TW202326109A

TW202326109A - Convective polymerase chain reaction apparatus and optical detecting method thereof

Info

Publication number: TW202326109A
Application number: TW110149352A
Authority: TW
Inventors: 林俊全; 曹孝岳; 羅時斌; 溫國興; 郭建志; 巫秉融; 洪瑞賢; 陳庭軒
Original assignee: 財團法人工業技術研究院
Priority date: 2021-12-29
Filing date: 2021-12-29
Publication date: 2023-07-01
Also published as: TWI818398B

Abstract

A convective polymerase chain reaction apparatus and an optical detecting method thereof are provided. The optical detecting method includes the following steps. The substance in the tube to be tested is heated. At least two monochromatic lights are provided and combined by a light combining element and then to irradiate the tube to be tested. At least two kinds of excited lights generated by the excitation of the substance in the tube to be tested by the at least two monochromatic lights are sensed.

Description

熱對流聚合酶連鎖反應裝置及其光學偵測方法Thermal convection polymerase chain reaction device and optical detection method thereof

本揭露是有關於一種反應裝置及偵測方法，且特別是有關於一種熱對流聚合酶連鎖反應裝置及其光學偵測方法。The present disclosure relates to a reaction device and a detection method, and in particular to a thermal convection polymerase chain reaction device and an optical detection method thereof.

隨著各種傳染病的盛行，亟需快速且精準的病毒檢測方法以預防與監控傳染病大流行。利用核酸萃取與聚合酶連鎖反應而進行的病毒核酸檢測可以偵測受測者的體內是否帶有病毒的基因片段。這個檢測方法的靈敏度高，只要受測者的體內有少量病毒即可測出。With the prevalence of various infectious diseases, rapid and accurate virus detection methods are urgently needed to prevent and monitor the pandemic of infectious diseases. Viral nucleic acid detection using nucleic acid extraction and polymerase chain reaction can detect whether the subject has a gene fragment of the virus in the body. The sensitivity of this detection method is high, as long as there is a small amount of virus in the subject's body, it can be detected.

在熱對流聚合酶連鎖反應中，採用熱對流原理對盛裝待測液體的反應容器直接加熱，使待測液體產生溫度梯度並造成熱對流現象。藉由使待測液體在熱對流的過程中不斷改變溫度，待測液體的熱對流可使核酸擴增，縮短傳統方法變換溫度所需的時間，並且具有測試裝置的結構簡單與成本低廉的優點。目前所採用的熱對流聚合酶連鎖反應裝置，是以塑膠或玻璃等材質的毛細試管作為反應容器，並以光線照射試管以激發管內的螢光試劑產生螢光訊號，再藉由感測器感測螢光訊號而達到監控聚合酶連鎖反應的目的。In the thermal convection polymerase chain reaction, the thermal convection principle is used to directly heat the reaction container containing the liquid to be tested, so that the liquid to be tested produces a temperature gradient and causes heat convection. By making the temperature of the liquid to be tested constantly change during the process of heat convection, the heat convection of the liquid to be tested can amplify nucleic acid, shorten the time required for the traditional method to change the temperature, and has the advantages of simple structure and low cost of the test device . The thermal convection polymerase chain reaction device currently used uses a capillary test tube made of plastic or glass as a reaction vessel, and irradiates the test tube with light to excite the fluorescent reagent in the tube to generate a fluorescent signal, and then through the sensor The purpose of monitoring the polymerase chain reaction is achieved by sensing the fluorescent signal.

聚合酶連鎖反應是一種分子生物學技術，用於擴增特定的去氧核醣核酸(Deoxyribonucleic Acid，DNA)或核糖核酸(Ribonucleic acid，RNA)片段。典型的聚合酶連鎖反應裝置是採用熱循環機(thermocycler)的反覆加熱和冷卻來重複進行熱循環步驟。經過金屬塊將熱能逐漸傳導(thermal conduction)至反應試管內，以使去氧核醣核酸或核醣核酸片段在不同溫度下產生變性(95ºC)、黏合(45ºC-65ºC)及延伸(72ºC)等三種不同的溫度循環。然而，當要檢測的目標病毒的種類不只一種時，每一個裝置只能對一個待測試管檢測一種病毒，需根據目標病毒的種類數量而對應採檢取得相同數量的待測試管，增加受測者的負擔。Polymerase chain reaction is a molecular biology technique for amplifying specific deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) fragments. A typical polymerase chain reaction device uses a thermocycler to repeatedly heat and cool to repeat the thermocycling steps. The heat energy is gradually conducted (thermal conduction) into the reaction test tube through the metal block, so that DNA or ribonucleic acid fragments can be denatured (95ºC), bonded (45ºC-65ºC) and extended (72ºC) at different temperatures. temperature cycle. However, when there are more than one type of target virus to be detected, each device can only detect one type of virus for one test tube, and it is necessary to obtain the same number of test tubes according to the number of target virus types to increase the number of test tubes to be tested. the burden of the recipient.

本揭露提供一種熱對流聚合酶連鎖反應裝置及其光學偵測方法，可同時檢測多種目標物，進而減少整體所需的檢體的量。The disclosure provides a thermal convection polymerase chain reaction device and an optical detection method thereof, which can simultaneously detect multiple targets, thereby reducing the overall required sample amount.

