TW592718B - A composition for reducing hair growth, hair follicle and hair shaft size and hair pigmentation - Google Patents

Abstract

The present invention utilizes natural and/or synthetic serine protease inhibitory agents or botanical extracts containing serine protease inhibitory activity, with or without the addition of one or more isoflavones and/or additional natural extracts containing one or more isoflavones, and their ability to affect changes in mammalian hair growth, hair follicle and hair shaft size and hair pigmentation.

Description

發明範疇 y2月有關種可有效地減緩毛髮生長之方法及組合 。詳°之’本發明有關一種改變毛髮生長速率、縮小毛 囊及=幹尺寸、及減少毛幹色纽著之方法，其係局部施 用含有絲胺酸蛋白酶抑制活性之植物萃取物，尤立是大豆 萃取物諸如豆漿，或前述物質之混合物及調配物，同時結 合以其他活性成分諸如異黃酮。 發明背景 t乳類毛髮之—主要功能係提供外在保護。然而，在 人類身上幾乎已完全喪失此種魏，其毛髮基本上係基於 社父及美觀因素而保持或移除。 已使用許多方法以移除不需要之毛髮，包括剃除、電 針除毛、拔除、雷射及光療纽射治雜抗職素物質。 ，等省用方法不無缺點H _可能於皮膚表面產生 。】痕及刀痕，可能明顯地增加毛髮再生長速率，亦可能留 下不期望產生之短粗毛。雖然電針除毛可長時間使某一區 域保持沒有不㈣之毛髮，但此種方法財是昂貴而疼 =，亦可能導致疤痕。拔除不僅會導致疼痛及不適，亦經 常無法完全移除短毛。使用抗雄性經常產生數種不期望之 =作用，諸如影響肌肉之形成。是故，需要一種可減緩毛 髮生長之較佳方法。 、假性鬚囊炎係為發炎性毛髮疾病，大多發生於鬍鬚區 域。當毛髮尖端穿入皮膚而非穿透毛孔時，則產生發炎性Scope of the invention y2 month related methods and combinations that can effectively reduce hair growth. For details, the present invention relates to a method for changing the rate of hair growth, reducing the size of hair follicles and reducing the size of hair, and reducing the color of hair dryness, which is a topical application of a plant extract containing serine protease inhibitory activity, especially soybean Extracts such as soybean milk, or mixtures and formulations of the foregoing, combined with other active ingredients such as isoflavones. BACKGROUND OF THE INVENTION The main function of dairy hair is to provide external protection. However, such Wei has been almost completely lost in humans, and its hair is basically retained or removed based on social fatherhood and aesthetic factors. Numerous methods have been used to remove unwanted hair, including shaving, acupuncture, hair removal, laser, and phototherapy to treat miscellaneous antibiotic substances. , And other provincial methods are not without disadvantages H _ may be generated on the skin surface. ] Marks and knife marks may significantly increase the rate of hair regrowth, and may also leave short thick hair that is not expected to be produced. Although electro-acupuncture can be used for a long time to keep a certain area free of unsightly hair, this method is expensive and painful, and may also cause scarring. Not only does plucking cause pain and discomfort, it is often not possible to completely remove short hair. The use of anti-males often produces several undesirable effects, such as affecting muscle formation. Therefore, there is a need for a better method of slowing hair growth. Pseudostigmatitis is an inflammatory hair disease, which mostly occurs in the beard area. Inflammatory when the tip of the hair penetrates the skin rather than through the pores

89395A 本紙張尺度適用中國國家標準（CNS)A4規格（210 X 297公釐） (請先閱讀背面之注意事項再填寫1頁) F裝——-ΙΓ訂 • I ϋ n n 1· < 經濟部智慧財產局員工消費合作社印製 592718 < A7 五、發明說明（2 ) -裝—— C請先閱讀背面^%!意事項再填寫本頁} 小囊丘疹。此種過程於黑種人身上極為常見，因其毛髮通 常捲曲，而於銳角下穿出皮膚。密貼式的剃除，尤其是使 用剃刀’易罹患假性鬚囊炎。目前最有效之治療係使該等 毛髮元全於皮膚表面上生長。但經常不期望使用該種治 療。 - 夕毛症係為極為常見之疾病，約有4百分比之女性患 有此症。顏面多毛症經常防礙個人及工作活動，而暫時性 的毛髮移除係為處理多毛患者的主要方式。剃除係為顏面 毛髮最《使用之暫時方法，因為拔除、敷蠟及使用除毛劑 更難以忍受，且需謹慎以避免毛囊炎、色素沉著、及產生 ’危痕通$使用化粧品覆蓋以遮蔽刀痕及短毛，而電針除 毛及熱除可適當地用於永久性除毛。 除毛之取代性或互補式研究係為減緩毛髮生長、縮小 =囊及毛幹尺寸及減少毛幹色素沉著之方法。該項研究可 降低現存毛髮之可見度，使其變得較細較柔軟。與其他除89395A This paper size is in accordance with Chinese National Standard (CNS) A4 (210 X 297 mm) (Please read the precautions on the back before filling in 1 page) F Pack ——- IΓ Order • I ϋ nn 1 · < Ministry of Economic Affairs Printed by the Intellectual Property Bureau's Consumer Cooperatives 592718 < A7 V. Description of Invention (2)-Packing-C Please read the back ^%! This process is very common in black people, because their hair is usually curly and penetrates the skin under acute angles. Adherent shaving, especially when using a razor, is susceptible to false whiskers. Currently the most effective treatment is to grow these hair cells all over the skin surface. However, the use of this treatment is often not expected. -Evening hair is a very common disease, affecting about 4% of women. Facial hirsutism often interferes with personal and work activities, and temporary hair removal is the main way to treat people with hirsutism. Shaving is the most temporary method for facial hair, because removing, applying wax and using a hair remover are more difficult to tolerate, and care must be taken to avoid folliculitis, pigmentation, and the occurrence of 'danger marks'. Use cosmetic cover to cover Knife marks and short hair, while electric needle hair removal and thermal hair removal can be used appropriately for permanent hair removal. Alternative or complementary research on hair removal is a method of slowing hair growth, reducing the size of sac and hair shaft, and reducing pigmentation of hair shaft. The study reduced the visibility of existing hair, making it thinner and softer. And others except

毛=結合時，該方法可促進並延長除毛效果，並降低、除毛 之需求及頻率。長期使用該項研究結果，可使毛髮生長變 經濟部智慧財產局員工消費合作社印製 稀、變軟而降低色素沉著，使其變得較不易察覺，而不需 使用其他除毛方法。 腋部(腋窝）需減緩毛髮生長，此處使用除臭劑及制汗 ^以控制腋毛内所產生之氣味。期望有可供腋下使用之產 品，在除臭或制汗活性之同時具有減緩毛髮生長活性。 非裔型毛髮形態獨特—具有不同直徑之捲曲毛幹。此 種複雜之毛幹結構需要特殊之修饰產品及方法，以確定非 -4- 1紙張尺度適用中國國家標準（CNS)A4規格（21Q X挪公爱）—-------— 592718 經濟部智慧財產局員工消費合作社印製 A7 _B7_五、發明說明（3 ) 裔型毛髮保持美觀上所需之性質。期望有一種產品，可降 低此種複雜性，使該非裔型毛髮更易處理，並改善其外 觀。 期望提供一種方法，以化學或自然地影響毛髮生長、 毛囊及毛幹尺寸及毛幹色素沉著，而不對使用者產生不需 要之副作用。 發明概述 根據本發明，吾人已發現一種組合物及方法，以改變 哺乳類毛髮生長、毛囊及毛幹尺寸及毛髮色素沉著，其係 於哺乳類皮膚上局部施用有效量之局部活性組合物，包含 蛋白酶抑制劑、植物萃取物，尤其是豆科植物萃取物包括 但不限於豆漿，歷經足以影響毛髮生長、毛囊及毛幹尺寸 及毛幹色素沉著之時間。該種局部活性組合物可另外組合 以其他活性成分，包括但不限於合成或天然異黃酮，以促 進針對毛髮生長及色素沉著所期望之效果。 本發明組合物及方法提供一種獨特而簡便之方式，以 減緩毛髮生長、縮小毛囊及毛幹尺寸並減少毛幹色素沉 著，其係使用含有絲胺酸蛋白酶抑制活性之絲胺酸蛋白 抑制劑、豆科植物之植物萃取物，尤其但不限於豆聚，及 其與異黃酮之組合物。 圖式簡單說明 本發明含有至少一種彩色圖型。此種具有彩色圖型之 (請先閱讀背面之注意事項再填寫本頁) 裝Hair = Combination, this method can promote and prolong the effect of hair removal, and reduce and reduce the demand and frequency of hair removal. Long-term use of the results of this study can make hair grow thinner, softer and reduce pigmentation printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs, making it less noticeable without the need to use other hair removal methods. The armpit (armpit) needs to slow down hair growth. Here, deodorants and sweat ^ are used to control the odor generated in the armpit hair. Products for underarm use are expected to have a hair-reducing activity while deodorizing or sweating activity. Afro-type hair has a unique shape—curly hair shafts with different diameters. This complex hair shaft structure requires special modified products and methods to determine that the non--4- 1 paper size is applicable to the Chinese National Standard (CNS) A4 specification (21Q X Norwegian public love) ----------- 592718 Printed by A7 _B7_ of the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs. 5. Description of the Invention (3) The properties of the hair of the descent type to maintain the aesthetic appearance. It would be desirable to have a product that would reduce this complexity, make the afro hair easier to handle, and improve its appearance. It is desirable to provide a method to chemically or naturally affect hair growth, hair follicle and hair shaft size, and hair shaft pigmentation without causing unwanted side effects to the user. SUMMARY OF THE INVENTION In accordance with the present invention, we have discovered a composition and method for altering mammalian hair growth, hair follicle and hair shaft size, and hair pigmentation, which is a topically applied effective amount of a topically active composition on mammalian skin, including protease inhibition Agents, plant extracts, especially leguminous plant extracts include, but are not limited to, soy milk, and have experienced a time sufficient to affect hair growth, hair follicle and hair shaft size, and hair shaft pigmentation. This topically active composition may be combined with other active ingredients, including but not limited to synthetic or natural isoflavones, to promote the desired effects on hair growth and pigmentation. The composition and method of the present invention provide a unique and simple way to slow down hair growth, reduce the size of hair follicles and hair shafts, and reduce pigmentation of hair shafts. It uses a serine protein inhibitor containing serine protease inhibitory activity, Plant extracts of leguminous plants, in particular but not limited to soya beans, and combinations thereof with isoflavones. Brief Description of the Drawings The present invention contains at least one color drawing. This kind of color pattern (please read the precautions on the back before filling this page)

- > 1 n _1 ^ ^ · ·1_— n I n in 11 I % 本紙張尺度適用中國國家標準（CNS)A4規格（210 X 297公釐） 592718-> 1 n _1 ^ ^ · · 1_— n I n in 11 I% This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) 592718

