TW593677B - Bio-chip-type flow cytometer (FCM) bio-detector with fiber optical type barcode carrier chip - Google Patents

Abstract

A bio-chip-type flow cytometer (FCM) bio-detector with a fiber optical type barcode carrier chip mainly includes a bio particle (e.g. protein, antibody, bacteria, or deoxyribonucleic acid (DNA)) added on an optical fiber carrier installed with a barcode. The optical fiber carrier containing a bio particle is injected into a chip-type FCM according to the invention. The carrier is delivered to a detector in a cellular detection device hydraulically or by a suction force. The detector senses the barcode on the carrier for identifying the type, amount and other bio parameters of the bio particle assayed by the FCM.

Description

593677 五、發明說明（1) 發明領域： 本發明係有關於一種光纖式條碼載體晶片之生物晶片 式流式細胞儀生物體檢測裝置.，其主要係在本發明之晶片 式流式細胞儀分析生物粒子前，於生物粒子加上一辨識條 碼，並在晶片式流式細胞儀分析生物粒子過程中，即時將 分析結果與生物粒子屬性做連結，以得到生物粒子的類別 、數目及其他生物參數。 發明背景： 在眾多細胞檢測裝置中，流式細胞儀是現代生物科技 中（如第1圖所示）最為普遍使用的分析儀器之一，流式 __ 細胞儀的應用範圍相當廣泛，如可分析基因表現型（ phenotypic analysis)、去氧核糖核酸分析（DNA analysis)、染色體的分析與分類(chromosome analysis and s o r t i n g)、細胞動力學分析、核糖核酸分析（R N A analysis)及可做其他細胞功能上的研究（functional s t ud i e s)分析，並可讓生物粒子黏附不同顏色的螢光劑 作細胞或生物的物理、化學上之定性與定量，但因螢光劑 顏色有限（&lt; 1 0種），故最多只能同時檢查1 0種，無法做大 量檢驗，且無法對生物晶片做檢驗，如何同時檢驗並做大 量的分析是大家所引頸期盼的。 Ο 發明目的與概述： 本發明之主要目的，係藉使用光纖式條碼載體晶片之 生物晶片式流式細胞儀生物體檢測裝置，改善傳統流式細 胞儀的分析大量樣品之效率。593677 V. Description of the invention (1) Field of the invention: The present invention relates to a biological wafer type flow cytometer biological detection device for an optical fiber type barcode carrier wafer, which is mainly based on the analysis of the wafer type flow cytometer of the present invention. Before the biological particle, add an identification barcode to the biological particle, and in the process of analyzing the biological particle by the wafer flow cytometer, link the analysis result with the biological particle attribute in real time to obtain the type, number and other biological parameters of the biological particle . Background of the invention: Among many cell detection devices, flow cytometry is one of the most commonly used analytical instruments in modern biotechnology (as shown in Figure 1). The application range of flow cytometer is quite wide. Analysis of phenotypic analysis, DNA analysis, chromosome analysis and sorting, cell dynamics analysis, RNA analysis and other cellular functions Functional st ud ies analysis, and can allow biological particles to adhere to different colors of fluorescent agents for the physical and chemical qualitative and quantitative of cells or organisms, but due to the limited color of fluorescent agents (<10 species) Therefore, at most 10 types can be inspected at the same time, a large number of inspections cannot be performed, and biochips cannot be inspected. How to simultaneously inspect and perform a large number of analyses is what everyone expects. 〇 Purpose and summary of the invention: The main purpose of the present invention is to improve the efficiency of analyzing a large number of samples by a conventional flow cytometer by using a biological wafer type flow cytometer biological detection device using an optical fiber type barcode carrier wafer.