本揭露的熱對流聚合酶連鎖反應裝置包括一座體、一加熱元件、一合光元件、至少二發光元件以及至少二光感測器。光感測器的數量對應於發光元件的數量。座體適於承載一待測試管。加熱元件安裝於座體，以透過座體加熱待測試管。合光元件包括相連的一第一板狀部、一第二板狀部、一第三板狀部與一第四板狀部。第一板狀部與第二板狀部界定一出光區。第二板狀部與第三板狀部界定一第一入光區。第三板狀部與第四板狀部界定一第二入光區。第一板狀部與第四板狀部界定一第三入光區。發光元件為一第一發光元件、一第二發光元件與一第三發光元件的至少其中兩個。第一發光元件適於朝第一入光區提供一第一色光。第二發光元件適於朝第二入光區提供一第二色光。第三發光元件適於朝第三入光區提供一第三色光。第一板狀部適於反射第一色光並允許第二色光與第三色光通過。第二板狀部適於反射第三色光並允許第一色光與第二色光通過。第三板狀部適於反射第一色光並允許第二色光通過。第四板狀部適於反射第三色光並允許第二色光通過。第一色光、第二色光與第三色光的至少其中兩個進入出光區並朝向座體出光以照射待測試管。第一光感測器、第二光感測器與第三光感測器的至少其中兩個安裝於座體。光感測器安裝於座體，為一第一光感測器、一第二光感測器與一第三光感測器的至少其中兩個。第一光感測器適於接收待測試管內的物質受第一色光激發而產生的一第一激發光。第二光感測器適於接收待測試管內的物質受第二色光激發而產生的一第二激發光。第三光感測器適於接收待測試管內的物質受第三色光激發而產生的一第三激發光。The thermal convection polymerase chain reaction device of the present disclosure includes a base, a heating element, a light-combining element, at least two light-emitting elements and at least two light sensors. The number of photosensors corresponds to the number of light emitting elements. The seat body is suitable for carrying a tube to be tested. The heating element is installed on the base to heat the tube to be tested through the base. The light-combining element includes a first plate-shaped portion, a second plate-shaped portion, a third plate-shaped portion and a fourth plate-shaped portion connected together. The first plate portion and the second plate portion define a light emitting area. The second plate portion and the third plate portion define a first light incident area. The third plate portion and the fourth plate portion define a second light incident area. The first plate portion and the fourth plate portion define a third light incident area. The light emitting element is at least two of a first light emitting element, a second light emitting element and a third light emitting element. The first light emitting element is adapted to provide a first color light toward the first light incident area. The second light emitting element is adapted to provide a second color light toward the second light incident area. The third light emitting element is adapted to provide a third color light toward the third light incident area. The first plate portion is suitable for reflecting the first color light and allowing the second color light and the third color light to pass through. The second plate portion is suitable for reflecting the third color light and allowing the first color light and the second color light to pass through. The third plate portion is adapted to reflect the first color light and allow the second color light to pass through. The fourth plate portion is adapted to reflect the third color light and allow the second color light to pass through. At least two of the first color light, the second color light and the third color light enter the light exit area and emit light toward the base to irradiate the tube to be tested. At least two of the first light sensor, the second light sensor and the third light sensor are installed on the base. The light sensor is installed on the base body and is at least two of a first light sensor, a second light sensor and a third light sensor. The first light sensor is suitable for receiving a first excitation light generated by the substance in the tube to be tested excited by the first color light. The second light sensor is suitable for receiving a second excitation light generated by the substance in the tube to be tested excited by the second color light. The third light sensor is suitable for receiving a third excitation light generated by the substance in the tube to be tested excited by the third color light.

本揭露的光學偵測方法使用上述的熱對流聚合酶連鎖反應裝置進行檢測，其方法包括下列步驟，加熱待測試管內的物質。提供第一色光、第二色光與第三色光的至少其中兩個並使用合光元件進行合光，合光後照射待測試管。感測待測試管內的物質受第一色光激發而產生的第一激發光、受第二色光激發而產生的第二激發光以及受第三色光激發而產生的第三激發光的至少其中兩個。The optical detection method of the present disclosure uses the thermal convection polymerase chain reaction device for detection, and the method includes the following steps of heating the substance in the test tube. At least two of the first color light, the second color light and the third color light are provided and the light combination element is used to combine the light, and after the light combination, the tube to be tested is irradiated. Sensing at least one of the first excitation light generated by the excitation of the first color light, the second excitation light generated by the second color light excitation and the third excitation light generated by the third color light excitation of the substance in the tube to be tested two.

基於上述，在本揭露的熱對流聚合酶連鎖反應裝置及其光學偵測方法中，可以至少兩種色光同時進行檢測，進而減少檢體的用量。Based on the above, in the thermal convection polymerase chain reaction device and the optical detection method thereof of the present disclosure, at least two kinds of color lights can be detected simultaneously, thereby reducing the amount of samples used.

圖1是依照本揭露的一實施例的一種熱對流聚合酶連鎖反應裝置的立體剖視圖。圖2是圖1的熱對流聚合酶連鎖反應裝置的局部上視示意圖，且圖2中省略了熱對流聚合酶連鎖反應裝置的部分元件。請參照圖1與圖2，本實施例的熱對流聚合酶連鎖反應裝置100適於進行熱對流聚合酶連鎖反應，可擴增特定的去氧核醣核酸或核糖核酸片段，以便進行病毒檢測，但本揭露的熱對流聚合酶連鎖反應裝置100並不侷限期使用目的。FIG. 1 is a perspective cross-sectional view of a thermal convection polymerase chain reaction device according to an embodiment of the present disclosure. FIG. 2 is a schematic partial top view of the thermal convective polymerase chain reaction device of FIG. 1 , and some components of the thermal convective polymerase chain reaction device are omitted in FIG. 2 . Please refer to FIG. 1 and FIG. 2 , the thermal convection polymerase chain reaction device 100 of this embodiment is suitable for thermal convection polymerase chain reaction, and can amplify specific DNA or ribonucleic acid fragments for virus detection, but The thermal convection polymerase chain reaction apparatus 100 of the present disclosure is not limited to the purpose of use.

熱對流聚合酶連鎖反應裝置100包括一座體110、一加熱元件130、一合光元件140、至少二發光元件以及至少二光感測器。光感測器的數量是對應發光元件的數量設置。如圖1所示，發光元件可為一第一發光元件150B、一第二發光元件150G與一第三發光元件150R的至少其中兩個；光感測器可為一第一光感測器160B、一第二光感測器160G與一第三光感測器160R的至少其中兩個。換言之，本實施例的熱對流聚合酶連鎖反應裝置100中的發光元件以及光感測器可只包括第一發光元件150B、第二發光元件150G、第一光感測器160B與第二光感測器160G，或只包括第三發光元件150R、第二發光元件150G、第三光感測器160R與第二光感測器160G，或只包括第一發光元件150B、第三發光元件150R、第一光感測器160B與第三光感測器160R，又或同時包括第一發光元件150B、第二發光元件150G、第三發光元件150R、第一光感測器160B、第二光感測器160G與第三光感測器160R。以下，將以熱對流聚合酶連鎖反應裝置100同時包括第一發光元件150B、第二發光元件150G、第三發光元件150R、第一光感測器160B、第二光感測器160G與第三光感測器160R為例做說明。The thermal convection PCR device 100 includes a base 110 , a heating element 130 , a light combining element 140 , at least two light emitting elements and at least two light sensors. The number of light sensors is set corresponding to the number of light emitting elements. As shown in FIG. 1 , the light emitting element can be at least two of a first light emitting element 150B, a second light emitting element 150G and a third light emitting element 150R; the light sensor can be a first light sensor 160B . At least two of a second light sensor 160G and a third light sensor 160R. In other words, the light emitting elements and light sensors in the thermal convection polymerase chain reaction apparatus 100 of this embodiment may only include the first light emitting element 150B, the second light emitting element 150G, the first light sensor 160B and the second light sensor detector 160G, or only include the third light emitting element 150R, the second light emitting element 150G, the third light sensor 160R and the second light sensor 160G, or only include the first light emitting element 150B, the third light emitting element 150R, The first light sensor 160B and the third light sensor 160R, or simultaneously include the first light emitting element 150B, the second light emitting element 150G, the third light emitting element 150R, the first light sensor 160B, the second light sensor detector 160G and the third light sensor 160R. Hereinafter, the thermal convection polymerase chain reaction apparatus 100 will include the first light emitting element 150B, the second light emitting element 150G, the third light emitting element 150R, the first light sensor 160B, the second light sensor 160G and the third light sensor. The light sensor 160R is taken as an example for illustration.

