1300336 v 九、發明說明： 【發明所屬之技術領域】 ' 本發明是有關於一種即溶全吸收珍珠粉的製造方法，特 別是有關於一種具有製造過程中完全保留原珍珠粉之活性成 分與縮短製程時間之珍珠粉的製造方法。 【先前技術】 珍珠粉經實驗分析證明富含各種氨基酸（如賴氨酸、色 氨酸、苯丙氨酸、甲硫氨酸、蘇氨酸、亮氨酸、異亮氨酸、和 纈氨酸等）、微量元素（如鋅Zn、硒Se、鎂Mg、鍺Ge等）、有機 生物鈣及小分子多胜肽等；在本草綱目中記載著··「〔氣味〕珍 珠粉鹹甘、寒、無毒；〔主治〕鎮心、安魂魄、磨翳墜痰、止 痛、療煩熱消渴、防止遺精白濁、主難產下死胎胞衣、令人潤 澤好顏色、去皮膚逆臚、去翳障全聾、解痘疔毒」，故由古至 今珍珠粉即為國人之養生聖品。 於先前技術中，珍珠粉依傳統方法係以牛奶泡製及豆腐 蒸煮後，再以水飛法取得細微的粉末。今日，有許多人仍以此 方法製備珍珠粉，或者以改善研磨方式或技術的方法，並用真 空的方式乾燥珍珠粉，但這類珍珠粉溶解度有限，無法完全為 人體吸收利用。1991年王四明等人提出「水溶性全成分珍珠粉 系列產品新製備方法」（中國專利申請號90109538.9)，其製造 方法是用複合羧酸（含丙酸、醋酸等）先水解分離得珍珠複合 1300336 羧酸鹽類溶液及珍珠硬蛋白，再以6卷1、曲 與酵素水解珍珠硬蛋白後;混合珍“合緩酸酸= 攝氏60度真空濃縮蒸發器内乾燥而成。 w液並在 然而，先前技術具有無法避免之缺點，使人 水解珍珠粉，又以鹽酸處理珍珠硬蛋白， 妓内夂 份的氨基酸會產生破壞，而 由於其使用高溫濃縮乾燥以及使用古、曲 、、★土 ·文 ^、並明朝糾輯著林财巾所^珍珠粉為械 甘寒、無毒的特性。 【發明内容】 有鐘於此，為解決上述問題，本發明係提供—種即溶全 =珍珠粉的製造方法’其目的絲造過財完全保留原珍 珠籾之活性成分與縮短製程時間的製造方法。 本發明所揭露即溶全吸收珍珠粉的製造方法中，採用分 P皆段進行魏浸_序、豆絲綠豆蒸結序、細碎化程序、 酵素分解程序、固形物水解㈣與真空;東結乾燥程序。 …第—階段的鮮乳浸泡程序’於珍珠原料中加入鮮乳，待 鮮乳與珍珠原料浸泡—段時間後生成第—珍珠生成物，進入 至第二階段。 第二階段的豆腐和綠豆蒸煮程序，㈣—珍珠生成物中 加入豆腐和綠豆，均勻混合後加熱《後生成第二珍珠生成 物，進入至第三階段。 •1300336 第三階段的細碎化程序，將第二珍珠生成物中放入粉碎 機與球磨機細碎成細碎珍珠粉末生成物，進入至第四階段。 第四階段的酵素分解程序，將細碎珍珠粉末生成物中加 入純水、蛋白水解酵素與N端胜肽分解酶充份混合進行反 應，生成珍珠胺基酸生成物、珍珠胜肽生成物、微量 合物與沉澱固形物。 用 第五階段的固形物水解程序，將沉澱固形物中加入人 ;===乾;有機生物_液： 珍珠胜肽生成物'微量元素混:==生成物、 本發明之有益功致為==物即溶全吸收珍珠粉。 方法，本即溶全讀以粉㈣^溶全缝珍珠粉的製造 序，將珍珠粉的製造程序時間法改良珍珠粉的製造程 故無破壞珍珠粉的營養活性成八短不使用鹽酸等強酸處理， 維奈米等級，具有印溶易於77且製成之珍珠粉顆粒達_ 程，不會造成環境的負擔二，其為綠色環保的製 成本、等諸多功效。 /、衣程間單、降低製造工時與 【實施方式】 實施例詳 細說：下本發明的〜作，— 請參照圖1所示， 其為本發，卩溶全吸收珍 珠粉的製造 • 1300336 方法，其包含下列步驟： 第一階段的鮮乳浸泡程序（步驟si ίο)，於珍珠原料1101 •重500公克（g、Grams)以水徹底洗淨，去除表面的沾覆物及 ‘ 雜質後瀝乾，加入鮮乳（Milk)1102重500公克（g、Grams)浸 泡1日後’將其濾乾生成第一珍珠生成物110 3 ’進入至弟一 階段。其中，含珍珠成分的原料1101可為天然珍珠、人工養 殖珍珠或珍珠層質；以鮮乳1102浸泡該含珍珠成分的原料 1101可為1到2日。 第二階段的丑腐（Tofu、Bean curd)和綠豆（Green bean) 蒸煮程序（步驟S120)，於第一珍珠生成物1103中加入豆腐 (Tofu、Bean curd)2101 重 125 公克（g、Grams)和綠豆（Green bean)2102重125公克（g、Grams)，均勻混合後加熱蒸煮， 溫度攝氏105〜120度，蒸煮2小時，隨後以逆滲透水（R〇 Water)2103洗淨珍珠並風乾後生成第二珍珠生成物2104,進 入至第三階段。 第三階段的細碎化程序（步驟S130)，將第二珍珠生成物 2104 放入粉碎機(Crusher)3101 與球磨機（Ball mill) 3102 細 碎成100網目（Mesh)細碎珍珠粉末生成物3103，進入至第四 階段。 第四階段的酵素分解程序（步驟S140)，將細碎珍珠生成 物3103加入純水（？1〇^¥&16〇4101調成30重量百分比（_·%) /谷液後’加入0.1重I百分比（wt·%)蛋白水解酵素（pr〇tein ；1300336 ' -1300336 v IX. Description of the invention: [Technical field to which the invention pertains] The present invention relates to a method for producing a fully soluble pearl powder, and more particularly to an active ingredient having a complete retention of the original pearl powder during the manufacturing process and shortening The manufacturing method of pearl powder in the process time. [Prior Art] Pearl powder has been experimentally analyzed to be rich in various amino acids (such as lysine, tryptophan, phenylalanine, methionine, threonine, leucine, isoleucine, and samarium ammonia). Acids, etc., trace elements (such as zinc Zn, selenium Se, magnesium Mg, 锗Ge, etc.), organic bio-calcium and small-molecule polypeptides; in the Compendium of Materia Medica, "[Scent] pearl powder, salty, Cold, non-toxic; (indications) town heart, peace of mind, smashing sputum, pain relief, treatment of annoying heat and thirst, prevention of spermatorrhea white