TW201740808A - Bacillus amyloliquefaciens RTI472 compositions and methods of use for benefiting plant growth and treating plant disease - Google Patents

Bacillus amyloliquefaciens RTI472 compositions and methods of use for benefiting plant growth and treating plant disease Download PDF

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TW201740808A
TW201740808A TW105115451A TW105115451A TW201740808A TW 201740808 A TW201740808 A TW 201740808A TW 105115451 A TW105115451 A TW 105115451A TW 105115451 A TW105115451 A TW 105115451A TW 201740808 A TW201740808 A TW 201740808A
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plant
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phytophthora
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methyl
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賽夫雅 泰歌夫
德 雷李 丹尼爾 凡
梅琳Ａ Ｒ 席琳絲基
李在憲
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富曼西公司
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Abstract

Compositions and methods include a new strain of Bacillus amyloliquefaciens having activity against plant pathogens. The compositions are useful for benefiting plant growth and/or conferring protection against a pathogenic infection when applied to plant foliage, flowers, fruits, bark, roots, seeds, callus tissue, grafts, cuttings, surrounding soil or growth medium, and soil or growth medium concomitant with sowing seed and planting callus tissue, grafts, and cuttings. In one example, the Bacillus amyloliquefaciens RTI472 strain can be delivered to the plant as part of an integrated pest management program, with other microbial or chemical insecticides, fungicides, nematicides, bacteriocides, herbicides, plant extracts, and plant growth regulators.

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解澱粉芽孢桿菌RTI472組合物及其於使植物生長獲益及治療植物疾病之使用方法 Bacillus amyloliquefaciens RTI472 composition and use method thereof for benefiting plant growth and treating plant diseases 相關申請案之交叉參考Cross-reference to related applications

本申請案主張2014年12月29日申請之美國臨時申請案第62/097,207號之權益，該案之揭示內容以全文引用的方式併入本文中。 The present application claims the benefit of U.S. Provisional Application No. 62/097,207, filed on Dec. 29, 2014, the disclosure of which is hereby incorporated by reference.

本發明揭示之標的物係關於包含解澱粉芽孢桿菌RTI472之分離株之組合物，其用於施用於植物葉、植物果實及花、植物種子及根部以及植物周圍土壤，以使植物生長獲益及治療植物疾病。 The subject matter disclosed herein relates to a composition comprising an isolate of Bacillus amyloliquefaciens RTI472 for application to plant leaves, plant fruits and flowers, plant seeds and roots, and soil surrounding plants to benefit plant growth and Treat plant diseases.

已知對植物生長及健康具有有益作用之多種微生物存在於土壤中，特別與植物聯合存活於根區中(植物根圍促生長細菌「PGPR」)，或以內寄生菌形式存在於植物內。其有益促進植物生長特性包括固氮；鐵螯合；磷酸鹽溶解；抑制非有益微生物；抗蟲害；誘導系統抗性(ISR)；系統獲得抗性(SAR)；植物物質分解於土壤中以增加有用土壤有機物；及合成植物激素，諸如吲哚-乙酸(IAA)、乙偶姻及2,3-丁二醇，其刺激植物生長、發育及對環境壓力(諸如乾旱)作出反應。另外，此等微生物可藉由使前驅分子1-胺基環丙烷-1-甲酸酯 (ACC)分解來干擾植物之乙烯壓力反應，藉此刺激植物生長且減緩果實熟化。此等有益微生物可改善土壤品質、植物生長、產量及作物品質。各種微生物展現生物活性以便適用於控制植物疾病。此類生物殺蟲劑(活生物體及由此等生物體天然產生之化合物)可比合成肥料及殺蟲劑更安全且更生物可降解。 A variety of microorganisms known to have beneficial effects on plant growth and health are present in the soil, particularly in combination with plants in the root zone (plant root growth promoting bacteria "PGPR"), or in the form of endoparasites in plants. It is beneficial to promote plant growth characteristics including nitrogen fixation; iron chelation; phosphate dissolution; inhibition of non-beneficial microorganisms; insect resistance; induced systemic resistance (ISR); systemic acquired resistance (SAR); decomposition of plant matter into the soil to increase usefulness Soil organic matter; and synthetic plant hormones such as guanidine-acetic acid (IAA), acetoin and 2,3-butanediol, which stimulate plant growth, development, and respond to environmental stresses such as drought. In addition, these microorganisms can be made by the precursor molecule 1-aminocyclopropane-1-carboxylate. (ACC) decomposes to interfere with the ethylene stress response of the plant, thereby stimulating plant growth and slowing fruit ripening. These beneficial microorganisms can improve soil quality, plant growth, yield and crop quality. Various microorganisms exhibit biological activity for use in controlling plant diseases. Such biocides (live organisms and naturally occurring compounds of such organisms) are safer and more biodegradable than synthetic fertilizers and insecticides.

真菌植物病原體，包括(但不限於)葡萄孢屬(Botrytis spp.)(例如灰葡萄孢菌(Botrytis cinerea))、鐮刀菌屬(Fusarium spp.)(例如尖孢鐮刀菌(F.oxysporum)及禾穀鐮刀菌(F.graminearum))、絲核菌屬(Rhizoctonia spp.)(例如紋枯病菌(R.solani))、稻瘟菌屬(Magnaporthe spp.)、球腔菌屬(Mycosphaerella spp.)、柄鏽菌屬(Puccinia spp.)(例如小麥葉鏽菌(P.recondita))、疫菌屬(Phytopthora spp.)及層鏽菌屬(Phakopsora spp.)(例如豆薯層鏽菌(P.pachyrhizi))為一種類型之植物蟲害，其可在農業及園藝行業中造成嚴重經濟損失。化學試劑可用於控制真菌植物病原體，但使用化學試劑具有如下缺點，包括成本高、缺乏功效、出現真菌抗性菌株及不希望之環境影響。另外，除靶向處理之植物病原體之外，此類化學處理往往不加選擇而會對有益細菌、真菌及節肢動物有不利地影響。第二種類型之植物蟲害為細菌病原體，包括(但不限於)歐文氏菌屬(Erwinia spp.)(諸如菊歐文氏菌(Erwinia chrysanthemi))、泛菌屬(Pantoea spp.)(諸如綠檸檬泛菌(P.citrea))、黃單孢菌屬(Xanthomonas)(例如野油菜黃單孢菌(Xanthomonas campestris))、假單孢菌屬(Pseudomonas spp.)(諸如丁香假單孢菌(P.syringae))及青枯病菌屬(Ralstonia spp.)(諸如番茄青枯病菌(R.soleacearum))，其在農業及園藝行業中造成嚴重經濟損失。類似於病原性真菌，使用化學試劑處理此等細菌病原體具有缺點。病毒及病毒樣生物體包含第三種類型之植物致病劑，其難以控制，但細菌微生物可經由誘導系統抗性(ISR)對其提供植物抗性。因此，可用作 生物肥料及/或生物殺蟲劑來控制病原性真菌、病毒及細菌之微生物為合乎需要的且高度需要其用以提高農業可持續性。最後一種類型之植物病原體包括植物病原性線蟲及昆蟲，其可造成植物嚴重損害及損失。 Fungal plant pathogens, including but not limited to, Botrytis spp. (eg Botrytis cinerea ), Fusarium spp. (eg F. oxysporum ) F. graminearum , Rhizoctonia spp. (eg R. solani), Magnaporthe spp. , Mycosphaerella spp . ) , Puccinia spp. (such as P. recondita ), Phytopthora spp. and Phakopsora spp. (eg Phaeocysteen rust) ( P. pachyrhizi )) is a type of plant pest that can cause serious economic losses in the agricultural and horticultural industries. Chemical agents can be used to control fungal plant pathogens, but the use of chemical agents has the following disadvantages, including high cost, lack of efficacy, emergence of fungal resistant strains, and undesirable environmental effects. In addition, in addition to targeted plant pathogens, such chemical treatments are often indiscriminate and adversely affect beneficial bacteria, fungi, and arthropods. The second type of plant pest is a bacterial pathogen including, but not limited to, Erwinia spp. (such as Erwinia chrysanthemi ), Pantoea spp. (such as green lemon) P. citrea , Xanthomonas (eg Xanthomonas campestris ), Pseudomonas spp. (such as Pseudomonas syringae ( P) .syringae )) and Ralstonia spp. (such as R. soleacearum ), causing severe economic losses in the agricultural and horticultural industries. Similar to pathogenic fungi, the use of chemical agents to treat such bacterial pathogens has disadvantages. Viruses and virus-like organisms comprise a third type of phytopathogenic agent that is difficult to control, but bacterial microorganisms can provide plant resistance thereto via induction systemic resistance (ISR). Therefore, microorganisms that can be used as biological fertilizers and/or biocides to control pathogenic fungi, viruses and bacteria are desirable and highly desirable for improving agricultural sustainability. The last type of plant pathogen includes phytopathogenic nematodes and insects that can cause serious damage and loss to plants.

已報導芽孢桿菌屬之一些成員為生物控制菌株，且一些已應用於商業產品中(Joseph W.Kloepper等人，2004,Phytopathology第94卷，第11期，1259-1266)。舉例而言，當前用於商業生物控制產品中之菌株包括：地衣芽孢桿菌(Bacillus pumilus)菌株QST2808，用作SONATA及BALLAD-PLUS中之活性成分，由BAYER CROP SCIENCE生產；地衣芽孢桿菌菌株GB34，用作YIELDSHIELD中之活性成分，由BAYER CROP SCIENCE生產；枯草芽孢桿菌(Bacillus subtilis)菌株QST713，用作SERENADE之活性成分，由BAYER CROP SCIENCE生產；枯草芽孢桿菌菌株GBO3，用作KODIAK及SYSTEM3中之活性成分，由HELENA CHEMICAL COMPANY生產。蘇雲金芽孢桿菌(Bacillus thuringiensis)及強固芽孢桿菌(Bacillus firmus)之各種菌株已用作對抗線蟲及病媒昆蟲之生物控制劑，且此等菌株充當許多市售生物控制產品之基礎，該等產品包括NORTICA及PONCHO-VOTIVO，由BAYER CROP SCIENCE生產。另外，當前用於商業生物刺激產品中之芽孢桿菌菌株包括：解澱粉芽孢桿菌(Bacillus amyloliquefaciens)菌株FZB42，用作RHIZOVITAL 42中之活性成分，由ABiTEP GmbH生產；以及各種其他枯草芽孢桿菌屬，包括其作為全細胞，包括其在生物刺激產品中之醱酵提取物，諸如由JHBiotech Inc.生產之FULZYME。 Some members of the genus Bacillus have been reported to be biologically controlled strains, and some have been used in commercial products (Joseph W. Kloepper et al., 2004, Phytopathology Vol. 94, No. 11, 1259-1266). For example, strains currently used in commercial biological control products include: Bacillus pumilus strain QST2808, which is used as an active ingredient in SONATA and BALLAD-PLUS, produced by BAYER CROP SCIENCE; Bacillus licheniformis strain GB34, Used as an active ingredient in YIELDSHIELD, produced by BAYER CROP SCIENCE; Bacillus subtilis strain QST713, used as an active ingredient of SERENADE, produced by BAYER CROP SCIENCE; Bacillus subtilis strain GBO3, used as KODIAK and SYSTEM3 The active ingredient is produced by HELENA CHEMICAL COMPANY. Various strains of Bacillus thuringiensis and Bacillus firmus have been used as biological control agents against nematodes and vector insects, and these strains serve as the basis for many commercially available biological control products, including NORTICA and PONCHO-VOTIVO, produced by BAYER CROP SCIENCE. In addition, the current Bacillus strains used in commercial biostimulation products include: Bacillus amyloliquefaciens strain FZB42, which is used as an active ingredient in RHIZOVITAL 42, produced by ABiTEP GmbH; and various other Bacillus subtilis, It includes it as a whole cell, including its fermentation extract in a biostimulating product, such as FULZYME manufactured by JH Biotech Inc.

本發明揭示之標的物提供微生物組合物及其於使植物生長獲益及預防及控制疾病之使用方法。 The subject matter disclosed herein provides a microbial composition and methods of use thereof for benefiting plant growth and preventing and controlling diseases.

在一個實施例中，提供一種包含以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物的組合物，該組合物用於施用於植物以達成使植物生長獲益或對易感病植物賦予保護以避免病原性感染中的一或兩者。 In one embodiment, there is provided a composition comprising a biologically pure culture of Bacillus amyloliquefaciens RTI472 deposited with ATCC PTA-121166 or a mutant having all of its identifying characteristics, for use in application to a plant To achieve benefits from plant growth or to confer protection to susceptible plants to avoid one or both of the pathogenic infections.

在一個實施例中，提供一種用包含以ATCC第PTA-121166號寄存之之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物之孢子的組合物塗佈之植物種子，該組合物以適合於使植物生長獲益及/或對易感病植物賦予保護以避免病原性感染之量存在。含有解澱粉芽孢桿菌RTI472菌株之組合物可單獨或與其他微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑、除草劑、植物提取物、植物生長調節劑或肥料組合施用。 In one embodiment, there is provided a plant seed coated with a composition comprising a spore of a biologically pure culture of a Bacillus amyloliquefaci RTI472 deposited with ATCC No. PTA-121166 or a mutant having all of its identifying characteristics The composition is present in an amount suitable to benefit plant growth and/or to confer protection to susceptible plants to avoid pathogenic infections. The composition comprising the B. amyloliquefaciens RTI 472 strain can be administered alone or in combination with other microbial, biological or chemical insecticides, fungicides, nematicides, bactericides, herbicides, plant extracts, plant growth regulators or fertilizers. .

在一個實施例中，提供一種用於達成使植物生長獲益及/或對易感病植物賦予保護以避免病原性感染中之一或兩者的組合物，該組合物包含以下各物：以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物，呈適合於使植物生長獲益及/或對易感病植物賦予保護以避免病原性感染之量；以及微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑、除草劑、植物提取物、植物生長調節劑或肥料之一或組合，呈適合於使植物生長獲益及/或對易感病植物賦予保護以避免病原性感染之量。 In one embodiment, a composition for achieving one or both of plant growth benefits and/or protection of a susceptible plant to avoid a pathogenic infection is provided, the composition comprising: ATCC PTA-121166, a biologically pure culture of Bacillus amyloliquefaciens RTI472 or a mutant having all of its identifying characteristics, is suitable for benefiting plant growth and/or conferring protection to susceptible plants to avoid pathogens The amount of sexual infection; and one or a combination of microbial, biological or chemical insecticides, fungicides, nematicides, fungicides, herbicides, plant extracts, plant growth regulators or fertilizers, suitable for plant growth Benefit and/or protect susceptible plants from pathogenic infections.

在一個實施例中，提供一種用於達成使植物生長獲益或對易感病植物賦予保護以避免病原性感染中之一或兩者的方法，該方法包含：將一種組合物輸送至植物葉、植物莖皮、植物果實、植物花、植物種子、植物根部、植物剪枝、植物移植物、植物癒合組織；植物周圍土壤或生長介質；在土壤或生長介質中播種植物種子之前的土壤或生長介質；或在土壤或生長介質中種植植物、植物剪枝、植物移植物或植物癒合組織之前的土壤或生長介質，該組合物包含以ATCC第 PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物，呈適合於使植物生長獲益及/或對易感病植物賦予保護以避免病原性感染之量。 In one embodiment, a method for achieving one or both of benefiting plant growth or conferring protection to a susceptible plant to avoid a pathogenic infection is provided, the method comprising: delivering a composition to a plant leaf , plant bark, plant fruit, plant flower, plant seed, plant root, plant pruning, plant graft, plant healing tissue; soil or growth medium around the plant; soil or growth before plant seed in soil or growth medium a medium or a medium or growth medium prior to planting a plant, plant pruning, plant graft, or plant healing tissue in soil or growth medium, the composition comprising ATCC A biologically pure culture of Bacillus amyloliquefacidis RTI472 or a mutant having all of its identifying characteristics deposited under No. PTA-121166, which is suitable for plant growth benefit and/or protection of susceptible plants to avoid pathogenic infections The amount.

在一個實施例中，提供一種用於達成使植物生長獲益或對易感病植物賦予保護以避免病原性感染中之一或兩者的方法，該方法包含：將一種組合物輸送至植物葉、植物莖皮、植物果實、植物花、植物種子、植物根部、植物剪枝、植物移植物、植物癒合組織；植物周圍土壤或生長介質；在土壤或生長介質中播種植物種子之前的土壤或生長介質；或在土壤或生長介質中種植植物、植物剪枝、植物移植物或植物癒合組織之前的土壤或生長介質，該組合物包含以下各物：以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物，呈適合於使植物生長獲益及/或賦予保護以避免病原性感染之量；以及微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑、除草劑、植物提取物、植物生長調節劑或肥料之一或組合，呈適合於使植物生長獲益及/或賦予保護以避免病原性感染之量。 In one embodiment, a method for achieving one or both of benefiting plant growth or conferring protection to a susceptible plant to avoid a pathogenic infection is provided, the method comprising: delivering a composition to a plant leaf , plant bark, plant fruit, plant flower, plant seed, plant root, plant pruning, plant graft, plant healing tissue; soil or growth medium around the plant; soil or growth before plant seed in soil or growth medium a medium or a soil or growth medium prior to planting a plant, plant pruning, plant graft or plant healing tissue in soil or growth medium, the composition comprising the following: a starch spore deposited under ATCC PTA-121166 Bacillus RTI472 or a biologically pure culture of a mutant having all of its identifying characteristics, in an amount suitable for benefiting plant growth and/or conferring protection against pathogenic infection; and microbial, biological or chemical insecticides, killing One or a combination of fungicides, nematicides, fungicides, herbicides, plant extracts, plant growth regulators or fertilizers To the benefit of plant growth and / or confer protection against the amount of pathogenic infection.

在一個實施例中，提供一種用於達成使植物生長獲益或對易感病植物賦予保護以避免病原性感染中之一或兩者的方法，該方法包含：將第一組合物與第二組合物之組合輸送至植物葉、植物莖皮、植物果實、植物花、植物種子、植物根部、植物剪枝、植物移植物、植物癒合組織；植物周圍土壤或生長介質；在土壤或生長介質中播種植物種子之前的土壤或生長介質；或在土壤或生長介質中種植植物、植物剪枝、植物移植物或植物癒合組織之前的土壤或生長介質，該第一組合物包含以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物，呈適合於使植物生長獲益及/或對易感病植物賦予保護以避免病原性感染之量；且該第二組合物包含微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺 菌劑、除草劑、植物提取物、植物生長調節劑或肥料之一或組合，呈適合於使植物生長獲益及/或對易感病植物賦予保護以避免病原性感染之量。 In one embodiment, a method for achieving one or both of benefiting plant growth or conferring protection to a susceptible plant to avoid a pathogenic infection is provided, the method comprising: treating the first composition with the second The combination of the composition is delivered to plant leaves, plant bark, plant fruits, plant flowers, plant seeds, plant roots, plant pruning, plant transplants, plant healing tissues; soil or growth medium surrounding the plant; in soil or growth medium The soil or growth medium prior to seeding the plant seed; or the soil or growth medium prior to planting the plant, plant pruning, plant graft or plant healing tissue in the soil or growth medium, the first composition comprising ATCC PTA-121166 A biologically pure culture of the deposited B. amyloliquefaciens RTI472 or a mutant thereof having all of its identifying characteristics, in an amount suitable to benefit plant growth and/or to confer protection to susceptible plants to avoid pathogenic infections; And the second composition comprises a microbial, biological or chemical insecticide, a fungicide, a nematicide, a kill One or a combination of a microbial agent, a herbicide, a plant extract, a plant growth regulator or a fertilizer is in an amount suitable to benefit plant growth and/or to confer protection to a susceptible plant to avoid pathogenic infection.

在一個實施例中，提供一種用於達成使植物生長獲益或對易感病植物賦予保護以避免病原性感染中之一或兩者的方法，該方法包含：將植物種子種植在適合生長介質中或使植物之營養剪枝/組織在適合生長介質中再生，其中種子已用一種組合物塗佈或營養剪枝/組織業經該組合物接種，該組合物包含以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物，其中使植物自種子或營養剪枝/組織之生長獲益及/或賦予保護以避免病原性感染。 In one embodiment, a method for achieving one or both of benefiting plant growth or conferring protection to a susceptible plant to avoid a pathogenic infection is provided, the method comprising: planting the plant seed in a suitable growth medium The nutrient pruning/tissue of the plant is regenerated in a suitable growth medium, wherein the seed has been coated with a composition or the nutrient pruning/tissue is inoculated with the composition, the composition comprising the deposit with ATCC PTA-121166 A biologically pure culture of Bacillus amyloliquefaciens RTI 472 or a mutant thereof having all of its identifying characteristics, wherein plants are benefited from the growth of seeds or nutrient pruning/tissue and/or confer protection to avoid pathogenic infections.

在一個實施例中，提供一種藉由對易感病植物賦予保護以避免病原性感染或減少病原性感染使植物生長獲益，同時使針對處理之抗性積聚減至最少的方法，該方法包含：將第一組合物與第二組合物以分開施用及改變之時間間隔輸送至易感病植物，其中該第一組合物及該第二組合物各者以適合於對植物賦予保護以避免或減少病原性感染之量輸送，其中該第一組合物包含以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物，且其中該第二組合物包含一或多種具有殺真菌或殺菌特性之化學活性劑，且其中該第一組合物及該第二組合物以改變之時間間隔輸送至以下之一或組合：植物葉、植物莖皮、植物果實、植物花、植物種子、植物根部、植物剪枝、植物移植物、植物癒合組織或植物周圍土壤或生長介質，其中賦予保護以避免及/或減少病原性感染所需之化學活性劑之總量減少且針對處理之抗性積聚減至最少。 In one embodiment, a method of providing plant protection by protecting a susceptible plant from pathogenic infection or reducing pathogenic infection while minimizing resistance accumulation for treatment is provided, the method comprising : delivering the first composition and the second composition to the susceptible plant at separate intervals of application and alteration, wherein the first composition and the second composition are each adapted to confer protection to the plant to avoid or Reducing the delivery of a pathogenic infection, wherein the first composition comprises a B. amyloliquefaci RTI 472 deposited with ATCC PTA-121166 or a biologically pure culture thereof having all of its identifying characteristics, and wherein the second The composition comprises one or more chemical active agents having fungicidal or bactericidal properties, and wherein the first composition and the second composition are delivered to one or a combination of the following at varying time intervals: plant leaves, plant stems, Plant fruit, plant flower, plant seed, plant root, plant pruning, plant graft, plant healing tissue or soil or growth medium around the plant, Protection against and / or decrease the amount of chemical needed to pathogenic infections and to reduce the accumulation of active agents for the treatment of resistance to be minimized.

在本發明之一個實施例中，提供一種組合物，該組合物包括以下中之至少一者：分離之豐原素MA化合物、分離之豐原素MB化合物、分離之豐原素MC化合物、分離之去羥基豐原素MA化合物、分離 之去羥基豐原素MB化合物、分離之豐原素H化合物、分離之豐原素I化合物及分離之去羥基豐原素I化合物，呈適合於達成使植物生長獲益或對易感病植物賦予保護以避免病原性感染中之一或兩者的量，該等豐原素及去羥基豐原素化合物具有下式： In one embodiment of the present invention, there is provided a composition comprising at least one of the following: isolated abundance MA compound, isolated abundance MB compound, isolated abundance MC compound, isolated dehydroxyl group The abundance MA compound, the isolated hydroxytobarenin MB compound, the isolated abundance H compound, the isolated abundance I compound, and the isolated hydroxy-hydroxytoxin I compound are suitable for achieving plant growth benefit or The diseased plants confer protection to avoid the amount of one or both of the pathogenic infections, which have the following formula:

其中R為OH，n在8至20範圍內，FA為直鏈、異或反異，且：對於豐原素MA，X1為Ala，X2為Thr，且X3為Met；對於豐原素MB，X1為Val，X2為Thr，且X3為Met；對於豐原素MC，X1為Aba，X2為Thr，且X3為Met；對於豐原素H，X1為Val，X2為Thr，且X3為Hcy；且對於豐原素I，X1為Ile，X2為Thr，且X3為Ile；或其中R為H，n在8至20範圍內，FA為直鏈、異或反異，且：對於去羥基豐原素MA，X1為Ala，X2為Thr，且X3為Met；對於去羥基豐原素MB，X1為Val，X2為Thr，且X3為Met；且對於去羥基豐原素I，X1為Ile，X2為Thr，且X3為Ile。 Wherein R is OH, n is in the range of 8 to 20, FA is linear, XOR or di-isomeric, and: for abundance MA, X 1 is Ala, X 2 is Thr, and X 3 is Met; for abundance MB X 1 is Val, X 2 is Thr, and X 3 is Met; for abundance MC, X 1 is Aba, X 2 is Thr, and X 3 is Met; for abundance H, X 1 is Val, X 2 Is Thr and X 3 is Hcy; and for abundance I, X 1 is Ile, X 2 is Thr, and X 3 is Ile; or wherein R is H, n is in the range of 8 to 20, and FA is linear, XOR or singular, and: for dehydroxylectin MA, X 1 is Ala, X 2 is Thr, and X 3 is Met; for dehydroxyl-rich MB, X 1 is Val, X 2 is Thr, and X 3 Is Met; and for dehydroxylectin I, X 1 is Ile, X 2 is Thr, and X 3 is Ile.

在一個實施例中，提供一種組合物，其包含分子量等於3341.6Da之分離之Ericin S化合物及分子量等於2985.4Da之分離之Ericin A化合物中的一或兩者，呈適合於對易感病植物賦予保護以避免病原性感染之量。 In one embodiment, a composition is provided comprising one or both of an isolated Ericin S compound having a molecular weight equal to 3341.6 Da and an isolated Ericin A compound having a molecular weight equal to 2985.4 Da, which is suitable for imparting a susceptible plant Protect to avoid the amount of pathogenic infections.

在一個實施例中，提供以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472之生物純培養物的提取物，該提取物包括豐原素-MA、豐原素-MB、豐原素-MC、豐原素-H及豐原素-I化合物以及去羥基豐 原素-MA、去羥基豐原素-MB及去羥基豐原素-I化合物以及表XVII中列出之其他豐原素樣及去羥基豐原素樣化合物之一或組合。 In one embodiment, an extract of a biologically pure culture of Bacillus amyloliquefaciens RTI472 deposited under ATCC PTA-121166 is provided, the extract comprising amylin-MA, amylin-MB, abundance-MC, Fengyuan素-H and abundance-I compounds and dehydroxyl One or a combination of the protoxin-MA, the dehydroxyhydroxytoxin-MB and the dehydroxylectin-I compound, and the other abundance-like and dehydroxy-norformin-like compounds listed in Table XVII.

在一個實施例中，提供解澱粉芽孢桿菌之生物純培養物之提取物，該提取物包括以下中之至少一者：分子量等於3341.6Da之分離之Ericin S化合物或分子量等於2985.4Da之分離之Ericin A化合物。 In one embodiment, an extract of a biologically pure culture of B. amyloliquefaciens is provided, the extract comprising at least one of the following: an isolated Ericin S compound having a molecular weight equal to 3341.6 Da or an isolated Ericin having a molecular weight equal to 2985.4 Da Compound A.

在一個實施例中，提供一種用於使植物生長獲益之組合物，該組合物包含：以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有所有其鑑別特徵之突變體的生物純培養物；以及畢芬寧(bifenthrin)殺蟲劑。 In one embodiment, a composition for benefiting plant growth is provided, the composition comprising: Bacillus amyloliquefaciens RTI472 deposited with ATCC PTA-121166 or a mutant thereof having all of its identifying characteristics Pure culture; and bifenthrin insecticide.

在一個實施例中，提供一種用於使植物生長獲益之組合物，該組合物包含：以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有所有其鑑別特徵之突變體的生物純培養物；以及包含來自白羽扇豆(Lupinus albus)之提取物、BLAD多肽或BLAD多肽片段之一或組合的殺真菌劑。 In one embodiment, a composition for benefiting plant growth is provided, the composition comprising: Bacillus amyloliquefaciens RTI472 deposited with ATCC PTA-121166 or a mutant thereof having all of its identifying characteristics a pure culture; and a fungicide comprising one or a combination of an extract from a white lupinus ( Lupinus albus ), a BLAD polypeptide or a BLAD polypeptide fragment.

在一個實施例中，提供一種產品，其包含：包含以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物的第一組分；包含微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑、除草劑、植物提取物、植物生長調節劑或肥料之一或組合的第二組分，其中該第一組分及該第二組分分開包裝且其中各組分為適合於達成使植物生長獲益及/或對易感病植物賦予保護以避免病原性感染中之一或兩者之量；以及視情況存在之說明書，該說明書係針對用於將適合於使植物生長獲益之量的第一與第二組合物之組合輸送至：植物葉、植物莖皮、植物果實、植物花、植物種子、植物根部、植物剪枝、植物移植物、植物癒合組織；植物周圍土壤或生長介質；在土壤或生長介質中播種植物種子之前的土壤或生長介質；或在土壤或生長介質中種植植物、植物剪枝、植物移植物 或植物癒合組織之前的土壤或生長介質。 In one embodiment, a product comprising: a first component comprising a biologically pure culture of Bacillus amyloliquefaciens RTI472 deposited with ATCC PTA-121166 or a mutant having all of its identifying characteristics; a second component of one or a combination of a microbial, biological or chemical insecticide, fungicide, nematicide, bactericide, herbicide, plant extract, plant growth regulator or fertilizer, wherein the first component and The second component is packaged separately and wherein the components are suitable for achieving an amount that benefits plant growth and/or confers protection to the susceptible plant to avoid one or both of the pathogenic infection; and optionally The specification is directed to a combination of first and second compositions for use in an amount suitable for plant growth benefit to: plant leaves, plant bark, plant fruits, plant flowers, plant seeds, plant roots, Plant pruning, plant grafts, plant healing tissue; soil or growth medium surrounding the plant; soil or growth medium prior to seeding the plant seed in soil or growth medium; or in soil or Medium long growing plants, pruning plants, plant graft Or the soil or growth medium before the plant heals the tissue.

在一個實施例中，提供一種組合物，該組合物包含：一或多種具有使植物生長或植物健康中之一或兩者獲益之特性的微生物之生物純培養物；以及酵母提取物，用於施用於植物以達成使植物生長獲益或對易感病植物賦予保護以避免病原性感染中之一或兩者。 In one embodiment, a composition is provided comprising: one or more biologically pure cultures of microorganisms having characteristics that benefit one or both of plant growth or plant health; and yeast extracts, Administration to plants to achieve one or both of the benefits of plant growth or protection of susceptible plants to avoid pathogenic infections.

因此概括描述本發明揭示之標的物，現將參考下文描述之附圖。 The subject matter of the present disclosure is therefore summarized, and reference will now be made to the accompanying drawings.

圖1展示根據本發明之一或多個實施例，與兩種解澱粉芽抱桿菌參考菌株解澱粉芽孢桿菌FZB42(中間)及解澱粉芽孢桿菌TrigoCor1448(底部)之對應區域相比，圍繞且包括在解澱粉芽孢桿菌菌株RTI472(頂部)中發現之羊毛硫抗生素生物合成操縱子的基因組結構之示意圖，與RTI472之序列一致性%在各箭頭下展示。進一步分析揭露產生MW=3341.6Da之Ericin S分子及MW為2985.4Da之Ericin A分子的羊毛硫肽合成操縱子，其為解澱粉芽孢桿菌菌株獨特的。 1 shows, in accordance with one or more embodiments of the present invention, surrounding and including compared to corresponding regions of two B. amyloliquefaciens reference strains B. amyloliquefaciens FZB42 (middle) and Bacillus amyloliquefaciens TrigoCor 1448 (bottom) A schematic representation of the genomic structure of the lantibiotic biosynthetic operon found in B. amyloliquefaciens strain RTI 472 (top), and the sequence identity % with RTI 472 is shown under each arrow. Further analysis revealed a thiol peptide synthesis operon that produced an Ericin S molecule with MW = 3341.6 Da and an Ericin A molecule with a MW of 2985.4 Da, which is unique to the B. amyloliquefaciens strain.

圖2A為解澱粉芽孢桿菌RTI472菌株及稱為「FB005」之未公開之解澱粉芽抱桿菌菌株的代謝物型態之圖，其展示RTI472菌株產生Ericin S分子(MW=3341.6Da)及Ericin A分子(MW=2985.4Da)，而對於FB005菌株，未偵測到Ericin A或Ericin S。圖2B為展示在RTI472菌株與7種參考解澱粉芽孢桿菌基因組中鑑別之羊毛硫肽生物合成簇之間的比較之表，其展示在7種參考解澱粉芽孢桿菌基因組之區域中同線性喪失。此羊毛硫肽生物合成簇在RTI472菌株中鑑別為Ericin S生物合成簇，而7種參考菌株中無一者具有功能性Ericin S生物合成簇。圖2C為展示在RTI472菌株與7種參考解澱粉芽孢桿菌基因組中鑑別之羊毛硫肽生物合成簇之間的比較之表，其展示在7種參考解澱粉芽孢桿菌基因組之區域中同線性喪失。此羊毛硫肽生物合成簇在RTI472菌 株中鑑別為Ericin S生物合成簇，而7種參考菌株中無一者具有功能性Ericin S生物合成簇。此等圖展示根據本發明之一或多個實施例，代謝物型態與在其他參考解澱粉芽孢桿菌中不存在的在RTI472菌株中鑑別為Ericin S生物合成簇的羊毛硫肽生物合成簇之間的比較。 2A is a diagram showing the metabolite profile of the B. amyloliquefaciens RTI472 strain and the undisclosed B. amyloliquefaciens strain called "FB005", which shows that the RTI472 strain produces Ericin S molecule (MW=3341.6Da) and Ericin A. Molecule (MW = 2985.4 Da), whereas for FB005 strain, Ericin A or Ericin S was not detected. Figure 2B is a table showing a comparison between the RTI 472 strain and the seven reference ruminant biosynthesis clusters identified in the B. amyloliquefaciens genome, showing a linear loss in the region of the seven reference B. amyloliquefaciens genomes. This lanthiopeptide biosynthesis cluster was identified as an Ericin S biosynthetic cluster in the RTI 472 strain, while none of the seven reference strains had a functional Ericin S biosynthetic cluster. Figure 2C is a table showing a comparison between the RTI 472 strain and the seven reference ruminant biosynthesis clusters identified in the B. amyloliquefaciens genome, showing the same linear loss in the region of the seven reference B. amyloliquefaciens genomes. This lanthiopeptide biosynthesis cluster was identified as an Ericin S biosynthetic cluster in the RTI 472 strain, while none of the seven reference strains had a functional Ericin S biosynthetic cluster. These figures show that the metabolite profile is in accordance with one or more embodiments of the invention, and the thiol biosynthesis cluster identified as an Ericin S biosynthetic cluster in the RTI 472 strain that is not present in other reference B. amyloliquefaciens Comparison between.