座體110適於承載一待測試管50。加熱元件130安裝於座體110，以透過座體110加熱待測試管50。合光元件140包括相連的一第一板狀部140A、一第二板狀部140B、一第三板狀部140C與一第四板狀部140D。詳細來說，第一板狀部140A、第二板狀部140B、第三板狀部140C與第四板狀部140D各以一表面與其他三者相連，以排列形成X型結構。第一板狀部140A與第二板狀部140B界定一出光區R12。第二板狀部140B與第三板狀部140C界定一第一入光區R14。第三板狀部140C與第四板狀部140D界定一第二入光區R16。第一板狀部140A與第四板狀部140D界定一第三入光區R18。The seat 110 is suitable for carrying a tube 50 to be tested. The heating element 130 is installed on the base body 110 to heat the tube 50 to be tested through the base body 110 . The light combining element 140 includes a first plate-shaped portion 140A, a second plate-shaped portion 140B, a third plate-shaped portion 140C and a fourth plate-shaped portion 140D connected together. In detail, each of the first plate-shaped portion 140A, the second plate-shaped portion 140B, the third plate-shaped portion 140C and the fourth plate-shaped portion 140D is connected to the other three on one surface, and arranged to form an X-shaped structure. The first plate portion 140A and the second plate portion 140B define a light emitting region R12 . The second plate portion 140B and the third plate portion 140C define a first light incident region R14 . The third plate portion 140C and the fourth plate portion 140D define a second light incident region R16 . The first plate portion 140A and the fourth plate portion 140D define a third light incident region R18 .

第一發光元件150B適於朝第一入光區R14提供一第一色光LB。第二發光元件150G適於朝第二入光區R16提供一第二色光LG。第三發光元件150R適於朝第三入光區R18提供一第三色光LR。第一板狀部140A適於反射第一色光LB並允許第二色光LG與第三色光LR通過。第二板狀部140B適於反射第三色光LR並允許第一色光LB與第二色光LG通過。第三板狀部140C適於反射第一色光LB並允許第二色光LG通過。第四板狀部140D適於反射第三色光LR並允許第二色光LG通過。因此，第一板狀部140A與第三板狀部140C可為具有同一種光學特性的分色鏡，第二板狀部140B、第四板狀部140D可同為具有另一種光學特性的分色鏡。第一色光LB、第二色光LG與第三色光LR的至少其中兩個由對應的第一入光區R14、第二入光區R16與第三入光區R18的至少其中兩個進入到合光元件140，並透過第一板狀部140A、第二板狀部140B、第三板狀部140C與第四板狀部140D的光學作用（如反射、穿透）進入至出光區R12，並朝向座體110出光以照射待測試管50。The first light emitting element 150B is adapted to provide a first color light LB toward the first light incident region R14. The second light emitting element 150G is adapted to provide a second color light LG towards the second light incident region R16. The third light emitting element 150R is adapted to provide a third color light LR toward the third light incident region R18. The first plate portion 140A is suitable for reflecting the first color light LB and allowing the second color light LG and the third color light LR to pass through. The second plate portion 140B is adapted to reflect the third color light LR and allow the first color light LB and the second color light LG to pass through. The third plate portion 140C is adapted to reflect the first color light LB and allow the second color light LG to pass through. The fourth plate portion 140D is adapted to reflect the third color light LR and allow the second color light LG to pass through. Therefore, the first plate-shaped part 140A and the third plate-shaped part 140C can be dichroic mirrors with the same optical characteristic, and the second plate-shaped part 140B and the fourth plate-shaped part 140D can both be dichroic mirrors with another optical characteristic. color mirror. At least two of the first color light LB, the second color light LG, and the third color light LR enter from at least two of the corresponding first light entrance region R14, second light entrance region R16, and third light entrance region R18. The light-combining element 140 enters the light exit region R12 through the optical effects (such as reflection and penetration) of the first plate-shaped part 140A, the second plate-shaped part 140B, the third plate-shaped part 140C and the fourth plate-shaped part 140D, And emit light toward the base body 110 to irradiate the tube 50 to be tested.

基於合光元件140的X型結構設計，第一色光LB進入第一入光區R14後可直接穿過第二板狀部140B到達第一板狀部140A，被第一板狀部140A反射後而進入出光區R12，也可被第三板狀部140C反射後再穿過第二板狀部140B而進入出光區R12。第二色光LG進入第二入光區R16後可直接穿過第三板狀部140C與第二板狀部140B而進入出光區R12，也可直接穿過第四板狀部140D與第一板狀部140A而進入出光區R12。第三色光LR進入第三入光區R18後可直接穿過第一板狀部140A到達第二板狀部140B，被第二板狀部140B反射後而進入出光區R12，也可被第四板狀部140D反射後再穿過第一板狀部140A而進入出光區R12。因此，進入不同入光區的第一色光LB、第二色光LG與第三色光LR都會進入出光區R12，並朝向座體110出光以照射待測試管50。Based on the X-shaped structure design of the light combining element 140, the first color light LB enters the first light incident region R14 and can directly pass through the second plate-shaped part 140B to reach the first plate-shaped part 140A, and is reflected by the first plate-shaped part 140A Then enter the light exit region R12, or be reflected by the third plate-shaped portion 140C and then pass through the second plate-shaped portion 140B to enter the light exit region R12. After the second color light LG enters the second light incident region R16, it can directly pass through the third plate portion 140C and the second plate portion 140B to enter the light exit region R12, or directly pass through the fourth plate portion 140D and the first plate. shape portion 140A and enter the light exit region R12. After the third color light LR enters the third light incident region R18, it can directly pass through the first plate-shaped part 140A to reach the second plate-shaped part 140B, be reflected by the second plate-shaped part 140B and then enter the light-emitting region R12, and can also be transmitted by the fourth plate-shaped part 140B. After being reflected by the plate portion 140D, it passes through the first plate portion 140A and enters the light exit region R12. Therefore, the first colored light LB, the second colored light LG and the third colored light LR entering different light incident regions all enter the light emitting region R12 and emit light toward the base 110 to illuminate the tube 50 to be tested.

第一光感測器160B、第二光感測器160G與第三光感測器160R的至少其中兩個安裝於座體110。第一光感測器160B適於接收待測試管50內的物質受第一色光LB激發而產生的一第一激發光EB。第二光感測器160G適於接收待測試管50內的物質受第二色光LG激發而產生的一第二激發光EG。第三光感測器160R適於接收待測試管50內的物質受第三色光LR激發而產生的一第三激發光ER。At least two of the first light sensor 160B, the second light sensor 160G and the third light sensor 160R are mounted on the base body 110 . The first light sensor 160B is suitable for receiving a first excitation light EB generated by the excitation of the substance in the tube 50 to be tested by the first color light LB. The second light sensor 160G is suitable for receiving a second excitation light EG generated by the substance in the test tube 50 excited by the second color light LG. The third light sensor 160R is adapted to receive a third excitation light ER generated by the substance in the tube 50 to be tested excited by the third color light LR.

