TW202420995A - Plant resistance enhancer with long-term sustainable action - Google Patents

Abstract

An object of the present invention is to provide a resistance enhancer for continuously increasing or improving heat resistance and drying resistance of plants for a long period of time. The present invention relates to a resistance enhancer for continuously increasing or improving heat resistance and drying resistance of plants for 2 months or more, the resistance enhancer contains at least one unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal, which is at least one selected from the group consisting of α,β-alkenal, α,β-alkenone, γ,δ-alkenal, γ,δ-alkenone, δ,ε-alkenal, δ,ε-alkenone, ε,δ-alkenal, and ε,δ-alkenone; and a triethyl citrate. The present invention also relates to a device for generating the resistance enhancer.

Description

具有長期持續作用之植物耐受性增強劑 Plant tolerance enhancer with long-lasting effect

本發明係有關可長期持續增強或改善植物的耐熱性及耐乾燥性用之耐受性增強劑。此外，本發明也有關產生該耐受性增強劑的產生器。 The present invention relates to a tolerance enhancer that can enhance or improve the heat resistance and desiccation resistance of plants in a long-term and continuous manner. In addition, the present invention also relates to a generator for producing the tolerance enhancer.

由於近年來的全球氣候暖化，夏季的高溫危害已成為農業領域的一大難題。以往，為了防止高溫損害，雖然已進行在戶外安裝遮陽傘或溫室內安裝換氣扇的設施，但因需要大額資金而不易實施。因此，期望開發在許多農業現場上容易使用的植物之耐高溫性誘發劑。 Due to global warming in recent years, the damage caused by high temperatures in summer has become a major problem in the agricultural field. In the past, in order to prevent damage from high temperatures, facilities such as installing sunshades outdoors or installing ventilation fans in greenhouses have been carried out, but they are difficult to implement because they require a large amount of funds. Therefore, it is hoped that a plant high temperature resistance inducer that can be easily used in many agricultural fields will be developed.

此種情況下，已有如乙基乙烯酮或羥基丙烯醛的特定化合物可誘發植物的高溫耐受性之報告(專利文獻1)。此外，選自特定不飽和醛及不飽和酮中的至少一種不飽和羰基化合物不僅具有耐熱性(耐高溫性)及耐乾燥性，也可增強或改善其他耐受性(耐損傷性、耐光性等)之報告(專利文獻2)。由於此等耐受性增強劑即使無基因改造也可改善或增強耐熱性、耐乾燥性等耐受性，因此可望使用於禁止種植基因改造作物的國家，或對傳統品種有效。 In this case, there are reports that specific compounds such as ethyl ethylene ketone or hydroxyacrolein can induce high temperature tolerance in plants (Patent Document 1). In addition, there are reports that at least one unsaturated carbonyl compound selected from specific unsaturated aldehydes and unsaturated ketones not only has heat resistance (high temperature resistance) and desiccation resistance, but can also enhance or improve other tolerances (damage resistance, light resistance, etc.) (Patent Document 2). Since these tolerance enhancers can improve or enhance heat resistance, desiccation resistance, etc. even without genetic modification, they are expected to be used in countries that prohibit the cultivation of genetically modified crops, or be effective for traditional varieties.

雖然已確認上述耐受性增強劑的耐受性增強效果可持續大約2至3週(1個月內)，但有效成分化合物在分子結構上容易因氧化而變質，非常不穩 定，故尚未有可整個季節(夏季兩個月或更長時間)使用的耐受性增強劑之報告例。 Although it has been confirmed that the tolerance-enhancing effect of the above-mentioned tolerance enhancers can last for about 2 to 3 weeks (within 1 month), the active ingredient compounds are easily deteriorated by oxidation in molecular structure and are very unstable. Therefore, there are no reports of tolerance enhancers that can be used throughout the season (two months or longer in summer).

在農業領域中，在改善作業的便利性之期望中，強烈渴望開發出不需頻繁更換、具有長期持續作用的植物之耐受性增強劑。 In the agricultural field, in the hope of improving the convenience of work, there is a strong desire to develop plant tolerance enhancers that do not require frequent replacement and have long-lasting effects.

[先前技術文獻] [Prior Art Literature]

[專利文獻] [Patent Literature]

[專利文獻1]日本特開2011-157307號公報 [Patent Document 1] Japanese Patent Publication No. 2011-157307

[專利文獻2]國際公開第2016/031775號 [Patent Document 2] International Publication No. 2016/031775

本發明的目的係提供可長期(2個月以上)持續增強或改善植物的耐熱性及耐乾燥性用之耐受性增強劑。 The purpose of the present invention is to provide a tolerance enhancer that can continuously enhance or improve the heat resistance and drought resistance of plants for a long period of time (more than 2 months).

本發明人為解決此問題而深入研究的結果，首次發現一種植物的耐受性增強劑，其係使用選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的不飽和碳數4至9之羰基化合物作為有效成分，且含有檸檬酸三乙酯，藉此可持續2個月以上增強或改善植物的耐熱性及耐乾燥性，同時發現有效產生該耐受性增強劑的方法及該耐受性增強劑的產生器，遂完成本發明。 As a result of in-depth research to solve this problem, the inventors of the present invention have discovered for the first time a plant tolerance enhancer, which uses as an active ingredient at least one unsaturated carbonyl compound with 4 to 9 carbonyl compounds having no unsaturated bond at the end selected from the group consisting of α , β -enal, α , β -enone, γ , δ-enal, γ , δ -enone, δ , ε-enal, δ , ε -enone, ε, ζ-enal and ε , ζ -enone, and contains triethyl citrate, thereby being able to continuously enhance or improve the heat resistance and desiccation resistance of plants for more than 2 months. At the same time, a method for effectively producing the tolerance enhancer and a generator of the tolerance enhancer were discovered, thereby completing the present invention.

即，本發明係如下述。 That is, the present invention is as follows.

〔1〕一種耐受性增強劑(以下，亦稱「本發明的耐受性增強劑」)，係用以持續2個月以上增強或改善植物的耐熱性及耐乾燥性者，其含有選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物、及檸檬酸三乙酯。 [1] A tolerance enhancer (hereinafter also referred to as "the tolerance enhancer of the present invention") is used to enhance or improve the heat resistance and desiccation resistance of plants for more than 2 months, comprising at least one unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal selected from the group consisting of α , β -enal, α , β -enone, γ , δ -enal, γ , δ -enone, δ , ε - enal , δ, ε-enone, ε , ζ -enal and ε , ζ -enone, and triethyl citrate.

〔2〕如上述〔1〕所述之耐受性增強劑，其中，末端不具有不飽和鍵之碳數4至9的不飽和羧基化合物係選自由α,β-烯醛、γ,δ-烯醛、δ,ε-烯醛及ε,ζ-烯醛所組成之群組中的至少1種。 [2] The tolerance enhancer as described in [1] above, wherein the unsaturated carboxyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal is at least one selected from the group consisting of α , β -enal, γ , δ -enal, δ , ε -enal and ε , ζ -enal.

〔3〕如上述〔1〕所述之耐受性增強劑，其中，末端不具有不飽和鍵之碳數4至9的不飽和羧基化合物為α,β-烯醛。 [3] The tolerance enhancer as described in [1] above, wherein the unsaturated carboxyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal is α , β -enal.

〔4〕一種持續2個月以上增強或改善植物之耐熱性及耐乾燥性的方法(以下，亦稱「本發明的耐受性增強方法」)，其包括：(1)將選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物和檸檬酸三乙酯混合，而調製含有該等之組成物(以下，亦稱「本發明的組成物」)的步驟；及(2)將上述末端不具有不飽和鍵的碳數4至9之不飽和羧基化合物釋放至大氣中的步驟。 [4] A method for enhancing or improving the heat and desiccation tolerance of plants for a period of more than two months (hereinafter, also referred to as the "tolerance enhancing method of the present invention"), comprising: (1) introducing a compound selected from the group consisting of α , β -enal, α , β -enone, γ , δ -enal, γ , δ -enone, δ , ε-enal, δ , ε -enone, ε , ζ -enal and ε , ζ -enone into the mixture ; The invention further comprises the steps of: (1) preparing a composition containing at least one unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal of the group consisting of cyclopentane-enone and triethyl citrate; and (2) releasing the unsaturated carboxyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal into the atmosphere.

〔5〕如上述〔4〕所述之方法，其中，末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物係選自由α,β-烯醛、γ,δ-烯醛、δ,ε-烯醛及ε,ζ-烯醛所組成之群組中的至少1種。 [5] The method as described in [4] above, wherein the unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal is at least one selected from the group consisting of α , β -enal, γ , δ -enal, δ , ε -enal and ε , ζ -enal.

〔6〕如上述〔4〕所述之方法，其中，末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物為α,β-烯醛。 [6] The method as described in [4] above, wherein the unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal is α , β -enal.

〔7〕如上述〔4〕至〔6〕中任一項所述之方法，係藉由使用耐受性增強劑的產生器，並將前述可撓性容器折彎，使易碎性容器破裂而進行前述(1)及(2)的步驟，其中，在可設置一個以上的散發孔之可撓性容器內收納至少一個易碎性容器，該易碎性容器係含有選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物，前述可撓性容器中具有散發孔時，將散發孔密封，無散發孔時，則在末端配置吸收性材料，且在易碎性容器的外側且可撓性容器內收納含有檸檬酸三乙酯的液劑。 [7] The method as described in any one of the above [4] to [6] is performed by using a generator of a tolerance enhancer and bending the above flexible container to break the fragile container to perform the above steps (1) and (2), wherein at least one fragile container is contained in a flexible container that can be provided with one or more diffusion holes, and the fragile container contains a compound selected from the group consisting of α , β -enal, α , β -enone, γ , δ -enal, γ , δ -enone, δ , ε -enal, δ , ε -enone, ε , ζ -enal and ε , ζ -an unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal of at least one of the groups consisting of alkenones; when the flexible container has diffusion holes, the diffusion holes are sealed; when there are no diffusion holes, an absorbent material is arranged at the terminal; and a liquid containing triethyl citrate is stored outside the frangible container and inside the flexible container.

〔8〕如上述〔4〕至〔6〕中任一項所述之方法，係藉由使用耐受性增強劑的產生器，並將前述可撓性容器折彎，使易碎容器破裂而進行前述(2)的步驟，其中，在可設置一個以上的散發孔之可撓性容器內收納前述(1)的步驟中所得之至少一個易碎性容器，該易碎性容器包含含有選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物和檸檬酸三乙酯之組成物，前述可撓性容器中具有散發孔時，將散發孔密封，無散發孔時，則在末端配置吸收性材料。 [8] The method as described in any one of the above [4] to [6] is performed by using a generator of a tolerance enhancer and bending the above flexible container to break the fragile container to perform the above step (2), wherein at least one fragile container obtained in the above step (1) is contained in a flexible container that can be provided with one or more diffusion holes, and the fragile container contains a compound selected from the group consisting of α , β -enal, α , β -enone, γ , δ -enal, γ , δ -enone, δ , ε -enal, δ , ε -enone, ε , ζ -enal and ε , ζ - A composition of an unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal of at least one of the groups consisting of alkenone and triethyl citrate, wherein when the flexible container has diffusion holes, the diffusion holes are sealed, and when there are no diffusion holes, an absorbent material is arranged at the terminal.

〔9〕如上述〔4〕至〔6〕中任一項所述之方法，係藉由使用耐受性增強劑的產生器，並將前述可撓性容器折彎，使第1易碎性容器及第2易碎性容器破裂而進行前述(1)及(2)的步驟，其特徵為，在可設置一個以上的散發孔之可撓性容器內收納第1易碎性容器，該第1易碎性容器係含有選自由α,β-烯醛、α,β- 烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物，前述可撓性容器中具有散發孔時，將散發孔密封，無散發孔時，則在末端配置吸收性材料，且在第1易碎性容器的外側且可撓性容器內收納含有檸檬酸三乙酯的第2易碎性容器。 [9] The method as described in any one of the above [4] to [6] is performed by using a generator of a tolerance enhancer and bending the above flexible container to break the first and second fragile containers to perform the above steps (1) and (2), wherein the first fragile container is contained in a flexible container that can be provided with one or more diffusion holes, and the first fragile container contains a compound selected from the group consisting of α , β -enal, α , β -enone, γ , δ -enal, γ , δ -enone, δ , ε -enal, δ , ε -enone, ε , ζ -enal and ε , ζ -enone. -an unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal of at least one of the groups consisting of alkenone and ketone; when the flexible container has diffusion holes, the diffusion holes are sealed; when there are no diffusion holes, an absorbent material is arranged at the terminal; and a second frangible container containing triethyl citrate is placed outside the first frangible container and inside the flexible container.

〔10〕如上述〔4〕至〔6〕中任一項所述之方法，係使用用以持續2個月以上增強或改善植物的耐熱性及耐乾燥性用之耐受性增強劑的產生器，並將可撓性容器折彎，使易碎性容器破裂而進行前述(1)及(2)的步驟，其特徵為，耐受性增強劑的產生器具備：頂部密封、底部開放的圓筒狀可撓性容器，***於前述可撓性容器內部的收納空間之含有選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物的至少一個易碎性容器，附加在前述可撓性容器的底部之附件，及安裝在前述附件和前述可撓性容器之間的滲透膜，其中，前述附件具有與前述可撓性容器之內面相對向的側面及與前述可撓性容器之底部相對向的上表面，前述附件在前述表面具有貫穿孔，同時在前述側面具有一個或多個空隙，且在前述易碎性容器的外側且可撓性容器內收納含有檸檬酸三乙酯的液劑。 [10] The method as described in any one of [4] to [6] above, wherein a tolerance enhancer generator is used to enhance or improve the heat resistance and desiccation resistance of plants for more than 2 months, and the flexible container is bent to break the fragile container to perform the steps (1) and (2) above, wherein the tolerance enhancer generator comprises: a cylindrical flexible container with a sealed top and an open bottom, and a storage space inserted into the flexible container containing a compound selected from the group consisting of α , β -enal, α , β -enone, γ , δ -enal, γ , δ -enone, δ , ε -enal, δ , ε -enone, ε , ζ -enal and ε , ζ-enone. At least one frangible container containing at least one unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal of the group consisting of at least one of olefins and ketones, an attachment attached to the bottom of the aforementioned flexible container, and a permeable membrane installed between the aforementioned attachment and the aforementioned flexible container, wherein the aforementioned attachment has a side surface opposite to the inner surface of the aforementioned flexible container and an upper surface opposite to the bottom of the aforementioned flexible container, the aforementioned attachment has a through hole on the aforementioned surface and one or more gaps on the aforementioned side surface, and a liquid containing triethyl citrate is stored outside the aforementioned frangible container and inside the flexible container.

〔11〕如上述〔4〕至〔6〕中任一項所述之方法，係使用用以持續2個月以上增強或改善植物的耐熱性及耐乾燥性用之耐受性增強劑的產生器，並將可撓性容器折彎，使易碎性容器破裂而進行前述(2)的步驟，其中，該耐受性增強劑的產生器具備：頂部密封、底部開放的圓筒狀可撓性容器，***於前述可撓性容器內部的收納空間之前述(1)步驟中所得的含有選自由α,β-烯醛、α,β-烯酮、 γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物和檸檬酸三乙酯之組成物的至少一個易碎性容器，附加在前述可撓性容器的底部之附件，及安裝在前述附件與前述可撓性容器之間的滲透膜，其中，前述附件具有與前述可撓性容器的內面相對向之側面及與前述可撓性容器的底部相對向之上表面，前述附件在前述表面具有貫穿孔，同時在前述側面具有一個或多個空隙。 [11] The method as described in any one of [4] to [6] above, wherein a tolerance enhancer generator for enhancing or improving the heat resistance and desiccation resistance of plants for more than two months is used, and a flexible container is bent to break the fragile container to carry out the step (2) above, wherein the tolerance enhancer generator comprises: a cylindrical flexible container with a sealed top and an open bottom, and the mixture obtained in the step (1) above containing a compound selected from the group consisting of α , β -enal, α , β -enone, γ , δ-enal, γ, δ -enone, δ , ε -enal, δ , ε -enone, ε , ζ -enal and ε , ζ -enal is inserted into the storage space inside the flexible container . At least one frangible container containing at least one unsaturated carbonyl compound with 4 to 9 carbon atoms and no unsaturated bond at the terminal of at least one of the group consisting of cyclopentane-enone and triethyl citrate, an accessory attached to the bottom of the above-mentioned flexible container, and a permeable membrane installed between the above-mentioned accessory and the above-mentioned flexible container, wherein the above-mentioned accessory has a side surface opposite to the inner surface of the above-mentioned flexible container and an upper surface opposite to the bottom of the above-mentioned flexible container, and the above-mentioned accessory has a through hole on the above-mentioned surface, and at the same time has one or more gaps on the above-mentioned side surface.