圖3為展示根據本發明之一或多個實施例，在用以下各者處理後，感染豆銹病(菜豆銹病菌(Uromyces appendiculatus))10天後y軸上疾病控制%(平均值)的柱狀圖：廢醱酵液(SFB)中RTI472孢子(以1×108cfu/mL施用)、TACTIC(以0.1875%施用於所有調配物且亦用作空白對照)、得克利(Tebuconazole)(以50g a.i/ha施用)、四氯異苯腈(以500g a.i./ha施用)、SERENADE OPTIMUM(以1×108cfu/ml施用)及SERENADE OPTIMUM(以4×108cfu/ml施用)。未經處理之對照產生23%疾病。後面為相同字母之值不顯著不同(p=0.10)。 Figure 3 is a graph showing the % of disease control (average value) on the y-axis after 10 days of infection with soybean rust ( Uromyces appendiculatus ) after treatment with each of the following in accordance with one or more embodiments of the present invention. Plot: RTI 472 spores (administered at 1 x 10 8 cfu/mL), TACTIC (0.1875% applied to all formulations and also used as a blank control), Tebuconazole (in the case of waste broth (SFB)) 50 g ai/ha application), tetrachloroisophthalonitrile (administered at 500 g ai/ha), SERENADE OPTIMUM (administered at 1 x 10 8 cfu/ml) and SERENADE OPTIMUM (administered at 4 x 10 8 cfu/ml). Untreated controls produced 23% of the disease. The values followed by the same letter are not significantly different (p=0.10).

圖4為展示根據本發明之一或多個實施例，在用以下各者處理後，與感染白粉病菌(Microsphaera diffusa)之大豆植物鄰接置放9天後大豆植物中y軸上疾病控制%(平均值)的柱狀圖：廢醱酵液(SFB)中RTI472孢子(以1×108cfu/ml施用)、SERENADE OPTIMUM(以1×108cfu/ml施用)及HORIZON(得克利以50g a.i/ha施用)。另外，各處理包括作為佐劑之TACTIC(以0.1875%施用)。未經處理之對照產生70%疾病嚴重程度。後面為相同字母之值不顯著不同(p=0.10)。 4 is a graph showing % of disease control on the y-axis of soybean plants after 9 days of being placed adjacent to soybean plants infected with Microsphaera diffusa after treatment with each of the following in accordance with one or more embodiments of the present invention ( Histogram of the mean: RTI472 spores (administered at 1×10 8 cfu/ml), SERENADE OPTIMUM (administered at 1×10 8 cfu/ml) and HORIZON in waste broth (SFB) Ai/ha application). In addition, each treatment included TACTIC (administered at 0.1875%) as an adjuvant. Untreated controls produced 70% disease severity. The values followed by the same letter are not significantly different (p=0.10).

圖5為展示在用以下各者處理後，感染胡椒葡萄孢枯萎病(灰葡萄孢菌)7天後y軸上疾病控制%(平均值)的柱狀圖：廢醱酵液(SFB)中RTI472孢子(以1×108cfu/ml施用)、TACTIC(以0.1875%施用於所有調配物且亦用作空白對照)、得克利(以50g a.i/ha施用)、四氯異苯腈(以500g a.i./ha施用)、SERENADE OPTIMUM(以1×108cfu/ml施用)及SERENADE OPTIMUM(以4×108cfu/ml施用)。根據本發明之一或多個實施例，未經處理之對照產生6%疾病。後面為相同字母之值不顯 著不同(p=0.10)。 Figure 5 is a bar graph showing the % control of the disease on the y-axis after 7 days of infection with Botrytis cinerea wilt (B. cinerea) after treatment with the following: Waste broth (SFB) RTI472 spores (administered at 1 x 10 8 cfu/ml), TACTIC (administered to all formulations at 0.1875% and also used as a blank control), Dekli (administered at 50 g ai/ha), tetrachloroisophthalonitrile (in 500 g ai/ha application), SERENADE OPTIMUM (administered at 1 x 10 8 cfu/ml) and SERENADE OPTIMUM (administered at 4 x 10 8 cfu/ml). According to one or more embodiments of the invention, the untreated control produces 6% disease. The values followed by the same letter are not significantly different (p=0.10).

圖6為展示根據本發明之一或多個實施例，在用以下各者處理後，感染胡椒葡萄孢枯萎病(灰葡萄孢菌)3天後y軸上疾病控制%(平均值)的柱狀圖：與TACTIC(以0.1875%施用於所有調配物且亦用作空白對照)、SERENADE OPTIMUM(以4×108cfu/ml施用)及得克利(以50g a.i/ha施用)相比，廢醱酵液(SFB)中RTI472孢子(以2.5×106、1×107、2.5×107、1×108及2.5×108cfu/ml施用)及SERENADE OPTIMUM(以2.5×106、1×107、2.5×107、1×108及2.5×108cfu/ml施用)。未經處理之對照產生30%疾病。後面為相同字母之值不顯著不同(p=0.10)。 Figure 6 is a graph showing the % control of the disease on the y-axis after 3 days of infection with Botrytis cinerea wilt (B. cinerea), after treatment with each of the following, in accordance with one or more embodiments of the present invention. Plot: compared to TACTIC (administered to all formulations at 0.1875% and also used as a blank), SERENADE OPTIMUM (administered at 4 x 10 8 cfu/ml) and Dekli (administered at 50 g ai/ha) RTI472 spores in sputum (SFB) (administered at 2.5 × 10 6 , 1 × 10 7 , 2.5 × 10 7 , 1 × 10 8 and 2.5 × 10 8 cfu / ml ) and SERENADE OPTIMUM (with 2.5 × 10 6 , 1 × 10 7 , 2.5 × 10 7 , 1 × 10 8 and 2.5 × 10 8 cfu / ml application). Untreated controls produced 30% of the disease. The values followed by the same letter are not significantly different (p=0.10).

圖7為展示根據本發明之一或多個實施例，在用以下各者處理後，感染增加量之胡椒葡萄孢枯萎病(灰葡萄孢菌)4天後y軸上疾病控制%(平均值)的柱狀圖：與TACTIC(以0.1875%施用於所有調配物且亦用作空白對照)及得克利(HORIZON；以50g a.i/ha施用)相比，廢醱酵液(SFB)中RTI472孢子(以1.0×108cfu/ml施用)及SERENADE OPTIMUM(以1×108cfu/ml及4×108cfu/ml施用)。未經處理之對照中作為感染增加量之灰葡萄孢菌(分生孢子/毫升)之函數的疾病%分別為50k=15%，100k=30%，500k=65%，1M=65%，且2M=65%。後面為相同字母之值不顯著不同(p=0.10)。 Figure 7 is a graph showing % of disease control on the y-axis after 4 days of infection with an increased amount of infection, Botrytis cinerea wilt (B. cinerea), after treatment with each of the following, in accordance with one or more embodiments of the present invention. Histogram: RTI472 spores in spent broth (SFB) compared to TACTIC (0.1875% applied to all formulations and also used as a blank control) and Dicki (HORIZON; applied at 50g ai/ha) (administered at 1.0 x 10 8 cfu/ml) and SERENADE OPTIMUM (administered at 1 x 10 8 cfu/ml and 4 x 10 8 cfu/ml). The % of disease as a function of the increased amount of infection of Botrytis cinerea (conidia/ml) in the untreated control was 50k = 15%, 100k = 30%, 500k = 65%, 1M = 65%, respectively. 2M = 65%. The values followed by the same letter are not significantly different (p=0.10).

圖8A為根據本發明之一或多個實施例，在用SERENADE OPTIMUM(以1×108cfu/ml施用)處理之後，感染50k分生孢子/毫升之胡椒葡萄孢枯萎病(灰葡萄孢菌)4天後胡椒植物之影像。圖8B為根據本發明之一或多個實施例，在用廢醱酵液(SFB)中RTI472孢子(以1×108cfu/ml施用)處理之後，感染50k分生孢子/毫升之胡椒葡萄孢枯萎病(灰葡萄孢菌)4天後胡椒植物之影像。圖8C為根據本發明之一或多個實施例，在用TACTIC(以0.1875%施用於所有調配物且亦用作空白對照)處理之後，感染50k分生孢子/毫升之胡椒葡萄孢枯萎病(灰葡 萄孢菌)4天後胡椒植物之影像。圖8D為根據本發明之一或多個實施例，在用感染對照處理之後，感染50k分生孢子/毫升之胡椒葡萄孢枯萎病(灰葡萄孢菌)4天後胡椒植物之影像。 Figure 8A is a Botrytis cinerea wilt (50 Botrytis cinerea) infected with 50 k of conidia/ml after treatment with SERENADE OPTIMUM (administered at 1 x 10 8 cfu/ml) in accordance with one or more embodiments of the present invention. ) Image of pepper plants after 4 days. Figure 8B is a 50k conidia/ml pepper grape infected with RTI472 spores (administered at 1 x 10 8 cfu/ml) in spent broth (SFB), in accordance with one or more embodiments of the present invention. Image of pepper plants after 4 days of spore wilt (Botrytis cinerea). Figure 8C is a 50k conidia/ml of Botrytis cinerea wilt after treatment with TACTIC (administered at 0.1875% to all formulations and also as a blank control) in accordance with one or more embodiments of the invention ( Image of pepper plants after 4 days of Botrytis cinerea. Figure 8D is an image of a pepper plant 4 days after infection with 50 k of conidia/ml of Botrytis cinerea wilt (B. cinerea) after treatment with an infection control, in accordance with one or more embodiments of the present invention.

圖9A為根據本發明之一或多個實施例，在用SERENADE OPTIMUM(以1×108cfu/ml施用)處理之後，感染100k分生孢子/毫升之胡椒葡萄孢枯萎病(灰葡萄孢菌)4天後胡椒植物之影像。圖9B為根據本發明之一或多個實施例，在用廢醱酵液(SFB)中RTI472孢子(以1×108cfu/ml施用)處理之後，感染100k分生孢子/毫升之胡椒葡萄孢枯萎病(灰葡萄孢菌)4天後胡椒植物之影像。圖9C為根據本發明之一或多個實施例，在用TACTIC(以0.1875%施用於所有調配物且亦用作空白對照)處理之後，感染100k分生孢子/毫升之胡椒葡萄孢枯萎病(灰葡萄孢菌)4天後胡椒植物之影像。圖9D為根據本發明之一或多個實施例，在用感染對照處理之後，感染100k分生孢子/毫升之胡椒葡萄孢枯萎病(灰葡萄孢菌)4天後胡椒植物之影像。 9A is a Botrytis cinerea infected with 100 k of conidia/ml after treatment with SERENADE OPTIMUM (administered at 1×10 8 cfu/ml) in accordance with one or more embodiments of the present invention. ) Image of pepper plants after 4 days. Figure 9B is a pepper grape infected with 100k conidia/ml after treatment with RTI472 spores (administered at 1 x 10 8 cfu/ml) in spent broth (SFB), in accordance with one or more embodiments of the present invention. Image of pepper plants after 4 days of spore wilt (Botrytis cinerea). Figure 9C is a staining of 100k conidia/ml of Botrytis cinerea wilt after treatment with TACTIC (administered at 0.1875% to all formulations and also as a blank control) in accordance with one or more embodiments of the invention ( Image of pepper plants after 4 days of Botrytis cinerea. Figure 9D is an image of a pepper plant 4 days after infection with 100 k of conidia/ml of Botrytis cinerea wilt (Botrytis cinerea) after treatment with an infection control, in accordance with one or more embodiments of the present invention.

圖10A為根據本發明之一或多個實施例，在用SERENADE OPTIMUM(以1×108cfu/ml施用)處理之後，感染500k分生孢子/毫升之胡椒葡萄孢枯萎病(灰葡萄孢菌)4天後胡椒植物之影像。圖10B為根據本發明之一或多個實施例，在用廢醱酵液(SFB)中RTI472孢子(以1×108cfu/ml施用)處理之後，感染500k分生孢子/毫升之胡椒葡萄孢枯萎病(灰葡萄孢菌)4天後胡椒植物之影像。圖10C為根據本發明之一或多個實施例，在用TACTIC(以0.1875%施用於所有調配物且亦用作空白對照)處理之後，感染500k分生孢子/毫升之胡椒葡萄孢枯萎病(灰葡萄孢菌)4天後胡椒植物之影像。圖10D為根據本發明之一或多個實施例，在用感染對照處理之後，感染500k分生孢子/毫升之胡椒葡萄孢枯萎病(灰葡萄孢菌)4天後胡椒植物之影像。 Figure 10A is a Botrytis cinerea wilt (500 Botrytis cinerea) infected with 500 k of conidia/ml after treatment with SERENADE OPTIMUM (administered at 1 x 10 8 cfu/ml) in accordance with one or more embodiments of the present invention. ) Image of pepper plants after 4 days. Figure 10B is a pepper grape infected with 500k conidia/ml after treatment with RTI472 spores (administered at 1 x 10 8 cfu/ml) in spent broth (SFB), in accordance with one or more embodiments of the present invention. Image of pepper plants after 4 days of spore wilt (Botrytis cinerea). Figure 10C is a 500k conidia/ml of Botrytis cinerea wilt after treatment with TACTIC (0.1875% applied to all formulations and also as a blank control) in accordance with one or more embodiments of the invention ( Image of pepper plants after 4 days of Botrytis cinerea. Figure 10D is an image of a pepper plant 4 days after infection with 500 k of conidia/ml of Botrytis cinerea wilt (B. cinerea) after treatment with an infection control, in accordance with one or more embodiments of the present invention.

圖11A為根據本發明之一或多個實施例，在用SERENADE OPTIMUM(以1×108cfu/ml施用)處理之後，感染1M分生孢子/毫升之胡椒葡萄孢枯萎病(灰葡萄孢菌)4天後胡椒植物之影像。圖11B為根據本發明之一或多個實施例，在用廢醱酵液(SFB)中RTI472孢子(以1×108cfu/ml施用)處理之後，感染1M分生孢子/毫升之胡椒葡萄孢枯萎病(灰葡萄孢菌)4天後胡椒植物之影像。圖11C為根據本發明之一或多個實施例，在用TACTIC(以0.1875%施用於所有調配物且亦用作空白對照)處理之後，感染1M分生孢子/毫升之胡椒葡萄孢枯萎病(灰葡萄孢菌)4天後胡椒植物之影像。圖11D為根據本發明之一或多個實施例，在用感染對照處理之後，感染1M分生孢子/毫升之胡椒葡萄孢枯萎病(灰葡萄孢菌)4天後胡椒植物之影像。 Figure 11A is a staining of 1 M conidia/ml of Botrytis cinerea wilt (S. cinerea) after treatment with SERENADE OPTIMUM (administered at 1 x 10 8 cfu/ml) in accordance with one or more embodiments of the present invention. ) Image of pepper plants after 4 days. Figure 11B is a pepper grape infected with 1 M conidia/ml after treatment with RTI472 spores (administered at 1 x 10 8 cfu/ml) in spent broth (SFB), in accordance with one or more embodiments of the present invention. Image of pepper plants after 4 days of spore wilt (Botrytis cinerea). Figure 11C is a diagram of infection with 1 M conidia/ml of Botrytis cinerea wilt after treatment with TACTIC (administered at 0.1875% to all formulations and also as a blank control) in accordance with one or more embodiments of the invention ( Image of pepper plants after 4 days of Botrytis cinerea. Figure 11D is an image of a pepper plant 4 days after infection with 1 M conidia/ml of Botrytis cinerea wilt (B. cinerea) after treatment with an infection control, in accordance with one or more embodiments of the present invention.

圖12A為根據本發明之一或多個實施例，在用SERENADE OPTIMUM(以1×108cfu/ml施用)處理之後，感染2M分生孢子/毫升之胡椒葡萄孢枯萎病(灰葡萄孢菌)4天後胡椒植物之影像。圖12B為根據本發明之一或多個實施例，在用廢醱酵液(SFB)中RTI472孢子(以1×108cfu/ml施用)處理之後，感染2M分生孢子/毫升之胡椒葡萄孢枯萎病(灰葡萄孢菌)4天後胡椒植物之影像。圖12C為根據本發明之一或多個實施例，在用TACTIC(以0.1875%施用於所有調配物且亦用作空白對照)處理之後，感染2M分生孢子/毫升之胡椒葡萄孢枯萎病(灰葡萄孢菌)4天後胡椒植物之影像。圖12D為根據本發明之一或多個實施例，在用感染對照處理之後，感染2M分生孢子/毫升之胡椒葡萄孢枯萎病(灰葡萄孢菌)4天後胡椒植物之影像。 Figure 12A is an infection of 2M conidia/ml of Botrytis cinerea wilt (S. cinerea) after treatment with SERENADE OPTIMUM (administered at 1 x 10 8 cfu/ml) in accordance with one or more embodiments of the present invention. ) Image of pepper plants after 4 days. Figure 12B is a diagram showing the infection of 2M conidia/ml pepper grape after treatment with RTI472 spores (administered at 1 x 10 8 cfu/ml) in spent broth (SFB) according to one or more embodiments of the present invention. Image of pepper plants after 4 days of spore wilt (Botrytis cinerea). Figure 12C is a staining of 2M conidia/ml of Botrytis cinerea wilt after treatment with TACTIC (administered at 0.1875% to all formulations and also as a blank control) in accordance with one or more embodiments of the invention ( Image of pepper plants after 4 days of Botrytis cinerea. Figure 12D is an image of a pepper plant 4 days after infection with 2M conidia/ml of Botrytis cinerea wilt (Botrytis cinerea) after treatment with an infection control, in accordance with one or more embodiments of the present invention.

圖13展示根據本發明之一或多個實施例，在確定RTI472菌株對抗灰葡萄孢菌病原體之拮抗作用的四個獨立草莓田間試驗各者中未經處理之對照(「UT」)中感染此病原體之果實%的圖。 Figure 13 shows infection in an untreated control ("UT") in each of four independent strawberry field trials determining antagonism of RTI 472 strain against Botrytis cinerea pathogen in accordance with one or more embodiments of the present invention. A graph of the % of the fruit of the pathogen.

圖14為展示由包括解澱粉芽孢桿菌之微生物物種產生的先前報導之豐原素型及去羥基豐原素型環狀脂肽，及由根據本發明之一或多 個實施例，解澱粉芽孢桿菌RTI472分離株產生之新鑑別(以粗體展示)之豐原素型及去羥基豐原素型分子的示意圖。 Figure 14 is a graph showing the previously reported abundance of protosanoids and dehydroxyhydroxytoxin-type cyclic lipopeptides produced by a microbial species including Bacillus amyloliquefaciens, and by Bacillus amyloliquefaciens RTI472 according to one or more embodiments of the present invention Schematic representation of the new identification (shown in bold) of the isolates and the dehydroxyl-rich molecules.

圖15為展示根據本發明之一或多個實施例，在酸沈澱之後自RTI472廢醱酵液(SFB)回收之脂肽百分比的圖。術語「472-AP-球粒」及「472-AP-上清液」分別指在離心之SFB進行酸沈澱之後獲得的再懸浮球粒及上清液。計算百分比且基於來自RTI472廢醱酵液(472-SFB)之各脂肽之積分離子豐度比較。 15 is a graph showing the percentage of lipopeptides recovered from RTI 472 waste mash (SFB) after acid precipitation, in accordance with one or more embodiments of the present invention. The terms "472-AP-spherule" and "472-AP-supernatant" refer to the resuspended pellets and supernatant obtained after acid precipitation of SFB in centrifugation, respectively. The percentage was calculated and based on the integrated ion abundance comparison of each lipopeptide from RTI 472 waste broth (472-SFB).

圖16A展示在具有RTI472廢醱酵液(472-SFB)之869瓊脂盤上點樣之灰葡萄孢菌。圖16B展示在具有在酸沈澱加上離心之後獲得的再懸浮球粒物質(472-AP-球粒)之869瓊脂盤上點樣之灰葡萄孢菌。圖16C展示在具有RTI472廢醱酵液(472-SFB)之869瓊脂盤上點樣之禾穀鐮刀菌。圖16D展示在具有在酸沈澱加上離心之後獲得的再懸浮球粒物質(472-AP-球粒)之869瓊脂盤上點樣之禾穀鐮刀菌。圖16A-16D為展示本發明之一或多個實施例，藉由解澱粉芽孢桿菌RTI472之廢醱酵液(SFB)之酸沈澱中的分離代謝物控制灰葡萄孢菌及禾穀鐮刀菌的盤分析影像。對於陽性對照，10μl RTI472細胞培養物點樣在各培養盤左側，緊挨真菌。 Figure 16A shows the Botrytis cinerea spotted on a 869 agar plate with RTI 472 waste broth (472-SFB). Figure 16B shows Botrytis cinerea on a 869 agar plate with resuspended pellet material (472-AP-spherules) obtained after acid precipitation plus centrifugation. Figure 16C shows Fusarium graminearum spotted on a 869 agar plate with RTI 472 waste broth (472-SFB). Figure 16D shows Fusarium graminearum spotted on a 869 agar plate with resuspended pellet material (472-AP-spherules) obtained after acid precipitation plus centrifugation. 16A-16D are diagrams showing the control of Botrytis cinerea and Fusarium graminearum by an isolated metabolite in the acid precipitation of the detoxification solution (SFB) of Bacillus amyloliquefaciens RTI472, in accordance with one or more embodiments of the present invention. Disc analysis of images. For the positive control, 10 μl of RTI472 cell culture was spotted on the left side of each plate, next to the fungus.

術語「一(a/an)」及「該」在用於本申請案(包括申請專利範圍)時係指「一或多個」。因此，除非上下文明確相反，否則例如提及「植物」包括複數種植物。 The terms "a/an" and "the" are used in the context of the application (including the scope of the patent application) to mean "one or more." Thus, for example, reference to "a plant" includes a plurality of plants unless the context clearly dictates otherwise.

在本說明書及申請專利範圍通篇中，除上下文另外要求之外，術語「包含(comprise/comprises/comprising)」以非排斥含義使用。同樣，術語「包括」及其語法變化形式意欲為非限制性的，使得清單中項目之列舉不排除可取代或添加至所列項目中之其他類似項目。 Throughout the specification and claims, the term "comprise/comprises/comprising" is used in a non-exclusive sense, unless the context requires otherwise. Similarly, the term "comprise" and its grammatical variations are intended to be non-limiting, such that the listing of items in the list does not exclude other similar items that can be substituted or added to the listed items.

出於本說明書及申請專利範圍之目的，術語「約」在與一或多 個數字或數值範圍結合使用時應理解為指所有此類數字，包括範圍內之所有數字，且藉由擴展邊界高於及低於所闡述數值來修改彼範圍。由端點列舉之數值範圍包括包含在彼範圍內及彼範圍內任何範圍之所有數字，例如全部整數，包括其分數(例如，列舉1至5包括1、2、3、4及5，以及其分數，例如1.5、2.25、3.75、4.1及其類似者)。 For the purposes of this specification and the scope of the patent application, the term "about" is used with one or more The use of a range of numbers or ranges of values is understood to mean all such numbers, including all numbers in the range, and the extents are modified by extending the boundaries above and below the stated values. The recitation of numerical ranges by endpoints includes all numbers, including all integers, including the integers thereof, including the range of 1 to 5 including 1, 2, 3, 4, and 5, and Scores, such as 1.5, 2.25, 3.75, 4.1, and the like).

出於本說明書及申請專利範圍之目的，術語「代謝物」及「化合物」當與由RTI472菌株或另一解澱粉芽孢桿菌菌株產生之具有抗微生物活性之化合物結合使用時可互換使用。 For the purposes of this specification and the scope of the patent application, the terms "metabolite" and "compound" are used interchangeably when used in combination with a compound having antimicrobial activity produced by a strain of RTI 472 or another strain of B. amyloliquefaciens.

如本文所用，片語「細菌菌株之生物純培養物」係指以下各者之一或組合：細菌菌株之生物純醱酵培養物之孢子；細菌菌株之生物純醱酵培養物之營養細胞；細菌菌株之生物純醱酵培養物之一或多種產物；細菌菌株之生物純醱酵培養物之培養物固體；細菌菌株之生物純醱酵培養物之培養物上清液；細菌菌株之生物純醱酵培養物之提取物；及細菌菌株之生物純醱酵培養物之一或多種代謝物。 As used herein, the phrase "biologically pure culture of a bacterial strain" means one or a combination of: a spore of a biologically pure fermentation culture of a bacterial strain; a nutritional cell of a biologically pure fermentation culture of a bacterial strain; One or more products of a biologically pure fermentation culture of a bacterial strain; a culture solid of a biologically pure fermentation culture of a bacterial strain; a culture supernatant of a biologically pure fermentation culture of a bacterial strain; a biological purity of the bacterial strain An extract of the fermented culture; and one or more metabolites of the biologically pure fermentation of the bacterial strain.

在本發明之某些實施例中，提供包括新鑑別之解澱粉芽孢桿菌菌株之生物純培養物的組合物及方法，其用於施用於植物以達成使植物生長獲益或對易感病植物賦予保護以避免病原性感染中的一或兩者。在本發明之組合物及方法中，植物生長益處藉由幼苗活力提高、根發育改善、植物生長改善、植物健康改善、產量增加、外觀改善、對植物病原體之抗性提高、病原性感染減少或其組合展現。 In certain embodiments of the invention, compositions and methods are provided comprising a biologically pure culture of a newly identified strain of Bacillus amyloliquefaciens for application to a plant to achieve a plant growth benefit or a susceptible plant Give protection to avoid one or both of the pathogenic infections. In the compositions and methods of the present invention, plant growth benefits are enhanced by increased seedling vigor, improved root development, improved plant growth, improved plant health, increased yield, improved appearance, increased resistance to plant pathogens, reduced pathogenic infection, or Its combination is displayed.

鑑別為屬於物種解澱粉芽孢桿菌之植物相關細菌自生長在北卡羅來納州(North Carolina)之西洋參根分離且隨後測試植物病原體拮抗特性。更特定言之，經由高度保守之16S rRNA及rpoB基因之序列分析將分離之細菌菌株鑑別為解澱粉芽孢桿菌菌株之新菌株(參見實例1)。測得新細菌分離株(稱為「解澱粉芽孢桿菌RTI472」)之16S RNA序列與解澱粉芽孢桿菌之三種其他已知菌株解澱粉芽孢桿菌菌株NS6 (KF177175)、解澱粉芽孢桿菌菌株FZB42(NR_075005)及枯草芽孢桿菌亞種(Bacillus subtilis subsp.)枯草菌株DSM 10(NR_027552)的16S rRNA基因序列一致。另外，測得RTI472之rpoB序列具有與已知之解澱粉芽孢桿菌AS43.3菌株最高程度之序列相似性(亦即>99%序列一致性)；然而，在DNA層面上存在10個核苷酸差異，表明該RTI472為解澱粉芽孢桿菌RTI472之新菌株。解澱粉芽孢桿菌RTI472之該菌株於2014年4月17日依據國際承認用於專利程序的微生物寄存布達佩斯條約之條款寄存於美國菌種保藏中心(American Type Culture Collection，ATCC)(Manassas,Virginia,USA)且具有專利寄存編號PTA-121166。 Plant-associated bacteria identified as belonging to the species B. amyloliquefaciens were isolated from the roots of American ginseng grown in North Carolina and subsequently tested for plant pathogen antagonistic properties. More specifically, the isolated bacterial strain was identified as a new strain of B. amyloliquefaciens strain by sequence analysis of the highly conserved 16S rRNA and rpoB genes (see Example 1). The 16S RNA sequence of the new bacterial isolate (called "Bacillus amyloliquefaciens RTI472") and three other known strains of Bacillus amyloliquefaciens, Bacillus amyloliquefaciens strain NS6 (KF177175), Bacillus amyloliquefaciens strain FZB42 (NR_075005) And the 16S rRNA gene sequence of Bacillus subtilis subsp. subtilis strain DSM 10 (NR_027552) was identical. In addition, the rpoB sequence of RTI472 was found to have the highest degree of sequence similarity (ie, >99% sequence identity) to the known B. amyloliquefaciens AS43.3 strain; however, there were 10 nucleotide differences at the DNA level. , indicating that the RTI472 is a new strain of Bacillus amyloliquefaciens RTI472. The strain of Bacillus amyloliquefaciens RTI472 was deposited with the American Type Culture Collection (ATCC) on April 17, 2014 in accordance with the terms of the Budapest Treaty on the International Recognition of Microorganisms for Patent Procedures (Manassas, Virginia, USA). And has the patent registration number PTA-121166.

解澱粉芽孢桿菌RTI472菌株之基因組的進一步序列分析揭露該菌株具有同源物在其他緊密相關解澱粉芽孢桿菌RTI472菌株中缺乏的與羊毛硫抗生素生物合成相關之基因(參見實例2)。此在圖1中說明，其展示在解澱粉芽孢桿菌RTI472中發現之羊毛硫抗生素生物合成簇及兩種已知解澱粉芽孢桿菌參考菌株FZB42(中間)及TrigoCor1448(底部)(展示在RTI472菌株下面)之對應區域之基因組結構的示意圖。自圖1可觀測到FZB42及TrigoCor1448菌株缺乏許多存在於此簇中之基因，且存在之許多基因內序列一致性程度低。RTI472菌株中之此羊毛硫肽生物合成簇經鑑別為Ericin S生物合成簇。使用HPLC/MS/MS分析RTI472菌株之代謝物型態，其展示新鑑別之RTI472菌株產生MW=3341.6Da之Ericin S分子及MW為2985.4Da之Ericin A分子。圖2A展示解澱粉芽孢桿菌RTI472菌株及稱為「FB005」之未公開之解澱粉芽孢桿菌菌株的代謝物型態之圖。該型態展示RTI472菌株產生Ericin A及Ericin S肽，而對於FB005菌株，未偵測到Ericin A或Ericin S。出於本說明書及申請專利範圍之目的，術語「Ericin S」、「Ericin S分子」、「Ericin S化合物」及「Ericin S肽」在本文中可互換使用。 類似地，出於本說明書及申請專利範圍之目的，術語「Ericin A」、「Ericin A分子」、「Ericin A化合物」及「Ericin A肽」在本文中可互換使用。 Further sequence analysis of the genome of Bacillus amyloliquefaciens RTI 472 strain revealed that the strain has a gene associated with lantibiotic biosynthesis that is homologous in other closely related B. amyloliquefaciens RTI 472 strains (see Example 2). This is illustrated in Figure 1, which shows the lantibiotic biosynthetic cluster found in B. amyloliquesii RTI472 and two known B. amyloliquefaciens reference strains FZB42 (middle) and TrigoCor1448 (bottom) (shown under the RTI472 strain) Schematic diagram of the genomic structure of the corresponding region. It can be observed from Figure 1 that the FZB42 and TrigoCor1448 strains lack many of the genes present in this cluster, and many of the genes present have a low degree of sequence identity. This thiochipeptide biosynthesis cluster in the RTI 472 strain was identified as an Ericin S biosynthetic cluster. The metabolite profile of the RTI 472 strain was analyzed using HPLC/MS/MS, which showed that the newly identified RTI 472 strain produced an Ericin S molecule with MW = 3341.6 Da and an Ericin A molecule with a MW of 2985.4 Da. Figure 2A shows a diagram of the metabolite profile of the B. amyloliquefaciens RTI 472 strain and the undisclosed B. amyloliquefaciens strain designated "FB005". This type shows that the RTI472 strain produces Ericin A and Ericin S peptides, whereas for the FB005 strain, Ericin A or Ericin S was not detected. For the purposes of this specification and the scope of the patent application, the terms "Ericin S", "Ericin S molecule", "Ericin S compound" and "Ericin S peptide" are used interchangeably herein. Similarly, for the purposes of this specification and the scope of the patent application, the terms "Ericin A", "Ericin A molecule", "Ericin A compound" and "Ericin A peptide" are used interchangeably herein.

另外，與許多其他解澱粉芽孢桿菌參考菌株之進一步比較基因組學類似地展示此等菌株亦缺乏羊毛硫抗生素生物合成簇。圖2B-2C為展示在RTI472菌株與7種參考解澱粉芽孢桿菌基因組中鑑別之羊毛硫肽生物合成簇之間的比較之表，其展示在7種參考解澱粉芽抱桿菌基因組之區域中同線性喪失。該等資料表明新鑑別之RTI472具有獨特之羊毛硫抗生素生物合成路徑。此羊毛硫肽生物合成簇在該RTI472菌株中鑑別為Ericin S生物合成簇，且7種參考菌株中無一者具有功能性Ericin S生物合成簇。因此，新鑑別之RTI472菌株具有產生MW=3341.6Da之Ericin S分子及MW為2985.4Da之Ericin A分子的羊毛硫肽合成路徑，此為解澱粉芽孢桿菌菌株獨特的。 In addition, similar comparative genomics to many other B. amyloliquefaciens reference strains showed that these strains also lacked the lantibiotic biosynthetic cluster. 2B-2C are tables showing a comparison between the RTI472 strain and the 7 reference B. amyloliquefaciens genomes identified in the lanthiopeptide biosynthesis cluster, which are shown in the region of the seven reference B. aeruginosa genomes. Loss of linearity. These data indicate that the newly identified RTI472 has a unique lantibiotic biosynthetic pathway. This lanthiopeptide biosynthesis cluster was identified as an Ericin S biosynthetic cluster in the RTI472 strain, and none of the seven reference strains had a functional Ericin S biosynthetic cluster. Therefore, the newly identified RTI472 strain has a thiol peptide synthesis pathway which produces an Ericin S molecule of MW = 3341.6 Da and an Ericin A molecule of 2985.4 Da, which is unique to the B. amyloliquefaciens strain.