本實施例的光學偵測方法可使用上述的熱對流聚合酶連鎖反應裝置100進行檢測，其方法包括下列步驟。加熱待測試管50內的物質，使待測試管50內的物質產生溫度梯度並造成熱對流現象，進而產生核酸擴增的現象。此外，提供第一色光LB、第二色光LG與第三色光LR的至少其中兩個，並且使用合光元件140進行合光後照射待測試管50。然後，感測待測試管50內的物質受第一色光LB激發而產生的第一激發光EB、受第二色光LG激發而產生的第二激發光EG以及受第三色光LR激發而產生的第三激發光ER的至少其中兩個。The optical detection method of this embodiment can be detected by using the thermal convection polymerase chain reaction device 100 mentioned above, and the method includes the following steps. Heating the substance in the tube to be tested 50 causes a temperature gradient to be generated in the substance in the tube to be tested 50 and causes thermal convection, thereby generating nucleic acid amplification. In addition, at least two of the first color light LB, the second color light LG, and the third color light LR are provided, and combined by the light combining element 140 to irradiate the tube 50 to be tested. Then, the first excitation light EB generated by the excitation of the first color light LB, the second excitation light EG generated by the excitation of the second color light LG, and the third color light LR generated by the excitation of the substance in the tube 50 to be tested are sensed. At least two of the third excitation light ER.

在本實施例的熱對流聚合酶連鎖反應裝置100中，因為採用了合光元件140而可同時使多種色光同時照射待測試管50，進而讓待測試管50內的不同物質受不同色光激發而產生不同的激發光。由此，可同時檢測待測試管50內的多種物質，進而減少採檢次數，降低受測者的負擔。In the thermal convection polymerase chain reaction device 100 of this embodiment, because the light-combining element 140 is used, multiple colored lights can be simultaneously irradiated on the tubes 50 to be tested, so that different substances in the tubes 50 to be tested can be excited by different colored lights. Different excitation light is generated. In this way, multiple substances in the tube 50 to be tested can be detected simultaneously, thereby reducing the number of inspections and reducing the burden on the subject.

此外，本實施例的合光元件140是由板狀的第一板狀部140A、第二板狀部140B、第三板狀部140C與第四板狀部140D所構成的X型結構，相較於實心立方體式的合光元件具有更輕的重量，也可減少全反射所帶來的問題。另外，因為合光元件140與座體110分離，所以合光元件140旁的第一發光元件150B、第二發光元件150G與第三發光元件150R也會遠離座體110旁的加熱元件130，如此可避免第一發光元件150B、第二發光元件150G與第三發光元件150R受熱而改變發光特性。In addition, the light combining element 140 of this embodiment is an X-shaped structure composed of a plate-shaped first plate-shaped portion 140A, a second plate-shaped portion 140B, a third plate-shaped portion 140C, and a fourth plate-shaped portion 140D. Compared with the solid cube light-combining element, it has lighter weight and can also reduce the problems caused by total reflection. In addition, because the light-combining element 140 is separated from the base body 110, the first light-emitting element 150B, the second light-emitting element 150G, and the third light-emitting element 150R next to the light-combining element 140 are also far away from the heating element 130 next to the base body 110, so It can prevent the first light-emitting element 150B, the second light-emitting element 150G, and the third light-emitting element 150R from being heated to change light-emitting characteristics.

本實施例的座體110適於在其中央承載待測試管50。詳細來說，待測試管50以垂直與水平面之方向設置於座體110中，而第一光感測器160B、第二光感測器160G與第三光感測器160R分別設置在座體110的三個面，加熱元件130則位於座體110的另一個面。在本實施例中，座體110設置第一光感測器160B、第二光感測器160G、第三光感測器160R以及加熱元件130的這四個面，是圍繞於待測試管50。換句話說，第一光感測器160B、第二光感測器160G、第三光感測器160R以及加熱元件13藉由座體110而設置在待測試管50的周圍。第一光感測器160B、第二光感測器160G與第三光感測器160R分別接收第一激發光EB、第二激發光EG與第三激發光ER後，將訊號傳遞至光譜儀60進行分析，以確認待測試管50內是否有設定為檢測目標的物質。The seat body 110 of this embodiment is suitable for carrying the tube 50 to be tested at its center. In detail, the tube 50 to be tested is arranged in the base body 110 in the direction of vertical and horizontal planes, and the first light sensor 160B, the second light sensor 160G and the third light sensor 160R are respectively set in the base body 110 The heating element 130 is located on the other surface of the seat body 110 . In this embodiment, the base body 110 is provided with the first light sensor 160B, the second light sensor 160G, the third light sensor 160R and the four surfaces of the heating element 130, which surround the tube 50 to be tested. . In other words, the first light sensor 160B, the second light sensor 160G, the third light sensor 160R and the heating element 13 are arranged around the tube 50 to be tested through the base 110 . The first light sensor 160B, the second light sensor 160G and the third light sensor 160R respectively receive the first excitation light EB, the second excitation light EG and the third excitation light ER, and transmit the signals to the spectrometer 60 Analysis is performed to confirm whether there is a substance set as a detection target in the tube 50 to be tested.

在本實施例中，熱對流聚合酶連鎖反應裝置100更包括至少二濾光元件。濾光元件可為一第一濾光元件162B、一第二濾光元件162G與一第三濾光元件162R的至少其中兩個。濾光元件的數量是對應光感測器的數量設置，例如是，熱對流聚合酶連鎖反應裝置100包含有第一光感測器160B，則可對應設置有第一濾光元件162B，如熱對流聚合酶連鎖反應裝置100包含有第二光感測器160G，則可對應設置有第二濾光元件162G，如熱對流聚合酶連鎖反應裝置100包含有第三光感測器160R，則可對應設置有第三濾光元件162R。詳細來說，第一濾光元件162B設置於第一光感測器160B與座體110之間。第二濾光元件162G設置於第二光感測器160G與座體110之間。第三濾光元件162R設置於第三光感測器160R與座體110之間。濾光元件可先對激發光進行濾光後再感測，例如是，第一濾光元件162B可以進一步濾除第一激發光EB以外的光線，以避免第一光感測器160B接收到第二激發光EG及第三激發光ER而降低感測的精確度。第二濾光元件162G可以進一步濾除第二激發光EG以外的光線，以避免第二光感測器160G接收到第一激發光EB以及第三激發光ER而降低感測的精確度。第三濾光元件162R可以進一步濾除第三激發光ER以外的光線，以避免第三光感測器160R接收到第一激發光EB及第二激發光EG而降低感測的精確度。In this embodiment, the thermal convection PCR device 100 further includes at least two filter elements. The filter element can be at least two of a first filter element 162B, a second filter element 162G, and a third filter element 162R. The number of filter elements is set corresponding to the number of photosensors. For example, if the thermal convection polymerase chain reaction device 100 includes a first photosensor 160B, the first filter element 162B can be correspondingly provided, such as a thermal convection polymerase chain reaction device 100. The convection polymerase chain reaction device 100 includes the second light sensor 160G, and the second filter element 162G can be correspondingly provided. If the thermal convection polymerase chain reaction device 100 includes the third light sensor 160R, then the Correspondingly, a third filter element 162R is provided. In detail, the first filter element 162B is disposed between the first light sensor 160B and the base 110 . The second filter element 162G is disposed between the second light sensor 160G and the base 110 . The third filter element 162R is disposed between the third light sensor 160R and the base 110 . The filter element can first filter the excitation light before sensing, for example, the first filter element 162B can further filter out light other than the first excitation light EB, so as to prevent the first light sensor 160B from receiving the first light The second excitation light EG and the third excitation light ER reduce the sensing accuracy. The second filter element 162G can further filter out light other than the second excitation light EG, so as to prevent the second light sensor 160G from receiving the first excitation light EB and the third excitation light ER to reduce the sensing accuracy. The third filter element 162R can further filter out the light other than the third excitation light ER, so as to prevent the third light sensor 160R from receiving the first excitation light EB and the second excitation light EG to reduce the sensing accuracy.