第4頁 593677 五、發明說明（2) 本發明之另一目的，藉使用光纖式條碼載體晶片之生 物晶片式流式細胞儀生物體檢測裝置，改善傳統流式細胞 儀分析資料的正確性。 本發明之又一目的，係藉使用光纖式條碼載體晶片之 生物晶片式流式細胞儀生物體檢測裝置，降低流式細胞儀 的成本。 本發明之又一目的，配合光纖式條碼生物晶片晶片分 選裝置可進一步讓流式細胞儀揀選吾人欲分離的生物體。 本發明之光纖式條碼載體晶片之生物晶片式流式細胞 儀生物體檢測裝置，其係將生物粒子加於光纖載體上，注 入生物晶片式之流式細胞儀中，並設一偵測器藉以讀取光 纖載體上的待測物及光纖載體所刻晝之條碼，而得知晶片 上所載生物粒子的資料及其他生物檢測參數。 為使 貴審查委員對本發明之目的、結構、特徵及功 效有進一步的瞭解，茲舉較佳實施例並配合圖示詳細設說 明如后。 圖號說明： 8液流糸統 1 0樣品 1 2樣品管 _ 1 3勒液管 1 4鞘液 1 6流動室 1 7噴嘴Page 4 593677 V. Description of the invention (2) Another object of the present invention is to improve the accuracy of the analysis data of the conventional flow cytometer by using the biological wafer type flow cytometer biological detection device of the optical fiber type barcode carrier wafer. Another object of the present invention is to reduce the cost of a flow cytometer by using a biological wafer type flow cytometer biological detection device using an optical fiber type barcode carrier wafer. Yet another object of the present invention is to further enable the flow cytometer to select organisms to be separated by the flow cytometer in combination with the optical fiber barcode biochip wafer sorting device. The biological wafer type flow cytometer biological detection device of the optical fiber type barcode carrier wafer of the present invention is a method in which biological particles are added to the optical fiber carrier and injected into the biological wafer type flow cytometer, and a detector is provided to thereby Read the barcode on the optical fiber carrier to be tested and the day code engraved on the optical fiber carrier, and learn the data of biological particles and other biological detection parameters on the wafer. In order to make your reviewers have a better understanding of the purpose, structure, features, and functions of the present invention, the preferred embodiments will be described in detail with reference to the drawings. Description of drawing number: 8 flow system 1 0 sample 1 2 sample tube _ 1 3 liquid tube 1 4 sheath liquid 1 6 flow chamber 1 7 nozzle

第5頁 593677 五、發明說明（3) 器器 置 測測 器 裝 統 檢檢 生板理置 統 系區 角角 發轉處裝 系 測測源射射孔 衝偏號選 測 檢檢光散散小 脈壓信分器檢 柱學學測向向出滴電電片片集碼 液光光檢前側流液充高晶晶收條 89 0 24678024689 11 2 22222333334Page 559677 V. Description of the invention (3) Device installation and measurement equipment Installation inspection and inspection board management system inspection system angle corner rotation and rotation installation system Measurement source perforation Punctuation number selection Inspection inspection Scattering small pulse pressure signal divider, columnology, direction detection, drop electricity film, code collection, liquid light, front side, liquid filling, high crystal, receipt 89 0 24678024689 11 2 22222333334

