TW202246755A - Measuring method and measuring system for microbubble dispersion liquid - Google Patents

Measuring method and measuring system for microbubble dispersion liquid Download PDF

Info

Publication number
TW202246755A
TW202246755A TW110119199A TW110119199A TW202246755A TW 202246755 A TW202246755 A TW 202246755A TW 110119199 A TW110119199 A TW 110119199A TW 110119199 A TW110119199 A TW 110119199A TW 202246755 A TW202246755 A TW 202246755A
Authority
TW
Taiwan
Prior art keywords
liquid
tested
magnetic field
microbubbles
solid particles
Prior art date
Application number
TW110119199A
Other languages
Chinese (zh)
Other versions
TWI807329B (en
Inventor
大平猛
Original Assignee
日商大平研究所股份有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 日商大平研究所股份有限公司 filed Critical 日商大平研究所股份有限公司
Priority to TW110119199A priority Critical patent/TWI807329B/en
Publication of TW202246755A publication Critical patent/TW202246755A/en
Application granted granted Critical
Publication of TWI807329B publication Critical patent/TWI807329B/en

Links

Images

Landscapes

  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

Provided are a measuring method and a measuring system of a microbubble dispersion liquid that are capable of properly measuring the characteristics of the microbubble dispersion liquid while distinguishing between fine particles in the dispersion liquid and differentiating them into microbubbles and solid particles. The measuring system includes a microcapillary to hold the liquid under test, a laser device that irradiates the liquid under test inside the microcapillary with laser light, a variable magnetic field applying device that applies a time-varying magnetic field to the liquid under test within the irradiation area with laser light, a digital microscope that detects scattered light generated from fine particles contained in the liquid under test by irradiation with laser light, and a measurement device that measures the characteristics of the liquid under test based on the brightness of scattered light detected by the digital microscope.

Description

微氣泡分散液之測量方法及測量系統Measuring method and measuring system of microbubble dispersion liquid

本發明關於微氣泡分散液之一測量方法及一測量系統。更具體而言，本發明關於測量含微氣泡之微氣泡分散液特性之一測量方法及一測量系統。The present invention relates to a measurement method and a measurement system of microbubble dispersion liquid. More specifically, the present invention relates to a measurement method and a measurement system for measuring the properties of a microbubble dispersion liquid containing microbubbles.

研究探討含有微氣泡之微氣泡分散液在各工業領域之應用。在微氣泡中，該等具奈米尺度粒徑之微氣泡（以下稱為“奈米級直徑氣泡”）具有能在水中長時間（例如數年）停留之特性，因為其在分散液中之運動主要為布朗運動。為此原因，廣泛研究有奈米級直徑氣泡分散之微氣泡分散液。參見如專利文獻1（日本未審查專利申請公開號2019-103958）。Research and discuss the application of microbubble dispersion containing microbubbles in various industrial fields. Among the microbubbles, these microbubbles with nanoscale particle size (hereinafter referred to as "nanoscale diameter bubbles") have the characteristic of being able to stay in water for a long time (for example, several years), because their The motion is mainly Brownian motion. For this reason, microbubble dispersions having nanometer diameter bubbles dispersed have been extensively studied. See eg Patent Document 1 (Japanese Unexamined Patent Application Publication No. 2019-103958).

可藉由該液體是否混濁來相對容易地確定微氣泡分散液中是否存在有微氣泡。相對地，由於奈米級直徑氣泡之粒徑與光波長大致相同或小於光波長，因此在一微氣泡分散液中無法用肉眼確認奈米級直徑氣泡存在與否。The presence or absence of microbubbles in a microbubble dispersion can be determined relatively easily by whether the liquid is cloudy or not. In contrast, since the particle size of the nano-sized bubbles is approximately the same as or smaller than the wavelength of light, it is impossible to confirm the presence or absence of nano-sized bubbles in a microbubble dispersion liquid with the naked eye.

此外，已知如動態光散射(dynamic light scattering)法及粒子軌跡追踪(particle trajectory tracking)法等技術係用以光學測量微粒子濃度及粒徑分佈之技術，該微粒子係在含有上述奈米尺度微粒子之分散液中進行布朗運動。在動態光散射法及粒子軌跡追蹤法中，微粒子之濃度及粒徑分佈被測量，透過微粒子分散之液體被以雷射光照射，且該微粒子之散射光被追踪。In addition, techniques such as dynamic light scattering and particle trajectory tracking are known to optically measure the concentration and particle size distribution of microparticles containing the aforementioned nanoscale microparticles. Brownian motion in the dispersion. In the dynamic light scattering method and the particle trajectory tracking method, the concentration and particle size distribution of fine particles are measured, the liquid dispersed through the fine particles is irradiated with laser light, and the scattered light of the fine particles is tracked.

[專利文獻1]  日本未審查專利申請公開號2019-103958。[Patent Document 1] Japanese Unexamined Patent Application Publication No. 2019-103958.

[技術問題][technical problem]

在一些情況下，微氣泡分散液不僅含有奈米級直徑氣泡還含有奈米尺度微固體顆粒等雜質。然而，常用來測定含有分散微粒子液體中微粒子之方法雖然能夠識別出該液體中之奈米尺度微粒子，但該方法無法區分奈米級直徑氣泡及固體顆粒。In some cases, the microbubble dispersion contains not only nanoscale diameter bubbles but also impurities such as nanoscale microsolid particles. However, although the methods commonly used to measure particles in a liquid containing dispersed particles can identify nanoscale particles in the liquid, this method cannot distinguish nanoscale diameter bubbles from solid particles.

本發明一目的係提供一測量方法及一測量系統，其能夠區分出分散液中之微粒子，並將其區分成微氣泡及固體顆粒，從而正確地測量一微氣泡分散液之特性。 [問題解決方案] An object of the present invention is to provide a measurement method and a measurement system, which can distinguish the microparticles in the dispersion liquid, and distinguish them into microbubbles and solid particles, so as to accurately measure the characteristics of a microbubble dispersion liquid. [problem solution]

(1) 一種根據本發明測量一微氣泡分散液之方法，其係用以測量一待測液體特性之方法且該待測液體係一微氣泡分散液，其中該方法包括以下步驟：以一照明光照射一盛裝裝置中所盛裝之該待測液體，在該照明光之照射區域內對該待測液體施加一時變磁場，使用一光檢測裝置檢測藉該照明光照射而由該待測液體中所含複數個微粒子產生之一散射光；及藉由光檢測裝置根據測得之該散射光亮度區分該微粒子並將其區別為微氣泡及固體顆粒。(1) A method for measuring a microbubble dispersion according to the present invention, which is a method for measuring the properties of a liquid to be tested and the liquid to be tested is a microbubble dispersion, wherein the method includes the following steps: Light irradiates the liquid to be tested contained in a containing device, applies a time-varying magnetic field to the liquid to be tested in the irradiation area of the illuminating light, and uses a light detection device to detect The plurality of micro-particles contained in the micro-particles generate a scattered light; and the micro-particles are distinguished according to the brightness of the scattered light measured by the light detection device and are distinguished into micro-bubbles and solid particles.

(2) 在此情況下，較佳地該待測液體含有粒徑範圍2 nm至2000 nm之微氣泡。(2) In this case, preferably, the liquid to be tested contains microbubbles with a particle size ranging from 2 nm to 2000 nm.

(3) 在此情況下，較佳地藉由比較光檢測裝置獲得之該等微粒子的一影像亮度與一預定閾值來將該等微粒子區別成固體顆粒及微氣泡。(3) In this case, it is preferable to distinguish the fine particles into solid particles and microbubbles by comparing an image brightness of the fine particles obtained by the light detection means with a predetermined threshold.

(4) 在此情況下，較佳地在檢測該散射光之步驟中，在施加可變磁場前後以該光檢測裝置測得之該散射光來獲得該微粒子布朗運動軌跡之一影像，其中該光檢測裝置所獲得之該影像中，根據對該待測液體施加該可變磁場時亮度是否有增加而將該等微粒子識別成固體顆粒及微氣泡。(4) In this case, preferably in the step of detecting the scattered light, use the scattered light measured by the light detection device before and after applying the variable magnetic field to obtain an image of the Brownian motion trajectory of the microparticle, wherein the In the image obtained by the light detection device, according to whether the brightness increases when the variable magnetic field is applied to the liquid to be tested, the microparticles are identified as solid particles and microbubbles.

(5) 在此情況下，較佳地在以該光檢測裝置獲得之該影像中，對該待測液體施加該可變磁場時，亮度增加之該等微粒子被識別為固體顆粒，而將消失之該等微粒子被識別為帶電微氣泡，而將亮度未增加之微粒子被識別為不帶電微氣泡。(5) In this case, preferably in the image obtained by the light detection device, when the variable magnetic field is applied to the liquid to be tested, the microparticles with increased brightness are identified as solid particles and will disappear Those fine particles were identified as charged microbubbles, and the fine particles whose brightness did not increase were identified as uncharged microbubbles.

(6) 在此情況下，較佳地該測量方法進一步包括測量固體顆粒，其中該待測液體中所含之固體顆粒濃度或粒徑分佈係根據該光檢測裝置所獲得之一影像計算得到。(6) In this case, preferably, the measurement method further includes measuring solid particles, wherein the concentration or particle size distribution of solid particles contained in the liquid to be measured is calculated based on an image obtained by the light detection device.

(7) 在此情況下，較佳地該測量方法進一步包括測量不帶電微氣泡，其中該待測液體中所含之不帶電微氣泡濃度或粒徑分佈係根據該光檢測裝置獲得之一影像計算得出。(7) In this case, preferably the measurement method further includes measuring uncharged microbubbles, wherein the concentration or particle size distribution of uncharged microbubbles contained in the liquid to be tested is based on an image obtained by the photodetection device Calculated.

(8) 在此情況下，較佳地該測量方法進一步包括測量帶電微氣泡，其中在檢測散射光之步驟中獲得以下二者：對該液體施加該可變磁場前該微粒子布朗運動軌跡之施加前影像，及對該液體施加該可變磁場時該等微粒子布朗運動軌跡之施加過程影像，而該待測液體中所含之帶電微氣泡濃度及粒徑分佈之至少任一者係根據該等影像計算得到。(8) In this case, preferably the measuring method further includes measuring charged microbubbles, wherein the following two are obtained in the step of detecting scattered light: the application of the Brownian motion trajectory of the microparticles before the variable magnetic field is applied to the liquid The front image, and the application process image of the particle Brownian motion trajectory when the variable magnetic field is applied to the liquid, and at least any one of the charged microbubble concentration and particle size distribution contained in the liquid to be tested is based on the The image is calculated.

(9) 在此情況下，較佳地該測量方法進一步包括進行該待測液體之第一次篩選，其中在以該盛裝裝置盛裝該待測液體前，該待測液體已通過帶正電荷之過濾器。(9) In this case, preferably the measurement method further includes performing the first screening of the liquid to be tested, wherein the liquid to be tested has passed through a positively charged filter.

(10) 在此情況下，較佳地將可變磁場施加至該液體時根據散射光亮度之變化程度，使用光檢測裝置獲得微粒子之影像來計算該固體顆粒之粒徑。(10) In this case, it is preferable to calculate the particle size of the solid particle by using a light detection device to obtain an image of the particle according to the change degree of the brightness of the scattered light when a variable magnetic field is applied to the liquid.

