TW202122793A - Wearable measurement device and method of measuring a biological target using the same - Google Patents
Wearable measurement device and method of measuring a biological target using the same Download PDFInfo
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Abstract
Description
本發明實施例係有關於一種量測裝置及生物標的物的量測方法,特別係有關於一種穿戴式量測裝置及其生物標的物的量測方法。The embodiment of the present invention relates to a measurement device and a method for measuring a biological target, and particularly relates to a wearable measurement device and a method for measuring a biological target.
穿戴式及連續式量測裝置已是目前量測裝置追求的目標,但現有的穿戴式及連續式量測裝置中,無論是光學或電化學的量測,都是透過接觸皮膚、微小化針頭、皮下植入等等的接觸式取樣及量測方法,在盡量減少使用者不適感的情況下,進行取樣及量測。而雖然這些方法可以提升使用者的舒適度,但還是無法完全解決使用者在活動時的不適感。Wearable and continuous measurement devices have been the goal pursued by current measurement devices. However, in the existing wearable and continuous measurement devices, whether it is optical or electrochemical measurement, it is through contact with the skin and miniaturized needles. Contact sampling and measurement methods such as subcutaneous implantation, etc., take sampling and measurement while minimizing user discomfort. Although these methods can improve the comfort of the user, they still cannot completely solve the discomfort of the user during activities.
因此,目前業界極需一種新穎的穿戴式量測裝置,以解決使用者在活動時,因為量測裝置接觸皮膚所產生的不適感。Therefore, there is a great need for a novel wearable measurement device in the industry to solve the discomfort caused by the user when the measurement device touches the skin during activities.
本發明實施例的穿戴式量測裝置係透過設置移液元件作為感測電極與皮膚之間的媒介,來避免感測電極直接接觸到皮膚所產生的不適感。The wearable measurement device of the embodiment of the present invention avoids the discomfort caused by the direct contact of the sensing electrode with the skin by arranging the pipetting element as a medium between the sensing electrode and the skin.
根據本發明的一些實施例,提供一種穿戴式量測裝置。此穿戴式量測裝置包含穿戴元件、移液元件、生物感測電極、流量感測電極和儀表。前述穿戴元件具有開口。前述移液元件設置於穿戴元件內並具有透過所述開口暴露於外部的進樣部分。前述生物感測電極以及流量感測電極分別連接至移液元件的第一位置以及第二位置。前述儀表設置於穿戴元件上或內部且電性連接至生物感測電極和流量感測電極。According to some embodiments of the present invention, a wearable measurement device is provided. The wearable measurement device includes a wearable element, a pipetting element, a biological sensing electrode, a flow sensing electrode, and a meter. The aforementioned wearing element has an opening. The aforementioned pipetting element is arranged in the wearing element and has a sample injection part exposed to the outside through the opening. The aforementioned biological sensing electrode and flow sensing electrode are respectively connected to the first position and the second position of the pipetting element. The aforementioned meter is arranged on or inside the wearable element and is electrically connected to the biological sensing electrode and the flow sensing electrode.
根據本發明的一些實施例,提供一種生物標的物的量測方法。此方法包含利用生物感測電極連續量測生物液體的瞬時生物標的物濃度;利用流量感測電極連續量測生物液體的瞬時生物液體量;以及藉由以下公式校正生物標的物濃度: , 其中An代表該瞬時生物標的物濃度,Sn代表該瞬時生物液體量,t為量測時間且c為常數。According to some embodiments of the present invention, a method for measuring a biological target is provided. This method includes continuously measuring the instantaneous biological fluid concentration of the biological fluid with a biological sensing electrode; using the flow sensing electrode to continuously measure the instantaneous biological fluid volume of the biological fluid; and calibrating the biological target concentration by the following formula: , Where An represents the instantaneous biological target concentration, Sn represents the instantaneous biological fluid volume, t is the measurement time and c is a constant.