經濟部智慧財產局員工消費合作社印製 五、發明說明（4) 專利的副本係於請求及付出必要費用下由專利商標局提 供。 參照以下本發明詳述及附圖可進一步明瞭本發明及其 他優點，其中： 圖1 :對照組及經豆漿處理之C57B1/6老鼠毛髮的相 片（1¾倍率）。 圖2 :對照組及經豆漿處理之C57B1/6老氟毛幹於毛 髮周期第四曰之組織剖面圖。 圖3 :對照組及經豆漿處理之C57B1/6老鼠毛幹於毛 髮周期第七日之組織剖面圖，高及低倍率。 圖4 ··對照組及經豆漿處理之C57B1/6老鼠毛幹於毛 髮周期第18日之組織剖面圖。 圖5 :對照組及經豆漿處理之C57B1/6老鼠毛幹於毛 髮周期第21日之組織剖面圖。 圖6:對照組、經豆漿處理及經豆漿衍化蛋白質處理 之C3H老鼠毛幹（高倍率）於毛髮周期第七日之組織剖面 圖。 圖7:對照組、經豆漿處理及經豆漿衍化蛋白質處理 之C3H老鼠毛幹(低倍率）於毛髮周期第七曰之組織剖面 圖。 圖8:對照組、經豆漿處理及經豆漿衍化蛋白質處理 之C3H老鼠毛幹於毛髮周期第21曰之組織剖面圖。 圖9 :對照組及經豆漿衍化蛋白質處理之C3H老鼠毛 髮之相片（高倍率）。 -6- 本紙張尺度適用中國國豕標準（CNS)A4規格（210 X 297公爱） f—— (請先閲讀背面之注意事項再填寫本頁) . 592718 A7 B7 五、發明說明（5) 圖1〇:對照組及經豆漿衍化蛋白質處理之C57B1/6 老鼠毛幹（高倍率)於毛髮周期第八日之組織剖面圖。 (請先閱讀背面之注意事項再填寫本頁) 圖11 :說明豆漿之色胺酸抑制活性的圖。 圖12:C57Bl/6老鼠皮膚於整個毛髮周期之西方印潰 法’說明於豆漿處理後降低之酪胺酸酶及TRP-1蛋白質濃 度。 圖13 :人類顏面未經處理及經豆漿處理側面的相 片，每日使用豆漿處理歷經四週。 圖14:使用及不使用豆漿處理時毛幹尺寸定量分 析。 圖15 :於一腿部使用豆漿處理五週之後的人類腿部 毛髮相片。 圖16 ··對照組、經豆漿處理、及經富含異黃酮之豆 漿處理之C57B1/6老鼠毛髮（高倍率）的相片。 ·% 圖Π ··對照組、經豆漿處理、及經富含異黃酮之豆 漿處理之C57B1/6老鼠皮膚於毛髮周期第15曰之組織剖 面圖，說明毛幹之厚度及顏色。 經濟部智慧財產局員工消費合作社印製 圖18:使用各種豆漿及異黃酮調配物處理三週後， C57B1/6老鼠毛髮之相片。 體實例詳述 本發明中，“哺乳類，，應包括任何“高級脊椎動物包括 哺乳動物類”，如Webster氏醫學字典407(1986)所定義， 包括但不限於人類。本發明所使用之“(百分比，重量/體 -7- 本紙張尺度適用中國國家標準（CNS)A4規袼咖x 297公髮） 592718 A7 經濟部智慧財產局員工消費合作社印製Printed by the Consumer Cooperatives of the Intellectual Property Office of the Ministry of Economic Affairs 5. Description of the invention (4) A copy of the patent is provided by the Patent and Trademark Office upon request and payment of necessary fees. The present invention and other advantages can be further understood with reference to the following detailed description of the present invention and the accompanying drawings, wherein: Fig. 1: Photographs of the control group and soybean milk-treated C57B1 / 6 mouse hair (1¾ magnification). Figure 2: Cross-section of the control group and the soybean milk-treated C57B1 / 6 old fluorine hair shaft during the fourth cycle of the hair cycle. Figure 3: Tissue cross-sections of the control group and soybean milk-treated C57B1 / 6 mice on the seventh day of the hair cycle, high and low magnification. Figure 4 · Cross-sections of the control group and soybean milk-treated C57B1 / 6 mice's hair shaft on the 18th day of the hair cycle. Figure 5: Cross-section of the control group and soybean milk-treated C57B1 / 6 mice's hair shaft on the 21st day of the hair cycle. Figure 6: Cross section of the hair shaft (high magnification) of the control, C3H mice treated with soybean milk and protein derived from soybean milk on the seventh day of the hair cycle. Figure 7: Cross section of the hair shaft (low magnification) of the control group, soymilk-treated and soymilk-derived protein-treated C3H mice during the seventh day of the hair cycle. Figure 8: Cross-sectional view of the tissues of the control group, soymilk-treated and soymilk-derived protein-treated C3H mice during the 21st day of the hair cycle. Figure 9: Photographs (high magnification) of the hair of the control group and C3H mice treated with soy milk-derived protein. -6- This paper size applies to China National Standard (CNS) A4 (210 X 297 public love) f—— (Please read the precautions on the back before filling this page). 592718 A7 B7 V. Description of the invention (5) Figure 10: Cross-sections of the control group and soybean milk derived protein-treated C57B1 / 6 mice hair shaft (high magnification) on the eighth day of the hair cycle. (Please read the precautions on the back before filling out this page) Figure 11: A graph illustrating tryptophan inhibitory activity of soy milk. Figure 12: Western blot method of C57Bl / 6 mouse skin throughout the hair cycle 'illustrates the reduced tyrosinase and TRP-1 protein concentrations after soymilk treatment. Figure 13: Untreated and soymilk-treated side faces of the human face. Soymilk was used daily for four weeks. Figure 14: Quantitative analysis of hair shaft size with and without soybean milk treatment. Figure 15: Photo of human leg hair after five weeks of soy milk treatment on one leg. Figure 16 · Photographs of hair (high magnification) of the control group, C57B1 / 6 mice treated with soy milk, and isoflavone-rich soybean milk. ·% Figure Π · Sectional cross-section of the skin of the control group, soymilk-treated, and isoflavone-rich soybeanmilk-treated C57B1 / 6 mice during the 15th hair cycle, illustrating the thickness and color of the hair shaft. Printed by the Consumer Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs. Figure 18: Photo of C57B1 / 6 mouse hair after three weeks of treatment with various soymilk and isoflavone formulations. Detailed description of examples In the present invention, "mammals" shall include any "advanced vertebrates including mammals", as defined by Webster's Medical Dictionary 407 (1986), including but not limited to humans. "( Percentage, weight / body -7- This paper size applies to China National Standard (CNS) A4 Regulations x 297, issued 592718 A7 Printed by the Employees ’Cooperative of Intellectual Property Bureau, Ministry of Economic Affairs

B7 發明說明（6) 積）^曰母100毫升總组合物之特定成分克數。 劑及具===：：，_括蛋白醇抑制 =白酶抑制__===3。 色胺酸抑制劑(“STI，，) ^尤-'疋大豆 =::植物萃取物二 積計之較佳用::由Π白酶抑制_本發明組合物總體 ㈣/體積)至約“ 至約…體積）m糸由約0.001百分比(重量/體積) 將人！重量/體積)。較佳之植物含水萃取物諸如豆 水之3 1係10_99百分比(體積/體積），更佳係由5〇 9 分比(體積/體積）。 白 一吾人已意外地發現當局部活性劑諸如前述者—尤其是 豆襞或3有豆漿之調配物—富含異黃酮時，尤其是由大豆 衍化之異黃酮，可增進其對於毛髮生長、毛髮尺寸及毛^ 色素〉儿著之抑制效果。該植物含水萃取物諸如豆漿中之異 黃_含量較佳係0·000005-15百分比（體積/體積），更佳係 申0.00001_1〇百分比(體積/體積）。 若需要該局部活性醫藥或化粧劑之輸送參數，則本潑 明局部活性組合物可另外包含藥學上可接受或化粒學上3 接受之媒質，其可作為輸送系統，以使該局部活性劑渗/ 毛幹及皮膚内。 β 該醫藥或化粧組合物可視情況結合其他成分，諸如泪 溼劑、化粧佐劑、抗氧劑、脫色劑、抗老化劑、除毛劑、B7 Description of the Invention (6) Product ^ ^ 100 grams of the specific ingredients of the total composition of the mother 100 ml. Agents and tools ==== ::, _ inhibition of albuminol = inhibition of white enzyme __ === 3. Tryptophan Inhibitor ("STI ,," ^ You- '疋 Soybean = :: Preferred use of botanical extract diplomatism ::: Inhibition by white enzyme _ overall composition / volume of the present invention) to about " To about ... volume) m 糸 will be people from about 0.001% (weight / volume)! B). A preferred plant aqueous extract such as soybean water is a 31-series 10-99 percent (vol / vol), and a more preferred range is 509 (vol / vol). Bai Yiwu people have unexpectedly discovered that when topical agents such as the foregoing—especially tempeh or a soymilk formulation—are rich in isoflavones, especially isoflavones derived from soy, can increase their effect on hair growth and hair. Size and Hair ^ Pigment> Inhibition effect of children. The water-containing extract of the plant, such as the content of isoflavone in soybean milk, is preferably 0.0005-15% (v / v), and more preferably 0.00001-10% (v / v). If the delivery parameters of the topically active medicine or cosmetic are required, the present active topical composition may additionally include a pharmaceutically acceptable or granulated 3 acceptable medium, which can be used as a delivery system to enable the topical active agent Penetration / dry hair and skin. β The medicinal or cosmetic composition may be combined with other ingredients, such as a lacrimal agent, cosmetic adjuvant, antioxidant, decolorant, anti-aging agent, hair removal agent,

____|紅- \ k^) ' m ^ 11— L._. ir 1 i ‘，# ‘ "—%'th, ^L% Π tA 〆 !·裝____ | Red-\ k ^) 'm ^ 11— L._. Ir 1 i ‘, #‘ " —%' th, ^ L% Π tA 装! · 装