本實施例的熱對流聚合酶連鎖反應裝置100還可包括一電路板170。座體110與加熱元件130可設置在電路板170上，而合光元件140可設置在電路板170的下方。第一發光元件150B、第二發光元件150G與第三發光元件150R可電性連接至電路板170。The thermal convection polymerase chain reaction device 100 of this embodiment may further include a circuit board 170 . The base body 110 and the heating element 130 can be disposed on the circuit board 170 , and the light combining element 140 can be disposed under the circuit board 170 . The first light emitting element 150B, the second light emitting element 150G and the third light emitting element 150R are electrically connected to the circuit board 170 .

在本實施例中，座體110具有多個細長通孔112。細長通孔112的長軸A10垂直於承載在座體110內的待測試管50的長軸A20。多個細長通孔112可對應分別連通於第一光感測器160B、第二光感測器160G及第三光感測器160R，以供第一激發光EB、第二激發光EG及第三激發光ER通過。細長通孔112可對第一激發光EB、第二激發光EG以及第三激發光ER產生准直的效果。細長通孔112垂直於待測試管50，也就相當於垂直於三種色光照射待測試管50時的入光方向。因此，可以大幅降低三種色光通過細長通孔112而入射三種感測器的機率，進而降低干擾並提高感測的精確度。此外，為了提高進光量，可以對於每個感測器設置多個對應的細長通孔112，使激發光通過細長通孔112進行准直後再感測。如圖1所示，在本實施例中，沿著承載在座體110內的待測試管50的長軸A20排列的三個細長通孔112對應連通於第三感測器160R，以供第一激發光EB、第二激發光EG及第三激發光ER通過，並由第三濾光元件162R濾除雜訊後傳至第三感測器160R。In this embodiment, the base body 110 has a plurality of elongated through holes 112 . The long axis A10 of the elongated through hole 112 is perpendicular to the long axis A20 of the tube 50 to be tested carried in the seat 110 . A plurality of elongated through holes 112 can be respectively communicated with the first light sensor 160B, the second light sensor 160G and the third light sensor 160R for the first excitation light EB, the second excitation light EG and the third light sensor 160R. Three excitation light ER passes through. The elongated through hole 112 can collimate the first excitation light EB, the second excitation light EG and the third excitation light ER. The elongated through hole 112 is perpendicular to the tube 50 to be tested, which is equivalent to being perpendicular to the light incident directions when the three colored lights irradiate the tube 50 to be tested. Therefore, the probability of the three kinds of colored lights entering the three sensors through the elongated through hole 112 can be greatly reduced, thereby reducing interference and improving sensing accuracy. In addition, in order to increase the amount of incoming light, a plurality of corresponding elongated through holes 112 may be provided for each sensor, so that the excitation light passes through the elongated through holes 112 to be collimated and then sensed. As shown in FIG. 1 , in this embodiment, three elongated through holes 112 arranged along the long axis A20 of the tube to be tested 50 carried in the seat 110 correspond to communicate with the third sensor 160R for the first The excitation light EB, the second excitation light EG and the third excitation light ER pass through, and are transmitted to the third sensor 160R after being filtered by the third filter element 162R.

在本實施例中，每一發光元件可包括一發光二極體與一濾光器。詳細來說，第一發光元件150B包括一發光二極體150B1與一濾光器150B2。濾光器150B2設置於發光二極體150B1與合光元件140之間。濾光器150B2可確保照射至待測試管50的第一色光LB具有預設的波長，以提高感測的精確度。類似地，第二發光元件150G包括一發光二極體150G1與一濾光器150G2。濾光器150G2設置於發光二極體150G1與合光元件140之間。第三發光元件150R包括一發光二極體150R1與一濾光器150R2。濾光器150R2設置於發光二極體150R1與合光元件140之間。發光二極體可以是有機或無機的發光二極體。In this embodiment, each light emitting element may include a light emitting diode and a filter. In detail, the first light emitting element 150B includes a light emitting diode 150B1 and a filter 150B2. The filter 150B2 is disposed between the light-emitting diode 150B1 and the light-combining element 140 . The filter 150B2 can ensure that the first color light LB irradiated to the tube 50 to be tested has a predetermined wavelength, so as to improve the accuracy of sensing. Similarly, the second light emitting element 150G includes a light emitting diode 150G1 and a filter 150G2 . The filter 150G2 is disposed between the light-emitting diode 150G1 and the light-combining element 140 . The third light emitting element 150R includes a light emitting diode 150R1 and a filter 150R2 . The filter 150R2 is disposed between the light-emitting diode 150R1 and the light-combining element 140 . The light emitting diodes may be organic or inorganic light emitting diodes.

圖3是說明圖1的熱對流聚合酶連鎖反應裝置的聚光單元的作用的示意圖。請參照圖3，在本實施例中，熱對流聚合酶連鎖反應裝置100更包括一聚光單元180，設置於合光元件140的出光區R12與座體110之間，適於將第一色光LB、第二色光LG與第三色光LR的至少其中兩個聚焦在待測試管50上，且適於調整聚焦的位置。圖4是圖3的熱對流聚合酶連鎖反應裝置的聚光單元經調整後的示意圖，且圖3與圖4中省略了熱對流聚合酶連鎖反應裝置的部分元件。在圖3中，聚光單元180使多種色光（即第一色光LB、第二色光LG與第三色光LR的至少其中兩個）的合光聚焦在待測試管50上較高的位置。另一方面，在圖4中，聚光單元180使多種色光的合光聚焦在待測試管50上較低的位置。也就是，調整聚光單元180可以調整聚焦的位置。在調整聚焦的位置的過程中，可以找出良好的調整聚焦的位置，也就是三種感測器的感測效果良好的位置。FIG. 3 is a schematic diagram illustrating the function of the light concentrating unit of the thermal convective polymerase chain reaction apparatus of FIG. 1 . Please refer to FIG. 3. In this embodiment, the thermal convection polymerase chain reaction device 100 further includes a light concentrating unit 180, which is arranged between the light output region R12 of the light combining element 140 and the base 110, and is suitable for the first color At least two of the light LB, the second color light LG and the third color light LR are focused on the tube 50 to be tested, and are adapted to adjust the focus position. 4 is a schematic diagram of an adjusted light concentrating unit of the thermal convective polymerase chain reaction device in FIG. 3 , and some components of the thermal convective polymerase chain reaction device are omitted in FIG. 3 and FIG. 4 . In FIG. 3 , the light concentrating unit 180 focuses the combined light of multiple colored lights (ie at least two of the first colored light LB, the second colored light LG and the third colored light LR) on a higher position on the tube 50 to be tested. On the other hand, in FIG. 4 , the light concentrating unit 180 focuses the combined light of multiple colors of light on a lower position on the tube 50 to be tested. That is, adjusting the light-condensing unit 180 can adjust the focusing position. In the process of adjusting the focus position, a good focus adjustment position can be found, that is, a position where the sensing effects of the three sensors are good.