以下公開許多不同的實施方法或是例子來實行本發明實施例之不同特徵,以下描述具體的元件及其排列的實施例以闡述本發明實施例。當然這些實施例僅用以例示,且不該以此限定本發明實施例的範圍。例如,在說明書中提到第一特徵形成於第二特徵之上,其包括第一特徵與第二特徵是直接接觸的實施例,另外也包括於第一特徵與第二特徵之間另外有其他特徵的實施例,亦即,第一特徵與第二特徵並非直接接觸。此外,在不同實施例中可能使用重複的標號或標示,這些重複僅為了簡單清楚地敘述本發明實施例,不代表所討論的不同實施例及/或結構之間有特定的關係。Many different implementation methods or examples are disclosed below to implement different features of the embodiments of the present invention. The following describes specific elements and their arrangement embodiments to illustrate the embodiments of the present invention. Of course, these embodiments are only for illustration, and should not be used to limit the scope of the embodiments of the present invention. For example, in the specification, it is mentioned that the first feature is formed on the second feature, which includes the embodiment in which the first feature and the second feature are in direct contact, and it also includes other embodiments between the first feature and the second feature. The embodiment of the feature, that is, the first feature and the second feature are not in direct contact. In addition, repeated reference numerals or labels may be used in different embodiments. These repetitions are only used to describe the embodiments of the present invention simply and clearly, and do not represent a specific relationship between the different embodiments and/or structures discussed.
此外,其中可能用到與空間相對用語,例如「在…下方」、「下方」、「較低的」、「上方」、「較高的」及類似的用語,這些空間相對用語係為了便於描述圖示中一個(些)元件或特徵與另一個(些)元件或特徵之間的關係,這些空間相對用語包括使用中或操作中的裝置之不同方位,以及圖式中所描述的方位。當裝置被轉向不同方位時(旋轉90度或其他方位),則其中所使用的空間相對形容詞也將依轉向後的方位來解釋。In addition, terms relative to space may be used, such as "below", "below", "lower", "above", "higher" and similar terms. These spatial relative terms are used for ease of description The relationship between one element or feature(s) and another element(s) or feature in the illustration. These spatial relative terms include the different orientations of the device in use or operation, as well as the orientation described in the drawings. When the device is turned in different directions (rotated by 90 degrees or other directions), the spatially relative adjectives used therein will also be interpreted according to the turned position.
在此,「約」、「大約」、「大抵」之用語通常表示在一給定值或範圍的20%之內,較佳是10%之內,且更佳是5%之內,或3%之內,或2%之內,或1%之內,或0.5%之內。應注意的是,說明書中所提供的數量為大約的數量,亦即在沒有特定說明「約」、「大約」、「大抵」的情況下,仍可隱含「約」、「大約」、「大抵」之含義。Here, the terms "about", "approximately", and "approximately" usually mean within 20% of a given value or range, preferably within 10%, and more preferably within 5%, or 3 Within %, or within 2%, or within 1%, or within 0.5%. It should be noted that the quantity provided in the manual is an approximate quantity, that is, without specifying "about", "approximately", "approximately", "about", "approximately" and "approximately" can still be implied. The meaning of "probably".
能理解的是,雖然在此可使用用語「第一」、「第二」、「第三」等來敘述各種元件、組成成分、區域、層、部分及/或區段,這些元件、組成成分、區域、層、部分及/或區段不應被這些用語限定,且這些用語僅是用來區別不同的元件、組成成分、區域、層、部分及/或區段。因此,以下討論的一第一元件、組成成分、區域、層、及/或部分可在不偏離本揭露之教示的情況下被稱為一第二元件、組成成分、區域、層、部分及/或區段。It can be understood that although the terms "first", "second", "third", etc. may be used herein to describe various elements, components, regions, layers, parts, and/or sections, these elements and components , Regions, layers, parts, and/or sections should not be limited by these terms, and these terms are only used to distinguish different elements, components, regions, layers, sections, and/or sections. Therefore, a first element, component, region, layer, and/or portion discussed below may be referred to as a second element, component, region, layer, portion, and/or without departing from the teachings of the present disclosure. Or section.
雖然所述的一些實施例中的步驟以特定順序進行,這些步驟亦可以其他合邏輯的順序進行。在不同實施例中,可替換或省略一些所述的步驟,亦可於本發明實施例所述的步驟之前、之中、及/或之後進行一些其他操作。Although the steps in some of the described embodiments are performed in a specific order, these steps can also be performed in other logical orders. In different embodiments, some of the steps described may be replaced or omitted, and some other operations may also be performed before, during, and/or after the steps described in the embodiments of the present invention.