另外，進行實驗以確定解澱粉芽孢桿菌RTI472菌株在活體外及在各種植物中在變化條件下之促生長及拮抗活性。實驗結果提供於圖3至16及本文實例3至16中。實驗展示與含有枯草芽孢桿菌菌株QST713作為活性成分之市售SERENADE(BAYER CROP SCIENCE,INC)相比，解澱粉芽孢桿菌RTI472能夠使植物生長獲益且賦予保護以避免或控制植物病原性感染。除單獨施用之外，解澱粉芽孢桿菌RTI472菌株亦用於預防/治療植物病原性感染之方案中，其中其與一或多種市售化學殺真菌劑/殺菌劑以交替時間間隔施用於植物。在一些情況下，單獨解澱粉芽孢桿菌RTI472菌株之施用的效能與化學殺真菌劑/殺菌劑同等佳。在一些情況下，RTI472菌株可用於替換方案中之化學活性劑之一。RTI472菌株在用作玉米種子處理劑時亦有效增加產量且減少疾病。實驗結果提供新RTI472菌株增強FRACTURE(CONSUMO EM VERDE(CEV),BIOTECNOLOGIA DAS PLANTAS S.A.,PORTUGAL)之拮抗特性之益處的實例，FRACTURE為含有BLAD多肽作為活性成分之植物提取物。亦描述由RTI472菌株產生之新抗微生物代謝物的鑑別。 In addition, experiments were conducted to determine the growth-promoting and antagonistic activity of the B. amyloliquefaciens RTI472 strain under varying conditions in vitro and in various plants. The experimental results are provided in Figures 3 to 16 and Examples 3 to 16 herein. Experiments show that B. amyloliquefaciens RTI 472 can benefit plant growth and confer protection to avoid or control plant pathogenic infections compared to the commercially available SERENADE (BAYER CROP SCIENCE, INC) containing B. subtilis strain QST713 as the active ingredient. In addition to application alone, the B. amyloliquefaciens RTI 472 strain is also used in a regimen for preventing/treating phytopathogenic infections in which it is applied to plants at alternate time intervals with one or more commercially available chemical fungicides/bactericides. In some cases, the efficacy of the application of the B. amyloliquefaciens RTI 472 strain alone is as good as the chemical fungicide/bactericide. In some cases, the RTI 472 strain can be used in one of the chemically active agents in the alternative. The RTI 472 strain is also effective in increasing yield and reducing disease when used as a corn seed treatment agent. Experimental results provide new RTI472 strain enhanced FRACTURE (CONSUMO EM VERDE (CEV), BIOTECNOLOGIA DAS PLANTAS An example of the benefit of the antagonistic properties of S.A., PORTUGAL), FRACTURE is a plant extract containing a BLAD polypeptide as an active ingredient. Identification of new antimicrobial metabolites produced by the RTI 472 strain is also described.

在本發明之一個實施例中，提供一種包括以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物的組合物，該組合物用於施用於植物以達成使植物生長獲益或對易感病植物賦予保護以避免病原性感染中的一或兩者。 In one embodiment of the present invention, there is provided a composition comprising a biologically pure culture of Bacillus amyloliquefaciens RTI472 deposited with ATCC PTA-121166 or a mutant having all of its identifying characteristics, the composition being used in Application to plants to achieve one or both of the benefits of plant growth or protection of susceptible plants to avoid pathogenic infections.

在另一個實施例中，提供一種用於達成使植物生長獲益或對易感病植物賦予保護以避免病原性感染中之一或兩者的方法，該方法包括將包含以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物的組合物輸送至：植物葉、植物莖皮、植物果實、植物花、植物種子、植物根部、植物剪枝、植物移植物、植物癒合組織；植物周圍土壤或生長介質；在土壤或生長介質中播種植物種子之前的土壤或生長介質；或在土壤或生長介質中種植植物、植物剪枝、植物移植物或植物癒合組織之前的土壤或生長介質，該組合物之呈適合於使植物生長獲益及/或對易感病植物賦予保護以避免病原性感染之量。 In another embodiment, a method for achieving one or both of plant growth benefit or protection of a susceptible plant to avoid pathogenic infection is provided, the method comprising comprising an ATCC PTA-121166 A composition of the deposited biologically pure culture of Bacillus amyloliquefaciens RTI472 or a mutant thereof having all of its identifying characteristics is delivered to: plant leaves, plant bark, plant fruits, plant flowers, plant seeds, plant roots, plant shears Branches, plant grafts, plant healing tissues; soils or growth media surrounding plants; soils or growth media prior to planting plant seeds in soil or growth media; or planting plants, plant pruning, plant grafts in soil or growth media Or the soil or growth medium prior to the healing of the tissue, the composition being present in an amount suitable to benefit plant growth and/or to confer protection to susceptible plants to avoid pathogenic infections.

已知有益的植物相關細菌(根圍及內寄生)對宿主植物提供範圍為抗疾病及昆蟲蟲害及耐受環境壓力(包括冷、鹽度及乾旱壓力)之多種益處。由於接種植物促生長細菌之植物自土壤獲取更多水及養分，例如歸因於較佳發育之根系統，所以植物更健康生長且較不易受生物及非生物壓力影響。因此，本發明之微生物組合物可單獨或與當前作物管理輸入(諸如化學肥料、除草劑及殺蟲劑)組合施用以使作物產率最大化。促進植物生長影響轉變為植物生長較快及地上生物質量增加，該特性可應用於提高早期活力。提高早期活力之一個益處為植物更具競爭性且勝過雜草，藉由使勞動力及除草劑施用最小化直接降低雜草 管理成本。促進植物生長影響亦轉變為改善之根發育，包括更深及更寬之根，有更多細根參與水及養分之吸收。此特性允許更佳地使用農業資源，且減少用於灌溉需求及/或施肥之水。根發育及根結構之變化影響植物與其他土壤媒介微生物之相互作用，該等微生物包括有助於植物吸收養分(包括固氮及磷酸鹽溶解)之有益真菌及細菌。此等有益微生物亦與植物病原體競爭，提高總體植物健康且減少對化學殺真菌劑及殺蟲劑的需求。 Beneficial plant-associated bacteria (root and endoparasite) are known to provide host plants with a variety of benefits ranging from disease and insect pests to environmental stresses including cold, salinity and drought stress. Since plants inoculated with plant growth-promoting bacteria obtain more water and nutrients from the soil, for example due to better developed root systems, plants grow healthier and less susceptible to biotic and abiotic stresses. Thus, the microbial compositions of the present invention can be applied alone or in combination with current crop management inputs, such as chemical fertilizers, herbicides, and insecticides, to maximize crop yield. Promoting the effects of plant growth translates into faster plant growth and increased above-ground biomass quality, which can be applied to improve early vigor. One of the benefits of improving early vigor is that plants are more competitive and outweigh weeds, directly reducing weeds by minimizing labor and herbicide application. Management costs. The promotion of plant growth effects is also transformed into improved root development, including deeper and wider roots, with more fine roots involved in the absorption of water and nutrients. This feature allows for better use of agricultural resources and reduces water for irrigation needs and/or fertilization. Root development and changes in root structure affect the interaction of plants with other soil-borne micro-organisms, including beneficial fungi and bacteria that help plants absorb nutrients, including nitrogen fixation and phosphate dissolution. These beneficial microorganisms also compete with plant pathogens, improving overall plant health and reducing the need for chemical fungicides and pesticides.

盤分析中解澱粉芽孢桿菌RTI472對抗若干主要植物病原體之拮抗特性描述於實例3中且諸如乙偶姻及吲哚乙酸(IAA)之植物激素產生的表現性狀及菌株之養分循環描述於實例4中。另外，在溫室中及田間試驗中對各種作物進行研究以確定解澱粉芽孢桿菌RTI472菌株預防及/或減輕許多常見植物病原體對植物之天然或人工感染作用的能力。結果描述於實例5至14及圖3至14中。 The antagonistic properties of B. amyloliquefaciens RTI472 against several major plant pathogens in the disc assay are described in Example 3 and the performance traits of phytohormones such as acetoin and indole acetic acid (IAA) and the nutrient cycling of the strain are described in Example 4. . In addition, various crops were studied in greenhouse and in field trials to determine the ability of B. amyloliquefaciens RTI 472 strain to prevent and/or alleviate the natural or artificial infection effects of many common plant pathogens on plants. The results are described in Examples 5 to 14 and Figures 3 to 14.

實例5描述解澱粉芽孢桿菌RTI472菌株減輕植物病原體豆銹病(菜豆銹病菌)及植物病原體胡椒葡萄孢枯萎病(灰葡萄孢菌)之作用的能力。另外，在第一組實驗中，針對RTI472菌株施用於葉面控制菜豆銹病菌及灰葡萄孢菌，測試解澱粉芽孢桿菌RTI472菌株之不同調配物以及另一及新鑑別之解澱粉芽孢桿菌菌株RTI301。實驗設計經設定，以在感染病原體九天後，評估以下各者之疾病控制%：單獨用水稀釋之廢醱酵液中RTI472孢子(「RTI472+1%SFB」)、用水稀釋之廢醱酵液中RTI472孢子加上酵母提取物(「RTI472+1%SFB+酵母提取物」)、單獨用水稀釋100倍之廢醱酵液中RTI301孢子(「RTI301+1%SFB」)、用水稀釋100倍之廢醱酵液中RTI301孢子加上酵母提取物(「RTI301+1%SFB+酵母提取物」)、BRAVO WEATHER STIK(500g a.i./ha四氯異苯腈),HORIZON(50g a.i./ha得克利)及與RTI472及RTI301菌株相同孢子濃度之SERENADE OPTIMUM。未經處理之對照 (僅水)產生28%疾病。豆銹病及胡椒葡萄孢枯萎病實驗之結果類似。豆銹病實驗之結果展示在表III中且指示與未添加酵母提取物而施用之菌株相比，添加酵母提取物使得疾病控制約增加40%。當以相同施用率(亦即1×108cfu/ml)施用時，RTI472+1%SFB+酵母提取物及RTI301+1%SFB+酵母提取物展現之疾病控制量類似於針對SERENADE OPTIMUM所觀測之結果，即使RTI472及RTI301調配物中SFB之量相對較低，為1%，且SFB可預期含有具有抗真菌活性之分泌化合物。另一關於RTI472菌株對豆銹病之疾病控制的類似實驗描述於實例6中，且結果展示在表IV及圖3中。結果展示與用SERENADE OPTIMUM處理相比，當以相同施用率施用時，用RTI472孢子處理豆類植物葉後豆銹病(菜豆銹病菌)之控制相當。 Example 5 describes the ability of the B. amyloliquefaciens RTI 472 strain to alleviate the effects of plant pathogen bean rust (Nicky rust fungus) and the plant pathogen Botrytis cinerea wilt (Botrytis cinerea). In addition, in the first set of experiments, the RTI472 strain was applied to the leaf control bean rust fungus and Botrytis cinerea, the different formulations of the B. amyloliquefaciens RTI472 strain and another and newly identified B. amyloliquefaciens strain RTI301 were tested. . The experimental design was set up to assess the disease control % of the following individuals after nine days of infection with the pathogen: RTI472 spores ("RTI472+1%SFB") in the waste broth diluted with water alone, in the waste broth diluted with water RTI472 spore plus yeast extract ("RTI472+1%SFB+yeast extract"), RTI301 spores ("RTI301+1%SFB") in 100% of the waste broth diluted with water, diluted 100 times with water RTI301 spore plus yeast extract ("RTI301+1%SFB+yeast extract"), BRAVO WEATHER STIK (500g ai/ha tetrachloroisophthalonitrile), HORIZON (50g ai/ha Dekli) and RTI472 SERENADE OPTIMUM with the same spore concentration of RTI301 strain. An untreated control (water only) produced 28% of the disease. The results of the bean rust and Botrytis cinerea wilt disease experiments were similar. The results of the bean rust test are shown in Table III and indicate that the addition of the yeast extract resulted in an approximately 40% increase in disease control compared to the strain administered without the addition of the yeast extract. The RTI472+1%SFB+ yeast extract and RTI301+1%SFB+ yeast extract exhibited disease control levels similar to those observed for SERENADE OPTIMUM when administered at the same application rate (i.e., 1 x 10 8 cfu/ml). Even though the amount of SFB in the RTI 472 and RTI 301 formulations is relatively low, it is 1%, and SFB is expected to contain a secretory compound having antifungal activity. Another similar experiment on disease control of RTI 472 strain against bean rust is described in Example 6, and the results are shown in Table IV and Figure 3. The results show that the control of bean rust (leaf bean rust) with RTI472 spores is comparable to that treated with SERENADE OPTIMUM when applied at the same application rate.

實例6及表V及圖4中之結果展示與用SERENADE OPTIMUM處理相比，當以相同施用率施用時，用RTI472孢子處理大豆葉後大豆白粉病(白粉病菌)之控制得到改善。 The results in Example 6 and Table V and Figure 4 show that the control of soybean powdery mildew (Saccharomyces cerevisiae) after treatment of soybean leaves with RTI472 spores was improved when applied at the same application rate compared to treatment with SERENADE OPTIMUM.

實例7描述一個類似實驗，其展示RTI472菌株能夠控制灰葡萄孢菌所引起之胡椒葡萄孢枯萎病。結果展示在表VI及圖5中且展示與用SERENADE OPTIMUM處理葉相比，在同等濃度細菌孢子下，用RTI472孢子處理胡椒植物葉後病原體控制得到改善。 Example 7 describes a similar experiment showing that the RTI 472 strain is capable of controlling the Botrytis cinerea wilt caused by Botrytis cinerea. The results are shown in Table VI and Figure 5 and show that pathogen control is improved after treatment of pepper plant leaves with RTI 472 spores under the same concentration of bacterial spores compared to leaves treated with SERENADE OPTIMUM.

其他實驗描述在實例7中，展示與SERENADE OPTIMUM相比，胡椒葡萄孢枯萎病(灰葡萄孢菌)之控制得到改善。結果展示在表VII及圖6中且展示與用SERENADE OPTIMUM處理葉相比，在同等濃度細菌孢子下，用增加濃度之RTI472孢子處理胡椒植物葉改善病原體控制。 Other Experimental Descriptions In Example 7, the control of Botrytis cinerea wilt (Botrytis cinerea) was improved compared to SERENADE OPTIMUM. The results are shown in Tables VII and 6 and show that treatment of pepper plant leaves with increasing concentrations of RTI472 spores improves pathogen control under the same concentration of bacterial spores compared to leaves treated with SERENADE OPTIMUM.

進一步結果描述於實例7、表VIII及圖7至12中，展示與用產品SERENADE OPTIMUM處理相比，在同等濃度細菌孢子下，在用病原體(50k、100k、500k、1M及2M分生孢子/毫升)增加劑量接種植物之 後用RTI472孢子處理胡椒植物葉控制病原體胡椒葡萄孢枯萎病(灰葡萄孢菌)。圖8至12為在分別感染增加劑量之胡椒葡萄孢枯萎病50k、100k、500k、1M及2M分生孢子/毫升4天後及用RTI472或SERENADE OPTIMUM孢子處理胡椒植物葉後胡椒植物之影像。在3個最低病原體接種率下，觀測到RTI472菌株及SERENADE OPTIMUM之疾病控制百分比類似。然而，觀測到與SERENADE OPTIMUM相比，在2個最高病原體接種率1M及2M下，經RTI472菌株處理之植物達到統計學改善。 Further results are described in Example 7, Table VIII and Figures 7 to 12, showing the use of pathogens (50k, 100k, 500k, 1M and 2M conidia/ under the same concentration of bacterial spores compared to the product SERENADE OPTIMUM treatment) ML) increase the dose of inoculated plants The pepper plant leaves were treated with RTI472 spores to control the pathogen Botrytis cinerea (B. cinerea). Figures 8 to 12 are images of pepper plants after treatment with increasing doses of Botrytis cinerea wilt, 50k, 100k, 500k, 1M and 2M conidia/ml for 4 days and with RTI472 or SERENADE OPTIMUM spores. At the three lowest pathogen inoculation rates, the percentage of disease control observed for RTI 472 strain and SERENADE OPTIMUM was similar. However, it was observed that plants treated with the RTI 472 strain achieved statistical improvement at the highest vaccination rates of 1 M and 2 M compared to SERENADE OPTIMUM.

在田間試驗中對各種作物進行研究以確定解澱粉芽孢桿菌RTI472菌株預防及/或減輕許多常見植物病原體對植物之天然或人工感染作用的能力。結果描述於實例8至11。在試驗中，RTI472以與SERENADE OPTIMUM相同之施用率施用於作物葉。RTI472孢子作為單獨生物殺真菌劑或與市售化學殺真菌劑/殺菌劑之組合施用於植物。施用1至6次，其中施用之間的時間間隔為5至7天，視作物而定。第一次施用之時間安排視特定作物而定且在種植時、作物萌芽後幾週、開花時、出現疾病時或在預期疾病出現前的範圍內。 Various crops were studied in field trials to determine the ability of B. amyloliquefaciens RTI 472 strain to prevent and/or alleviate the natural or artificial infection effects of many common plant pathogens on plants. The results are described in Examples 8 to 11. In the test, RTI 472 was applied to the crop leaves at the same application rate as SERENADE OPTIMUM. The RTI 472 spores are applied to the plants as a separate biofungicide or in combination with a commercially available chemical fungicide/bactericide. The application is 1 to 6 times, wherein the time interval between administrations is 5 to 7 days, depending on the crop. The timing of the first application depends on the particular crop and is within the range of the crop, the weeks after the crop germination, the flowering, the onset of the disease, or before the onset of the expected disease.

實例8及表IX-XI中之結果展示與SERENADE OPTIMUM相比，當以相同施用率施用時，作為單獨生物殺真菌劑或作為使用含有化學殺真菌劑/殺菌劑之商業產品的處理方案之一部分，RTI472對葫蘆中之白粉病的控制類似或改善。作為單獨生物殺真菌劑之RTI472展示與使用行業標準氟吡菌醯胺(Fluopyram)加上得克利殺真菌劑之方案類似的效能。 The results in Example 8 and Tables IX-XI show a portion of the treatment scheme as a separate biofungicide or as a commercial product containing a chemical fungicide/bactericide when applied at the same application rate compared to SERENADE OPTIMUM. , RTI472 similar or improved control of powdery mildew in gourd. RTI 472, a separate biofungicide, demonstrates efficacy similar to the use of the industry standard Fluopyram plus Dickle fungicide.

實例9及表XII-XIV中之結果展示與SERENADE OPTIMUM相比，當以相同施用率施用時，作為單獨生物殺真菌劑或作為使用含有化學殺真菌劑/殺菌劑之商業產品的處理方案之一部分，RTI472對食莢菜豆中白黴之控制類似或改善。作為單獨生物殺真菌劑之RTI472展示與 使用行業標準氟吡菌醯胺加上得克利殺真菌劑之方案類似的效能。 The results in Example 9 and Tables XII-XIV show a portion of the treatment scheme as a separate biofungicide or as a commercial product containing a chemical fungicide/bactericide when applied at the same application rate compared to SERENADE OPTIMUM. , RTI472 similar or improved control of white mold in pod bean. RTI472 display as a separate biofungicide Similar efficacy was achieved using the industry standard flupirtine plus the Dickle fungicide.

實例9及表XII中之結果展示與SERENADE OPTIMUM相比，當以相同施用率施用時，作為單獨生物殺真菌劑或作為使用含有化學殺真菌劑/殺菌劑之商業產品的處理方案之一部分，RTI472對食莢菜豆中之白黴的控制類似或改善。觀測到使用解澱粉芽孢桿菌RTI472與甲基托布津(Thiophanate-methyl)殺真菌劑之組合的方案對食莢菜豆之白黴控制最佳，其甚至比基於使用丙硫菌唑(Prothioconazole)殺真菌劑與甲基托布津殺真菌劑組合之化學方案更佳。 The results in Example 9 and Table XII show that RTI 472 is part of a single biofungicide or as part of a treatment scheme using a commercial product containing a chemical fungicide/bactericide when applied at the same application rate compared to SERENADE OPTIMUM. The control of the white mold in the pod bean is similar or improved. It was observed that the combination of B. amyloliquefaciens RTI472 and Thiophanate-methyl fungicides was the best control for the white mold of the white bean, which was even more fungi-based than the use of prothioconazole. The chemical combination of the agent and the methyl thiophanate fungicide is better.

實例9及表XIII-XIV中之結果展示與SERENADE OPTIMUM相比，當以相同施用率施用時，作為單獨生物殺真菌劑，RTI472對花生中葉斑病及南方白黴菌之控制改善且產量顯著增加(表XIII中之中壓及表XIV中之重壓)。 The results in Example 9 and Tables XIII-XIV show that compared to SERENADE OPTIMUM, when applied at the same application rate, as a separate biofungicide, RTI 472 has improved control of leaf spot and P. sinensis in peanuts and a significant increase in yield ( The intermediate pressure in Table XIII and the weight in Table XIV).

實例10及表XV中之結果展示與SERENADE OPTIMUM相比，當以相同施用率施用時，作為單獨生物殺真菌劑，RTI472對小麥赤黴病及大豆銹病之控制改善。實例10及表XV中之結果展示與對照植物相比，當作為單獨生物殺真菌劑施用時RTI472對番茄中立枯交鏈孢菌(Alternaria solani)之控制改善。 The results in Example 10 and Table XV show that RTI 472 has improved control of wheat scab and soybean rust as a separate biofungicide when applied at the same application rate compared to SERENADE OPTIMUM. The results in Example 10 and Table XV show an improvement in the control of RTI472 against Alternaria solani when applied as a separate biofungicide compared to control plants.

實例11及表XVI中之結果展示與SERENADE OPTIMUM相比，當以相同施用率施用時，作為單獨生物殺真菌劑或作為使用含有化學殺真菌劑/殺菌劑之商業產品的處理方案之一部分，RTI472對蕃茄中之番茄細菌性斑病(黃單孢菌屬)的控制改善。觀測到解澱粉芽孢桿菌RTI472單獨或在與氫氧化銅之方案中對蕃茄中番茄細菌性斑病(黃單孢菌屬)之控制最佳，且勝過基於使用四氯異苯腈與氫氧化銅組合之方案。 The results in Example 11 and Table XVI show RTI472 as part of a single biofungicide or as part of a treatment scheme using a commercial product containing a chemical fungicide/fungicide when applied at the same application rate compared to SERENADE OPTIMUM. Improved control of tomato bacterial spot disease (Homomonospora) in tomatoes. It was observed that B. amyloliquefaciens RTI472 was the best control of tomato bacterial spot disease (Homomonospora) in tomato alone or in a copper hydroxide scheme, and was superior to the use of tetrachloroisophthalonitrile and hydroxide. The combination of copper.

實例12描述除化學活性劑之典型組合(MAXIM+滅達樂(Metalaxyl)+PONCHO 250)之外，用解澱粉芽孢桿菌RTI472菌株之孢 子塗佈玉米種子之有益作用，以提高產量且控制天然存在之植物疾病以及絲核菌及禾穀鐮刀菌感染。在第一個實驗中，未經處理之種子及各經處理之玉米種子種植在威斯康星州(Wisconsin)之三個分開田間試驗中，且藉由達至植物萌芽之時間長度、植株密度、植物活力及籽粒產量(以蒲式耳/英畝計)分析。與經單獨化學對照處理之種子相比，種子處理劑中包括解澱粉芽孢桿菌RTI472對達至植物萌芽之時間、植株密度或植物活力無統計學上顯著之作用，但引起籽粒6蒲式耳/英畝之增加(231至237蒲式耳/英畝)，表示籽粒產量增加2.6%。在一個相關實驗中，當試驗中之玉米植物分開用病原體絲核菌及禾穀鐮刀菌攻擊時，與經單獨化學對照處理之種子相比，用解澱粉芽孢桿菌RTI472處理種子使絲核菌與天然存在之疾病的疾病嚴重程度達統計學上顯著之降低。 Example 12 describes the use of a strain of Bacillus amyloliquefaciens RTI472 in addition to a typical combination of chemically active agents (MAXIM + Metalaxyl + PONCHO 250) The beneficial effect of sub-coating corn seeds to increase yield and control naturally occurring plant diseases as well as Rhizoctonia and Fusarium graminearum infections. In the first experiment, untreated seeds and treated corn seeds were planted in three separate field trials in Wisconsin, and by the length of plant germination, plant density, plant vigor And grain yield (in bushels per acre) analysis. Compared with the seeds treated with the chemical control alone, the B. amyloliquefaciens RTI472 included in the seed treatment agent had no statistically significant effect on the time to plant germination, plant density or plant vigor, but caused 6 bushels per acre of grain. An increase (231 to 237 bushels per acre) indicates a 2.6% increase in grain production. In a related experiment, when the corn plants in the experiment were challenged separately by the pathogenic Rhizoctonia and Fusarium graminearum, the seeds were treated with Bacillus amyloliquefaciens RTI472 to make Rhizoctonia solani and the seeds treated with the chemical control alone. The severity of the disease in naturally occurring diseases is statistically significantly reduced.

實例13描述在草莓田間試驗中進行之研究以確定解澱粉芽孢桿菌RTI472菌株預防及/或減輕植物病原體褐灰黴(灰葡萄孢菌)作用的能力。RTI472菌株與施用稱為「FARMER方案」之化學活性劑組合及施用枯草芽孢桿菌菌株QST713之濃度比RTI472菌株高10倍的SERENADE MAX比較。表XVIII中之結果展示觀測到與未經處理之對照相比，所有三個處理解澱粉芽孢桿菌RTI472、SERENADE MAX及FARMER方案均改善對草莓上褐灰黴之控制，其中用RTI472處理之產量增加數值略高。各草莓試驗中未經處理之對照(「UT」)中感染灰葡萄孢菌病原體之果實隨時間推移之發育展示在圖13中之圖中。 Example 13 describes a study conducted in a strawberry field trial to determine the ability of B. amyloliquefaciens RTI 472 strain to prevent and/or alleviate the effects of the plant pathogen, Botrytis cinerea (B. cinerea). The RTI 472 strain was compared with the application of a chemical active agent called "FARMER protocol" and the application of B. subtilis strain QST713 at a concentration 10 times higher than the RTI 472 strain of SERENADE MAX. The results in Table XVIII show that all three treatments of B. amyloliquefaciens RTI472, SERENADE MAX, and FARMER protocols improved control of B. cinerea on strawberries compared to untreated controls, with increased yields treated with RTI472 The value is slightly higher. The development of the fruit of the infected Botrytis cinerea pathogen in the untreated control ("UT") in each strawberry test over time is shown in the graph of Figure 13.

實例14描述夏南瓜之田間試驗研究以確定解澱粉芽孢桿菌RTI472菌株單獨或作為與活性劑之混合物在產品FRACTURE中預防及/或減輕由高氏白粉病菌(Golovinomyces cichoracearum，ERYSCI)所引起之白粉病作用的能力。實驗結果展示於表XIX中。FRACTURE提供疾病嚴重程度大約15%減少，而SERENADE OPTIMUM及RTI472得到 疾病嚴重程度大約30%減少。觀測到BRAVO WEATHER STIK(45%)及LUNA EXPERIENCE(94%)之疾病嚴重程度減少最顯著。當RTI472及FRACTURE一起施用時，觀測到疾病控制之累積作用，引起疾病嚴重程度減少47%。此與BRAVO WEATHER STIK疾病嚴重程度之減少(45%)相當，且證實解澱粉芽孢桿菌RTI472與FRACTURE之組合作為控制葫蘆上白粉病之方案中四氯異苯腈殺真菌劑的替代物的適用性。 Example 14 describes a field trial of zucchini to determine whether B. amyloliquefaciens RTI 472 strain alone or as a mixture with an active agent prevents and/or alleviates powdery mildew caused by Golovinomyces cichoracearum (ERYSCI) in product FRACTURE The ability to function. The experimental results are shown in Table XIX. FRACTURE provides approximately a 15% reduction in disease severity, while SERENADE OPTIMUM and RTI472 have a reduction in disease severity of approximately 30%. The reduction in disease severity was most pronounced in BRAVO WEATHER STIK (45%) and LUNA EXPERIENCE (94%). When RTI 472 and FRACTURE were administered together, a cumulative effect of disease control was observed, resulting in a 47% reduction in disease severity. This is comparable to the reduction in the severity of BRAVO WEATHER STIK disease (45%) and confirms the suitability of the combination of B. amyloliquefaciens RTI472 and FRACTURE as an alternative to the tetrachloroisophthalonitrile fungicide in the regimen for controlling powdery mildew on cucurbits. .

實例15描述由解澱粉芽孢桿菌RTI472菌株產生之環狀脂肽豐原素及去羥基豐原素之研究，且意料地，先前未報導之幾類此等分子的鑑別。確定解澱粉芽孢桿菌RTI472產生先前報導之豐原素A、豐原素B及豐原素C化合物以及去羥基豐原素A、去羥基豐原素B及去羥基豐原素C化合物。意外地，除此等已知之化合物之外，確定RTI472菌株亦產生先前未鑑別之此等化合物之衍生物，其中環狀肽鏈之位置8的L-異白胺酸(圖14中稱為X3)經L-甲硫胺酸置換。新類別豐原素及去羥基豐原素在本文中稱為MA、MB及MC，係指A類、B類及C類衍生物，其中圖14中X3處之L-異白胺酸經L-甲硫胺酸置換。新鑑別之分子粗體展示在圖14中及表XX中。如表XX所述，在RTI472菌株之培養物中未觀測到去羥基豐原素MC化合物。進一步確定RTI472菌株產生先前尚未鑑別之其他類別豐原素。在此類別中，豐原素B之L-異白胺酸(圖14中之位置X3)經L-高半胱胺酸(Hcy)置換。此先前未鑑別之豐原素代謝物在本文中稱作豐原素H且粗體展示在圖14及表XX中。進一步確定RTI472菌株產生先前未鑑別之其他類別豐原素及去羥基豐原素代謝物。在此類別中，環狀肽主鏈結構之位置4(圖14中之位置X1)的胺基酸經L-異白胺酸置換。此等先前未鑑別之代謝物在本文中稱為豐原素I及去羥基豐原素I且展示在圖14及表XX中。 Example 15 describes the study of the cyclic lipopeptides auretolide and dehydroxylectin produced by the B. amyloliquefaciens RTI 472 strain, and unexpectedly, the identification of several classes of molecules not previously reported. It was determined that B. amyloliquefaciens RTI472 produced previously reported protosulin A, abundance B and abundance C compounds as well as dehydroxylectin A, dehydroxylectin B and dehydroxylectin C compounds. Surprisingly, in addition to such known compounds, it was determined that the RTI 472 strain also produced derivatives of such compounds that were not previously identified, wherein the L-isoleucine at position 8 of the cyclic peptide chain (referred to as X in Figure 14) 3 ) Replacement with L-methionine. The new classes of protosan and dehydroxylectin are referred to herein as MA, MB and MC, and refer to class A, B and C derivatives, wherein L-isoleucine at X 3 in Figure 14 is L- Methionine replacement. The newly identified molecular bold is shown in Figure 14 and in Table XX. As shown in Table XX, no dehydroxylectin MC compound was observed in the culture of the RTI472 strain. It was further determined that the RTI 472 strain produced other classes of amylin which had not been previously identified. In this category, L- fengycin B is isoleucine (FIG. 14 in the position X 3) via L- homocysteine (of Hcy) replacement. This previously unidentified abundane metabolite is referred to herein as a protocin H and is shown in bold in Figure 14 and Table XX. It was further determined that the RTI 472 strain produced other classes of abundance and dehydroxylectin metabolites that were not previously identified. In this category, the position of the main chain structure of the cyclic peptide 4 (FIG. 14 in the position X 1) is replaced by the amino acid L- isoleucine. These previously unidentified metabolites are referred to herein as protamine I and dehydroxylectin I and are shown in Figure 14 and Table XX.

實例16描述拮抗脂肽自解澱粉芽孢桿菌菌株RTI472廢醱酵液之分離及活體外盤分析，展示分離之脂肽保留其對抗兩種常見植物病原 體之活性。培養RTI472且藉由LCMS分析培養物上清液之酸沈澱以比較伊枯草菌素、表面活性素及豐原素之相對豐度。圖15為展示在酸沈澱之後自RTI472廢醱酵液回收之脂肽的百分比之圖。結果展示脂肽總量之83%藉由酸沈澱回收。隨後，針對灰葡萄孢菌及禾穀鐮刀菌，用藉由LCMS分析之相同樣品進行盤生物分析。結果展示在圖16A-16D中且展示酸沈澱之樣品與起始廢醱酵液具有類似程度之對抗灰葡萄孢菌與禾穀鐮刀菌之拮抗活性。 Example 16 describes the isolation of an antagonistic lipopeptide from B. amyloliquefaciens strain RTI 472 and an in vitro disk assay showing that the isolated lipopeptide retains against two common plant pathogens. Body activity. RTI 472 was cultured and the acid precipitate of the culture supernatant was analyzed by LCMS to compare the relative abundances of italin, surfactin and abundance. Figure 15 is a graph showing the percentage of lipopeptide recovered from the RTI 472 waste mash after acid precipitation. As a result, 83% of the total amount of lipopeptide was recovered by acid precipitation. Subsequently, for the Botrytis cinerea and Fusarium graminearum, disc bioassay was performed using the same sample analyzed by LCMS. The results are shown in Figures 16A-16D and show that the acid precipitated sample has a similar degree of antagonistic activity against Botrytis cinerea and Fusarium graminearum as the starting waste mash.