〔12〕如上述〔4〕至〔6〕中任一項所述之方法，係使用用以持續2個月以上增強或改善植物的耐熱性及耐乾燥性用之耐受性增強劑的產生器，並將可撓性容器折彎，使易碎性容器破裂而進行前述(1)及(2)的步驟，其中，該耐受性增強劑的產生器具備：頂部密封、底部開放的圓筒狀可撓性容器，***於前述可撓性容器內部的收納空間之含有選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物和檸檬酸三乙酯之組成物的第1易碎性容器，附加在前述可撓性容器的底部之附件，及安裝在前述附件與前述可撓性容器之間的滲透膜，其中，前述附件具有與前述可撓性容器的內面相對向之側面及與前述可撓性容器的底部相對向之上表面，前述附件在前述表面具有貫穿孔，同時在前述側面具有一個或多個空隙，且在第1易碎性容器的外側且可撓性容器內收納含有檸檬酸三乙酯的第2易碎性容器。 [12] The method as described in any one of [4] to [6] above, wherein a tolerance enhancer generator is used to enhance or improve the heat resistance and desiccation resistance of plants for more than 2 months, and the flexible container is bent to break the fragile container to perform the steps (1) and (2) above, wherein the tolerance enhancer generator comprises: a cylindrical flexible container with a sealed top and an open bottom, and a storage space inserted into the flexible container containing a compound selected from the group consisting of α , β -enal, α , β -enone, γ , δ -enal, γ , δ -enone, δ , ε -enal, δ , ε -enone, ε , ζ -enal and ε , ζ -enone. -enone, an unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal, and triethyl citrate, a first frangible container containing a composition of at least one of the group consisting of cyclopentane and cyclopentane, an attachment attached to the bottom of the above-mentioned flexible container, and a permeable membrane installed between the above-mentioned attachment and the above-mentioned flexible container, wherein the above-mentioned attachment has a side surface opposite to the inner surface of the above-mentioned flexible container and an upper surface opposite to the bottom of the above-mentioned flexible container, the above-mentioned attachment has a through hole on the above-mentioned surface, and one or more gaps on the above-mentioned side surface, and a second frangible container containing triethyl citrate is accommodated outside the first frangible container and inside the flexible container.

〔13〕一種耐受性增強劑的產生器(以下亦稱「本發明的耐受性增強劑的產生器(1)」，該耐受性增強劑係用以持續2個月以上增強或改善植物的耐熱性及耐乾燥性者，其特徵為，在可設置一個以上的散發孔之可撓性容器內收納至少一 個易碎性容器，該易碎性容器係含有選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物，前述可撓性容器中具有散發孔時，將散發孔密封，或無散發孔時，則在末端配置吸收性材料，且在易碎性容器的外側且可撓性容器內收納含有檸檬酸三乙酯的液劑。 [13] A tolerance enhancer generator (hereinafter also referred to as "the tolerance enhancer generator (1) of the present invention"), the tolerance enhancer is used to enhance or improve the heat resistance and desiccation resistance of plants for more than 2 months, characterized in that at least one fragile container is contained in a flexible container with one or more diffusion holes, and the fragile container contains a compound selected from the group consisting of α , β -enal, α , β -enone, γ , δ -enal, γ , δ -enone, δ , ε -enal, δ , ε -enone, ε , ζ -enal and ε , ζ -an unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal of at least one of the groups consisting of alkenones; when the flexible container has diffusion holes, the diffusion holes are sealed; or when there are no diffusion holes, an absorbent material is arranged at the terminal; and a liquid containing triethyl citrate is stored outside the frangible container and inside the flexible container.

〔14〕一種增強劑的產生器(以下亦稱「本發明的耐受性增強劑的產生器(2)」，該耐受性增強劑係用以持續2個月以上增強或改善植物的耐熱性及耐乾燥性者，其中，在可設置一個以上的散發孔之可撓性容器內收納第1易碎性容器，該第1易碎性容器係含有選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物，前述可撓性容器中具有散發孔時，將散發孔密封，或無散發孔時，則在末端配置吸收性材料，且在第1易碎性容器的外側且可撓性容器內收納含有檸檬酸三乙酯的第2易碎性容器。 [14] A generator of an enhancer (hereinafter also referred to as "the generator of the tolerance enhancer of the present invention (2)"), the tolerance enhancer is used to enhance or improve the heat resistance and desiccation resistance of plants for more than 2 months, wherein a first fragile container is contained in a flexible container that can be provided with one or more diffusion holes, and the first fragile container contains a compound selected from the group consisting of α , β -enal, α , β -enone, γ , δ -enal, γ , δ -enone, δ , ε -enal, δ , ε -enone, ε , ζ -enal and ε , ζ -an unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal of at least one of the group consisting of alkenone and ketone; when the flexible container has diffusion holes, the diffusion holes are sealed; when there are no diffusion holes, an absorbent material is arranged at the terminal; and a second frangible container containing triethyl citrate is accommodated outside the first frangible container and inside the flexible container.

〔15〕一種耐受性增強劑的產生器(以下亦稱「本發明的耐受性增強劑的產生器(2’)」，該耐受性增強劑係用以持續2個月以上增強或改善植物的耐熱性及耐乾燥性者，其中，在可設置一個以上的散發孔之可撓性容器內至少一個易碎性容器，該易碎性容器係含有選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物和檸檬酸三乙酯的組成物，前述可撓性容器中具有散發孔時，將散發孔密封，或無散發孔時，則在末端配置吸收性材料。 [15] A tolerance enhancer generator (hereinafter also referred to as "the tolerance enhancer generator (2') of the present invention"), the tolerance enhancer is used to enhance or improve the heat resistance and desiccation resistance of plants for more than 2 months, wherein at least one fragile container is provided in a flexible container with one or more diffusion holes, and the fragile container contains a compound selected from α , β -enal, α , β -enone, γ , δ -enal, γ , δ -enone, δ , ε -enal, δ , ε -enone, ε , ζ -enal and ε , ζ - A composition of an unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal of at least one of the groups consisting of alkenone and triethyl citrate, wherein when the flexible container has diffusion holes, the diffusion holes are sealed, or when there are no diffusion holes, an absorbent material is arranged at the terminal.

〔16〕一種耐受性增強劑的產生器(以下亦稱「本發明的耐受性增強劑的產生器(3)」，該耐受性增強劑係用以持續2個月以上增強或改善植物的耐熱性及耐乾燥性者，其特徵為，耐受性增強劑的產生器具備：頂部密封、底部開放的圓筒狀可撓性容器，***於前述可撓性容器內部的收納空間之含有選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物，附加在前述可撓性容器的底部之附件，及安裝在前述附件與前述可撓性容器之間的滲透膜，其中，前述附件具有和前述可撓性容器的內面相對向之側面及與前述可撓性容器的底部相對向之上表面，前述附件在前述表面具有貫穿孔，同時在前述側面具有一個或多個空隙。 [16] A tolerance enhancer generator (hereinafter also referred to as "the tolerance enhancer generator (3) of the present invention"), wherein the tolerance enhancer is used to enhance or improve the heat resistance and desiccation resistance of plants for more than 2 months, and wherein the tolerance enhancer generator comprises: a cylindrical flexible container with a sealed top and an open bottom, and a storage space inserted into the flexible container containing a substance selected from the group consisting of α , β -enal, α , β -enone, γ , δ -enal, γ , δ -enone, δ , ε -enal, δ , ε -enone, ε , ζ -enal and ε , ζ-enone . -enone, an unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the end, an attachment attached to the bottom of the flexible container, and a permeable membrane installed between the attachment and the flexible container, wherein the attachment has a side surface opposite to the inner surface of the flexible container and an upper surface opposite to the bottom of the flexible container, and the attachment has a through hole on the surface and one or more gaps on the side surface.

〔17〕一種耐受性增強劑的產生器(以下亦稱「本發明的耐受性增強劑的產生器(4)」，該耐受性增強劑係用以持續2個月以上增強或改善植物的耐熱性及耐乾燥性者，其特徵為，耐受性增強劑的產生器具備：頂部密封、底部開放的圓筒狀可撓性容器，***於前述可撓性容器內部的收納空間之含有選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物的第1易碎性容器，附加在前述可撓性容器的底部之附件，及安裝在前述附件與前述可撓性容器之間的滲透膜，其中，前述附件具有與前述可撓性容器的內面相對向之側面及與前述可撓性容器的底部相對向之上表面，前述附件在前述表面具有貫穿孔，同時在前述側面具有一個或多個空隙，且在第1易碎性容器的外側且可撓性容器內收納含有檸檬酸三乙酯的第2易碎性容器。 [17] A tolerance enhancer generator (hereinafter also referred to as "the tolerance enhancer generator (4) of the present invention"), wherein the tolerance enhancer is used to enhance or improve the heat resistance and desiccation resistance of plants for more than 2 months, and wherein the tolerance enhancer generator comprises: a cylindrical flexible container with a sealed top and an open bottom, and a storage space inserted into the flexible container containing a substance selected from the group consisting of α , β -enal, α , β -enone, γ , δ -enal, γ , δ -enone, δ , ε -enal, δ , ε -enone, ε , ζ -enal and ε , ζ-enone . A first frangible container containing at least one unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal of the first frangible container, an attachment attached to the bottom of the above-mentioned flexible container, and a permeable membrane installed between the above-mentioned attachment and the above-mentioned flexible container, wherein the above-mentioned attachment has a side surface opposite to the inner surface of the above-mentioned flexible container and an upper surface opposite to the bottom of the above-mentioned flexible container, the above-mentioned attachment has a through hole on the above-mentioned surface and one or more gaps on the above-mentioned side surface, and a second frangible container containing triethyl citrate is accommodated on the outer side of the first frangible container and in the flexible container.

〔18〕一種耐受性增強劑的產生器(以下亦稱「本發明的耐受性增強劑的產生器(4’)」，該耐受性增強劑係用以持續2個月以上增強或改善植物的耐熱性及耐乾燥性者，其中，耐受性增強劑的產生器具備：頂部密封、底部開放的圓筒狀可撓性容器，***於前述可撓性容器內部的收納空間之含有選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物和檸檬酸三乙酯之組成物的至少一個易碎性容器，附加在前述可撓性容器的底部之附件和，及安裝在前述附件與前述可撓性容器之間的滲透膜，其中，前述附件具有與前述可撓性容器的內面相對向之側面及與前述可撓性容器的底部相對向之上表面，前述附件在前述表面具有貫穿孔，同時在前述側面具有一個或多個空隙。 [18] A tolerance enhancer generator (hereinafter also referred to as "the tolerance enhancer generator (4') of the present invention"), wherein the tolerance enhancer is used to enhance or improve the heat resistance and desiccation resistance of plants for more than 2 months, wherein the tolerance enhancer generator comprises: a cylindrical flexible container with a sealed top and an open bottom, and a storage space inserted into the flexible container containing a substance selected from the group consisting of α , β -enal, α , β -enone, γ , δ -enal, γ , δ -enone, δ , ε -enal, δ , ε -enone, ε , ζ -enal and ε , ζ At least one frangible container containing at least one unsaturated carbonyl compound with 4 to 9 carbon atoms and no unsaturated bond at the terminal of at least one of the group consisting of cyclopentane and cyclopentane, and triethyl citrate, an attachment attached to the bottom of the above-mentioned flexible container, and a permeable membrane installed between the above-mentioned attachment and the above-mentioned flexible container, wherein the above-mentioned attachment has a side surface opposite to the inner surface of the above-mentioned flexible container and an upper surface opposite to the bottom of the above-mentioned flexible container, and the above-mentioned attachment has a through hole on the above-mentioned surface, and has one or more gaps on the above-mentioned side surface.

〔19〕如上述〔16〕至〔18〕中任一項所述之產生器(以下，亦稱「本發明的耐受性增強劑的產生器(5)」)，其中，前述可撓性容器和前述滲透膜之間更具有至少具有一個開口部的支撐具。 [19] A generator as described in any one of [16] to [18] above (hereinafter also referred to as "the generator of the tolerance enhancer of the present invention (5)"), wherein a support having at least one opening is provided between the flexible container and the permeable membrane.

〔20〕如上述〔13〕至〔19〕中任一項所述之產生器，其中，可撓性容器係選自由聚丙烯、聚乙烯、聚胺酯、聚醯胺及聚氯乙烯所組成之群組中的樹脂製，且易碎性容器為玻璃安瓿。 [20] A generator as described in any one of [13] to [19] above, wherein the flexible container is made of a resin selected from the group consisting of polypropylene, polyethylene, polyurethane, polyamide and polyvinyl chloride, and the fragile container is a glass ampoule.

目前，尚未有可整個季節使用的耐受性增強劑之報告例。依照本發明，可提供一種植物之耐受性增強劑，係藉由使用含有選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和 羰基化合物及檸檬酸三乙酯植物的組成物，可持續2個月以上增強或改善植物的耐熱性、耐乾燥性等耐受性，藉此，可在農業現場中不需頻繁更換，可整個季節(夏季的2個月期間)使用。此外，依照本發明，可提供簡便且有效的產生該耐受性增強劑之器具。 At present, there is no report of a tolerance enhancer that can be used throughout the season. According to the present invention, a plant tolerance enhancer can be provided, which can enhance or improve the heat resistance, drought resistance and other tolerance of plants for more than 2 months by using a composition containing at least one unsaturated carbonyl compound with 4 to 9 carbon atoms and no unsaturated bond at the end selected from the group consisting of α , β -enal, α , β -enone, γ, δ -enal, γ , δ -enone, δ, ε-enal, δ, ε-enone, ε, ζ-enal and ε, ζ-enone, and triethyl citrate. It can be used throughout the season (two months in summer) without frequent replacement in agricultural fields. In addition, according to the present invention, a simple and effective device for producing the tolerance enhancer can be provided.

1:可撓性容器 1: Flexible container

2,9,18:易碎性容器(玻璃安瓿) 2,9,18: Fragile containers (glass ampoules)

3,10,19:內部液(有效成分化合物等) 3,10,19: Internal fluid (active ingredient compounds, etc.)

4:吸收性材料 4: Absorbent materials

5:散發孔 5: Diffusion holes

6,16:掛鈎 6,16:Hook

7,17:收納空間 7,17: Storage space

8,20:外部液(含有檸檬酸三乙酯之液劑) 8,20: External liquid (liquid containing triethyl citrate)

11:空隙 11: Gap

12,23:滲透膜 12,23: Osmotic membrane

13,21:附件 13,21: Attachment

14,25:貫穿孔 14,25: Perforation

15:底部 15: Bottom

22:支撐具 22: Support device

24:接合處 24:Joint

26:開口部 26: Opening

圖1係呈示本發明的耐受性增強劑的產生器(1)之一型態的剖面圖。 FIG1 is a cross-sectional view showing one type of the tolerance enhancer generator (1) of the present invention.

圖2係呈示本發明的耐受性增強劑的產生器(3)之一型態的剖面圖及分解圖。 FIG2 is a cross-sectional view and an exploded view showing one type of the tolerance enhancer generator (3) of the present invention.

圖3係呈示本發明的耐受性增強劑的產生器(5)之一型態的剖面圖及部分放大圖。 FIG3 is a cross-sectional view and a partially enlarged view showing one type of the tolerance enhancer generator (5) of the present invention.

以下詳細說明本發明。 The present invention is described in detail below.

(本發明的耐受性增強劑) (Tolerance enhancer of the present invention)

本發明的耐受性增強劑中之有效成分係選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵(即，碳-碳雙鍵)的碳數4至9之不飽和羰基化合物(以下亦稱為「本發明的有效成分化合物」)。本發明的有效成分化合物係以選自由α,β-烯醛、γ,δ-烯醛、δ,ε-烯醛及ε,ζ-烯醛所組成之群組中的至少1種為佳，以α,β-烯醛更佳(特別佳的係2-己烯醛)。 The active ingredient in the tolerance enhancer of the present invention is an unsaturated carbonyl compound with 4 to 9 carbon atoms and no unsaturated bond (i.e., carbon-carbon double bond) at the end of at least one of the group consisting of α, β-enal, α, β-enone, γ , δ - enal , γ , δ - enone , δ, ε-enal, δ, ε-enone, ε , ζ -enal and ε , ζ -enone (hereinafter also referred to as "the active ingredient compound of the present invention"). The active ingredient compound of the present invention is preferably at least one of the group consisting of α , β -enal, γ , δ -enal, δ , ε -enal and ε , ζ -enal, and α , β -enal is more preferred (particularly preferred is 2-hexenal).

本發明的有效成分化合物雖然可以係鏈狀(直鏈狀或分枝鏈狀)及環狀的任一種，但較佳的係鏈狀，更佳的係直鏈狀。 Although the active ingredient compound of the present invention can be in the form of a chain (straight chain or branched chain) or a ring, it is preferably in the form of a chain, and more preferably in the form of a straight chain.

本發明的有效成分化合物具有異構物(E體、Z體等)時，可使用任一種異構物，此外，也可使用以任意比率含有多種異構物的混合物。 When the active ingredient compound of the present invention has isomers (E-isomer, Z-isomer, etc.), any one isomer may be used, and a mixture containing a plurality of isomers in any ratio may also be used.