(11) 在此情況下，較佳地該測量方法進一步包括對照明光之照明區域內之該待測液體施加一電場，在檢測散射光之步驟中，在對該待測液體施加該電場時獲得該微粒子電泳軌跡之一施加過程影像，並根據該施加過程影像測量該待測液體中所含之固體顆粒性質。(11) In this case, preferably the measurement method further includes applying an electric field to the liquid to be tested in the illumination area of the illumination light, and in the step of detecting scattered light, when the electric field is applied to the liquid to be tested to obtain An application process image of the particle electrophoresis track, and the property of the solid particles contained in the liquid to be tested is measured according to the application process image.

(12) 在此情況下，較佳地該測量方法進一步包括進行該待測液體之第二次篩選，其中對該待測液體施加可變磁場前，對該液體施加一靜磁場，藉此將至少將一部分之固體顆粒移出該照射區域。(12) In this case, preferably, the measurement method further includes performing a second screening of the liquid to be tested, wherein before applying a variable magnetic field to the liquid to be tested, a static magnetic field is applied to the liquid, whereby the At least a portion of the solid particles are removed from the irradiated area.

(13) 在此情況下，較佳地該測量方法進一步包括測量順磁性物質之量，其中順磁性固體顆粒數量被測量，該固體顆粒係在進行第二次篩選步驟中藉由對該待測液體施加該靜磁場來收集。(13) In this case, it is preferable that the measurement method further includes measuring the amount of paramagnetic substance, wherein the amount of paramagnetic solid particles is measured, and the solid particles are obtained by the test in the second screening step. The liquid applies this static magnetic field to collect.

(14) 在此情況下，較佳地該照明光之光源係一雷射裝置，而該雷射裝置能夠藉由使用界定範圍在300 nm至700 nm內之二種以上波長值來切換雷射光之波長。(14) In this case, it is preferable that the light source of the illumination light is a laser device capable of switching the laser light by using two or more wavelength values within the defined range of 300 nm to 700 nm the wavelength.

(15) 一種根據本發明用於測量一微氣泡分散液之測量系統，其係一種用以測量一待測液體特性之系統且該待測液體係一微氣泡分散液，其中該系統包括：一盛裝裝置，其盛裝一待測液體；一光源，其以照明光照射該盛裝裝置所盛裝之該待測液體；一可變磁場施加裝置，其在該照明光照射區域內對該待測液體施加一時變磁場；一光檢測裝置，其檢測該照明光照射下由該待測液體中所含之微粒子產生之該散射光；及一測量裝置，其測量該待測液體特性，根據該光檢測裝置所測得之散射光亮度區分該微粒子並將其區別成微氣泡及固體顆粒。 [本發明之有益效果] (15) A measurement system for measuring a microbubble dispersion according to the present invention, which is a system for measuring a property of a liquid to be tested and the liquid to be tested is a microbubble dispersion, wherein the system includes: a Containing device, which contains a liquid to be tested; a light source, which irradiates the liquid to be tested contained in the containing device with illuminating light; A time-varying magnetic field; a light detection device, which detects the scattered light generated by the microparticles contained in the liquid to be tested under the illumination of the illumination light; and a measuring device, which measures the characteristics of the liquid to be tested, according to the light detection device The measured brightness of the scattered light differentiates the microparticles and differentiates them into microbubbles and solid particles. [Beneficial effects of the present invention]

(1) 根據本發明之微氣泡分散液測量方法包括以下步驟：以照明光照射一盛裝裝置所盛裝之該待測液體；在該照明光照射區域內對該待測液體施加一時變磁場；及使用一光檢測裝置發射照明光來檢測該待測液體中所含微粒子產生之散射光。如上所述，該待測液體(其為一微氣泡分散液)可含有固體顆粒及微氣泡。若一可變磁場被施加至如此一待測液體，具有偏導磁性(biased magnetic permeability)之該固體顆粒相較於球形顆粒(在導磁性方面係以重心作為其中心)在一可變磁場下可承受更大之旋轉或平移力矩(rotational or translational moment of force)，因此前者會繞軸旋轉。相對地，微氣泡(在導磁性方面重心在其中心之球狀顆粒)相較於固體顆粒在一可變磁場下承受較小之旋轉或平移力矩。此外，相較於固體顆粒，幾乎無導磁性之該微氣泡重量非常小，受周圍介質產生很大之黏性阻力因此微氣泡幾乎不繞軸旋轉。在以照明光照射被施加一可變磁場之該待測液體時，繞軸旋轉之固體顆粒之散射光亮度比不繞軸旋轉之微氣泡之散射光亮度增加更多。因此，本發明之測量方法根據光檢測裝置測得之散射光亮度來區分待測液體中之微氣泡及固體顆粒。據此，本發明測量方法可以高準確度地測量該待測液體特性，同時區分待測液體中所含之微粒子並將其區別成微氣泡及固體顆粒。(1) The microbubble dispersion liquid measuring method according to the present invention comprises the following steps: irradiating the liquid to be tested contained in a container with illuminating light; applying a time-varying magnetic field to the liquid to be tested in the area irradiated by the illuminating light; and A light detecting device is used to emit illuminating light to detect the scattered light produced by the microparticles contained in the liquid to be tested. As mentioned above, the liquid to be tested (which is a microbubble dispersion) may contain solid particles and microbubbles. If a variable magnetic field is applied to such a liquid to be tested, the solid particles with biased magnetic permeability, compared with spherical particles (which have the center of gravity as their center in terms of magnetic permeability) will be under a variable magnetic field. It can withstand greater rotational or translational moment of force, so the former will rotate around the axis. In contrast, microbubbles (spherical particles with a center of gravity at their center in terms of magnetic permeability) experience smaller rotational or translational moments under a variable magnetic field than solid particles. In addition, compared with solid particles, the microbubbles, which have almost no magnetic permeability, have a very small weight, and are subject to great viscous resistance from the surrounding medium, so the microbubbles hardly rotate around their axes. When the liquid to be tested is irradiated with illumination light and applied with a variable magnetic field, the scattered light brightness of the solid particles rotating around the axis increases more than that of the microbubbles not rotating around the axis. Therefore, the measurement method of the present invention distinguishes microbubbles and solid particles in the liquid to be measured according to the brightness of scattered light measured by the light detection device. Accordingly, the measuring method of the present invention can measure the characteristics of the liquid to be tested with high accuracy, and at the same time distinguish the microparticles contained in the liquid to be tested and differentiate them into microbubbles and solid particles.

(2) 本發明之測量方法測量該待測液體，其包含粒徑範圍在2 nm至2000 nm之微氣泡。如上所述，已知測量方法無法區分奈米級直徑氣泡及相似粒徑之固體顆粒。相對地，即使微氣泡分散液含有與奈米級直徑氣泡相似粒徑之固體顆粒，本發明之測量方法可高準確度地測量該待測液體特性同時區分出該等固體顆粒及奈米級直徑氣泡。(2) The measurement method of the present invention measures the liquid to be tested, which contains microbubbles with a particle size ranging from 2 nm to 2000 nm. As mentioned above, known measurement methods cannot distinguish nanometer-sized diameter bubbles from solid particles of similar size. In contrast, even if the microbubble dispersion contains solid particles with a diameter similar to that of the nano-sized bubbles, the measurement method of the present invention can measure the properties of the liquid to be tested with high accuracy and distinguish between the solid particles and the nano-sized bubbles. bubble.

(3) 本發明之測量方法使用該光檢測裝置獲得之該微粒子影像藉由與一預定閾值比較其亮度而將微粒子區分成固體顆粒及微氣泡。如此就可以用簡單方法將該微粒子區別為固體顆粒及微氣泡。(3) The measurement method of the present invention distinguishes the microparticles into solid particles and microbubbles by comparing the brightness of the microparticle image obtained by the photodetection device with a predetermined threshold. In this way, the microparticles can be distinguished into solid particles and microbubbles in a simple manner.

(4) 在本發明之測量方法中，在檢測散射光之步驟中，在對該待測液體施加一可變磁場前後以該光檢測裝置獲得該微粒子之布朗運動軌跡影像，且在以該光檢測裝置獲得之影像中，根據對該液體施加之該可變磁場時其亮度是否增加來將該微粒子區分為固體顆粒及微氣泡。因此，該方法在追踪該微粒子因布朗運動所致之運動時，可區分出微粒子為固體顆粒或微氣泡。(4) In the measurement method of the present invention, in the step of detecting scattered light, the Brownian motion trajectory image of the microparticle is obtained by the light detection device before and after applying a variable magnetic field to the liquid to be measured, and In the image obtained by the detection device, according to whether the brightness increases when the variable magnetic field is applied to the liquid, the microparticles are classified into solid particles and microbubbles. Therefore, the method can distinguish the microparticles as solid particles or microbubbles when tracking the movement of the microparticles due to Brownian motion.

(5) 該微氣泡分散液中所含之該微粒子被分成微固體顆粒及微氣泡，而該微氣泡被分成帶正電或帶負電微氣泡與很少或無電荷之不帶電微氣泡。如上所述，若對該待測液體施加一可變磁場，具偏導磁性之該固體顆粒繞軸旋轉，該帶電微氣泡消失，而該不帶電微氣泡不會繞軸旋轉。據此，在本發明測量方法中，施加該可變磁場時亮度增加之微粒子被識別為固體顆粒，對該液體施加該可變磁場時消失之微粒子被識別為帶電微氣泡，而對該液體施加該可變磁場時亮度未增加之微粒子被識別為不帶電微氣泡。因此，本發明測量方法可以高準確度地測量該待測液體特性同時區分該待測液體中所含之該微粒子並將其區別為固體顆粒、帶電微氣泡及不帶電微氣泡。(5) The microparticles contained in the microbubble dispersion liquid are divided into microsolid particles and microbubbles, and the microbubbles are divided into positively or negatively charged microbubbles and uncharged microbubbles with little or no charge. As mentioned above, if a variable magnetic field is applied to the liquid to be tested, the solid particles with partial magnetic properties rotate around the axis, the charged microbubbles disappear, and the uncharged microbubbles do not rotate around the axis. Accordingly, in the measurement method of the present invention, the microparticles whose brightness increases when the variable magnetic field is applied are identified as solid particles, and the microparticles that disappear when the variable magnetic field is applied to the liquid are identified as charged microbubbles. The microparticles whose luminance did not increase under the variable magnetic field were identified as uncharged microbubbles. Therefore, the measuring method of the present invention can measure the properties of the liquid to be tested with high accuracy and distinguish the microparticles contained in the liquid to be tested into solid particles, charged microbubbles and uncharged microbubbles.

(6) 本發明之測量方法包括以下步驟：測量固體顆粒，其中該待測液體中所含之固體顆粒濃度及粒徑分佈之至少任一者係根據該光檢測裝置所獲得之影像計算得到。因此，本發明之測量方法可高準確度地測量固體顆粒之濃度及/或粒徑分佈同時區分出該固體顆粒及微氣泡。(6) The measurement method of the present invention includes the following steps: measuring solid particles, wherein at least one of the solid particle concentration and particle size distribution contained in the liquid to be measured is calculated based on the image obtained by the light detection device. Therefore, the measurement method of the present invention can measure the concentration and/or particle size distribution of solid particles with high accuracy while distinguishing the solid particles and microbubbles.