請參閱第1圖,其繪示根據本發明一實施例的穿戴式量測裝置100的平面圖,穿戴式量測裝置100包含穿戴元件102、移液元件104、生物感測電極106、流量感測電極108和儀表110。Please refer to FIG. 1, which shows a plan view of a
穿戴元件102具有開口。具體而言,穿戴元件102的開口面對使用者的皮膚。在一些實施例中,穿戴元件102包含帽子、頭帶、腕帶、衣服或前述類似元件。舉例而言,開口可位於帽子的帽身、頭帶的內側、衣服的袖口或衣領等等。The wearing
移液元件104可設置於穿戴元件102內,並具有進樣部分1041。進樣部分1041透過穿戴元件102的開口(帽口)暴露於外部以與皮膚接觸。生物液體透進樣部分1041進入移液元件104。移液元件104可具有單層或多層結構,且其形狀可包含,但不限於半圓形、圓形、正方形、長方形、L型、T型以及Y型。移液元件104可為幫浦或吸水性材料。吸水性材料的實例包含但不限於超高吸水性樹脂(SAP)、親水性纖維材料或其組合。由於移液元件104為幫浦或吸水性材料,所以進入移液元件104的生物液體可藉由幫浦的主動吸取或吸水性材料的毛細管作用在移液元件104中移動。在一些實施例中,生物液體包含汗液、血液或其它類似液體。The
生物感測電極106為可用以測量生物液體的生物標的物濃度的電極,其中生物標的物(或可視為生化物質、代謝物等)可包含電解質、葡萄糖、乳酸或前述類似物。流量感測電極108為可用以測量生物液體量的電極。生物感測電極106以及流量感測電極108分別設置於穿戴元件102內且與移液元件104連接。透過與移液元件104連接,生物液體可在生物感測電極106以及流量感測電極108不接觸皮膚的情況下移動至生物感測電極106以及流量感測電極108,進而減少使用者因直接接觸生物感測電極106及/或流量感測電極108產生的不適感。生物感測電極106以及流量感測電極108可連接至移液元件104的任何位置。舉例而言,如第1圖所示,當移液元件104為具有三個端部的Y型形狀時,進樣部分1041可位於移液元件104的一個端部,而生物感測電極106以及流量感測電極108可分別位於移液元件104另外兩個端部附近以與移液元件104連接。於此實施例中,穿戴式量測裝置100中的生物感測電極106與進樣部分1041之間的距離以及流量感測電極108與進樣部分1041之間的距離兩者大致相當,因此生物液體約同時移動至生物感測電極106以及流量感測電極108,但本發明不限於此。The
第2A-2C圖為根據本發明的一些實施例繪示的生物感測電極106的示意圖。生物感測電極106包含導電元件106A。導電元件106A可為電極或導電織帶。電極包含導電材料,而導電織帶係由導電材料(導電織線)和人造纖維或天然纖維編織而成。在一實施例中,導電元件106A可直接接觸移液元件104以與在其中流動的生物液體的生物標的物反應,如第2C圖所示。在此種實施例中,導電元件106A可包含但不限於鈷、鎳、硒、鈷氧化物、鎳氧化物、硒氧化物、銀碳、石墨烯(graphene)碳奈米管(carbon nanotube)或其任意組合或碳奈米管的導電材料,上述材料作為電極或導電織帶內的導電織線。在另一實施例中,生物感測電極106可進一步包含反應部件106B。反應部件106B包含反應物10與固定層106B’。反應部件106B位於導電元件106A與移液元件104之間。反應部件XX可直接接觸移液元件104以與在其中流動的生物液體的生物標的物反應,導電元件106A接著根據反應變化產生電訊號,如第2A、2B圖配置。在此種實施例中,導電元件106A可包含鋁(Al)、鉬(Mo)、鎢(W)、鈦(Ti)、鉭(Ta)、鉑(Pt)、鉿(Hf)、鎳(Ni)、鈷(Co)、鋅(Zb)、石墨或前述類似材料的導電材料,但本發明不限於此。反應物10可包含固定化酵素、酵素、抗體、蛋白質、核酸、化學反應試劑或前述之組合,但本發明不限於此。舉例而言,反應物10可包含葡萄糖氧化酶(glucose oxidase)、葡萄糖脫氫酶(glucose dehydrogenase)、乳酸氧化酶(lactate oxidase)、乳酸脫氫酶(lactate dehydrogenase)、3-羥丁酸脫氫酶(3-hydroxybutyrate dehydrogenase)、尿素酶(urease)、膽固醇酯酵素(cholesterol esterase)、膽固醇氧化酶(cholesterol oxidase)或其任意組合,但本發明不限於此。本發明所屬技術領域中具有通常知識者理解的是,反應物的種類取決於所欲測量的生物標的物的種類。2A-2C are schematic diagrams of the