本發明的有效成分化合物之具體例可列舉例如：2-丁烯醛、2-戊烯醛、2-己烯醛、2-庚烯醛、2-辛烯醛、2-壬烯醛等α,β-烯醛；3-戊烯-2-酮、2-己烯-3-酮、3-庚烯-2-酮、2-辛烯-4-酮等α,β-烯酮；4-己烯醛、4-庚烯醛等γ,δ-烯醛；5-辛烯-2-酮等γ,δ-烯酮；5-庚烯醛、5-辛烯醛、5-壬烯醛等δ,ε-烯醛；6-壬烯-2-酮等δ,ε-烯酮；6-辛烯醛、6-壬烯醛等ε,ζ-烯醛；及7-壬烯-2-酮等ε,ζ-烯酮等。其中係以2-丁烯醛、2-戊烯醛、2-己烯醛、2-庚烯醛、2-辛烯醛、2-壬烯醛等α,β-烯醛；4-己烯醛、4-庚烯醛等γ,δ-烯醛；5-庚烯醛、5-辛烯醛、5-壬烯醛等δ,ε-烯醛；6-辛烯醛、6-壬烯醛等ε,ζ-烯醛為佳，以2-丁烯醛、2-戊烯醛、2-己烯醛、2-庚烯醛、2-辛烯醛、2-壬烯醛等α,β-烯醛更佳，以2-己烯醛特別佳。 Specific examples of the active ingredient compound of the present invention include α, β-enaldehydes such as 2-butenal, 2-pentenal, 2-hexenal, 2-heptenal, 2-octenal, and 2-nonenal; α , β -enones such as 3-penten-2-one, 2-hexen-3-one, 3-hepten-2-one, and 2-octen- 4 -one; γ , δ -enaldehydes such as 4-hexenal and 4-heptenal; γ , δ -enaldehydes such as 5-octen-2-one; δ , ε -enaldehydes such as 5-heptenal, 5-octenal, and 5-nonenal; δ , ε -enaldehydes such as 6-nonen-2-one; ε , ζ -enaldehydes such as 6-octenal and 6-nonenal; and ε , ζ -enones such as 7-nonen-2-one. Among them, α , β -enals such as 2-butenal, 2-pentenal, 2-hexenal, 2-heptenal, 2-octenal, and 2-nonenal; γ , δ -enals such as 4-hexenal and 4-hexenal; δ , ε -enals such as 5-hexenal, 5-octenal, and 5-nonenal; ε , ζ -enals such as 6-octenal and 6-nonenal are preferred, α , β -enals such as 2-butenal, 2-pentenal, 2-hexenal, 2-hexenal, 2-heptenal, 2- octenal , and 2 -nonenal are more preferred, and 2-hexenal is particularly preferred.

本發明的有效成分化合可單獨使用，也可將2種以上組合使用。 The active ingredient compounds of the present invention can be used alone or in combination of two or more.

本發明的有效成分化合之含量並無特別的限制，但相對於耐受性增強劑整體(100質量%)，通常係大約0.001至大約90質量%，以大約0.01至大約50質量%為佳。 The content of the active ingredient compound of the present invention is not particularly limited, but is generally about 0.001 to about 90 mass %, preferably about 0.01 to about 50 mass %, relative to the entire tolerance enhancer (100 mass %).

本發明的有效成分化合物係非常不穩定之物質，在僅有本發明的有效成分化合物時，因容易在空氣中氧化而轉變成另一種氣味成分，故難以長期持續植物的耐受性增強效果。本發明的耐受性增強劑中，檸檬酸三乙酯係用以保持本發明的有效成分化合物之穩定性、賦與緩釋性、可長期持續作用效果用之必 需成分。檸檬酸三乙酯可直接單獨使用市售品，也可視需要而和其他的添加劑混合使用。 The active ingredient compound of the present invention is a very unstable substance. When there is only the active ingredient compound of the present invention, it is easy to oxidize in the air and transform into another odor component, so it is difficult to maintain the tolerance enhancement effect of plants for a long time. In the tolerance enhancer of the present invention, triethyl citrate is a necessary ingredient for maintaining the stability of the active ingredient compound of the present invention, imparting sustained release, and maintaining the effect for a long time. Triethyl citrate can be used directly alone as a commercial product, or it can be mixed with other additives as needed.

本說明書中的「含有檸檬酸三乙酯之液劑」可以係由檸檬酸三乙酯所構成之液劑，也可以係含有檸檬酸三乙酯及其他的添加劑之液劑。不論該「液劑」的黏度如何，只要係液體即可，此外，可以係檸檬酸三乙酯溶解在液體的非活性載體中之溶液，也可以係液體的非活性載體和檸檬酸三乙酯之懸浮液。作為含有檸檬酸三乙酯之液劑，係以由檸檬酸三乙酯所構成之液劑為佳。 The "liquid containing triethyl citrate" in this manual can be a liquid composed of triethyl citrate, or a liquid containing triethyl citrate and other additives. Regardless of the viscosity of the "liquid", as long as it is a liquid, it can be a solution of triethyl citrate dissolved in a liquid inactive carrier, or a suspension of a liquid inactive carrier and triethyl citrate. As a liquid containing triethyl citrate, a liquid composed of triethyl citrate is preferred.

本發明的耐受性增強劑中檸檬酸三乙酯之含量並無特別的限制，但相對於耐受性增強劑整體(100質量%)，通常係大約10至99.999質量%，以大約50至99.99質量%為佳。檸檬酸三乙酯之含量為上述的範圍內時，本發明的有效成分化合物之穩定效果優良(抗氧化效果)，且可經2個月以上的長期間增強或改善植物的耐受性(耐熱性、耐乾燥性及其他的耐受性)。 The content of triethyl citrate in the tolerance enhancer of the present invention is not particularly limited, but relative to the entire tolerance enhancer (100 mass%), it is usually about 10 to 99.999 mass%, preferably about 50 to 99.99 mass%. When the content of triethyl citrate is within the above range, the active ingredient compound of the present invention has an excellent stabilizing effect (antioxidant effect), and can enhance or improve the tolerance of plants (heat resistance, drought resistance and other tolerance) for a long period of more than 2 months.

本發明的耐受性增強劑之增強或改善植物的耐受性(耐熱性、耐乾燥及其他的耐受性)之效果，可從開始使用起至少持續2個月，較佳的係持續3個月以上。 The effect of the tolerance enhancer of the present invention in enhancing or improving the tolerance (heat resistance, drought resistance and other tolerance) of plants can last for at least 2 months from the start of use, and preferably for more than 3 months.

本說明書中，「耐熱性」係指對於暫時或永久曝晒的高溫(高溫狀態)的耐受性(耐高溫性)，高溫係指例如25℃以上(例如27至70℃)，以30℃以上(例如32至60℃)的溫度為佳，以35℃以上(例如38至55℃)的溫度更佳，以40℃以上(例如42至50℃)的溫度特別佳。 In this manual, "heat resistance" refers to the tolerance (high temperature resistance) to high temperature (high temperature state) temporarily or permanently exposed to the sun. High temperature refers to, for example, a temperature of 25°C or above (e.g., 27 to 70°C), preferably a temperature of 30°C or above (e.g., 32 to 60°C), more preferably a temperature of 35°C or above (e.g., 38 to 55°C), and particularly preferably a temperature of 40°C or above (e.g., 42 to 50°C).

本說明書中，「耐乾燥性」係指缺乏水分的狀態(乾燥地或乾旱)、或即使係限制用水的土地也不枯萎，並可保持生長狀態之植物的耐受性。 In this manual, "drought tolerance" refers to the tolerance of plants to maintain growth in a state of lack of water (dry land or drought) or in a land with limited water use without withering.

本說明書中，「其他的耐受性」係指耐熱性、耐乾燥性以外的環境壓力耐受性，可列舉例如：選自耐傷害性、耐光性(耐紫外線性)、耐氧化性、耐低溫性(低溫耐受性)、耐滲透壓性及耐鹼性中的至少1種之環境壓力耐受性。 In this manual, "other resistance" refers to environmental stress resistance other than heat resistance and dryness resistance, and examples thereof include: at least one environmental stress resistance selected from damage resistance, light resistance (UV resistance), oxidation resistance, low temperature resistance (low temperature tolerance), osmotic pressure resistance, and alkali resistance.

本發明的耐受性增強劑可以係僅由前述末端不具有不飽和鍵的碳數4至9的不飽和羰基化合物(本發明的有效成分化合物)和檸檬酸三乙酯所構成，或可視需要而以適當比例調配其他的添加劑而製劑化。具體上，可將本發明的有效成分化合物和檸檬酸三乙酯視需要而以適當比例調配其他的添加劑，使其溶解、懸浮、混合、含浸、吸吸、附著等，製劑化成適當的劑型，例如懸浮劑、乳懸浮劑、乳劑、液劑、水合劑、顆粒水合劑、粒劑、粉劑、錠劑、巨型劑等使用，此等之中，係以液劑為佳，液劑可直接使用，或使其含浸在固體的非活性載體中使用。 The tolerance enhancer of the present invention may be composed only of the unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal (the active ingredient compound of the present invention) and triethyl citrate, or may be formulated by mixing other additives in appropriate proportions as required. Specifically, the active ingredient compound of the present invention and triethyl citrate can be mixed with other additives in appropriate proportions as needed to dissolve, suspend, mix, impregnate, absorb, attach, etc., and be formulated into appropriate dosage forms, such as suspensions, emulsions, emulsions, liquids, hydrates, granular hydrates, granules, powders, tablets, giant doses, etc. Among these, liquids are preferred. Liquids can be used directly or impregnated in solid inactive carriers for use.

可調配在本發明的耐受性增強劑中之「其他的添加劑」，可例示非活性載體、補助劑等。 The "other additives" that can be formulated in the tolerance enhancer of the present invention include, for example, inactive carriers, supplements, etc.

非活性載體可以係固體及液體的任一種。固體的非活性載體可列舉：例如植物質粉末類(例如大豆粉、穀物粉、木粉、樹皮粉、鋸木粉、煙梗粉、核桃殼粉、麩皮、纖維素粉、球狀纖維素等)、粉碎合成樹脂等合成聚合物、黏土類(例如高嶺土、膨潤土、酸性黏土等)、滑石類(例如滑石、葉蠟石(pyrophyllite)等)、二氧化矽類(例如矽藻土、矽砂、雲母、矽酸鈣等)、活性炭、天然礦物質類(例如硫磺粉、浮石、鎂鋁海泡石(attapulgite)、沸石等)、煅燒矽藻土、碎磚、粉煤灰、沙子、塑膠載體(例如聚乙烯、聚丙烯、聚偏二氯乙烯等)、無機礦物性粉末(例如碳酸鈣、磷酸鈣等)、化學肥料(例如硫酸銨、磷酸銨、硝酸銨、尿素、氯化銨等)、堆肥等。此等載體可單獨使用，或以2種以上的混合物形態使用。可 含浸本發明的組成物而使用之非活性載體，係以將矽酸鈣粉末錠劑化者(Floralette(註冊商標))，LETS ONE股份有限公司製造)、生物分解性球狀纖維素(Visco Pearl(註冊商標)；Rengo股份有限公司製造)等為佳。 The inactive carrier may be any of a solid and a liquid. Examples of solid inactive carriers include plant powders (e.g., soybean powder, grain powder, wood powder, bark powder, sawdust powder, tobacco stem powder, walnut shell powder, bran, cellulose powder, spherical cellulose, etc.), synthetic polymers such as pulverized synthetic resins, clays (e.g., kaolin, bentonite, acid clay, etc.), talcs (e.g., talc, pyrophyllite, etc.), silicas (e.g., diatomaceous earth, silica sand, mica, calcium silicate, etc.), activated carbon, natural minerals (such as sulfur powder, pumice, magnesium aluminum sepiolite (attapulgite), zeolite, etc.), calcined diatomaceous earth, crushed bricks, fly ash, sand, plastic carriers (such as polyethylene, polypropylene, polyvinylidene chloride, etc.), inorganic mineral powders (such as calcium carbonate, calcium phosphate, etc.), chemical fertilizers (such as ammonium sulfate, ammonium phosphate, ammonium nitrate, urea, ammonium chloride, etc.), compost, etc. These carriers can be used alone or in the form of a mixture of two or more. The inactive carrier that can be used to impregnate the composition of the present invention is preferably a tablet of calcium silicate powder (Floralette (registered trademark)), manufactured by LETS ONE Co., Ltd.), biodegradable spherical cellulose (Visco Pearl (registered trademark); manufactured by Rengo Co., Ltd.), etc.

液體的非活性載體可列舉例如：水、醇類(例如甲醇、乙醇、異丙醇、丁醇、乙二醇等)、酮類(例如丙酮、甲基乙基酮、甲基異丁酮、二異丁基酮、環己酮等)、醚類(例如***、二噁烷、賽珞蘇(cellosolve)、二丙醚、四氫呋喃等)、脂肪族烴類(例如煤油、礦物油等)、芳香族烴類(例如苯、甲苯、二甲苯、溶劑石腦油、烷基萘等)、鹵化烴類(例如二氯乙烷、氯仿、四氯化碳等)、酯類(例如乙酸乙酯、鄰苯二甲酸二異丙酯、鄰苯二甲酸二丁酯、鄰苯二甲酸二辛酯等)、腈類(例如乙腈等)、二甲基亞碸類等，此等載體可單獨使用，或以2種以上的混合物形態使用。 Examples of liquid inactive carriers include water, alcohols (e.g., methanol, ethanol, isopropanol, butanol, ethylene glycol, etc.), ketones (e.g., acetone, methyl ethyl ketone, methyl isobutyl ketone, diisobutyl ketone, cyclohexanone, etc.), ethers (e.g., diethyl ether, dioxane, cellosolve, dipropyl ether, tetrahydrofuran, etc.), aliphatic hydrocarbons (e.g., kerosene, mineral oil, etc.), aromatic hydrocarbons (e.g., Hydrocarbons (such as benzene, toluene, xylene, solvent naphtha, alkylnaphthalene, etc.), halogenated hydrocarbons (such as ethylene dichloride, chloroform, carbon tetrachloride, etc.), esters (such as ethyl acetate, diisopropyl phthalate, dibutyl phthalate, dioctyl phthalate, etc.), nitriles (such as acetonitrile, etc.), dimethyl sulfoxides, etc. These carriers can be used alone or in the form of a mixture of two or more.

補助劑可視需要而添加，可單獨使用，或可將2種以上組合而使用。可將本發明的有效成分化合物之乳化、分散、可溶化等用之界面活性劑作為補助劑使用。界面活性劑雖然可列舉例如：聚氧乙烯烷醚、聚氧乙烯烷基芳基醚、聚氧乙烯高級脂肪酸酯、聚氧乙烯樹脂酸酯、聚氧乙烯脫水山梨醇烷化物、聚氧乙烯脫水山梨醇單月桂酸酯、聚氧乙烯脫水山梨醇單油酸酯、烷基芳基磺酸鹽、萘磺酸縮合物、木質素磺酸鹽、高級醇硫酸酯等，但並不限於此等者。 The additive can be added as needed, and can be used alone or in combination of two or more. The surfactant used for emulsification, dispersion, solubilization, etc. of the active ingredient compound of the present invention can be used as an additive. Although the surfactant can be listed as follows: polyoxyethylene alkyl ether, polyoxyethylene alkyl aryl ether, polyoxyethylene higher fatty acid ester, polyoxyethylene ester, polyoxyethylene sorbitan alkylate, polyoxyethylene sorbitan monolaurate, polyoxyethylene sorbitan monooleate, alkyl aryl sulfonate, naphthalenesulfonic acid condensate, lignin sulfonate, higher alcohol sulfate, etc., but it is not limited to these.

為了使本發明的有效成分化合物分散穩定化、黏著、結合等，例如可使用澱粉、甲基纖維素、羧甲基纖維素、***膠、聚乙烯醇、松根油、糠油、膨潤土、木質素磺酸鹽等作為補助劑。為了改良流動性，可使用例如聚乙二醇(PEG)、液體石蠟、蠟、硬脂酸鹽、磷酸烷酯等作為補助劑。作為消泡劑，可使用例如矽酮油等作為補助劑。 In order to disperse, stabilize, adhere, and bind the active ingredient compound of the present invention, for example, starch, methyl cellulose, carboxymethyl cellulose, gum arabic, polyvinyl alcohol, pine root oil, bran oil, bentonite, lignin sulfonate, etc. can be used as additives. In order to improve fluidity, for example, polyethylene glycol (PEG), liquid paraffin, wax, stearate, alkyl phosphate, etc. can be used as additives. As a defoaming agent, for example, silicone oil can be used as an additive.

作為本發明的有效成分化合物之揮發性保持劑(例如，保持劑、芳香劑等)，可視需要而適量調配：麝香、鯰魚香、龍涎香等動物性保持劑；雪松油(cedarwood oil)、香根草油(vetiva oil)、油樹脂(oleoresin)、香脂(balsam)、樹脂狀物質(resinoid)等植物性保持劑；香草醛(vanillin)等人造保持劑。此等物質的分子量大並缺乏揮發性，故適合作為保持劑。此外，作為可調配在本發明的耐受性增強劑中之其他較佳的揮發性保持劑或非活性載體，可列舉選自由三環癸烷、三環十二烷(商品名「Aisawa-D」，出光石油化學股份有限公司製造)、金剛烷、2,2-二甲基-1,3-丙二醇、2,4,6-三第三丁基-1,3,5-三噁烷及2,4,6-三異丙基-1,3,5-三噁烷(商品名「Sansaburi(註冊商標)B」，小川香料股份有限公司製造)所組成之群組中的1種或2種以上之昇華性物質。調配此昇華性物質即可調節本發明的有效成分化合物之揮散性(揮散量、揮散速度等)。該昇華性物質係以2,4,6-三異丙基-1,3,5-三噁烷特別佳。 As the volatile retaining agent (e.g., retaining agent, aromatic agent, etc.) of the active ingredient compound of the present invention, the following can be appropriately prepared as needed: animal retaining agents such as musk, catfish scent, and ambergris; plant retaining agents such as cedarwood oil, vetiva oil, oleoresin, balsam, and resinoid; and artificial retaining agents such as vanillin. These substances have large molecular weights and lack volatility, so they are suitable as retaining agents. In addition, as other preferred volatility retainers or inactive carriers that can be formulated in the tolerance enhancer of the present invention, one or more sublimable substances selected from the group consisting of tricyclodecane, tricyclododecane (trade name "Aisawa-D", manufactured by Idemitsu Petrochemical Co., Ltd.), adamantane, 2,2-dimethyl-1,3-propanediol, 2,4,6-tri-tert-butyl-1,3,5-trioxane and 2,4,6-triisopropyl-1,3,5-trioxane (trade name "Sansaburi (registered trademark) B", manufactured by Ogawa Flavor Co., Ltd.) can be listed. The volatility (volatility amount, volatility rate, etc.) of the active ingredient compound of the present invention can be adjusted by formulating such sublimable substances. The sublimable substance is particularly preferably 2,4,6-triisopropyl-1,3,5-trioxane.