(7) 本發明之測量方法包括以下步驟：測量不帶電微氣泡，其中該待測液體中所含之不帶電微氣泡濃度及粒徑分佈之至少任一者係根據該光檢測裝置所獲得之影像計算得到。因此，本發明之測量方法可高準確度地測量該不帶電微氣泡之濃度及/或粒徑分佈同時區分出該不帶電微氣泡、固體顆粒及帶電微氣泡。(7) The measurement method of the present invention includes the following steps: measuring uncharged microbubbles, wherein at least any one of the concentration and particle size distribution of uncharged microbubbles contained in the liquid to be tested is obtained according to the light detection device The image is calculated. Therefore, the measurement method of the present invention can measure the concentration and/or particle size distribution of the uncharged microbubbles with high accuracy while distinguishing the uncharged microbubbles, solid particles and charged microbubbles.

(8) 在本發明之測量方法中，在檢測散射光之步驟中獲得以下二者：對該液體施加該可變磁場前該微粒子布朗運動軌跡之施加前影像，及對該液體施加該可變磁場時該微粒子布朗運動軌跡之施加過程影像。本發明之測量方法亦包括測量帶電微氣泡之步驟，其中該待測液體中所含之帶電微氣泡濃度及粒徑分佈之至少任一者係根據該等影像計算得到。因此，本發明之測量方法可高準確度地測量該帶電微氣泡之濃度及/或粒徑分佈同時區分出該帶電微氣泡、固體顆粒及不帶電微氣泡。(8) In the measurement method of the present invention, the following two are obtained in the step of detecting scattered light: the pre-application image of the particle Brownian motion track before the variable magnetic field is applied to the liquid, and the variable magnetic field is applied to the liquid. The image of the application process of the Brownian motion track of the particle when the magnetic field is applied. The measurement method of the present invention also includes the step of measuring charged microbubbles, wherein at least any one of the concentration and particle size distribution of charged microbubbles contained in the liquid to be tested is calculated based on the images. Therefore, the measurement method of the present invention can measure the concentration and/or particle size distribution of the charged microbubbles with high accuracy and distinguish the charged microbubbles, solid particles and uncharged microbubbles.

(9)專利文獻1（日本未審查專利申請公開號2019-103958）中確認在微氣泡分散液之微粒子，尤其是帶正電微氣泡具有促進植物生長之作用。相對地，在本發明之測量方法中，該待測液體被盛裝在一盛裝裝置前，該待測液體已通過一帶正電過濾器。結果，由於該待測液體所含之帶電微氣泡中帶負電微氣泡已被去除，本發明之測量方法可高準確度地測量帶正電微氣泡之濃度及/或粒徑分佈。(9) Patent Document 1 (Japanese Unexamined Patent Application Publication No. 2019-103958) confirms that the microparticles in the microbubble dispersion liquid, especially the positively charged microbubbles, have the effect of promoting plant growth. In contrast, in the measuring method of the present invention, the liquid to be tested is contained before a containing device, and the liquid to be tested has passed through a positively charged filter. As a result, since the negatively charged microbubbles contained in the liquid to be tested have been removed, the measuring method of the present invention can measure the concentration and/or particle size distribution of the positively charged microbubbles with high accuracy.

(10) 對該待測液體施加可變磁場時，該固體顆粒之旋轉次數會與散射光亮度改變程度相關。對該液體施加可變磁場時，該固體顆粒之旋轉次數也會與該固體顆粒粒徑相關。因此，本發明使用所上所述之散射光亮度及該固體顆粒粒徑之變化程度相關性並根據用該光檢測裝置獲得之微粒子影像之散射光亮度之變化程度來計算固體顆粒之粒徑。因此，本發明之測量方法可簡單測量出固體顆粒之粒徑。(10) When a variable magnetic field is applied to the liquid to be tested, the number of rotations of the solid particles will be related to the change in the brightness of the scattered light. When a variable magnetic field is applied to the liquid, the number of rotations of the solid particles is also related to the size of the solid particles. Therefore, the present invention uses the above-mentioned correlation between the intensity of scattered light and the change degree of the particle size of the solid particle and calculates the particle size of the solid particle according to the change degree of the light intensity of the scattered light in the microparticle image obtained by the photodetection device. Therefore, the measurement method of the present invention can simply measure the particle diameter of solid particles.

(11) 本發明之測量方法在照明光照射區域內對該待測液體施加一電場獲得微粒子電泳軌跡影像，並測量該待測液體中所含之該固體顆粒性質。因此，本發明之測量方法可測量固體顆粒之電特性。(11) In the measurement method of the present invention, an electric field is applied to the liquid to be tested in the illuminated area to obtain an image of the electrophoretic trajectory of microparticles, and the properties of the solid particles contained in the liquid to be tested are measured. Therefore, the measurement method of the present invention can measure the electrical properties of solid particles.

(12) 本發明之測量方法在對該液體施加可變磁場前藉由施加一靜磁場使至少一部分該固體顆粒從該照射區域中移出。此使得該待測液體中所含之眾多固體顆粒中之順磁性固體顆粒移動到該照射區域外成為可能，進一步提高測量準確度。(12) The measurement method of the present invention removes at least a part of the solid particles from the irradiation area by applying a static magnetic field before applying a variable magnetic field to the liquid. This makes it possible for the paramagnetic solid particles among the solid particles contained in the liquid to be measured to move out of the irradiation area, further improving the measurement accuracy.

(13) 如上所述, 本發明之測量方法藉由施加一靜磁場使順磁性固體顆粒移出該照射區域外，並收集顆粒以測量順磁性固體顆粒之量。此使得該待測液體中所含之順磁性固體顆粒之量能以簡單方式測量。(13) As mentioned above, the measurement method of the present invention moves paramagnetic solid particles out of the irradiation area by applying a static magnetic field, and collects the particles to measure the amount of paramagnetic solid particles. This enables the amount of paramagnetic solid particles contained in the liquid to be tested to be measured in a simple manner.

(14) 對該待測液體施加可變磁場時，固體顆粒之旋轉次數取決於該固體顆粒之物理性質及形狀以及介質黏度。該散射光亮度取決於固體顆粒旋轉次數及照射光波長。因此，若照射光波長固定，則該散射光之亮度可能無法再增加或飽和。相對地，本發明使用可切換雷射光波長之雷射裝置，其藉由使用界定範圍在300 nm至700 nm範圍內之複數個波長值作為照射光之光源。因此，若由於其亮度無法再增加或飽和而無法測量散射光亮度，則可相應地切換該雷射光之波長從而可適當地測量出散射光亮度。(14) When a variable magnetic field is applied to the liquid to be tested, the number of rotations of the solid particles depends on the physical properties and shape of the solid particles and the viscosity of the medium. The brightness of the scattered light depends on the number of rotations of the solid particles and the wavelength of the irradiated light. Therefore, if the wavelength of the irradiated light is fixed, the brightness of the scattered light may no longer increase or be saturated. In contrast, the present invention uses a laser device that can switch the wavelength of laser light by using a plurality of wavelength values defined within the range of 300 nm to 700 nm as a light source for irradiating light. Therefore, if the brightness of the scattered light cannot be measured because the brightness cannot be increased or is saturated, the wavelength of the laser light can be switched accordingly so that the brightness of the scattered light can be properly measured.

(15) 一種本發明之微氣泡分散液之測量系統，其包括：一盛裝裝置，其盛裝一待測液體；一光源，其以照明光照射該盛裝裝置所盛裝之該待測液體；一可變磁場施加裝置，其在該照明光照射區域內對該待測液體施加一時變磁場；一光檢測裝置，其檢測該照明光照射下由該待測液體中所含之微粒子產生之散射光；及一測量裝置，其測量該待測液體特性，根據該光檢測裝置所測得之該散射光亮度區分該微粒子並將其區別成微氣泡及固體顆粒。因此，基於與本節(1)中所包括之發明之相同原因，該測量系統可高準確度地測量該待測液體特性，同時區分該待測液體中所含之微粒子並將其區別成微氣泡及固體顆粒。(15) A measurement system for the microbubble dispersion liquid of the present invention, which includes: a containing device containing a liquid to be tested; a light source that irradiates the liquid to be measured contained in the containing device with illuminating light; A variable magnetic field applying device, which applies a time-varying magnetic field to the liquid to be tested in the area irradiated by the illumination light; a light detection device, which detects the scattered light generated by the microparticles contained in the liquid to be tested under the illumination of the illumination light; and a measuring device, which measures the properties of the liquid to be tested, and distinguishes the microparticles into microbubbles and solid particles according to the brightness of the scattered light measured by the light detection device. Therefore, based on the same reason as the invention included in this section (1), the measurement system can measure the characteristics of the liquid to be tested with high accuracy, while distinguishing the microparticles contained in the liquid to be tested and distinguishing them into microbubbles and solid particles.

第一具體實施例First specific embodiment

以下結合圖式對一測量系統1配置進行詳細說明，其中根據第一具體實施例之微氣泡分散液測量方法施用至該測量系統1。The configuration of a measurement system 1 will be described in detail below with reference to the drawings, wherein the method for measuring microbubble dispersion liquid according to the first embodiment is applied to the measurement system 1 .

該測量系統 1被用以測量一待測液體之特性，其為一流體介質之微氣泡分散液，其中有分散之複數個微氣泡。該等微氣泡特性如分散在該待測液體中之粒徑分佈及微氣泡數濃度。如下文所述，該待測液體係一奈米氣泡分散液，其中粒徑範圍在2 nm至2000 nm之微氣泡及固體顆粒被分散在該流體介質中。以下，微氣泡及固體顆粒也被統稱為微粒子。此外，所使用之該流體介質為純水或氯化鈉(NaCl)溶液，但在本發明中所使用之該流體介質不限於此。而且，在以下所述之該測量系統1中，在所使用之該流體介質為不含無機離子純水之情況下，可特別高準確度地計算出微粒子之粒徑，但本發明中該流體介質不限於純水。所使用之該流體介質可以為任何流體，只要細小氣泡能在該流體介質中分散即可。The measurement system 1 is used to measure the characteristics of a liquid to be tested, which is a microbubble dispersion liquid in a fluid medium, in which there are dispersed plural microbubbles. The properties of the microbubbles such as particle size distribution and microbubble number concentration dispersed in the liquid to be tested. As described below, the liquid to be tested is a nanobubble dispersion, wherein microbubbles and solid particles with a particle size ranging from 2 nm to 2000 nm are dispersed in the fluid medium. Hereinafter, microbubbles and solid particles are also collectively referred to as fine particles. In addition, the fluid medium used is pure water or sodium chloride (NaCl) solution, but the fluid medium used in the present invention is not limited thereto. Moreover, in the measurement system 1 described below, when the fluid medium used is pure water without inorganic ions, the particle diameter of the microparticles can be calculated with high accuracy, but the fluid in the present invention The medium is not limited to pure water. The fluid medium used can be any fluid as long as the fine air bubbles can be dispersed in the fluid medium.