體液片， 載括晶中 碼包、備 條要9製 式主4品 纖置統樣 光裝系於 Φ-·- 晶光置物 物、裝測 生8理待 之統處品 片系號樣 晶流信將 第6頁 593677 五、發明說明（4) 加於一光纖載 述）， 的資料 氧核糖 殊設計 體或載 (此種 源2 2 2内嵌 樣品管 合，混 合後， 18, 通過光 氬雷射 源2 2 物上， 受到激 向放置 器2 6 晶片信 質（如 份與功 前 藉以同 整合； 核酸、 之載體 體的樣 螢光染 照射會 於鞘液 1 2内 合處即 由流動 液柱1 學檢測 、氦氖 形成的 欲篩選 發而產 濾光片 ，收集 號處理 大小、 能）。 述該待 體晶 時檢 光纖 蛋白 上， 品1 料與 產生 管1 的樣 稱為 室1 8中 區2 雷射 光束 的晶 生螢 及前 螢光 裝置 形狀 片， 驗不 載體 質、 該包 0， 欲篩 螢光 3之 品1 流動 6的 的混 0， )或 照射 片待 光， 向散 信號3 4 、複 該光纖 同大量 晶片主 抗體或 含待測 經由特 選的細 )，放 喷嘴1 0與樣 室1 6 喷嘴1 合液以 檢測光 甚至是 在通過 測物經 檢測光 射角檢 、前向 ，量測 雜度及 載體晶片 生物體以 要係指一 抗原等待 生物體之 殊屬性之 胞待測物 入樣品管 7中，利 品管1 2 ，樣品1 7喷出成 一次一個 源2 2通 半導體雷 光學檢測 由檢測光 源2 0入 測器2 4 散射光及 晶片待測 其他可被 係設有 加速整 種载體 測物可 載體製 螢光染 結合後 1 2中 用水壓 外的鞘 0與鞘 為細胞 細胞或 常是氣 射所構 區2 0 源2 2 射與液 、側向 側向散 物的物 螢光染 條碼（詳後 個檢測流程 ，細胞、去 黏附在此特 成單個生物 料染色後 ，受檢測光 ，樣品管1 力或吸力， 液1 4相混 液1 4相混 或載體液柱 載體的方式 體雷射（如 成’檢測光 之晶片待測 照射後，會 柱垂直的方 散射角檢測 射光，透過 理及化學性 料彳貞測的成 測物與載體間的黏著劑可用生物晶片黏附生Body fluid tablets, containing crystal medium code bags, 9 strips of standard preparation, and 4 main fiber placement samples are mounted on the Φ- ·-crystal light storage, and are placed on the 8th place to be tested. Liuxin will be on page 6 593677 V. Invention description (4) added to a fiber optic description), the oxygen ribose special design body or containing (this source 2 2 2 embedded sample tube, after mixing, 18, passed The light argon laser source 2 2 is subject to the positivity of the chip 2 6 chip (eg, the same integration as before work; the nucleic acid, the carrier-like fluorescent dye will be irradiated on the inner part of the sheath fluid 12 That is, the filter is formed by the liquid column 1 for scientific detection and helium-neon to produce a filter, and the collection number is processed. The size and energy can be collected. It is called the crystallized fluorescent and front fluorescent device shape sheet of the laser beam in the middle area 2 of the chamber 18, the carrier material, the package 0, the product of the fluorescent 3 to be screened, the flow of the mixed 6, or the irradiation. A piece of light to be scattered, a scattered signal 3 4, a complex optical fiber with a large number of wafer main antibodies or (Selected fine), put the nozzle 10 and the sample chamber 16 into the nozzle 1 to combine light to detect the light or even pass the test object through the detection of light angle detection, forward direction, measurement of heterogeneity and carrier wafer organisms to refer to An antigen waits for the special property of the organism to be tested into the sample tube 7, the quality tube 1 2 and the sample 17 are ejected into a source 2 at a time 2 through the semiconductor optical optical detection by the detection light source 2 0 into the detector 2 4 Scattered light and wafer to be tested Others can be equipped with a whole carrier to accelerate the measurement of the object can be combined with fluorescent staining of the carrier 1 2 Water-resistant outer sheath 0 and the sheath is a cell or a region often formed by air emission 2 0 Source 2 2 Fluorescently dye barcodes with liquid and laterally scattered objects (Detailed in the next detection process. Cells and de-adhesion are stained in this special single biological material. After the detection light, the sample tube 1 force Or suction, liquid 1 4 phase mixed liquid 1 4 mixed or carrier liquid column carrier body laser (such as the 'detection of the light to be measured after the wafer to be tested, the vertical scattering angle of the column will detect the light, transmission and chemical properties Adhesive between test object and carrier Biochip attachment

