocks and the other of the protruding blocks The positions of the protruding blocks are opposite.

依據請求項1所述之固定裝置，其中該等凹槽係與該等凸設塊間呈交錯排列。 The fixing device according to claim 1, wherein the grooves are staggered with the protruding blocks.

依據請求項1所述之固定裝置，其中該等凸設塊於位置上相對於該等凹槽更靠近該中空柱體之頂面。 The fixing device of claim 1, wherein the protruding blocks are positioned closer to a top surface of the hollow cylinder relative to the grooves.

依據請求項2所述之固定裝置，其中該等凸設塊於位置上相對於該等凹槽更靠近該中空柱體之頂面。 The fixing device of claim 2, wherein the protruding blocks are positioned closer to a top surface of the hollow cylinder relative to the grooves.

依據請求項1至4中任一項所述之固定裝置，其中各凸設塊具有相連接之一連接端與一自由端，各凸設塊之連接端係設於該中空柱體之內面，該自由端位於該凸設塊於位置上相對於該內面之一端，且該自由端上凹設成型一內凹部，該內凹部係面向該中空柱體之頂面。 The fixing device according to any one of claims 1 to 4, wherein each of the protruding blocks has a connecting end and a free end, and the connecting end of each protruding block is disposed on the inner surface of the hollow cylinder. The free end is located at one end of the protruding block relative to the inner surface, and the free end is recessed to form an inner recess facing the top surface of the hollow cylinder.

依據請求項1至4中任一項所述之固定裝置，其中該中空柱體之橫切面呈圓形、矩形或三角形。The fixing device according to any one of claims 1 to 4, wherein the hollow cylinder has a circular, rectangular or triangular cross section.