在一個實施例中，提供一種包括以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物的組合物，該組合物用於施用於植物以達成使植物生長獲益或對易感病植物賦予保護以避免病原性感染中的一或兩者。植物生長益處及/或所賦予之保護可藉由幼苗活力提高、根發育改善、植物生長改善、植物健康提高、產量增加、外觀改善、對植物病原體之抗性提高、病原性感染減少或其組合展現。 In one embodiment, there is provided a composition comprising a biologically pure culture of B. amyloliquefaci RTI472 or a mutant having all of its identifying characteristics deposited under ATCC PTA-121166, for use in application to a plant To achieve benefits from plant growth or to confer protection to susceptible plants to avoid one or both of the pathogenic infections. Plant growth benefits and/or protection afforded by increased seedling vigor, improved root development, improved plant growth, improved plant health, increased yield, improved appearance, increased resistance to plant pathogens, reduced pathogenic infection, or combinations thereof Show.

本發明之組合物及方法有益於各種植物，包括(但不限於)單子葉植物、雙子葉植物、穀物、玉米、甜玉米、爆玉米、玉米種、青貯玉米、飼料玉米、稻穀、小麥、大麥、高粱、蘆筍、漿果、藍莓、黑莓、樹莓、羅甘莓、越橘、蔓越橘、醋栗、接骨木、黑醋栗、蔓越莓、越莓、甘藍型蔬菜、椰菜、甘藍菜、花椰菜、芽甘藍、散葉甘蘭、羽衣甘藍、芥菜、球莖甘藍、瓜類蔬菜、黃瓜、哈密瓜、甜瓜、香甘瓜、番南瓜、西瓜、南瓜、茄子、鱗莖類蔬菜、洋蔥、大蒜、蔥、陳皮、橘子、葡萄柚、檸檬、紅橘、橘柚、柚、果蔬菜、胡椒、番茄、地面櫻桃、樹蕃茄、秋葵、葡萄、香草/香料、綠葉蔬菜、生菜、芹菜、菠菜、香芹、菊苣、豆科植物/蔬菜(多汁且乾燥之豆子及豌豆)、豆子、四季豆、食莢菜豆、殼豆、大豆、乾豆、回回豆、利馬豆、豌豆、鷹嘴豆、豌豆瓣、扁豆、油料種子作物、芥花、菌麻、 椰子、棉花、亞麻、油棕櫚、橄欖、花生、油菜籽、紅花、芝麻、向日葵、大豆、梨果、蘋果、海棠果、梨、木瓜、夏花山楂、根/塊莖及球莖蔬菜、胡蘿蔔、馬鈴薯、甘薯、木薯、甜菜、薑、辣根、蘿蔔、人參、蕪菁、核果、杏、櫻桃、油桃、桃子、李子、梅子、草莓、堅果、杏仁、開心果、山核桃、核桃、榛子、板栗、腰果、山毛櫸堅果、胡桃、夏威夷果、獼猴桃、香蕉、(藍)龍舌蘭、草、草皮草、觀賞植物、一品紅、繡球花、硬枝地插、栗子、橡木、楓木、甘蔗及甜菜。 The compositions and methods of the present invention are beneficial to a variety of plants including, but not limited to, monocots, dicots, grains, corn, sweet corn, popcorn, corn, silage corn, feed corn, rice, wheat, barley , sorghum, asparagus, berries, blueberries, blackberries, raspberries, raspberries, cranberries, cranberries, gooseberries, elderberries, currants, cranberries, berry, cabbage, broccoli, kale Dish, broccoli, Brussels sprouts, broccoli, kale, mustard, broccoli, melon, cucumber, cantaloupe, melon, cantaloupe, squash, watermelon, pumpkin, eggplant, bulbous vegetables, onions, garlic, onions , tangerine peel, orange, grapefruit, lemon, red tangerine, tangerine, pomelo, fruit and vegetable, pepper, tomato, ground cherry, tree tomato, okra, grape, vanilla/spice, green leafy vegetables, lettuce, celery, spinach, fragrant Parsley, chicory, legumes/vegetables (succulent and dry beans and peas), beans, green beans, food pods, shell beans, soybeans, dried beans, peas, lima beans, peas, chickpeas, peas Petals, lentil, oilseed crop, canola, flax bacteria, Coconut, cotton, flax, oil palm, olive, peanut, rapeseed, safflower, sesame, sunflower, soybean, pear, apple, jellyfish, pear, papaya, summer flower hawthorn, root/tuber and bulbous vegetables, carrot, potato Sweet potato, cassava, beet, ginger, horseradish, radish, ginseng, turnip, stone fruit, apricot, cherry, nectarine, peach, plum, plum, strawberry, nut, almond, pistachio, pecan, walnut, hazelnut, chestnut , cashew nuts, beech nuts, walnuts, macadamia nuts, kiwis, bananas, (blue) agave, grass, turfgrass, ornamental plants, poinsettia, hydrangea, hardwoods, chestnuts, oak, maple, sugar cane and beets .

在一或多個實施例中，植物可包括大豆、豆子、食莢菜豆、小麥、棉花、玉米、胡椒、番茄、馬鈴薯、木薯、葡萄、草莓、香蕉、花生、番南瓜、南瓜、茄子及黃瓜。 In one or more embodiments, the plant may include soybean, bean, pod bean, wheat, cotton, corn, pepper, tomato, potato, tapioca, grape, strawberry, banana, peanut, squash, pumpkin, eggplant, and cucumber. .

在本發明之組合物及方法中，病原性感染可由各種植物病原體引起，該等植物病原體包括例如(但不限於)植物真菌病原體、植物細菌病原體、鏽菌、葡萄孢屬、灰葡萄孢菌、蔥鱗葡萄孢菌(Botrytis squamosa)、歐文菌屬、胡蘿蔔軟腐歐文菌(Erwinia carotovora)、解澱粉歐文菌(Erwinia amylovora)、狄克氏菌屬(Dickeya spp.)、達旦狄克氏菌(Dickeya dadantii)、黑脛病菌(Dickeya solani)、土壤桿菌屬(Agrobacterium spp.)、根癌土壤桿菌(Agrobacterium tumefaciens)、黃單孢菌屬、地毯草黃單孢菌(Xanthomonas axonopodis)、油菜黃單孢菌胡蘿蔔致病變種(Xanthomonas campestris pv.carotae)、桃李黃單孢菌(Xanthomonas pruni)、核桃黃單孢菌(Xanthomonas arboricola)、水稻黃單孢菌水稻變種(Xanthomonas oryzae pv.oryzae)、焦枯菌屬(Xylella spp.)、葉緣焦枯菌(Xylella fastidiosa)、卡地菌屬(Candidatus spp.)、韌皮部桿菌(Candidatus liberibacter)、鐮刀菌屬、大刀鐮刀菌(Fusarium colmorum)、禾穀鐮刀菌、尖孢鐮刀菌、古巴尖孢鐮刀菌(Fusarium oxysporum f.sp.Cubense)、番茄尖孢鐮刀菌(Fusarium oxysporum f.sp.Lycopersici)、棒形鐮刀菌(Fusarium virguliforme)、核盤菌屬(Sclerotinia spp.)、向日葵核盤菌(Sclerotinia sclerotiorum)、小核盤菌(Sclerotinia minor)、同果核盤菌(Sclerotinia homeocarpa)、尾孢菌屬(Cercospora/Cercosporidium spp.)、鉤絲殼屬(Uncinula spp.)、葡萄白粉病菌(Uncinula necator)(白粉病(Powdery Mildew))、叉絲單囊殼屬(Podosphaera spp.)(白粉病)、白叉絲單囊殼(Podosphaera leucotricha)、克蘭德叉絲單囊殼(Podosphaera clandestine)、擬莖點菌屬(Phomopsis spp.)、葡萄擬莖點黴病菌(Phomopsis viticola)、交鏈孢菌屬(Alternaria spp.)、細極鏈格孢菌(Alternaria tenuissima)、蔥鏈格孢菌(Alternaria porri)、交鏈格孢菌(Alternaria alternate)、立枯交鏈孢菌、鏈格孢菌(Alternaria tenuis)、假單胞菌屬、丁香假單胞菌番茄變種(Pseudomonas syringae pv.Tomato)、疫黴菌屬(Phytophthora spp.)、致病疫黴菌(Phytophthora infestans)、寄生疫黴菌(Phytophthora parasitica)、大豆疫黴菌(Phytophthora sojae)、辣椒疫黴菌(Phytophthora capsici)、樟疫黴菌(Phytophthora cinnamon)、草莓疫黴菌(Phytophthora fragariae)、疫黴菌屬、櫟樹猝死病菌(Phytophthora ramorum)、棕櫚疫黴菌(Phytophthora palmivara)、菸草疫黴菌(Phytophthora nicotianae)、層鏽菌屬、豆薯層鏽菌、層鏽層假尾孢菌(Phakopsora meibomiae)、曲黴菌屬(Aspergillus spp.)、黃麴黴(Aspergillus flavus)、黑麯黴(Aspergillus niger)、單孢鏽菌屬(Uromyces spp.)、菜豆銹病菌、枝孢菌屬(Cladosporium spp.)、多主枝孢黴(Cladosporium herbarum)、根黴菌屬(Rhizopus spp.)、少根根黴菌(Rhizopus arrhizus)、青黴菌屬(Penicillium spp.)、絲核菌屬、立枯絲核菌(Rhizoctonia solani)、玉蜀黍絲核菌(Rhizoctonia zeae)、稻枯斑絲核菌(Rhizoctonia oryzae)、褐色絲核菌(Rhizoctonia caritae)、禾穀絲核菌(Rhizoctonia cerealis)、紫紋羽絲核菌(Rhizoctonia crocorum)、 草莓絲核菌(Rhizoctonia fragariae)、枝生絲核菌(Rhizoctonia ramicola)、覆盆子絲核菌(Rhizoctonia rubi)、豆狀絲核菌(Rhizoctonia leguminicola)、菜豆殼球孢菌(Macrophomina phaseolina)、稻瘟菌(Magnaorthe oryzae)、球腔菌屬、禾生球腔菌(Mycosphaerella graminocola)、斐濟球腔菌(Mycosphaerella fijiensis)(黑色香蕉葉斑病(Black sigatoga))、蘋果斑點球腔菌(Mycosphaerella pomi)、檸檬球腔菌(Mycosphaerella citri)、稻瘟菌屬、稻瘟病菌(Magnaporthe grisea)、鏈核盤菌屬(Monilinia spp.)、桃褐腐病菌(Monilinia fruticola)、藍莓僵果病病原菌(Monilinia vacciniicorymbosi)、核果鏈核盤菌(Monilinia laxa)、炭疽菌屬(Colletotrichum spp.)、膠孢炭疽菌(Colletotrichum gloeosporiodes)、辣椒炭疽菌(Colletotrichum acutatum)、石斛炭疽菌(Colletotrichum Candidum)、間座殼屬(Diaporthe spp.)、柑桔間座殼菌(Diaporthe citri)、棒孢菌屬(Corynespora spp.)、多主棒孢菌(Corynespora Cassiicola)、膠鏽菌屬(Gymnosporangium spp.)、植物受檜膠鏽菌(Gymnosporangium juniperi-virginianae)、裂盾菌屬(Schizothyrium spp.)、仁果裂盾菌(Schizothyrium pomi)、黏殼孢菌屬(Gloeodes spp.)、梨果黏殼孢菌(Gloeodes pomigena)、葡萄座腔菌屬(Botryosphaeria spp.)、葡萄潰瘍病病菌(Botryosphaeria dothidea)、明抱盤菌屬(Neofabraea spp.)、威爾遜孢殼菌屬(Wilsonomyces spp.)、嗜果威爾遜孢殼菌(Wilsonomyces carpophilus)、單囊殼菌屬(Sphaerotheca spp.)、斑點單囊殼菌(Sphaerotheca macularis)、薔薇單囊殼菌(Sphaerotheca pannosa)、白粉菌屬(Erysiphe spp.)、殼多孢菌屬(Stagonospora spp.)、穎枯殼針孢(Stagonospora nodorum)、腐黴屬(Pythium spp.)、終極腐黴菌(Pythium ultimum)、瓜果腐黴菌(Pythium aphanidermatum)、畸雌腐黴菌(Pythium irregularum)、簇囊腐黴菌(Pythium ulosum)、水生腐黴菌 (Pythium lutriarium)、森林腐黴菌(Pythium sylvatium)、黑星菌屬(Venturia spp.)、蘋果黑星病菌(Venturia inaequalis)、輪枝孢菌屬(Verticillium spp.)、黑粉菌屬(Ustilago spp.)、裸黑粉菌(Ustilago nuda)、玉蜀黍黑粉菌(Ustilago maydis)、甘蔗鞭黑粉菌(Ustilago scitaminea)、麥角菌屬(Claviceps spp.)、麥角菌(Claviceps puprrea)、腥黑粉菌屬(Tilletia spp.)、小麥腥黑粉菌(Tilletia tritici)、光滑腥黑粉菌(Tilletia laevis)、稻粒黑粉菌(Tilletia horrid)、小麥矮腥黑粉菌(Tilletia controversa)、莖點黴屬(Phoma spp.)、大豆生莖點黴(Phoma glycinicola)、多變莖點黴(Phoma exigua)、甘藍莖點黴(Phoma lingam)、禾旋孢腔菌(Cocliobolus sativus)、禾頂囊殼(Gaeumanomyces gaminis)、炭疽菌屬(Colleototricum spp.)、雲紋病菌屬(Rhychosporium spp.)、大麥雲紋病菌(Rhychosporium secalis)、離蠕孢屬(Biopolaris spp.)、長蠕孢屬(Helminthosporium spp.)、大麥長蠕孢(Helminthosporium secalis)、玉蜀黍長蠕孢(Helminthosporium maydis)、索萊長蠕孢(Helminthosporium solai)及小麥褐斑長蠕孢(Helminthosporium tritici-repentis)或其組合。 In the compositions and methods of the present invention, pathogenic infections can be caused by a variety of plant pathogens including, for example, but not limited to, plant fungal pathogens, plant bacterial pathogens, rust, Botrytis, Botrytis cinerea, Botrytis squamosa , Erwinia, Erwinia carotovora , Erwinia amylovora , Dickeya spp. , Dickeya Daddantii ), Dickeya solani , Agrobacterium spp. , Agrobacterium tumefaciens , Xanthomonas, Xanthomonas axonopodis , Xanthomonas campestris Xanthomonas campestris pv. carotae , Xanthomonas pruni , Xanthomonas arboricola , Xanthomonas oryzae pv. oryzae , Saccharomyces cerevisiae Genus ( Xylella spp. ), Xylella fastidiosa , Candidatus spp., phloem ( Can Didatus liberibacter ), Fusarium genus, Fusarium colmorum , Fusarium graminearum, Fusarium oxysporum , Fusarium oxysporum f.sp. Cubense , Fusarium oxysporum f .sp.Lycopersici ), Fusarium virguliforme , Sclerotinia spp. , Sclerotinia sclerotiorum , Sclerotinia minor , Sclerotinia Homeocarpa ), Cercospora/Cercosporidium spp., Uncinula spp., Uncinula necator (Powdery Mildew), Podosphaera spp () Powdery mildew), Podosphaera leucotricha , Podosphaera clandestine , Phomopsis spp., Phomopsis Viticola ), Alternaria spp. , Alternaria tenuissima , Alternaria porri , Alternaria alternate , Alzheimer's Alternaria tenuis , Pseudomonas, Pseudomonas syringae pv. Tomato , Phytophthora spp., Phytophthora infestans ), Phytophthora parasitica , Phytophthora sojae , Phytophthora capsici , Phytophthora cinnamon , Phytophthora fragariae , Phytophthora, eucalyptus ( Phytophthora ramorum ), Phytophthora palmivara , Phytophthora nicotianae , Puccinia, Phakopsora pylori, Phakopsora meibomiae , Aspergillus spp . ), Aspergillus flavus , Aspergillus niger , Uromyces spp. , Bean rust, Cladosporium spp. , Cladosporium Herbarum ), Rhizopus spp., Rhizopus arrhizus , Penicillium spp., Rhizoctonia Genus, Rhizoctonia solani , Rhizoctonia zeae, Rhizoctonia oryzae , Rhizoctonia caritae , Rhizoctonia cerealis Rhizoctonia crocorum , Rhizoctonia fragariae , Rhizoctonia ramicola , Rhizoctonia rubi , Rhizoctonia leguminicola , Rhizoctonia leguminicola , Rhizoctonia leguminicola Macrophomina phaseolina , Magnaorthe oryzae , Cochlear , Mycosphaerella graminocola , Mycosphaerella fijiensis (Black sigatoga) ), Mycosphaerella pomi , Mycosphaerella citri , Magnaporthe oryzae, Magnaporthe grisea , Monilinia spp., Peach brown rot Monilinia fruticola ), Monilinia vacciniicorymbosi , Monilinia laxa , Anthracnose ( Colletotrichu) m spp.), Colletotrichum gloeosporiodes , Colletotrichum acutatum , Colletotrichum Candidum , Diaporthe spp., Diaporthe citri , the genus Micromonospora rod (Corynespora spp.), multi-master rod cinerea (Corynespora Cassiicola), the genus gum rust (Gymnosporangium spp.), gum juniper plants by rust (Gymnosporangium juniperi-virginianae), Scarshield genus (Schizothyrium Spp. ), Schizothyrium pomi , Gloeodes spp., Gloeodes pomigena , Botryosphaeria spp., grape canker Pathogen ( Botryosphaeria dothidea ), Neofabraea spp., Wilsonomyces spp., Wilsonomyces carpophilus , Sphaerotheca spp. Sphaerotheca macularis , Sphaerotheca pannosa , Erysiphe spp., Stagonospora spp. , and A. sinensis Stagonospora nodorum ), Pythium spp. , Pythium ultimum , Pythium aphanidermatum , Pythium irregularum , Pythium ulosum , aquatic rot Pythium lutriarium , Pythium sylvatium , Venturia spp. , Venturia inaequalis , Verticillium spp., Ustilago Spp. ), Ustilago nuda , Ustilago maydis , Ustilago scitaminea , Claviceps spp. , Claviceps puprrea , Tilletia spp. , Tilletia tritici , Tilletia laevis , Tilletia horrid , Tilletia controversa ), Phoma spp. , Phoma glycinicola , Phoma exigua , Phoma lingam , Cocliobolus sativus , Gaeumannomyces (Gaeumanomyces gaminis), the genus Colletotrichum (Colleototricum spp.), Moire bacteria genus (Rhychosporium spp.), Barley germs moire (Rhychosporium secalis), from Helminthosporium (Biopolaris spp.), Helminthosporium ( Helminthosporium spp. ), Helminthosporium secalis , Helminthosporium maydis , Helminthosporium solai , and Helminthosporium tritici-repentis or combinations thereof.

在一些實施例中，病原性感染可由以下一者或組合引起：大豆鏽菌(豆薯層鏽菌、層鏽層假尾孢菌)且植物包含大豆；灰葡萄孢菌(葡萄孢疫病)且植物包含葡萄；灰葡萄孢菌(葡萄孢疫病)且植物包含草莓；交鏈孢菌屬(例如立枯交鏈孢菌)且植物包含番茄；交鏈孢菌屬(例如立枯交鏈孢菌)且植物包含馬鈴薯；豆銹病(菜豆銹病菌)且植物包含常見豆子；白粉病菌(大豆白粉病)且植物包含大豆；斐濟球腔菌(黑色香蕉葉斑病)或古巴尖孢鐮刀菌(巴拿馬疾病)且植物包含香蕉；黃單孢菌屬或水稻黃單孢菌水稻變種且植物包含稻穀；地毯草黃單孢菌且植物包含木薯；野油菜黃單孢菌且植物包含番茄；灰葡萄孢菌(胡椒葡萄孢疫病)且植物包含胡椒；白粉病且植物包含葫蘆；向日葵核盤菌 (白黴)且植物包含食莢菜豆；向日葵核盤菌(白黴)且植物包含馬鈴薯；同果核盤菌(幣斑病)且植物包含草皮草；南方白黴且植物包含花生；葉斑病(尾孢菌/尾孢菌)且植物包含花生；禾穀鐮刀菌(小麥赤黴病)且植物包含小麥；禾生球腔菌(小麥殼針孢斑)且植物包含小麥；穎枯殼針孢(穎枯病及殼針孢葉枯病)且植物包含小麥；解澱粉歐文菌且植物包含蘋果、梨及其他梨果果實；蘋果黑星病菌且植物包含蘋果、梨及其他梨果果實；或立枯絲核菌且植物包含小麥、稻穀、草皮草、大豆、玉米、豆科植物及蔬菜作物。 In some embodiments, the pathogenic infection can be caused by one or a combination of: soybean rust (Phaeophyllum serrata, Pseudomonas aeruginosa) and the plant comprises soybean; Botrytis cinerea (B. blight) and Plants contain grapes; Botrytis cinerea (B. blight) and plants contain strawberries; Alternaria (such as Alternaria alternata) and plants contain tomatoes; Alternaria (such as Alternaria alternata) And the plant contains potato; bean rust (String rust) and the plant contains common beans; powdery mildew (soybean powdery mildew) and the plant contains soybean; Fischerella fibrin (black banana leaf spot) or Fusarium oxysporum (Panama) Disease) and the plant comprises banana; Xanthomonas or Xanthomonas oryzae rice variety and the plant comprises rice; Rhizoctonia solani and the plant comprises cassava; Xanthomonas campestris and the plant comprises tomato; Botrytis cinerea Bacteria (Pepperum blight) and plants containing pepper; powdery mildew and plants containing gourd; (White mold) and the plant comprises a pod bean; a sunflower sclerotiorum (white mold) and the plant comprises a potato; a sclerotinia (coin spot disease) and the plant comprises a turf grass; a southern white mold and the plant comprises a peanut; a leaf spot Disease (C. cerevisiae / Cercospora) and the plant contains peanuts; Fusarium graminearum (branches of wheat) and the plant contains wheat; the genus Coriolus sinensis (the wheat bursa) and the plant contains wheat; Spores (blight blight and Phytophthora capsici) and plants comprising wheat; starch-destroying worms and plants comprising apples, pears and other pear fruit; apple scab and plants comprising apples, pears and other pear fruits; Or Rhizoctonia solani and plants containing wheat, rice, turf, soybean, corn, legumes and vegetable crops.

包括RTI472菌株之組合物可呈液體、油性分散液、粉劑、乾燥可潤濕粉末、可撒佈顆粒或乾燥可潤濕顆粒之形式。當組合物施用於植物葉、植物莖皮、植物果實、植物花、植物種子、植物根部、植物剪枝、植物移植物、植物癒合組織；植物周圍土壤或生長介質；在土壤或生長介質中播種植物種子之前的土壤或生長介質；或在土壤或生長介質中種植植物、植物剪枝、植物移植物或植物癒合組織之前的土壤或生長介質時，當以適合於使植物生長獲益及/或對易感病植物賦予保護以避免病原性感染之量施用時，該組合物使植物生長獲益。組合物可進一步包括微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑、除草劑、植物提取物、植物生長調節劑或肥料之一或組合，其以適合於使植物生長獲益及/或對易感病植物賦予保護以避免病原性感染之量存在。組合物可進一步包括載劑、界面活性劑、分散劑或酵母提取物之一或組合。出於本說明書及申請專利範圍之目的，術語「界面活性劑」及「佐劑」可互換使用。酵母提取物可以在約0.01%至0.2%w/w範圍內的使植物生長獲益之施用率輸送。 Compositions comprising the RTI 472 strain may be in the form of a liquid, an oily dispersion, a powder, a dry wettable powder, a spreadable granule or a dry wettable granule. When the composition is applied to plant leaves, plant bark, plant fruits, plant flowers, plant seeds, plant roots, plant pruning, plant transplants, plant healing tissues; soil or growth medium surrounding the plant; seeding in soil or growth medium Soil or growth medium prior to plant seeds; or soil or growth medium prior to planting plants, plant pruning, plant transplants, or plant healing tissues in soil or growth media, when suitable for plant growth benefits and/or The composition benefits plant growth when administered to a susceptible plant to protect against the pathogenic infection. The composition may further comprise one or a combination of a microbial, biological or chemical insecticide, fungicide, nematicide, bactericide, herbicide, plant extract, plant growth regulator or fertilizer, suitable for growing the plant Benefits and/or protection of susceptible plants to avoid the presence of pathogenic infections. The composition may further comprise one or a combination of a carrier, a surfactant, a dispersant or a yeast extract. For the purposes of this specification and the scope of the patent application, the terms "interacting agent" and "adjuvant" are used interchangeably. The yeast extract can be delivered at an application rate that benefits plant growth in the range of about 0.01% to 0.2% w/w.

組合物可呈種植基質形式。種植基質可呈盆栽土形式。 The composition can be in the form of a planting matrix. The planting substrate can be in the form of potting soil.

在一個實施例中，提供一種組合物，其包含：一或多種具有使植物生長或植物健康中之一或兩者獲益之特性的微生物之生物純培養 物；以及酵母提取物，該組合物用於施用於植物以達成使植物生長獲益或對易感病植物賦予保護以避免病原性感染中的一或兩者。微生物可包含真菌物種。微生物可包含細菌物種。微生物可包含芽孢桿菌屬微生物。微生物可包含解澱粉芽孢桿菌。組合物可進一步包含載劑、界面活性劑或分散劑之一或組合。組合物可進一步包含微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑、除草劑、植物提取物、植物生長調節劑或肥料之一或組合。 In one embodiment, a composition is provided comprising: one or more biologically pure cultures of microorganisms having properties that benefit one or both of plant growth or plant health And yeast extracts for application to plants to achieve one or both of the benefits of plant growth or protection of susceptible plants to avoid pathogenic infections. Microorganisms can comprise fungal species. Microorganisms can comprise bacterial species. The microorganism may comprise a microorganism of the genus Bacillus. The microorganism may comprise Bacillus amyloliquefaciens. The composition may further comprise one or a combination of a carrier, a surfactant or a dispersing agent. The composition may further comprise one or a combination of a microbial, biological or chemical insecticide, fungicide, nematicide, bactericide, herbicide, plant extract, plant growth regulator or fertilizer.

在一個實施例中，提供用於達成使植物生長獲益或對易感病植物賦予保護以避免病原性感染中之一或兩者的組合物，該組合物包括：以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物，呈適合於使植物生長獲益及/或對易感病植物賦予保護以避免病原性感染之量；以及微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑、除草劑、植物提取物、植物生長調節劑或肥料之一或組合，呈適合於使植物生長獲益及/或對易感病植物賦予保護以避免病原性感染之量。在此實施例中，解澱粉芽孢桿菌RTI472之生物純培養物及微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑、除草劑、植物提取物、植物生長調節劑或肥料之一或組合調配在一起。 In one embodiment, a composition for achieving one or both of the benefits of plant growth or protection of a susceptible plant to avoid pathogenic infection is provided, the composition comprising: ATCC No. PTA-121166 A biologically pure culture of the deposited Bacillus amyloliquefaciens RTI472 or a mutant thereof having all of its identifying characteristics, in an amount suitable to benefit plant growth and/or to confer protection to susceptible plants to avoid pathogenic infection; One or a combination of a microbial, biological or chemical insecticide, fungicide, nematicide, bactericide, herbicide, plant extract, plant growth regulator or fertilizer, suitable for benefiting plant growth and/or Susceptible plants confer protection to avoid pathogenic infections. In this embodiment, a biologically pure culture of Bacillus amyloliquefaciens RTI472 and a microbial, biological or chemical insecticide, fungicide, nematicide, bactericide, herbicide, plant extract, plant growth regulator or fertilizer One or a combination is blended together.

在一個實施例中，殺真菌劑可包括來自白羽扇豆之提取物。在一個實施例中，殺真菌劑可包括BLAD多肽。BLAD多肽可為來自甜羽扇豆(白羽扇豆)之天然存在之種子儲存蛋白的片段，其藉由對真菌細胞壁造成損害且破壞內細胞膜而作用於易感真菌病原體。組合物可包括約20%BLAD多肽。 In one embodiment, the fungicide may comprise an extract from white lupin. In one embodiment, the fungicide may comprise a BLAD polypeptide. The BLAD polypeptide may be a fragment of a naturally occurring seed storage protein from sweet lupin (white lupin) that acts on a susceptible fungal pathogen by causing damage to the fungal cell wall and disrupting the inner cell membrane. The composition can include about 20% BLAD polypeptide.

在包括解澱粉芽孢桿菌RTI472之組合物中，組合物可呈液體形式且解澱粉芽孢桿菌RTI472可以約1.0×108CFU/ml至約1.0×1012CFU/ml之濃度存在。組合物可呈粉劑、乾燥可潤濕粉末、可撒佈顆 粒或乾燥可潤濕顆粒之形式且解澱粉芽孢桿菌RTI472可以約1.0×108CFU/g至約1.0×1012CFU/g之量存在。組合物可呈油性分散液形式，且解澱粉芽孢桿菌RTI472可以約1.0×109CFU/ml至約1.0×1012CFU/ml之濃度存在。解澱粉芽孢桿菌RTI472可呈孢子或營養細胞形式。 In compositions comprising B. amyloliquefacidis RTI 472, the composition may be in liquid form and B. amyloliquefaciens RTI 472 may be present at a concentration of from about 1.0 x 10 8 CFU/ml to about 1.0 x 10 12 CFU/ml. The composition may be in the form of a powder, a dry wettable powder, a spreadable granule or a dry wettable granule and the B. amyloliquefaciens RTI 472 may be in an amount from about 1.0 x 10 8 CFU/g to about 1.0 x 10 12 CFU/g. presence. The composition may be in the form of an oily dispersion, and the B. amyloliquefaciens RTI 472 may be present at a concentration of from about 1.0 x 10 9 CFU/ml to about 1.0 x 10 12 CFU/ml. Bacillus amyloliquefaciens RTI472 can be in the form of spores or vegetative cells.

在一個實施例中，提供一種用於達成使植物生長獲益或對易感病植物賦予保護以避免病原性感染中之一或兩者的方法，該方法包括：將包含以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物的組合物輸送至：植物葉、植物莖皮、植物果實、植物花、植物種子、植物根部、植物剪枝、植物移植物、植物癒合組織；植物周圍土壤或生長介質；在土壤或生長介質中播種植物種子之前的土壤或生長介質；或在土壤或生長介質中種植植物、植物剪枝、植物移植物或植物癒合組織之前的土壤或生長介質，該組合物之呈適合於使植物生長獲益及/或對易感病植物賦予保護以避免病原性感染之量。組合物可輸送至植物葉。 In one embodiment, a method for achieving one or both of plant growth benefit or protection of a susceptible plant to avoid pathogenic infection is provided, the method comprising: comprising ATCC PTA-121166 A composition of the deposited biologically pure culture of Bacillus amyloliquefaciens RTI472 or a mutant thereof having all of its identifying characteristics is delivered to: plant leaves, plant bark, plant fruits, plant flowers, plant seeds, plant roots, plant shears Branches, plant grafts, plant healing tissues; soils or growth media surrounding plants; soils or growth media prior to planting plant seeds in soil or growth media; or planting plants, plant pruning, plant grafts in soil or growth media Or the soil or growth medium prior to the healing of the tissue, the composition being present in an amount suitable to benefit plant growth and/or to confer protection to susceptible plants to avoid pathogenic infections. The composition can be delivered to plant leaves.

在該方法中，包括解澱粉芽孢桿菌RTI472之組合物可進一步包括載劑、界面活性劑、分散劑或酵母提取物之一或組合。酵母提取物可以在約0.01%至0.2%w/w範圍內的使植物生長獲益之施用率輸送。 In this method, the composition comprising Bacillus amyloliquefaciens RTI 472 may further comprise one or a combination of a carrier, a surfactant, a dispersing agent or a yeast extract. The yeast extract can be delivered at an application rate that benefits plant growth in the range of about 0.01% to 0.2% w/w.

在本發明之另一實施例中，提供一種用於達成使植物生長獲益或對易感病植物賦予保護以避免病原性感染中之一或兩者的方法，該方法包括將一種組合物輸送至植物葉、植物莖皮、植物果實、植物花、植物種子、植物根部、植物剪枝、植物移植物、植物癒合組織；植物周圍土壤或生長介質；在土壤或生長介質中播種植物種子之前的土壤或生長介質；或在土壤或生長介質中種植植物、植物剪枝、植物移植物或植物癒合組織之前的土壤或生長介質，該組合物包含以下各物：以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有 其所有鑑別特徵之突變體的生物純培養物，呈適合於使植物生長獲益及/或對易感病植物賦予保護以避免病原性感染之量；以及微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑、除草劑、植物提取物、植物生長調節劑或肥料之一或組合，呈適合於使植物生長獲益及/或賦予保護以避免病原性感染之量。 In another embodiment of the present invention, there is provided a method for achieving one or both of benefiting plant growth or conferring protection to a susceptible plant to avoid a pathogenic infection, the method comprising delivering a composition To plant leaves, plant stems, plant fruits, plant flowers, plant seeds, plant roots, plant pruning, plant transplants, plant healing tissues; soil or growth medium around plants; before plant seeds are planted in soil or growth medium Soil or growth medium; or soil or growth medium prior to planting plants, plant pruning, plant transplants or plant healing tissues in soil or growth medium, the composition comprising the following: deposited under ATCC PTA-121166 Bacillus amyloliquefaciens RTI472 or A biologically pure culture of mutants of all of the identified features, in an amount suitable to benefit plant growth and/or to confer protection to susceptible plants to avoid pathogenic infections; and microbial, biological or chemical insecticides, killing One or a combination of fungicides, nematicides, bactericides, herbicides, plant extracts, plant growth regulators or fertilizers is in an amount suitable to benefit plant growth and/or to confer protection against pathogenic infections.