第7頁 593677 五、發明說明（5) 物體之方法，如基因吸附輔助劑（如生物素b i 〇 t i η)，因 黏著劑與基因、抗生物性蛋白（a v i d i η)有強結合力；一 方面，將待測之基因、蛋白質或抗原等染上不同的螢光， 另一方面再將不同之光纖載體黏以不同之待測物，然後將 待測之基因、蛋白質或抗原與光纖載體上的待測物混合雜 交（hybridization)，其相對應之抗體與抗原或基因結合 ，會使光纖載體與螢光結合，洗去不結合之螢光及待測物 ，再注入樣品1 0後流至檢測光源2 2並讀取帶有螢光之 光纖載體，則可判別得知待測物的定量資料，即有螢光者 為待測物。 φ 再者，該光學檢測區2 0下方、流出小孔2 7之前係 設有一條碼檢測糸統4 9 ^其包括條碼檢測光源5 0及條 碼檢測器5 2 (如快速A v a 1 a n c h d e t e c t 〇 r )，此時因條碼 檢測光源5 0不激發待測物上的螢光，又因蛋白質、去氧 核糖核酸、細胞等生物體及光纖為透明，而周圍之液體其 折射率與光纖之折射率不同，可產生條狀影像（其粗細不 同代表0或1之訊號），故可以清楚讀取光纖上條碼信號， 得知條碼所記錄的資訊，即知該光纖所帶生物體為何物， 因條碼數目種類可大量至數十萬種，故此法可檢查數十萬 種生物體，且進一步檢查已篩選出有螢光之生物體，因此❼ 只需要讀取光學檢測系統1 9檢測到的帶有螢光之光纖載 體，故條碼檢測器5 2不需每個光纖載體都辨識，因條碼 檢測系統4 9選擇性讀取每一待測物，可節省讀取條碼暫 存記憶體之容量，而光纖載體長度加長可使樣品液體流動Page 593677 V. Description of the invention (5) Object methods, such as gene adsorption aids (such as biotin bi 〇ti η), because of the strong binding force between the adhesive and genes, avidin (avidi η); , Stain the genes, proteins, or antigens to be tested with different fluorescence, on the other hand, stick different fiber carriers to different test objects, and then combine the genes, proteins, or antigens to be tested with the fiber carriers. Hybridization of the test object. The corresponding antibody binds to the antigen or gene, which will bind the optical fiber carrier to the fluorescent light. The unbound fluorescent light and the test object are washed away, and then injected into the sample and flowed to the test. The light source 22 and the optical fiber carrier with fluorescent light are read, and then the quantitative data of the object to be measured can be determined, that is, the person with fluorescence is the object to be measured. φ In addition, a code detection system 4 9 is provided below the optical detection area 20 and before the outflow aperture 2 7 ^ It includes a bar code detection light source 50 and a bar code detector 5 2 (such as fast Ava 1 anchdetect 〇r ), At this time, because the bar code detection light source 50 does not excite the fluorescent light on the test object, and because proteins, DNA, cells and other organisms and the optical fiber are transparent, the refractive index of the surrounding liquid and the refractive index of the optical fiber Different, can produce a bar image (the thickness of which represents a 0 or 1 signal), so you can clearly read the bar code signal on the optical fiber, and learn the information recorded by the bar code, that is, what the organism carried by the optical fiber is, because of the bar code The number of species can be as large as hundreds of thousands, so this method can inspect hundreds of thousands of organisms, and further inspection has been screened out for fluorescent organisms, so ❼ only need to read the bands detected by the optical detection system 19 Fluorescent optical fiber carrier, so the barcode detector 5 2 does not need to be identified by each optical fiber carrier. Because the barcode detection system 4 9 selectively reads each object to be tested, it can save the capacity of the temporary memory for reading the barcode, and optical fiber Extended the length of the sample liquid can flow

593677 五、發明說明（6) 時，光纖載體與液體流動方向平行而利於檢測器讀取其橫 刻之條碼，但光纖載體的長度須與雷諾數的公式 (Reynolds num-ber e q u a t i ο η )相酉己合，故液柱流道之大小 應比光纖載體之直徑略大，但不可大於光纖載體之長度， 否則光纖載體將於液柱流道中亂轉增加條碼讀取時的困難 ，且此時液柱液道之流裡可由管長度、寬度與外加注入力 ，控制使有足夠時間由偵測器瞬時記憶到記憶體中，以便 事後分析。593677 5. In the description of the invention (6), the optical fiber carrier is parallel to the liquid flow direction, which is convenient for the detector to read the horizontal barcode, but the length of the optical fiber carrier must be in accordance with the formula of Reynolds num-ber equati ο η The size of the liquid column flow channel should be slightly larger than the diameter of the fiber carrier, but it should not be larger than the length of the fiber carrier. Otherwise, the fiber carrier will run around the liquid column channel to increase the difficulty in reading the barcode. The flow of the liquid column and the liquid channel can be controlled by the length, width and external injection force of the tube so that there is enough time to be memorized by the detector into the memory for analysis afterwards.