反應物10可透過各種方式固定於導電元件106A上。在一實施例中,如第2A圖所示,反應部件106B可進一步包含用以固定反應物10的固定層106B’。固定層106B’的材料包含聚乙烯吡咯烷酮(poly vinyl pyrrolidone,PVP)、聚乙二醇(poly ethylene glycol,PEG)、聚乙烯亞胺(poly ethylene imine,PEI)、聚乙烯醇(polyvinyl alcohol,PVA)或羧甲基纖維素(Carboxymethyl cellulose,CMC)。固定層106B’可位於反應物10上。固定層106B’亦可位於反應物10和導電元件106A之間。反應物10亦可位於兩層固定層106B’之間。The
在另一實施例中,舉例而言,當反應物10為固定化酵素時,反應物10可利用1-乙基-3-(3-二甲基胺基丙基)(EDC)/N-羥基琥珀醯亞胺(NHS)、EDC/sulfo-NHS、戊二醛(glutaraldehyde,GA)、Nafion或電化學方法直接固定於導電元件106A而無須固定層,如第2B圖所示。In another embodiment, for example, when the
生物感測電極106可用一般電流電壓、微分脈衝伏安法(differential pulse voltammetry,DPV)或方波伏安法(square wave voltammetry,SWV)等電化學方法來量測生物液體的生物標的物。The
微分脈衝伏安法是在直流電壓上疊加一定幅值(例如10-100 mV)的脈衝電壓進行測定的方法。方波伏安法(SWV)是對電極給予一大振幅微分脈衝之方形波電位,經方波循環週期收集兩次反應電流,分別是順向脈衝(Forward pulse)的末點電流與逆向脈衝(Reverse pulse)之末點電流,兩點相減可得淨電流(Net current),具有放大訊號之效果,與微分脈衝伏安法比較有較佳的靈敏度。此外,方波伏安法主要優點還包含快速掃描,有效掃描速率為方波頻率與電位變化相乘,因此可減少掃描時間以快速完成分析。Differential pulse voltammetry is a method of superimposing a pulse voltage of a certain amplitude (for example, 10-100 mV) on a DC voltage for measurement. Square wave voltammetry (SWV) is to give a square wave potential of a large amplitude differential pulse to the electrode, and collect two reaction currents through the square wave cycle. They are the end current of the forward pulse and the reverse pulse ( The last point current of Reverse pulse), the net current can be obtained by subtracting the two points, which has the effect of amplifying the signal, and has better sensitivity than the differential pulse voltammetry. In addition, the main advantages of square wave voltammetry include fast scanning. The effective scanning rate is the square wave frequency multiplied by the potential change, so the scanning time can be reduced to complete the analysis quickly.