該測量系統1包括：一微毛細管2，作為一盛裝裝置以盛裝一待測液體；一雷射裝置3，作為以一雷射光L作為照明光來照射該微毛細管2中之該待測液體之光源；一可變磁場施加裝置5，其以該雷射光L對一照射區域A中之該待測液體施加一可變磁場；一數位顯微鏡6，作為一光檢測裝置，其檢測以該雷射光L照射該待測液體所含之微粒子所產生之一散射光S；一測量裝置7，其係根據該數位顯微鏡6獲得之影像資料來測量該待測液體特性之電腦；及一過濾裝置8，其過濾掉該待測液體中所含之雜質及其他物質。The measurement system 1 includes: a microcapillary 2, which is used as a containing device to contain a liquid to be measured; a laser device 3, which is used as a laser light L as an illumination light to irradiate the liquid to be measured in the microcapillary 2 Light source; a variable magnetic field applying device 5, which applies a variable magnetic field to the liquid to be tested in an irradiation area A with the laser light L; a digital microscope 6, as a photodetection device, which detects the laser light L L irradiates a scattered light S produced by the microparticles contained in the liquid to be measured; a measuring device 7, which is a computer for measuring the characteristics of the liquid to be measured according to the image data obtained by the digital microscope 6; and a filter device 8, It filters out impurities and other substances contained in the liquid to be tested.

如下文所述，具有如圖1所示之微米級導管作為盛裝裝置來盛裝該待測液體之該微毛細管2，但本發明之盛裝裝置不以此為限。作為一盛裝裝置，可接受任何能夠像該微毛細管2一樣藉由毛細現象來盛裝該待測液體之裝置。例如，可使用如下裝置：一導軌板(rail plate)21，在其上形成微米級寬度之溝槽(groove)(參見圖2A)；一成對之玻璃板22，以微米級間距堆疊(參見圖2B)；及以微米級間距排列之聚合物平行線(strings)23(參見圖2C)。As described below, the microcapillary 2 is provided with a micron-scale conduit as shown in FIG. 1 as a containment device for containing the liquid to be tested, but the containment device of the present invention is not limited thereto. As a containment device, any device capable of containing the liquid to be tested by capillary phenomenon like the microcapillary 2 is acceptable. For example, the following devices can be used: a rail plate (rail plate) 21 on which grooves (groove) with a micron-scale width are formed (see FIG. 2A); a pair of glass plates 22 are stacked with a micron-scale pitch (see Fig. 2B); and parallel polymer strings 23 (see Fig. 2C) arranged at micrometer pitches.

該雷射裝置3產生例如波長範圍在300 nm至700 nm之該雷射光L，並以該雷射光L照射被界定在該微毛細管2之毛細管內部之該照射區域A。較佳地所使用之該雷射裝置3可使用界定在300 nm至700 nm範圍內之二種以上波長值來切換該雷射光L波長。如下文所述，該雷射裝置3被用作光源，但本發明中之光源不限於此。例如，可使用發光二極體作為光源。The laser device 3 generates, for example, the laser light L with a wavelength ranging from 300 nm to 700 nm, and irradiates the irradiation area A defined inside the capillary of the microcapillary 2 with the laser light L. The laser device 3 preferably used can switch the wavelength of the laser light L by using more than two wavelength values defined within the range of 300 nm to 700 nm. As described below, the laser device 3 is used as a light source, but the light source in the present invention is not limited thereto. For example, light emitting diodes can be used as light sources.

該可變磁場施加裝置5包括由操作者握持操作之一線圈墊51及一主體52，該主體52在預定設置週期下從該線圈墊51產生隨時變化之一可變磁場之。在此該可變磁場施加裝置5中，可在預定範圍內設置該可變磁場之週期及磁通密度。因此，該可變磁場施加裝置5還可藉由將該可變磁場之週期設置成無限大來生成一靜磁場。如下文所述，藉由使用該可變磁場施加裝置5該可變磁場及該靜磁場均被選擇性地在該微毛細管2內以該雷射光L施加至該照射區域A，但本發明不限於此。具體地，作為產生該靜磁場之手段，可使用提前準備之獨立於該可變磁場施加裝置5之一磁體。The variable magnetic field applying device 5 includes a coil pad 51 held and operated by an operator and a main body 52. The main body 52 generates a variable magnetic field from the coil pad 51 at a predetermined setting period. In the variable magnetic field applying device 5 here, the period and the magnetic flux density of the variable magnetic field can be set within a predetermined range. Therefore, the variable magnetic field applying device 5 can also generate a static magnetic field by setting the period of the variable magnetic field to be infinite. As described below, both the variable magnetic field and the static magnetic field are selectively applied to the irradiation area A with the laser light L in the microcapillary 2 by using the variable magnetic field applying device 5, but the present invention does not limited to this. Specifically, as a means for generating the static magnetic field, a magnet prepared in advance and independent of the variable magnetic field applying device 5 can be used.

此外，如下文所述，作為用於產生該可變磁場之該可變磁場施加裝置5，使用MRS1000 (The Magstim Company Ltd., U.K.)但本發明不限於此。使用一引動器(未顯示)在該微毛細管2內藉由將該磁體移近或遠離該照射區域A來施加該可變磁場。In addition, as described below, as the variable magnetic field applying device 5 for generating the variable magnetic field, MRS1000 (The Magstim Company Ltd., U.K.) is used but the present invention is not limited thereto. The variable magnetic field is applied within the microcapillary 2 by moving the magnet closer to or farther away from the irradiation area A using an actuator (not shown).

此外，圖1顯示使用包括一個圓形線圈之一單線圈型線圈墊51之情況，但本發明不限於此。除該線圈墊 51外，如圖3中所示，使用包括二個圓形線圈511, 512之一雙線圈型線圈墊51A。該等線圈511及512略微傾斜。結果，相較於該單線圈型線圈墊51，該雙線圈型線圈墊51A能更好地提高磁通量之聚斂性，使得該照射區域A之磁通密度有選擇性地集中。Furthermore, FIG. 1 shows a case where a single-coil type coil pad 51 including one circular coil is used, but the present invention is not limited thereto. In addition to this coil pad 51, as shown in FIG. 3, a double coil type coil pad 51A including two circular coils 511, 512 is used. The coils 511 and 512 are slightly inclined. As a result, compared with the single-coil coil pad 51 , the double-coil coil pad 51A can better improve the convergence of magnetic flux, so that the magnetic flux density in the irradiation area A can be selectively concentrated.

該數位顯微鏡6包括：一光學系統(未顯示)設於該散射光S光路中 ，該散射光S係以該雷射光L在該微毛細管2內該照射區域A之該待測液體中所含之微粒子產生，其中該散射光S入射(incident)係被此光學系統接收；及一影像設備(未顯示)，其將該散射光S之亮度轉換為電子訊號來生成影像資料。該影像設備之例示為CCD或CMOS影像傳感器。以該數位顯微鏡6獲得之影像資料傳輸至該測量裝置7。The digital microscope 6 includes: an optical system (not shown) is located in the light path of the scattered light S, and the scattered light S is contained in the liquid to be tested in the irradiation area A in the microcapillary 2 by the laser light L wherein the scattered light S incident (incident) is received by the optical system; and an imaging device (not shown), which converts the brightness of the scattered light S into electronic signals to generate image data. An example of the imaging device is a CCD or a CMOS image sensor. The image data obtained by the digital microscope 6 is transmitted to the measuring device 7 .

該測量裝置7係有安裝一程式之電腦，該程式對微粒子影像資料進行數值處理以測量該待測液體特性(例如微粒子之粒徑分佈及粒數濃度，微粒子諸如分散在待測液體中之微氣泡及固體顆粒)。用於以此該測量裝置7測量該待測液體特性之特定步驟稍後將參考包括圖5及圖6之圖式進行描述。The measuring device 7 is a computer with a program installed. The program performs numerical processing on the microparticle image data to measure the characteristics of the liquid to be measured (such as the particle size distribution and particle number concentration of the microparticles, such as microparticles dispersed in the liquid to be tested. air bubbles and solid particles). Specific steps for measuring the properties of the liquid to be tested using the measuring device 7 will be described later with reference to the drawings including FIGS. 5 and 6 .

該過濾裝置8包括一過濾器主體，該過濾器主體設有許多具有預定一內徑之過濾孔。調節該過濾器主體之該過濾孔內徑，使該待測液體中之微粒子能夠通過，從而捕獲該待測液體中粒徑較大之固體顆粒。因此，允許該待測液體通過如此之該過濾器主體提前去除分散在該待測液體中粒徑大於該過濾孔內徑之固體顆粒。如下文所述，所使用之該過濾器主體帶正電，使得該過濾器主體也可收集分散在該待測液體中之微氣泡中帶負電之微氣泡，但本發明不限於此。所使用之該過濾器主體可能不帶電，也可能帶負電。The filter device 8 includes a filter body, and the filter body is provided with a plurality of filter holes with a predetermined inner diameter. The inner diameter of the filter hole of the filter main body is adjusted so that the microparticles in the liquid to be tested can pass through, thereby capturing solid particles with larger particle diameters in the liquid to be tested. Therefore, the liquid to be tested is allowed to pass through the filter body so that the solid particles dispersed in the liquid to be tested with a particle diameter larger than the inner diameter of the filter hole are removed in advance. As described below, the filter body used is positively charged so that the filter body can also collect negatively charged microbubbles among the microbubbles dispersed in the liquid to be tested, but the present invention is not limited thereto. The filter body used may be neutral or negatively charged.

圖4為藉由如上所述一測量系統1之用於測量該待測液體特性之測量方法之具體步驟流程圖。FIG. 4 is a flow chart of the specific steps of the measurement method for measuring the properties of the liquid to be measured by the above-mentioned measurement system 1 .

首先，在S1中，一操作員使一待測液體通過帶正電之一過濾器主體，其中該待測液體係一預先準備之微氣泡分散液。在此步驟中，分散在該待測液體中之該微粒子中具較大粒徑之該固體顆粒及該帶負電微氣泡被移除。被用作該待測液體之該微氣泡分散液可事先準備，或該待測液體可由一微氣泡產生器(未顯示)製造。First, in S1, an operator passes a liquid to be tested through a positively charged filter body, wherein the liquid to be tested is a pre-prepared microbubble dispersion. In this step, the solid particles with larger particle size and the negatively charged microbubbles among the microparticles dispersed in the liquid to be tested are removed. The microbubble dispersion used as the test liquid can be prepared in advance, or the test liquid can be produced by a microbubble generator (not shown).

然後，在S2中，該操作者使一微毛細管2盛裝該待測液體，其藉毛細現象通過該過濾器主體內部。Then, in S2, the operator makes a microcapillary 2 contain the liquid to be tested, which passes through the inside of the filter body by capillary phenomenon.