本紙國國^:標準（CNS)A4規格（210 X 297公釐 592718 A7 B7 五、發明說明（7 ) 經 濟 部 智 慧 財 產 局 消 費 合 作 社 印 製 頭髮造型#!、防晒劑、表面活_、發_、調理劑、保 渔劑、香料、著色劑、調化劑、緩衝劑、防腐劑等，及立 混合物。可於不影響該絲胺酸蛋自$性之用量下 等物質，以產生化粧或醫藥產品，諸如但不限於精油、乳 霜、洗劑、糊劑、凝膠、粉末、敷劑或注射物，以減缓毛 髮生長、毛髮尺寸及毛髮色素沉著。 本發明組合物可於其他活性成分或組合物之前、同時 或之後施用，以促進其效果。例如，本發明組合物可與一 或多種有利於除毛之產品結合使用，以實際移除毛髮，降 低毛髮可見度、改善毛髮風采或改善毛髮處理。本發明組 合物可於除毛之前、之期間或之後施用。其可與下列物質 中之-或多種同時局部施用：除毛劑、洗髮精、毛髮調理 7、造型凝膠、護髮產品、«產品、刺毛產品、除毛產 剃毛後產品、除臭劑、制汗劑、電針除毛、雷射除 毛、光導性除毛、敷劑或沐浴添加劑。 本發明組合物可每日施用至少歷經四至八週，如此應 可發現其對毛髮外觀之影響。可連續施用，先決條件為期 望保持該毛髮之狀態。每日施用於臉部可緩和假性鬚囊炎 及/或多毛症之狀況;施用於腋部可減缓腋下之毛髮生長， 施用於頭皮及頭髮上有助於非裔型毛髮之處理及造型。 ^該局部活性醫藥或化粧組合物之施加量應可有效地改 $哺礼類毛髮生長、毛囊及毛幹尺寸及毛幹色素沉著。本 毛明所使用之“有效量，，意指足以覆蓋皮膚表面期望延遲毛 <生長及毛髮色素沉著及縮小毛髮尺寸之區域之量。當期 訂 % -9- 本紙張尺度適用中@ g^5^NS)A4規格⑵〇 X 297公釐） 592718 A7 B7 五、發明說明（ 望延遲毛髮生長、毛髮尺寸及毛髮色素沉料，該組合物 較佳係施加於皮膚表面，使得基於每平方厘米皮膚表面存 有約2微升/厘米2至約500微升/厘米2之局部活性劑面子 吾人意外地發現當局部活性劑諸如豆漿或富含異黃明 之豆漿局部施加於動物皮膚時，明顯延遲毛髮生長、毛囊 及毛幹尺寸及毛幹色素沉著。吾人亦相信因為人類之毛髮 生長周期經常較老鼠緩慢，故於人類體内之毛髮生長延遲 遠長於老鼠。 本文所說明之發明可適當地於任何組份、成分、或未 詳細揭示於本發明之步驟不存在下進行。下文最示數個實 例以進一步說明本發明之性質及其進行方式。然而，本發 明應不限於其細節。 Λ 實例 宜試驗物質於老鼠系統中之除毛包 C57BI/6或C3H老鼠（雄性及雌性）係得自Charles River(Kingston，NY) ’ 8-10週大，處於其個別毛髮周期之 $止(靜止）期。毛髮生長係藉著個別動物背毛根據SteM 等人糖皮質激素對毛髮生長起始之影響:再審查”，6 Sk比 Phamiac〇L，l25-134(1993)所列示之方法的敷蠟除毛（拔除） 所誘t。8-10週大之C57B1/6及C3H老鼠於除毛時所有 毛囊皆同時開始生長期（毛髮生長期）。如表丨所示，於誘 發部位發現以下現象： --裝--- (請先閱讀背面之注意事項再填寫本頁) 訂！ 經濟部智慧財產局員工消費合作社印製 -10- 592718Country of this paper ^: Standard (CNS) A4 specification (210 X 297 mm 592718 A7 B7 V. Description of invention (7) Printed hair styling # !, sunscreen, surface activity_, hair_ , Conditioners, fisheries, flavoring agents, colorants, conditioners, buffers, preservatives, etc., and stand-alone mixtures. It can be used to produce makeup or other substances in an amount that does not affect the self-property of the serine egg. Pharmaceutical products such as, but not limited to, essential oils, creams, lotions, pastes, gels, powders, dressings, or injectables to slow down hair growth, hair size, and hair pigmentation. Compositions of the invention can be used in other activities Ingredients or compositions are applied before, at the same time or after, to promote their effect. For example, the composition of the present invention can be used in combination with one or more products that facilitate hair removal to actually remove hair, reduce hair visibility, improve hair style or Improves hair treatment. The composition of the present invention can be applied before, during or after hair removal. It can be applied topically with one or more of the following: hair removal agents, shampoos, hair tones Management 7, styling gel, hair care products, «products, bristles products, hair removal products after shaving, deodorants, sweat inhibitors, electroacupuncture hair removal, laser hair removal, photoconductive hair removal, application Agent or bath additive. The composition of the present invention can be applied daily for at least four to eight weeks, so that its effect on the appearance of the hair should be found. It can be applied continuously, provided that it is desired to maintain the condition of the hair. Daily application to the face Can alleviate the condition of pseudostigmatitis and / or hirsutism; applied to the axillary can slow the growth of underarm hair, and can be applied to the scalp and hair to help the treatment and styling of African-style hair The application amount of the medicinal or cosmetic composition should be able to effectively modify the hair growth, hair follicle and hair shaft size and hair shaft pigmentation of "beneficial hairs." The "effective amount" used in this hair Ming means a sufficient delay to cover the skin surface. Hair & Amount of hair growth and hair pigmentation and reduction of hair size. Current order% -9- This paper size applies @ g ^ 5 ^ NS) A4 size ⑵〇X 297 mm) 592718 A7 B7 V. DESCRIPTION OF THE INVENTION For hair size and hair pigmentation, the composition is preferably applied to the skin surface such that a topical active agent of about 2 μl / cm 2 to about 500 μl / cm 2 is stored on a per cm2 skin surface. It was found that when topically active agents such as soy milk or soy milk rich in isoxamine are topically applied to animal skin, it significantly delays hair growth, hair follicle and hair shaft size, and hair shaft pigmentation. I also believe that human hair growth is often more frequent than mice. Slow, so the hair growth delay in humans is much longer than in mice. The invention described herein can be suitably performed in the absence of any component, ingredient, or step that is not disclosed in detail in the present invention. Several examples are shown below. In order to further explain the nature of the present invention and how it is performed. However, the invention should not be limited to its details. Λ Examples should be tested in a mouse system for hair removal packets C57BI / 6 or C3H mice (male and female) obtained from Charles River (Kingston, NY) '8-10 weeks old, at the end of their individual hair cycle ( Inactivity) period. Hair growth is the effect of wax removal by the method shown in SteM et al. On the initiation of hair growth according to SteM et al. Glucocorticoids: 6 Sk than Phiacocol, 12-25-134 (1993) All hair follicles attracted by hair (pulling) t. 8-10 weeks old C57B1 / 6 and C3H mice at the same time all hair follicles began to grow at the same time (prolonged hair growth). As shown in Table 丨, the following phenomenon was found at the induced site: --Install --- (Please read the precautions on the back before filling out this page) Order! Printed by the Consumer Cooperative of the Intellectual Property Bureau of the Ministry of Economy-10-592718

經濟部智慧財產局員工消費合作社印製 誘導後之曰數 態及組織發現 1-2(前生長期） 開始生長新毛囊 3至4 H專主曼展，但其毛幹尚未可見 7至8(後生長期） 查复兔色^有極黑之皮膚;組織上可見其毛幹 11-12 毛幹開始穿透表皮 14 每隻老鼠皆覆有短毛 19 組織上發現毛囊退化（生長中期） 21-25 毛囊退化至休止期 (請先閱讀背面之注意事項再填寫本頁) 如表1所示，當除毛後數曰動物粉紅色皮膚開始變黑 時，可見到毛髮生長。此因該C57BI/6及C3H老鼠僅於 毛囊中含有黑素細胞，而背部表皮中則否，故毛幹中有毛 髮色素沉著。使用市售產品進行化學除毛時，吾人國際申 清案編號PCT/US 97/11033中列示相同之毛髮生長特性。 因為鼠類毛髮周期不僅因種類不同而異，個別動物之 間亦有差異，研究時於每隻動物體内分析毛髮周期之狀 態。使用剪刀自每隻老鼠身上取下2厘米乘〗厘米皮膚試 樣，於25 C下使用pH約6.9-7.1之10百分比經緩衝甲駿 水溶液(Stephens Scientific)固定，根據已知方法成型於石 堪塊中。該蠟塊經切薄片，剖面使用H&E或Fontana-Mason染色法染色。組織性地檢測該剖面，使用技藝界眾 所周知之方法，以確定毛髮周期之期數、毛囊及毛幹尺寸 -11 - 本紙張尺度適財國國家標準（CNS)A4規格⑵0 x 297公釐)----------- f裝---------訂-------- 592718 A7 B7 經濟部智慧財產局員工消費合作社印製 五、發明說明（1G) 及毛髮色素沉著程度。目測及使用低倍率（X8)解剖顯微鏡 毛髮長度。 此實例及吾人國際申請案編號PCT/US 97/11033所述 者顯示平均約25曰之C57BI/6及C3H老鼠的毛髮生長周 期，並記錄毛囊及毛幹發展之相同時序，而與所使用之除 毛方法無關。 -實一例2:豆漿及豆漿調配物之製備 一種製造豆漿之方法係將大豆浸於去離子水或純水中 歷經數小時，於其完全水合之後進行研磨，添加少量水。 (研磨方法使得豆漿被萃取）。收集之後，可過濾該豆漿以 除去任何殘留之豆皮部分。使用於下述調配物之豆襞可為 前述新鮮豆漿，或可自大豆粉及水製得。該大豆粉係自大 豆研磨，亦可經冷凍乾燥、喷乾、或冷凍乾燥，彬成之豆 裝可經或不經過濾。所製備之豆漿可含有約1至約9〇重 i百分比之乾燥大豆粉末。另一實例係使用豆漿粉，由經 冷泉乾燥、噴乾或冷凍乾燥之豆漿製得，添加水或經過據 或均質化調理。亦可使用其他大豆萃取方法，以於下述調 配物中產生該活性成分。例如，該活性成分可使用乙醇/ 水混合物而自經研磨之大豆萃取，如此保留該大豆之絲胺 酸蛋白酶抑制活性，較佳係該蛋白質STI保持不變。 可使用於本發明之豆製品可製自所有大豆類，而與其 地理來源、曝晒、收割時間等因素無關。然而，以特定種 類、地理來源或生長條件較佳。例如但不限於其大豆胰^ ,, 本紙張f度適用國家標準（CNS：^4規辂⑽x 297公楚）— " ^^〜 广讀先聞讀背面之注意事項再填寫本頁} ^ ^--------^--------- % 592718 A7 ------— —_B7 五、發明說明（11 ) ' 〜^ 紅酶抑制劑(STI)含量或異黃_含量豐富之大豆種類 五類中之STI或異黃_含量豐富之生長條件較佳。應已知 可使用於本發明組合物中之大豆產品具有明 顯氣味，某此 _可讓人接受，某些則較不受歡迎。若需要，則本發= 組t物之氣味可藉著使用由已知可減少氣味之特定大^種 1衍生之大丑產品降低，包括但不限於脂肪氧合1缺乏 型大且及具有經修飾糖性質者等。於該調配物中降低氣濃 方法亦了減少氣味。亦可使用各種遮蔽劑或香料 蓋該氣味。 ^ ^本發明組合物可另外包含表面活性劑、潤溼劑、保溼 ^ i周理劑、香料、著色劑、防腐劑、抗氧劑、脫色劑、 除毛劑、抗老化劑、防晒劑、發泡劑、化粧佐劑、緩衡 或其混合物。 本發明組合物可停留於皮膚上歷經足以產生改變之時 間。例如，本發明組合物可應用於皮膚日常處理歷經至少 約四週’更佳係每日施加該組合物歷經至少八週。 本發明另一種方法係為減少或預防假性鬚囊炎之方 法。每日施加本發明組合物可減少或預防此種狀況。本發 明組合物亦可每日施加於該辅助區域以減緩毛髮生長。此 外，本發明組合物可每曰施加於頭皮，以造型或改善非裔 型毛髮之處理。 / 如吾人共待審美國專利申請案（代理文件編號JBP 430) 所示，可使用數種以大豆為主之調配物以減少色素沉著。 所有此等調配物亦可用於減緩毛髮生長。下表2中列示部 -13- 本紙張尺度適用中國國家標；^NS)A4規格⑽χ挪公餐) f請先閱讀背面之注意事項再填寫本頁}The number of states and organizations found after the induction of printing by the Consumer Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs and the organization found that 1-2 (prenatal period) began to grow new hair follicles. Post-growth period) Check rabbit color ^ has extremely dark skin; the hair shaft 11-12 can be seen on the tissue. The hair shaft begins to penetrate the epidermis. 14 Each mouse is covered with short hair. 25 Hair follicles degenerate to rest (please read the precautions on the back before filling this page) As shown in Table 1, when the animal's pink skin begins to turn black after hair removal, hair growth can be seen. This is because the C57BI / 6 and C3H mice only contain melanocytes in the hair follicles, but not in the epidermis on the back, so there is hair pigmentation in the hair shaft. When using commercially available products for chemical hair removal, the same hair growth characteristics are listed in our International Application No. PCT / US 97/11033. Because the rodent hair cycle differs not only from species to species, but also from animal to animal, the state of the hair cycle was analyzed in each animal during the study. Remove 2 cm by cm skin samples from each mouse using scissors, fix at 25 C using a buffered solution of Stephens Scientific (Stephens Scientific) at a pH of about 6.9-7.1 at 10%, and mold into Shikan according to known methods Block. The wax block was sliced, and the cross section was stained with H & E or Fontana-Mason staining. Organize the profile and use methods well known in the art world to determine the number of hair cycles, hair follicles, and hair shaft size-11-This paper is sized for the National Standard for Finance (CNS) A4 (0 x 297 mm)- ---------- f equipment --------- order -------- 592718 A7 B7 Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs V. Invention Description (1G) And hair pigmentation. Visually and using a low magnification (X8) dissecting microscope hair length. This example and my international application number PCT / US 97/11033 show an average hair growth cycle of C57BI / 6 and C3H mice of about 25 days, and record the same time series of hair follicle and hair shaft development, which are the same as those used Hair removal method is irrelevant. -Example 2: Preparation of Soymilk and Soymilk Preparations A method of making soybean milk is to immerse soybeans in deionized water or pure water for several hours, grind them after they are fully hydrated, and add a small amount of water. (Grinding method allows extraction of soy milk). After collection, the soy milk can be filtered to remove any remaining soy skin portions. The soy bean paste used in the following formulations may be the aforementioned fresh soybean milk, or may be made from soybean flour and water. The soybean powder is ground from soybeans, and can also be freeze-dried, spray-dried, or freeze-dried. Bincheng's beans can be used with or without filtering. The prepared soymilk may contain from about 1 to about 90% by weight dry soybean powder. Another example is the use of soybean milk powder, which is prepared from cold spring dried, spray-dried or freeze-dried soybean milk, added with water, or subjected to conditioning or homogenization. Other methods of soy extraction can also be used to produce the active ingredient in the formulations described below. For example, the active ingredient can be extracted from milled soybeans using an ethanol / water mixture, so that the soybean's serine protease inhibitory activity is retained, and preferably the protein STI remains unchanged. The soy products used in the present invention can be made from all soybeans, regardless of factors such as geographical origin, exposure, harvest time, and the like. However, it is better to be of specific species, geographical origin or growing conditions. For example, but not limited to its soybean pancreas ^, this paper f is applicable to national standards (CNS: ^ 4 Regulations x 297 Gongchu) — " ^^ ~ Read the notes on the back before reading this page and fill in this page} ^ ^ -------- ^ ---------% 592718 A7 -------- --_ B7 V. Description of the invention (11) '~ ^ Red enzyme inhibitor (STI) content or The growth conditions of STI or iso-yellow-rich soybeans in the five types of iso-yellow-rich soybeans are better. It should be known that the soybean products used in the compositions of the present invention have a distinct odor, some are acceptable, and some are less desirable. If required, the odor of this product can be reduced by using large ugly products derived from specific large species 1 known to reduce odor, including but not limited to fatty oxygenation 1 deficient and having Modified sugar properties and so on. The method of reducing gas concentration in the formulation also reduces odor. Various masking agents or fragrances can also be used to cover the scent. ^ ^ The composition of the present invention may further include a surfactant, a wetting agent, a moisturizing agent, a finishing agent, a fragrance, a coloring agent, a preservative, an antioxidant, a decolorizing agent, a hair removal agent, an anti-aging agent, and a sunscreen agent , Foaming agents, cosmetic adjuvants, slow balances or mixtures thereof. The composition of the present invention can remain on the skin for a time sufficient to effect a change. For example, the composition of the present invention can be applied to daily skin treatment for at least about four weeks', more preferably the composition is applied daily for at least eight weeks. Another method of the present invention is a method for reducing or preventing pseudo whiskers. Applying the composition of the present invention daily reduces or prevents such conditions. The composition of the invention can also be applied daily to this auxiliary area to slow down hair growth. In addition, the composition of the present invention can be applied to the scalp every day to shape or improve the treatment of African-style hair. / As shown in my co-pending US patent application (agent file number JBP 430), several soybean-based formulations can be used to reduce pigmentation. All of these formulations can also be used to slow hair growth. The parts listed in Table 2 below -13- This paper size applies to Chinese national standards; ^ NS) A4 size ⑽χ Norwegian public meal) f Please read the precautions on the back before filling in this page}