第3圖係利用穿戴式量測裝置100之生物感測電極106以安培法量測生物標的物10分鐘之反應電流訊號變化圖。第3圖係以吸水性材料作為移液元件104、以葡萄糖作為生物標的物,並以在導電元件106A上直接固定葡萄糖氧化酶(等同反應物10)的生物感測電極106作為示例,如第2B圖態樣,來說明根據本發明實施例的穿戴式量測裝置100的可行性。首先將移液元件104的一側作為進樣部分1041,並將移液元件104相對於進樣部分1041的一側與生物感測電極106連接,如第1圖所示。於進樣部分1041分數次加入葡萄糖溶液,藉由移液元件104的吸水力以及移液功能,葡萄糖溶液可在移液元件104中移動並流過生物感測電極106的反應區,最後自生物感測電極106的收集反應電流訊號,其結果如第3圖所示。由第3圖可發現每次加入葡萄糖溶液時,皆可偵測到波峰(如箭頭所示),波峰的電流值能換算為葡萄糖濃度,其表示在加入葡萄糖溶液之後,反應電流會快速上升,之後再回到較高的穩定反應電流。藉由紀錄穩定反應電流值,並搭配以標準品建立之表格,可計算出樣品中葡萄糖濃度。此示例證實生物液體可利用吸水材質的移液功能持續送到生物感測電極106的反應區來進行電化學反應,且本案的穿戴式量測裝置100可連續且多次的量測樣品中的生物標的物濃度。FIG. 3 is a graph showing the change of the response current signal of the biological target object measured by the amperometric method using the
第4A-4F圖係根據本發明的一些實施例繪示流量感測電極108與移液元件104的配置示意圖。上述導電組件可為電極或導電織帶且可細部量測較小的生物液體量變化。電極包含導電材料,而導電織帶係由導電材料和人造纖維或天然纖維編織而成。導電材料的實例可包含但不限於,鋁(Al)、鉬(Mo)、鎢(W)、鈦(Ti)、鉭(Ta)、鉑(Pt)、鉿(Hf)、鎳(Ni)、鈷(Co)、鋅(Zb)、石墨或前述類似材料。4A-4F are schematic diagrams showing the configuration of the
在一實施例中,流量感測電極108可包含兩個以上的導電組件。如第4A圖所示,與移液元件104連接的流量感測電極108可包含第一導電組件108A、第二導電組件108B、第三導電組件108C、第四導電組件108D以及第五導電組件108E,其中第一導電組件108A與進樣部分之間的距離大於第二導電組件108B與進樣部分之間的距離,第二導電組件108B與進樣部分之間的距離大於第三導電組件108C與進樣部分之間的距離,第三導電組件108C與進樣部分之間的距離大於第四導電組件108D與進樣部分之間的距離,且第四導電組件108D與進樣部分之間的距離大於第五導電組件108E與進樣部分之間的距離。各個導電組件具有雙線電極,以量測電位差,各個導電組件之間具有一固定距離。生物液體的移動方向如第4A圖中的箭頭所示,因此,生物液體會在先移動到第五導電組件108E,接著依序移動到第四導電組件108D、第三導電組件108C、第二導電組件108B,最後才移動到第一導電組件108A。於此實施例中,第五導電組件108E會先量測到電流以及電壓的變化,接著第四導電組件108D至第一導電組件108A會依序量測到電流以及電壓的變化。具有上述配置的流量感測電極108可量測大的生物液體變化,並可透過第一導電組件108A至第五導電組件108E分別測得之生物液體量變化獲得精確的生物液體總量或含水量(又稱水分含量,含濕量)。In an embodiment, the
在另一實施例中,如第4B圖所示,與移液元件104連接的流量感測電極108可包含第一導電組件108A(單線電極)以及第二導電組件108B(雙線電極)。生物液體變化的量測精準度可透過增加第一導電組件108A以及第二導電組件108B用以與生物液體接觸的露出感應區的長度來提高。在第4B圖所示之實施例中,生物液體量由箭頭處進入,當生物液體量越來越大時,第二導電組件108B量測到電流以及電壓的變化也會越來越大。當生物液體量大到一定程度時,生物液體會移動到第一導電組件108A,此時第一導電組件108A也開始量測到電流以及電壓的變化。藉由上述配置,流量感測電極108可量測大的生物液體變化,並可透過第一導電組件108A以及第二導電組件108B分別測得之生物液體量變化獲得精確的生物液體總量或含水量。In another embodiment, as shown in FIG. 4B, the
流量感測電極108中的導電組件的配置可視需要地調整。舉例而言,流量感測電極108的導電組件可如第4C圖(互相相對的4條直線電極)所示地幾何配置、如第4D圖(2個趾狀電極互相交錯)以及第4E圖所示地柵極配置或如第4F圖所示地S型交叉配置(空間中重疊但不接觸),但本發明不限於此。量測方式除了擷取電流及/或電壓變化以外,亦可使用例如DVP、SWV等電化學方式進行。The configuration of the conductive components in the