在不影響本發明的有效成分化合物之耐受性增強效果的範圍中，本發明的耐受性增強劑可視需要而適量調配其他的有效成分(以下亦稱「併用劑或組合劑」)。 As long as the tolerance enhancing effect of the active ingredient compound of the present invention is not affected, the tolerance enhancer of the present invention can be formulated with other active ingredients (hereinafter also referred to as "concomitant agents or combination agents") in appropriate amounts as needed.

併用劑並無特別的限制，可列舉例如：耐熱性、耐乾燥性以外的環境壓力耐受性劑、除蟲劑、昆蟲(蜜蜂等)引誘劑、抗病蟲害性誘發劑、費洛蒙干擾劑等。 There are no special restrictions on the concomitant agents, for example: environmental stress tolerance agents other than heat resistance and dryness resistance, insecticides, insect (bee, etc.) attractants, pest and disease resistance attractants, pheromone interferers, etc.

耐熱性、耐乾燥性以外的環境壓力耐受性劑可列舉例如：(Z)-3-乙酸己烯酯等。 Environmental stress resistance agents other than heat resistance and dryness resistance include, for example: (Z)-3-hexenyl acetate, etc.

除蟲劑可列舉例如：檸檬烯、蒎烯、芳樟醇、萜品烯、除蟲菊精(pyrethroid)化合物等。 Examples of insecticides include: limonene, pinene, linalool, terpinene, pyrethroid compounds, etc.

昆蟲(蜜蜂等)引誘劑可列舉例如：檸檬醛、檜烯、α-蒎烯、乙酸己烯酯、己酸己酯、(E)-2-己烯基己酸酯、丁酸辛酯、乙酸己酯等。 Insect (bee, etc.) attractants include, for example, citral, pinene, α -pinene, hexenyl acetate, hexyl caproate, (E)-2-hexenyl caproate, octyl butyrate, hexyl acetate, etc.

抗病蟲害性誘發劑可列舉例如：茉莉酸甲酯、水楊酸甲酯、(Z)-3-乙酸己烯酯、β-羅勒烯等。 Examples of pest and disease resistance inducers include methyl jasmonate, methyl salicylate, (Z)-3-hexenyl acetate, β -ocimene, etc.

費洛蒙干擾劑可列舉例如：乙酸(Z)-11-十四烯酯、乙酸(Z)-9-十四烯酯、丁酸辛酯等。 Examples of pheromone interferers include (Z)-11-tetradecene acetate, (Z)-9-tetradecene acetate, octyl butyrate, etc.

可適用本發明的耐受性增強劑之植物，只要係具有氣孔的植物，即無特別的限制。較佳的係農園藝用植物。具體上的植物(特別係農園藝用植物)可列舉例如：穀類(稻、大麥、小麥、黑麥、燕麥、玉米等)、豆類(大豆、小豆、蠶豆、豌豆、花生等)、果樹‧果實類(蘋果、柑橘類、梨、葡萄、桃、梅、櫻桃、核桃、杏仁、香蕉、草莓等)、蔬菜類(高麗菜、蕃茄、茄、波菜、綠花椰菜、萵苣、洋蔥、蔥、甜椒等)、根莖類(胡蘿蔔、馬鈴薯、甘薯、蘿蔔、蕪菁等)、加工用作物類(棉、麻、桑葚、結香(三叉)、菜籽、甜菜、啤酒花、甘蔗、甜菜、橄欖、橡膠、咖啡、菸草、茶等)、瓜類(南瓜、黃瓜、西瓜、洋香瓜等)、牧草類(果園草、高粱、堤摩西、三葉草、苜蓿等)、芝類(高麗草、翦股穎等)、花卉類(菊、玫瑰、蘭等)、香料等用作物類(薰衣草、迷迭香、百里香、歐芹、胡椒、生薑等)等。 Plants to which the tolerance enhancer of the present invention can be applied are not particularly limited as long as they are plants with stomata. Preferred are agricultural and horticultural plants. Specific plants (especially agricultural and horticultural plants) include, for example, cereals (rice, barley, wheat, rye, oats, corn, etc.), beans (soybeans, adzuki beans, broad beans, peas, peanuts, etc.), fruit trees and fruits (apples, citrus fruits, pears, grapes, peaches, plums, cherries, walnuts, almonds, bananas, strawberries, etc.), vegetables (cabbage, tomatoes, eggplants, spinach, broccoli, lettuce, onions, scallions, bell peppers, etc.), roots (carrots, potatoes, sweet potatoes, radishes, turnips, etc.), and vegetables (such as celery, cauliflower, celery, onions, and sweet peppers). , etc.), processed crops (cotton, linen, mulberry, knotweed, rapeseed, beet, hops, sugar cane, beet, olive, rubber, coffee, tobacco, tea, etc.), melons (pumpkin, cucumber, watermelon, cantaloupe, etc.), forage (orchard grass, sorghum, Timothy, clover, alfalfa, etc.), grass (Korean grass, bentgrass, etc.), flowers (chrysanthemum, rose, orchid, etc.), spices (lavender, rosemary, thyme, parsley, pepper, ginger, etc.), etc.

本說明書中，「適用於植物」或「處理植物」係指使本發明的耐受性增強劑到達目標植物中。因此，例如，可直接施用本發明的耐受性增強劑，或用水等適當稀釋後，以誘發目標植物的耐受性之有效量施用在目標植物或目標植物的載體上即可。例如可適當使用朝目標植物直接散布、植物基部處理、作物處理、土壤混合等施用方法。此外，水耕栽培中的水耕液也可用本發明的耐受 性增強。本發明的耐受性增強方法係將本發明的耐受性增強劑施用在栽培載體上的方法，即可適用植物基部處理、作物處理、土壤混合、水耕液處理等施用方法。另外，在塑膠(聚乙烯)溫室等密閉空間中，也可藉由將本發明的耐受性增強劑之有效成分化合物揮散(氣化)而處理(使其到達植物中)植物。此外，用本發明的耐受性增強劑處理植物的時期並無特別的限制，例如係以預期會發生高溫損壞的時期之前為佳。 In this specification, "apply to plants" or "treat plants" means that the tolerance enhancer of the present invention reaches the target plant. Therefore, for example, the tolerance enhancer of the present invention can be directly applied, or after being appropriately diluted with water or the like, applied to the target plant or the carrier of the target plant in an effective amount to induce tolerance of the target plant. For example, application methods such as direct spreading toward the target plant, plant base treatment, crop treatment, soil mixing, etc. can be appropriately used. In addition, the hydroponic solution in hydroponic cultivation can also be enhanced with the tolerance of the present invention. The tolerance enhancement method of the present invention is a method of applying the tolerance enhancer of the present invention to a cultivation carrier, that is, application methods such as plant base treatment, crop treatment, soil mixing, hydroponic solution treatment, etc. can be applied. In addition, in a closed space such as a plastic (polyethylene) greenhouse, the active ingredient compound of the tolerance enhancer of the present invention can also be dispersed (gasified) to treat (allow it to reach the plant) the plant. In addition, there is no particular restriction on the period of treating the plant with the tolerance enhancer of the present invention, for example, it is preferably before the period when high temperature damage is expected to occur.

本說明書中，栽培載體係指栽培植物用之支撐體，只要係培育植物生長的材質即可。例如包括所謂各類土壤、育苗墊、水等。具體上可列舉例如：沙、蛭石、棉、紙、矽藻土、瓊脂(agar，寒天)、凝膠狀材質、高分子材料、岩棉、玻璃棉、木片、樹皮、浮石等。 In this manual, the cultivation carrier refers to the support used to cultivate plants, as long as it is a material for cultivating plant growth. For example, it includes various types of soil, seedling mats, water, etc. Specifically, it can be listed as: sand, vermiculite, cotton, paper, diatomaceous earth, agar, gel-like materials, polymer materials, rock wool, glass wool, wood chips, bark, pumice, etc.

本發明的耐受性增強劑之使用量(施用量、處理量)係依本發明的有效成分化合物之調配比例、氣候條件、製劑形態、施用時期、施用方法、施用場所、目標植物等而異，但通常作為本發明的有效成分化合物，係以每天大約0.001g至大約10g的範圍中選擇適當的量施用即可，以大約0.005g至大約1g的範圍為佳。此外，相對於前述碳數4至9的不飽和羰基化合物1g，檸檬酸三乙酯的使用量(施用量、處理量)通常係大約0.5g以上10g以下，以大約1g以上8g以下為佳，以大約2g以上5g以下更佳。 The dosage (application amount, treatment amount) of the tolerance enhancer of the present invention varies depending on the formulation ratio of the active ingredient compound of the present invention, climatic conditions, formulation form, application period, application method, application site, target plant, etc., but usually, as the active ingredient compound of the present invention, an appropriate amount is selected from the range of about 0.001g to about 10g per day, preferably about 0.005g to about 1g. In addition, relative to 1g of the aforementioned unsaturated carbonyl compound with 4 to 9 carbon atoms, the dosage (application amount, treatment amount) of triethyl citrate is usually about 0.5g to 10g, preferably about 1g to 8g, and more preferably about 2g to 5g.

(本發明的耐受性增強方法) (Tolerance enhancement method of the present invention)

本發明的耐受性增強方法只要係可將本發明的耐受性增強劑適用於植物上的方法，即無特別的限制，尤其，就使本發明的有效成分化合物在塑膠型溫室等密閉空間中揮發而處理植物，以在2個月以上持續增強或改善植物的耐熱性及 耐乾燥性用的方法而予，係適用包括下述步驟的植物耐受性增強方法(本發明的耐受性增強方法)： The tolerance enhancing method of the present invention is not particularly limited as long as it is a method that can apply the tolerance enhancing agent of the present invention to plants. In particular, the method of treating plants by volatilizing the active ingredient compound of the present invention in a closed space such as a plastic greenhouse to continuously enhance or improve the heat resistance and drought resistance of plants for more than 2 months is applicable to a method for enhancing plant tolerance (the tolerance enhancing method of the present invention) comprising the following steps:

(1)將選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物和檸檬酸三乙酯混合，而調製含有該等之組成物(本發明的組成物)的步驟；及 (1) a step of preparing a composition containing at least one unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal selected from the group consisting of α, β - enal , α , β - enone , γ , δ -enal, γ , δ-enone, δ, ε -enal, δ, ε-enone, ε, ζ-enal and ε , ζ -enone by mixing triethyl citrate; and

(2)將上述末端不具有不飽和鍵的碳數4至9的不飽和羰基化合物(即，本發明的有效成分化合物)釋放在大氣中之步驟。 (2) A step of releasing the above-mentioned unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal (i.e., the active ingredient compound of the present invention) into the atmosphere.

本發明的耐受性增強方法中使用的本發明之耐受性增強劑的有效成分化合物，可使用前述的末端不具有不飽和鍵(即，碳-碳雙鍵)的碳數4至9之不飽和羰基化合物(本發明的有效成分化合物)，較佳的係選自由α,β-烯醛、γ,δ-烯醛、δ,ε-烯醛、ε,ζ-烯醛所組成之群組中的至少1種，更佳的係α,β-烯醛(特別佳的係2-己烯醛)。 The active ingredient compound of the tolerance enhancer of the present invention used in the tolerance enhancing method of the present invention can be the unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond (i.e., carbon-carbon double bond) at the terminal as mentioned above (active ingredient compound of the present invention), preferably at least one selected from the group consisting of α , β -enal, γ , δ -enal, δ , ε -enal, ε , ζ -enal, more preferably α , β -enal (particularly preferably 2-hexenal).

本發明的耐受性增強方法，只要係包含將本發明的有效成分化合物和檸檬酸三乙酯混合，調製含有該等的組成物(本發明的組成物)之步驟，及將本發明的有效成分化合物釋放在大氣中(揮散)之步驟(即，前述(1)及(2)的步驟)者，即無特別限制使用之器具等。 The tolerance enhancement method of the present invention is not particularly limited to the apparatus used as long as it includes the steps of mixing the active ingredient compound of the present invention and triethyl citrate, preparing a composition containing the active ingredient compound (the composition of the present invention), and releasing the active ingredient compound of the present invention into the atmosphere (evaporation) (i.e., the steps (1) and (2) above).

本發明的耐受性增強方法，具體上係例如以前述(1)的步驟調製本發明的組成物後，在含有昇華性物質的非活性載體上均等的散布或滴下一定量(飽和承載量)的本發明之組成物，充分混合之後，視需要而使用錘式粉碎機粉碎，並以壓片機、壓塊機、造粒機等成形機加壓成形，製造含有本發明的組成物之錠劑。將該錠劑放入容器中，將其放置在栽培設施中使用，或懸掛在栽培設施的支 柱、植物株的上方等使用，藉此可將本發明的有效成分化合物釋放到大氣中。此外，在純淨水中攪拌凝膠化劑，使其懸浮，高壓釜處理(大約120℃、5分鐘)後，冷卻至約40℃，加入界面活性劑製作凝膠基質，將經前述(1)的步驟調製之本發明的組成物和非活性載體放入容器中，並倒入前述凝膠基質，攪拌、混合後，靜置至使凝膠固化，製造含有本發明的組成物之半固形製劑(凝膠狀製劑)，將該半固形製劑(凝膠狀製劑)放入容器中，將其放置在栽培設施中使用，或懸掛在栽培設施的支柱、植物株的上方等使用，即可將本發明的有效成分化合物釋放到大氣中。 The tolerance enhancement method of the present invention is specifically, for example, after preparing the composition of the present invention in the step (1) above, a certain amount (saturated loading amount) of the composition of the present invention is evenly spread or dropped on an inactive carrier containing a sublimable substance, and after sufficient mixing, it is crushed using a hammer mill as needed, and pressed and formed using a molding machine such as a tablet press, a briquette press, a granulator, etc. to produce a tablet containing the composition of the present invention. The tablet is placed in a container and placed in a cultivation facility for use, or hung on a support column of the cultivation facility, above a plant strain, etc., so that the active ingredient compound of the present invention can be released into the atmosphere. In addition, the gelling agent is stirred in pure water to make it suspended, and after autoclaving (about 120°C, 5 minutes), it is cooled to about 40°C, and a surfactant is added to make a gel matrix. The composition of the present invention prepared in the above step (1) and the inactive carrier are placed in a container, and the above gel matrix is poured in. After stirring and mixing, it is left to stand until the gel solidifies to make a semi-solid preparation (gel-like preparation) containing the composition of the present invention. The semi-solid preparation (gel-like preparation) is placed in a container and placed in a cultivation facility for use, or hung on the pillars of the cultivation facility, above the plant strain, etc., and the active ingredient compound of the present invention can be released into the atmosphere.

用以收納含有本發明的組成物之上述錠劑或半固形製劑(凝膠狀製劑)的容器，並無任何的限制，可使用該技術領域傳統常用的技術，但該容器係以具有為懸掛在栽培設施的支柱、植物株的上方等使用之掛鈎，或掛在植物株的上方等用之特定孔部(非通氣用)為佳。 The container for storing the tablet or semi-solid preparation (gel preparation) containing the composition of the present invention is not limited in any way, and conventional techniques in the technical field can be used. However, the container is preferably provided with a hook for hanging on a pillar of a cultivation facility, above a plant strain, etc., or a specific hole (not for ventilation) for hanging above a plant strain, etc.

該容器的形狀並無特別的限制，只要是可收納本發明的組成物，且在一部分形成開口部(包含小孔部)的形狀，或可使本發明的組成物中之本發明的有效成分化合物及昇華性物質滲透(揮散)的形狀即可，以袋狀為佳。 The shape of the container is not particularly limited, as long as it can contain the composition of the present invention and form an opening (including a small hole) in a part, or can allow the active ingredient compound of the present invention and the sublimable substance in the composition of the present invention to permeate (volatilize), preferably a bag shape.

該容器的一部分覆蓋有透明樹脂製的膜(例如口部、側窗)，以便容易以目視確認所收納的本發明之組成物的減少或消失。 A portion of the container is covered with a transparent resin film (e.g., the mouth, side window) to facilitate visual confirmation of the reduction or disappearance of the contained composition of the present invention.

此透明的樹脂製膜可列舉例如：將聚丙烯(PP)、聚乙烯(PE)、氯乙烯、丙烯腈‧丁二烯‧苯乙烯共聚物(ABS)、4-甲基-1-戊烯作成基質的聚烯烴(商品名「TPX」，三井化學股份有限公司製造)，此外，透明的多層膜可列舉例如將聚丙烯的多層片(商品名「Multilay」，出光興產股份有限公司製造)、丙烯腈系可塑性樹脂作成主體的多層片(商品名「Zekrom」，三井化學股份有限公司製造)等。 Examples of such transparent resin films include polypropylene (PP), polyethylene (PE), vinyl chloride, acrylonitrile-butadiene-styrene copolymer (ABS), and polyolefins with 4-methyl-1-pentene as the base (trade name "TPX", manufactured by Mitsui Chemicals Co., Ltd.). In addition, examples of transparent multilayer films include multilayer sheets of polypropylene (trade name "Multilay", manufactured by Idemitsu Kosan Co., Ltd.) and multilayer sheets with acrylonitrile-based plastic resin as the main body (trade name "Zekrom", manufactured by Mitsui Chemicals Co., Ltd.).