然後，在S3中，該操作者操作一可變磁場施加裝置5藉由設定該可變磁場週期為無限大以從一線圈墊51產生一靜磁場，並使該線圈墊51靠近該微毛細管2以對在該微毛細管2內之該待測液體施加該靜磁場。此外，在S3中，沿著該微毛細管2延伸方向掃描此該線圈墊51，以使得該微毛細管2內之該待測液體中一部分之固體顆粒(尤其為順磁性固體顆粒)移出一雷射光L之一照射區域A。如此處所述，使用該可變磁場施加裝置5對該待測液體施加該靜磁場，但施加該靜磁場之方法不限於此。例如，可使用與該可變磁場施加裝置5分開製備之磁鐵對一待測液體施加該靜磁場。對如上所述之該待測液體施加該靜磁場所收集到之固體顆粒數量可藉由已知方法單獨測得。Then, in S3, the operator operates a variable magnetic field applying device 5 to generate a static magnetic field from a coil pad 51 by setting the variable magnetic field cycle to be infinite, and makes the coil pad 51 close to the microcapillary 2 To apply the static magnetic field to the liquid to be tested in the microcapillary 2 . In addition, in S3, the coil pad 51 is scanned along the extending direction of the microcapillary 2, so that a part of the solid particles (especially paramagnetic solid particles) in the liquid to be measured in the microcapillary 2 move out a laser light One of L illuminates area A. As described here, the variable magnetic field applying device 5 is used to apply the static magnetic field to the liquid to be tested, but the method of applying the static magnetic field is not limited thereto. For example, a magnet prepared separately from the variable magnetic field applying device 5 can be used to apply the static magnetic field to a liquid to be tested. The number of solid particles collected by applying the static magnetic field to the liquid to be tested as described above can be independently measured by known methods.

然後，在S4中，該操作者用該雷射裝置3開始發射該雷射光L。更具體地，該操作者操作該雷射裝置3以產生該雷射光L，並用該雷射光L照射該微毛細管2內預先確定之該照射區域A。Then, in S4, the operator starts emitting the laser light L with the laser device 3 . More specifically, the operator operates the laser device 3 to generate the laser light L, and irradiates the predetermined irradiation area A in the microcapillary 2 with the laser light L.

然後，在S5中，該操作者開始用一數位顯微鏡6拍攝該照射區域A之動態影像。Then, in S5, the operator starts to take a dynamic image of the irradiation area A with a digital microscope 6 .

從該操作者在S5中所開始拍攝之動態影像起經過預定時間後，其次在S6中該操作者使用該可變磁場施加裝置5對該待測液體施加一可變磁場一段預定時間。該磁場變化周期為例如0.1秒，而該線圈墊51處該可變磁場之磁通密度為0.5‑2.0 T，但本發明不限於此。After a predetermined time elapses from the dynamic image that the operator starts to shoot in S5, then in S6, the operator uses the variable magnetic field applying device 5 to apply a variable magnetic field to the liquid to be tested for a predetermined time. The changing period of the magnetic field is, for example, 0.1 second, and the magnetic flux density of the variable magnetic field at the coil pad 51 is 0.5-2.0 T, but the present invention is not limited thereto.

然後，在S7中，該操作者用該可變磁場施加裝置5完成施加該可變磁場，然後用該數位顯微鏡6完成拍攝動態影像，並由該雷射裝置3發射該雷射光L。上述步驟提供布朗運動所致分散在該待測物體中之許多微粒子之運動圖像。如下文所述，該數位顯微鏡6拍攝施加該可變磁場前後之動態影像，但本發明不限於此。例如，可使用拍攝多個靜止影像之方法，其中分散在該待測液體中之微粒子之布朗運動軌跡可被追踪。Then, in S7 , the operator uses the variable magnetic field applying device 5 to finish applying the variable magnetic field, and then uses the digital microscope 6 to take a moving image, and the laser device 3 emits the laser light L. The above steps provide a moving image of many microparticles dispersed in the object to be measured due to Brownian motion. As described below, the digital microscope 6 captures dynamic images before and after applying the variable magnetic field, but the present invention is not limited thereto. For example, a method of taking multiple still images can be used, wherein the Brownian motion trajectory of the microparticles dispersed in the liquid to be tested can be tracked.

然後，在S8中，該操作者操作一測量裝置7以根據用該數位顯微鏡6獲得之該微粒子之動態影像資料計算該微粒子之濃度及粒徑分佈。Then, in S8, the operator operates a measuring device 7 to calculate the concentration and particle size distribution of the microparticles based on the dynamic image data of the microparticles obtained by the digital microscope 6 .

圖5係一流程圖，顯示用該測量裝置 7計算微粒子之濃度及粒徑分佈之步驟。該待測液體包含分散於其中之帶正電微氣泡、不帶電微氣泡及包括固體顆粒之奈米尺度微粒子，但動態光散射法及粒子軌跡追踪法等常用方法無法識別此類奈米尺度之微粒子。相對地，該測量裝置7可區分帶正電微氣泡、不帶電微氣泡及固體顆粒以計算出其各別之濃度及粒徑分佈，下文將詳細描述。以下，在說明圖5流程圖之具體步驟前，先參照圖6對使用該測量裝置7之測量方法進行概要描述。Fig. 5 is a flow chart showing the steps of calculating the concentration and particle size distribution of fine particles using the measuring device 7. The liquid to be tested contains positively charged microbubbles, uncharged microbubbles, and nanoscale particles including solid particles dispersed therein, but common methods such as dynamic light scattering and particle trajectory tracking methods cannot identify such nanoscale particles. microparticles. In contrast, the measuring device 7 can distinguish positively charged microbubbles, uncharged microbubbles and solid particles to calculate their respective concentrations and particle size distributions, which will be described in detail below. Hereinafter, before describing the specific steps of the flow chart in FIG. 5 , the measurement method using the measurement device 7 will be briefly described with reference to FIG. 6 .

圖6為用該數位顯微鏡6檢測雷射光散射光所獲得之微粒子影像例示圖。圖6提供部分微粒子之放大圖作為參考。FIG. 6 is an example diagram of microparticle images obtained by detecting scattered laser light with the digital microscope 6 . Figure 6 provides an enlarged view of some microparticles for reference.

如圖6所示，該待測液體包含分散於其中之不帶電微氣泡4a、帶電微氣泡4b及固體顆粒4c, 4d。而且，分散在該待測液體中之固體顆粒有各種形狀，包括具相對接近真球形之固體顆粒4c，及遠非真球之扭曲形狀之固體顆粒4d。然而，此等微粒子4a‒4d具有奈米尺度之粒徑，其等於或小於雷射光波長，使該等微粒子無法藉由檢測散射光獲得之影像來識別。然而，如圖6所示，用該可變磁場施加裝置5對該待測液體施加預定週期變化之時變磁場時，該微粒子有不同表現。As shown in FIG. 6, the liquid to be tested includes uncharged microbubbles 4a, charged microbubbles 4b and solid particles 4c, 4d dispersed therein. Moreover, the solid particles dispersed in the liquid to be tested have various shapes, including solid particles 4c which are relatively close to a true spherical shape, and solid particles 4d which are distorted shapes which are far from a true spherical shape. However, these microparticles 4a-4d have particle diameters in the nanometer scale, which are equal to or smaller than the wavelength of laser light, so that these microparticles cannot be identified by detecting images obtained from scattered light. However, as shown in FIG. 6 , when the variable magnetic field applying device 5 is used to apply a predetermined periodic time-varying magnetic field to the liquid to be tested, the microparticles behave differently.

更具體地，具偏導磁性之固體顆粒4c, 4d在可變磁場下承受旋轉或平移力而繞軸旋轉。在此情況下，相較於具扭曲形狀之固體顆粒4d，具相對接近真球形之固體顆粒4c被認為具較少黏性阻力，因此，固體顆粒4c比具扭曲形狀之固體顆粒4d旋轉更快。因此，若對固體顆粒施加可變磁場同時對其發射雷射光，固體顆粒4c, 4d之散射光亮度相較於施加可變磁場前增加更多。也在此時，由於固體顆粒4d旋轉速度大於固體顆粒4c，因此固體顆粒4d之亮度比固體顆粒4c之亮度增加更多。此外，認為隨著該固體顆粒4c, 4d之粒徑增加其亮度增加更多。More specifically, the solid particles 4c, 4d with deflection magnetism are subjected to rotational or translational forces under a variable magnetic field to rotate around their axes. In this case, the solid particle 4c having a relatively close true spherical shape is considered to have less viscous resistance compared to the solid particle 4d having a distorted shape, and therefore, the solid particle 4c rotates faster than the distorted shape solid particle 4d . Therefore, if a variable magnetic field is applied to the solid particles while emitting laser light, the brightness of the scattered light of the solid particles 4c, 4d increases more than before the variable magnetic field is applied. Also at this time, since the solid particle 4d rotates faster than the solid particle 4c, the brightness of the solid particle 4d increases more than that of the solid particle 4c. In addition, it is considered that the brightness of the solid particles 4c, 4d increases more as the particle size increases.

作為參考，以下公式(1)表示一球體之運動方程式，其中質量M (kg)及半徑r (m)之球體在μ (N•s/m 2)黏度係數介質中之磁矩為m (wb•m)，而對介質施加頻率為ω o(rad/s)之時變磁場H (A/m)。在式(1)中，t (s)為時間，θ(rad)係由磁矩m及磁場H所界定之角度。

Figure 02_image001
(1)     [式1] For reference, the following formula (1) represents the equation of motion of a sphere, where the magnetic moment of a sphere with mass M (kg) and radius r (m) in a medium with a viscosity coefficient of μ (N·s/m 2 ) is m (wb •m), and a time-varying magnetic field H (A/m) with a frequency of ω o (rad/s) is applied to the medium. In formula (1), t (s) is the time, θ (rad) is the angle defined by the magnetic moment m and the magnetic field H.
Figure 02_image001
(1) [Formula 1]

相對地，相較於固體顆粒，不帶電微氣泡4a幾乎不具導磁性且重量很小，其對周圍介質導致非常大之黏性阻力，從而即使對不帶電微氣泡4a施加可變磁場其也幾乎不旋轉。因此，即使對微氣泡4a施加可變磁場及發射雷射光，微氣泡4a之散射光亮度幾乎沒有增加。此外，若對帶電微氣泡4b施加可變磁場則其消失。據此，若對微氣泡4b施加可變磁場及發射雷射光，帶電微氣泡4b之散射光亮度為零。In contrast, compared to solid particles, the uncharged microbubbles 4a have almost no magnetic permeability and are very light in weight, which causes very large viscous resistance to the surrounding medium, so that even if a variable magnetic field is applied to the uncharged microbubbles 4a, it is almost Does not rotate. Therefore, even if a variable magnetic field is applied to the microbubble 4a and laser light is emitted, the brightness of the scattered light of the microbubble 4a hardly increases. In addition, when a variable magnetic field is applied to the charged microbubbles 4b, they disappear. Accordingly, if a variable magnetic field is applied to the microbubble 4b and laser light is emitted, the brightness of the scattered light of the charged microbubble 4b is zero.

利用若對微粒子4a‒4d施加可變磁場則各個微粒子有不同表現之事實，該測量裝置7可區分微粒子4a‒4d。The measuring device 7 can differentiate the microparticles 4a-4d by utilizing the fact that the microparticles 4a-4d behave differently if a variable magnetic field is applied to them.