aMmm" mmm— · n 1« «1 ϋ at§ I 經濟部智慧財產局員工消費合作社印製 592718 A7 B7 五、發明說明（12) 分特佳之豆漿調配物及含有異黃酮之豆漿調配物的實例。 使用於本發明之異黃酮配製劑的實例係為來自 Ichimaru，Japan之黃固酮SE，含有約0.1百分比之純異黃 _。所有此等調配物中，豆聚皆可使用適量之豆粉或豆聚 粉及水置換。 表2:豆漿精油調配物： 經濟部智慧財產局員工消費合作社印製 大豆精油 1 6 8 21 22 23 24 25 26 豆漿 87.42% 89.04% 96.09% 96.05% 96.05% 95.70% 94.40% 94.40% 92.40% 苯氧基乙醇 0.73% 苯氧基乙醇及 帕若;^(paraben) 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 甘油 2.50% 2.50% 環*** (cyckxndhicaie) 2.00% 鋁澱粉琥珀酸 辛酯 0.75% 蔗糖椰酸酯 1.00% 1.00% PEG-6癸酸/ 辛酸三甘油酯 3.00% 3.00% 乙二胺四乙酸 二鈉 0.10% 0.10% 0.05% 0.05% 0.05% 0.05% 聚丙烯醯胺/月 桂醇-7/C13-14 異石蠟 2.50% 2.75% 2.90% 2.90% 2.90% 3.20% 3.50% 3.50% 3.50% 抗壞血酸 0.01% 1.00% 經丁基化之經 基甲苯 0.10% 0.01% 0.05% 0.05% 0.05% 0.05% 0.05% 0.05% 聚沿幽旨20 0.50% -14- (請先閱讀背面之注意事項再填寫本頁) .1裝 ϋ ϋ I 1 ϋ i I *1 ϋ I ϋ te· 本紙張尺度適用中國國家標準（CNS)A4規格（210 X 297公釐） 592718 A7 B7 13 μ月說明（ 乳鐵蛋白 1.00% 1.00% 1.00% 生育酚 1.00% 總量 100.00% 100.00% 100% 100% 100% 100% 100% 100% 100% 經濟部智慧財產局員工消費合作社印製 27 28 29 30 31 32 33 34 35 豆漿 90.70% 94.70% 85.7% 90.7% 93.70% 94.70% 苯氧基乙醇及 帕若本 (paraben) 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 甘油 5.00% 乙二胺四乙酸 二鈉 0.05% 0.05% 0.05% 0.05% 0.05% 0.05% 0.05% 0.05% 烤· 0.05% 聚丙婦醯胺/ 月桂醇-7/C13-14 異石 蠟 3.20% 3.20% 3.20% 3.20% 3.20% 3.20% 3.20% -1- 3.20% 3.20% 抗壞血酸 經丁基化之經 基曱笨 0.05% 0.05% 0.05% 0.05% 0.05% 0.05% 0.05% 0.05% 0.05% 去離子水 90.70% 90.70% 85.70% 紗寧200流體 1.00% 黃固酮SE 10.0% 5.00% 2.00% 1.00% 豆漿粉 5.00% 使用乙醇/水 混合物之大豆 萃取物 5% 10% 總量 100% 100% 100% 100% 100% 100% 100% 100% 100% -15- (請先閱讀背面之注音？事項再填寫本頁) 裝------ 0 本紙張尺度適用中國國家標準（CNS)A4規格（21〇 x 297公釐） 592718 經濟部智慧財產局員工消費合作社印製 A7 實例3 :含有由大之蛋仓酶抑制劑之局部 物的Μ借 口 購自Sigma-Aldrich Corporation之大豆胰蛋白酶抑制 劑（STI)及Bowman-Birk抑制劑(腦)混合於pH7 4之〇 Γ Μ經填酸鹽緩衝之鹽水<pBS，Gibc〇_BRL， GaitherSburg，MA)中，濃度為丨百分比至〇 〇〇1百分比。四 份體積之形成之溶液與1份體積之(100毫克/毫升)脂質體 媒貝此合’ ό亥媒質係精Niemiec等人所述之方法製備，以 產生該局部活性組合物。非離子性脂質體配製劑諸如 Niemiec等人“非離子性脂質體組合物對於胜肽藥物進入毛 囊-皮J3曰腺早位之局部輸送的影響:使用Hamster Ear模型進 行體内研究”，12Pharai.Res.ll84-88(1995)(“Niemiec)提及 之方式完全併入本發明一所揭示係技藝界所熟知，描述吾 人專利申請案（代理文件編號JBP 430)。GDL脂質體係如 前述Niemiec等人所述般地製備，不同處係以下變化:該非 離子性脂質體調配物含有甘油二月桂酸酯（£111111^故 GDL，ISP Van Dyk)/脂固醇(Cr〇da)/聚氧化乙烯_1〇•硬脂醚 (Bdj76，ICI)/聚氧化乙烯-9-月桂醚，比例為 37·5:12·5:33·3:16·7。使用 PBS 或 Hepes 緩衝 ^ 0.〇5M，pH7.4(Gibco-BRL of Gaithersburg，MD)作為製備該月匕 質體所使用之水相。 實例4 :豆舉里—壤毛髮生長类縮小^毛幹尺寸美塵小 毛髮色素沉著 -裳--- (諝先閱讀背面之注咅^事項再填寫本頁) 訂： 16- 592718aMmm " mmm— · n 1 «« 1 ϋ at§ I Printed by the Employees ’Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs 592718 A7 B7 V. Description of the invention (12) Examples of the special soymilk preparations and isoflavone-containing soymilk preparations . An example of an isoflavone formulation for use in the present invention is a flavonoid SE from Ichimaru, Japan, containing about 0.1% pure isoflavone. In all of these formulations, Doudou may be replaced with an appropriate amount of Soya flour or Soya flour and water. Table 2: Soymilk essential oil preparations: Soybean oil printed by the Consumer Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs 1 6 8 21 22 23 24 25 26 Soymilk 87.42% 89.04% 96.09% 96.05% 96.05% 95.70% 94.40% 94.40% 92.40% phenoxy Ethyl alcohol 0.73% phenoxyethanol and paraffin; ^ (paraben) 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% glycerol 2.50% 2.50% cyckxndhicaie 2.00% aluminum starch octyl succinate 0.75 % Sucrose cocoate 1.00% 1.00% PEG-6 capric acid / caprylic triglyceride 3.00% 3.00% disodium ethylenediamine tetraacetate 0.10% 0.10% 0.05% 0.05% 0.05% 0.05% polyacrylamide / lauryl alcohol- 7 / C13-14 isoparaffin 2.50% 2.75% 2.90% 2.90% 2.90% 3.20% 3.50% 3.50% 3.50% ascorbic acid 0.01% 1.00% butylated methyl toluene 0.10% 0.01% 0.05% 0.05% 0.05% 0.05% 0.05% 0.05% Juyanyouzhi 20 0.50% -14- (Please read the precautions on the back before filling out this page) .1 Installation ϋ I 1 ϋ i I * 1 ϋ I ϋ te · This paper size applies to China Standard (CNS) A4 size (210 X 297 male 592718 A7 B7 13 μ month description (lactoferrin 1.00% 1.00% 1.00% tocopherol 1.00% total 100.00% 100.00% 100% 100% 100% 100% 100% 100% 100% Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs Printed 27 28 29 30 31 32 33 34 35 Soymilk 90.70% 94.70% 85.7% 90.7% 93.70% 94.70% Phenoxyethanol and paraben 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00 % 1.00% Glycerin 5.00% Disodium ethylenediamine tetraacetate 0.05% 0.05% 0.05% 0.05% 0.05% 0.05% 0.05% 0.05% Baked 0.05% Polyprostol / lauryl-7 / C13-14 isoparaffin 3.20% 3.20% 3.20% 3.20% 3.20% 3.20% 3.20% -1- 3.20% 3.20% butylated ascorbic acid based 0.05% 0.05% 0.05% 0.05% 0.05% 0.05% 0.05% 0.05% 0.05% 0.05% deionized water 90.70% 90.70% 85.70% Sanon 200 fluid 1.00% Flavone SE 10.0% 5.00% 2.00% 1.00% Soymilk flour 5.00% Soy extract using ethanol / water mixture 5% 10% Total 100% 100% 100% 100 % 100% 100% 100% 100% 100% -15- (Please read the note on the back first ? Please fill in this page for more details) Packing ------ 0 This paper size is applicable to China National Standard (CNS) A4 (21 × 297 mm) 592718 Printed by A7, Employees' Cooperative of Intellectual Property Bureau, Ministry of Economic Affairs Example 3: Contains Soybean Trypsin Inhibitor (STI) and Bowman-Birk Inhibitor (Brain) purchased from Sigma-Aldrich Corporation under the pretext of MG, mixed with pH 7.4. In buffered saline (pBS, Gibac_BRL, GaitherSburg, MA), the concentration ranges from% to 0.001%. Four parts by volume of the resulting solution and 1 part by volume (100 mg / ml) of liposomes were prepared by the method described in Niemiec et al. To produce the topically active composition. Nonionic liposome formulations such as Niemiec et al. "Effects of Nonionic Liposomal Compositions on Local Delivery of Peptide Drugs into Hair Follicle-Skin J3: Early Glandular Disorders: In vivo Study Using the Hamster Ear Model", 12 Pharai. Res. Ll84-88 (1995) ("Niemiec") is fully incorporated into the method disclosed in the present invention, which is well known in the art world and describes our patent application (agent file number JBP 430). The GDL lipid system is as described in Niemiec et al. Prepared as described, the following changes in different places: the non-ionic liposome formulation contains glyceryl dilaurate (£ 111111 ^ GDL, ISP Van Dyk) / sterol (CrOda) / polyethylene oxide _1〇 • Stearyl ether (Bdj76, ICI) / polyoxyethylene-9-lauryl ether, the ratio is 37 · 5: 12 · 5: 33 · 3: 16 · 7. Use PBS or Hepes to buffer ^ 0.〇5M , PH7.4 (Gibco-BRL of Gaithersburg, MD) as the aqueous phase used to prepare the moon plastids. Example 4: Douguri-soil hair growth category shrinks ^ hair shaft size beautiful dust small hair pigmentation-clothes --- (谞 Please read the notes on the back 咅 ^ Matters before filling out this page) Order: 16- 592718