該容器的較佳型態，可舉出將本發明的組成物中之本發明的有效成分化合物及昇華性物質封入不滲透的包裝材中，使用時打開將部分包裝材，或以特定比例形成通氣用的特定孔者。 The preferred form of the container is to seal the active ingredient compound and sublimable substance of the present invention in an impermeable packaging material, and to open part of the packaging material when in use, or to form specific holes for ventilation in a specific ratio.

該包裝材的材質並無特別的限制，可列舉例如：玻璃、陶瓷、金屬、塑膠等。使用塑膠製的包裝材時，必需是不會使本發明的組成物中之本發明的有效成分化合物及昇華性物質滲透的材質，此材質可列舉例如：聚酯(PET、PBT等)、聚丙烯腈、聚縮醛、聚碳酸酯、聚醯胺等。 There is no particular restriction on the material of the packaging material, and examples thereof include glass, ceramics, metal, plastic, etc. When using a plastic packaging material, it must be a material that does not allow the active ingredient compound and sublimable substance of the present invention in the composition of the present invention to penetrate, and examples of such materials include polyester (PET, PBT, etc.), polyacrylonitrile, polyacetal, polycarbonate, polyamide, etc.

該容器的更佳型態可舉出具有阻氣性中間層的容器(以袋子為佳)。其中，係以經具有本發明的組成物中之本發明的有效成分化合物及昇華性物質不滲透的阻氣性無機物(鋁箔或氧化矽蒸鍍)被覆之中間層的可撓性袋狀軟包材為佳，以鋁蒸鍍積層膜之袋更佳。 A better form of the container is a container (preferably a bag) having a gas barrier intermediate layer. Among them, a flexible bag-shaped soft packaging material having an intermediate layer coated with a gas barrier inorganic material (aluminum foil or silicon oxide vapor deposition) that is impermeable to the active ingredient compound of the present invention and the sublimable substance in the composition of the present invention is preferred, and a bag with aluminum vapor deposition laminate film is more preferred.

該容器的另一較佳型態可列舉例如：以可撓性袋的兩面或一面係經使該組成物中的液狀成分及昇華性物質滲透之藥劑滲透性膜內面積層而成的和紙或不織布，或係經積層展延法製造的長纖維不織布，如單面時，另一面係該組成物中的液狀成分及昇華性物質不滲透之阻氣性膜(藥劑不滲透性膜)形成的袋狀容器。上述藥劑不滲透性膜可列舉例如：聚酯(PET、PBT等)、聚丙烯腈、聚縮醛、聚碳酸酯、聚醯胺等，藥劑不滲透性膜可列舉例如：聚乙烯(PE)或聚丙烯(PP)等聚烯烴、乙烯乙酸乙烯酯(EVA)等。本型態中，係不需在容器的一部分設置開口部(包括小孔部)，將內含本發明的組成物之上述袋狀容器再收納在不滲透液劑的外袋中，密封保存、販售，使用時從該外袋中取出上述袋狀容器，就可直接使用。此容器的具體例可列舉例如：國際公開第2021/186778號所述之容器等。 Another preferred form of the container is, for example, a bag-shaped container in which both sides or one side of a flexible bag are formed by layering a Japanese paper or nonwoven fabric with a drug-permeable membrane that allows the liquid components and sublimable substances in the composition to permeate, or a long-fiber nonwoven fabric manufactured by a multilayer stretching method. If it is a single-sided bag, the other side is formed by a gas-barrier membrane (drug-impermeable membrane) that prevents the liquid components and sublimable substances in the composition from permeating. The above-mentioned drug-impermeable membrane can be, for example, polyester (PET, PBT, etc.), polyacrylonitrile, polyacetal, polycarbonate, polyamide, etc., and the drug-impermeable membrane can be, for example, polyolefins such as polyethylene (PE) or polypropylene (PP), ethylene vinyl acetate (EVA), etc. In this form, it is not necessary to set an opening (including a small hole) in a part of the container. The above-mentioned bag-shaped container containing the composition of the present invention is further stored in an outer bag of an impermeable liquid, sealed and stored, and sold. When used, the above-mentioned bag-shaped container can be taken out from the outer bag and used directly. Specific examples of this container can be, for example, the container described in International Publication No. 2021/186778, etc.

此外，本發明的耐受性增強方法的更佳具體例，可列舉使用以下例示之耐受性增強劑的產器具之方法。從減輕從事農業者的負擔(各成分的準備、稱重的功夫、製劑化的步驟、手指的污染或傷害等)、保持作用效果的再現性等觀點，係以使用下述耐受性增強劑的產生器為佳。 In addition, a more specific example of the tolerance enhancement method of the present invention can be a method using the following tolerance enhancer production device. From the perspective of reducing the burden on farmers (preparation of each component, weighing time, preparation steps, finger contamination or damage, etc.) and maintaining the reproducibility of the effect, it is better to use the following tolerance enhancer production device.

本發明的耐受性增強方法，係以使用以下例示的耐受性增強劑的產生器之方法為佳。藉由使用耐受性增強劑的產生器(1)至(5)，即可用簡便的操作使含有分離狀態的本發明之有效成分化合物和檸檬酸三乙酯之液劑混合而形成組成物，藉此即可穩定本發明的有效成分化合物，並持續的釋放在大氣中，可長期持續的增強或改善植物的耐熱性及耐乾燥性。 The tolerance enhancing method of the present invention is preferably a method using the tolerance enhancing agent generator exemplified below. By using the tolerance enhancing agent generators (1) to (5), a liquid containing the separated active ingredient compound of the present invention and triethyl citrate can be mixed to form a composition by simple operation, thereby stabilizing the active ingredient compound of the present invention and continuously releasing it into the atmosphere, thereby continuously enhancing or improving the heat resistance and desiccation resistance of plants for a long time.

(本發明的耐受性增強劑之產生器) (Generator of the tolerance enhancer of the present invention)

本發明的耐受性增強方法之前述(1)及(2)的步驟中可使用的耐受性增強劑之產生器的較佳型態之一例，可列舉一種耐受性增強劑的產生器(本發明的耐受性增強劑的產生器(1))，其特徵為，在可設置一個以上的散發孔之可撓性容器內收納至少一個易碎性容器，該易碎性容器係含有選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物(本發明的有效成分化合物)(內部液)，前述可撓性容器中具有散發孔時，將散發孔密封，或無散發孔時，則在末端配置吸收性材料，且在易碎性容器的外側且可撓性容器內收納含有檸檬酸三乙酯的液劑(外部液體)。前述可撓性容器中收納的含有前述內部液之易碎性容器，可以係一個，也可以係二個以上。 As an example of a preferred form of a tolerance enhancer generator that can be used in the steps (1) and (2) of the tolerance enhancement method of the present invention, there can be cited a tolerance enhancer generator (the tolerance enhancer generator (1) of the present invention), which is characterized in that at least one fragile container is contained in a flexible container that can be provided with one or more diffusion holes, and the fragile container contains a compound selected from α , β -enal, α , β -enone, γ , δ -enal, γ , δ -enone, δ , ε -enal, δ , ε -enone, ε , ζ -enal and ε , ζ - unsaturated carbonyl compound with 4 to 9 carbon atoms and no unsaturated bond at the terminal of at least one of the group consisting of alkenone (effective ingredient compound of the present invention) (inner liquid), when the flexible container has diffusion holes, the diffusion holes are sealed, or when there are no diffusion holes, an absorbent material is arranged at the terminal, and a liquid containing triethyl citrate (external liquid) is stored outside the frangible container and inside the flexible container. The frangible container containing the inner liquid stored in the flexible container may be one or more than two.

耐受性增強劑的產生器之另一較佳的型態，可列舉一種耐受性增強劑的產生器(本發明的耐受性增強劑的產生器(2))，其特徵為，在可設置一個以 上的散發孔之可撓性容器內收納第1易碎容器，該第1易碎容器係含有選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物(本發明的有效成分化合物)(內部液1)，前述可撓性容器中具有散發孔時，將散發孔密封，或無散發孔時，則在末端配置吸收性材料，且在第1易碎容器的外側且可撓性容器內收納含有檸檬酸三乙酯的液劑(外部液體)。 Another preferred form of a tolerance enhancer generator is a tolerance enhancer generator (the tolerance enhancer generator (2) of the present invention), characterized in that a first fragile container is contained in a flexible container that can be provided with one or more diffusion holes, and the first fragile container contains a compound selected from α , β -enal, α , β -enone, γ , δ -enal, γ , δ -enone, δ , ε -enal, δ , ε -enone, ε , ζ -enal and ε , ζ-enone . -enone, wherein at least one of the group consisting of an unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal (the active ingredient compound of the present invention) (inner liquid 1); when the flexible container has diffusion holes, the diffusion holes are sealed; when there are no diffusion holes, an absorbent material is arranged at the terminal, and a liquid containing triethyl citrate (external liquid) is contained outside the first frangible container and in the flexible container.

此外，作為本發明的耐受性增強劑的產生器(2)之另一型態，前述可撓性容器的內部除了含有本發明的有效成分化合物(內部液1)之第1易碎性容器及含有檸檬酸三乙酯的液劑(內部液2)之第2易碎性容器以外，也可再收納含有前述併用劑及/或其他的添加劑(內部液3)之第3易碎性容器。 In addition, as another form of the tolerance enhancer generator (2) of the present invention, the flexible container may contain a third frangible container containing the aforementioned concomitant agent and/or other additives (inner liquid 3) in addition to the first frangible container containing the active ingredient compound of the present invention (inner liquid 1) and the second frangible container containing the liquid of triethyl citrate (inner liquid 2).

另外，作為本發明的耐受性增強劑的產生器(2)之另一型態，可以係在前述可撓性容器的內部收納至少一個易碎性容器(本發明的耐受性增強劑的產生器(2’))，該易碎性容器係包含預先調製的含有本發明之有效成分化合物和檸檬酸三乙酯之組成物，以取代將內部液1和內部液2置入各個易碎性容器而收納。 In addition, as another form of the tolerance enhancer generator (2) of the present invention, at least one fragile container (the tolerance enhancer generator (2') of the present invention) can be stored inside the aforementioned flexible container, and the fragile container contains a pre-prepared composition containing the active ingredient compound of the present invention and triethyl citrate, instead of placing the inner liquid 1 and the inner liquid 2 in each fragile container for storage.

此外，耐受性增強劑的產生器之另一較佳型態，可使用日本特開2017-6100號公報所述之器具。此種本發明的耐受性增強劑的產生器之一例，可列舉一種耐受性增強劑的產生器(本發明的耐受性增強劑的產生器(3))，其特微為，該耐受性增強劑的產生器具備：頂部密封、底部開放的圓筒狀可撓性容器，***於前述可撓性容器內部的收納空間之含有選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合 物(本發明的有效成分化合物)(內部液)的至少一個易碎容器，附加在前述可撓性容器的底部之附件，及安裝在前述附件和前述可撓性容器之間的滲透膜，其中，前述附件具有與前述可撓性容器之內面相對向的側面及與前述可撓性容器之底部相對向的上表面，前述附件在前述表面具有貫穿孔，同時在前述側面具有一個或多個空隙，且在前述易碎容器的外側且可撓性容器內收納含有檸檬酸三乙酯的液劑(外部液體)。本發明的耐受性增強劑的產生器(3)因係在底部附接附件，故可防止本發明的組成物漏出。收納在前述可撓性容器中的含有前述內部液之易碎性容器，可以係一個，也可以係二個以上。 In addition, another preferred form of the tolerance enhancer generator can use the apparatus described in Japanese Patent Publication No. 2017-6100. An example of such a tolerance enhancer generator of the present invention is a tolerance enhancer generator (the tolerance enhancer generator (3) of the present invention), wherein the tolerance enhancer generator comprises: a cylindrical flexible container with a sealed top and an open bottom, and a storage space inserted into the flexible container containing a substance selected from α , β -enal, α , β -enone, γ , δ-enal, γ , δ -enone, δ , ε -enal, δ , ε -enone, ε , ζ - enal and ε , ζ At least one frangible container containing at least one unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal of the group consisting of cyclopentane and cyclopentane (the active ingredient compound of the present invention) (internal liquid), an attachment attached to the bottom of the flexible container, and a permeable membrane installed between the attachment and the flexible container, wherein the attachment has a side surface opposite to the inner surface of the flexible container and an upper surface opposite to the bottom of the flexible container, the attachment has a through hole on the surface and one or more gaps on the side surface, and a liquid containing triethyl citrate (external liquid) is stored outside the frangible container and inside the flexible container. The generator (3) of the tolerance enhancer of the present invention can prevent the composition of the present invention from leaking out because the attachment is attached to the bottom. The number of the frangible container containing the internal liquid housed in the flexible container may be one or more.

此外，作為本發明的耐受性增強劑的產生器(3)之另一型態，可列舉一種耐受性增強劑的產生器(本發明的耐受性增強劑的產生器(4))，其特徵為，在前述可撓性容器的內部之收納空間，除了含有本發明的有效成分化合物(內部液1)之易碎性容器(第1易碎性容器)以外，也收納裝有含有檸檬酸三乙酯的液劑(內部液2)之另一易碎性容器(第2易碎性容器)，以取代在前述易碎性容器的外側之可撓性容器內收納檸檬酸三乙酯可撓性容器。另外，作為本發明的耐受性增強劑的產生器(4)之另一型態，也可再收納裝有前述併用劑及/或其他的添加劑之液劑(內部液3)之另外的易碎容器(第3破割性容器)。 In addition, as another type of the tolerance enhancer generator (3) of the present invention, there can be cited a tolerance enhancer generator (the tolerance enhancer generator (4) of the present invention), which is characterized in that, in addition to the frangible container (first frangible container) containing the active ingredient compound (inner liquid 1) of the present invention, another frangible container (second frangible container) containing a liquid containing triethyl citrate (inner liquid 2) is also stored in the storage space inside the aforementioned flexible container, instead of storing the triethyl citrate flexible container in the flexible container outside the aforementioned frangible container. In addition, as another form of the tolerance enhancer generator (4) of the present invention, another fragile container (third fracturable container) containing the aforementioned concomitant and/or other additive liquid (internal liquid 3) can also be stored.

此外，作為本發明的耐受性增強劑的產生器(4)之其他型態，可列舉一種耐受性增強劑的產生器(本發明的耐受性增強劑的產生器(4’))，其特徵為，在前述可撓性容器的內部之收納空間，收納包含預先調製的含有本發明之有效成分化合物和檸檬酸三乙酯的組成物之至少一個易碎性容器，以取代將內部液1和內部液2裝入各個易碎性容器而收納。 In addition, as another form of the tolerance enhancer generator (4) of the present invention, a tolerance enhancer generator (the tolerance enhancer generator (4') of the present invention) can be cited, which is characterized in that at least one frangible container containing a pre-prepared composition containing the active ingredient compound of the present invention and triethyl citrate is stored in the storage space inside the aforementioned flexible container, instead of storing the internal liquid 1 and the internal liquid 2 in separate frangible containers.

作為本發明的耐受性增強劑的產生器(3)、(4)及(4’)的又另外之較佳型態，可在前述可撓性容器和前述滲透膜之間再設置具有至少一個開口部的支撐具(以下，亦稱「本發明的耐受性增強劑的產生器(5)」)。此支撐具在未使用本發明的耐受性增強劑的產生器(5)時的保管中或使用期間破裂時，負有保護滲透膜的作用，以防易碎性容器及其碎片直接接觸滲透膜而損壞滲透膜，同時做成具有可通過本發明的組成物之小孔(開口部)的結構。此支撐具的材質並無特別的限制，可適合使用選自由聚丙烯、聚乙烯、聚胺酯、聚醯胺(例如尼龍)及聚氯乙烯所組成之群組中的樹脂製者。 As another preferred form of the tolerance enhancer generator (3), (4) and (4') of the present invention, a support having at least one opening (hereinafter, also referred to as "the tolerance enhancer generator (5) of the present invention") may be provided between the aforementioned flexible container and the aforementioned permeable membrane. This support has the function of protecting the permeable membrane during storage when the tolerance enhancer generator (5) of the present invention is not in use or when it breaks during use, so as to prevent the fragile container and its fragments from directly contacting the permeable membrane and damaging the permeable membrane, and at the same time, is formed into a structure having a small hole (opening) through which the composition of the present invention can pass. The material of the support is not particularly limited, and it can be made of a resin selected from the group consisting of polypropylene, polyethylene, polyurethane, polyamide (such as nylon) and polyvinyl chloride.

本發明的耐受性增強劑的產生器(1)、(3)及(5)中，因收納在可撓性容器中的外部液(含有檸檬酸三乙酯之液劑)係充當為緩衝材，故具有可防止運送(未使用時)該耐受性增強劑的產生器(1)、(3)及(5)因搖晃導致易碎性容器(玻璃安瓿)破裂的優點。 In the tolerance enhancer generators (1), (3) and (5) of the present invention, since the external liquid (liquid containing triethyl citrate) contained in the flexible container acts as a buffer, it has the advantage of preventing the fragile container (glass ampoule) from breaking due to shaking during transportation (when not in use) of the tolerance enhancer generators (1), (3) and (5).