以下進一步詳細描述流量感測電極108的量測原理。第5A圖係根據本發明另一實施例繪示的流量感測電極108的配置示意圖。如第5A圖所示,與移液元件104連接的流量感測電極108包含第一導電組件108A、第二導電組件108B、第三導電組件108C和第四導電組件108D(為簡化說明,第5A圖中僅以兩點表示各導電組件的露出感應區或採用點狀電極)。第5A圖中的箭頭方向表示生物液體的移動方向,第一導電組件108A遠離進樣部分,第三導電組件108C最靠近進樣部分,第二以及第四導電組件108B和108D介於第一導電組件108A和第三導電組件108C之間。The measurement principle of the
第5B圖係具有第5A圖所示地配置的導電組件的流量感測電極108的電訊號變化圖。每三分鐘於進樣部分加入磷酸鹽緩衝溶液(phosphate buffer solution,PBS)。由於第三導電組件108C最靠近進樣部分,所以第三導電組件108C先有回應。如第5B圖所示,當PBS的累積進樣量達50 mL時,第三導電組件108C的電壓最早下降,電壓下降代表量測到PBS的電位。第二以及第四導電組件108B和108D比第三導電組件108C稍微遠離進樣口,因此第二以及第四導電組件108B和108D在第三導電組件108C之後才有回應。如第5B圖所示,接著,當PBS的累積進樣量達100 mL和150 mL時,第二以及第四導電組件108B和108D的電壓在第三導電組件108C之後才下降。接著,由於第一導電組件108A離進樣部分最遠,所以第一導電組件108A最後才會有回應。如第5B圖所示,當PBS的累積進樣量達550 mL時,第一導電組件108A的電壓才下降。因此,可利用電極組件的配置,來計算生物液體的含水量。FIG. 5B is a diagram of electrical signal changes of the
回第1圖,儀表110可設置於穿戴元件102上或內部,並與生物感測電極106以及流量感測電極108電性連接以接收來自生物感測電極106以及流量感測電極108的電訊號。依據生物感測電極106以及流量感測電極108與移液元件104連接的位置,來自生物感測電極106以及流量感測電極108的電訊號可同時或先後到達儀表110。儀表110可包含運算模組以獲得並記錄生物液體的生物標的物濃度、生物液體總量以及經校正的生物標的物濃度。在另一實施例中,儀表110可進一步包含供電模組以向生物感測電極106以及流量感測電極108提供電力。在另一實施例中,儀表110可進一步包含顯示模組以顯示生物液體的生物標的物濃度、生物液體總量及/或經校正的生物標的物濃度。Returning to Figure 1, the
第6圖繪示了根據本發明另一實施例的穿戴式量測裝置200的平面圖。如第6圖所示,移液元件204具有L型形狀,L型移液元件204設置於穿戴元件202內部,且進樣部分2041位於L型移液元件204的一端部。生物感測電極206以及流量感測電極208分別設置於穿戴元件202內並與移液元件204連接。如第6圖所示,生物感測電極206以及流量感測電極208分別連接至移液元件204的第一位置204A以及第二位置204B,其中第二位置204B與進樣部分2041的距離大於第一位置204A與進樣部分2041的距離。。於穿戴式量測裝置200中,由於與流量感測電極208相比,生物感測電極206較相近於進樣部分2041,因此,生物液體會先到達生物感測電極206然後再到達流量感測電極208,生物感測電極206連續量測生物液體的瞬時(real time)生物標的物濃度或即時生物標的物濃度,且利用流量感測電極208連續量測生物液體的瞬時生物液體量,但本發明不限於此。在一實施例中,流量感測電極可比生物感測電極較接近於進樣部分,以使生物液體會先到達流量感測電極然後再到達生物感測電極。穿戴式量測裝置200中的穿戴元件202、儀表210、生物感測電極206、流量感測電極208具有與穿戴式量測裝置100中的穿戴元件102、儀表110、生物感測電極106、流量感測電極108相似的結構,故於此不再贅述。以下以穿戴式量測裝置200作為示例進一步解釋本案的穿戴式量測裝置的具體運作方式。FIG. 6 shows a plan view of a
第7圖係為根據本發明實施例的穿戴式量測裝置200的運作流程圖。如第7圖所示,穿戴式量測裝置200的運作流程圖包含進樣步驟S701、產生第一電訊號的步驟S703、產生第二電訊號的步驟S704、訊號處理步驟S705以及輸出及/或紀錄結果的步驟S707。同時參照第6圖以及第7圖,首先,在進樣步驟S701中,生物液體被吸取並透過進樣部分2041進入L型移液元件204。於第6圖所示之實施例中,生物液體會先移動至生物感測電極206然後再到流量感測電極208。在步驟S703中,生物液體會移動到生物感測電極206,此時生物感測電極206會量測生物液體流過流量感測電極206時的反應電流值,並據以產生第一電訊號。在步驟S704中,生物液體移動會至流量感測電極208,此時流量感測電極208會量測生物液體流過流量感測電極208時的電流變化以及電壓變化,並據以產生第二電訊號。步驟S703以及步驟S704的執行順序無特別限定,其可同時或先後進行。在訊號處理步驟S705中,儀表210接收第一電訊號以及第二電訊號後運算以獲得生物標的物濃度以及生物液體總量,並進一步依據如下所示之校正公式來獲得經校正的生物標的物濃度。校正公式中之An代表生物感測電極206所測到的瞬時生物標的物濃度,Sn代表流量感測電極208所測到的瞬時生物液體量或含水量,t為量測時間且c為常數。將瞬時生物標的物濃度An與瞬時生物液體量Sn相乘之後對時間t作積分,加上常數c後即可獲得經校正的生物標的物濃度或平均監測量。 FIG. 7 is a flowchart of the operation of the