此外，聚光單元180可使聚焦的位置避開待測試管50的底部。由於待測試管50的底部容易沉積雜質，將聚焦的位置避開待測試管50的底部也可降低干擾並提高感測的精確度。聚焦的位置可以調整成位於待測試管50內的物質的上半部，以遠離待測試管50的底部。在本實施例中，聚光單元180包括一鏡筒182與一聚光透鏡184，聚光透鏡184可移動地設置於鏡筒182內，藉由鏡筒182與聚光透鏡184相對位置的移動，可以調整聚焦的位置。In addition, the light focusing unit 180 can make the focusing position avoid the bottom of the tube 50 to be tested. Since the bottom of the tube to be tested 50 is prone to deposit impurities, avoiding the focus position from the bottom of the tube to be tested 50 can also reduce interference and improve the accuracy of sensing. The position of the focus can be adjusted to be located in the upper half of the substance in the tube 50 to be tested, away from the bottom of the tube 50 to be tested. In this embodiment, the condensing unit 180 includes a lens barrel 182 and a condensing lens 184. The condensing lens 184 is movably arranged in the lens barrel 182. By moving the relative positions of the lens barrel 182 and the condensing lens 184 , you can adjust the focus position.

圖5是圖1的熱對流聚合酶連鎖反應裝置的合光元件的分解示意圖。圖6是圖5的合光元件的局部上視示意圖。請參照圖5與圖6，在本實施例中，相連的第一板狀部140A、第二板狀部140B、第三板狀部140C與第四板狀部140D呈X型結構。舉例而言，第一板狀部140A、第二板狀部140B、第三板狀部140C與第四板狀部140D彼此之間的夾角都是90度，但本揭露不侷限於此。在本實施例中，合光元件140更包括兩個固定座140E。第一板狀部140A、第二板狀部140B、第三板狀部140C與第四板狀部140D固定於兩個固定座140E之間。Fig. 5 is an exploded schematic view of the photo-synthetic element of the thermal convection polymerase chain reaction device in Fig. 1 . FIG. 6 is a schematic partial top view of the light combining element of FIG. 5 . Please refer to FIG. 5 and FIG. 6 , in this embodiment, the connected first plate-shaped portion 140A, second plate-shaped portion 140B, third plate-shaped portion 140C, and fourth plate-shaped portion 140D form an X-shaped structure. For example, the included angles between the first plate-shaped portion 140A, the second plate-shaped portion 140B, the third plate-shaped portion 140C, and the fourth plate-shaped portion 140D are all 90 degrees, but the present disclosure is not limited thereto. In this embodiment, the light combining element 140 further includes two fixing seats 140E. The first plate-shaped portion 140A, the second plate-shaped portion 140B, the third plate-shaped portion 140C and the fourth plate-shaped portion 140D are fixed between the two fixing seats 140E.

舉例而言，第一板狀部140A與第三板狀部140C可以具有相同的光學特性，也就是第一板狀部140A與第三板狀部140C都反射第一色光LB並允許第二色光LG與第三色光LR通過。第二板狀部140B與第四板狀部140D可以具有相同的光學特性，也就是第二板狀部140B與第四板狀部140D都反射第三色光LR並允許第二色光LG與第一色光LB通過。因此，本實施例的第一板狀部140A與第三板狀部140C可以是同一片光學元件，而第二板狀部140B與第四板狀部140D則是分開的兩片光學元件。這樣的設計只是為了組裝方便，但本揭露不侷限於此。For example, the first plate-shaped portion 140A and the third plate-shaped portion 140C may have the same optical characteristics, that is, both the first plate-shaped portion 140A and the third plate-shaped portion 140C reflect the first color light LB and allow the second color light LB to The color light LG and the third color light LR pass through. The second plate-shaped portion 140B and the fourth plate-shaped portion 140D may have the same optical characteristics, that is, both the second plate-shaped portion 140B and the fourth plate-shaped portion 140D reflect the third color light LR and allow the second color light LG to combine with the first color light. Shade LB through. Therefore, in this embodiment, the first plate-shaped portion 140A and the third plate-shaped portion 140C may be the same optical element, while the second plate-shaped portion 140B and the fourth plate-shaped portion 140D are two separate optical elements. Such a design is only for the convenience of assembly, but the present disclosure is not limited thereto.

在本實施例中，第一色光LB的波長例如是451.5nm至486.5 nm之間，第二色光LG的波長例如是507nm至527 nm之間，第三色光LR的波長例如是612 nm至644 nm之間。在另一實施例中，第一色光LB的波長例如是506nm至534 nm之間，第二色光LG的波長例如是543nm至566 nm之間，第三色光LR的波長例如是672nm至696 nm之間，依據上述區間的色光範圍照射試管50內的物質激發而產生的第一激發光EB例如適於檢測A型流感病毒，第二激發光EG例如適於檢測B型流感病毒，第三激發光ER例如適於檢測Covid-19病毒。In this embodiment, the wavelength of the first color light LB is, for example, between 451.5 nm and 486.5 nm, the wavelength of the second color light LG is, for example, between 507 nm and 527 nm, and the wavelength of the third color light LR is, for example, 612 nm to 644 nm. between nm. In another embodiment, the wavelength of the first color light LB is, for example, between 506 nm and 534 nm, the wavelength of the second color light LG is, for example, between 543 nm and 566 nm, and the wavelength of the third color light LR is, for example, 672 nm to 696 nm. Among them, the first excitation light EB produced by irradiating the material in the test tube 50 according to the color light range of the above interval is suitable for detecting type A influenza virus, for example, the second excitation light EG is suitable for detecting type B influenza virus, and the third excitation light EG is suitable for detecting type B influenza virus. Photo-ER is for example suitable for detecting the Covid-19 virus.

綜上所述，在本揭露的熱對流聚合酶連鎖反應裝置及其光學偵測方法中，因為使用了合光元件，所以可以將多種色光合光後進行檢測，同時檢測多種目標物，進而減少檢體的用量而降低受測者的負擔。To sum up, in the thermal convection polymerase chain reaction device and its optical detection method disclosed in the present disclosure, because of the use of a light-synthesizing element, it is possible to detect multiple colors of light after combining them, and detect multiple targets at the same time, thereby reducing The amount of sample used reduces the burden on the subject.