本發明的耐受性增強劑的產生器中之可撓性容器，可以係透明，可以係不透明，也可以係上色者。該可撓性容器的材質只要係可撓性材質即無特別的限制，可適合使用例如聚丙烯、聚乙烯、聚胺酯、聚醯胺(例如尼龍)、聚氯乙烯等樹脂製之材質。作為該可撓性容器，可如圖1至3中所述之細長圓筒狀之容器(棒狀容器)，但並不限於其形狀者。該容器中，可如圖1至3所示的在一處設置散發孔(或開口部)，也可在容器表面的多處設置。本發明的有效成分化合物係通過散發孔(或開口部)或從樹脂製的可撓性容器之上表面釋放至大氣中。 The flexible container in the generator of the tolerance enhancer of the present invention can be transparent, opaque, or colored. The material of the flexible container is not particularly limited as long as it is a flexible material, and materials made of resins such as polypropylene, polyethylene, polyurethane, polyamide (such as nylon), and polyvinyl chloride can be used. As the flexible container, it can be a slender cylindrical container (rod-shaped container) as described in Figures 1 to 3, but it is not limited to its shape. In the container, a diffusion hole (or opening) can be set at one place as shown in Figures 1 to 3, or it can be set at multiple places on the surface of the container. The active ingredient compound of the present invention is released into the atmosphere through the diffusion hole (or opening) or from the upper surface of the flexible container made of resin.

本發明的耐受性增強劑的產生器中的易碎性容器係玻璃容器，尤其以玻璃安瓿特別佳。該易碎性容器係玻璃製時，使用可用手指力就破裂的薄質容器。此玻璃的厚度係以0.1至0.4mm特別佳。藉由使用玻璃安瓿作為該易碎 性容器，即可在含有作為本發明的有效成分之揮發性的前述碳數4至9之不飽和羰基化合物的狀態中，不損壞品質的長期穩定保存。 The fragile container in the generator of the tolerance enhancer of the present invention is a glass container, and a glass ampoule is particularly preferred. When the fragile container is made of glass, a thin container that can be broken by finger force is used. The thickness of the glass is particularly preferably 0.1 to 0.4 mm. By using a glass ampoule as the fragile container, the aforementioned unsaturated carbonyl compound with 4 to 9 carbon atoms, which is a volatile active ingredient of the present invention, can be stored stably for a long time without deteriorating the quality.

本發明的耐受性增強劑的產生器(1)、(2)及(2’)中之吸收性材料，係指由天然或合成纖維質、或多孔質所構成之材料，具有暫時吸附有效成分等的功能和防止玻璃碎片飛出之功能。該吸收性材料係如圖1中所示，可安裝在散發孔附近，也可在易碎性容器(玻璃安瓿)的周圍以捲繞的方式安裝。 The absorbent material in the tolerance enhancer generator (1), (2) and (2') of the present invention refers to a material composed of natural or synthetic fibers or porous materials, which has the function of temporarily absorbing active ingredients and preventing glass fragments from flying out. The absorbent material can be installed near the diffusion hole as shown in Figure 1, or can be installed in a winding manner around a fragile container (glass ampoule).

本發明的耐受性增強劑的產生器(3)、(4)、(4’)及(5)中的滲透膜，係指可使氣化的有效成分滲透之膜，滲透性能可配合本發明的有效成分化合物之特性選擇材質而調節。滲透膜係以熱焊接或聲波焊接使其固定在該產生器(3)、(4)、(4’)及(5)的底部外緣上為佳。滲透膜的材質並無特別的限制，可以係和可撓性容器的材質相同之薄膜，藉此確實的焊接及固定。 The permeable membrane in the generators (3), (4), (4') and (5) of the tolerance enhancer of the present invention refers to a membrane that allows the vaporized active ingredient to permeate, and the permeability can be adjusted by selecting the material in accordance with the characteristics of the active ingredient compound of the present invention. The permeable membrane is preferably fixed to the outer edge of the bottom of the generator (3), (4), (4') and (5) by heat welding or ultrasonic welding. The material of the permeable membrane is not particularly limited, and it can be a thin film of the same material as the flexible container, thereby ensuring welding and fixing.

本發明的耐受性增強劑的產生器(3)、(4)、(4’)及(5)中的附件，係安裝在如同***底部開放的細長圓筒狀的可撓性容器之底面上。該附件的外緣大小係對應於前述可撓性容器的底部之內徑。該附件具有與可撓性容器的內面相對向之側面及與可撓性容器的底部相對向之上表面，在前述側面上具有多個空隙。附件配備的前述貫穿孔、前述空隙係介由滲透膜而使前述可撓性容器和外部連接。因此，本發明的耐受性增強劑的產生器(3)、(4)及(5)中，從含有本發明的有效成分化合物(內部液)之易碎性容器(玻璃安瓿)中釋放出的有效成分，係和前述可撓性容器中含有檸檬酸三乙酯之液劑(外部液)、或從裝入含有檸檬酸三乙酯之液劑(內部液2)的易碎性容器(玻璃安瓿)中釋放的檸檬酸三乙酯混合而形成組成物，該組成物係積聚在滲透膜的內部且可撓性容器和附件之間。此積聚的組成物雖然可經滲透膜而停留在前述可撓性容器中，但也可經滲透膜的滲透性能 而揮發(釋放)至大氣中。附件的材質並無特別的限制，係以和可撓性容器的材質相同較佳。 The attachments in the tolerance enhancer generators (3), (4), (4') and (5) of the present invention are installed on the bottom surface of a flexible container that is inserted into an elongated cylindrical shape with an open bottom. The outer edge size of the attachment corresponds to the inner diameter of the bottom of the flexible container. The attachment has a side surface opposite to the inner surface of the flexible container and an upper surface opposite to the bottom of the flexible container, and has a plurality of gaps on the side surface. The through holes and gaps provided in the attachment connect the flexible container to the outside through a permeable membrane. Therefore, in the generators (3), (4) and (5) of the tolerance enhancer of the present invention, the active ingredient released from the frangible container (glass ampoule) containing the active ingredient compound of the present invention (internal liquid) is mixed with the liquid containing triethyl citrate (external liquid) in the aforementioned flexible container, or triethyl citrate released from the frangible container (glass ampoule) filled with the liquid containing triethyl citrate (internal liquid 2) to form a composition, and the composition is accumulated inside the permeable membrane and between the flexible container and the attachment. Although this accumulated composition can pass through the permeable membrane and stay in the aforementioned flexible container, it can also volatilize (release) into the atmosphere through the permeability of the permeable membrane. There is no particular restriction on the material of the accessories, but it is best to use the same material as the flexible container.

本發明的耐受性增強劑的產生器係以具有為使用而吊掛在栽培設施(例如塑膠型溫室等)的支柱、植物株的頂部等上的掛鈎，或吊掛在植物株的頂部用之特殊孔部(非前述的散發孔)為佳。 The tolerance enhancer generator of the present invention preferably has a hook for hanging on a support of a cultivation facility (such as a plastic greenhouse, etc.), the top of a plant, etc., or a special hole (not the aforementioned diffusion hole) for hanging on the top of a plant.

在使用本發明的耐受性增強劑的產生器時，用手指的力量折彎可撓性容器使其中的易碎性容器(玻璃安瓿)破裂，使含有分離狀態的本發明之有效成分化合物和檸檬酸三乙酯的液劑混合而形成組成物，使該組成物穿透吸收性材料而從容器的發散孔或樹脂製的可撓性容器之表面散發(揮發)至大氣中。 When using the tolerance enhancer generator of the present invention, the flexible container is bent with the strength of the fingers to break the fragile container (glass ampoule) therein, so that the liquid containing the active ingredient compound of the present invention and triethyl citrate in a separated state is mixed to form a composition, and the composition is allowed to penetrate the absorbent material and diffuse (volatize) into the atmosphere from the diffusion holes of the container or the surface of the flexible container made of resin.

本發明的耐受性增強劑的產生器之製造方法並無特別的限制，較佳的係例如在圓筒狀可撓性容器(長度20mm至200mm、外徑3mm至12mm、質厚0.2mm至3mm)的一端或兩端設置小發散孔。用吸收性材料填充發散孔內部。易碎性容器係玻璃安瓿製，且沿著容器作成細長形狀，熔封口係尖的。可用縮小安瓿尖端的開口部，以將含有本發明的有效成分化合物等揮發性成分之液體充填熔封至安瓿中，裝入有效成分等之後，瞬間將開口部熱熔並熔封的方法製造。 There is no particular limitation on the manufacturing method of the tolerance enhancer generator of the present invention. A preferred method is, for example, to set a small diffusion hole at one or both ends of a cylindrical flexible container (length 20mm to 200mm, outer diameter 3mm to 12mm, thickness 0.2mm to 3mm). Fill the inside of the diffusion hole with an absorbent material. The fragile container is made of a glass ampoule, and is made into a slender shape along the container, and the sealing mouth is pointed. The opening of the tip of the ampoule can be reduced to fill and seal the liquid containing the volatile components such as the active ingredient compound of the present invention into the ampoule, and after the active ingredient is loaded, the opening is instantly hot-melted and sealed.

藉由使用本發明的耐受性增強劑的產生器(1)、(2)、(2’)、(3)、(4)、(4’)及(5)的任一種，可使從事農業者無負擔(各成分的準備、稱重的功夫、製劑化的步驟、手指的污染或傷害等)地有效保持本發明的耐受性增強劑之品質和作用效果之再現性。 By using any one of the tolerance enhancer generators (1), (2), (2'), (3), (4), (4') and (5) of the present invention, farmers can effectively maintain the quality and reproducibility of the tolerance enhancer of the present invention without the burden (preparation of each component, weighing time, preparation steps, finger contamination or damage, etc.).

本發明的有效成分化合物揮發後，較佳係將本發明的耐受性增強劑的產生器拋棄並替換成新的產生器，但因本發明的耐受性增強劑的產生器(3)、 (4)、(4’)及(5)係可裝卸者，故也可僅拋棄本發明的有效成分化合物揮發後之殘留液，並補充另外調製的本發明之組成物再使用。 After the active ingredient compound of the present invention evaporates, it is preferred to discard the generator of the tolerance enhancer of the present invention and replace it with a new one. However, since the generators (3), (4), (4') and (5) of the tolerance enhancer of the present invention are detachable, it is also possible to only discard the residual liquid after the active ingredient compound of the present invention evaporates and replenish it with the composition of the present invention prepared separately for reuse.

又，本發明並非侷限於上述的各實施型態及實施例中之範圍者，可作成各種的變更，並適當的組合不同的實施型態中各別揭示的技術方式而得的實施型態，也包含在本發明的技術範圍內。此外，本說明書中所述的所有先前技術文獻，均在本說明書中作為參考而引用。 Furthermore, the present invention is not limited to the above-mentioned embodiments and the scope of the embodiments, and various modifications can be made, and the embodiments obtained by appropriately combining the technical methods disclosed in different embodiments are also included in the technical scope of the present invention. In addition, all the previous technical documents described in this specification are cited as references in this specification.

[實施例] [Implementation example]

以下，以實施例及試驗例詳細說明本發明，但本發明並非侷限於此等的範圍者。又，以下的「%」係指「重量%」。 The present invention is described in detail below with examples and test examples, but the present invention is not limited to these scopes. In addition, the "%" below refers to "weight %".

下述實施例、比較例及試驗例中使用的化合物之2-己烯醛(商品名：LAL(反式-2-己烯醛)；JNC股份有限公司製造)、檸檬酸三乙酯(商品名：Citroflex 2(註冊商標)，森村商事股份有限公司製造)、2,4,6-三第三丁基-1,3,5-三噁烷(商品名：Sunsaburi B(以下可簡稱有時縮寫為「SSB」)，小川香料股份有限公司製造)、Floralette(註冊商標)(直徑約23mm、厚度約8mm、重量1.35g)(LETS ONE股份有限公司製造)、生物分解性球狀纖維素(商品名：Visco Pearl(註冊商標)，Rengo股份有限公司製造)、橄欖油(商品名：特級初榨橄欖油，加西亞德拉克魯斯(Garcia de la Cruz)公司製造)、亞麻仁油(商品名：日清亞麻仁油，日清奧利裡奧集團(Nisshin Oilio Group)股份有限公司製造)、紅花油(商品名：味之素(AJINOMOTO)紅花油；味之素股份有限公司製造)、二-第三丁基-對-甲酚(DBPC)(東京化成工業股份有限公司製造)、紫外線吸收劑(主成分：氧化鋅55%)(商品名：DIF-AB-33w，堺化學工業股份有限公司製造)、α-生育酚(tocopherol)(富士軟片和光純藥股份有限公司製造)、紫外線吸收劑(主成分：氧化 鋅55%)(商品名：DIF-3W4，堺化學工業股份有限公司製造)、異烷烴系高純度溶劑(商品名：IP溶劑2835，出光興產股份有限公司製造)、聚乙二醇(商品名：聚乙二醇400(PEG400)；富士軟片和光純藥股份有限公司製造)係分別直接使用市售品。 The compounds used in the following Examples, Comparative Examples and Test Examples include 2-hexenal (trade name: LAL (trans-2-hexenal); manufactured by JNC Co., Ltd.), triethyl citrate (trade name: Citroflex 2 (registered trademark), manufactured by Morimura Shoji Co., Ltd.), 2,4,6-tri-tert-butyl-1,3,5-trioxane (trade name: Sunsaburi B (hereinafter sometimes abbreviated as "SSB"), manufactured by Ogawa Fragrance Co., Ltd.), Floralette (registered trademark) (diameter about 23 mm, thickness about 8 mm, weight 1.35 g) (manufactured by LETS ONE Co., Ltd.), biodegradable spherical cellulose (trade name: Visco Pearl (registered trademark), manufactured by Rengo Co., Ltd.), olive oil (trade name: extra virgin olive oil, Garcia de la Cruz) de la Cruz), flax seed oil (trade name: Nissin flax seed oil, manufactured by Nisshin Oilio Group Co., Ltd.), safflower oil (trade name: Ajinomoto safflower oil; manufactured by Ajinomoto Co., Ltd.), di-tert-butyl-p-cresol (DBPC) (manufactured by Tokyo Chemical Industry Co., Ltd.), ultraviolet absorber (main component: zinc oxide 55%) (trade name: DIF-AB-33w, manufactured by Sakai Chemical Industry Co., Ltd.), α - Tocopherol (manufactured by Fujifilm Wako Pure Chemical Industries, Ltd.), ultraviolet absorber (main component: zinc oxide 55%) (trade name: DIF-3W4, manufactured by Sakai Chemical Industry Co., Ltd.), isoalkane-based high-purity solvent (trade name: IP solvent 2835, manufactured by Idemitsu Kosan Co., Ltd.), and polyethylene glycol (trade name: polyethylene glycol 400 (PEG400); manufactured by Fujifilm Wako Pure Chemical Industries, Ltd.) were commercially available products and used as they were.

本發明的有效成分化合物具有增強植物耐受性之效果已在前述專利文獻2(國際公開第2016/031775號)中有所敘述，因此在以下的實施例、比較例及試驗例中，係為研究其效果的持續性而使用2-己烯醛作為本發明的有效成分化合物，並研究在各種添加劑共存下或不共存下2-己烯醛隨時間的推移有無氣味及變質。 The active ingredient compound of the present invention has the effect of enhancing plant tolerance, which has been described in the aforementioned patent document 2 (International Publication No. 2016/031775). Therefore, in the following examples, comparative examples and test examples, 2-hexenal is used as the active ingredient compound of the present invention to study the sustainability of its effect, and to study whether 2-hexenal has odor and deterioration over time in the presence or absence of various additives.

〔調製試料〕 〔Preparation of samples〕

以下說明下述試驗例中使用的實施例及比較例之各試料的調製方法。 The following describes the preparation methods of the samples used in the following test examples and comparative examples.

〔實施例1〕 [Implementation Example 1]

圖1的產生器中，將在可撓性容器(1)內收納含有2-己烯醛(0.85g(1mL))作為內部液(3)的玻璃安瓿(2)，且收納檸檬酸三乙酯(3.42g(3mL))作為外部液者折彎，使玻璃安瓿(2)破裂之後，以懸掛在塑膠型溫室內的狀態進行下述試驗例。 In the generator of Figure 1, a glass ampoule (2) containing 2-hexenal (0.85 g (1 mL)) as an inner liquid (3) and triethyl citrate (3.42 g (3 mL)) as an outer liquid is stored in a flexible container (1). The glass ampoule (2) is folded to break, and then the ampoule is hung in a plastic greenhouse to perform the following test example.

〔實施例2〕 [Example 2]

圖2的產生器中，將在可撓性容器的收納空間(7)內收納含有2-己烯醛(0.85g(1mL))作為內部液(10)的玻璃安瓿(9)，並收納檸檬酸三乙酯(3.42g(3mL))作為外部液(8)者折彎，使玻璃安瓿(9)破裂之後，以懸掛在塑膠型溫室內的狀態進行下述試驗例。 In the generator of Figure 2, a glass ampoule (9) containing 2-hexenal (0.85 g (1 mL)) as an inner liquid (10) and triethyl citrate (3.42 g (3 mL)) as an outer liquid (8) is stored in the storage space (7) of a flexible container. The ampoule (9) is folded to break, and then the ampoule is suspended in a plastic greenhouse and the following test example is performed.

〔實施例3〕 [Implementation Example 3]

圖3的產生器中，將收納含有2-己烯醛(0.85g(1mL))作為內部液(19)的玻璃安瓿(18)，並收納檸檬酸三乙酯(3.42g(3mL))作為外部液(20)者折彎，使玻璃安瓿(18)破裂之後，以懸掛在塑膠型溫室內的狀態進行下述試驗例。 In the generator of Figure 3, a glass ampoule (18) containing 2-hexenal (0.85 g (1 mL)) as the inner liquid (19) and triethyl citrate (3.42 g (3 mL)) as the outer liquid (20) was bent to break the glass ampoule (18), and then the ampoule was suspended in a plastic greenhouse and the following test example was performed.