在一個實施例中，提供一種用於達成使植物生長獲益或對易感病植物賦予保護以避免病原性感染中之一或兩者的方法，該方法包括：將第一組合物與第二組合物之組合輸送至植物葉、植物莖皮、植物果實、植物花、植物種子、植物根部、植物剪枝、植物移植物、植物癒合組織；植物周圍土壤或生長介質；在土壤或生長介質中播種植物種子之前的土壤或生長介質；或在土壤或生長介質中種植植物、植物剪枝、植物移植物或植物癒合組織之前的土壤或生長介質，該第一組合物包含以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物，呈適合於使植物生長獲益及/或對易感病植物賦予保護以避免病原性感染之量；且該第二組合物包含微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑、除草劑、植物提取物、植物生長調節劑或肥料之一或組合，呈適合於使植物生長獲益及/或對易感病植物賦予保護以避免病原性感染之量。在一個實施例中，第一及第二組合物可輸送至植物葉。第一組合物可進一步包括載劑、界面活性劑、分散劑或酵母提取物之一或組合。酵母提取物可以在約0.01%至0.2%w/w範圍內的使植物生長獲益之施用率輸送。 In one embodiment, a method for achieving one or both of benefiting plant growth or conferring protection to a susceptible plant to avoid a pathogenic infection is provided, the method comprising: treating the first composition with the second The combination of the composition is delivered to plant leaves, plant bark, plant fruits, plant flowers, plant seeds, plant roots, plant pruning, plant transplants, plant healing tissues; soil or growth medium surrounding the plant; in soil or growth medium The soil or growth medium prior to seeding the plant seed; or the soil or growth medium prior to planting the plant, plant pruning, plant graft or plant healing tissue in the soil or growth medium, the first composition comprising ATCC PTA-121166 A biologically pure culture of the deposited B. amyloliquefaciens RTI472 or a mutant thereof having all of its identifying characteristics, in an amount suitable to benefit plant growth and/or to confer protection to susceptible plants to avoid pathogenic infections; And the second composition comprises a microbial, biological or chemical insecticide, a fungicide, a nematicide, a bactericide, a herbicide, a plant extract Plant growth regulators or fertilizers or one combination, adapted to the form and / or amount of the benefit plant growth disease susceptible plants to confer protection to prevent pathogenic infection. In one embodiment, the first and second compositions can be delivered to the foliage of the plant. The first composition may further comprise one or a combination of a carrier, a surfactant, a dispersant, or a yeast extract. The yeast extract can be delivered at an application rate that benefits plant growth in the range of about 0.01% to 0.2% w/w.

第二組合物之殺真菌劑可包括來自白羽扇豆之提取物。第二組合物之殺真菌劑可包括BLAD多肽。BLAD多肽可為來自甜羽扇豆(白羽扇豆)之天然存在之種子儲存蛋白的片段，其藉由對真菌細胞壁造成損害且破壞內細胞膜而作用於易感真菌病原體。第二組合物之殺真 菌劑可包括約20%BLAD多肽。 The fungicide of the second composition may comprise an extract from white lupin. The fungicide of the second composition can include a BLAD polypeptide. The BLAD polypeptide may be a fragment of a naturally occurring seed storage protein from sweet lupin (white lupin) that acts on a susceptible fungal pathogen by causing damage to the fungal cell wall and disrupting the inner cell membrane. Killing the second composition The microbial agent can include about 20% BLAD polypeptide.

在用於輸送RTI472與微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑、除草劑、植物提取物、植物生長調節劑或肥料組合的本發明之組合物及方法中，植物生長益處及/或所賦予之保護可藉由幼苗活力提高、根發育改善、植物生長改善、植物健康提高、產量增加、外觀改善、對植物病原體之抗性提高、病原性感染減少或其組合展現。 In the compositions and methods of the present invention for delivering RTI 472 in combination with a microbial, biological or chemical insecticide, fungicide, nematicide, bactericide, herbicide, plant extract, plant growth regulator or fertilizer, Plant growth benefits and/or protection afforded by increased seedling vigor, improved root development, improved plant growth, improved plant health, increased yield, improved appearance, increased resistance to plant pathogens, reduced pathogenic infection, or combinations thereof Show.

在一個實施例中，該方法可進一步包括向以下各者施用液體肥料：植物周圍土壤或生長介質；在土壤或生長介質中播種植物種子之前的土壤或生長介質；或在土壤或生長介質中種植植物、植物剪枝、植物移植物或植物癒合組織之前的土壤或生長介質。 In one embodiment, the method may further comprise applying a liquid fertilizer to: soil or growth medium surrounding the plant; soil or growth medium prior to seeding the plant seed in the soil or growth medium; or planting in soil or growth medium Soil or growth medium prior to plant, plant pruning, plant graft or plant healing tissue.

在用於輸送RTI472與微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑、除草劑、植物提取物、植物生長調節劑或肥料組合之方法中，組合物可呈液體、油性分散液、粉劑、乾燥可潤濕粉末、可撒佈顆粒或乾燥可潤濕顆粒形式。解澱粉芽孢桿菌RTI472可呈孢子或營養細胞形式。解澱粉芽孢桿菌RTI472可以約1.0×1010CFU/ha至約1.0×1014CFU/ha之使植物生長獲益之施用率輸送。酵母提取物可以在約0.01%至0.2%w/w範圍內的使植物生長獲益之施用率輸送。 In a method for delivering RTI 472 in combination with a microbial, biological or chemical insecticide, fungicide, nematicide, bactericide, herbicide, plant extract, plant growth regulator or fertilizer, the composition may be in the form of a liquid, Oily dispersions, powders, dry wettable powders, spreadable granules or dry wettable granules. Bacillus amyloliquefaciens RTI472 can be in the form of spores or vegetative cells. Bacillus amyloliquefaciens RTI 472 can be delivered at an application rate of about 1.0 x 10 10 CFU/ha to about 1.0 x 10 14 CFU/ha for plant growth benefit. The yeast extract can be delivered at an application rate that benefits plant growth in the range of about 0.01% to 0.2% w/w.

在輸送RTI472與微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑、除草劑、植物提取物、植物生長調節劑或肥料之本發明之組合物及方法中，殺蟲劑可包含畢芬寧。在一或多個實施例中，殺線蟲劑可包含硫線磷(cadusafos)。在一或多個實施例中，殺蟲劑可包含畢芬寧且組合物可調配為液體。在一或多個實施例中，殺蟲劑可包含畢芬寧及可尼丁。在一或多個實施例中，殺蟲劑可包含畢芬寧及可尼丁且組合物可調配為液體。在一或多個實施例中，殺蟲劑可包含畢芬寧或ξ-賽滅寧。在一或多個實施例中，組合物可調配為液體且殺 蟲劑可包含畢芬寧或ξ-賽滅寧。 In the composition and method of the present invention for transporting RTI 472 and microbial, biological or chemical insecticides, fungicides, nematicides, bactericides, herbicides, plant extracts, plant growth regulators or fertilizers, insecticides It can contain Bifenin. In one or more embodiments, the nematicide can comprise cadusafos. In one or more embodiments, the pesticide can comprise diphennin and the composition can be formulated as a liquid. In one or more embodiments, the pesticide can comprise diphennin and cotinine. In one or more embodiments, the pesticide can comprise diphennin and cotinine and the composition can be formulated as a liquid. In one or more embodiments, the pesticide may comprise diphennin or acetonin. In one or more embodiments, the composition can be formulated as a liquid and killed The insecticide may comprise bifenin or acetonin.

殺蟲劑可為畢芬寧，且組合物調配物可進一步包含水合鋁-鎂矽酸鹽及選自由以下組成之群的至少一種分散劑：蔗糖酯、木質素磺酸鹽、烷基多醣苷、萘磺酸甲醛縮合物及磷酸酯。畢芬寧殺蟲劑可以範圍為0.1g/ml至0.2g/ml之濃度存在。畢芬寧殺蟲劑可以約0.1715g/ml之濃度存在。畢芬寧殺蟲劑之施用率可在每公頃約0.1公克畢芬寧(g ai/ha)至約1000g ai/ha範圍內，更佳在約1g ai/ha至約100g ai/ha範圍內。 The insecticide may be bifenin, and the composition formulation may further comprise hydrated aluminum-magnesium citrate and at least one dispersant selected from the group consisting of sucrose esters, lignosulfonates, alkyl polyglycosides, naphthalenes. Sulfonic acid formaldehyde condensate and phosphate ester. Bifenin insecticides can be present in concentrations ranging from 0.1 g/ml to 0.2 g/ml. The bifenin insecticide can be present at a concentration of about 0.1715 g/ml. The application rate of the Bifenin insecticide may range from about 0.1 gram per gram of bifenine (g ai/ha) to about 1000 g ai/ha, more preferably from about 1 g ai/ha to about 100 g ai/ha.

在一實施例中，畢芬寧組合物可包含：畢芬寧；水合鋁-鎂矽酸鹽；以及至少一種選自以下之分散劑：蔗糖酯、木質素磺酸鹽、烷基多醣苷、萘磺酸甲醛縮合物及磷酸酯。 In one embodiment, the Phenfenin composition may comprise: phenfenamicin; hydrated aluminum-magnesium citrate; and at least one dispersant selected from the group consisting of sucrose esters, lignosulfonates, alkyl polyglycosides, naphthalenesulfonic acid formaldehyde Condensate and phosphate.

畢芬寧可較佳以按組合物中所有組分之總重量計1.0重量%至35重量%，更尤其15重量%至25重量%之濃度存在。畢芬寧殺蟲劑組合物可以適合於與肥料混合為液體之方式調配。畢芬寧殺蟲劑組合物可以在0.1g/ml至0.2g/ml範圍內之濃度存在於液體調配物。畢芬寧殺蟲劑可以約0.1715g/ml之濃度存在於液體調配物。術語「可以適合於與肥料混合為液體之方式調配」及「呈與液體肥料相容之調配物」在本文中整個說明書中及申請專利範圍中可互換使用且意欲意謂調配物能夠溶解或分散或乳化於水溶液中以允許與肥料混合用於在液體調配物中輸送至植物。 Bifenin may preferably be present at a concentration of from 1.0% to 35% by weight, more particularly from 15% to 25% by weight, based on the total weight of all components in the composition. The Bifenin insecticide composition can be formulated to be mixed with a fertilizer to form a liquid. The Phenfenin insecticidal composition can be present in the liquid formulation at a concentration ranging from 0.1 g/ml to 0.2 g/ml. The bifenin insecticide can be present in the liquid formulation at a concentration of about 0.1715 g/ml. The terms "may be suitable for mixing with fertilizers as a liquid" and "formulations compatible with liquid fertilizers" are used interchangeably throughout the specification and patent application and are intended to mean that the formulation is capable of dissolving or dispersing. Emulsified in an aqueous solution to allow mixing with the fertilizer for delivery to the plant in a liquid formulation.

分散劑可較佳以按組合物中所有組分之總重量計約0.02重量%至約20重量%之總濃度存在。 The dispersing agent may preferably be present in a total concentration of from about 0.02% by weight to about 20% by weight based on the total weight of all components of the composition.

在一些實施例中，水合鋁-鎂矽酸鹽可選自由蒙脫石及綠坡縷石組成之群。 In some embodiments, the hydrated aluminum-magnesium silicate may be selected from the group consisting of montmorillonite and attapulgite.

在一些實施例中，磷酸酯可選自壬基酚磷酸酯及十三烷醇乙氧基化磷酸酯鉀鹽。 In some embodiments, the phosphate ester can be selected from the group consisting of nonylphenol phosphate and tridecyl alcohol ethoxylated phosphate potassium salt.

其他實施例可進一步包括抗凍劑、消泡劑及殺生物劑中之至少一者。 Other embodiments may further include at least one of an antifreeze, an antifoaming agent, and a biocide.

在一個實施例中，提供一種用於使植物生長獲益之組合物，該組合物包含：以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有所有其鑑別特徵之突變體的生物純培養物；以及殺蟲劑。殺蟲劑可為擬除蟲菊酯(pyrethroid)、畢芬寧、七氟菊酯(tefluthrin)、ξ-賽滅寧、有機磷酸酯、氯氧磷(chlorethoxyfos)、陶斯松(chlorpyrifos)、丁基嘧啶磷(tebupirimfos)、賽扶寧(cyfluthrin)、非潑羅(fiprole)、費普尼(fipronil)、菸鹼類(nicotinoid)或可尼丁(clothianidin)之一或組合。殺蟲劑可包括畢芬寧。殺蟲劑可包括畢芬寧且組合物可呈與液體肥料相容之調配物。組合物可進一步包括水合鋁-鎂矽酸鹽及至少一種分散劑。畢芬寧殺蟲劑可以在0.1g/ml至0.2g/ml範圍內之濃度存在。畢芬寧殺蟲劑可以約0.1715g/ml之濃度存在。 In one embodiment, a composition for benefiting plant growth is provided, the composition comprising: Bacillus amyloliquefaciens RTI472 deposited with ATCC PTA-121166 or a mutant thereof having all of its identifying characteristics Pure culture; and insecticides. Insecticides may be pyrethroid, bifennin, tefluthrin, guanidine, organophosphate, chlorethoxyfos, chlorpyrifos, butyl pyrimidine One or a combination of (tebupirimfos), cyfluthrin, fiprole, fipronil, nicotinoid or clothianidin. The insecticide can include diphennin. The insecticide can include diphennin and the composition can be formulated to be compatible with liquid fertilizers. The composition may further comprise hydrated aluminum-magnesium silicate and at least one dispersant. The bifenin insecticide can be present in a concentration ranging from 0.1 g/ml to 0.2 g/ml. The bifenin insecticide can be present at a concentration of about 0.1715 g/ml.

另外，在一或多個實施例中，本發明之組合物及方法之適合殺蟲劑、除草劑、殺真菌劑及殺線蟲劑可包括以下： Additionally, in one or more embodiments, suitable insecticides, herbicides, fungicides, and nematicides of the compositions and methods of the present invention can include the following:

殺蟲劑：A0)各種殺蟲劑，包括農用殺蟲劑(agrigata)、磷化鋁、鈍綏蟎(amblyseius)、蚜小蜂(aphelinus)、蚜繭蜂(aphidius)、食蚜蜂(aphidoletes)、青蒿素(artimisinin)、苜蓿銀紋夜蛾NPV(autographa californica NPV)、***錫(azocyclotin)、枯草芽孢桿菌、蘇雲金芽孢桿菌屬亞莎華亞種(Bacillus thuringiensis-spp.aizawai)、蘇雲金芽孢桿菌屬庫斯塔克亞種(Bacillus thuringiensis spp.kurstaki)、蘇雲金芽孢桿菌、白僵菌(Beauveria)、球孢白僵菌(Beauveria bassiana)、高效氟氯氰菊酯(betacyfluthrin)、生物製劑、殺蟲雙(bisultap)、溴氟菊酯(brofluthrinate)、溴硫磷-e(bromophos-e)、溴蟎酯(bromopropylate)、Bt-玉米-GM、Bt-大豆-GM、辣椒鹼(capsaicin)、培丹(cartap)、南蛇 藤提取物、氯蟲苯甲醯胺(chlorantraniliprole)、滅幼脲(chlorbenzuron)、氯氧磷(chlorethoxyfos)、克福隆(chlorfluazuron)、陶斯松-e、蛇床子素、冰晶石、殺螟腈(cyanophos)、氰強尼普羅(cyantraniliprole)、賽洛寧(cyhalothrin)、錫蟎丹(cyhexatin)、賽滅寧、離額繭蜂、DCIP、二氯丙烯、三氯殺蟎醇(dicofol)、潛蠅、潛蠅+離額繭蜂、混滅威(dimethacarb)、二硫醚、乙酸十二烷基酯、因滅汀(emamectin)、麗蚜小蜂、EPN、漿角蚜小蜂、二溴化乙烯、桉油醇、脂肪酸、脂肪酸/鹽、芬殺蟎、仲丁威(BPMC)、芬普蟎、溴氟菊酯、氟蟎嗪、複滅蟎、安果、呋線威、γ-賽洛寧、大蒜汁、顆粒體病毒、異色瓢蟲、棉鈴蟲NPV、不活化細菌、吲哚-3-基丁酸、碘甲烷、鐵、水胺硫磷、異丙胺磷、異丙胺磷-m、異丙威、異拌磷、高嶺土、靈丹、瀏陽黴素、苦參鹼、地安磷、聚乙醛、綠僵菌、達馬松、速滅威(MTMC)、礦物油、滅蟻靈、間異硫氰酸鹽、殺蟲單、疣孢漆斑菌、二溴磷、芙新姬小蜂、菸鹼、菸鹼殺蟲劑、油、油酸、氧樂果、小花蝽、氧化苦參鹼、擬青黴菌、石蠟油、巴拉松-e(parathion-e)、巴氏桿菌、石油、信息素、磷酸、光桿狀菌、巴賽松(phoxim)、小植綏蟎、亞特松-e(pirimiphos-e)、植物油、小菜蛾GV、多角體病毒、多酚提取物、油酸鉀、布飛松(profenofos)、補骨內酯、普硫松(prothiofos)、吡唑硫磷、除蟲菊酯、噠嗪硫磷、嘧蟎醚、吡丙醚、皂樹提取物、滅蟎猛、油菜油、魚藤酮、皂素、皂劑(saponozit)、鈉化合物、氟矽酸鈉、澱粉、斯氏線蟲、鏈黴菌、氟蟲胺、硫、丁基嘧啶磷、七氟菊酯、雙硫磷、四氯殺蟎碸、久效威、甲基乙拌磷、轉基因(例如Cry3Bb1)、唑蚜威、木黴菌、赤眼蜂、三福隆、輪枝菌、藜蘆鹼、異構殺蟲劑(例如κ-畢芬寧、κ-七氟菊酯)、介離子殺蟲劑、雙醯胺類殺蟲劑、吡嗪氟醯胺(pyraziflumid)；A1)胺基甲酸酯類，包括得滅克(aldicarb)、棉靈威(alanycarb)、免扶克(benfuracarb)、加保利 (carbaryl)、加保扶(carbofuran)、丁基加保扶(carbosulfan)、滅賜克(methiocarb)、納乃得(methomyl)、歐殺滅(oxamyl)、比加普(pirimicarb)、安丹(propoxur)及硫敵克(thiodicarb)；A2)有機磷酸酯類，包括歐殺松(acephate)、穀速松-乙基(azinphos-ethyl)、穀速松-甲基(azinphos-methyl)、克芬松(chlorfenvinphos)、陶斯松(chlorpyrifos)、陶斯松-甲基(chlorpyrifos-methyl)、內吸磷-S-甲基(demeton-S-methyl)、大利松(diazinon)、二氯松(dichlorvos)/DDVP、雙特松(dicrotophos)、大滅松(dimethoate)、二硫松(disulfoton)、愛殺松(ethion)、撲滅松(fenitrothion)、芬殺松(fenthion)、加福松(isoxathion)、馬拉松(malathion)、達馬松(methamidaphos)、滅大松(methidathion)、美文松(mevinphos)、亞素靈(monocrotophos)、氧樂果(oxymethoate)、甲基滅多松(oxydemeton-methyl)、巴拉松(parathion)、甲基巴拉松(parathion-methyl)、賽達松(phenthoate)、福瑞松(phorate)、裕必松(phosalone)、益滅松(phosmet)、福賜米松(phosphamidon)、甲基亞特松(pirimiphos-methyl)、拜裕松(quinalphos)、託福松(terbufos)、樂本松(tetrachlorvinphos)、三落松(triazophos)及三氯松(trichlorfon)；A3)環戊二烯有機氯化合物類，諸如安殺番(endosulfan)；A4)非潑羅類，包括乙蟲清(ethiprole)、費普尼、氟蟲腈(pyrafluprole)及派瑞樂(pyriprole)；A5)新菸鹼類似物類，包括啶蟲脒(acetamiprid)、可尼丁、呋蟲胺(dinotefuran)、吡蟲啉(imidacloprid)、烯啶蟲胺(nitenpyram)、噻蟲啉(thiacloprid)及噻蟲嗪(thiamethoxam)；A6)賜諾殺類，諸如賜諾殺及斯平托蘭(spinetoram)；A7)來自汀類之氯離子通道活化劑，包括阿巴汀(abamectin)、苯甲酸因滅汀、伊維汀、林皮汀及密滅汀；A8)保幼激素模擬物，諸如烯蟲乙酯、烯蟲炔酯、美賜平(methoprene)、芬諾克(fenoxycarb)及百利普芬(pyriproxyfen)；A9)選擇性同翅目餵養阻斷 劑，諸如派滅淨(pymetrozine)、氟啶蟲醯胺(flonicamid)及哌氟喹腙(pyrifluquinazon)；A10)蟎生長抑制劑，諸如克芬蟎、合賽多及依殺蟎；A11)粒線體ATP合成酶抑制劑，諸如汰芬諾克、芬布錫及毆蟎多；氧化磷酸化去偶合劑，諸如克凡派(chlorfenapyr)；A12)菸鹼乙醯膽鹼受體通道阻斷劑，諸如殺蟲磺(bensultap)、鹽酸培丹、硫賜安(thiocyclam)及殺蟲雙鈉；A13)來自苯甲醯脲類之甲殼素生物合成類型0抑制劑，包括雙三氟蟲脲、二福隆、氟芬隆、六伏隆、祿芬隆、諾伐隆及得福隆；A14)甲殼素生物合成類型1抑制劑，諸如布芬淨；A15)蛻皮瓦解劑，諸如賽滅淨；A16)蛻皮素受體促效劑，諸如滅芬諾、得芬諾、合芬隆及環蟲醯肼；A17)章魚胺受體促效劑，諸如三亞蟎；A18)粒線體複合物電子輸送抑制劑比達本(pyridaben)、得芬瑞(tebufenpyrad)、脫芬瑞(tolfenpyrad)、氟芬內林、腈吡蟎酯、丁氟蟎酯、愛美松、亞醌蟎或嘧蟎酯；A19)電壓依賴性鈉通道阻斷劑，諸如因得克(indoxacarb)及氰氟蟲胺；A20)脂質合成抑制劑，諸如賜派芬、螺甲蟎酯及螺蟲乙酯；A21)來自二醯胺類之利阿諾定受體調節劑，包括氟蟲雙醯胺、鄰苯二甲醯胺化合物(R)-3-氯-N1-{2-甲基-4-[1,2,2,2-四氟-1-(三氟甲基)乙基]苯基}-N2-(1-甲基-2-甲基磺醯基乙基)鄰苯二甲醯胺及(S)-3-氯-N1-{2-甲基-4-[1,2,2,2-四氟-1-(三氟甲基)乙基]苯基}-N2-(1-甲基-2-甲基磺醯基乙基)鄰苯二甲醯胺、氯蟲醯胺(chloranthraniliprole)及氰強尼普羅；A22)未知或不確定作用模式之化合物，諸如印楝素、安米氟美(amidoflumet)、畢芬載(bifenazate)、氟噻蟲碸、協力精、啶蟲丙醚、氟啶蟲胺腈；或A23)來自擬除蟲菊酯類之鈉通道調節劑，包括阿納寧、丙烯除蟲菊酯、畢芬寧、賽扶寧、λ-賽洛寧、賽滅寧、α-賽滅寧、β-賽滅寧、ξ-賽滅寧、第滅寧、益化利、依芬寧、芬普寧、芬化利、護賽寧、τ-福化利、百滅寧、矽護芬及泰滅寧。 Insecticides: A0) various insecticides, including agricultural insecticides (agrigata), aluminum phosphide, amblyseius, aphelinus, aphidius, aphidoletes ), artimisinin, autographa californica NPV, azocyclotin, Bacillus subtilis, Bacillus thuringiensis-spp. aizawai ,库斯塔克亚Bacillus thuringiensis species (Bacillus thuringiensis spp.kurstaki), Bacillus thuringiensis, Beauveria (BEAUVERIA), Beauveria bassiana (Beauveria bassiana), beta-cyfluthrin (betacyfluthrin), biologies, kill Bisultap, brofluthrinate, bromophos-e, bromopropylate, Bt-corn-GM, Bt-soybean-GM, capsaicin, Cartap, South snake vine extract, chlorantraniliprole, chlorbenzuron, chlorethoxyfos, chlorfluazuron, taosson-e, osthole , cryolite, cyanophos, cyanide Cyantraniliprole, cyhalothrin, cyhexatin, cytosine, scorpion venom, DCIP, dichloropropene, dicofol, sinensis, scum, scorpion Dimethacarb, disulfide, dodecyl acetate, emamectin, bee, bee, EPN, bee, dibromide, eucalyptol, fatty acid, fatty acid /Salt, Fenicide, Zhongdingwei (BPMC), fenfluramine, bromide, fluoxazine, retanning, anguilla, furosemide, gamma-cylonine, garlic juice, granulosis virus , heterochromatic ladybug, cotton bollworm NPV, inactivated bacteria, ind-3-butyric acid, methyl iodide, iron, hirudin, isopropylamine, isopropylphosphonium-m, isoprocarb, isophosphorus, Kaolin, Lingdan, Liuyangmycin, matrine, diammine, polyacetaldehyde, Metarhizium, Damasson, quetiapine (MTMC), mineral oil, mirex, isothiocyanate, Insecticidal, Fusarium oxysporum, dibromophosphorus, Fuxinji wasp, nicotine, nicotine insecticide, oil, oleic acid, omethoate, small flower bud, oxymatrine, pseudomycin, Paraffin oil, Lathon-e (parathion-e), pasteurella, petroleum, pheromone, phosphoric acid, light bacillus, phoxim, vegetative, pirimiphos-e, vegetable oil, Plutella xylostella GV, polyhedrosis virus, polyphenol extract, potassium oleate, profenofos, psorale, prothiofos, pyrazophos, pyrethrin, pyridaphene, Pyrimidin, pyriproxyfen, extract of saponin, saponin, rapeseed oil, rotenone, saponin, saponozit, sodium compound, sodium fluoroantimonate, starch, nematode, streptomyces, flubendiles Amine, sulfur, butyl pyrimidine, tefluthrin, dithiphos, tetrachlorine, chlorpyrifos, methyl ethion, transgenic (eg Cry3Bb1), zodiazepam, Trichoderma, Trichogramma , Sanfulong, Verticillium, cucurbitine, isomeric insecticides (such as κ-bifenin, κ-hetamethrin), mesoionic insecticides, biguanide insecticides, pyrazine fluorene Amine (pyraziflumid); A1) urethanes, including aldicarb, alanycarb, benfuracarb, carbaryl, carbofuran, butyl Carbosulfan, metiocarb, methomyl, oxamyl, pirimicarb, propoxur, and thiodicarb; A2) organic Phosphates, including acephate, azinphos-ethyl, azinphos-methyl, chlorfenvinphos, chlorpyrifos, taosson- Chlorpyrifos-methyl, demeton-S-methyl, diazinon, dichlorvos/DDVP, dicrotophos, damascene Dimethoate), disulfoton, ethion, fenitrothion, fenthion, isoxathion, malathion, methamidaphos, dexamethasone (methidathion), mevinphos, monocrotophos, oxymethoate, oxydemeton-methyl, parathion, parathion-methyl ), phenthoate, phorate, phosalone, phosmet, phosphamidon, armor Pirimiphos-methyl, quinalphos, terbufos, tetrachlorvinphos, triazophos and trichlorfon; A3) cyclopentadiene organic Chlorine compounds, such as endosulfan; A4) non-poor, including ethiprole, fepni, pyrafluprole, and pyriprole; A5) neonicotinoid Analogs, including acetamiprid, cotinine, dinotefuran, imidacloprid, nitenpyram, thiacloprid, and thiamethoxam; A6) promises, such as spinosad and spinetoram; A7) chloride channel activators from statins, including abamectin, indomethacin, ivastatin, Linpitine and methiophene; A8) juvenile hormone mimics such as methoprene ethyl ester, enediyl acetyl ester, methoprene, fenoxycarb and pyriproxyfen; A9 Selective Homoptera feeding blockers, such as pymetrozine, flonicamid, and pyrifluquinazon; A10) 螨 growth inhibitors, such as ketopurine, dexamethasone and acetonide; A11) mitochondrial ATP synthase inhibitors, such as fenfenol, fenbuxil and ruthenium; oxidative phosphorylation decoupling Mixtures, such as chlorfenapyr; A12) nicotinic acetylcholine receptor channel blockers, such as bensultap, captan hydrochloride, thiocyclam, and insecticidal double sodium; A13 a chitin biosynthesis type 0 inhibitor derived from benzamidine, including bistriazole, bifuran, flufenadol, hexaflulon, riffin, novalon, and defolon; A14) Chitin biosynthesis type 1 inhibitors, such as buffingin; A15) quercerin, such as cytosine; A16) quercetin receptor agonists, such as fentanyl, fenfenol, fenfen and ringworm醯肼; A17) octopamine receptor agonist, such as triterpenoids; A18) mitochondrial complex electron transport inhibitors pyridaben, tebufenpyrad, tolfenpyrad, fluoride Fennelin, nitrile pyridoxine, butaflurane, amame pine, hydrazine or pyrimidin; A19) voltage-dependent sodium channel blockers, such as indac (ind Oxcarb) and cyprofenil; A20) inhibitors of lipid synthesis, such as chlorfenapyr, spironolactone and spirotetramat; A21) loranodine receptor modulators from diamines, including flubendiles Diammonium, o-phthalamide compound (R)-3-chloro-N1-{2-methyl-4-[1,2,2,2-tetrafluoro-1-(trifluoromethyl) Phenyl]-N2-(1-methyl-2-methylsulfonylethyl) phthalic acid and (S)-3-chloro-N1-{2-methyl-4-[ 1,2,2,2-tetrafluoro-1-(trifluoromethyl)ethyl]phenyl}-N2-(1-methyl-2-methylsulfonylethyl) phthalic acid , chloranthraniliprole and cyanarylpron; A22) compounds of unknown or indeterminate mode of action, such as azadirachtin, amidoflumet, bifenazate, fluthiazer, Synergistic sperm, acetamiprid, fluramide; or A23) sodium channel regulators from pyrethroids, including analin, pyrethroid, bifenin, cyprofen, λ-sailo Ning, Sai Ningning, α-赛灭宁, β-赛灭宁, ξ-赛灭宁, 灭宁宁, Yihuali, Yifening, Fenpning, Fenhuali, Husaining, τ-Fu Huali, Baishenning, Qifufen And Tai Ning.