turbidity, the main dystocia of the dead fetus, refreshing color, skin reversal, go to the barrier It is a sacred product of the Chinese people. In the prior art, pearl powder is prepared by steaming and tofu after the traditional method, and then obtaining a fine powder by the water flying method. Today, many people still use this method to prepare pearl powder, or to improve the grinding method or technical method, and dry the pearl powder in a vacuum manner, but such pearl powder has limited solubility and cannot be completely absorbed by the human body. In 1991, Wang Siming and others proposed the "new preparation method of water-soluble whole-component pearl powder series" (Chinese Patent Application No. 90109538.9), which was prepared by first hydrolyzing and separating pearl composite 1300336 with a composite carboxylic acid (including propionic acid, acetic acid, etc.). Carboxylate solution and pearl hard protein, and then hydrolyzed pearl hard protein with 6 volumes, koji and enzyme; mixed with "slow acid acid = 60 degrees Celsius vacuum concentrated in the evaporator to dry. w liquid and The prior art has unavoidable shortcomings, which causes the human to hydrolyze the pearl powder, and treats the pearl hard protein with hydrochloric acid. The amino acid in the sputum is destroyed, and because of its high temperature concentration and drying, and the use of ancient, curved, and Wen ^, and Ming Dynasty rectified the Lin Cai towel office ^ pearl powder as a tool for cold, non-toxic characteristics. [Invention] This is the case, in order to solve the above problems, the present invention provides a kind of instant = pearl The method for producing a powder, the method for producing the powder, completely retains the active ingredient of the original pearl enamel and shortens the processing time. The method for producing the instant absorbing pearl powder disclosed in the present invention , using the P section to carry out Wei dip _ sequence, bean silk mung bean steaming sequence, finely divided procedures, enzyme decomposition procedures, solid matter hydrolysis (four) and vacuum; East knot drying process. ... the first stage of the fresh milk soaking procedure Fresh milk is added to the pearl raw material, and the fresh milk and the pearl raw material are soaked - after a period of time, the first pearl product is formed and enters the second stage. The second stage of the tofu and mung bean cooking process, (4) - the pearl product is added with tofu and The mung bean is uniformly mixed and heated to produce a second pearl product, which enters the third stage. • 1300336 The third stage of the finely divided procedure, the second pearl product is placed in a pulverizer and a ball mill to be finely divided into finely divided pearl powder. The substance enters the fourth stage. In the fourth stage of the enzyme decomposition process, pure water, proteolytic enzyme and N-terminal peptide decomposing enzyme are mixed and reacted to form a pearl amino acid product. , pearl peptide