請參閱第3圖及第4圖，光纖載體的構造為直徑極細 、約125微米（// m)的纖維，利用紫外線雷射6 0 (如KrF 或Ar準分子雷射）透過條碼光柵光罩6 2 ，在光纖6 4上 刻以粗細不同之條碼，光纖6 4進一步切割成長度在2 0 0 /z m之内的一小段光纖，每小段光纖可如此編碼（encode )，以代表不同之細胞、去氧核糖核酸、蛋白質、抗體或 抗原等生物體。 請再參閱第2圖，為了獲得更多的資訊，另設有一細 胞分選器3 6 ，可將細胞依篩選標準做分類，在流動室1 鄱 6的喷嘴1 7喷出成為細胞或載體的液柱1 8時，液柱1 8繼續往下滴落脫離光學檢測區2 0及條碼檢測系統4 9 ，液柱1 8會斷裂成一連串均勻的液滴2 8 ，此液滴2 8 形成的頻率訊號約為30kHz，此訊號亦會讓流動室1 6產 生相同頻率的機械振動，於是液柱1 8可連續斷裂成一連 串均勻的液滴2 8 ，液滴2 8形成的速度大約是每秒2 0 0 0 個以下，因此每1 5個液滴中，才有一個液滴内包含晶片載Please refer to Figures 3 and 4. The structure of the fiber carrier is a fiber with an extremely small diameter of about 125 micrometers (/ m), which uses a UV laser 60 (such as KrF or Ar excimer laser) to pass through the barcode grating mask. 62, engraved with different thickness bar codes on the optical fiber 64, the optical fiber 64 is further cut into a small piece of optical fiber with a length within 200 / zm, and each small piece of optical fiber can be encoded in this way to represent different cells , DNA, proteins, antibodies or antigens. Please refer to Figure 2 again. In order to obtain more information, there is another cell sorter 3 6, which can sort the cells according to the screening criteria. The nozzles 17 in the flow chamber 1 鄱 6 are ejected into cells or carriers. When the liquid column 18, the liquid column 18 continues to drop off the optical detection area 20 and the barcode detection system 4 9. The liquid column 18 will break into a series of uniform liquid droplets 2 8. The frequency signal is about 30kHz. This signal will also cause the flow cell 16 to generate mechanical vibrations of the same frequency. Therefore, the liquid column 18 can be continuously broken into a series of uniform droplets 2 8, and the formation speed of the droplets 2 8 is about 1 second. Less than 2 0 0, so only 1 in 15 droplets contains a wafer carrier

593677 五、發明說明（7) 體，而且晶片的性質是在進入液滴前就已被測定了 ，若該 晶片的條碼與所欲關心的晶片特性相符，則此生物晶片式 流式細胞儀在此晶片形成液滴時，位於光學檢測區下万的 充電脈衝發生器3 0 ，就對整個液柱1 8充以指定的電荷 ，如此一來，被選定的片形成液滴時就帶有特定的電荷， 未被選定的晶片則不帶有任何電荷，帶有電荷的液滴落下 時，經過一高電壓偏轉板3 2時，則依同性電相斥異性電 相吸的原理’洛入指定的晶片分選裝置3 6内5完成晶片 分類的收集。 在晶片分選裝置3 6中排列有數個小收集器3 8 ，如 __ 第2圖下方所示，此成排之小收集器3 8在一自動控制車 軌之上，由電腦控制其移動，當光學檢測裝置測得其訊號 為A時，則測定訊號傳至電腦，並移動收集器A至加電為 正或負電偏轉板3 2之下收集該粒子，但因光學檢測裝置 測到之時間至小收集器3 8收集到之時間間隔極短，故需 將落下的路徑加長，或可設置複數排的小收集器3 8同時 操作，以縮短路徑長度。 因此，本發明的優點係藉使用光纖載體晶片及條碼讀 取裝置，改進習知流式細胞儀的只能同時分析小於1 〇種生 物體之缺點，本發明可同時測出數萬種生物體之數目，故 Φ 可增加流式細胞儀之效率與降低篩選成本；另一優點則為 配合晶片分選裝置，可進一步揀選分裝吾人欲分離的生物 體。 綜上所述，雖然本發明已以較佳實施例揭露如上，然593677 V. Description of the invention (7) body, and the properties of the wafer are measured before entering the droplet. If the barcode of the wafer is consistent with the characteristics of the wafer of interest, the biological wafer flow cytometer is When this wafer forms droplets, the charging pulse generator 30 located below the optical detection area charges the entire liquid column 18 with a specified charge. In this way, the selected sheet is formed with a specific droplet. The unselected wafer does not have any charge. When the charged droplets fall through a high-voltage deflection plate 32, the principle of the same-phase electric repulsion and the opposite-phase electric phase attraction is entered. The wafer sorting device 3 within 6 completes the collection of wafer sorting. There are several small collectors 3 8 arranged in the wafer sorting device 36, as shown in the bottom of Figure 2. This row of small collectors 3 8 is on an automatic control rail, and its movement is controlled by a computer. When the signal is measured by the optical detection device as A, the measurement signal is transmitted to the computer, and the collector A is moved to collect the particles under the positive or negative deflection plate 32 when the power is turned on, but it is detected by the optical detection device. The time interval collected by the time-to-small collector 38 is extremely short, so the falling path needs to be lengthened, or a plurality of rows of small collectors 38 can be set to operate at the same time to shorten the path length. Therefore, the advantage of the present invention is that by using a fiber-optic carrier wafer and a bar code reading device, the conventional flow cytometer can be used to analyze the disadvantages of less than 10 organisms at the same time. The present invention can measure tens of thousands of organisms at the same time. Therefore, Φ can increase the efficiency of the flow cytometer and reduce the cost of screening. Another advantage is that it can be used with the wafer sorting device to further sort the organisms that we want to separate. In summary, although the present invention has been disclosed as above with the preferred embodiment,