50:待測試管 60:光譜儀 100:熱對流聚合酶連鎖反應裝置 110:座體 112:細長通孔 130:加熱元件 140:合光元件 140A:第一板狀部 140B:第二板狀部 140C:第三板狀部 140D:第四板狀部 140E:固定座 150B:第一發光元件 150G:第二發光元件 150R:第三發光元件 150B1,150G1,150R1:發光二極體 150B2,150G2,150R2:濾光器 160B:第一光感測器 160G:第二光感測器 160R:第三光感測器 162B:第一濾光元件 162G:第二濾光元件 162R:第三濾光元件 170:電路板 180:聚光單元 182:鏡筒 184:聚光透鏡 A10,A20:長軸 R12:出光區 R14:第一入光區 R16:第二入光區 R18:第三入光區 LB:第一色光 LG:第二色光 LR:第三色光 EB:第一激發光 EG:第二激發光 ER:第三激發光 50: tube to be tested 60: spectrometer 100: Thermal Convection Polymerase Chain Reaction Apparatus 110: seat body 112: Slender through hole 130: heating element 140: Combined light element 140A: the first plate-shaped part 140B: the second plate-shaped part 140C: the third plate-shaped part 140D: the fourth plate-shaped part 140E: fixed seat 150B: the first light emitting element 150G: Second light-emitting element 150R: The third light-emitting element 150B1, 150G1, 150R1: Light-emitting diodes 150B2, 150G2, 150R2: optical filter 160B: the first light sensor 160G: Second light sensor 160R: The third light sensor 162B: the first filter element 162G: Second filter element 162R: The third filter element 170: circuit board 180: Concentrating unit 182: lens barrel 184: Concentrating lens A10, A20: long axis R12: light exit area R14: the first light incident zone R16: The second light entrance area R18: The third light entrance area LB: first shade LG: second color light LR: third color light EB: first excitation light EG: second excitation light ER: third excitation light

圖1是依照本揭露的一實施例的一種熱對流聚合酶連鎖反應裝置的立體剖視圖。圖2是圖1的熱對流聚合酶連鎖反應裝置的局部上視示意圖。圖3是說明圖1的熱對流聚合酶連鎖反應裝置的聚光單元的作用的示意圖。圖4是圖3的熱對流聚合酶連鎖反應裝置的聚光單元經調整後的示意圖。圖5是圖1的熱對流聚合酶連鎖反應裝置的合光元件的分解示意圖。圖6是圖5的合光元件的局部上視示意圖。 FIG. 1 is a perspective cross-sectional view of a thermal convection polymerase chain reaction device according to an embodiment of the present disclosure. Fig. 2 is a schematic partial top view of the thermal convection polymerase chain reaction device in Fig. 1 . FIG. 3 is a schematic diagram illustrating the function of the light concentrating unit of the thermal convective polymerase chain reaction apparatus of FIG. 1 . FIG. 4 is an adjusted schematic diagram of the light concentrating unit of the thermal convection polymerase chain reaction device in FIG. 3 . Fig. 5 is an exploded schematic view of the photo-synthetic element of the thermal convection polymerase chain reaction device in Fig. 1 . FIG. 6 is a schematic partial top view of the light combining element of FIG. 5 .

50:待測試管 50: tube to be tested

60:光譜儀 60: spectrometer

100:熱對流聚合酶連鎖反應裝置 100: Thermal Convection Polymerase Chain Reaction Apparatus

110:座體 110: seat body

112:細長通孔 112: Slender through hole

130:加熱元件 130: heating element

140:合光元件 140: Combined light element

140A:第一板狀部 140A: the first plate-shaped part

140B:第二板狀部 140B: the second plate-shaped part

140C:第三板狀部 140C: the third plate-shaped part

140D:第四板狀部 140D: the fourth plate-shaped part

150B:第一發光元件 150B: the first light emitting element

150G:第二發光元件 150G: Second light-emitting element

150R:第三發光元件 150R: The third light-emitting element

150B1,150G1,150R1:發光二極體 150B1, 150G1, 150R1: Light-emitting diodes

150B2,150G2,150R2:濾光器 150B2, 150G2, 150R2: optical filter

160R:第三光感測器 160R: The third light sensor

162R:第三濾光元件 162R: The third filter element

170:電路板 170: circuit board

R12:出光區 R12: light exit area

R14:第一入光區 R14: the first light incident area

R16:第二入光區 R16: The second light entrance zone

R18:第三入光區 R18: The third light entrance area

LB:第一色光 LB: first shade

LG:第二色光 LG: second color light

LR:第三色光 LR: third color light

ER:第三激發光 ER: third excitation light

A10,A20:長軸 A10, A20: long axis

Claims

一種熱對流聚合酶連鎖反應裝置，包括：一座體，適於承載一待測試管；一加熱元件，安裝於該座體，以透過該座體加熱該待測試管；一合光元件，包括相連的一第一板狀部、一第二板狀部、一第三板狀部與一第四板狀部，其中該第一板狀部與該第二板狀部界定一出光區，該第二板狀部與該第三板狀部界定一第一入光區，該第三板狀部與該第四板狀部界定一第二入光區，該第一板狀部與該第四板狀部界定一第三入光區；至少二發光元件；以及至少二光感測器，其中，該至少二光感測器的數量對應於該至少二發光元件的數量，該至少二發光元件為一第一發光元件、一第二發光元件與一第三發光元件的至少其中兩個，該第一發光元件適於朝該第一入光區提供一第一色光，該第二發光元件適於朝該第二入光區提供一第二色光，該第三發光元件適於朝該第三入光區提供一第三色光，該第一板狀部適於反射該第一色光並允許該第二色光與該第三色光通過，該第二板狀部適於反射該第三色光並允許該第一色光與該第二色光通過，該第三板狀部適於反射該第一色光並允許該第二色光通過，該第四板狀部適於反射該第三色光並允許該第二色光通過，該第一色光、該第二色光與該第三色光的至少其中兩個進入該出光區並朝向該座體出光以照射該待測試管；以及該至少二光感測器為一第一光感測器、一第二光感測器與一第三光感測器的至少其中兩個，安裝於該座體，其中該第一光感測器適於接收該待測試管內的物質受該第一色光激發而產生的一第一激發光，該第二光感測器適於接收該待測試管內的物質受該第二色光激發而產生的一第二激發光，該第三光感測器適於接收該待測試管內的物質受該第三色光激發而產生的一第三激發光。 A thermal convection polymerase chain reaction device, comprising: a seat, suitable for carrying a tube to be tested; a heating element installed on the base to heat the tube to be tested through the base; A light-combining element, including a first plate-shaped part, a second plate-shaped part, a third plate-shaped part and a fourth plate-shaped part, wherein the first plate-shaped part and the second plate-shaped part Define a light exit area, the second plate-shaped portion and the third plate-shaped portion define a first light-incidence area, the third plate-shaped portion and the fourth plate-shaped portion define a second light-incidence area, the first The plate portion and the fourth plate portion define a third light incident area; at least two light emitting elements; and at least two light sensors, Wherein, the number of the at least two photosensors corresponds to the number of the at least two light-emitting elements, The at least two light-emitting elements are at least two of a first light-emitting element, a second light-emitting element, and a third light-emitting element, and the first light-emitting element is suitable for providing a first color light toward the first light incident region, The second light-emitting element is adapted to provide a second color light toward the second light-incident area, the third light-emitting element is adapted to provide a third color light toward the third light-incident area, and the first plate portion is adapted to reflect the The first color light allows the second color light and the third color light to pass through, the second plate portion is suitable for reflecting the third color light and allows the first color light and the second color light to pass through, and the third plate portion is suitable for reflecting the first color light and allowing the second color light to pass through, the fourth plate portion is suitable for reflecting the third color light and allowing the second color light to pass through, the first color light, the second color light and the first color light At least two of the three colors of light enter the light exit area and emit light toward the base to illuminate the tube to be tested; and The at least two light sensors are at least two of a first light sensor, a second light sensor and a third light sensor, installed on the base body, wherein the first light sensor The detector is adapted to receive a first excitation light generated by the substance in the tube to be tested excited by the first color light, and the second light sensor is adapted to receive the substance in the tube to be tested excited by the second color light A second excitation light is generated, and the third light sensor is suitable for receiving a third excitation light generated by the substance in the test tube excited by the third color light.