最後,於輸出及/或紀錄結果的步驟S707中輸出及/或紀錄依據第一電訊號以及第二電訊號運算所得之生物液體的生物標的物濃度、生物液體總量及/或經校正的生物標的物濃度,提供持續性的累積量測數據。Finally, in step S707 of outputting and/or recording the result, outputting and/or recording the concentration of the biological target, the total amount of biological fluid and/or the corrected biological fluid calculated based on the first electrical signal and the second electrical signal The concentration of the target substance provides continuous cumulative measurement data.
相較於現有技術的穿戴式量測裝置,本發明實施例所提供的穿戴式量測裝置具有以下一或多個優點,但不限於此: (1) 由於本發明實施例的穿戴式量測裝置是透過將移液元件設置於穿戴元件的開口,所以可避免感測電極直接接觸到皮膚,藉此能避免感測電極接觸至皮膚所產生的不適感。 (2) 由於生物液體可不斷地被移液元件運送至生物感測電極和流量感測電極,所以本發明實施例的穿戴式量測裝置可連續地測量生物液體的生物標的物和生物液體量。 (3) 再者,移液元件、生物感測電極和流量感測電極為拋棄式的模組,僅需更換移液元件、生物感測電極和流量感測電極即可測量不同的生物標的物。 (4) 此外,本發明實施例還透過含水量來校正生物液體的生物標的物的濃度,可更精確地反應使用者的生理狀況。Compared with the wearable measurement device of the prior art, the wearable measurement device provided by the embodiment of the present invention has one or more of the following advantages, but is not limited thereto: (1) Since the wearable measurement device of the embodiment of the present invention arranges the pipetting element in the opening of the wearable element, it can prevent the sensing electrode from directly contacting the skin, thereby preventing the sensing electrode from contacting the skin. Of discomfort. (2) Since the biological fluid can be continuously transported by the pipetting element to the biological sensing electrode and the flow sensing electrode, the wearable measurement device of the embodiment of the present invention can continuously measure the biological target substance and the amount of biological fluid. . (3) In addition, the pipetting element, biosensing electrode and flow sensing electrode are disposable modules. You only need to replace the pipetting element, biological sensing electrode and flow sensing electrode to measure different biological targets. . (4) In addition, the embodiment of the present invention also corrects the concentration of the biological target substance in the biological liquid through the water content, which can more accurately reflect the physiological condition of the user.