經濟部智慧財產局員工消費合作社印製Printed by the Consumer Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs

卜57B1/6老鼠係如實例1所述般地誘導新毛髮周期， 每日使用豆漿處理。每日觀察動物之毛髮生長情況，於該 毛髮周期之重要時間點取下皮膚組織切片。錢豆激處= 之結果’延遲毛髮生長，可見到其毛髮變得稀薄，觸感較 為光滑。如同預期地，經處理之老鼠於該毛髮周期之第孓 8日未顯不皮膚變黑，且與對照組動物不同地，於第 12日未見到其毛幹。經豆漿處理之老鼠的毛髮周期平均 延遲3-6日。圖丨係為老鼠毛皮之圖型，顯示於豆漿處理 後之毛髮外觀、顏色、尺寸及厚度差異。 組織切片皮膚試樣之組織檢驗確定此等觀察結果。如 F〇ntana-Mas〇n(F&M)染色之圖2所示，於毛髮周期之第四 日，未經處理之毛囊如同預期地完全發展，含有所有之細 胞層及色素澱積。相反地，經豆漿處理之試樣（於相同倍 率下）顯示較小而未完全發展之毛囊，而無色素澱積。 圖3係顯示兩組使用f&m染色之組織剖面，其為低 及高倍率。此等剖面係得自毛髮周期之第七日。上層顯示 在與未經處理之對照組比較之下，經豆漿處理之皮膚具有 較小、較短且較少色素沉著之毛囊。下層顯示較高倍率之 毛囊，進一步說明經豆漿處理之後的毛囊及毛幹尺寸及色 素沉著差異。 圖4顯示於毛髮周期第18曰時之經F&M染色皮膚的 低倍率剖面圖。於此倍率下，顯然該立漿處理使得毛囊尺 寸縮小’而導致毛幹長度及厚度縮小，並使得經處理毛囊 之整體色素沉著減少。 -17- (請先閱讀背面之注意事項再填寫本頁) 1裝·！丨！ί I訂·！ · 0, 本紙張尺度適用中國國家標準（CNS)A4規格（210 X 297公釐） 592718Bu 57B1 / 6 mice induced a new hair cycle as described in Example 1, and were treated daily with soy milk. The hair growth of the animals was observed daily, and skin tissue sections were removed at important points in the hair cycle. Money bean excitement = Result 'Delayed hair growth, it can be seen that the hair becomes thinner and feels smoother. As expected, the treated mice showed no darkening of the skin on the 28th day of the hair cycle, and unlike the control animals, their hair shafts were not seen on the 12th day. The hair cycle of mice treated with soybean milk was delayed by an average of 3-6 days. Figure 丨 is a picture of mouse fur, showing the difference in appearance, color, size and thickness of hair after soy milk treatment. Tissue examination of tissue samples of skin samples confirms these observations. As shown in Figure 2 of Fontana-Masson (F & M) staining, on the fourth day of the hair cycle, the untreated hair follicles developed completely as expected, containing all the cell layers and pigmentation. In contrast, the samples treated with soy milk (at the same magnification) showed smaller, incompletely developed hair follicles without pigmentation. Figure 3 shows the cross-sections of tissues stained with f & m, which are low and high magnifications. These profiles are derived from the seventh day of the hair cycle. The upper layer shows that compared with the untreated control group, the skin treated with soy milk has smaller, shorter and less pigmented hair follicles. The lower layer shows higher magnification of hair follicles, further illustrating the differences in hair follicle and hair shaft size and pigmentation after soy milk treatment. Figure 4 shows a low magnification cross-section of F & M-stained skin at the 18th day of the hair cycle. At this magnification, it is clear that the standing pulp treatment reduces the size of the hair follicles, resulting in a reduction in the length and thickness of the hair shaft, and reduces the overall pigmentation of the treated hair follicles. -17- (Please read the precautions on the back before filling this page) 1 pack丨! ί I order! · 0, This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) 592718

圖5係顯示兩倍率下之毛髮周期第21曰的皮膚剖 面二上層說明對照動物係處於生長中期，此時毛囊退化° 經豆漿處理之毛囊已完成生長中期，因其已顯 、 ，休止期。此情況顯示經豆漿處理之後，不僅延 味髮周期，亦使其提早終止。下層者於高倍率下說明該 長中期對照毛囊及較短、靜止期(休止期)經减處理： 囊。 尤毛髮生長、厶寸及色素沉荖之聚響^^ f 3H老鼠體内再現 為了證實丑漿對於毛髮生長之影響並非C57B1/6老鼠 所特有，吾人使用棕毛(Ag〇uti)C3H老鼠重複實例4所述 之實驗。此等實驗之結果於目測及組織上皆相同。豆漿於 C3H老鼠體内延遲毛髮生長並縮小毛囊及毛幹尺寸及色 沉著。 ” 、、且織为析確疋.此荨目測結果。如圖6之上層所示，使 用F&M染色，於該毛髮周期之第七日，經豆漿處理之毛 囊在與未經處理之對照組比較之下，較小而累積較少色 素圖7之上層（F&M染色）顯示較低倍率之相同皮膚剖 面说明經豆漿處理之後，較稀薄而較少色素沉著之毛 囊。 圖8係顯示於毛髮周期第21曰時經f&M染色之皮膚 剖面。如C57B1/6老鼠所示，經豆漿處理之後，該毛髮周 期提早終止。經豆漿處理之毛囊係處於休止期 ，而未經處 -18- 本紙張尺度剌+¾家標準（CNS)A4規格（210 X 297公 裝— (請先閱讀背面之注意事項再填寫本頁) 訂· 經濟部智慧財產局員工消費合作社印製 592718 經濟部智慧財產局員工消費合作社印製 五、發明說明（ 17 理之對照毛囊仍處於生長中期 實16二^衆·互鱼孚衍生之絲胺酸蛋白 复生·^尺寸及色素沉荖之^^^ 研究豆漿對於毛髮生長之影響的機制之中，吾人試驗 由豆漿衍生之絲胺酸蛋白酶抑制劑STI及BBI的效果。五 人早期已證實此等蛋白質藉著影響PAR-2路徑而於皮& 中誘發脫色（美國專利申請案，代理文件編號jBp 43〇)。 實例4所述之實驗使用STI、BBI及豆漿重複。阳 及BBI如實例3所述般地使用於PBS-脂質體媒質中。與 豆漿相同地，該毛髮周期中之視覺觀察確定STI及 可延遲毛髮生長，並縮小毛囊及毛幹尺寸（參照圖9之毛 髮圖片）。使用高濃度之STI及BBI，對於毛髮生長及色 素沉著之影響極為實質。 組織分析確定此等發現。如圖6所示，於毛髮周期之 第七日，1百分比之ST1及1百分比之BBI於C3H老鼠 體内縮小毛囊及毛幹尺寸並減少毛幹色素沉著。圖7顯示 該毛髮周期之同一日的低倍率剖面，說明相對於未經處理 之對照組，使用豆漿、STI或BBI處理之毛囊及毛幹較小 並色素沉著較少。圖10顯示STI及BBI亦於C57B1/6老 鼠體内具有相同效果，證明有較小且較少色素沉著之毛 囊。綜合起來，此實例顯示豆漿中所含之STI及BBI係為 由大豆衍生之絲胺酸蛋白酶抑制劑，可延遲毛髮生長，縮 小毛囊及毛幹尺寸，並減少毛髮色素沉著。STI及BBI代 -19- 本紙張尺度適用中國國家標準（CNS)A4規格（210 X 297公釐） (請先閱讀背面之注意事項再填寫本頁)Figure 5 shows the skin section of the 21st hair cycle at twice the rate. The upper two layers show that the control animal is in the middle stage of growth, at which time the hair follicles have been degraded. This situation shows that after the soymilk treatment, not only the taste period is delayed, but it is also terminated early. The lower stratum explained the long-to-mid-term control hair follicles and the shorter, quiescent (resting) periods with reduced treatment at high magnification: bursa. The reverberations of hair growth, size, and pigmentation ^^ f 3H mice reproduction In order to confirm that the effect of ugly pulp on hair growth is not unique to C57B1 / 6 mice, we repeated the examples using brown hair (Ag〇uti) C3H mice The experiment described in 4. The results of these experiments were visually and organizationally the same. Soymilk delayed hair growth and reduced hair follicle and hair shaft size and pigmentation in C3H mice. The results of visual inspection of this net. As shown in the upper layer of Figure 6, using F & M dyeing, on the seventh day of the hair cycle, the soy milk-treated hair follicles were compared with the untreated ones. In the group comparison, the smaller and less pigment accumulation. Figure 7 The upper layer (F & M staining) shows the same skin profile at a lower magnification, indicating that after soy milk treatment, the hair follicles are thinner and less pigmented. Figure 8 shows The section of the skin stained with f & M at the 21st of the hair cycle. As shown in C57B1 / 6 mice, the hair cycle was terminated prematurely after soy milk treatment. The soy milk-treated hair follicles were in a resting phase without treatment- 18- Size of this paper 剌 + ¾Home Standard (CNS) A4 specification (210 X 297 public packing — (Please read the notes on the back before filling out this page) Order · Printed by the Intellectual Property Bureau of the Ministry of Economic Affairs and Consumer Cooperatives 592718 Ministry of Economic Affairs Printed by the Intellectual Property Bureau's Consumer Cooperatives. V. Invention Description (17) The control hair follicles are still in the middle stage of growth. 162. Regeneration of serine protein derived from Huyufu. ^ Size and pigmentation. ^^^ Research Soy milk for hair Among the mechanisms of the effects of growth, we have tested the effects of soymilk-derived serine protease inhibitors STI and BBI. Five people have previously confirmed that these proteins induce discoloration in the skin & by affecting the PAR-2 pathway. (U.S. patent application, proxy file number jBp 43〇). The experiment described in Example 4 was repeated using STI, BBI, and soybean milk. The male and BBI were used in the PBS-liposome medium as described in Example 3. Same as soybean milk. The visual observation during this hair cycle determined that STI and delayed hair growth, and reduced the size of hair follicles and hair shafts (refer to the hair picture in Figure 9). The use of high concentrations of STI and BBI has a great impact on hair growth and pigmentation. In fact, tissue analysis confirmed these findings. As shown in Figure 6, on the seventh day of the hair cycle, 1% of ST1 and 1% of BBI reduced the size of hair follicles and hair shafts and reduced hair shaft pigmentation in C3H mice. Figure 7 shows a low magnification profile on the same day of the hair cycle, illustrating that compared to the untreated control group, hair follicles and hair shafts treated with soy milk, STI or BBI are smaller and less pigmented. Figure 10 shows that STI and BBI also have the same effect in C57B1 / 6 mice, demonstrating that there are smaller and less pigmented hair follicles. Taken together, this example shows that the STI and BBI contained in soybean milk are derived from soybeans Serine protease inhibitor, can delay hair growth, reduce the size of hair follicles and hair shafts, and reduce hair pigmentation. STI and BBI generation-19- This paper size applies to Chinese National Standard (CNS) A4 (210 X 297) Li) (Please read the notes on the back before filling this page)