如請求項1所述的熱對流聚合酶連鎖反應裝置，其中該第一板狀部、該第二板狀部、該第三板狀部與該第四板狀部各以一表面與其他三者相連，以排列形成X型結構。The thermal convection polymerase chain reaction device according to claim 1, wherein each of the first plate-shaped part, the second plate-shaped part, the third plate-shaped part and the fourth plate-shaped part has a surface and the other three Those connected to form an X-shaped structure.

如請求項1所述的熱對流聚合酶連鎖反應裝置，更包括一聚光單元，設置於該合光元件的該出光區與該座體之間，適於將該第一色光、該第二色光與該第三色光的至少其中兩個聚焦在該待測試管上，且適於調整聚焦的位置。The thermal convection polymerase chain reaction device as described in claim 1, further comprising a light concentrating unit, which is arranged between the light emitting area of the light synthesizing element and the base, and is suitable for the first color light, the second color light At least two of the dichromatic light and the third color light are focused on the tube to be tested, and are suitable for adjusting the focusing position.

如請求項3所述的熱對流聚合酶連鎖反應裝置，其中該聚光單元包括一鏡筒與一聚光透鏡，該聚光透鏡可移動地設置於該鏡筒內。The thermal convection polymerase chain reaction device as claimed in claim 3, wherein the light concentrating unit includes a lens barrel and a focus lens, and the focus lens is movably disposed in the lens barrel.

如請求項1所述的熱對流聚合酶連鎖反應裝置，其中該合光元件更包括兩個固定座，該第一板狀部、該第二板狀部、該第三板狀部與該第四板狀部固定於該些固定座之間。The thermal convective polymerase chain reaction device as described in Claim 1, wherein the light synthesizing element further includes two fixing seats, the first plate-shaped part, the second plate-shaped part, the third plate-shaped part and the second plate-shaped part The four plate parts are fixed between the fixing seats.

如請求項1所述的熱對流聚合酶連鎖反應裝置，更包括至少二濾光元件，該至少二濾光元件的數量對應於該至少二光感測器的數量，該至少二濾光元件為一第一濾光元件、一第二濾光元件與一第三濾光元件的至少其中兩個，其中該第一濾光元件設置於該第一光感測器與該座體之間，該第二濾光元件設置於該第二光感測器與該座體之間，該第三濾光元件設置於該第三光感測器與該座體之間。The thermal convection polymerase chain reaction device as described in claim 1, further comprising at least two filter elements, the number of the at least two filter elements corresponds to the number of the at least two light sensors, and the at least two filter elements are At least two of a first filter element, a second filter element and a third filter element, wherein the first filter element is disposed between the first light sensor and the base, the The second filter element is arranged between the second light sensor and the base, and the third filter element is set between the third light sensor and the base.

如請求項1所述的熱對流聚合酶連鎖反應裝置，其中該座體具有多個細長通孔，該些細長通孔的長軸垂直於承載在該座體內的該待測試管的長軸，分別對應連通於該第一光感測器、該第二光感測器及該第三光感測器。The thermal convection polymerase chain reaction device as claimed in item 1, wherein the base has a plurality of elongated through holes, the long axes of the elongated through holes are perpendicular to the long axis of the tube to be tested carried in the base, Correspondingly communicated with the first light sensor, the second light sensor and the third light sensor respectively.

如請求項1所述的熱對流聚合酶連鎖反應裝置，其中每一該發光元件包括一發光二極體與一濾光器，該濾光器設置於該發光二極體與該合光元件之間。The thermal convection polymerase chain reaction device as claimed in item 1, wherein each light-emitting element includes a light-emitting diode and a filter, and the filter is arranged between the light-emitting diode and the light-combining element between.

一種光學偵測方法，是使用如請求項1所述的熱對流聚合酶連鎖反應裝置進行檢測，其包括：加熱該待測試管內的物質；提供該第一色光、該第二色光與該第三色光的至少其中兩個，並使用該合光元件進行合光，合光後照射該待測試管；以及感測該待測試管內的物質受該第一色光激發而產生的該第一激發光、受該第二色光激發而產生的該第二激發光以及受該第三色光激發而產生的該第三激發光的至少其中兩個。 An optical detection method is to use the thermal convection polymerase chain reaction device as described in claim 1 to detect, which includes: heating the substance in the tube to be tested; providing at least two of the first color light, the second color light, and the third color light, and using the light combination element to combine light, and irradiate the test tube after light combination; and Sensing the first excitation light generated when the substance in the tube to be tested is excited by the first color light, the second excitation light generated by being excited by the second color light, and the second excitation light generated by being excited by the third color light at least two of the third excitation lights.

如請求項9所述的光學偵測方法，更包括使用一聚光單元，將該第一色光、該第二色光與該第三色光的至少其中兩個的合光聚焦在該待測試管上，且調整該聚光單元以調整聚焦的位置。The optical detection method as described in Claim 9, further comprising using a light-gathering unit to focus combined light of at least two of the first color light, the second color light, and the third color light on the tube to be tested , and adjust the focusing unit to adjust the focus position.

如請求項9所述的光學偵測方法，更包括使用至少二濾光元件，對該第一激發光、該第二激發光以及該第三激發光的至少其中兩個進行濾光後再感測。The optical detection method as claimed in claim 9, further comprising using at least two filter elements to filter at least two of the first excitation light, the second excitation light, and the third excitation light before sensing Measurement.

如請求項9所述的光學偵測方法，更包括使用多個細長通孔對該第一激發光、該第二激發光以及該第三激發光的至少其中兩個進行准直後再感測。The optical detection method as claimed in claim 9 further includes using a plurality of elongated through holes to collimate at least two of the first excitation light, the second excitation light and the third excitation light before sensing.