殺真菌劑：B0)苯并烯氟菌唑、抗霜黴菌、辛唑嘧菌胺、吲唑磺菌胺、銅鹽(例如氫氧化銅、氯氧化銅、硫酸銅、過氧硫酸銅)、白可列(boscalid)、硫氟醯胺(thiflumazide)、氟替尼(flutianil)、呋霜靈(furalaxyl)、腐絕(thiabendazole)、麥鏽靈(benodanil)、滅普寧(mepronil)、艾索非他米(isofetamid)、甲呋醯胺(fenfuram)、必殺吩(bixafen)、氟唑菌醯胺(fluxapyroxad)、噴福芬(penflufen)、氟唑環菌胺(sedaxane)、丁香菌酯(coumoxystrobin)、烯肟菌酯(enoxastrobin)、氟菌蟎酯(flufenoxystrobin)、唑菌酯(pyraoxystrobin)、唑胺菌酯(pyrametostrobin)、三環吡菌威(triclopyricarb)、烯肟菌胺(fenaminstrobin)、苯氧菌胺(metominostrobin)、吡菌苯威(pyribencarb)、敵蟎普(meptyldinocap)、三苯醋錫(fentin acetate)、氯化三苯錫(fentin chloride)、氫氧化三苯錫(fentin hydroxide)、土黴素(oxytetracycline)、克氯得(chlozolinate)、地茂散(chloroneb)、四氯硝基苯(tecnazene)、依得利(etridiazole)、霜黴威(iodocarb)、硫菌威(prothiocarb)、枯草芽孢桿菌合成、解澱粉芽孢桿菌(例如菌株QST 713、FZB24、MBI600、D747)、來自互生葉白千層(Melaleuca alternifolia)之提取物、來自白羽扇豆(Lupinus albus doce)之提取物、BLAD多肽、啶菌噁唑(pyrisoxazole)、噁咪唑(oxpoconazole)、乙環唑(etaconazole)、胺苯吡菌酮(fenpyrazamine)、萘替芬(naftifine)、特比萘芬(terbinafine)、維利微素(validamycin)、丁吡嗎啉(pyrimorph)、威利芬那雷特(valifenalate)、熱必斯(fthalide)、撲殺熱(probenazole)、異噻菌胺(isotianil)、昆布糖(laminarin)、來自大虎杖之提取物、亞磷酸及鹽、葉枯酞(teclofthalam)、咪唑嗪(triazoxide)、甲氧苯唳菌(pyriofenone)、有機油、碳酸氫鉀、四氯異苯腈、唑呋草(fluoroimide)；B1)唑類，包括比多農、糠菌唑、環克座、待克利、達克利、恩康唑、依普座、氟喹唑、芬克座、護矽得、護汰芬、菲克 利、亞胺唑、種菌唑、滅特座、邁克尼、平克座、普克利、丙硫菌唑、矽氟唑、三泰芬、三泰隆、得克利、四克利、滅菌唑、咪醯胺、披扶座、依滅列、賽福座、賽座滅、免賴得、貝芬替、腐絕、麥穗靈、噻唑菌胺、依得利及殺紋寧、氧環唑、達克利-M、噁咪唑、巴克素、烯效唑、1-(4-氯-苯基)-2-([1,2,4]***-1-基)-環庚醇及硫酸依滅列；B2)嗜毬果傘素，包括亞托敏、醚菌胺、烯肟菌酯、氟嘧菌酯、克收欣(kresoxim-methyl)、苯氧菌胺、肟醚菌胺、啶氧菌酯、百克敏、三氟敏、烯肟菌酯、(2-氯-5-[1-(3-甲基苄氧亞胺基)乙基]苄基)胺基甲酸甲酯、(2-氯-5-[1-(6-甲基吡啶-2-基甲氧亞胺基)乙基]苄基)胺基甲酸甲酯及2-(鄰-(2,5-二甲基苯氧亞甲基)-苯基)-3-甲氧基丙烯酸甲酯、2-(2-(6-(3-氯-2-甲基-苯氧基)-5-氟-嘧啶-4-基氧基)-苯基)-2-甲氧亞胺基-N-甲基-乙醯胺及3-甲氧基-2-(2-(N-(4-甲氧基-苯基)-環丙烷伸亞胺醯基硫基甲基)-苯基)-丙烯酸甲酯；B3)羧醯胺，包括萎鏽靈、本達樂(benalaxyl)、本達樂-M、環醯菌胺、福多寧(flutolanil)、福拉比(furametpyr)、滅普寧(mepronil)、滅達樂(metalaxyl)、右滅達樂(mefenoxam)、呋醯胺、歐殺斯(oxadixyl)、嘉保信(oxycarboxin)、吡噻菌胺、吡唑萘菌胺、賽氟滅(thifluzamide)、汰敵寧(tiadinil)、3,4-二氯-N-(2-氰基苯基)異噻唑-5-甲醯胺、達滅芬(dimethomorph)、氟嗎啉、氟醯菌胺、氟吡菌胺(吡考苯紮(picobenzamid))、座賽胺、加普胺、二氯西莫、雙炔醯菌胺、N-(2-(4-[3-(4-氯苯基)丙-2-炔氧基]-3-甲氧苯基)乙基)-2-甲磺醯基-胺基-3-甲基丁醯胺、N-(2-(4-[3-(4-氯-苯基)丙-2-炔氧基]-3-甲氧基-苯基)乙基)-2-乙磺醯胺基-3-甲基丁醯胺、3-(4-氯苯基)-3-(2-異丙氧羰基-胺基-3-甲基-丁醯胺基)丙酸甲酯、N-(4'-溴聯苯-2-基)-4-二氟甲基-甲基噻唑-δ-甲醯胺、N-(4'-三氟甲基-聯苯-2-基)-4-二氟甲基-2-甲基噻唑-5-甲醯胺、N-(4'-氯-3'-氟聯苯-2-基)-4-二氟甲基-2-甲基-噻唑-5-甲醯胺、N-(3\4'-二氯-4-氟聯苯-2-基)-3-二氟 -甲基-1-甲基-吡唑-4-甲醯胺、N-(3',4'-二氯-5-氟聯苯-2-基)-3-二氟甲基-1-甲基吡唑-4-甲醯胺、N-(2-氰基-苯基)-3,4-二氯異噻唑-5-甲醯胺、2-胺基-4-甲基-噻唑-5-甲醯苯胺、2-氯-N-(1,1,3-三甲基-茚滿-4-基)-菸鹼醯胺、N-(2-(1,3-二甲基丁基)-苯基)-1,3-二甲基-5-氟-1H-吡唑-4-甲醯胺、N-(4'-氯-3',5-二氟-聯苯-2-基)-3-二氟甲基-1-甲基-1H-吡唑-4-甲醯胺、N-(4'-氯-3',5-二氟-聯苯-2-基)-3-三氟甲基-1-甲基-1H-吡唑-4-甲醯胺、N-(3',4'-二氯-5-氟-聯苯-2-基)-3-三氟甲基-1-甲基-1H-吡唑-4-甲醯胺、N-(3',5-二氟-4'-甲基-聯苯-2-基)-3-二氟甲基-1-甲基-1H-吡唑-4-甲醯胺、N-(3',5-二氟-4'-甲基-聯苯-2-基)-3-三氟甲基-1-甲基-1H-吡唑-4-甲醯胺、N-(順-2-雙環丙基-2-基-苯基)-3-二氟甲基-1-甲基-1H-吡唑-4-甲醯胺、N-(反-2-雙環丙基-2-基-苯基)-3-二氟-甲基-1-甲基-1H-吡唑-4-甲醯胺、氟吡菌醯胺、N-(3-乙基-3,5-5-三甲基-環己基)-3-甲醯胺基-2-羥基-苯甲醯胺、土黴素、矽硫芬、N-(6-甲氧基-吡啶-3-基)環丙烷甲醯胺、2-碘-N-苯基-苯甲醯胺、N-(2-雙環-丙基-2-基-苯基)-3-二氟甲基-1-甲基吡唑-4-基甲醯胺、N-(3',4',5'-三氟聯苯-2-基)-1,3-二甲基吡唑-4-基甲醯胺、N-(3',4',5'-三氟聯苯-2-基)-1,3-二甲基-5-氟吡唑-4-基-甲醯胺、N-(3',4',5'-三氟聯苯-2-基)-5-氯-1,3-二甲基-吡唑-4-基甲醯胺、N-(3',4',5'-三氟聯苯-2-基)-3-氟甲基-1-甲基吡唑-4-基甲醯胺、N-(3',4',5'-三氟聯苯-2-基)-3-(氯氟甲基)-1-甲基吡唑-4-基甲醯胺、N-(3',4',5'-三氟聯苯-2-基)-3-二氟甲基-1-甲基吡唑-4-基甲醯胺、N-(3',4',5'-三氟聯苯-2-基)-3-二氟甲基-5-氟-1-甲基吡唑-4-基甲醯胺、N-(3',4',5'-三氟聯苯-2-基)-5-氯-3-二氟甲基-1-甲基吡唑-4-基甲醯胺、N-(3',4',5'-三氟聯苯-2-基)-3-(氯二氟甲基)-1-甲基吡唑-4-基甲醯胺、N-(3',4',5'-三氟聯苯-2-基)-1-甲基-3-三氟甲基吡唑-4-基甲醯胺、N-(3',4',5'-三氟聯苯-2-基)-5-氟-1-甲基-3-三氟甲基吡唑-4-基甲醯胺、N-(3',4',5'-三氟聯苯-2-基)-5-氯-1-甲基-3-三氟甲基吡唑-4-基甲醯 胺、N-(2',4',5'-三氟聯苯-2-基)-1,3-二甲基吡唑-4-基甲醯胺、N-(2',4',5'-三氟聯苯-2-基)-1,3-二甲基-5-氟吡唑-4-基甲醯胺、N-(2',4',5'-三氟聯苯-2-基)-5-氯-1,3-二甲基吡唑-4-基甲醯胺、N-(2',4',5'-三氟聯苯-2-基)-3-氟甲基-1-甲基吡唑-4-基甲醯胺、N-(2',4',5'-三氟聯苯-2-基)-3-(氯氟甲基)-1-甲基吡唑-4-基甲醯胺、N-(2',4',5'-三氟聯苯-2-基)-3-二氟甲基-1-甲基吡唑-4-基甲醯胺、N-(2',4',5'-三氟聯苯-2-基)-3-二氟甲基-5-氟-1-甲基吡唑-4-基甲醯胺、N-(2',4',5'-三氟聯苯-2-基)-5-氯-3-二氟甲基-1-甲基吡唑-4-基甲醯胺、N-(2',4',5'-三氟聯苯-2-基)-3-(氯二氟甲基)-1-甲基吡唑-4-基甲醯胺、N-(2',4',5'-三氟聯苯-2-基)-1-甲基-3-三氟甲基吡唑-4-基甲醯胺、N-(2',4',5'-三氟聯苯-2-基)-5-氟-1-甲基-3-三氟甲基吡唑-4-基甲醯胺、N-(2',4',5'-三氟聯苯-2-基)-5-氯-1-甲基-3-三氟甲基吡唑-4-基甲醯胺、N-(3',4'-二氯-3-氟聯苯-2-基)-1-甲基-3-三氟甲基-1H-吡唑-4-甲醯胺、N-(3',4'-二氯-3-氟聯苯-2-基)-1-甲基-3-二氟甲基-1H-吡唑-4-甲醯胺、N-(3',4'-二氟-3-氟聯苯-2-基)-1-甲基-3-三氟甲基-1H-吡唑-4-甲醯胺、N-(3',4'-二氟-3-氟聯苯-2-基)-1-甲基-S-二氟甲基-1H-吡唑-4-甲醯胺、N-(3'-氯-4'-氟-3-氟聯苯-2-基)-1-甲基-3-二氟甲基-1H-吡唑-4-甲醯胺、N-(3',4'-二氯-4-氟聯苯-2-基)-1-甲基-3-三氟甲基-1H-吡唑-4-甲醯胺、N-(3',4'-二氟-4-氟聯苯-2-基)-1-甲基-S-三氟甲基-1H-吡唑-4-甲醯胺、N-(3',4'-二氯-4-氟聯苯-2-基)-1-甲基-3-二氟甲基-1H-吡唑-4-甲醯胺、N-(3',4'-二氟-4-氟聯苯-2-基)-1-甲基-3-二氟甲基-1H-吡唑-4-甲醯胺、N-(3'-氯-4'-氟-4-氟聯苯-2-基)-1-甲基-S-二氟甲基-1H-吡唑-4-甲醯胺、N-(3',4'-二氯-5-氟聯苯-2-基)-1-甲基-3-三氟甲基-1H-吡唑-4-甲醯胺、N-(3',4'-二氟-5-氟聯苯-2-基)-1-甲基-3-三氟甲基-1H-吡唑-4-甲醯胺、N-(3',4'-二氯-5-氟聯苯-2-基)-1-甲基-S-二氟甲基-1H-吡唑-甲醯胺、N-(3',4'-二氟-5-氟聯苯-2-基)-1-甲基-3-二氟甲基-1H-吡唑-4-甲醯胺、N-(3',4'-二氯-5-氟聯苯-2-基)- 1,3-二甲基-1H-吡唑-4-甲醯胺、N-(3'-氯-4'-氟-5-氟聯苯-2-基)-1-甲基-3-二氟甲基-1H-吡唑-4-甲醯胺、N-(4'-氟-4-氟聯苯-2-基)-1-甲基-3-三氟甲基-1H-吡唑-4-甲醯胺、N-(4'-氟-5-氟聯苯-2-基)-1-甲基-3-三氟甲基-1H-吡唑-4-甲醯胺、N-(4'-氯-5-氟聯苯-2-基)-1-甲基-3-三氟甲基-1H-吡唑-4-甲醯胺、N-(4'-甲基-5-氟聯苯-2-基)-1-甲基-3-三氟甲基-1H-吡唑-4-甲醯胺、N-(4'-氟-5-氟聯苯-2-基)-1,3-二甲基-1H-吡唑-4-甲醯胺、N-(4'-氯-5-氟聯苯-2-基)-1,3-二甲基-1H-吡唑-4-甲醯胺、N-(4'-甲基-5-氟聯苯-2-基)-1,3-二甲基-1H-吡唑-4-甲醯胺、N-(4'-氟-6-氟聯苯-2-基)-1-甲基-3-三氟甲基-1H-吡唑-4-甲醯胺、N-(4'-氯-6-氟聯苯-2-基)-1-甲基-3-三氟甲基-1H-吡唑-4-甲醯胺、N-[2-(1,1,2,3,3,3-六氟丙氧基)-苯基]-3-二氟甲基-1-甲基-1H-吡唑-4-甲醯胺、N-[4'-(三氟甲硫基)-聯苯-2-基]-3-二氟甲基-1-甲基-1H-吡唑-4-甲醯胺及N-[4'-(三氟甲硫基)-聯苯-2-基]-1-甲基-3-三氟甲基-1-甲基-1H-吡唑-4-甲醯胺；B4)雜環化合物，包括扶吉胺、比芬諾(pyrifenox)、布瑞莫(bupirimate)、嘧菌環胺、芬瑞莫(fenarimol)、富米綜(ferimzone)、嘧菌胺、尼瑞莫(nuarimol)、派美尼(pyrimethanil)、賽福寧(triforine)、拌種咯、護汰寧、阿迪嗎啉(aldimorph)、嗎菌靈、芬普福、三得芬、苯鏽啶、依普同、撲滅寧、免克寧、凡殺同、咪唑菌酮、辛噻酮、撲殺熱、5-氯-7-(4-甲基-哌啶-1-基)-6-(2,4,6-三氟苯基)-[1,2,4]***并[1,5-a]嘧啶、敵菌靈、達滅淨、百快隆、丙氧喹啉、三賽唑、2-丁氧基-6-碘-3-丙基烯-4-酮、酸化苯并噻二唑-S-甲酯、四氯丹、蓋普丹(captan)、邁隆、福爾培(folpet)、氰菌胺、快諾芬(quinoxyfen)、N,N-二甲基-3-(3-溴-6-氟-2-甲基吲哚-1-磺醯基)-[1,2,4]***-1-磺醯胺、5-乙基-6-辛基-[1,2,4]***并[1,5-a]嘧啶-2,7-二胺、2,3,5,6-四氯-4-甲磺醯基-吡啶、3,4,5-三氯-吡啶-2,6-二甲腈、N-(1-(5-溴-3-氯-吡啶-2-基)-乙基)-2,4-二氯-菸鹼醯胺、N-((5-溴-3-氯吡啶-2-基)-甲基)-2,4-二氯- 菸鹼醯胺、二氟林、氯啶、嗎菌靈乙酸酯、唑呋草、殺稻瘟菌素-S、蟎離丹、咪菌威、野燕枯、野燕枯-甲基硫酸鹽、歐索林酸及粉病靈；B5)胺基甲酸酯，包括鋅錳乃浦、錳乃浦、威百畝、磺菌威、免得爛、福美鐵、甲基鋅乃浦、得恩地、鋅乃浦、益穗、乙黴威、纈黴威、苯噻菌胺、霜黴威、霜黴威鹽酸鹽、N-(1-(1-(4-氰基苯基)-乙磺醯基)丁-2-基)胺基甲酸4-氟苯酯、3-(4-氯-苯基)-3-(2-異丙氧基羰胺基-3-甲基-丁醯基胺基)丙酸甲酯；或B6)其他殺真菌劑，包括胍、多寧、多寧游離鹼、克熱淨、雙胍鹽；抗生素：春日黴素、土黴素及其鹽、鏈黴素、保粒黴素、維利黴素A；硝基苯基衍生物：百蟎克、白粉克、大脫蟎；含硫雜環基化合物：腈硫醌、稻瘟靈；有機金屬化合物：薯瘟錫鹽；有機磷化合物：護粒松、丙基喜樂松、福賽得(fosetyl)、福賽得-鋁、亞磷酸及其鹽、白粉松、甲基脫克松；有機氯化合物：益發靈、氟硫滅、六氯苯、苯酞、賓克隆、五氯硝基苯、多保淨、甲基多保淨、甲基益發靈；其他：環氟菌胺、克絕、二甲嘧酚、依瑞莫、呋霜靈、滅芬農及螺環菌胺、雙胍辛乙酸鹽、克熱淨乙酸鹽、克熱淨-參(烷苯磺酸鹽)、春日黴素鹽酸鹽水合物、二氯酚、五氯苯酚及其鹽、N-(4-氯-2-硝基-苯基)-N-乙基-4-甲基-苯磺醯胺、氯硝胺、異丙基滅鏽胺、四氯硝基苯、聯苯、溴硝丙二醇、二苯胺、米多黴素、啉銅、調環酸鈣、N-(環丙基甲氧基亞胺基-(6-二氟甲氧基-2,3-二氟-苯基)-甲基)-2-苯基乙醯胺、N'-(4-(4-氯-3-三氟甲基-苯氧基)-2,5-二甲基-苯基)-N-乙基-N-甲基甲脒、N'-(4-(4-氟-3-三氟甲基-苯氧基)-2,5-二甲基-苯基)-N-乙基-N-甲基甲脒、N'-(2-甲基-5-三氟甲基-4-(3-三甲基矽烷基-丙氧基)-苯基)-N-乙基-N-甲基甲脒及N'-(5-二氟甲基-2-甲基-4-(3-三甲基矽烷基-丙氧基)-苯基)-N-乙基-N-甲基甲脒。 Fungicide: B0) Benzoene fluconazole, downy mildew, fluzinamide, carbazolam, copper salt (such as copper hydroxide, copper oxychloride, copper sulfate, copper peroxosulfate), Boscalid, thiflumazide, flutianil, furalaxyl, thiabendazole, benodanil, mepronil, Aesop Isofetamid, fenfuram, bixafen, fluxapyroxad, penflufen, sedaxane, syringolide (isofetamid) Coumoxystrobin), enoxastrobin, flufenoxystrobin, pyraoxystrobin, pyramatetostrobin, triclopyricarb, fenaminstrobin , metominostrobin, pyribencarb, meptyldinocap, fentin acetate, fentin chloride, triphenyltin hydroxide (fentin) Hydroxide), oxytetracycline, chlozolinate, chloroneb, tetrachloronitrobenzene (tecnaze) Ne), etridiazole, iodocarb, prothiocarb, Bacillus subtilis synthesis, Bacillus amyloliquefaciens (eg strain QST 713, FZB24, MBI600, D747), from alternate leaf white Extract of Melaleuca alternifolia , extract from white lupinus ( Lupinus albus doce ), BLAD polypeptide, pyrisoxazole, oxpoconazole, etaconazole, albendazole Fenpyrazamine, naftifine, terbinafine, validamycin, pyrimorph, valifenalate, philistine (fthalide), probenazole, isotianil, laminarin, extract from Polygonum cuspidatum, phosphorous acid and salt, teclofthalam, triazoxide, nail Pyrofenone, organic oil, potassium bicarbonate, tetrachloroisophthalonitrile, fluoroimide; B1) azoles, including more than peas, carbendazole, cyclazone, holly, dar Klee, Enconazole, Epto, Fluoroquinazole, Fenke, Guardian, and Protection , Fickley, imidazole, inocastazole, chlorpyrifos, micney, pingke, pugliine, prothioconazole, flurazol, tripafin, three tailong, dexclily, tikili, sterilized azole, Imiamine, pedicure, idylindole, safty, squadron, smectite, befenbut, rot, wheat ear, ethaboxam, edulis and chlorpyrifos , Dakli-M, Imidazole, Barkerol, Uniconazole, 1-(4-Chloro-phenyl)-2-([1,2,4]triazol-1-yl)-cycloheptanol and sulfuric acid B2) opiate, including atorenosin, noxylamine, enestrobin, fluoxastrobin, kresoxim-methyl, phenoxybenzamine, oxime, Pyridoxine, hexagram, trifluoro-sensitive, enestrobin, methyl 2-(2-chloro-5-[1-(3-methylbenzyloxyimino)ethyl]benzyl)carbamate, Methyl 2-chloro-5-[1-(6-methylpyridin-2-ylmethoxyimino)ethyl]benzyl)carbamate and 2-(o-(2,5-dimethyl) Methyl phenoxymethylene)-phenyl)-3-methoxy acrylate, 2-(2-(6-(3-chloro-2-methyl-phenoxy)-5-fluoro-pyrimidine- 4-yloxy)-phenyl)-2-methoxyimino-N-methyl-acetamide and 3-methoxy-2- (2-(N-(4-methoxy-phenyl)-cyclopropane)-imidylthiomethyl)-phenyl)-methyl acrylate; B3) Carboxamide, including wilting, present Benalaxyl, Bendula-M, cycloheximide, flutanil, furametpyr, mepronil, metalaxyl, mefenoxam ), furosemide, oxadixyl, oxycarboxin, penthiopyre, pyrazolamide, thifluzamide, tiadinil, 3,4-dichloro -N-(2-cyanophenyl)isothiazol-5-formamide, dimethomorph, flumorph, flufenamide, fluoprazamide (picobenzamid), Amine, gupamine, diclosan, dipropionamide, N-(2-(4-[3-(4-chlorophenyl)prop-2-ynyloxy)-3-methoxy Phenyl)ethyl)-2-methanesulfonyl-amino-3-methylbutanamine, N-(2-(4-[3-(4-chloro-phenyl)prop-2-ynyloxy) 3-yloxy-phenyl)ethyl)-2-ethanesulfonylamino-3-methylbutanamine, 3-(4-chlorophenyl)-3-(2-isopropoxy Carbonyl-amino-3-methyl-butylammonium)propionic acid methyl ester, N-(4'-bromobiphenyl-2-yl)-4-difluoromethyl-methylthiazole-δ-formamidine amine N-(4'-trifluoromethyl-biphenyl-2-yl)-4-difluoromethyl-2-methylthiazole-5-formamide, N-(4'-chloro-3'-fluoro Biphenyl-2-yl)-4-difluoromethyl-2-methyl-thiazole-5-carboxamide, N-(3\4'-dichloro-4-fluorobiphenyl-2-yl)- 3-Difluoro-methyl-1-methyl-pyrazole-4-carboxamide, N-(3',4'-dichloro-5-fluorobiphenyl-2-yl)-3-difluoromethyl 1-methylpyrazole-4-carboxamide, N-(2-cyano-phenyl)-3,4-dichloroisothiazol-5-carboxamide, 2-amino-4-methyl Base-thiazole-5-methylanilide, 2-chloro-N-(1,1,3-trimethyl-indan-4-yl)-nicotinium amide, N-(2-(1,3- Dimethylbutyl)-phenyl)-1,3-dimethyl-5-fluoro-1H-pyrazole-4-carboxamide, N-(4'-chloro-3',5-difluoro- Biphenyl-2-yl)-3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, N-(4'-chloro-3',5-difluoro-biphenyl- 2-yl)-3-trifluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, N-(3',4'-dichloro-5-fluoro-biphenyl-2-yl )-3-trifluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, N-(3',5-difluoro-4'-methyl-biphenyl-2-yl)- 3-Difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, N-(3',5-difluoro-4'-methyl-biphenyl-2-yl)-3- Trifluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, N-(cis-2-bicyclopropyl-2-yl-benzene )-3-Difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, N-(trans-2-dicyclopropyl-2-yl-phenyl)-3-difluoro-A -1-methyl-1H-pyrazole-4-carboxamide, flupirtine, N-(3-ethyl-3,5-5-trimethyl-cyclohexyl)-3-carboxamidine Amino-2-hydroxy-benzamide, oxytetracycline, thiophanate, N-(6-methoxy-pyridin-3-yl)cyclopropanecarbamide, 2-iodo-N-phenyl- Benzylamine, N-(2-bicyclo-propyl-2-yl-phenyl)-3-difluoromethyl-1-methylpyrazol-4-ylcarboxamide, N-(3', 4',5'-Trifluorobiphenyl-2-yl)-1,3-dimethylpyrazole-4-ylcarboxamide, N-(3',4',5'-trifluorobiphenyl- 2-yl)-1,3-dimethyl-5-fluoropyrazole-4-yl-carbenamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-5 -Chloro-1,3-dimethyl-pyrazol-4-ylcarboxamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-3-fluoromethyl-1 -methylpyrazol-4-ylformamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-3-(chlorofluoromethyl)-1-methylpyrazole 4-ylformamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-3-difluoromethyl-1-methylpyrazole-4-ylcarboxamide , N-(3',4',5'-trifluorobiphenyl-2-yl)-3-difluoromethyl-5-fluoro-1-methylpyrazole-4-ylcarboxamide, N- (3',4',5'-trifluorobiphenyl-2-yl)-5-chloro-3-di Fluoromethyl-1-methylpyrazole-4-ylcarboxamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-3-(chlorodifluoromethyl)- 1-methylpyrazol-4-ylcarboxamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-1-methyl-3-trifluoromethylpyrazole- 4-ylformamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-5-fluoro-1-methyl-3-trifluoromethylpyrazol-4-yl Formamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-5-chloro-1-methyl-3-trifluoromethylpyrazole-4-ylcarboxamide , N-(2',4',5'-trifluorobiphenyl-2-yl)-1,3-dimethylpyrazol-4-ylcarboxamide, N-(2',4',5 '-Trifluorobiphenyl-2-yl)-1,3-dimethyl-5-fluoropyrazole-4-ylcarboxamide, N-(2',4',5'-trifluorobiphenyl- 2-yl)-5-chloro-1,3-dimethylpyrazole-4-ylcarboxamide, N-(2',4',5'-trifluorobiphenyl-2-yl)-3- Fluoromethyl-1-methylpyrazole-4-ylcarboxamide, N-(2',4',5'-trifluorobiphenyl-2-yl)-3-(chlorofluoromethyl)-1 -methylpyrazol-4-ylcarboxamide, N-(2',4',5'-trifluorobiphenyl-2-yl)-3-difluoromethyl-1-methylpyrazole-4 -carbamamine, N-(2',4',5'-trifluorobiphenyl-2-yl)-3-difluoromethyl-5-fluoro-1-methylpyrazole-4-yl Indoleamine, N-(2',4',5'-trifluorobiphenyl-2-yl)-5-chloro-3-difluoromethyl-1-methylpyrazol-4-ylcarboxamide, N-(2',4',5'-trifluorobiphenyl-2-yl)-3-(chlorodifluoromethyl)-1-methylpyrazol-4-ylcarboxamide, N-(2 ',4',5'-Trifluorobiphenyl-2-yl)-1-methyl-3-trifluoromethylpyrazole-4-ylcarboxamide, N-(2',4',5' -Trifluorobiphenyl-2-yl)-5-fluoro-1-methyl-3-trifluoromethylpyrazole-4-ylcarboxamide, N-(2',4',5'-trifluoro Biphenyl-2-yl)-5-chloro-1-methyl-3-trifluoromethylpyrazole-4-ylcarboxamide, N-(3',4'-dichloro-3-fluorobiphenyl -2-yl)-1-methyl-3-trifluoromethyl-1H-pyrazole-4-carboxamide, N-(3',4'-dichloro-3-fluorobiphenyl-2-yl )-1-methyl-3-difluoromethyl-1H-pyrazole-4-carboxamide, N-(3',4'-difluoro-3-fluorobiphenyl-2-yl)-1- Methyl-3-trifluoromethyl-1H-pyrazole-4-carboxamide, N-(3',4'-difluoro-3-fluorobiphenyl-2-yl)-1-methyl-S -difluoromethyl-1H-pyrazole-4-carboxamide, N-(3'-chloro-4'-fluoro-3-fluorobiphenyl-2-yl)-1-methyl-3-difluoro Methyl-1H-pyrazole-4-carboxamide, N-(3',4'-dichloro-4-fluorobiphenyl-2-yl)-1-methyl-3-trifluoromethyl-1H -pyrazole-4-carbamide, N-(3',4'-difluoro-4-fluorobiphenyl-2-yl)-1-methyl-S-trifluoromethyl-1H-pyrazole- 4-Protonamine, N-(3',4'-dichloro-4-fluorobiphenyl-2-yl)-1-methyl-3-difluoromethyl-1H-pyrazole-4-carboxamidine Amine, N- (3',4'-Difluoro-4-fluorobiphenyl-2-yl)-1-methyl-3-difluoromethyl-1H-pyrazole-4-carboxamide, N-(3'- Chloro-4'-fluoro-4-fluorobiphenyl-2-yl)-1-methyl-S-difluoromethyl-1H-pyrazole-4-carboxamide, N-(3',4'- Dichloro-5-fluorobiphenyl-2-yl)-1-methyl-3-trifluoromethyl-1H-pyrazole-4-carboxamide, N-(3',4'-difluoro-5 -fluorobiphenyl-2-yl)-1-methyl-3-trifluoromethyl-1H-pyrazole-4-carboxamide, N-(3',4'-dichloro-5-fluorobiphenyl -2-yl)-1-methyl-S-difluoromethyl-1H-pyrazole-formamide, N-(3',4'-difluoro-5-fluorobiphenyl-2-yl)- 1-methyl-3-difluoromethyl-1H-pyrazole-4-carboxamide, N-(3',4'-dichloro-5-fluorobiphenyl-2-yl)-1,3- Dimethyl-1H-pyrazole-4-carboxamide, N-(3'-chloro-4'-fluoro-5-fluorobiphenyl-2-yl)-1-methyl-3-difluoromethyl -1H-pyrazole-4-carbamide, N-(4'-fluoro-4-fluorobiphenyl-2-yl)-1-methyl-3-trifluoromethyl-1H-pyrazole-4- Formamide, N-(4'-fluoro-5-fluorobiphenyl-2-yl)-1-methyl-3-trifluoromethyl-1H-pyrazole-4-carboxamide, N-(4 '-Chloro-5-fluorobiphenyl-2-yl)-1-methyl-3-trifluoromethyl-1H-pyrazole-4-carboxamide, N-(4'-methyl-5-fluoro Biphenyl-2-yl)-1-methyl-3-trifluoromethyl-1H-pyrazole-4-carboxamide, N-(4'-fluoro-5-fluorobiphenyl-2-yl)- 1,3-two Methyl-1H-pyrazole-4-carboxamide, N-(4'-chloro-5-fluorobiphenyl-2-yl)-1,3-dimethyl-1H-pyrazole-4-carboxamidine Amine, N-(4'-methyl-5-fluorobiphenyl-2-yl)-1,3-dimethyl-1H-pyrazole-4-carboxamide, N-(4'-fluoro-6 -fluorobiphenyl-2-yl)-1-methyl-3-trifluoromethyl-1H-pyrazole-4-carboxamide, N-(4'-chloro-6-fluorobiphenyl-2-yl )-1-methyl-3-trifluoromethyl-1H-pyrazole-4-carboxamide, N-[2-(1,1,2,3,3,3-hexafluoropropoxy)- Phenyl]-3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, N-[4'-(trifluoromethylthio)-biphenyl-2-yl]-3 -difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide and N-[4'-(trifluoromethylsulfanyl)-biphenyl-2-yl]-1-methyl-3 -trifluoromethyl-1-methyl-1H-pyrazole-4-carboxamide; B4) heterocyclic compounds including chloramine, pyrifenox, bupirimate, azoxystrobin Amine, fenarimol, ferimzone, azoxystrobin, nuarimol, pyrimethanil, triforine, seed dressing, protection, adi Morphine (aldimorph), carbendazim, fenflufen, triptan, fenpropidin, yiputong, chlorhexidine, chlorhexidine, chlorhexidine, imidacloprid, thiophenone, flutter Pyrogen, 5-chloro-7-(4-methyl-piperidin-1-yl)-6-(2,4,6-trifluorophenyl)-[1,2,4]triazolo[1 , 5-a] pyrimidine, carbendazim, chlorhexidine, baiweilong, propoxyquinoline, trioxazole, 2-butoxy-6-iodo-3-propyl Ace-4-one, acidified benzothiadiazole-S-methyl ester, tetrachlorodan, captan, melon, folfet, cyanamide, quinoxyfen, N , N-dimethyl-3-(3-bromo-6-fluoro-2-methylindole-1-sulfonyl)-[1,2,4]triazole-1-sulfonamide, 5- Ethyl-6-octyl-[1,2,4]triazolo[1,5-a]pyrimidine-2,7-diamine, 2,3,5,6-tetrachloro-4-methylsulfonate Base-pyridine, 3,4,5-trichloro-pyridine-2,6-dicarbonitrile, N-(1-(5-bromo-3-chloro-pyridin-2-yl)-ethyl)-2, 4-Dichloro-nicotine decylamine, N-((5-bromo-3-chloropyridin-2-yl)-methyl)-2,4-dichloro-nicotinamide, difluoroline, chloridine , carbendazim acetate, oxazolin, blasticidin-S, saponin, imiprozil, wild swallow, wild swallow-methyl sulfate, oxolinic acid and powdery disease; B5) urethanes, including zinc-manganese, manganese-niobium, Weibaimu, sulfavir, elixirs, fumei, methyl zinc, Pu'er, zinc, Pu, Yisui, and B. Granules, carbendazim, phenothiamine, propamocarb, chlorfenapyr hydrochloride, N-(1-(1-(4-cyanophenyl)-ethanesulfonyl)butan-2-yl) 4-fluorophenyl carbamic acid, 3-(4-chloro-phenyl)-3-(2-isopropoxycarbonylamino) Methyl 3-methyl-butyl-decylamino)propionate; or B6) other fungicides, including guanidine, tannin, toxin free base, ke shui, biguanide; antibiotics: kasugamycin, oxytetracycline And its salt, streptomycin, granulin, vilimycin A; nitrophenyl derivatives: Baiqi grams, white powder grams, large aphid; sulfur-containing heterocyclic compounds: nitrile sulfonium, rice blast Ling; organometallic compounds: dip and tin salts; organophosphorus compounds: granules pine, propyl chelsson, fosetyl, foss-aluminum, phosphorous acid and its salts, white pine, methyl gram Pine; organochlorine compounds: Yifaling, fluorosulfuron, hexachlorobenzene, benzoquinone, berberine, pentachloronitrobenzene, chlorhexidine, methyl chlorhexidine, methyl valproate; others: cycloflufenamide , ketone, xylenol, irilimole, furosemide, fenfenin and spirocycline, bisindole acetate, gramacetal acetate, gram-net-paraben (alkylbenzene sulfonate), Kasugamycin hydrochloride hydrate, dichlorophenol, pentachlorophenol and its salt, N-(4-chloro-2-nitro-phenyl)-N-ethyl-4-methyl-benzenesulfonamide, Chloramine, isopropyl rustamine, tetrachloronitrate Benzene, biphenyl, bromide, diphenylamine, midodinomycin, copper porphyrin, calcium cyclate, N-(cyclopropylmethoxyimino-(6-difluoromethoxy-2,3) -difluoro-phenyl)-methyl)-2-phenylacetamide, N'-(4-(4-chloro-3-trifluoromethyl-phenoxy)-2,5-dimethyl -phenyl)-N-ethyl-N-methylformamidine, N'-(4-(4-fluoro-3-trifluoromethyl-phenoxy)-2,5-dimethyl-phenyl )-N-ethyl-N-methylformamidine, N'-(2-methyl-5-trifluoromethyl-4-(3-trimethyldecyl-propoxy)-phenyl)- N-ethyl-N-methylformamidine and N'-(5-difluoromethyl-2-methyl-4-(3-trimethyldecyl-propoxy)-phenyl)-N- Ethyl-N-methylformamidine.