雖然本發明的實施例及其優點已揭露如上,但應該瞭解的是,任何所屬技術領域中具有通常知識者,在不脫離本揭露之精神和範圍內,當可作更動、替代與潤飾。此外,本揭露之保護範圍並未侷限於說明書內所述特定實施例中的製程、機器、製造、物質組成、裝置、方法及步驟,任何所屬技術領域中具有通常知識者可從本揭露揭示內容中理解現行或未來所發展出的製程、機器、製造、物質組成、裝置、方法及步驟,只要可以在此處所述實施例中實施大抵相同功能或獲得大抵相同結果皆可根據本揭露使用。因此,本揭露之保護範圍包括上述製程、機器、製造、物質組成、裝置、方法及步驟。另外,每一申請專利範圍構成個別的實施例,且本揭露之保護範圍也包括各個申請專利範圍及實施例的組合。Although the embodiments of the present invention and its advantages have been disclosed as above, it should be understood that any person with ordinary knowledge in the relevant technical field can make changes, substitutions and modifications without departing from the spirit and scope of the present disclosure. In addition, the scope of protection of this disclosure is not limited to the manufacturing process, machinery, manufacturing, material composition, device, method, and steps in the specific embodiments described in the specification. Anyone with ordinary knowledge in the technical field can disclose the content from this disclosure. It is understood that the current or future developed processes, machines, manufacturing, material composition, devices, methods, and steps can be used according to the present disclosure as long as they can implement substantially the same functions or obtain substantially the same results in the embodiments described herein. Therefore, the protection scope of the present disclosure includes the above-mentioned manufacturing processes, machines, manufacturing, material composition, devices, methods, and steps. In addition, each patent application scope constitutes an individual embodiment, and the protection scope of the present disclosure also includes each patent application scope and a combination of embodiments.
10:反應物
100, 200:穿戴式量測裝置
102, 202:穿戴元件
104, 204:移液元件
106, 206:生物感測電極
106A:導電元件
106B:反應部件
106B’:固定層
108, 208:流量感測電極
106A、108A, 108B, 108C, 108D, 108E:導電組件
110, 210:儀表
1041, 2041:進樣部分
S701, S703, S704, S705, S707:步驟10:
以下將配合所附圖式詳述本發明實施例。應注意的是,依據在業界的標準做法,各種特徵並未按照比例繪製且僅用以說明例示。事實上,可能任意地放大或縮小元件的尺寸,以清楚地表現出本發明實施例的特徵。 第1圖係根據本發明一實施例繪示的穿戴式量測裝置的平面圖。 第2A-2C圖係根據本發明一些實施例繪示的生物感測電極的示意圖。 第3圖係利用電化學儀以安培法量測之根據本發明一些實施例的生物感測電極的電訊號變化圖。 第4A-4F圖係根據本發明一些實施例繪示的流量感測電極的配置示意圖。 第5A圖係根據本發明另一實施例繪示的流量感測電極的配置示意圖。 第5B圖係具有第5A圖所示的配置的流量感測電極的電訊號變化圖。 第6圖係根據本發明另一實施例繪示的穿戴式量測裝置的平面圖。 第7圖係為第6圖所示的穿戴式量測裝置的運作流程圖。The embodiments of the present invention will be described in detail below in conjunction with the drawings. It should be noted that, according to standard practices in the industry, various features are not drawn to scale and are only used for illustration and illustration. In fact, it is possible to arbitrarily enlarge or reduce the size of the element to clearly show the characteristics of the embodiment of the present invention. FIG. 1 is a plan view of a wearable measurement device according to an embodiment of the present invention. 2A-2C are schematic diagrams of biosensing electrodes according to some embodiments of the present invention. FIG. 3 is a graph showing changes in electrical signals of the biosensing electrode according to some embodiments of the present invention measured by the amperometric method using an electrochemical instrument. Figures 4A-4F are schematic diagrams showing the configuration of flow sensing electrodes according to some embodiments of the present invention. FIG. 5A is a schematic diagram showing the configuration of flow sensing electrodes according to another embodiment of the present invention. FIG. 5B is a diagram of electrical signal changes of the flow sensing electrode having the configuration shown in FIG. 5A. FIG. 6 is a plan view of a wearable measurement device according to another embodiment of the present invention. Fig. 7 is a flowchart of the operation of the wearable measurement device shown in Fig. 6.
100:穿戴式量測裝置100: Wearable measuring device
102:穿戴元件102: Wearable components
104:移液元件104: Pipetting element
1041:進樣部分1041: Sampling part
106:生物感測電極106: Biological sensing electrode
108:流量感測電極108: Flow sensor electrode
110:儀表110: Meter
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