mmmmB n I · ϋ n Βϋ ϋ ϋ «ϋ I 鲁· 592718mmmmB n I · ϋ n Βϋ ϋ ϋ «ϋ I Lu · 592718

經濟部中央標準局員工消費合作社印製 表旦漿成分中影響毛髮生長之一部分。 為了支持豆漿中之STI及bbi涉及前述毛髮生長效應 之叙說’口人喊驗豆漿之絲胺酸蛋白酶抑制活性。使用 “EnZChek”進行酶檢定，其係一種由M〇lecular Probe of Eugene’OR所製造之蛋白酶消化螢光試驗系統。使用1⑻ 單位之膜蛋白酶（購自Sigma chemicals，St丄ouis M0)，該 試驗系統產生約11〇〇單位之螢光讀數。此種反應係使用 漸增濃度之STI抑制，如已知之胰蛋白酶抑制劑所預測。 丑衆之連績稀釋物於此檢定中測試，發現其抑制胰蛋白酶 活性。如圖11所示，豆漿所具之胰蛋白酶抑制活性如同 約〇·2百分比純度之STI。顯示豆漿自身之毛髮生長效應 至少有一部分係來自STI及BBI。 免柄7 :签星^酪胺酸酶及TRP-1蛋白質表現之汝纒 上述貫例所述經豆漿處理之皮膚樣本的組織學分析顯 現毛囊内色素沉積減少。為進一步暸解豆漿誘發之去色素 作用的機制’吾等研究黑色素生成中之關鍵酵素酪胺酸酶 及安定絡胺酸酶之酵素酪胺酸酶相關之蛋白質（TRp_ 1)。如上述處理C57B1/6及C3H鼠，於研究中全程收集 樣本以供蛋白質分析用。使用標準程序，如Juan s. Bonifadno等人編輯之細胞生物學第六章：電泳及免疫墨 潰中目前之程序（1999年著作權：John Wiley & Sons，Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs. In order to support the narration of STI and bbi in soy milk related to the aforementioned hair growth effect, the mouth is called to test the serine protease inhibitory activity of soy milk. "EnZChek" was used for the enzyme assay, which is a protease digestion fluorescence test system manufactured by the Molecular Probe of Eugene'OR. Using 1⑻ units of membrane protease (purchased from Sigma chemicals, St 丄 ouis M0), the test system produced a fluorescence reading of about 11,000 units. This response is inhibited with increasing concentrations of STI, as predicted by known trypsin inhibitors. Ugly succession dilutions were tested in this test and found to inhibit trypsin activity. As shown in Fig. 11, the trypsin inhibitory activity of soybean milk is similar to STI with a purity of about 0.2%. It shows that the hair growth effect of soybean milk itself is at least partly from STI and BBI. Stem-free 7: Signed Stark ^ tyrosinase and TRP-1 protein expression histological analysis of soymilk-treated skin samples described in the above examples showed reduced pigmentation in the hair follicles. To further understand the mechanism of soymilk-induced depigmentation ’we studied the key enzyme tyrosinase and melanin production enzyme tyrosinase-related protein (TRp_ 1). C57B1 / 6 and C3H mice were treated as described above, and samples were collected throughout the study for protein analysis. Use standard procedures, such as the current procedures in Chapter 6: Cell Biology, edited by Juan s. Bonifadno et al. (Copyright 1999: John Wiley & Sons,

Inc·)，進行蛋白質萃取及西方墨潰分析，該文獻整體併 於此以供參考用。如此研究之一範例示於圖12。 -20- 本紙張尺度適用中國國家標準（CNS )八4^洛（210 X297公釐） (請先閱讀背面之注意事項再填寫本頁) •裝· 、·ιτ· 592718 A7 B7 五、發明說明（19 ) 等量之萃取自皮膚的蛋白質以抗酪胺酸酶抗體，，抗 PEP1”及抗-TRP-1抗體”抗PEP7”探測，其述於Jimeneze， Μ” Kameyama，K” Maloy，WL，Tomita Y·，及 Hearing，V·哺 乳動物酪胺酸酶：生合成、處理及藉由黑色素細胞刺激荷 爾蒙之調節，Proc· Natl. Acad. Sci· USA (1988)，85:3830-34 及 Jimenez，Μ” K” Maloy，WL，及 Hearing，V·確實之路胺 酸酶純系之專一性鑑定，J· Biol. Chem. (1989) 264 : 3397_ 34〇3，其整體併於此以供參考用。 如圖12所示，酿胺酸酶及TRP-1蛋白質之表現顯著 地由豆漿處理所影響。酪胺酸酶及TRP-1之量減少，及表 現之持續期間縮短。此二因素影響整體毛髮之色素沉著， 其因黑色素生成之量減少及持續期間較短而減少。 ΪΜΑ:豆漿減少人類臉部毛髮之長度及晟唐 每天刮毛之具深色臉部毛髮之男性個人在刮毛後立即 以豆漿處理其臉右側長達5週。在第3週前及更顯著地在 第4週前，經處理之該側毛髮可看出較淡且觸摸感覺較柔 順。處理期間使用Hi-Scope在不同放大倍數下拍攝數位 照片。此等照>{清楚地證明處理側中毛幹尺寸及厚度之減 小。如此照片之範例示於圖13，證明四週處理後，毛幹 厚度及密度之差異。因臉部兩側均在同一時間刮毛且兩側 照片係在同一時間拍攝，臉部毛髮長度之差異顯示在處理 區域中較緩慢之生長速度。 圖14顯示豆漿處理四週後臉部毛髮長度、厚度及全 -21- 本紙張尺度適財國國家標準（CNS)A4規格（210 X 297公髮) Γ%先閱讀背面之注意事項再填寫本頁} I I- I I ^ ·1111111· m 經濟部智慧財產局員工消費合作社印製 592718 A7 B7 五、發明說明（ 20 經濟部智慧財產局員工消費合作社印製 區之電腦化影像分析。所有影像以Image Pro Plus 3.0軟體 (Media Cybernetics，Silver Spring, MD)分析。數據以臉部各 側180根毛幹之平均值與標準誤差呈現(SigmaPi〇t®5.0, SPSS Science，Chicago，IL)。使用 SigmaStat®2.0 (SPSS Science)軟體進行統計分析，證明豆漿處理後所有測量之 參數中均有統計上顯著的差異。 實例9 :豆漿減少人類腳部毛髮長度及厚度 一個人膝蓋下方腳中間部份之兩對稱區以壤除毛。每 天以豆漿處理一腳長達4週。視覺觀察顯示處理部位之毛 髮生長較慢。毛幹之數目較未處理部位者少且較短及較稀 疏，如圖15所示。此等觀察進一步確認豆漿對毛髮生長 之效果。實例8及9 一起確認豆漿對人類毛髮生長之效果 與除毛方法或被處理之身體部位無關。 寬全!一111>入異黃酮之豆漿配方於降低革▲髮生長及色免 沉著上較豆漿配方佳 使用表2所述之兩個配方：豆聚為基質之配方豆聚精 23及與豆漿精23相同但添加5%之〇1%異黃酮萃取物之 豆漿精30重覆實例4所述之實驗。如圖16所示，以大豆 精23處理之鼠顯現較少之毛髮生長及較佳之毛髮外觀。 此效果以使用豆漿精30者較顯著，證明添加異黃綱之豆 黎配方在減少毛髮生長上優於豆聚配方。圖η顯示在處 理第15天時，經處理之鼠的組織皮膚斷面。此等斷面所 -22- L紙張尺度適用中國國家標準（CNS)A4規格⑵〇 χ 297公^~ 裝—— (請先閱讀背面之注意事項再填寫本頁) 訂·- -I I I - 592718 A7 B7Inc.) for protein extraction and Western blot analysis, the entire document is hereby incorporated by reference. An example of such a study is shown in Figure 12. -20- This paper size applies to Chinese National Standards (CNS) 8 4 ^ Luo (210 X297 mm) (Please read the precautions on the back before filling this page) • Installation · ·· ιτ · 592718 A7 B7 V. Description of the invention (19) Equal amounts of skin-extracted proteins are detected with anti-tyrosinase antibodies, anti-PEP1 "and anti-TRP-1 antibodies" anti-PEP7 ", as described in Jimeneze, M" Kameyama, K "Maloy, WL , Tomita Y ·, and Hearing, V · Mammalian tyrosinase: Biosynthesis, processing, and hormone regulation by melanocyte stimulation, Proc. Natl. Acad. Sci · USA (1988), 85: 3830-34 and Jimenez, M ”K” Maloy, WL, and Hearing, V. Specific identification of pure ureases, J. Biol. Chem. (1989) 264: 3397_34〇3, the entirety of which is hereby provided for For reference. As shown in Figure 12, the performance of glutamidase and TRP-1 protein is significantly affected by soymilk treatment. The amount of tyrosinase and TRP-1 is reduced, and the duration of performance is shortened. These two factors Pigmentation that affects the overall hair, which is reduced due to a reduction in the amount of melanin produced and a shorter duration ΪΜΑ: Soy milk reduces the length of human facial hair and males with dark facial hair shaved every day by Shengtang. After shaving, the right side of their face is treated with soy milk for up to 5 weeks. Before 3 weeks and more Significantly before the 4th week, the treated side of the hair can be seen to be lighter and softer to the touch. During the treatment, Hi-Scope was used to take digital photos at different magnifications. These photos > {clearly prove the treatment The size and thickness of the hair shaft in the side are reduced. An example of this photo is shown in Figure 13, which proves the difference in thickness and density of hair shaft after four weeks of treatment. Because both sides of the face are shaved at the same time and the photos on both sides are in At the same time, the difference in the length of facial hair shows a slower growth rate in the treated area. Figure 14 shows the length, thickness, and total of facial hair after soymilk treatment for four weeks. A4 specifications (210 X 297) Γ% Please read the notes on the back before filling in this page} I I- II ^ · 1111111 · m Printed by the Consumers ’Cooperative of the Intellectual Property Bureau of the Ministry of Economy 592718 A7 B7 V. Description of the invention (20 Ministry of Economic Affairs Computerized image analysis of the printed area of the Consumer Cooperative of the Intellectual Property Bureau. All images were analyzed with Image Pro Plus 3.0 software (Media Cybernetics, Silver Spring, MD). Data are presented as the average and standard error of 180 hair shafts on each side of the face (SigmaPilot® 5.0, SPSS Science, Chicago, IL). Statistical analysis was performed using SigmaStat® 2.0 (SPSS Science) software, which proved that there were statistically significant differences in all measured parameters after soymilk treatment. Example 9: Soymilk reduces hair length and thickness of human feet. Two symmetrical areas of the middle part of the foot under the knee of a person remove hair. Treat your feet with soy milk daily for up to 4 weeks. Visual observation showed that hair growth was slower in the treated area. The number of hair shafts is smaller, shorter and thinner than those of untreated areas, as shown in Fig. 15. These observations further confirmed the effect of soybean milk on hair growth. Examples 8 and 9 together confirmed that the effect of soy milk on human hair growth has nothing to do with the method of hair removal or the body parts being treated. Kuanquan! -111> Isoflavone-containing soymilk formula is used to reduce leather. ▲ Growth and color are better than soymilk formula. Use the two formulas described in Table 2: Soybean concentrate 23 and Soymilk. The soymilk extract 30 with the same essence 23 but with 5% isoflavone extract 30% was added to repeat the experiment described in Example 4. As shown in Fig. 16, rats treated with soybean extract 23 showed less hair growth and better hair appearance. This effect is more pronounced with those using soymilk extract 30, which proves that the Dou Li formula added with Isoxan is better than the Dou Poly formula in reducing hair growth. Figure n shows a section of the tissue skin of the treated mice on day 15 of treatment. The paper size of these sections is -22-L paper size applicable to the Chinese National Standard (CNS) A4 specification 〇〇χ 297 公 ^ ~ Pack-(Please read the precautions on the back before filling this page) Order---III-592718 A7 B7