〔實施例4〕 [Implementation Example 4]

圖3的產生器中，將收納含有2-己烯醛(0.85g(1mL))作為內部液(19)的玻璃安瓿(18)，並收納檸檬酸三乙酯(1.71g(1.5mL))及PEG400(1.70g(1.5mL)作為外部液(20)者折彎，使玻璃安瓿(18)破裂之後，以懸掛在塑膠型溫室內的狀態進行下述試驗例。 In the generator of Figure 3, a glass ampoule (18) containing 2-hexenal (0.85 g (1 mL)) as the inner liquid (19) and containing triethyl citrate (1.71 g (1.5 mL)) and PEG400 (1.70 g (1.5 mL)) as the outer liquid (20) was bent to break the glass ampoule (18), and then the ampoule was suspended in a plastic greenhouse and the following test example was performed.

〔實施例5〕 [Example 5]

使Floralette(註冊商標)(LETS ONE股份有限公司製造)(1.35g)(直徑約23mm、厚度約8mm)中浸泡有2-己烯醛(0.5g)及檸檬酸三乙酯(0.5g)者放在盤上，在開放的狀態進行下述試驗例。 2-Hexenal (0.5 g) and triethyl citrate (0.5 g) were soaked in Floralette (registered trademark) (manufactured by LETS ONE Co., Ltd.) (1.35 g) (diameter about 23 mm, thickness about 8 mm) and placed on a plate, and the following test example was carried out in an open state.

〔實施例6〕 [Implementation Example 6]

將Floralette(註冊商標)(LETS ONE股份有限公司製造)(1.35g)(直徑約23mm、厚度約8mm)中浸泡有2-己烯醛(0.5g)及檸檬酸三乙酯(1.5g)者放在盤上，在開放的狀態進行下述試驗例。 Floralette (registered trademark) (manufactured by LETS ONE Co., Ltd.) (1.35 g) (diameter about 23 mm, thickness about 8 mm) soaked with 2-hexenal (0.5 g) and triethyl citrate (1.5 g) was placed on a plate and the following test was performed in an open state.

〔實施例7〕 [Implementation Example 7]

將Visco Pearl(註冊商標)(Rengo股份有限公司製造)(15cc)中浸泡有2-己烯醛(1.0g)及檸檬酸三乙酯(1.1g)者放入和國際公開第2021/186778號的圖1同樣的鋁蒸鍍積層膜的袋(外部尺寸100mm×100mm)中，在兩邊切開的狀態進行下述試驗例。 Visco Pearl (registered trademark) (manufactured by Rengo Co., Ltd.) (15cc) soaked with 2-hexenal (1.0g) and triethyl citrate (1.1g) was placed in an aluminum vapor-deposited laminate bag (external dimensions 100mm×100mm) similar to Figure 1 of International Publication No. 2021/186778, and the bag was cut open on both sides for the following test example.

〔比較例1〕 [Comparison Example 1]

圖1的產生器中，將在可撓性容器(1)內收納含有2-己烯醛(0.85g(1mL))作為內部液(3)的玻璃安瓿(2)，並收納橄欖油(2.73g(3mL))作為外部液者折彎，使玻璃安瓿(2)破裂之後，以懸掛在塑膠型溫室內的狀態進行下述試驗例。 In the generator of Figure 1, a glass ampoule (2) containing 2-hexenal (0.85 g (1 mL)) as the inner liquid (3) and olive oil (2.73 g (3 mL)) as the outer liquid is placed in a flexible container (1), and the ampoule (2) is folded to break, and then the ampoule (2) is suspended in a plastic greenhouse and the following test example is performed.

〔比較例2〕 [Comparison Example 2]

圖1的產生器中，將在可撓性容器(1)內收納含有2-己烯醛(0.85g(1mL))作為內部液(3)的玻璃安瓿(2)，並收納亞麻仁油(2.78g(3mL))作為外部液者折彎，使玻璃安瓿(2)破裂之後，以懸掛在塑膠型溫室內的狀態進行下述試驗例。 In the generator of Figure 1, a glass ampoule (2) containing 2-hexenal (0.85 g (1 mL)) as the inner liquid (3) and linseed oil (2.78 g (3 mL)) as the outer liquid is placed in a flexible container (1), and the ampoule (2) is bent to break, and then the ampoule (2) is suspended in a plastic greenhouse and the following test example is performed.

〔比較例3〕 [Comparison Example 3]

圖1的產生器中，將可撓性容器(1)內收納含有2-己烯醛(0.85g(1mL))作為內部液(3)的玻璃安瓿(2)，並收納紅花油(2.82g(3mL))作為外部液者折彎，使玻璃安瓿(2)破裂之後，以懸掛在塑膠型溫室內的狀態進行下述試驗例。 In the generator of Figure 1, a glass ampoule (2) containing 2-hexenal (0.85 g (1 mL)) as the inner liquid (3) and safflower oil (2.82 g (3 mL)) as the outer liquid is stored in a flexible container (1), and the glass ampoule (2) is folded to break, and then the container is suspended in a plastic greenhouse and the following test example is performed.

〔比較例4〕 [Comparison Example 4]

圖1的產生器中，將在可撓性容器(1)內收納含有2-己烯醛(0.85g(1mL))作為內部液(3)的玻璃安瓿(2)，並收納橄欖油(2.73g(3mL))及DBPC(0.014g)作為外部液者折彎，使玻璃安瓿(2)破裂之後，以懸掛在塑膠型溫室內的狀態進行下述試驗例。 In the generator of Figure 1, a glass ampoule (2) containing 2-hexenal (0.85 g (1 mL)) as the inner liquid (3) and olive oil (2.73 g (3 mL)) and DBPC (0.014 g) as the outer liquid is placed in a flexible container (1), and the ampoule (2) is folded to break, and then the ampoule (2) is suspended in a plastic greenhouse and the following test example is performed.

〔比較例5〕 [Comparison Example 5]

圖1的產生器中，將在可撓性容器(1)內收納含有2-己烯醛(0.85g(1mL))作為內部液(3)的玻璃安瓿(2)，並收納橄欖油(2.73g(3mL))及α-生育酚(0.014g)作為外部液者折彎，使玻璃安瓿(2)破裂之後，以懸掛在塑膠型溫室內的狀態進行下述試驗例。 In the generator of FIG1 , a glass ampoule (2) containing 2-hexenal (0.85 g (1 mL)) as an inner liquid (3) and olive oil (2.73 g (3 mL)) and α -tocopherol (0.014 g) as an outer liquid is contained in a flexible container (1). The glass ampoule (2) is folded to break, and then the ampoule is suspended in a plastic-type greenhouse and subjected to the following test example.

〔比較例6〕 [Comparative Example 6]

圖1的產生器中，將在可撓性容器(1)內收納含有2-己烯醛(0.85g(1mL))作為內部液(3)的玻璃安瓿(2)，並收納橄欖油(2.73g(3mL))作為外部液，且在可撓性容器(1)外側塗布紫外線吸收劑(DIF-AB-33w)者折彎，使玻璃安瓿(2)破裂之後，以懸掛在塑膠型溫室內的狀態進行下述試驗例。 In the generator of Figure 1, a glass ampoule (2) containing 2-hexenal (0.85 g (1 mL)) as the inner liquid (3) and olive oil (2.73 g (3 mL)) as the outer liquid is stored in a flexible container (1), and a UV absorber (DIF-AB-33w) is applied to the outer side of the flexible container (1). The flexible container (1) is bent to break the glass ampoule (2), and then the container is hung in a plastic greenhouse to perform the following test example.

〔比較例7〕 [Comparative Example 7]

圖1的產生器中，將在可撓性容器(1)內收納含有2-己烯醛(0.85g(1mL))作為內部液(3)的玻璃安瓿(2)，且收納橄欖油(2.73g(3mL))作為外部液，並在可撓性容器(1)外側塗布紫外線吸收劑(DIF-3w4)者折彎，使玻璃安瓿(2)破裂之後，以懸掛在塑膠型溫室內的狀態進行下述試驗例。 In the generator of Figure 1, a glass ampoule (2) containing 2-hexenal (0.85 g (1 mL)) as the inner liquid (3) and olive oil (2.73 g (3 mL)) as the outer liquid is stored in a flexible container (1). A UV absorber (DIF-3w4) is applied to the outer side of the flexible container (1), and the glass ampoule (2) is broken by bending the flexible container (1). The following test example is performed in a state where the flexible container (1) is hung in a plastic greenhouse.

〔比較例8〕 [Comparative Example 8]

圖1的產生器中，將在可撓性容器(1)內收納含有2-己烯醛(0.85g(1mL))作為內部液(3)的玻璃安瓿(2)，且收納PEG400((3.39g(3mL))作為外部液者折彎，使玻璃安瓿(2)破裂之後，以懸掛在塑膠型溫室內的狀態進行下述試驗例。 In the generator of Figure 1, a glass ampoule (2) containing 2-hexenal (0.85 g (1 mL)) as the inner liquid (3) and PEG400 (3.39 g (3 mL)) as the outer liquid is placed in a flexible container (1), and the ampoule (2) is folded to break, and then the ampoule is suspended in a plastic greenhouse and the following test example is performed.

〔比較例9〕 [Comparative Example 9]

圖3的產生器中，將收納含有2-己烯醛(0.85g(1mL))作為內部液(3)的玻璃安瓿(2)，且不含外部液(20)者折彎，使玻璃安瓿(18)破裂之後，以懸掛在塑膠型溫室內的狀態進行下述試驗例。 In the generator of Figure 3, a glass ampoule (2) containing 2-hexenal (0.85 g (1 mL)) as the inner liquid (3) and not containing the outer liquid (20) was bent to break the glass ampoule (18), and then the ampoule was suspended in a plastic greenhouse and the following test example was performed.

〔比較例10〕 〔Comparison Example 10〕

圖3的產生器中，將收納含有2-己烯醛(0.85g(1mL))作為內部液(19)的玻璃安瓿(18)，且收納IP溶劑2835(2.46g(3mL))作為外部液(20)者折彎，使玻璃安瓿(18)破裂之後，以懸掛在塑膠型溫室內的狀態進行下述試驗例。 In the generator of Figure 3, a glass ampoule (18) containing 2-hexenal (0.85 g (1 mL)) as the inner liquid (19) and IP solvent 2835 (2.46 g (3 mL)) as the outer liquid (20) was bent to break the glass ampoule (18), and then the ampoule was suspended in a plastic greenhouse and subjected to the following test example.

〔比較例11〕 〔Comparative Example 11〕

將Floralette(註冊商標)(LETS ONE股份有限公司製造)(1.35g)(直徑約23mm、厚度約8mm)中含浸有2-己烯醛(0.5g)者放在盤上，在開放的狀態進行下述試驗例。 Floralette (registered trademark) (manufactured by LETS ONE Co., Ltd.) (1.35 g) (diameter about 23 mm, thickness about 8 mm) impregnated with 2-hexenal (0.5 g) was placed on a plate and the following test example was performed in an open state.

〔比較例12〕 〔Comparative Example 12〕

相對於2,4,6-三第三丁基-1,3,5-三噁烷(SSB)(6.86g)，添加2-己烯醛(0.14g)並充分混合，以錠劑成形機(萊博尼科(Labnect)股份有限公司)加壓成形(25kN)，藉此得到長寬各30mm、厚度10.7mm、重量7g的錠劑。 2-Hexenal (0.14 g) was added to 2,4,6-tri-tert-butyl-1,3,5-trioxane (SSB) (6.86 g) and mixed thoroughly. The mixture was pressed (25 kN) using a tablet forming machine (Labnect Co., Ltd.) to obtain tablets with a length and width of 30 mm, a thickness of 10.7 mm, and a weight of 7 g.

將所得的錠劑裝入和國際公開第2021/186778號之圖1同樣的鋁蒸鍍積層膜之袋(外部尺寸100mm×100mm)中，在兩邊切開的狀態(開口部3cm)進行下述試驗例。 The obtained tablets were placed in an aluminum vapor-deposited laminated film bag (external dimensions 100 mm × 100 mm) similar to Figure 1 of International Publication No. 2021/186778, and the following test examples were performed with both sides cut open (opening 3 cm).

〔比較例13〕 〔Comparative Example 13〕

使Visco Pearl(註冊商標)(Rengo股份有限公司製造)(15cc)中浸泡有2-己烯醛(含0.5%DBPC(抗氧化劑)(2.1g)者放入和國際公開第2021/186778號之圖1同樣的鋁蒸鍍積層膜的袋(外部尺寸100mm×100mm)中，在兩邊切開(開口部3cm)的狀態進行下述試驗例。 Visco Pearl (registered trademark) (manufactured by Rengo Co., Ltd.) (15cc) soaked with 2-hexenal (containing 0.5% DBPC (antioxidant) (2.1g) was placed in an aluminum vapor-deposited laminated film bag (external dimensions 100mm×100mm) similar to Figure 1 of International Publication No. 2021/186778, and the bag was cut open on both sides (opening 3cm) to conduct the following test example.

〔試驗例1〕塑膠型溫室內的有效成分化合物(2-己烯醛)之揮散持續性的評價試驗 [Test Example 1] Evaluation test of the volatility persistence of the active ingredient compound (2-hexenal) in a plastic greenhouse

試驗係在2021年6月至9月及2022年5月至9月期間，於茨城縣結城郡八千代町的戶外防雨塑膠型溫室內進行。試驗期間塑膠型溫室內的最低氣溫係14.7℃，最高氣溫係41.1℃，平均氣溫係24.8℃。 The test was conducted in an outdoor rainproof plastic greenhouse in Yachiyo-cho, Yuki-gun, Ibaraki Prefecture from June to September 2021 and from May to September 2022. During the test period, the lowest temperature in the plastic greenhouse was 14.7℃, the highest temperature was 41.1℃, and the average temperature was 24.8℃.

(試驗方法) (Test method)

將經上述調製的各試料(產生器)設在防雨塑膠型溫室內後，評價1至90天(或100天)的期間是否有2-己烯醛的氣味及變質。評價係由熟悉2-己烯醛的氣味之3名評價者進行。評價結果如下：將塑膠型溫室內的夾鍊袋中施用的各試料(產生器)帶回實驗室，3名評價者在無風狀態下以距離鼻尖5cm的情形評價氣味之結果呈示平均值(依照下述5階段評價標準的評價值)。 After placing the above-prepared samples (generators) in a rainproof plastic greenhouse, evaluate whether there is 2-hexenal odor and deterioration during 1 to 90 days (or 100 days). The evaluation is conducted by 3 evaluators who are familiar with the odor of 2-hexenal. The evaluation results are as follows: Each sample (generator) applied in the ziplock bag in the plastic greenhouse was brought back to the laboratory, and the 3 evaluators evaluated the odor at a distance of 5 cm from the nose tip in a windless state. The average value is presented (according to the evaluation value of the following 5-stage evaluation standard).

(評價基準) (Evaluation criteria)

4：僅非常強烈的感覺到2-己烯醛之氣味的情形(有氣味) 4: The odor of 2-hexenal is only very strong (there is odor)

3：2-己烯醛的氣味為主要的氣味，但可感覺到有些氣味的變質(因氧化產生的2-己烯酸氣味等)的情形(輕微變質) 3: The smell of 2-hexenal is the main smell, but some deterioration can be felt (such as the smell of 2-hexenoic acid produced by oxidation) (slight deterioration)

2：2-己烯醛氣味仍然存在，但氣味變質(因氧化等產生的2-己烯酸氣味)也很明顯的情形(有些變質) 2: The smell of 2-hexenal still exists, but the smell is also obviously deteriorated (the smell of 2-hexenoic acid produced by oxidation, etc.) (somewhat deteriorated)

1：有氣味，但非2-己烯醛的氣味，而是完全變質之氣味(因氧化產生的2-己烯酸氣味等)的情形(變質) 1: There is an odor, but it is not the odor of 2-hexenal, but the odor of complete deterioration (the odor of 2-hexenoic acid produced by oxidation, etc.) (deterioration)

0：靜置5分鐘也無氣味的情形(無氣味) 0: No odor even after 5 minutes of standing still (no odor)

(試驗結果) (Test results)

將各試料(實施例及比較例)的官能評價結果呈示在表1中。 The sensory evaluation results of each sample (Example and Comparative Example) are presented in Table 1.

[表1]

[Table 1]

依照表1，無論使用那種產生器，藉由在2-己烯醛中添加檸檬酸三乙酯，和單獨添加2-己烯醛或2-己烯醛中添加其他的添加劑(例如抗氧化劑、 紫外線吸收劑、精油成分、PEG400、IP溶劑2835(溶劑)等)的情形相比，確認可使有效成分化合物的2-己烯醛明顯穩定，抑制其變質。此外，確認藉由使用本發明的組成物，可明顯抑制2-己烯醛隨著時間的減少質量，且其緩釋作用可持續2個月以上的長時期。特別可確認藉由使用圖3所述之耐受性增強劑的產生器C(本發明的耐受性增強劑的產生器)，2-己烯醛可在更長期間(3個月以上)幾乎不變質的釋放。 According to Table 1, regardless of the generator used, by adding triethyl citrate to 2-hexenal, it was confirmed that the active ingredient compound 2-hexenal can be significantly stabilized and its deterioration can be suppressed compared with the case where 2-hexenal is added alone or other additives (such as antioxidants, ultraviolet absorbers, essential oil components, PEG400, IP solvent 2835 (solvent) etc.) are added to 2-hexenal. In addition, it was confirmed that by using the composition of the present invention, the quality reduction of 2-hexenal over time can be significantly suppressed, and its sustained release effect can last for a long period of more than 2 months. In particular, it was confirmed that by using the tolerance enhancer generator C (the tolerance enhancer generator of the present invention) shown in FIG. 3 , 2-hexenal can be released for a longer period of time (more than 3 months) without deterioration.