以下對圖5之流程圖進行說明。首先，在S11中，根據在施加該可變磁場前後該微粒子布朗運動軌跡之視訊影像，該測量裝置7將視訊影像中所含之微粒子區分為不帶電微氣泡、帶電微氣泡及固體顆粒。更具體地，該測量裝置7區分出固體顆粒、帶電微氣泡及不帶電微氣泡，以使得該測量裝置7在施加該可變磁場時將該等亮度增加幅度大於預定閾值之微粒子識別為該固體顆粒，在施加該可變磁場時將已消失、亮度變為零之微粒子識別為帶電微氣泡，以及在施加該可變磁場時將亮度增加幅度未大於預定閾值之微粒子識別為該不帶電微氣泡。The flow chart of Fig. 5 will be described below. Firstly, in S11, according to the video image of the Brownian motion trajectory of the particle before and after the application of the variable magnetic field, the measurement device 7 distinguishes the microparticles contained in the video image into uncharged microbubbles, charged microbubbles and solid particles. More specifically, the measuring device 7 distinguishes solid particles, charged microbubbles and uncharged microbubbles, so that the measuring device 7 recognizes those microparticles whose luminance increases greater than a predetermined threshold value as the solid particles when the variable magnetic field is applied. Particles, when the variable magnetic field is applied, the microparticles that have disappeared and the brightness becomes zero are identified as charged microbubbles, and the microparticles whose brightness is not increased by more than a predetermined threshold are identified as the uncharged microbubbles when the variable magnetic field is applied .

在此S11中，在施加該可變磁場時若一散射光S亮度無法再增加或飽和，較佳地該測量裝置 7中止後續之計算，並在改變該雷射裝置3之雷射光波長後再次執行圖4所示步驟。In this S11, if the brightness of a scattered light S cannot be increased or saturated when the variable magnetic field is applied, preferably the measuring device 7 suspends subsequent calculations and restarts after changing the wavelength of the laser light of the laser device 3. Perform the steps shown in Figure 4.

然後，在S12中，在施加該可變磁場前該測量裝置7在S11中微粒子布朗運動軌跡動態影像中統計出識別出之該不帶電微氣泡、該帶電微氣泡及該固體顆粒各自之數量，藉此該測量裝置7計算出該待測液體中不帶電微氣泡粒數濃度C bo、帶電微氣泡粒數濃度C bc以及固體顆粒粒數濃度C 固體。 Then, in S12, before applying the variable magnetic field, the measuring device 7 counts the respective numbers of the identified uncharged microbubbles, the charged microbubbles and the solid particles in the dynamic image of the Brownian motion trajectory of the microparticles in S11, Thereby the measuring device 7 calculates the number concentration of uncharged microbubbles C bo , the number concentration of charged microbubbles C bc and the number concentration of solid particles C solid in the liquid to be tested.

然後，在S13中，在施加該可變磁場前該測量裝置7根據該微粒子布朗運動軌跡動態影像分析該微粒子布朗運動之軌跡，藉此該測量裝置7分別由該不帶電微氣泡、該帶電微氣泡及該固體顆粒計算出在S12所統計數量之各微粒子之粒徑。更具體地，由於分散該待測物體中之該微粒子之布朗運動速度會隨著該微粒子粒徑變大而變慢，以及隨著該微粒子粒徑變小而變快。藉由分析如上所述微粒子布朗運動軌跡，可計算出每一微粒子之粒徑。Then, in S13, before applying the variable magnetic field, the measurement device 7 analyzes the Brownian motion trajectory of the microparticles according to the dynamic image of the Brownian motion trajectory of the microparticles, whereby the measurement device 7 detects the uncharged microbubbles and the charged microbubbles respectively. Air bubbles and the solid particles are used to calculate the particle diameters of the microparticles counted in S12. More specifically, the Brownian motion velocity of the microparticles dispersed in the object to be measured becomes slower as the particle size becomes larger, and becomes faster as the particle size becomes smaller. The particle size of each particle can be calculated by analyzing the Brownian motion trajectory of the above-mentioned particles.

然後，在S14中，使用在S13中由該不帶電微氣泡、該帶電微氣泡及該固體顆粒計算出之每一微粒子之粒徑資訊，該測量裝置7計算出分散在該待測液體中之該不帶電微氣泡之粒徑分佈D bo，該帶電微氣泡之粒徑分佈 D bc，及該固體顆粒之粒徑分佈D 固體。 第二具體實施例 Then, in S14, using the particle size information of each particle calculated from the uncharged microbubbles, the charged microbubbles, and the solid particles in S13, the measuring device 7 calculates the particle size dispersed in the liquid to be tested. The particle size distribution Dbo of the uncharged microbubbles, the particle size distribution Dbc of the charged microbubbles, and the particle size distribution Dsolid of the solid particles. Second specific embodiment

然後，參照圖式詳細說明應用本發明第二具體實施例之微氣泡分散液測量方法之該測量系統1A之配置。Then, the configuration of the measurement system 1A to which the method for measuring microbubble dispersion liquid according to the second embodiment of the present invention is applied will be described in detail with reference to the drawings.

圖7為根據此具體實施例之該測量系統1A之部分配置之示意圖。該測量系統1A係根據第一具體實施例進一步對該測量系統1提供一電泳裝置9。請注意圖7中該測量系統1A之組件中，一可變磁場施加裝置5及一測量裝置7皆未顯示。以下，與第一具體實施例之該測量系統1相同之配置會省略其圖示及詳細說明。FIG. 7 is a schematic diagram of a partial configuration of the measurement system 1A according to this embodiment. The measurement system 1A further provides the measurement system 1 with an electrophoretic device 9 according to the first embodiment. Please note that among the components of the measuring system 1A in FIG. 7 , neither a variable magnetic field applying device 5 nor a measuring device 7 is shown. Hereinafter, the same configuration as the measurement system 1 of the first embodiment will be omitted from illustration and detailed description.

電泳裝置9包括：一支架91，其水平地承載一微毛細管2；一對電極支撐部件92, 93，設在該微毛細管2兩端側面；及一DC電源供應器(未顯示)。該支架91為柱狀。支架91之上表面911設有支撐溝槽，其沿該微毛細管2延伸方向延伸且剖面呈弧形。該微毛細管2被設置於裝置9沿著支撐溝槽。此外，支架91在其大致中央處(即在一雷射光L之一照射區域A附近)設有平面圖呈圓形之切口912。The electrophoresis device 9 includes: a bracket 91, which horizontally supports a microcapillary 2; a pair of electrode support members 92, 93, which are arranged on the two ends of the microcapillary 2; and a DC power supply (not shown). The bracket 91 is columnar. The upper surface 911 of the bracket 91 is provided with a supporting groove, which extends along the extending direction of the microcapillary 2 and has an arc-shaped cross section. The microcapillary 2 is arranged in the device 9 along the support groove. In addition, the bracket 91 is provided with a circular cutout 912 in a plan view at its approximate center (ie near an irradiation area A of a laser light L).

電極支撐部件92, 93之每一面(面對該微毛細管2之表面)設置有針狀之電極92a, 93a。此等電極92a, 93a分別連接到DC電源之正極及負極。此外，此等電極支撐部件92, 93被配置成使得該部件92, 93能夠沿著該微毛細管2之延伸方向彼此靠近或彼此遠離。若電極支撐部件92, 93彼此靠近，則電極92a, 93a各自被***到該微毛細管2之導管2a之各端中。Each side of the electrode supporting members 92, 93 (the surface facing the microcapillary 2) is provided with needle-shaped electrodes 92a, 93a. These electrodes 92a, 93a are respectively connected to the positive and negative poles of the DC power supply. In addition, these electrode supporting members 92, 93 are configured such that the members 92, 93 can approach each other or move away from each other along the extending direction of the microcapillary 2. If the electrode support members 92, 93 are close to each other, the electrodes 92a, 93a are each inserted into the respective ends of the conduit 2a of the microcapillary 2.

圖8為使用此本具體實施例之該測量系統1A用於測量該待測液體特性之測量方法之具體步驟之流程圖。在圖8之流程圖中，由於S11-S12、S14-S16及S18-S19之各處理步驟與圖4流程圖中之S1-S8相同，故不再贅述。FIG. 8 is a flow chart of the specific steps of the measurement method for measuring the property of the liquid to be measured using the measurement system 1A of this specific embodiment. In the flow chart of FIG. 8 , since the processing steps of S11-S12, S14-S16 and S18-S19 are the same as those of S1-S8 in the flow chart of FIG. 4 , they are not repeated here.

在S12中該待測液體被盛裝在該微毛細管2後，操作者在S13中將電極支撐部件92, 93彼此靠近以將電極92a, 93a***該微毛細管2之導管2a之兩端。After the liquid to be tested is contained in the microcapillary 2 in S12, the operator moves the electrode support members 92, 93 close to each other in S13 to insert the electrodes 92a, 93a into the two ends of the conduit 2a of the microcapillary 2.

在S16中用一數位顯微鏡6拍攝該照射區域A之動態影像後，在S17中該操作者打開連接到電極92a, 93a之電源一段預定時間以對位於電極92a, 93a間之該照射區域A內之該待測液體供應電場一段預定時間。After taking a dynamic image of the irradiation area A with a digital microscope 6 in S16, the operator turns on the power supply connected to the electrodes 92a, 93a for a predetermined period of time in S17 so as to place an image in the irradiation area A between the electrodes 92a, 93a. The liquid to be tested is supplied with an electric field for a predetermined period of time.

在S19中用該數位顯微鏡6完成動態影像拍攝且完成用一雷射裝置3發射該雷射光L後，在S20中該操作者操作該測量裝置7用以根據該數位顯微鏡6獲得之該微粒子動態影像資料來計算該微粒子之濃度及粒徑分佈。In S19, the digital microscope 6 is used to capture the dynamic image and the laser device 3 is used to emit the laser light L. In S20, the operator operates the measuring device 7 to obtain the particle dynamics according to the digital microscope 6. The image data is used to calculate the concentration and particle size distribution of the microparticles.

此方法中，根據該數位顯微鏡6所獲得之動態影像資料用以區分該微粒子並將其區別為該固體顆粒與微氣泡以及用以計算此等固體顆粒與微氣泡之該粒數濃度及該粒徑分佈之步驟與圖5所示者相同，因此將省略對其詳細描述。In this method, the dynamic image data obtained by the digital microscope 6 is used to distinguish the microparticles and distinguish them into the solid particles and microbubbles and to calculate the particle number concentration and the particle number concentration of these solid particles and microbubbles. The procedure for diameter distribution is the same as that shown in FIG. 5, and thus a detailed description thereof will be omitted.

另外，根據本具體實施例之測量方法，藉由施加電場一預定時間，在S17中施加一電場時獲得一動態影像，該動態影像係對該微粒子施加電場時之電泳軌跡。因此，在S20中，微粒子被識別為固體顆粒及微氣泡，同時藉由根據施加電場時之動態影像分析該待測液體中所含之該固體顆粒之移動速度來測量此等固體顆粒之電特性。 第三具體實施例 In addition, according to the measurement method of this specific embodiment, by applying the electric field for a predetermined time, a dynamic image is obtained when an electric field is applied in S17, and the dynamic image is the electrophoretic trace when the electric field is applied to the microparticle. Therefore, in S20, the microparticles are identified as solid particles and microbubbles, and at the same time, the electrical properties of these solid particles are measured by analyzing the moving speed of the solid particles contained in the liquid to be measured according to the dynamic image when an electric field is applied. . Third specific embodiment

然後，將結合圖式詳細描述一測量系統1B之配置，本發明第三具體實施例之微氣泡分散液之測量方法被施用於該測量系統1B。Then, the configuration of a measurement system 1B to which the method for measuring a microbubble dispersion according to the third embodiment of the present invention is applied will be described in detail with reference to the drawings.