除草劑：C1)乙醯輔酶A羧化酶抑制劑(ACC)，例如環己烯酮肟醚，諸如禾草滅、克草同、氯丙氧定、環殺草、西殺草、肟草酮、丁 苯草酮、環苯草酮或吡喃草酮；苯氧基苯氧丙酸酯，諸如炔草酯、氰氟草酯、禾草靈、噁唑禾草靈、精噁唑禾草靈、噻唑禾草靈、吡氟禾草靈、精吡氟禾草靈、吡氟氯禾靈、氟吡甲禾靈、精氟吡甲禾靈、異噁草醚、喔草酯、喹禾靈、精喹禾靈或喹禾糠酯；或芳胺基丙酸，諸如麥草氟甲酯或麥草氟異丙酯；C2)乙醯乳酸合成酶抑制劑(ALS)，例如咪唑啉酮，諸如依滅草、滅草喹、甲基咪草酯(咪草酯)、甲氧咪草菸、甲基咪草菸或咪草菸；嘧啶基醚，諸如嘧硫酸、嘧硫鈉、雙草醚鈉、KIH-6127或嘧苯草肟(pyribenzoxym)；磺醯胺，諸如雙氟磺草胺、唑嘧磺草胺或磺草唑胺；或磺醯脲，諸如醯嘧磺隆、四唑嘧磺隆、苄嘧磺隆、氯嘧磺隆、氯磺隆、醚磺隆、環磺隆、胺苯磺隆、乙氧嘧磺隆、嘧啶磺隆、氯吡嘧磺隆、唑吡嘧磺隆、甲磺隆、菸嘧磺隆、氟嘧磺隆、氟磺隆、吡嘧磺隆、碸嘧磺隆、甲嘧磺隆、噻吩磺隆、醚苯磺隆、苯磺隆、氟胺磺隆、三氟甲磺隆、磺醯磺隆、甲醯嘧磺隆或碘甲磺隆；C3)醯胺，例如草毒死(CDAA)、新燕靈、溴丁醯草胺、草克樂(chiorthiamid)、草乃敵、乙苯醯草(etobenzanid(benzchlomet))、噻唑草醯胺、調節膦(fosamin)或庚醯草胺；C4)生長素除草劑，例如吡啶甲酸，諸如畢克草或畢克爛；或2,4-D或草除靈；C5)生長素輸送抑制劑，例如萘草胺或二氟吡隆；C6)類胡蘿蔔素生物合成抑制劑，例如吡草酮、可滅蹤(clomazone/dimethazone)、吡氟草胺、氟咯草酮、氟啶草酮、吡唑特、苄草唑、異噁唑草酮、異噁氯草酮、甲基磺草酮、磺草酮(sulcotrione/chlormesulone)、克草酮(ketospiradox)、呋草酮、達草滅或殺草強；C7)烯醇丙酮醯莽草酸-3-磷酸合成酶抑制劑(EPSPS)，例如草甘膦或草硫膦；C8)麩醯胺酸合成酶抑制劑，例如畢拉草(bilanafos/bialaphos)或草銨膦；C9)脂質生物合成抑制劑，例如醯苯胺，諸如莎稗磷或苯噻草胺；氯代乙醯苯胺，諸如二甲噻草胺、S-二 甲噻草胺、乙草胺、甲草胺、丁草胺、丁烯草胺、乙醯甲草胺、二甲草胺、吡草胺、異丙甲草胺、S-異丙甲草胺、丙草胺、撲草胺、廣草胺、猛殺草、甲氧噻草胺或二甲苯草胺；硫脲，諸如蘇達滅、草滅特、燕麥敵、哌草丹、EPTC、戊草丹、草達滅、克草猛、苄草丹、禾草丹(thiobencarb/benthiocarb)、野麥畏或滅草猛；或呋草黃或氟草磺胺；C10)有絲***抑制劑，例如胺基甲酸酯，諸如黃草靈、長殺草、氯苯胺靈、坪草丹、拿草特(pronamid/propyzamid)、苯胺靈或仲草丹；二硝基苯胺，諸如氟草胺、比達寧、敵樂胺、丁氟消草、氟消草、黃草消、胺硝草、胺基丙氟靈或氟樂靈；吡啶，諸如氟硫草定或噻草定；或抑草磷、敵草索(DCPA)或抑芽丹；C11)原卟啉原IX氧化酶抑制劑，例如二苯基醚，諸如氟鎖草醚、氟鎖草醚鈉、苯草醚、必芬諾(bifenox)、草枯醚(CNP)、氯氟草醚、消草醚、乙羧氟草醚、氟黃胺草醚、氟呋草醚、乳氟禾草靈、除草醚、硝氟草醚或氧氟吩；噁二唑，諸如丙炔噁草酮或惡草靈；環狀醯亞胺，諸如草芬定、布芬草、唑草酮、吲哚酮草酯、氟烯草酸、丙炔氟草胺、氟米丙平、富普巴(flupropacil)、氟噻甲草酯、甲磺草胺或噻二唑胺；或吡唑，諸如ET-751、JV 485或氟氯草胺；C12)光合作用抑制劑，例如敵稗、噠草特或吡啶達醇；苯并噻二嗪酮，諸如本達隆(bentazone)；二硝基苯酚，例如殺草全、地樂酚、地樂酯、特樂酚或DNOC；聯吡啶，諸如莎草快、苯敵快、敵草快或百草枯；脲，諸如氯溴隆、綠麥隆、枯莠隆、噁唑隆、敵草隆、噻二唑隆、非草隆、伏草隆、異丙隆、異惡隆、利穀隆、噻唑隆、滅草唑、吡喃隆、甲氧隆、綠穀隆、草不隆、環草隆或得匍隆；苯酚，諸如溴苯腈或碘苯腈；氯草敏；三嗪，諸如莠滅淨、莠去津、氰草津、敵草淨(desmein)、戊草津(dimethamethryn)、六嗪酮、撲滅通、撲草淨、撲滅津、西瑪津、西草淨、甲氧去草淨、去草淨、特丁津或草達津；三嗪酮，諸如苯嗪草 酮或賽克津；尿嘧啶，諸如除草定、環草定或特草定；或雙胺基甲酸酯，諸如甜菜安或甜菜寧；C13)增效劑，例如環氧乙烷，諸如滅草環；C14)CIS細胞壁合成抑制劑，例如異噁草胺或敵草腈；C15)各種其他除草劑，例如二氯丙酸，諸如茅草枯；二氫苯并呋喃，諸如乙呋草黃；苯乙酸，諸如伐草克(chlorfenac/fenac)；或疊氮津、燕麥靈、地散磷、苯噻隆、氟磺胺草、特克草(buminafos)、特咪唑草、炔草隆、唑草胺、氯草靈、燕麥酯、枯草隆、環庚草醚、苄草隆、環莠隆、環草津、三環噻草胺、二苄基隆(dibenzyluron)、殺草淨、香草隆、草止津、草藻滅、乙嗪草酮、氟唑磺隆、氟苯草滅(fluorbentranil)、氟胺草唑、草特靈、異樂靈、卡草靈、氟磺醯草胺、滅草隆、滅落脫、敵草胺、磺樂靈、噁美酮(oxaciclomefone)、棉胺寧、哌草磷、環丙腈津、環丙氟靈、稗草畏、密草通、草克死(CDEC)、芽根靈、三嗪氟草胺、***羧醯胺(triazofenamid)或三甲隆；或其環境上相容的鹽。 Herbicide: C1) Acetyl-CoA carboxylase inhibitor (ACC), such as cyclohexenone oxime ether, such as grass, ketone, chlorpropoxy, cyclohexane, chlorpyrifos, valerian Ketone, butyl Benzorone, benzophenone or pyridone; phenoxyphenoxypropionate, such as clodinafop, cyhalofop, oxacillin, oxazolyl, oxazolyl Thiazoloxacil, flurazepam, flupirtine, flupirtine, flupirtine, flupirtine, clomazone, valerate, quizalofop, Quinoxaline or quinoline ester; or arylaminopropionic acid, such as chloroacetate or wheat straw fluoroisopropyl; C2) acetamidine lactate synthase inhibitor (ALS), such as imidazolinone, such as Grass, imazaquin, methyl imazethate (imipramide), imazamox, methyl imazethapyr or imazethapyr; pyrimidinyl ether, such as pyruvic acid, sodium pyrithione, sodium bispyribofate, KIH-6127 or pyribenzoxym; sulfonamide, such as diflufenacil, flufensulfuron or sulfazinamide; or sulfonylurea, such as sulfuron-methyl, tetrazosulfuron-methyl , bensulfuron-methyl, chlorsulfuron-methyl, chlorsulfuron-methyl, ethersulfuron-methyl, sulfonate, amphetsulfuron, ethoxysulfuron, pyrimidine, chlorpyrifos, oxazolsulfuron, Metsulfuron, nicosulfuron, flusulfuron, flusulfuron, Sulfoxsulfuron, sulfuron-methyl, sulfometuron, thifensulfuron, ethersulfuron, tribenuron, flucarbazone, trifluoromethanesulfuron, sulfonium, sulfometuron or iodine Methotrexate; C3) indoleamines, such as chlorpyrifos (CDAA), neoventry, bromobutachlor, chiorthiamid, grass enemies, etobenzanid (benzchlomet), thiazole Grassy amine, phosphine (fosamin or heptylamine; C4) auxin herbicide, such as picolinic acid, such as ricket grass or gram gram; or 2,4-D or herbicide; C5) auxin Delivery inhibitors, such as naproxil or diflupirone; C6) carotenoid biosynthesis inhibitors, such as piracetone, clomazone/dimethazone, diflufenazone, fluroxypyr, fluridine Oxalone, pyrazol, benzoxazole, isoxaflutole, isoxafluzate, mesotrione, sulcotrione/chlormesulone, ketospiradox, furosemone Grass killing or killing grass; C7) enol acetone oxalic acid-3-phosphate synthase inhibitor (EPSPS), such as glyphosate or phosphinothricin; C8) glutamate synthetase inhibitors, such as Bila Grass (bilanafos /bialaphos) or glufosinate; C9) lipid biosynthesis inhibitors, such as indoline, such as samarium phosphate or mefenacet; chloroacetanilide, such as methotrexate, S-di Methionine, acetochlor, alachlor, butachlor, butachlor, acetochlor, dimethylamine, metazachlor, metolachlor, S-metolachlor , pretilachlor, acetochlor, acetochlor, chlorpyrifos, methoxythiazide or xanthin; thiourea, such as sulbactam, chlorhexidine, oatmeal, piperidan, EPTC, pentane Grass dan, grass chlorpyrifos, gram grass, benzyl herb, thiobencarb/benthiocarb, wild wheat or chlorpyrifos; or furosemide or flufensulfate; C10) mitotic inhibitors, such as amine Formate, such as yellow herb, long-killing grass, chlorpheniramine, pingcao, pronamid/propyzamid, aniline or ketone; dinitroaniline, such as fluramide, dardan, enemy Leamine, butyl fluoride, flufenacil, huangcaoxiao, pendimethalin, alanine or trifluralin; pyridine, such as flurazepam or thiacildine; or oxacillin, diquat (DCPA) or bud dan; C11) protoporphyrinogen IX oxidase inhibitor, such as diphenyl ether, such as acifluorfen, acifluorfen sodium, benzalkonium, bifenox, grass Dry ether (CNP), chlorfenapyr, Ioxacin, flufenacetate, flufenazone, flufenacetate, flufenacil, herbicide ether, nitrofurazone or oxyfluorophene; oxadiazole, such as propargyloxalate or Oxacillin; cyclic quinone imine, such as flufenidine, buffing grass, oxafluridone, ketoxifen, flufenic acid, propifluramide, flumicipine, flupropacil , flurazepam, mesoxalam or thiadiazolamide; or pyrazole, such as ET-751, JV 485 or flufenacet; C12) photosynthesis inhibitors, such as propanil, valerate or pyridine Benzene; benzothiadiazinone, such as bentazone; dinitrophenol, such as chlorfenapyr, dinol, leutroyl ester, tetral or DNOC; bipyridine, such as sedge fast, Benzoin fast, diquat or paraquat; urea, such as chlorbromide, chloromeron, chlorpyrifos, oxazolone, diuron, thiadiazole, chlorpyrifos, humulus, isoproturon , isoproterenol, ligulon, thiazolyl, chlorpyrifos, pyranol, methoxylated, green gluten, chlorhexidine, cyclosporine or decamsung; phenol, such as bromoxynil or iodobenzonitrile Chlorophyll; triazine, such as annihilation , atrazine, cyanazine, desmein, dimethamethryn, hexazinone, chlorpyrifos, chlorpyrifos, chlorpyrifos, simazine, xicajing, methoxy oxalic acid, go Grass net, terbutin or chlorpyrifos; triazinone, such as phenazine Ketone or selezine; uracil, such as herbicidal, cyclohexidine or terpene; or bis-carbamate, such as beet or beetin; C13) synergist, such as ethylene oxide, such as Grass ring; C14) CIS cell wall synthesis inhibitors, such as isoxachlor or dichlorfen; C15) various other herbicides, such as dichloropropionic acid, such as thatch; dihydrobenzofuran, such as ethidium yellow; Phenylacetic acid, such as chlorfenac/fenac; or azide, oatmeal, disperse, benzothiazide, sulfamethoxazole, buminafos, terazosole, acetylacesulfame, oxarib Amine, chlorsulfurin, oatmeal, haylide, cycloheptyl ether, benzalkonium, cyclopyllium, oxadiazepine, tricyclothiamatine, dibenzyluron, herbicide, vanilla, grass Tsu, grass algae, oxazinone, flufensulfuron, flubentranil, fluoxazole, chlorfluralin, isolerin, carbendazim, flufenacetin, chlorpyrifos , chlorfenapyr, diclofenac, chloramphenicol, oxaciclomefone, ketamine, piperacine, cyprodinil, ciprofloxacin, valerian, medusa, G Death (CDEC), roots shoots spirit triazin fluroxypyr amine, triazole 2carboxamide (triazofenamid) or long-trimethoxyphenyl; environmentally compatible salts thereof.

殺線蟲劑或生物殺線蟲劑：免賴得、地蟲威、涕滅碸威、威線肟、二胺磷、芬滅松、硫線磷、除線磷、普伏松、豐索磷、噻唑磷、速殺硫磷、異醯胺松(isamidofof)、依殺松、磷蟲威、硫磷嗪、菸鹼硫磷、甲基減蚜磷、乙醯蟲腈、苯氯噻、氯化苦、邁隆、氟噻蟲碸、1,3-二氯丙烯(二氯丙烯)、二硫二甲烷、威百畝鈉、威百畝鉀、威百畝鹽(所有MITC產生劑)、溴化甲烷、生物土壤改良劑(例如芥菜籽、芥菜籽提取物)、土壤蒸汽薰蒸、異硫氰酸烯丙酯(AITC)、硫酸二甲酯、糠醛(醛)。 Nematicides or bio-nematicides: free of lysine, chlorpyrifos, annihilation, sputum, diamine, fensin, sulphur, phosphorus, pusson, phosphos, Thiazophos, acesulfame, isamimidofof, chlorfenapyr, chlorpyrifos, nicotinic thiophosphorus, methyl phosphazone, acetaminophen, phenylchlorothiophene, chlorination Bitter, Mellon, fluthiazem, 1,3-dichloropropene (dichloropropene), dithiodimethane, Weibaimu sodium, Weibaimu potassium, Weibaimu salt (all MITC generators), bromine Methane, biological soil improver (such as mustard seed, mustard seed extract), soil steam fumigation, allyl isothiocyanate (AITC), dimethyl sulfate, furfural (aldehyde).

本發明之適合植物生長調節因子包括以下各物：植物生長調節因子：D1)抗生長素，諸如降固醇酸、2,3,5-三碘苯甲酸；D2)生長素，諸如4-CPA、2,4-D、2,4-DB、2,4-DEP、2,4-滴丙酸、涕丙酸、IAA、IBA、萘乙醯胺、α-萘乙酸、1-萘酚、萘氧基乙酸、環烷酸 鉀、環烷酸鈉、2,4,5-T；D3)細胞***素，諸如2iP、苄基腺嘌呤、4-羥基苯乙醇、活動素、玉米素；D4)脫葉劑，諸如氰胺化鈣、噻節因、草多索、益收生長素、脫葉亞磷、甲氧隆、五氯苯酚、噻苯隆、脫葉磷；D5)乙烯抑制劑，諸如艾維激素(aviglycine)、1-甲基環丙烯；D6)乙烯釋放劑，諸如ACC、乙烯矽、益收生長素、乙二肟；D7)殺配子劑，諸如噠嗪酮酸鉀、抑芽丹；D8)赤黴素，諸如赤黴素、赤黴酸；D9)生長抑制劑，諸如脫落酸、嘧啶醇、比達寧(butralin)、加保利、三丁氯苄膦、氯苯胺靈、敵草克、氟節胺、增糖胺、殺木膦、草甘二膦、苯嘧丙醇、茉莉酸、抑芽丹、壯棉素、哌壯素、茉莉酮、苯胺靈、調節安(tiaojiean)、2,3,5-三碘苯甲酸；D10)形態素，諸如氯氟酸、氯甲丹、二氯甲丹、抑草丁；D11)生長延緩劑，諸如克美素(chlormequat)、丁醯肼、調嘧醇、氟磺醯草胺、巴克素、四環唑、烯效唑；D12)生長刺激劑，諸如油菜素內酯、油菜素內酯-乙基、DCPTA、氯吡脲、惡黴靈、補骨內酯、三十烷醇；D13)類別不明之植物生長調節劑，諸如菊乙胺酯、氟磺胺草、特克草、香芹酮、氯化膽鹼、苯氰丁醯胺、苯噠嗪酸、氰胺、環丙酸醯胺、環己醯亞胺、環丙磺醯胺、丙醯芸苔素內酯、吲熟酯、乙烯、呋苯硫脲、乙二醇縮糖醛、增產肟、氯乙亞磺酸(holosulf)、依納素、砷酸鉛、滅速克、調環酸、比達農(pydanon)、殺雄啉、抑芽唑、抗倒酯。 Suitable plant growth regulators of the invention include the following: plant growth regulator: D1) anti-auxin, such as treasic acid, 2,3,5-triiodobenzoic acid; D2) auxin, such as 4-CPA , 2,4-D, 2,4-DB, 2,4-DEP, 2,4-D-propionic acid, propionic acid, IAA, IBA, naphthylamine, α-naphthylacetic acid, 1-naphthol, Naphthyloxyacetic acid, naphthenic acid Potassium, sodium naphthenate, 2,4,5-T; D3) cytokinins, such as 2iP, benzyl adenine, 4-hydroxyphenylethanol, activin, zein; D4) defoliants, such as cyanamide Calcium, thiazide, sedum, auxin, defoliated phosphite, methoxylated, pentachlorophenol, thiabendazole, defoliated phosphorus; D5) ethylene inhibitors, such as aviglicine (aviglycine), 1 - methylcyclopropene; D6) ethylene release agent, such as ACC, ethylene oxime, auxin, acetylene; D7) gametocide, such as potassium pyridazinone, buddandan; D8) gibberellin, Such as gibberellic acid, gibberellic acid; D9) growth inhibitors, such as abscisic acid, pyrimidinol, butralin, calprotection, tributyl chlorophosphonazo, chlorpheniramine, diquat, flutica, Glycosaminoglycan, chlorpyrifos, glyphosate, benzipropetate, jasmonic acid, bud dandan, strong cotton pigment, piperazine, jasmonone, aniline, tiaojiean, 2,3,5 -triiodobenzoic acid; D10) morphines, such as chlorofluoric acid, chloromethyl sulphate, dichloromethyl sulphate, sulphate; D11) growth retardants, such as chlormequat, butyl sulfonate, chlorpyrifos, fluoride Sulfachlor Barkerol, tetracyclazole, uniconazole; D12) growth stimulators, such as brassinolide, brassinolide-ethyl, DCPTA, chlorpyrifos, carbendazim, osteol lactone, triacontanol ; D13) Plant growth regulators of unknown class, such as methamine, sulfamethoxazole, teque grass, carvone, choline chloride, benzalkonium amide, benzoxazine acid, cyanamide, cyclopropyl Acid amide, cycloheximide, ciprofloxacin, propican lactone, benzoic acid ester, ethylene, furose thiourea, ethylene glycol acetal, chitosan, chloroethanesulfinic acid (holosulf), enaxin, lead arsenate, chlorhexidine, cyclohexanoic acid, pydanon, chlordamine, oxazole, anti-pivoxil.

肥料可為液體肥料。術語「液體肥料」係指呈含有不同比率之氮、磷及鉀(例如(但不限於)10%氮、34%磷及0%鉀)及微量養分的流體或液體形式之肥料，通常稱為高磷且促進快速及強有力根生長之基肥。 The fertilizer can be a liquid fertilizer. The term "liquid fertilizer" means a fluid or liquid form of fertilizer containing nitrogen, phosphorus and potassium in various ratios (such as, but not limited to, 10% nitrogen, 34% phosphorus and 0% potassium) and micronutrients, commonly referred to as A base fertilizer that is high in phosphorus and promotes rapid and strong root growth.

本發明之化學調配物可呈任何適當習知形式，例如乳液濃縮物(EC)、懸浮液濃縮物(SC)、懸乳乳液(SE)、膠囊懸浮液(CS)、水可分散顆粒(WG)、可乳化顆粒(EG)、油包水乳液(EO)、水包油乳液 (EW)、微乳液(ME)、油性分散液(OD)、油狀可混溶可流動劑(OF)、油狀可混溶液體(OL)、可溶濃縮物(SL)、超低體積懸浮液(SU)、超低體積液體(UL)、可分散濃縮物(DC)、可濕潤粉末(WP)或與農業上可接受之佐劑組合之任何技術上可行調配物。 The chemical formulations of the present invention may be in any suitable conventional form, such as emulsion concentrates (EC), suspension concentrates (SC), suspoemulsion emulsions (SE), capsule suspensions (CS), water dispersible granules (WG). ), emulsifiable particles (EG), water-in-oil emulsion (EO), oil-in-water emulsion (EW), microemulsion (ME), oily dispersion (OD), oily miscible flowable agent (OF), oily miscible solution (OL), soluble concentrate (SL), ultra low volume Suspension (SU), ultra low volume liquid (UL), dispersible concentrate (DC), wettable powder (WP) or any technically feasible formulation in combination with an agriculturally acceptable adjuvant.

在本發明之另一實施例中，提供用組合物塗佈之植物種子，該組合物包括以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有所有其鑑別特徵之突變體的生物純培養物的孢子，以適合於使植物生長獲益及/或對易感病植物賦予保護以避免病原性感染之量存在。植物生長益處及/或所賦予之保護可藉由幼苗活力提高、根發育改善、植物生長改善、植物健康提高、產量增加、外觀改善、對植物病原體之抗性提高、病原性感染減少或其組合展現。 In another embodiment of the present invention, there is provided a plant seed coated with a composition comprising Bacillus amyloliquefaciens RTI472 deposited under ATCC No. PTA-121166 or a mutant thereof having all of its identifying characteristics Spores of pure cultures are present in an amount suitable to benefit plant growth and/or to confer protection to susceptible plants to avoid pathogenic infections. Plant growth benefits and/or protection afforded by increased seedling vigor, improved root development, improved plant growth, improved plant health, increased yield, improved appearance, increased resistance to plant pathogens, reduced pathogenic infection, or combinations thereof Show.

塗佈在植物種子上之組合物可包括每顆種子約1.0×102CFU至每顆種子約1.0×109CFU之量的解澱粉芽孢桿菌孢子。 The composition coated on the plant seed may comprise from about 1.0 x 10 2 CFU per seed to about 1.0 x 109 CFU per seed.

植物種子可包括(但不限於)單子葉植物、雙子葉植物、穀物、玉米、甜玉米、爆玉米、玉米種、青貯玉米、飼料玉米、稻穀、小麥、大麥、高粱、甘藍型蔬菜、椰菜、甘藍菜、花椰菜、芽甘藍、散葉甘蘭、羽衣甘藍、芥菜、球莖甘藍、鱗莖類蔬菜、洋蔥、大蒜、蔥、果蔬菜、胡椒、番茄、茄子、地面櫻桃、樹蕃茄、秋葵、葡萄、香草/香料、瓜類蔬菜、黃瓜、哈密瓜、甜瓜、香甘瓜、番南瓜、西瓜、南瓜、茄子、綠葉蔬菜、萵苣、芹菜、菠菜、香芹、菊苣、豆科植物/蔬菜(多汁且乾燥之豆子及豌豆)、豆子、四季豆、食莢菜豆、殼豆、大豆、乾豆、回回豆、利馬豆、豌豆、鷹嘴豆、豌豆瓣、扁豆、油料種子作物、芥花、蓖麻、棉花、亞麻、花生、油菜籽、紅花、芝麻、向日葵、大豆、根/塊莖及球莖蔬菜、胡蘿蔔、馬鈴薯、甘薯、甜菜、薑、辣根、蘿蔔、人參、蕪菁、甘蔗、甜菜、草或草皮草之種子。 Plant seeds may include, but are not limited to, monocots, dicots, grains, corn, sweet corn, popcorn, corn, silage corn, feed corn, rice, wheat, barley, sorghum, kale, broccoli , kale, broccoli, Brussels sprouts, broccoli, kale, mustard, broccoli, bulbous vegetables, onions, garlic, onions, fruit vegetables, pepper, tomato, eggplant, ground cherry, tree tomatoes, okra, grapes, Vanilla/spice, melon, cucumber, cantaloupe, melon, cantaloupe, squash, watermelon, pumpkin, eggplant, green leafy vegetables, lettuce, celery, spinach, parsley, chicory, legumes/vegetables (succulent and Dried beans and peas), beans, green beans, food pods, shell beans, soybeans, dried beans, peas, lima beans, peas, chickpeas, peas, lentils, oilseed crops, canola, nettle , cotton, flax, peanuts, rapeseed, safflower, sesame, sunflower, soybean, root/tuber and bulbous vegetables, carrots, potatoes, sweet potatoes, beets, ginger, horseradish, radish Seeds of ginseng, turnip, sugar cane, sugar beet, grass or turf grass.

在一或多個實施例中，植物種子可包括乾豆、玉米、小麥、大豆、菜籽、稻穀、黃瓜、胡椒、番茄、番南瓜、棉花、草及草皮草之種子。 In one or more embodiments, the plant seeds can include seeds of dried beans, corn, wheat, soybeans, rapeseed, rice, cucumber, pepper, tomato, squash, cotton, grass, and turfgrass.

藉由經塗佈之植物種子處理之病原性感染可由植物病原體引起，該等植物病原體包括例如(但不限於)植物真菌病原體、植物細菌病原體、葡萄孢屬、灰葡萄孢菌、蔥鱗葡萄孢菌、歐文菌屬、胡蘿蔔軟腐歐文菌、解澱粉歐文菌、黃單孢菌屬、地毯草黃單孢菌、油菜黃單孢菌胡蘿蔔致病變種、桃李黃單孢菌、核桃黃單孢菌、水稻黃單孢菌水稻變種、假單孢菌屬、丁香假單孢菌番茄致病變種、疫黴菌屬、致病疫黴菌、鐮刀菌屬、大刀鐮刀菌、禾穀鐮刀菌、尖孢鐮刀菌、古巴尖孢鐮刀菌、番茄尖孢鐮刀菌、棒形鐮刀菌、寄生疫黴菌、大豆疫黴菌、辣椒疫黴菌、樟疫黴菌、草莓疫黴菌、疫黴菌屬、櫟樹猝死病菌、棕櫚疫黴菌、菸草疫黴菌、絲核菌屬、立枯絲核菌、玉蜀黍絲核菌、稻枯斑絲核菌、褐色絲核菌、禾穀絲核菌、紫紋羽絲核菌、草莓絲核菌、枝生絲核菌、覆盆子絲核菌、豆狀絲核菌、菜豆殼球孢菌、稻瘟菌、腐黴屬、終極腐黴菌、瓜果腐黴菌、畸雌腐黴菌、簇囊腐黴菌、水生腐黴菌、森林腐黴菌、黑粉菌屬、裸黑粉菌、玉蜀黍黑粉菌、甘蔗鞭黑粉菌、麥角菌屬、麥角菌、腥黑粉菌屬、小麥腥黑粉菌、光滑腥黑粉菌、稻粒黑粉菌、小麥矮腥黑粉菌、莖點黴屬、大豆生莖點黴、多變莖點黴、甘藍莖點黴、禾旋孢腔菌、禾頂囊殼、炭疽菌屬或其組合。 Pathogenic infections treated by coated plant seeds may be caused by plant pathogens including, for example, but not limited to, plant fungal pathogens, plant bacterial pathogens, Botrytis, Botrytis cinerea, Botrytis cinerea Bacteria, Owens, Carrot soft rot, Owen bacteria, Xanthomonas, Xanthomonas, Rhizoctonia solani, Xanthomonas camphora, Carotenoids, Rhodopseudomonas, Rhizoctonia solani , Xanthomonas oryzae rice variety, Pseudomonas, Pseudomonas syringae tomato-induced disease, Phytophthora, Phytophthora infestans, Fusarium, Fusarium oxysporum, Fusarium graminearum, Fusarium oxysporum Fusarium, Fusarium oxysporum, Fusarium oxysporum, Fusarium oxysporum, Phytophthora parasitica, Phytophthora sojae, Phytophthora capsici, Phytophthora capsici, Phytophthora strawberry, Phytophthora, eucalyptus, palm plague Mold, Phytophthora nicotiana, Rhizoctonia, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia solani, Strawberry silk nucleus Bacteria Phytophthora, Rhizoctonia solani, Rhizoctonia solani, Coccidioides, Magnaporthe oryzae, Pythium, Pythium, Pythium, Pythium, Pythium, Pythium , forest Pythium, black powder fungus, naked black powder fungus, maize black powder fungus, sugar cane whip black powder fungus, ergot fungus, ergot fungus, black fungus genus, wheat black powder fungus, smooth black Powder fungus, rice black powder fungus, wheat dwarf smut, stalk genus, soybean stalk, mildew, sphaerophyll, sphaerophyll, Helminthosporium, sclerotium, anthrax Genus or a combination thereof.

塗佈至植物種子上之組合物可進一步包括微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑或植物生長調節劑之一或組合，其以適合於使植物生長獲益及/或對易感病植物賦予保護以避免病原性感染之量存在。殺蟲劑可包括畢芬寧。殺線蟲劑可包括硫線磷。殺蟲劑可包括畢芬寧及噻蟲胺。 The composition applied to the plant seed may further comprise one or a combination of a microbial, biological or chemical insecticide, fungicide, nematicide, bactericide or plant growth regulator, which is suitable for benefiting plant growth. And/or the protection of susceptible plants to avoid the presence of pathogenic infections. The insecticide can include diphennin. Nematicides may include sulphur phosphorus. Insecticides may include bifenin and clothianidin.

在本發明之另一實施例中，提供一種用於達成使植物生長獲益或對易感病植物賦予保護以避免病原性感染中之一或兩者的方法，該方法包括：將植物種子種植在適合生長介質中或使植物之營養剪枝/組織在適合生長介質中再生，其中種子已用一種組合物塗佈或營養剪枝/組織業經該組合物接種，該組合物包含以ATCC PTA-121166寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物，其中使植物自種子或營養剪枝/組織之生長獲益及/或賦予保護以避免病原性感染。植物生長益處及/或所賦予之保護可藉由幼苗活力提高、根發育改善、植物生長改善、植物健康提高、產量增加、外觀改善、對植物病原體之抗性提高、病原性感染減少或其組合展現。 In another embodiment of the present invention, there is provided a method for achieving one or both of benefiting plant growth or conferring protection to susceptible plants to avoid pathogenic infections, the method comprising: planting plant seeds The growth pruning/tissue of the plant is in a suitable growth medium or regenerated in a suitable growth medium, wherein the seed has been coated with a composition or the nutrient pruning/tissue is inoculated with the composition comprising ATCC PTA- 121166 A biologically pure culture of Bacillus amyloliquefaciens RTI472 or a mutant thereof having all of its identifying characteristics, wherein plants are benefited from the growth of seed or nutrient pruning/tissue and/or confer protection to avoid pathogenic infection. Plant growth benefits and/or protection afforded by increased seedling vigor, improved root development, improved plant growth, improved plant health, increased yield, improved appearance, increased resistance to plant pathogens, reduced pathogenic infection, or combinations thereof Show.

在一個實施例中，該方法可進一步包括向以下各者施用液體肥料：植物周圍土壤或生長介質；在土壤或生長介質中播種植物種子之前的土壤或生長介質；或在土壤或生長介質中種植植物、植物剪枝、植物移植物或植物癒合組織之前的土壤或生長介質。 In one embodiment, the method may further comprise applying a liquid fertilizer to: soil or growth medium surrounding the plant; soil or growth medium prior to seeding the plant seed in the soil or growth medium; or planting in soil or growth medium Soil or growth medium prior to plant, plant pruning, plant graft or plant healing tissue.