[產業上應用的可能性] [Possibility of industrial application]

依照本發明，可提供一種植物之耐受性增強劑、使用該耐受性增強劑的耐受性增強方法，及簡便且有效產生該耐受性增強劑的器具，其可持續2個月以上增強或改善植物的耐熱性、耐乾燥性等耐受性，且在農業現場中不需頻繁更換，可整個季節(2個月以上的夏季期間)使用。 According to the present invention, a plant tolerance enhancer, a tolerance enhancing method using the tolerance enhancer, and a simple and effective device for producing the tolerance enhancer can be provided. The tolerance enhancer can enhance or improve the heat resistance, drought resistance, etc. of plants for more than 2 months, and does not need to be frequently replaced in agricultural fields, and can be used throughout the season (more than 2 months in summer).

又，實施型態中說明的植物之耐受性增強劑的產生器，係說明本發明的要旨之一例，在不脫離本發明的要旨之範圍中也包含其變形或改造。 In addition, the plant tolerance enhancer generator described in the embodiment is an example to illustrate the gist of the present invention, and its modification or transformation is also included within the scope of the gist of the present invention.

本申請案係將2022年9月27日向日本申請之日本特願2022-153841作為基礎，並將其所有內容包含在本說明書中。 This application is based on Japanese Patent Application No. 2022-153841 filed in Japan on September 27, 2022, and all its contents are included in this specification.

Claims (16)

一種耐受性增強劑，係用以持續2個月以上增強或改善植物的耐熱性及耐乾燥性者，其含有選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物、及檸檬酸三乙酯。 A tolerance enhancer is used to enhance or improve the heat resistance and desiccation resistance of plants for more than 2 months, comprising an unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the end, selected from the group consisting of α , β -enal, α , β -enone, γ , δ -enal, γ , δ -enone , δ , ε -enal, δ , ε-enone, ε , ζ -enal and ε , ζ -enone, and triethyl citrate. 如請求項1所述之耐受性增強劑，其中，末端不具有不飽和鍵之碳數4至9的不飽和羧基化合物係選自由α,β-烯醛、γ,δ-烯醛、δ,ε-烯醛及ε,ζ-烯醛所組成之群組中的至少1種。 The tolerance enhancer as described in claim 1, wherein the unsaturated carboxyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal is at least one selected from the group consisting of α , β -enal, γ , δ -enal, δ , ε -enal and ε , ζ -enal. 如請求項1所述之耐受性增強劑，其中，末端不具有不飽和鍵之碳數4至9的不飽和羧基化合物為α,β-烯醛。 The tolerance enhancer as described in claim 1, wherein the unsaturated carboxyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal is α , β -enal. 一種持續2個月以上增強或改善植物之耐熱性及耐乾燥性的方法，其包括下列步驟： A method for enhancing or improving the heat and drought tolerance of plants for more than 2 months, comprising the following steps: (1)將選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物和檸檬酸三乙酯混合，而調製含有該等之組成物的步驟；及 (1) a step of preparing a composition containing at least one unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal selected from the group consisting of α, β - enal, α , β - enone , γ , δ-enal, γ , δ -enone, δ , ε -enal, δ, ε-enone, ε, ζ-enal and ε , ζ -enone by mixing with triethyl citrate; and (2)將上述末端不具有不飽和鍵的碳數4至9之不飽和羧基化合物釋放至大氣中的步驟。 (2) A step of releasing the unsaturated carboxyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal into the atmosphere. 如請求項4所述之方法，其中，末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物係選自由α,β-烯醛、γ,δ-烯醛、δ,ε-烯醛及ε,ζ-烯醛所組成之群組中的至少1種。 The method as described in claim 4, wherein the unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal is at least one selected from the group consisting of α , β -enal, γ , δ -enal, δ , ε -enal and ε , ζ -enal. 如請求項4所述之方法，其中，末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物為α,β-烯醛。 The method as described in claim 4, wherein the unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal is α , β -enal. 如請求項4至6中任一項所述之方法，係藉由使用耐受性增強劑的產生器，並將前述可撓性容器折彎，使前述易碎性容器破裂而進行前述(1)及(2)的步驟，其中，在可設置一個以上的散發孔之可撓性容器內收納至少一個易碎性容器，該易碎性容器含有選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物，前述可撓性容器中具有散發孔時，將散發孔密封，無散發孔時，在末端配置吸收性材料，且在易碎性容器的外側且可撓性容器內收納含有檸檬酸三乙酯的液劑。 The method as described in any one of claims 4 to 6 is performed by using a generator of a tolerance enhancer and bending the flexible container to break the fragile container to perform the steps (1) and (2) above, wherein at least one fragile container is contained in a flexible container that can be provided with one or more diffusion holes, and the fragile container contains a compound selected from the group consisting of α , β -enal, α , β -enone, γ , δ -enal, γ , δ -enone, δ , ε -enal, δ , ε -enone, ε , ζ -enal and ε , ζ -an unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal of at least one of the groups consisting of alkenone and ketone; when the flexible container has diffusion holes, the diffusion holes are sealed; when there are no diffusion holes, an absorbent material is arranged at the terminal; and a liquid containing triethyl citrate is stored outside the frangible container and inside the flexible container. 如請求項4至6中任一項所述之方法，係藉由使用耐受性增強劑的產生器，並將可撓性容器折彎，使第1易碎性容器及第2易碎性容器破裂而進行前述(1)及(2)的步驟，其中，在可設置一個以上的散發孔之可撓性容器內收納第1易碎性容器，該第1易碎性容器係含有選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物，前述可撓性容器中具有散發孔時，將散發孔密封，無散發孔時，則在末端配置吸收性材料，且在第1易碎性容器的外側且可撓性容器內收納含有檸檬酸三乙酯的第2易碎性容器。 The method as described in any one of claims 4 to 6 is performed by using a generator of a tolerance enhancer and bending a flexible container to break the first and second fragile containers to perform the steps (1) and (2) above, wherein the first fragile container is accommodated in a flexible container that can be provided with one or more diffusion holes, and the first fragile container contains a compound selected from the group consisting of α , β -enal, α , β -enone, γ , δ -enal, γ , δ -enone, δ , ε -enal, δ , ε -enone, ε , ζ -enal and ε , ζ -an unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal of at least one of the groups consisting of alkenone and ketone; when the flexible container has diffusion holes, the diffusion holes are sealed; when there are no diffusion holes, an absorbent material is arranged at the terminal; and a second frangible container containing triethyl citrate is placed outside the first frangible container and inside the flexible container. 如請求項4至6中任一項所述之方法，係使用用以持續2個月以上增強或改善植物的耐熱性及耐乾燥性用之耐受性增強劑的產生器，並將前述可撓性容器折彎，使前述易碎性容器破裂而進行前述(1)及(2)的步驟，其中， 該耐受性增強劑的產生器具備：頂部密封、底部開放的圓筒狀可撓性容器，***於前述可撓性容器內部的收納空間之含有選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物的至少一個易碎性容器，附加在前述可撓性容器的底部之附件，及安裝在前述附件和前述可撓性容器之間的滲透膜，其中，前述附件具有與前述可撓性容器之內面相對向的側面及與前述可撓性容器之底部相對向的上表面，前述附件在前述表面具有貫穿孔，同時在前述側面具有一個或多個空隙，且在前述易碎性容器的外側且可撓性容器內收納含有檸檬酸三乙酯之液劑。 The method as described in any one of claims 4 to 6 is to use a tolerance enhancer generator for enhancing or improving the heat resistance and desiccation resistance of plants for more than 2 months, and to bend the flexible container to break the fragile container to perform the steps (1) and (2), wherein the tolerance enhancer generator comprises: a cylindrical flexible container with a sealed top and an open bottom, and a storage space inserted into the flexible container containing a substance selected from the group consisting of α , β -enal, α , β -enone, γ , δ -enal, γ , δ -enone, δ , ε -enal, δ , ε -enone, ε , ζ -enal and ε , ζ-enone . At least one frangible container containing at least one unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the end of the group consisting of at least one of the group consisting of cyclopentane and cyclopentane, an attachment attached to the bottom of the flexible container, and a permeable membrane installed between the attachment and the flexible container, wherein the attachment has a side surface opposite to the inner surface of the flexible container and an upper surface opposite to the bottom of the flexible container, the attachment has a through hole on the surface and one or more gaps on the side surface, and a liquid containing triethyl citrate is stored outside the frangible container and inside the flexible container. 如請求項4至6中任一項所述之方法，係使用用以持續2個月以上增強或改善植物的耐熱性及耐乾燥性用之耐受性增強劑的產生器，並將前述可撓性容器折彎，使前述易碎性容器破裂而進行前述(1)及(2)的步驟，其中，該耐受性增強劑的產生器具備：頂部密封、底部開放的圓筒狀可撓性容器，***於前述可撓性容器內部的收納空間之含有選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物的第1易碎性容器，附加在前述可撓性容器的底部之附件，及安裝在前述附件和前述可撓性容器之間的滲透膜，其中，前述附件具有與前述可撓性容器之內面相對向的側面及與前述可撓性容器之底部相對向的上表面，前述附件在前述表面具有貫穿孔，同時在前述側面具有一個或多個空隙，且在第1易碎性容器的外側且可撓性容器內收納含有檸檬酸三乙酯之第2易碎性容器。 The method as described in any one of claims 4 to 6 is to use a generator for enhancing or improving the heat resistance and desiccation resistance of plants for more than 2 months, and to bend the flexible container to break the fragile container to perform the steps (1) and (2), wherein the generator for the tolerance enhancer comprises: a cylindrical flexible container with a sealed top and an open bottom, and a storage space inserted into the flexible container containing a substance selected from the group consisting of α , β -enal, α , β -enone, γ , δ -enal, γ , δ -enone, δ , ε -enal, δ , ε -enone, ε , ζ -enal and ε , ζ-enal . -enone, an attachment attached to the bottom of the flexible container, and a permeable membrane installed between the attachment and the flexible container, wherein the attachment has a side surface opposite to the inner surface of the flexible container and an upper surface opposite to the bottom of the flexible container, the attachment has a through hole on the surface and one or more gaps on the side surface, and a second frangible container containing triethyl citrate is accommodated outside the first frangible container and inside the flexible container. 一種耐受性增強劑的產生器，該耐受性增強劑係用以持續2個月以上增強或改善植物的耐熱性及耐乾燥性者，其中，在可設置一個以上的散發孔之可撓性容器內收納至少一個易碎性容器，該易碎性容器係含有選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物，前述可撓性容器中具有散發孔時，將散發孔密封，或無散發孔時，則在末端配置吸收性材料，且在易碎性容器的外側且可撓性容器內收納含有檸檬酸三乙酯的液劑。 A tolerance enhancer generator, the tolerance enhancer is used to enhance or improve the heat resistance and desiccation resistance of plants for more than 2 months, wherein at least one fragile container is contained in a flexible container with more than one diffusion hole, and the fragile container contains a compound selected from α , β -enal, α , β -enone, γ , δ -enal, γ , δ -enone, δ , ε -enal, δ , ε -enone, ε , ζ -enal and ε , ζ-enone . -an unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal of at least one of the groups consisting of alkenones; when the flexible container has diffusion holes, the diffusion holes are sealed; or when there are no diffusion holes, an absorbent material is arranged at the terminal; and a liquid containing triethyl citrate is stored outside the frangible container and inside the flexible container. 一種耐受性增強劑的產生器，該耐受性增強劑係用以持續2個月以上增強或改善植物的耐熱性及耐乾燥性者，其中，在可設置一個以上的散發孔之可撓性容器內，收納第1易碎性容器，該第1易碎性容器係含有選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物，前述可撓性容器中具有散發孔時，將散發孔密封，或無散發孔時，則在末端配置吸收性材料，且在第1易碎性容器的外側且可撓性容器內收納含有檸檬酸三乙酯的第2易碎性容器。 A tolerance enhancer generator, the tolerance enhancer is used to enhance or improve the heat resistance and desiccation resistance of plants for more than 2 months, wherein a first fragile container is stored in a flexible container with more than one diffusion hole, and the first fragile container contains a compound selected from α , β -enal, α , β -enone, γ , δ -enal, γ , δ -enone, δ , ε -enal, δ , ε -enone, ε , ζ -enal and ε , ζ-enal . -an unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal of at least one of the group consisting of alkenone and ketone; when the flexible container has diffusion holes, the diffusion holes are sealed; when there are no diffusion holes, an absorbent material is arranged at the terminal; and a second frangible container containing triethyl citrate is accommodated outside the first frangible container and inside the flexible container. 一種耐受性增強劑的產生器，該耐受性增強劑係用以持續2個月以上增強或改善植物的耐熱性及耐乾燥性者，其中，該耐受性增強劑的產生器具備：頂部密封、底部開放的圓筒狀可撓性容器，***於前述可撓性容器內部的收納空間之含有選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物的至少一個易碎性容器，附加 在前述可撓性容器的底部之附件，及安裝在前述附件與前述可撓性容器之間的滲透膜，前述附件具有與前述可撓性容器的內面相對向之側面及與前述可撓性容器的底部相對向之上表面，前述附件在前述表面具有貫穿孔，同時在前述側面具有一個或多個的空隙，且在前述易碎性容器的外側且可撓性容器內收納含有檸檬酸三乙酯之液劑。 A tolerance enhancer generator, the tolerance enhancer is used to enhance or improve the heat resistance and desiccation resistance of plants for more than 2 months, wherein the tolerance enhancer generator comprises: a cylindrical flexible container with a sealed top and an open bottom, and a storage space inserted into the flexible container containing a compound selected from the group consisting of α , β -enal, α , β -enone, γ , δ -enal, γ , δ -enone, δ , ε -enal, δ , ε -enone, ε , ζ -enal and ε , ζ -enone. At least one frangible container containing at least one unsaturated carbonyl compound having 4 to 9 carbon atoms and having no unsaturated bond at the terminal of the group consisting of at least one of olefins and ketones, an attachment attached to the bottom of the above-mentioned flexible container, and a permeable membrane installed between the above-mentioned attachment and the above-mentioned flexible container, wherein the above-mentioned attachment has a side surface opposite to the inner surface of the above-mentioned flexible container and an upper surface opposite to the bottom of the above-mentioned flexible container, the above-mentioned attachment has a through hole on the above-mentioned surface, and at the same time has one or more gaps on the above-mentioned side surface, and a liquid containing triethyl citrate is stored on the outer side of the above-mentioned frangible container and in the flexible container. 一種耐受性增強劑的產生器，該耐受性增強劑係用以持續2個月以上增強或改善植物的耐熱性及耐乾燥性者，其中，該耐受性增強劑的產生器具備：頂部密封、底部開放的圓筒狀可撓性容器，***於前述可撓性容器內部的收納空間之含有選自由α,β-烯醛、α,β-烯酮、γ,δ-烯醛、γ,δ-烯酮、δ,ε-烯醛、δ,ε-烯酮、ε,ζ-烯醛及ε,ζ-烯酮所組成之群組中的至少1種之末端不具有不飽和鍵的碳數4至9之不飽和羰基化合物的第1易碎性容器，附加在前述可撓性容器的底部之附件，及安裝在前述附件與前述可撓性容器之間的滲透膜，前述附件具有瓦前述可撓性容器的內面相對向之側面及與前述可撓性容器的底部相對向之上表面，前述附件在前述表面具有貫穿孔，同時在前述側面具有一個或多個的空隙，且在第1易碎性容器的外側且可撓性容器內收納含有檸檬酸三乙酯之第2易碎性容器。 A tolerance enhancer generator, the tolerance enhancer is used to enhance or improve the heat resistance and desiccation resistance of plants for more than 2 months, wherein the tolerance enhancer generator comprises: a cylindrical flexible container with a sealed top and an open bottom, and a storage space inserted into the flexible container containing a compound selected from the group consisting of α , β -enal, α , β -enone, γ , δ -enal, γ , δ -enone, δ , ε -enal, δ , ε -enone, ε , ζ -enal and ε , ζ -enone. A first frangible container containing at least one unsaturated carbonyl compound with 4 to 9 carbon atoms and no unsaturated bond at the terminal of the compound, an attachment attached to the bottom of the flexible container, and a permeable membrane installed between the attachment and the flexible container, wherein the attachment has a side surface opposite to the inner surface of the flexible container and an upper surface opposite to the bottom of the flexible container, the attachment has a through hole on the surface and one or more gaps on the side surface, and a second frangible container containing triethyl citrate is accommodated outside the first frangible container and inside the flexible container. 如請求項13或14所述之耐受性增強劑的產生器，其中，前述可撓性容器和前述滲透膜之間更具備至少具有一個開口部的支撐具。 The tolerance enhancer generator as described in claim 13 or 14, wherein a support having at least one opening is provided between the flexible container and the permeable membrane. 如請求項11至15中任一項所述之耐受性增強劑的產生器，其中，可撓性容器係選自由聚丙烯、聚乙烯、聚胺酯、聚醯胺及聚氯乙烯所組成之群組中的樹脂製造，且易碎性容器為玻璃安瓿。 A generator of a tolerance enhancer as described in any one of claims 11 to 15, wherein the flexible container is made of a resin selected from the group consisting of polypropylene, polyethylene, polyurethane, polyamide and polyvinyl chloride, and the fragile container is a glass ampoule.