圖9為根據本具體實施例之該測量系統1B之部分配置之示意圖。該測量系統1B被配置成有電泳裝置9B進一步設置於第一具體實施例之該測量系統1。請注意圖9中，在該測量系統1B之組件中未顯示一可變磁場施加裝置5及一測量裝置7。以下將省略與第一具體實施例測量之該測量系統1相同配置之圖示及詳細說明。FIG. 9 is a schematic diagram of a partial configuration of the measurement system 1B according to this embodiment. The measurement system 1B is configured such that an electrophoretic device 9B is further provided in the measurement system 1 of the first embodiment. Please note that in FIG. 9, a variable magnetic field applying device 5 and a measuring device 7 are not shown among the components of the measuring system 1B. The illustration and detailed description of the same configuration as the measurement system 1 measured in the first embodiment will be omitted below.

電泳裝置9B包括：一對電極支撐蓋95, 96，設於一微毛細管2兩端側之；一腳架(stand)(未顯示)，用以垂直承載該微毛細管2；及一電源供應器(未顯示)。The electrophoresis device 9B includes: a pair of electrode support covers 95, 96, which are located at both ends of a microcapillary 2; a stand (stand) (not shown), used to vertically carry the microcapillary 2; and a power supply (not shown).

電極支撐蓋95, 96中分別設有針狀之電極95a, 96a。因此，若將此等電極支撐蓋95, 96安裝到該微毛細管2之兩端，則電極95a, 96a各自都被***到該微毛細管2之導管2a之各端中。Needle-shaped electrodes 95a, 96a are respectively provided in the electrode support covers 95, 96. Therefore, if these electrode supporting caps 95, 96 are mounted to both ends of the microcapillary 2, the electrodes 95a, 96a are each inserted into each end of the conduit 2a of the microcapillary 2.

使用上述該測量系統1B來測量一待測液體特性之測量方法之具體步驟與圖8流程圖中之步驟相同，在此省略詳細說明。The specific steps of the measuring method for measuring the properties of a liquid to be tested using the above-mentioned measuring system 1B are the same as those in the flow chart of FIG. 8 , and detailed descriptions are omitted here.

儘管以上描述本發明部分具體實施例，但本發明不限於此等具體實施例。該具體實施例之配置細節可在本發明標的範圍內根據需要進行改變。Although some specific embodiments of the present invention have been described above, the present invention is not limited to these specific embodiments. The configuration details of this embodiment can be changed as desired within the scope of the present invention.

例如，在第一具體實施例中，根據施加一可變磁場前後之動態影像(參見圖15之S11)將該微粒子識別為不帶電微氣泡、帶電微氣泡及固體顆粒後，藉由分析施加該可變磁場前微粒子布朗運動軌跡來計算各微粒子之粒徑，但計算微粒子中該固體顆粒粒徑之方法不限於此。For example, in the first embodiment, according to the dynamic images before and after applying a variable magnetic field (see S11 of FIG. 15 ), the microparticles are identified as uncharged microbubbles, charged microbubbles, and solid particles, and the microparticles are then analyzed by applying the variable magnetic field. The particle diameter of each particle is calculated by changing the Brownian motion trajectory of the particle before the magnetic field, but the method of calculating the particle size of the solid particle in the particle is not limited to this.

在施加可變磁場時固體顆粒旋轉次數與散射光亮度變化程度相關。再對固體顆粒施加可變磁場時該固體顆粒之旋轉次數也與該固體顆粒之粒徑及介質黏度有關。因此，可根據散射光亮度之變化程度來計算該固體顆粒之粒徑，藉由使用散射光亮度之變化程度與該固體顆粒粒徑及介質黏度之相關性。The number of rotations of solid particles is related to the degree of change in the brightness of scattered light when a variable magnetic field is applied. When a variable magnetic field is applied to the solid particle, the number of rotations of the solid particle is also related to the particle size of the solid particle and the viscosity of the medium. Therefore, the particle size of the solid particle can be calculated according to the change degree of the scattered light brightness, by using the correlation between the change degree of the scattered light brightness and the particle size of the solid particle and the viscosity of the medium.

更具體地，圖10所示之計算固體顆粒粒徑之圖例係根據預先進行之測試作成，該測試係用以測量以下之相關性：在施加可變磁場前後散射光亮度之變化程度，及在不同黏度係數之各種介質中之固體顆粒之粒徑。在施加該可變磁場前後根據該微粒子軌跡動態影像用該測量裝置 7來計算被識別為固體顆粒之微粒子之散射光亮度之變化程度。根據散射光之亮度變化及操作者輸入之介質黏度係數，可在圖例中找到用於計算粒徑之該粒徑，藉此可計算出該固體顆粒之粒徑。More specifically, the graph of calculated solid particle sizes shown in Figure 10 was prepared from previously performed tests to measure the correlation between the degree of change in scattered light brightness before and after application of a variable magnetic field, and in The particle size of solid particles in various media with different viscosity coefficients. Before and after applying the variable magnetic field, use the measurement device 7 to calculate the change degree of the scattered light brightness of the microparticles identified as solid particles according to the dynamic image of the microparticle trajectory. According to the brightness change of the scattered light and the viscosity coefficient of the medium input by the operator, the particle size used to calculate the particle size can be found in the legend, so that the particle size of the solid particle can be calculated.

1, 1A, 1B:測量系統 2:微毛細管(盛裝裝置) 2a:導管 21:導軌板 22:玻璃板 23:聚合物平行線 3:雷射裝置(光源) 4a:不帶電微氣泡 4b:帶電微氣泡 4c, 4d:固體顆粒 5:可變磁場施加裝置 51, 51A:線圈墊 511, 512:圓形線圈 52:主體 6:數位顯微鏡(光檢測裝置) 7:測量裝置 8:過濾裝置 9:電泳裝置 91:支架 911:支架上表面 912:切口 92, 93:電極支撐部件 92a, 93a:電極 95, 96:電極支撐蓋 95a, 96a:電極 L:雷射光 S:散射光 A:照射區域 S1, S2, S3, S4, S5, S6, S7, S8:步驟 S11, S12, S13, S14, S15, S16, S17, S18, S19, S20:步驟 1, 1A, 1B: Measuring system 2: Micro capillary (containment device) 2a: Conduit 21: rail plate 22: glass plate 23: Polymer parallel lines 3: Laser device (light source) 4a: Uncharged microbubbles 4b: Charged microbubbles 4c, 4d: solid particles 5: Variable magnetic field application device 51, 51A: coil pad 511, 512: round coil 52: subject 6: Digital microscope (light detection device) 7: Measuring device 8: Filtration device 9: Electrophoresis device 91: Bracket 911: The upper surface of the bracket 912: incision 92, 93: Electrode support parts 92a, 93a: electrodes 95, 96: Electrode support cover 95a, 96a: electrodes L: laser light S: scattered light A: Irradiation area S1, S2, S3, S4, S5, S6, S7, S8: steps S11, S12, S13, S14, S15, S16, S17, S18, S19, S20: Steps

圖1為本發明第一具體實施例之微氣泡分散液測量系統配置示意圖。 圖2A為一盛裝裝置另一例之透視圖。 圖2B為一盛裝裝置又一例之透視圖。 圖2C為一盛裝裝置其他例之透視圖。 圖3為雙線圈型線圈墊之透視圖。 圖4為用於測量一微氣泡分散液特性之測量方法之具體步驟流程圖。 圖5為使用一測量裝置用於計算微粒子濃度及粒徑分佈之步驟流程圖。 圖6為用數位顯微鏡檢測雷射光之散射光所獲得之微粒子影像之一例示圖。 圖7為本發明第二具體實施例之測量系統之局部配置示意圖。 圖8為用於測量一微氣泡分散液特性之測量方法之具體步驟流程圖。 圖9為本發明第三具體實施例之測量系統之局部配置示意圖。 圖10為用於計算固體顆粒粒徑之例示圖解。 FIG. 1 is a schematic configuration diagram of a microbubble dispersion measurement system according to a first embodiment of the present invention. Figure 2A is a perspective view of another example of a container. Fig. 2B is a perspective view of another example of a container. Fig. 2C is a perspective view of another example of a container. Fig. 3 is a perspective view of a double coil type coil pad. FIG. 4 is a flow chart of specific steps of a measurement method for measuring the properties of a microbubble dispersion. Fig. 5 is a flow chart of steps for calculating particle concentration and particle size distribution using a measuring device. FIG. 6 is an illustration of an image of microparticles obtained by detecting scattered light of laser light with a digital microscope. FIG. 7 is a schematic diagram of a partial configuration of a measurement system according to a second embodiment of the present invention. FIG. 8 is a flow chart of specific steps of a measurement method for measuring the properties of a microbubble dispersion. FIG. 9 is a schematic diagram of a partial configuration of a measurement system according to a third embodiment of the present invention. Figure 10 is an exemplary diagram for calculating the particle size of solid particles.

1:測量系統 1: Measuring system

2:微毛細管(盛裝裝置) 2: Micro capillary (containment device)

3:雷射裝置(光源) 3: Laser device (light source)

5:可變磁場施加裝置 5: Variable magnetic field application device

51:線圈墊 51: coil pad

52:主體 52: subject

6:數位顯微鏡(光檢測裝置) 6: Digital microscope (light detection device)

7:測量裝置 7: Measuring device

8:過濾裝置 8: Filtration device

L:雷射光 L: laser light

S:散射光 S: scattered light

A:照射區域 A: Irradiation area

Claims (15)