I 訂 LI order L

鞮 592718 A7 經濟部智慧財產局員工消費合作社印製 五、發明說明（22 調查者評估、試驗者自麟估及Hi_seGPe f彡像分析檢視。 試驗者簽署被告知同意書並指示其研究程序及期望與要求 當晚刮毛。第二天基線(baseline)探視時，分給各試驗者二 個試驗化粧水（第1天）：安慰劑化粧水及豆漿精23或 30。化粧水隨機給予右腳或左腳。在研究期間不使用其他 化粧水於腳部下方，將試驗化链水及安慰劑用於各腳。試 驗者經指示每日施予試驗化粧水早晚兩次並被指示嘗試不 到除腳#下方之毛髮直至各週評估後。評估當天，調查者 以視見檢查試驗者腳部是否有任何臨床刺激徵狀及比較腳 部之毛髮生長變稀疏之程度。在各評估時間點（第卜2、 3點i用4 2由試驗者完成自我評估問卷。此外，在各時間 ^ _MACR0Z 鏡頭(Hirox)取得 Hi-scope 影像（各 影像f徑^公分，咖微，Hirox)。P〜像（各 在驗者係因任何產品相關之理由自研究退出。未 究期間H點在任一研究試驗者看到刺激徵狀，亦未在研 刺激。B壬_時間點上於試驗者所作自我評估中報告有任何 驗者财毛1數目，在各時_各試 在第5遇〜A ” 域之計數。結果顯示對於轉精30， 週則及對於豆漿精23 減少。雖然掉準誤第週刖腳部下方毛髮數 中未顯示平约毛古但經安慰劑處理之腳在研究全程 對二各試驗部位使用1，一 之hwcope影像計算得到）除以自最 分析 :後一 (請先閱讀背面之注意事項再填寫本頁) -I I I I I I — — — — — — — — — I1 '1» 卜紙張尺度 -24- 規格（210 X ^97公复) 592718 五、發明說明（23) 次1 毛算起之時間長度（天）而計算得到。結果記錄於表 3 精23及3G處理二者均較安制產生較減緩 之毛髮生長速度。 豆漿精30 ------ 0-211(^007) 〇124^(±〇：〇)5^16(^05) 豆漿精23 -—----- 0^184(^0.07) g：j91(±〇.〇6) ^153(士 〇·〇3) 0-213(^0.03) 込181(土〇.〇4) 0.195(±0.〇7) 〇.185(±0·〇9) 安慰劑 〇·243(土 〇·〇7) Qj35(±0.09) Qj^l(±0.06) 〇jj8(±0.07) gj2jj±0.07) 〇·217(±〇.〇9) 〇^36(±〇j^2) 〇·241(±〇·〇6)鞮 592718 A7 Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs. 5. Description of Inventions (22 Investigator's Evaluation, Tester's Self-Assessment, and Hi_seGPe f Image Analysis Review. The tester signed the informed consent and instructed his research procedures and expectations. Shave that night. On the second day of the baseline visit, two trial lotions (day 1) were given to each tester: placebo lotion and soy milk essence 23 or 30. The lotion was randomly given to the right foot or Left foot. During the study period, no other lotion was used under the feet. Tested water and placebo were used for each foot. The tester was instructed to apply the test lotion twice a day and morning and was instructed to try to remove the lotion. The hair under foot # was evaluated after each week. On the day of evaluation, the investigator visually inspected the tester's feet for any signs of clinical irritation and compared the degree of thinning and thinning of the hair on the foot. At each evaluation time point (Art. The self-assessment questionnaire was completed by the tester at 2 and 3 o'clock. In addition, Hi-scope images were obtained at each time ^ _MACR0Z lens (Hirox) (each image f diameter ^ cm, coffee micro, Hirox). P ~ (Each examinee withdrew from the study for any product-related reasons. During the period of no study, the stimulus symptoms were not seen in any of the study testers at point H, and no stimulus was being studied. B Ren_ self-assessment made by the tester at the time point In the report, there is a count of any examinee's wealth 1. At each time _ each test counts in the 5th encounter ~ A ”field. The results show that for the conversion of 30, the week and the soymilk 23 decrease. The number of hairs below the soles of the foot did not show flat hair, but the placebo-treated feet were divided into two test sites by using 1, and the hwcope image was calculated during the entire study period. Divided by the most recent analysis: the last one (please read first) Note on the back, please fill out this page again) -IIIIII — — — — — — — — — I1 '1 »Bu paper scale-24- Specifications (210 X ^ 97 public copy) 592718 5. Description of the invention (23) Times 1 gross The calculated length of time (days) is calculated. The results are recorded in Table 3. Both fine 23 and 3G treatments produce slower hair growth rate than the system. Soymilk 30 ------ 0-211 (^ 007) 〇124 ^ (± 〇: 〇) 5 ^ 16 (^ 05) Soymilk extract 23 --------- 0 ^ 184 (^ 0.07) g : J91 (± 0.06) ^ 153 (± 0.03) 0-213 (^ 0.03) 込 181 (± 0.04) 0.195 (± 0.07) 〇.185 (± 0 · 〇) 9) Placebo 〇243 (Tou 〇07) Qj35 (± 0.09) Qj ^ l (± 0.06) 〇jj8 (± 0.07) gj2jj ± 0.07) 〇217 (± 〇.〇9) 〇 ^ 36 ( ± 〇j ^ 2) 〇241 (± 〇 · 〇6)

下方 〇·194(±〇·〇5) 0.208(土 0·07) 0.220(士 〇·〇4) (請先M讀背面之注咅？事項再填寫本頁) 裝----------訂--------- _ 經濟部智慧財產局員工消費合作社印製 自=驗者自我砰估問卷之結果顯示試驗者感覺試驗化 粧水她於安慰劑化粧水減少毛髮之生長及軟化腳 觸覺。試驗者«毛髮較不粗糙且短毛較少。大部份之試 驗者相信試驗化ϋ水減少腳毛髮之生長或改變毛髮:紋二 以致感覺«順及較不贿。Hi SeGpe分析進—步證明以 豆漿精23或3G處理後毛髮似乎以相同方向再生=且妒 -25- 「紙張尺度適用中關家標準（CNS)A4規袼咖x 297公楚丁 592718 A7 B7_ 五、發明說明（24) 狀、紋理及外觀更均勻。相反地，在經安慰劑處理之腳上 再生長之毛髮以不同方向、不同長度、不同生長角度及厚 度生長。 此實例清楚地證明豆漿配方於延缓及減少毛髮生長並 使毛髮較柔軟、較不粗糙及更易處理與定向生長之效果。 裳—— (請先、W'讀背面之注意事項再填寫本頁) 訂· ¼. 經濟部智慧財產局員工消費合作社印製 -26- 本紙張尺度適用中國國家標準（CNS)A4規格（210 X 297公釐）Bottom 〇 · 194 (± 〇 · 〇5) 0.208 (土 0 · 07) 0.220 (士 〇 · 〇4) (please read the note on the back first? Matters before filling out this page) Installation ------- --- Order --------- _ Printed by the Consumers' Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs. The result of the self-evaluation questionnaire showed that the tester felt that the test lotion reduced her hair in the placebo lotion. Grow and soften the feel of your feet. Subject «hair is less rough and has less short hair. Most of the testees believe that the test of hydrating water can reduce the growth of the hair on the feet or change the hair: Line 2 so that it feels «come and less bribe. Hi SeGpe analysis further proves that hair treated with soymilk extract 23 or 3G seems to regenerate in the same direction = and jealous -25-"The paper size applies the Zhongguanjia Standard (CNS) A4 Regulations Coffee x 297 Gong Chuding 592718 A7 B7_ 5. Description of the invention (24) The shape, texture and appearance are more uniform. On the contrary, the hair regrown on the placebo-treated feet grows in different directions, different lengths, different growth angles and thicknesses. This example clearly proves soy milk The formula delays and reduces hair growth and makes the hair softer, less rough, and easier to handle and directional growth. Sang-(please read the precautions on the back before filling this page) Order ¼. Ministry of Economic Affairs Printed by the Intellectual Property Bureau's Consumer Cooperatives-26- This paper size applies to China National Standard (CNS) A4 (210 X 297 mm)