第10頁 593677 五、發明說明（8) 其並非用以限定本發明，任何熟習此技藝者，在不脫離本 發明之精神和範圍内，當可作各種之更動與潤飾，因此本 發明之保護筢圍當視後附之申請專利範圍所界定者為準。Page 10 593677 V. Description of the invention (8) It is not intended to limit the invention. Any person skilled in the art can make various modifications and retouches without departing from the spirit and scope of the invention. Therefore, the invention is protected.筢 Wai Dang shall be determined as defined in the scope of the attached patent application.

第11頁 593677 圖式簡單說明 第1圖：係傳統流式細胞儀結構示意圖。 第2圖：係本發明光纖式條碼載體晶片之生物晶片式流式 細胞儀生物體檢測裝置示意圖。 第3圖：係本發明之光纖條碼生物晶片製作示意圖。 第4圖：係本發明之光纖條碼生物晶片示意圖。Page 11 593677 Schematic description of the diagram Figure 1: Schematic diagram of the traditional flow cytometer. FIG. 2 is a schematic diagram of a biological wafer type flow cytometer biological detection device of the optical fiber type barcode carrier wafer of the present invention. FIG. 3 is a schematic diagram of manufacturing an optical fiber barcode biochip according to the present invention. FIG. 4 is a schematic diagram of the optical fiber barcode biochip of the present invention.

II

第12頁Page 12

Claims (1)