product, trace compound and precipitated solid matter. The fifth stage solid matter hydrolysis procedure is used to add the precipitated solid matter to human; === dry; organic Bio-Liquid: Pearl peptide production 'Trace element mixing: ==Products, the beneficial effect of the present invention is == the substance is dissolved total absorption pearl powder. Method, the instant reading is full of powder (4) ^ dissolved full seam The order of the production of pearl powder, the manufacturing process of pearl powder, the method of improving the production of pearl powder, the non-destructive pearl powder has a nutritional activity of eight short, no strong acid treatment such as hydrochloric acid, and the Venai grade has an easy-to-print ratio of 77. The pearl powder particles can not be burdened by the environment. It is a green and environmentally-friendly product, etc. /, the process of the machine, reducing the manufacturing man-hours and [implementation] The details of the examples: The following is a description of the present invention, which is shown in Fig. 1, which is a method for producing a lysium-solubilized pearl powder. The 1300336 method comprises the following steps: a first stage fresh milk soaking procedure (step si ίο) In the pearl material 1101 • Weigh 500g (g, Grams) thoroughly washed with water, remove the surface of the stain and 'impurities, drain, add fresh milk (Milk) 1102 weight 500 grams (g, Grams) soak 1 In the future, 'filter it out to produce the first pearl. The adult 110 3 ’ enters the stage of the younger brother. The raw material 1101 containing the pearl component may be a natural pearl, an artificially cultivated pearl or a nacre; the raw material 1101 in which the pearl component is soaked in the fresh milk 1102 may be 1 to 2 days. The second stage of the ugly (Tofu, Bean curd) and the green bean (green bean) cooking process (step S120), adding tofu (Bean curd) 2101 to the first pearl product 1103 weighs 125 grams (g, Grams) And Green bean 2102 weighs 125 grams (g, Grams), uniformly mixed, heated and cooked, the temperature is 105 ~ 120 degrees Celsius, cooking for 2 hours, then washed with reverse osmosis water (R〇Water) 2103 pearls and air dried The second pearl product 2104 is generated and proceeds to the third stage. In the third stage of the pulverization process (step S130), the second pearl product 2104 is placed in a pulverizer 3101 and a ball mill 3102, and is pulverized into 100 mesh (Mesh) finely divided pearl powder product 3103. The fourth stage. In the fourth stage enzyme decomposition procedure (step S140), the finely divided pearl product 3103 is added to pure water (?1〇^¥&16〇4101 is adjusted to 30 weight percent (_·%) / valley liquid after adding '0.1 weight I percentage (wt·%) proteolytic enzyme (pr〇tein; 1300336 ' -