Claims (1)

592718592718 申請專科n 專利申請案第89114979號 ROC Patent Appln. No.89114979 >正後無劃線之申請專利範圍中文本—附件(二） Amended Claims in Chinese ~ Enel.(Ή) (民國93年4月巧 曰送呈） (Submitted on April , 2004) 2· 4· 經濟部智慧財產局員Η消費合作、社印製 6. 一種改變哺乳動物毛髮外觀、毛髮生長、毛髮色素沉 著及毛囊與毛幹尺寸之組成物，其包含局部施予有效 S之局部活性組成物，該組成物係包含豆科植物之萃 取物或自其衍生之絲胺酸蛋白酶抑制劑。 根據申請專利範圍第1項之組成物，其中該絲胺酸蛋 白酶抑制劑之存在量為0.0001% (重量/體積）至20% (重量/體積），以局部活性組成物之總體積為基準。 根據申請專利範圍第2項之組成物，其中該絲胺酸蛋 白酶抑制劑之存在量為0.001% (重量/體積)至5% (重 量/體積），以局部活性組成物之總體積為基準。 板據申請專利範圍第1項之組成物，其中該組成物進 —步包含&或多種異黃酮。 根據申請專利範圍第1項之組成物，其中該組成物進 ~步包括一或多種含一或多種異黃酮之天然萃取物。 根據申請專利範圍第1項之組成物’其中該組成物進 步包含界面活性劑、濕潤劑、保濕劑、發泡劑、化 粧品賦形劑、緩衝劑、保藏劑、抗氧化劑、調理劑、 去色素劑、除毛劑、抗老化劑、防晒劑、香料、著色 劑或其混合物。 根據申請專利範圍第1項之組成物，其中該組成物呈 液體、乳霜、凝膠、糊狀、粉末、精油、慕斯、乳 27 - 本紙張尺产^ 一 "—— ^ 中國國家標準(°^)八4規格（210x297公釐） 89395Β-接 592718 A8 B8 C8 D8 六、申請專利範圍 液、膜貼片或喷霧劑形式。 8. 根據申請專利範圍第1項之組成物，其中該組成物衍 生自莢果。 9. 根據申請專利範圍第8項之組成物，其中該組成物衍 生自大豆、萊豆及/或黑豆。 10. 根據申請專利範圍第9項之組成物，其中該組成物選 自包含豆漿、萊豆襞、黑豆聚、大豆萃取物、萊豆萃 取物、黑豆萃取物、大豆糊、萊豆糊、黑豆糊、大豆 粉、黑豆粉、萊豆粉、豆漿粉、黑豆漿粉及萊豆漿粉 與其混合物。 11. 根據申請專利範圍第10項之組成物，其中該組成物係 豆漿、大豆萃取物、大豆糊、萊豆漿、萊豆萃取物、 萊豆糊、黑豆漿、黑豆萃取物、黑豆糊、大豆粉、黑 豆粉、萊豆粉、豆漿粉、黑豆漿粉及萊豆漿粉與其混 合物之部份。 12. 根據申請專利範圍第10項之組成物，其包含1至99% 重量比之豆漿。 經濟部智慧財產局員工消費合作社印製 13. 根據申請專利範圍第10項之組成物，其包含1至99% 重量比之大豆粉、豆漿粉或其混合物。 14. 根據申請專利範圍第11項之組成物，其包含0.0001至 20%重量比之大豆胰蛋白酶抑制劑、萊豆胰蛋白酶抑 制劑或黑豆蛋白酶抑制劑。 15. 根據申請專利範圍第8項之組成物，其中該組成物進 一步包含一或多種異黃酮。 -28 - 本紙張尺度適用中國國家標準(CNS)A4規格（210x297公釐） 592718 六 經濟部智慧財產局員工消費合作社印製 Α8 Β8 C8 D8 申請專利範圍 16. 根據申請專利範圍第8項之組成物，其中該組成物進 一步包括一或多種含一或多種異黃酮之天然萃取物。 17. 根據申請專利範圍第15項之組成物，其中該一或多種 異黃酮佔該組成物之0.00001至0.1%重量比。 18. 根據申請專利範圍第16項之組成物，其中該一或多種 含一或多種異黃酮之天然萃取物佔該組成物之0.00001 至0.1%重量比。 19. 根據申請專利範圍第1項之化粧品組成物，其中該組 成物進一步包括化粧品可接受之賦形劑。 20. 根據申請專利範圍第1項之組成物，其中該組成物進 一步包含脂質體。 21. 根據申請專利範圍第20項之組成物，其中該組成物包 含甘油二月桂酸酯、膽固醇、聚氧伸乙基-10-硬脂醯基 鍵及聚氧伸乙基-9-月桂基鍵。 22. 根據申請專利範圍第1項之組成物，其中該組成物包 含1至99%豆漿、0.1%至20%乳化劑及有效量之保藏 劑。 23. 根據申請專利範圍第1項之組成物，其中該組成物包 含0·5至20%豆漿粉、0.1%至20%乳化劑及有效量之 保藏劑。 24. 根據申請專利範圍第22項之組成物，其中該組成物進 一步包含0.00001至0.1%之一或多種的異黃酮。 25. 根據申請專利範圍第22項之組成物，其中該組成物進 一步包含0.0001至0.1%之一或多種含一或多種異黃酮 -29 - 本紙張尺度適用中國國家標準(CNS)A4規格（210x297公釐） 592718 A8 B8 C8 _D8_ 六、申請專利範圍 之天然萃取物。 26. 根據申請專利範圍第1項之組成物，其中該組成物進 一步包含0.0001至0.1%之一或多種的異黃酮。 27. 根據申請專利範圍第23項之組成物，其中該組成物進 一步包含0.0005至0.05%之一或多種含一或多種異黃 酮之天然萃取物。 經濟部智慧財產局員工消費合作社印製 本紙張尺度適用中國國家標準(CNS)A4規格（210 X 297公釐）Applying for Specialties n Patent Application No. 89114979 ROC Patent Appln. No. 89114979 > Scope of Patent Application without Front and Back Chinese Text—Annex (II) Amended Claims in Chinese ~ Enel. (Ή) (April 1993) (Submitted on April, 2004) 2 · 4 · Member of the Intellectual Property Bureau of the Ministry of Economic Affairs, Consumer Cooperative, Printed by the Society 6. A composition that changes mammalian hair appearance, hair growth, hair pigmentation, and hair follicle and hair shaft size A substance comprising a topically active composition for topical administration of effective S, the composition comprising an extract of a leguminous plant or a serine protease inhibitor derived therefrom. The composition according to item 1 of the scope of patent application, wherein the serine protease inhibitor is present in an amount of 0.0001% (weight / volume) to 20% (weight / volume), based on the total volume of the topically active composition. The composition according to item 2 of the scope of patent application, wherein the serine protease inhibitor is present in an amount of 0.001% (weight / volume) to 5% (weight / volume), based on the total volume of the topically active composition. The composition according to item 1 of the scope of patent application, wherein the composition further comprises & or more isoflavones. The composition according to item 1 of the patent application scope, wherein the composition further comprises one or more natural extracts containing one or more isoflavones. The composition according to item 1 of the scope of the patent application, wherein the composition progress includes a surfactant, a humectant, a humectant, a foaming agent, a cosmetic excipient, a buffer, a preservative, an antioxidant, a conditioner, and a depigmentation agent. Agents, hair removers, anti-aging agents, sunscreens, perfumes, colorants or mixtures thereof. The composition according to item 1 of the scope of the patent application, wherein the composition is liquid, cream, gel, paste, powder, essential oil, mousse, milk 27-produced by this paper rule ^ 一 " —— ^ China Standard (° ^) 8 4 specifications (210x297 mm) 89395B-then 592718 A8 B8 C8 D8 VI. Patent application scope Liquid, film patch or spray form. 8. The composition according to item 1 of the scope of patent application, wherein the composition is derived from a pod. 9. The composition according to item 8 of the scope of patent application, wherein the composition is derived from soybeans, lentils, and / or black beans. 10. The composition according to item 9 of the scope of the patent application, wherein the composition is selected from the group consisting of soybean milk, lentil, black bean poly, soy extract, lentil extract, black bean extract, soybean paste, lentil paste, black beans Paste, soybean flour, black soybean flour, lentils flour, soybean milk powder, black soybean milk powder, and soybean milk powder and mixtures thereof. 11. The composition according to item 10 of the scope of patent application, wherein the composition is soybean milk, soybean extract, soybean paste, lentils milk, lentils extract, lentils paste, black soybean milk, black bean extract, black bean paste, soybeans Powder, black soybean flour, lentils flour, soybean milk powder, black soybean milk powder, and portions of raisin powder and mixtures thereof. 12. The composition according to item 10 of the patent application scope, which comprises 1 to 99% by weight of soybean milk. Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs 13. The composition according to item 10 of the scope of patent application, which contains 1 to 99% by weight of soybean flour, soybean milk flour or mixtures thereof. 14. A composition according to item 11 of the scope of patent application, which comprises 0.0001 to 20% by weight of a soybean trypsin inhibitor, a lentil trypsin inhibitor or a black bean protease inhibitor. 15. A composition according to item 8 of the patent application scope, wherein the composition further comprises one or more isoflavones. -28-This paper size applies the Chinese National Standard (CNS) A4 specification (210x297 mm) 592718 Printed by the Consumers ’Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs A8 Β8 C8 D8 Scope of patent application 16. Composition according to item 8 of the scope of patent application Wherein the composition further comprises one or more natural extracts containing one or more isoflavones. 17. The composition according to item 15 of the scope of patent application, wherein the one or more isoflavones account for 0.00001 to 0.1% by weight of the composition. 18. The composition according to item 16 of the scope of patent application, wherein the one or more natural extracts containing one or more isoflavones account for 0.00001 to 0.1% by weight of the composition. 19. The cosmetic composition according to item 1 of the scope of patent application, wherein the composition further includes an cosmetically acceptable excipient. 20. The composition according to item 1 of the patent application scope, wherein the composition further comprises liposomes. 21. The composition according to item 20 of the scope of patent application, wherein the composition comprises glyceryl dilaurate, cholesterol, polyoxyethyl-10-stearyl bond, and polyoxyethyl-9-lauryl key. 22. The composition according to item 1 of the scope of patent application, wherein the composition comprises 1 to 99% of soybean milk, 0.1% to 20% of an emulsifier, and an effective amount of a preservative. 23. The composition according to item 1 of the scope of patent application, wherein the composition contains 0.5 to 20% of soybean milk powder, 0.1% to 20% of an emulsifier, and an effective amount of a preservative. 24. The composition according to item 22 of the scope of patent application, wherein the composition further contains one or more isoflavones of 0.00001 to 0.1%. 25. The composition according to item 22 of the scope of patent application, wherein the composition further contains one or more kinds containing one or more isoflavones of 0.0001 to 0.1%-this paper size applies the Chinese National Standard (CNS) A4 specification (210x297) Mm) 592718 A8 B8 C8 _D8_ VI. Natural extracts with patent application scope. 26. The composition according to item 1 of the patent application scope, wherein the composition further contains one or more isoflavones at 0.0001 to 0.1%. 27. The composition according to item 23 of the patent application scope, wherein the composition further comprises 0.0005 to 0.05% of one or more natural extracts containing one or more isoflavones. Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs This paper is sized for China National Standard (CNS) A4 (210 X 297 mm)