一種用於測量一待測液體特性之方法，該待測液體係一微氣泡分散液，該方法包括： 以一照明光照射被盛裝在一盛裝裝置之該待測液體； 在該照明光照射區域內對該待測液體施加一時變磁場； 使用一光檢測裝置發射該照明光來檢測由該待測液體中所含複數個微粒子產生之一散射光；及 根據該光檢測裝置檢測到之該散射光亮度將該等微粒子區分為微氣泡及固體顆粒。 A method for measuring the properties of a liquid to be tested, the liquid to be tested is a microbubble dispersion, the method comprising: irradiating the liquid to be tested contained in a containing device with an illuminating light; applying a time-varying magnetic field to the liquid to be tested in the area irradiated by the illumination light; using a light detection device to emit the illumination light to detect a scattered light generated by the plurality of microparticles contained in the liquid to be tested; and According to the brightness of the scattered light detected by the photodetection device, the microparticles are classified into microbubbles and solid particles. 如請求項1所述之方法，其中該待測液體含有粒徑範圍2 nm至  2000 nm之微氣泡。The method as described in claim 1, wherein the liquid to be tested contains microbubbles with a particle size ranging from 2 nm to 2000 nm. 如請求項1或2所述之方法，其中使用該光檢測裝置所獲得之該等微粒子的一影像與一預定閾值比較其亮度，以將該等微粒子識別為固體顆粒及微氣泡。The method as claimed in claim 1 or 2, wherein the brightness of an image of the microparticles obtained by the photodetection device is compared with a predetermined threshold to identify the microparticles as solid particles and microbubbles. 如請求項1或2所述之方法，其中在檢測該散射光步驟中，在施加一可變磁場之前後，以該光檢測裝置測得之該散射光來獲得該等微粒子因布朗運動所致軌跡之一影像，且 其中在以該光檢測裝置獲得之該影像中，根據在對該待測液體施加該可變磁場時該等微粒子亮度是否增加而將該等微粒子識別為固體顆粒及微氣泡。 The method as described in claim 1 or 2, wherein in the step of detecting the scattered light, before and after applying a variable magnetic field, the scattered light measured by the light detection device is used to obtain the particles due to Brownian motion an image of one of the trajectories, and Wherein, in the image obtained by the light detection device, the microparticles are identified as solid particles and microbubbles according to whether the brightness of the microparticles increases when the variable magnetic field is applied to the liquid to be tested. 如請求項4所述之方法，其中以該光檢測裝置所獲得之該影像中，將對該待測液體施加該可變磁場時，亮度增加之該等微粒子識別為固體顆粒，而將消失之該等微粒子識別為帶電微氣泡，且將亮度未增加之該等微粒子識別為不帶電之微氣泡。The method as described in claim 4, wherein in the image obtained by the photodetection device, when the variable magnetic field is applied to the liquid to be tested, the microparticles whose brightness increases are recognized as solid particles, and will disappear The fine particles were identified as charged microbubbles, and the fine particles whose luminance was not increased were identified as uncharged microbubbles. 如先前請求項中任一項所述之方法，進一步包括測量固體顆粒，其中該待測液體中所含固體顆粒之濃度或粒徑分佈係根據該光檢測裝置所獲得之一影像來計算。The method according to any one of the preceding claims, further comprising measuring solid particles, wherein the concentration or particle size distribution of solid particles contained in the liquid to be measured is calculated based on an image obtained by the light detection device. 如先前請求項中任一項所述之方法，進一步包括測量不帶電微氣泡，其中該待測液體中所含不帶電微氣泡之濃度或粒徑分佈係根據該光檢測裝置所獲得之一影像來計算。The method according to any one of the preceding claims, further comprising measuring uncharged microbubbles, wherein the concentration or particle size distribution of uncharged microbubbles contained in the liquid to be tested is based on an image obtained by the photodetection device to calculate. 如請求項4-7中任一項所述之方法，進一步包括測量帶電微氣泡，其中在檢測散射光之步驟中，獲得以下二者：對該液體施加該可變磁場前該微粒子之布朗運動軌跡之施用前影像；以及對該液體施加可變磁場時該等微粒子之布朗運動軌跡之施用過程影像，且 該待測液體中所含帶電微氣泡之濃度或粒徑分佈係根據該等影像來計算。 The method according to any one of claims 4-7, further comprising measuring charged microbubbles, wherein in the step of detecting scattered light, both of the following are obtained: Brownian motion of the microparticles before the variable magnetic field is applied to the liquid pre-application images of the trajectories; and in-application images of the Brownian motion trajectories of the microparticles when a variable magnetic field is applied to the liquid, and The concentration or particle size distribution of charged microbubbles contained in the liquid to be tested is calculated based on the images. 如請求項8所述之方法，進一步包括進行該待測液體之第一次篩檢，其中將該待測液體盛裝在該盛裝裝置前，該待測液體已通過一帶正電之過濾器。The method according to claim 8, further comprising performing a first screening of the liquid to be tested, wherein the liquid to be tested is contained before the containing device, and the liquid to be tested has passed through a positively charged filter. 如先前請求項中任一項所述之方法， 其中對該液體施加該可變磁場時，根據散射光亮度變化程度，使用該光檢測裝置所獲得該微粒子的影像來計算該固體顆粒之粒徑。The method according to any one of the preceding claims, wherein when the variable magnetic field is applied to the liquid, the particle size of the solid particle is calculated by using the image of the particle obtained by the photodetection device according to the degree of change in brightness of scattered light . 如先前請求項中任一項所述之方法， 其中進一步包括對該待測液體施加一電場，係在該照明光照射區域內對該待測液體施加該電場，其中在檢測散射光之步驟中，對該待測液體施用該電場時獲得該微粒子電泳軌跡之一施用過程影像，且 該待測液體中所含之固體顆粒特性係根據該施用過程影像來測量。 The method according to any one of the preceding claims, further comprising applying an electric field to the liquid to be tested, applying the electric field to the liquid to be tested within the illumination light irradiation area, wherein in the step of detecting scattered light , when the electric field is applied to the liquid to be tested, an application process image of one of the microparticle electrophoretic trajectories is obtained, and The characteristics of the solid particles contained in the liquid to be tested are measured according to the image of the application process. 如先前請求項中任一項所述之方法，進一步包括進行第二次篩檢，其中在對該待測液體施加該可變磁場前，對該液體施加一靜磁場，藉由該靜磁場將至少將一部分該固體顆粒移出該照射區域。The method according to any one of the preceding claims, further comprising performing a second screening, wherein before applying the variable magnetic field to the liquid to be tested, applying a static magnetic field to the liquid, by which the static magnetic field will At least a portion of the solid particles are removed from the irradiated area. 如請求項12所述之方法， 進一步包括測量順磁性物質之數量，其中順磁性顆粒物數量被測定，在對該待測液體施加該靜磁場進行第二次篩檢之步驟中收集該固體顆粒。The method as claimed in claim 12, further comprising measuring the quantity of paramagnetic substances, wherein the quantity of paramagnetic particles is determined, and the solid particles are collected in the second screening step of applying the static magnetic field to the liquid to be tested. 如先前請求項中任一項所述之方法，其中該照明光之光源係一雷射裝置，而該雷射裝置可利用範圍在300 nm至700 nm內之二或多個波長值來切換雷射光(L)波長。A method as claimed in any one of the preceding claims, wherein the source of illumination light is a laser device capable of switching lasers using two or more wavelength values in the range of 300 nm to 700 nm. The emitted light (L) wavelength. 一種用於測量一待測液體特性之測量系統，該待測液體為一微氣泡分散液，該系統包括： 一盛裝裝置，其用於盛裝該待測液體； 一光源，其以一照明光照射盛裝於該盛裝裝置之該待測液體； 一可變磁場施加裝置，其在有該照明光之一照射區域內對該待測液體施加一時變磁場； 一光檢測裝置，其藉由該照明光照射來檢測該待測液體中所含微粒子產生之一散射光；及 一測量裝置，其測量該待測液體特性，根據以該光檢測裝置測得之該散射光亮度將該微粒子區分為微氣泡及固體顆粒。 A measurement system for measuring the properties of a liquid to be tested, the liquid to be tested is a microbubble dispersion, the system comprising: A holding device, which is used to hold the liquid to be tested; a light source, which irradiates the liquid to be tested contained in the containing device with an illuminating light; A variable magnetic field applying device, which applies a time-varying magnetic field to the liquid to be tested in an irradiation area with the illumination light; A light detection device, which detects a scattered light generated by the microparticles contained in the liquid to be tested by irradiation of the illumination light; and A measuring device, which measures the properties of the liquid to be tested, and distinguishes the microparticles into microbubbles and solid particles according to the brightness of the scattered light measured by the light detection device.
TW110119199A 2021-05-27 2021-05-27 Measuring method and measuring system for microbubble dispersion liquid TWI807329B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
TW110119199A TWI807329B (en) 2021-05-27 2021-05-27 Measuring method and measuring system for microbubble dispersion liquid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
TW110119199A TWI807329B (en) 2021-05-27 2021-05-27 Measuring method and measuring system for microbubble dispersion liquid

Publications (2)

Publication Number Publication Date
TW202246755A true TW202246755A (en) 2022-12-01
TWI807329B TWI807329B (en) 2023-07-01

Family

ID=85793775

Family Applications (1)

Application Number Title Priority Date Filing Date
TW110119199A TWI807329B (en) 2021-05-27 2021-05-27 Measuring method and measuring system for microbubble dispersion liquid

Country Status (1)

Country Link
TW (1) TWI807329B (en)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4627726A (en) * 1985-06-17 1986-12-09 The Johns Hopkins University Method and apparatus using laser radiation for generating and measuring gas bubbles
JP4668354B1 (en) * 2010-07-20 2011-04-13 トリオ・セラミックス株式会社 Transparent tube bubble detection device and bubble detection method
EP3458832B1 (en) * 2016-05-20 2021-10-06 Particle Measuring Systems Inc. Automatic power control liquid particle counter with flow and bubble detection systems
EP3662268B1 (en) * 2017-08-01 2021-03-24 Roche Diagnostics GmbH Method of monitoring an operation of detection of an analyte in a liquid sample

Also Published As

Publication number Publication date
TWI807329B (en) 2023-07-01

Similar Documents

Publication Publication Date Title
KR101884108B1 (en) Particle tracking analysis method using scattered light(pta) and device for detecting and identifying particles of a nanometric order of magnitude in liquids of all types
JP6936219B2 (en) Cuvette carrier
JP6726675B2 (en) Particle analyzer and particle analysis method
JP4868190B2 (en) Nano particle measuring device
CN105531578A (en) Biosensor based on measurements of the clustering dynamics of magnetic particles
US20190234853A1 (en) Holographic characterization using hu moments
EP3553512B1 (en) Particle analyzing device and particle analysis method
JP7334957B2 (en) Measurement method and measurement system for microbubble dispersion
WO2008010267A1 (en) Method and apparatus for evaluating strength of dielectrophoresis of fine particles
TWI807329B (en) Measuring method and measuring system for microbubble dispersion liquid
Schaertl et al. Dynamics of colloidal hard spheres in thin aqueous suspension layers—Particle tracking by digital image processing and brownian dynamics computer simulations
WO2022234680A1 (en) Method and system for measuring fine bubble dispersion liquid
JP6727062B2 (en) Detection method and detection device
JP2010101705A (en) Instrument for measuring physical properties of particles
WO2016208724A1 (en) Dispersoid analysis method and analysis device
JP6782971B2 (en) Magnetic field generator, measurement cell, analyzer, and particle separator
JP2014518389A (en) Detection of clusters of magnetic particles
JP2015034747A (en) Shape estimation device and shape estimation method of nonspherical micro objects
RU2006112437A (en) METHOD FOR PRODUCING AN IMPROVED RESOLUTION ABILITY
Iiguni et al. Simultaneous measurement of the migration velocity and adsorption force of micro-particles using an electromagnetophoretic force under a high magnetic field
JP4165674B2 (en) Electrophoretic analysis method and apparatus for fine particles in liquid
Nobbs et al. The design, construction and commissioning of a low-cost optical particle size analyser specifically for measurement of settling velocities and size of flocs
CN1934436A (en) Improved detection device
KR20210107827A (en) Improved method for determining the size of nanoparticles in colloids
JP2009155155A (en) Methods for manufacturing and evaluating metal oxide particulate and the metal oxide particulate