Hydrolase) 4102與〇· 01重量百分比（wt.%)N端胜肽分解酶 (N-terminalprotease)4103充份混合進行反應，反應溫度攝氏 、 35度，反應時間1.5小時，生成珍珠胺基酸生成物4104、珍珠 • 胜肽生成物4105、微量元素混合物4106與沉澱固形物4107。 其中，蛋白水解酵素（Protein Hydrolase) 4102之使用添加 量可為0· 1到1重量百分比(wt·%); N端胜肽分解酶（N-terminal proteas e)4103之使用添加量可為〇·〇1到0· 1重量百分比（wt. %);反應溫度可為攝氏30度到50度；反應時間可為1到3小時。 第五階段的固形物水解程序（步驟S150)，將沉澱固形物 4107中加入食用酸5101乳酸（Lactic Acid)重500公克（g、 Grams)，再加入20倍的純水（Pure Water)5102，充分混合進 行反應，反應時間1.5小時，反應溫度攝氏30度，生成珍珠有 機生物鈣水溶液5103。其中，食用酸510 1也可為醋酸（Acetic Acid)' 擰檬酸（Citric Acid)、蘋果酸（Malic Acid)、葡萄糖 酸（Gluconic Acid)、抗壞血酸（Ascorbic acid)或填酸 (Phosphoric Acid、Or thophosph oric Ac id)等；固形物水解 程序其反應時間可為1至3小時。 第六階段的真空凍結乾燥程序程序（步驟S160)，將珍珠 胺基酸生成物4104、珍珠胜肽生成物4105、微量元素混合物 4106與珍珠有機生物鈣水溶液5103以真空凍結乾燥機 (Freeze Dryer)6101處理，以溫度攝氏零下50度凍結後，再 經真空乾燥處理48小時，乾燥後的各項產物混合均勻，並以 M300336 求磨機（Ball miii) 61〇2研磨成細粉，並經殺菌後製成最終 產物即溶全吸收珍_關。其巾，真心綠乾燥機加咖Hydrolase) 4102 is mixed with 〇·01 weight percent (wt.%) N-terminal protease 4103 in a reaction mixture. The reaction temperature is Celsius, 35 degrees, and the reaction time is 1.5 hours to form a pearl amino acid. 4104, pearl • peptide product 4105, trace element mixture 4106 and precipitated solid 4107. Among them, Protein Hydrolase 4102 can be added in an amount of 0.1 to 1 weight percent (wt·%); N-terminal proteas e 4103 can be added in an amount of 〇 • 〇1 to 0·1 weight percent (wt. %); reaction temperature may be 30 degrees Celsius to 50 degrees Celsius; reaction time may be 1 to 3 hours. In the fifth stage solid matter hydrolysis procedure (step S150), the precipitated solid matter 4107 is added with 500 mg of lactic acid 5101 lactic acid (g, Grams), and 20 times of pure water (Pure Water) 5102 is added. The reaction was thoroughly mixed, the reaction time was 1.5 hours, and the reaction temperature was 30 degrees Celsius to form a pearl organic biological calcium aqueous solution 5103. Among them, the edible acid 510 1 may also be Acetic Acid's Citric Acid, Malic Acid, Gluconic Acid, Ascorbic Acid or Phosphoric Acid, Or. Thophosph oric Ac id); the solids hydrolysis procedure can be carried out for a period of from 1 to 3 hours. The sixth stage vacuum freeze drying program (step S160), the pearl amino acid product 4104, the pearl peptide product 4105, the trace element mixture 4106 and the pearl organic biological calcium aqueous solution 5103 as a vacuum freeze dryer (Freeze Dryer) 6101 treatment, after freezing at a temperature of minus 50 degrees Celsius, and then drying under vacuum for 48 hours, the dried products are uniformly mixed, and ground to a fine powder by M300336 grinding machine (Ball miii) 61〇2, and sterilized. After the final product is dissolved, it is fully absorbed. Its towel, true green dryer and coffee
Dryer)61G1的操作溫度可為攝氏零下跑8〇度、操作時間可 為24至72小時。 本發明即溶全吸收珍珠粉的製造方法所製成之最级產 物即溶全吸收珍珠粉剛，具有可完全轉於水中及易被消 ^及收的特性，即溶全吸收天然珍珠粉61_溶解度測試程 序如下.