TW258753B - Direct fermentation of L-tryptophan by a recombinant microbe - Google Patents

Direct fermentation of L-tryptophan by a recombinant microbe

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Publication number
TW258753B
TW258753B TW81105135A TW81105135A TW258753B TW 258753 B TW258753 B TW 258753B TW 81105135 A TW81105135 A TW 81105135A TW 81105135 A TW81105135 A TW 81105135A TW 258753 B TW258753 B TW 258753B
Authority
TW
Taiwan
Prior art keywords
tryptophan
ems4
coli
strain
produce
Prior art date
Application number
TW81105135A
Other languages
Chinese (zh)
Inventor
Shiow-Lan Tsay
Eel-Chang Jan
Duey-Gang Mou
Original Assignee
Dev Center Biotechnology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Dev Center Biotechnology filed Critical Dev Center Biotechnology
Priority to TW81105135A priority Critical patent/TW258753B/en
Application granted granted Critical
Publication of TW258753B publication Critical patent/TW258753B/en

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Classical mutagenesis could desensitize the feedback inhibition of L-tryptopHan biosynthesis. Among the mutants, Escherichia coli EMS4 produced 3 g/1 of L-tryptopHan within 18 hours. The feedback-resistant L-Trp operon gene prepared from E. coli EMS4 was inserted into pUC19 and pHSG576 to generate pTC701 and pTC576. When these plasmids were introduced into E. coli EMS4, chromosomal integration occurred through homologous recombination. By using Southern hybridization, we demonstrated that the integrated plasmids existed as multicopies. The strains with integrated foreign trp operon gene could produce 5.5 g/1/18h of L-tryptopHan, and this strain was named as E. coli EMS4 C25. This strain was very stable in fermentation process, even in the absance of antibiotics it could produce a high yield of L-tryptopHan.
TW81105135A 1992-06-30 1992-06-30 Direct fermentation of L-tryptophan by a recombinant microbe TW258753B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
TW81105135A TW258753B (en) 1992-06-30 1992-06-30 Direct fermentation of L-tryptophan by a recombinant microbe

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
TW81105135A TW258753B (en) 1992-06-30 1992-06-30 Direct fermentation of L-tryptophan by a recombinant microbe

Publications (1)

Publication Number Publication Date
TW258753B true TW258753B (en) 1995-10-01

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ID=51401775

Family Applications (1)

Application Number Title Priority Date Filing Date
TW81105135A TW258753B (en) 1992-06-30 1992-06-30 Direct fermentation of L-tryptophan by a recombinant microbe

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Country Link
TW (1) TW258753B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998004715A1 (en) * 1996-07-30 1998-02-05 Archer-Daniels-Midland Company Novel strains of escherichia coli, methods of preparing the same and use thereof in fermentation processes for l-threonine production
EP0789073A3 (en) * 1995-09-05 1998-09-23 Degussa Aktiengesellschaft Production of tryptophan by the bacterium Escherichia coli

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0789073A3 (en) * 1995-09-05 1998-09-23 Degussa Aktiengesellschaft Production of tryptophan by the bacterium Escherichia coli
WO1998004715A1 (en) * 1996-07-30 1998-02-05 Archer-Daniels-Midland Company Novel strains of escherichia coli, methods of preparing the same and use thereof in fermentation processes for l-threonine production
US5939307A (en) * 1996-07-30 1999-08-17 The Archer-Daniels-Midland Company Strains of Escherichia coli, methods of preparing the same and use thereof in fermentation processes for l-threonine production

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PHOSPHORYLASE Moscow GENETIKA in Russian Vol 19, No 6, Jun 83 (manuscript received 16 Sep 82) pp 881-887 MAZNITSA, II, SUKHODOLETS, VV and UKHABOTINA, LS, All-Union Scientific Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow 113545