593677 六、申請專利範圍 1 · 一種光纖式條碼載體晶片之生物晶片式流式細胞儀生 物體檢測裝置，用以進行生物粒子之偵測，其主要係 包含： 一待測物，該待測物與螢光染料結合； 一光纖條碼載體晶片，用以承載待測物； 一流道液流系統，將該待測物形成可供分析的適當流 體狀態； 一光學檢測系統，位於該流道液流系統下方，利用光 學原理檢測該待測物的生物參數； 一光纖條碼讀取裝置，設於該光學檢測區之下方，讀 _丨 取該光纖條碼載體晶片上相關資訊；以及， 一晶片信號處理裝置，連接控制該流道液流系統、該 光學檢測系統、該光纖條碼晶片讀取裝置與該晶片 分選裝置。 2 ·如申請專利範圍第1項所述之光纖式條碼載體晶片之 生物晶片式流式細胞儀生物體檢測裝置，其中該待測 物與螢光染料結合的方式，可採用生物晶片黏附生物 體的方式。 3 ·如申請專利範圍第1項所述之光纖式條碼載體晶片之 生物晶片式流式細胞儀生物體檢測裝置，其中該光纖 # 條碼載體晶片之直徑為微米等級。 4 ·如申請專利範圍第1項所述之光纖式條碼載體晶片之 生物晶片式流式細胞儀生物體檢測裝置，其中該光纖 條碼載體之長度為微米等級。593677 VI. Scope of patent application1. A biological wafer type flow cytometer biological detection device for optical fiber type barcode carrier wafers for detecting biological particles, which mainly includes: a test object, the test object Combined with fluorescent dye; a fiber-optic barcode carrier wafer to carry the object to be measured; first-rate liquid flow system to form the object to a suitable fluid state for analysis; an optical detection system located in the flow channel liquid flow Under the system, the biological parameters of the object to be measured are detected using optical principles; an optical fiber barcode reading device is arranged below the optical detection area, and reads and retrieves relevant information on the optical fiber barcode carrier chip; and, a chip signal processing The device is connected to control the flow channel liquid flow system, the optical detection system, the optical fiber barcode wafer reading device and the wafer sorting device. 2 · The biological wafer type flow cytometer biological detection device of the optical fiber type barcode carrier wafer described in item 1 of the scope of the patent application, wherein the way in which the test object is combined with the fluorescent dye can use a biological wafer to adhere to the biological body The way. 3. The biological wafer type flow cytometer biological detection device of the optical fiber type barcode carrier wafer as described in item 1 of the scope of the patent application, wherein the diameter of the optical fiber # barcode carrier wafer is in the micrometer order. 4 · The biological wafer type flow cytometer biological detection device of the optical fiber type barcode carrier wafer described in item 1 of the scope of the patent application, wherein the length of the optical fiber barcode carrier is in the order of micrometers. 第13頁 593677 六、申請專利範圍 5 ·如申請專 生物晶片 條碼載體 6 ·如申請專 生物晶片 條碼載體 7 ·如申請專 生物晶片 條碼讀取 8 ·如申請專 生物晶片 條碼讀取 置的該待 9 ·如申請專 生物晶片 晶片分選 待測物作 I 0 ·如申請專 生物晶片 分選裝置 裝置設於 II ·如申請專 生物晶片 裝置由電 利範圍 式流式 晶片上. 利範圍 式流式 上的粗 利範圍 式流式 裝置包 利範圍 式流式 裝置選 測物。 利範圍 式流式 裝置， 適當的 利範圍 式流式 中設有 一自動 利範圍 式流式 腦控制 第1項 細胞儀 刻有粗 第5項 細胞儀 細條碼 第1項 細胞儀 含條碼 第5項 細胞儀 擇性讀 第1項 細胞儀 位於該 揀選。 第9項 細胞儀 數個並 控制車 第10項 細胞儀 移動， 所述之光 生物體檢 細不同的 所述之光 生物體檢 ，可由紫 所述之光 生物體檢 檢測光源 所述之光 生物體檢 取每一經 纖式條 剛裝置 條螞。 纖式條 剛骏置 外線雷 纖式條 測裝置 及條媽 纖式條 測裝置 過該光 碼載體晶片之 ’其中該光纖 碼載體晶片之 ’其中該光纖 射刻劃產生。 碼載體晶片之 ’其中該光纖 檢測器。 碼載體晶片之 ’其中該光纖 纖條碼讀取裝 Ο 所述之光纖式條碼載體晶片之 生物體檢測裝置，更可包括一 光纖條碼讀取裝置下方，將該 所述之光纖式條碼載體晶片之 生物體檢測裝置，其中該晶片 排之複數個收集裝置，該收集 軌上。 所述之光纖式條碼載體晶片之 生物體檢測裝置，其中該收集 5亥收集裝置依檢測結果移動至 第14頁 593677 六、申請專利範圍 相對應之偏轉板下方，、以收集該待測物 IBB 第15頁Page 13 593677 VI. Application for patent scope 5 · If applying for a special bio-wafer barcode carrier 6 · If applying for a special bio-wafer barcode carrier 7 · If applying for a special bio-wafer barcode reading 8 · If applying for special bio-wafer barcode reading Wait 9 · If apply for bio-chip wafer sorting to be tested as I 0 · If apply for special bio-wafer sorting device device set at II · If apply for special bio-wafer device by electric range flow chip. Profit range type The coarse range flow device on the flow type includes the selection object of the profit range flow device. The right range flow device, an appropriate range is equipped with an automatic range flow brain control. The 1st cytometer is engraved with a thick 5th cytometer and a thin barcode. The 1st cytometer contains a 5th barcode. Cytometer Selective Reading Item 1 The cytometer is located at this pick. The number of cytometers of the 9th cytometer and the control of the movement of the 10th cytometer of the car, the photobiological examinations with different details of the photobiological examination can be obtained by the photobiological examinations described by the photobiological examination light source in purple Each warp-fiber sliver has a sliver. Fiber strips Gangjun sets outside line fiber strip measurement device and strip fiber fiber strip measurement device ‘of the optical code carrier wafer’ where the optical fiber carrier scribe is generated. The code carrier wafer includes the optical fiber detector. The code carrier wafer, wherein the optical fiber barcode reading device is equipped with the optical fiber type barcode carrier wafer biological detection device, and may further include an optical fiber barcode reading device below the optical fiber barcode carrier wafer. A biological detection device, wherein the wafer is arranged in a plurality of collection devices on the collection rail. The biological detection device of the optical fiber type barcode carrier wafer, wherein the collection device is moved to the 14th page 593677 according to the detection result. VI. Below the deflection plate corresponding to the scope of the patent application to collect the test object IBB Page 15