取2公克（g、Grams)的即溶全吸收珍珠 鳩升⑽預定溫度的純水㈣中 二:轉上:::拌90秒’再用轉速每分鐘3_轉》ΡΜ)離 〜7刀鐘後取出沉澱物烘乾3〇分鐘，再依 始重量）Χ1〇〇%的公式，計算數據如下： 里差）+(原 測試狀態 水溫18 °C以下 表溫 18The Dryer) 61G1 can operate at 8 degrees Celsius and 24 to 72 hours. The most advanced product prepared by the method for producing the instant total absorption pearl powder of the present invention is a fully absorbed pearl powder, which has the characteristics of being completely converted into water and easily removed and collected, that is, the fully absorbed natural pearl powder 61 _ Solubility test procedure is as follows. Take 2 grams (g, Grams) of the instant total absorption pearl 鸠 (10) the pure temperature of the predetermined temperature (four) in the second: turn::: mix 90 seconds 'reuse speed 3 _ turn per minute" ΡΜ) After removing the precipitate from ~7 knives and drying for 3 minutes, and then according to the formula of the initial weight) Χ1〇〇%, the calculation data is as follows: 里差)+ (the original test state water temperature below 18 °C table temperature 18
To? 25 °C^40^°C 40 °c1Tl 造方法所製成之最終產物即溶全合王吸收珍珠粉的 提升珍珠粉的溶解度，進而使珍珠粉=:二確能有 見圖2所示，為本發明所製成之即、、☆八 _有攻吸收； / 合王吸收天然珍珠粉6 1300336 * ' 之電子顯微鏡檢測之圖像，可知本發明所製成之即溶全吸收 天然珍珠粉6103確可達到奈米等級之粉末。 雖然本發明以前述之較佳實施例揭露如上，然其並非用 以限定本發明，任何熟習相像技藝者，在不脫離本發明之精 神和範圍内，所作更動與潤飾之等效替換，仍為本發明之專 利保護範圍内。 【圖式簡單說明】 圖1為本發明即溶全吸收珍珠粉的製造方法之流程圖；以 及 圖2 為本發明所製備之即溶全吸收天然珍珠粉之電子顯 微鏡檢測之圖像。 【主要元件符號說明】 步驟S110 鮮乳浸泡程序 步驟S120 豆腐和綠豆蒸煮程序 步驟S130 細碎化程序 步驟S140 酵素分解程序 步驟S150 固形物水解程序 步驟S160 真空凍結乾燥程序 1101 珍珠原料 1102 鮮乳 1103 第一珍珠生成物 2101 豆腐 12 .1300336 2102 綠豆 2104 第二珍珠生成物 3101 粉碎機 3102 球磨機 3103 細碎珍珠粉末生成物 4101 純水 4102 蛋白水解酵素 4103 N端胜肽分解酶 4104 珍珠胺基酸生成物 4105 珍珠胜肽生成物 4106 微量元素混合物 4107 沉澱固形物 5101 食用酸 5102 純水 5103 珍珠有機生物妈水溶液 6101 真空凍結乾燥機 6102 球磨機 6103 即溶全吸收珍珠粉 13To? 25 °C^40^°C 40 °c1Tl The final product made by the method is the solubility of the pearl powder which is absorbed by the absorbing pearl powder, so that the pearl powder =: It is shown that the invention is made, that is, ☆ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ Pearl powder 6103 can indeed reach the nano-grade powder. While the present invention has been described above in terms of the preferred embodiments thereof, it is not intended to limit the invention, and the equivalent of the modification and retouching of the present invention is still within the spirit and scope of the present invention. Within the scope of patent protection of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a flow chart showing a method for producing a fully soluble pearl powder of the present invention; and Fig. 2 is an image of an electron microscopic mirror for the instant total absorption natural pearl powder prepared by the present invention. [Main component symbol description] Step S110 Fresh milk soaking procedure Step S120 Tofu and mung bean cooking procedure Step S130 Fragmentation procedure Step S140 Enzyme decomposition procedure Step S150 Solid matter hydrolysis procedure Step S160 Vacuum freeze drying procedure 1101 Pearl raw material 1102 Fresh milk 1103 First Pearl product 2101 Tofu 12.1300336 2102 Mung bean 2104 Second pearl product 3101 Crusher 3102 Ball mill 3103 Finely divided pearl powder product 4101 Pure water 4102 Proteolytic enzyme 4103 N-terminal peptide decomposition enzyme 4104 Pearl amino acid product 4105 Pearl Peptide Production 4106 Trace Element Mixture 4107 Precipitate Solids 5101 Edible Acid 5102 Pure Water 5103 Pearl Organic Biology Aqueous Solution 6101 Vacuum Freeze Dryer 6102 Ball Mill 6103 Instant Dissolved Pearl Powder 13