在一個實施例中，提供一種藉由對易感病植物賦予保護以避免或減少病原性感染使植物生長獲益，同時使針對處理之抗性積聚減至最少之方法。該方法包括將第一組合物及第二組合物以分開施用及改變時間間隔輸送至易感病植物，其中第一及第二組合物各者以適合於對植物賦予保護以避免或減少病原性感染之量輸送。第一組合物包括以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物。第二組合物包括一或多種具有殺真菌或殺菌特性之化學活性劑。在該方法中，第一及第二組合物以改變時間間隔輸送至植物葉、植物莖皮、植物果實、植物花、植物種子、植物根部、植物剪枝、植物移植物、植物癒合組織或植物周圍土壤或生長介質之一或組合。在該方法中，賦予保護以避免及/或減少 病原性感染所需之化學活性劑的總量減少且針對處理之抗性積聚減至最少。植物生長益處及/或所賦予之保護可藉由幼苗活力提高、根發育改善、植物生長改善、植物健康提高、產量增加、外觀改善、對植物病原體之抗性提高、病原性感染減少或其組合展現。 In one embodiment, there is provided a method of benefiting plant growth by imparting protection to susceptible plants to avoid or reduce pathogenic infections while minimizing the accumulation of resistance to treatment. The method comprises delivering the first composition and the second composition to a susceptible plant at separate application and varying time intervals, wherein each of the first and second compositions is adapted to confer protection to the plant to avoid or reduce pathogenicity The amount of infection is delivered. The first composition comprises Bacillus amyloliquefaciens RTI472 deposited under ATCC PTA-121166 or a biologically pure culture thereof having mutants of all of its identifying characteristics. The second composition includes one or more chemical active agents having fungicidal or bactericidal properties. In this method, the first and second compositions are delivered to plant leaves, plant stems, plant fruits, plant flowers, plant seeds, plant roots, plant pruning, plant transplants, plant healing tissues or plants at varying time intervals. One or a combination of surrounding soil or growth medium. In this method, protection is given to avoid and/or reduce The total amount of chemically active agent required for pathogenic infection is reduced and the accumulation of resistance to treatment is minimized. Plant growth benefits and/or protection afforded by increased seedling vigor, improved root development, improved plant growth, improved plant health, increased yield, improved appearance, increased resistance to plant pathogens, reduced pathogenic infection, or combinations thereof Show.

第一組合物可進一步包括載劑、界面活性劑、分散劑或酵母提取物之一或組合。酵母提取物可以在約0.01%至0.2%w/w範圍內的使植物生長獲益之施用率輸送。 The first composition may further comprise one or a combination of a carrier, a surfactant, a dispersant, or a yeast extract. The yeast extract can be delivered at an application rate that benefits plant growth in the range of about 0.01% to 0.2% w/w.

在藉由對易感病植物賦予保護以避免或減少病原性感染使植物生長獲益同時將針對處理之抗性積聚減至最少的方法中，改變時間間隔可在相隔1天至10天範圍內且可相隔5至7天。第一次施用之時間安排可視特定作物而定且可在種植時、作物萌芽後幾週、開花時、出現疾病時或在預期疾病出現前的範圍內。第一及第二組合物各者可輸送至植物葉、植物果實或植物花。適合於對植物賦予保護以避免或減少病原性感染的輸送量可為約1.0×1010CFU/ha至約1.0×1014CFU/ha解澱粉芽孢桿菌RTI472及約0.01%至0.2%w/w酵母提取物。 In a method of minimizing the accumulation of resistance to treatment by protecting the susceptible plants by avoiding or reducing pathogenic infections, the time interval can be varied from 1 day to 10 days apart. And can be separated by 5 to 7 days. The timing of the first application may depend on the particular crop and may be within the range of planting, weeks after germination of the crop, flowering, disease, or pre-exposure. Each of the first and second compositions can be delivered to a plant leaf, plant fruit or plant flower. Delivery rates suitable for conferring protection to plants to avoid or reduce pathogenic infections may range from about 1.0 x 10 10 CFU/ha to about 1.0 x 10 14 CFU/ha B. amyloliquefaci RTI 472 and about 0.01% to 0.2% w/w. Yeast extract.

一或多種以分開施用及改變時間間隔輸送至易感病植物之化學活性劑可包括例如(但不限於)以下之一或組合：甲氧基丙烯酸酯(strobilurine)、***、護汰芬(flutriafol)、得克利、丙硫菌唑(prothiaconazole)、氟環唑(expoxyconazole)、氟吡菌醯胺、四氯異苯腈、甲基托布津、氫氧化銅殺真菌劑、基於EDBC之殺真菌劑、鋅錳乃浦(mancozeb)、琥珀酸去氫酶(succinase dehydrogenase，SDHI)殺真菌劑、必殺吩、依普同(iprodione)、達滅芬或威利芬那雷特。 One or more chemically active agents delivered to the susceptible plant at separate administrations and varying time intervals may include, for example, but not limited to, one or a combination of the following: strobilurine, triazole, dimethoate ( Flutriafol), dextran, prothiaconazole, expoxyconazole, fluopyram, tetrachloroisophthalonitrile, methyl thiophanate, copper hydroxide fungicide, EDBC-based fungicide Agent, zinc-manganese (mancozeb), succinase dehydrogenase (SDHI) fungicide, chlorpyrifos, iprodione, dafoxdine or wilifenalide.

一或多種以分開施用及改變時間間隔輸送至易感病植物之化學活性劑可包括氟吡菌醯胺加上得克利且包含RTI472之第一組合物之輸送可替換四氯異苯腈殺真菌劑之輸送。植物可為葫蘆且病原性感染可由白粉病引起。 One or more chemically active agents that are delivered to the susceptible plant at separate administrations and at varying time intervals may include flupirtamine plus dextran and a first composition comprising RTI 472 to deliver a replaceable tetrachloroisocyanuric fungicide Delivery of the agent. The plant can be a gourd and the pathogenic infection can be caused by powdery mildew.

一或多種以分開施用及改變時間間隔輸送至易感病植物之化學活性劑可包括甲基托布津殺真菌劑且包含RTI472之第一組合物之輸送可替換丙硫菌唑殺真菌劑之輸送。 One or more chemically active agents delivered to the susceptible plant in separate application and varying time intervals may comprise a methyl thiophanate fungicide and the delivery of the first composition comprising RTI 472 may be substituted for the delivery of the prothioconazole fungicide .

一或多種以分開施用及改變時間間隔輸送至易感病植物之化學活性劑可包括氫氧化銅殺真菌劑且包含RTI472之第一組合物之輸送可替換四氯異苯腈殺真菌劑之輸送。 One or more chemically active agents delivered to the susceptible plant at separate application and varying time intervals may comprise a copper hydroxide fungicide and the delivery of the replaceable tetrachloroisophthalonitrile fungicide comprising the first composition of RTI 472 .

在一個實施例中，提供一種產品，其包括：包含以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物的第一組分；包含微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑、除草劑、植物提取物、植物生長調節劑或肥料之一或組合的第二組分，其中該第一及第二組分分開包裝且其中各組分為適合於達成使植物生長獲益及/或對易感病植物賦予保護以避免病原性感染中的一或兩者之量；以及用於將適合於使植物生長獲益之量的第一與第二組合物之組合輸送至以下之說明書：植物葉、植物莖皮、植物果實、植物花、植物種子、植物根部、植物剪枝、植物移植物、植物癒合組織；植物周圍土壤或生長介質；在土壤或生長介質中播種植物種子之前的土壤或生長介質；或在土壤或生長介質中種植植物、植物剪枝、植物移植物或植物癒合組織之前的土壤或生長介質。 In one embodiment, a product is provided comprising: a first component comprising a biologically pure culture of Bacillus amyloliquefaciens RTI472 deposited with ATCC PTA-121166 or a mutant having all of its identifying characteristics; a second component of one or a combination of microbial, biological or chemical insecticides, fungicides, nematicides, bactericides, herbicides, plant extracts, plant growth regulators or fertilizers, wherein the first and second The components are packaged separately and wherein the components are suitable for achieving an amount that benefits plant growth and/or confers protection to susceptible plants to avoid one or both of the pathogenic infections; and for The combination of the first and second compositions in the amount of growth benefit is delivered to the following instructions: plant leaves, plant bark, plant fruits, plant flowers, plant seeds, plant roots, plant pruning, plant grafts, plant healing Tissue; soil or growth medium surrounding plants; soil or growth medium prior to planting plant seeds in soil or growth medium; or planting plants, plant pruning in soil or growth medium Graft plant or plant tissue to heal before the soil or growing medium.

在該產品中，殺蟲劑可為擬除蟲菊酯、畢芬寧、七氟菊酯、ξ-賽滅寧、有機磷酸酯、氯氧磷、陶斯松、丁基嘧啶磷、賽扶寧、非潑羅、費普尼、菸鹼類或可尼丁之一或組合。 In this product, the insecticide can be pyrethroid, bifennin, tefluthrin, oxime, chlorpyrifos, organophosphate, oxyphosphonate, taosson, butyl pyrimidine, cyprodin, non-splash One or a combination of Luo, Fipney, nicotinic or cotinine.

在該產品中，第一組合物可進一步包括載劑、界面活性劑、分散劑或酵母提取物之一或組合。 In this product, the first composition may further comprise one or a combination of a carrier, a surfactant, a dispersant or a yeast extract.

在該產品中，第一及第二組合物可呈液體、粉劑、可撒佈顆粒、乾燥可潤濕粉末或乾燥可潤濕顆粒形式。在一個實施例中，第一 組合物呈液體形式，且解澱粉芽孢桿菌RTI472可以約1.0×108CFU/ml至約1.0×1012CFU/ml之濃度存在。在一個實施例中，第一組合物呈粉劑、乾燥可潤濕粉末、可撒佈顆粒或乾燥可潤濕顆粒形式，且解澱粉芽孢桿菌RTI472可以約1.0×108CFU/g至約1.0×1012CFU/g之濃度存在。在一個實施例中，第一組合物呈油性分散液形式且解澱粉芽孢桿菌RTI472以約1.0×108CFU/ml至約1.0×1012CFU/ml之濃度存在。 In this product, the first and second compositions may be in the form of a liquid, a powder, a spreadable granule, a dry wettable powder or a dry wettable granule. In one embodiment, the first composition is in liquid form and the B. amyloliquefaciens RTI 472 can be present at a concentration of from about 1.0 x 10 8 CFU/ml to about 1.0 x 10 12 CFU/ml. In one embodiment, the first composition is in the form of a powder, a dry wettable powder, a spreadable granule or a dry wettable granule, and the B. amyloliquefaciens RTI 472 can be from about 1.0 x 10 8 CFU/g to about 1.0 x. A concentration of 10 12 CFU/g is present. In one embodiment, the first composition is in the form of an oily dispersion and B. amyloliquefaciens RTI 472 is present at a concentration of from about 1.0 x 10 8 CFU/ml to about 1.0 x 10 12 CFU/ml.

在本發明之一個實施例中，提供一種組合物，該組合物包括以下中之至少一者：分離之豐原素-MA化合物、分離之豐原素MB化合物、分離之豐原素MC化合物、分離之去羥基豐原素MA化合物、分離之去羥基豐原素MB化合物、分離之豐原素H化合物、分離之豐原素I化合物及分離之去羥基豐原素I化合物，呈適合於對易感病植物賦予保護以避免病原性感染之量，該化合物具有下式： In one embodiment of the present invention, there is provided a composition comprising at least one of the following: isolated abundance-MA compound, isolated abundance MB compound, isolated abundance MC compound, isolated The hydroxytobarenin MA compound, the isolated hydroxy-hydroxytoxin MB compound, the isolated abundance compound H compound, the isolated abundance compound I compound and the isolated hydroxy-hydroxytoxin I compound are suitable for protecting susceptible plants from The amount of pathogenic infection, the compound has the following formula:

其中R為OH，n在8至20範圍內，FA為直鏈、異或反異，且：對於豐原素MA，X1為Ala，X2為Thr，且X3為Met；對於豐原素MB，X1為Val，X2為Thr，且X3為Met；對於豐原素MC，X1為Aba，X2為Thr，且X3為Met；對於豐原素H，X1為Val，X2為Thr，且X3為Hcy；且對於豐原素I，X1為Ile，X2為Thr，且X3為Ile；以及其中R為H，n在8至20範圍內，FA為直鏈、異或反異，且：對於去羥基豐原素MA，X1為Ala，X2為Thr，且X3為Met；對於去羥基豐原素MB，X1為Val，X2為Thr，且X3為Met；且對於去羥基豐原素I， X1為Ile，X2為Thr，且X3為Ile。 Wherein R is OH, n is in the range of 8 to 20, FA is linear, XOR or di-isomeric, and: for abundance MA, X 1 is Ala, X 2 is Thr, and X 3 is Met; for abundance MB X 1 is Val, X 2 is Thr, and X 3 is Met; for abundance MC, X 1 is Aba, X 2 is Thr, and X 3 is Met; for abundance H, X 1 is Val, X 2 Is Thr and X 3 is Hcy; and for alanogen I, X 1 is Ile, X 2 is Thr, and X 3 is Ile; and wherein R is H, n is in the range of 8 to 20, and FA is linear, XOR or singular, and: for dehydroxylectin MA, X 1 is Ala, X 2 is Thr, and X 3 is Met; for dehydroxyl-rich MB, X 1 is Val, X 2 is Thr, and X 3 Is Met; and for dehydroxylectin I, X 1 is Ile, X 2 is Thr, and X 3 is Ile.

在另一實施例中，該組合物進一步包含表XVI中所列之其他分離之豐原素樣及去羥基豐原素樣化合物之一或組合，呈適合於賦予使植物生長獲益或保護易感病植物以避免病原性感染中的一或兩者之量。 In another embodiment, the composition further comprises one or a combination of other isolated abundance-like and dehydroxy-norformin-like compounds listed in Table XVI, which are suitable for conferring benefits to plants or protecting susceptible diseases. Plants avoid the amount of one or both of the pathogenic infections.

植物生長益處及/或所賦予之保護可藉由幼苗活力提高、根發育改善、植物生長改善、植物健康提高、產量增加、外觀改善、對植物病原體之抗性提高、病原性感染減少或其組合展現。 Plant growth benefits and/or protection afforded by increased seedling vigor, improved root development, improved plant growth, improved plant health, increased yield, improved appearance, increased resistance to plant pathogens, reduced pathogenic infection, or combinations thereof Show.

豐原素-MA、豐原素-MB、豐原素-MC、豐原素-H及豐原素-I化合物及去羥基豐原素-MA、去羥基豐原素-MB及去羥基豐原素-I化合物以及其他豐原素樣及去羥基豐原素樣化合物之一或組合可藉由在熟習此項技術者熟知之適合條件，諸如本文中實例中描述之彼等條件下首先培養RTI472解澱粉芽孢桿菌菌株或產生豐原素-MA、豐原素-MB、豐原素-MC、豐原素-H及豐原素-I化合物及去羥基豐原素-MA、去羥基豐原素-MB及去羥基豐原素-I化合物之另一解澱粉芽孢桿菌菌株，包括(但不限於)在869或M2培養基中培養菌株3至6天來分離。隨後可使用熟習此項技術者熟知之方法進一步分離存在於解澱粉芽抱桿菌培養物上清液中之豐原素樣及去羥基豐原素樣環狀脂肽。舉例而言，解澱粉芽孢桿菌培養物上清液可如本文在實例16所述酸化至pH 2，或用CaCl2(Ajesh,K等人，2013,「Purification and characterization of antifungal lipopeptide from a soil isolated strain of Bacillus cereus.」,Worldwide research efforts in the fighting against microbial pathogens：from basic research to technological developments.A.Mendez-Vilas(編輯).第227-231頁)或NH4SO4(Kim,SH等人，2000,Biotechnol Appl Biochem.31(Pt 3)：249-253)處理，此與或不與有機萃取步驟(Kim,PI等人，2004,J Appl Microbiol.97(5)：942-949)，諸如各種相分離形式，包括(但不限於)直接液體分配、膜超過濾及泡沫分級 分離(Baker,SC等人，2010,Adv Exp Med Biol.672：281-288)組合。 Fengyuansu-MA, Fengyuansu-MB, Fengyuansu-MC, Fengyuansu-H and Fengyuansu-I compounds and hydroxy-hydroxytoxin-MA, hydroxy-hydroxytoxin-MB and hydroxy-hydroxytoxin-I compounds and other Fengyuan One or a combination of a steroid-like and dehydroxy-norformin-like compound can be first cultured by the RTI 472 strain of Bacillus amyloliquefaciens or produced auretolide by suitable conditions well known to those skilled in the art, such as those described in the Examples herein. -MA, amylin-MB, amylin-MC, amyloin-H and amylin-I compounds and a dehydroxyl-prohalin-MA, a dehydroxyl-producin-MB and a dehydroxyl-prohalogen-I compound Bacillus strains, including but not limited to, were cultured in 869 or M2 medium for 3 to 6 days to isolate. The adiponectin-like and hydroxy-hydroxy-proto-like cyclic lipopeptides present in the supernatant of the culture of B. amyloliquefaciens can then be further isolated using methods well known to those skilled in the art. For example, the B. amyloliquefaciens culture supernatant can be acidified to pH 2 as described herein in Example 16, or with CaCl 2 (Ajesh, K et al., 2013, "Purification and characterization of antifungal lipopeptide from a soil isolated Strain of Bacillus cereus.", Worldwide research efforts in the fighting against microbial pathogens: from basic research to technological developments. A. Mendez-Vilas (eds.) pp. 227-231) or NH 4 SO 4 (Kim, SH et al. , 2000, Biotechnol Appl Biochem. 31 (Pt 3): 249-253), with or without organic extraction steps (Kim, PI et al, 2004, J Appl Microbiol. 97(5): 942-949), Combinations such as various phase separation forms include, but are not limited to, direct liquid dispensing, membrane ultrafiltration, and foam fractionation (Baker, SC et al, 2010, Adv Exp Med Biol. 672:281-288).

在一個實施例中，豐原素-MA、豐原素-MB、豐原素-MC、豐原素-H及豐原素-I化合物及去羥基豐原素-MA、去羥基豐原素-MB及去羥基豐原素-I化合物以及表XVII中列出之其他豐原素樣及去羥基豐原素樣化合物之一或組合可自可產生此等化合物之解澱粉芽孢桿菌菌株之生物純培養物分離。 In one embodiment, amylin-MA, amylin-MB, amylin-MC, amylin-H, and a protoxin-I compound and a dehydroxyl-producin-MA, a dehydroxyl-producin-MB, and a dehydroxy-hydroxytoxin The -I compound and one or a combination of other abundance-like and dehydroxy-norformin-like compounds listed in Table XVII can be isolated from biologically pure cultures of B. amyloliquefaciens strains from which such compounds can be produced.

在一個實施例中，豐原素-MA、豐原素-MB、豐原素-MC、豐原素-H及豐原素-I化合物及去羥基豐原素-MA、去羥基豐原素-MB及去羥基豐原素-I化合物以及表XVII中列出之其他豐原素樣及去羥基豐原素樣化合物之一或組合可自以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472之生物純培養物分離。 In one embodiment, amylin-MA, amylin-MB, amylin-MC, amylin-H, and a protoxin-I compound and a dehydroxyl-producin-MA, a dehydroxyl-producin-MB, and a dehydroxy-hydroxytoxin The -I compound and one or a combination of other abundance-like and dehydroxy-norformin-like compounds listed in Table XVII can be isolated from a biologically pure culture of Bacillus amyloliquefaciens RTI472 deposited under ATCC PTA-121166.

在一個實施例中，提供解澱粉芽孢桿菌菌株之生物純培養物的提取物，該提取物包括豐原素-MA、豐原素-MB、豐原素-MC、豐原素-H及豐原素-I化合物及去羥基豐原素-MA、去羥基豐原素-MB及去羥基豐原素-I化合物以及表XVII中所列之其他豐原素樣及去羥基豐原素樣化合物之一或組合。 In one embodiment, there is provided an extract of a biologically pure culture of a B. amyloliquefaciens strain, the extract comprising amylin-MA, amylin-MB, a protosan-MC, a protosan-H, and a protoxin-I compound And one or a combination of dehydroxyhydroxytoxin-MA, dehydroxylungin-MB and dehydroxylectin-I compounds, and other abundance-like and dehydroxy-norformin-like compounds listed in Table XVII.

在一個實施例中，提供以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472之生物純培養物的提取物，該提取物包括豐原素-MA、豐原素-MB、豐原素-MC、豐原素-H及豐原素-I化合物及去羥基豐原素-MA、去羥基豐原素-MB及去羥基豐原素-I化合物及表XVII中列出之其他豐原素樣及去羥基豐原素樣化合物之一或組合。 In one embodiment, an extract of a biologically pure culture of Bacillus amyloliquefaciens RTI472 deposited under ATCC PTA-121166 is provided, the extract comprising amylin-MA, amylin-MB, abundance-MC, Fengyuan素-H and adiponectin-I compounds and hydroxy-hydroxytoxin-MA, hydroxy-hydroxytoxin-MB and hydroxy-hydroxytoxin-I compounds and other abundance-like and dehydroxy-norformin-like compounds listed in Table XVII One or a combination.

在一個實施例中，分子量等於3341.6Da之分離之Ericin S化合物及分子量等於2985.4Da之分離之Ericin A化合物及表XVII中列出之其他豐原素樣及去羥基豐原素樣化合物之一或組合可自可產生此等化合物之解澱粉芽孢桿菌菌株之生物純培養物分離。 In one embodiment, an isolated Ericin S compound having a molecular weight equal to 3341.6 Da and an isolated Ericin A compound having a molecular weight equal to 2985.4 Da and one or a combination of other abundance-like and dehydroxy-norformin-like compounds listed in Table XVII can be used. Bio-pure cultures of Bacillus amyloliquefaciens strains from which such compounds can be produced are isolated.

在一個實施例中，分子量等於3341.6Da之分離之Ericin S化合物 及分子量等於2985.4Da之分離之Ericin A化合物以及表XVII中列出之其他豐原素樣及去羥基豐原素樣化合物之一或組合可自以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472之生物純培養物分離。 In one embodiment, an isolated Ericin S compound having a molecular weight equal to 3341.6 Da And an isolated Ericin A compound having a molecular weight equal to 2985.4 Da and one or a combination of other abundance-like and dehydroxy-hydroxy-like-like compounds listed in Table XVII can be self-administered by ATCC PTA-121166. Biologically pure cultures are isolated.

在一個實施例中，提供解澱粉芽孢桿菌之生物純培養物之提取物，該提取物包括以下中之至少一者：分子量等於3341.6Da之分離之Ericin S化合物或分子量等於2985.4Da之分離之Ericin A化合物。 In one embodiment, an extract of a biologically pure culture of B. amyloliquefaciens is provided, the extract comprising at least one of the following: an isolated Ericin S compound having a molecular weight equal to 3341.6 Da or an isolated Ericin having a molecular weight equal to 2985.4 Da Compound A.

在一個實施例中，提供以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472之生物純培養物的提取物，該提取物包括以下中之至少一者：分子量等於3341.6Da之分離之Ericin S化合物或分子量等於2985.4Da之分離之Ericin A化合物。 In one embodiment, an extract of a biologically pure culture of Bacillus amyloliquefaciens RTI472 deposited under ATCC PTA-121166 is provided, the extract comprising at least one of the following: Ericin S having a molecular weight equal to 3341.6 Da A compound or an isolated Ericin A compound having a molecular weight equal to 2985.4 Da.

在一個實施例中，提供一種組合物，其包含分子量等於3341.6Da之分離之Ericin S化合物及分子量等於2985.4Da之分離之Ericin A化合物中的一或兩者，呈適合於對易感病植物賦予保護以避免病原性感染之量。包括分離之Ericin S化合物及分離之Ericin A化合物中的一或兩者之組合物可進一步包括豐原素-MA、豐原素-MB、豐原素-MC、豐原素-H及豐原素-I化合物及去羥基豐原素-MA、去羥基豐原素-MB及去羥基豐原素-I化合物中之至少一者，且亦視情況包括其他分離之豐原素樣及去羥基豐原素樣化合物之一或組合。 In one embodiment, a composition is provided comprising one or both of an isolated Ericin S compound having a molecular weight equal to 3341.6 Da and an isolated Ericin A compound having a molecular weight equal to 2985.4 Da, which is suitable for imparting a susceptible plant Protect to avoid the amount of pathogenic infections. The composition comprising one or both of the isolated Ericin S compound and the isolated Ericin A compound may further comprise amyromycin-MA, amylin-MB, a protosan-MC, a protosan-H and a protosan-I compound and At least one of a hydroxy amylin-MA, a hydroxy-hydroxytoxin-MB, and a hydroxy-hydroxytoxin-I compound, and optionally, one or a combination of other isolated abundance-like and dehydroxy-norformin-like compounds.

包括Ericin S及Ericin A化合物以及豐原素-MA、豐原素-MB、豐原素-MC、豐原素-H及豐原素-I化合物及去羥基豐原素-MA、去羥基豐原素-MB及去羥基豐原素-I化合物中之至少一者以及視情況其他分離之豐原素樣及去羥基豐原素樣化合物之一或組合的組合物可進一步包括微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑、除草劑、植物提取物、植物生長調節劑或肥料之一或組合，以適合於使植物生長獲益及/或對易感病植物賦予保護以避免病原性感染之量存在。 Including Ericin S and Ericin A compounds as well as agrosperm-MA, abundance-MB, amylin-MC, amylin-H and amylin-I compounds and dehydroxy-hydroxytoxin-MA, dehydroxy-hydroxytoxin-MB and dehydroxyl The composition of at least one of the amylin-I compounds and optionally one or a combination of other isolated abundance-like and dehydroxy-norformin-like compounds may further comprise a microbial, biological or chemical insecticide, fungicide, kill One or a combination of nematodes, bactericides, herbicides, plant extracts, plant growth regulators or fertilizers, suitable for benefiting plant growth and/or conferring protection to susceptible plants to avoid the presence of pathogenic infections .

殺真菌劑可包括來自白羽扇豆之提取物。殺真菌劑可包括BLAD多肽。BLAD多肽可為來自甜羽扇豆(白羽扇豆)之天然存在之種子儲存蛋白的片段，其藉由對真菌細胞壁造成損害且破壞內細胞膜而作用於易感真菌病原體。殺真菌劑可包括約20%BLAD多肽。 The fungicide may include an extract from white lupin. The fungicide can include a BLAD polypeptide. The BLAD polypeptide may be a fragment of a naturally occurring seed storage protein from sweet lupin (white lupin) that acts on a susceptible fungal pathogen by causing damage to the fungal cell wall and disrupting the inner cell membrane. The fungicide may comprise about 20% BLAD polypeptide.

包括Ericin S及Ericin A化合物以及豐原素-MA、豐原素-MB、豐原素-MC、豐原素-H及豐原素-I化合物及去羥基豐原素-MA、去羥基豐原素-MB及去羥基豐原素-I化合物中之至少一者的組合物可呈液體、油性分散液、粉劑、可撒佈顆粒或乾燥可潤濕顆粒形式。 Including Ericin S and Ericin A compounds as well as agrosperm-MA, abundance-MB, amylin-MC, amylin-H and amylin-I compounds and dehydroxy-hydroxytoxin-MA, dehydroxy-hydroxytoxin-MB and dehydroxyl The composition of at least one of the abundance-I compounds can be in the form of a liquid, an oily dispersion, a powder, a dispersible granule or a dry wettable granule.

在一個實施例中，提供一種用於使植物生長獲益及/或賦予保護以避免植物病原性感染之方法，其包括將有效量之包含分離之Ericin S及Ericin A化合物以及豐原素-MA、豐原素-MB、豐原素-MC、豐原素-H及豐原素-I化合物及去羥基豐原素-MA、去羥基豐原素-MB及去羥基豐原素-I化合物以及其他分離之豐原素樣及去羥基豐原素樣化合物之一或組合的提取物或組合物施用於植物或果實或植物根部或根周圍之土壤，以使植物生長獲益及/或賦予保護以避免植物病原性感染。植物生長益處及/或所賦予之保護可藉由幼苗活力提高、根發育改善、植物生長改善、植物健康提高、產量增加、外觀改善、對植物病原體之抗性提高、病原性感染減少或其組合展現。 In one embodiment, a method for benefiting plant growth and/or conferring protection against phytopathogenic infections comprising providing an effective amount of isolated Ericin S and Ericin A compounds and abundance-MA, Fengyuansu-MB, Fengyuansu-MC, Fengyuansu-H and Fengyuansu-I compounds and hydroxy-hydroxytoxin-MA, hydroxy-hydroxytoxin-MB and dehydroxy-hydroxy-proto-I compounds and other isolated abundances and An extract or composition of one or a combination of a dehydroxyl-producin-like compound is applied to the soil of the plant or fruit or plant roots or roots to benefit plant growth and/or to confer protection against phytopathogenic infections. Plant growth benefits and/or protection afforded by increased seedling vigor, improved root development, improved plant growth, improved plant health, increased yield, improved appearance, increased resistance to plant pathogens, reduced pathogenic infection, or combinations thereof Show.

在施用有效量之包含分離之Ericin S及Ericin A化合物及豐原素-MA、豐原素-MB、豐原素-MC、豐原素-H及豐原素-I化合物及去羥基豐原素-MA、去羥基豐原素-MB及去羥基豐原素-I化合物及其他分離之豐原素樣或去羥基豐原素樣化合物之一或組合的提取物或組合物之方法中，植物可包括例如單子葉植物、雙子葉植物、穀物、玉米、甜玉米、爆玉米、種子玉米、青貯玉米、飼料玉米、稻穀、小麥、大麥、高粱、蘆筍、漿果、藍莓、黑莓、樹莓、羅甘莓、越橘、蔓越橘、醋栗、接骨木、黑醋栗、蔓越莓、越莓、甘藍型蔬菜、椰菜、甘 藍菜、花椰菜、芽甘藍、散葉甘藍、羽衣甘藍、芥菜、球莖甘藍、瓜類蔬菜、黃瓜、哈密瓜、甜瓜、香甘瓜、番南瓜、西瓜、南瓜、茄子、鱗莖類蔬菜、洋蔥、大蒜、蔥、柑橘、橙子、葡萄柚、檸檬、紅橘、橘柚、柚子、果蔬菜、胡椒、番茄、地面櫻桃、樹蕃茄、秋葵、葡萄、香草/香料、葉類蔬菜、萵苣、芹菜、菠菜、香芹、菊苣、豆科植物/蔬菜(多汁且乾燥之豆子及豌豆)、豆子、四季豆、食莢菜豆、殼豆、大豆、乾豆、回回豆、利馬豆、豌豆、鷹嘴豆、豌豆瓣、扁豆、油料種子作物、芥花、蓖麻、椰子、棉花、亞麻、油棕櫚、橄欖、花生、油菜籽、紅花、芝麻、向日葵、大豆、梨果、蘋果、海棠果、梨、榅桲、夏花山楂、根/塊莖及球莖蔬菜、胡蘿蔔、馬鈴薯、甘薯、木薯、甜菜、薑、辣根、蘿蔔、人參、蕪菁、核果、杏子、櫻桃、油桃、桃子、李子、梅子、草莓、堅果、杏仁、開心果、山核桃、核桃、榛子、板栗、腰果、山毛櫸堅果、胡桃、夏威夷果、獼猴桃、香蕉、(藍)龍舌蘭、草、草皮草、觀賞植物、一品紅、硬木剪枝、栗樹、橡樹、楓樹、甘蔗或甜菜。 In the application of an effective amount of isolated Ericin S and Ericin A compounds and amylin-MA, abundance-MB, abundance-MC, amylin-H and amylin-I compounds and dehydroxyl-proto-ana-MA, dehydroxyl In the method of extracting or extracting a prostaglandin-MB and a dehydroxyl-precipitin-I compound and one or a combination of one of the isolated abundance or dehydroxy-norformin-like compounds, the plant may comprise, for example, a monocotyledon, a dicotyledonous Plants, grains, corn, sweet corn, popcorn, seed corn, silage corn, feed corn, rice, wheat, barley, sorghum, asparagus, berries, blueberries, blackberries, raspberries, raspberries, cranberries, cranberries , gooseberry, elderberry, black currant, cranberry, berry, cabbage, broccoli, gan Blue cabbage, broccoli, Brussels sprouts, loose cabbage, kale, mustard green, stalk cabbage, melon vegetables, cucumber, cantaloupe, melon, sweet melon, pumpkin, watermelon, pumpkin, eggplant, bulbous vegetables, onions, garlic , onion, citrus, orange, grapefruit, lemon, red orange, tangerine, grapefruit, fruit and vegetable, pepper, tomato, ground cherry, tree tomato, okra, grape, vanilla/spice, leafy vegetables, lettuce, celery, Spinach, parsley, chicory, legumes/vegetables (succulent and dry beans and peas), beans, green beans, pod beans, shell beans, soybeans, dried beans, peas, lima beans, peas, garbanzo Beans, peas, lentils, oilseed crops, canola, ramie, coconut, cotton, flax, oil palm, olive, peanut, rapeseed, safflower, sesame, sunflower, soybean, pear, apple, jellyfish, pear , alfalfa, summer flower hawthorn, roots/tubers and bulbous vegetables, carrots, potatoes, sweet potatoes, cassava, beets, ginger, horseradish, radish, ginseng, turnip, stone fruit, apricots, cherries, nectarines, peaches Plum, plum, strawberry, nut, almond, pistachio, pecan, walnut, hazelnut, chestnut, cashew, beech nut, walnut, macadamia, kiwi, banana, (blue) agave, grass, turf, ornamental Poinsettia, hardwood pruning, chestnut, oak, maple, sugar cane or beet.

在施用有效量之包含分離之Ericin S及Ericin A化合物及豐原素-MA、豐原素-MB、豐原素-MC、豐原素-H及豐原素-I化合物及去羥基豐原素-MA、去羥基豐原素-MB及去羥基豐原素-I化合物及其他分離之豐原素樣或去羥基豐原素樣化合物之一或組合的提取物或組合物之方法中，病原性感染可由植物病原體引起，該等植物病原體包括例如植物真菌病原體、植物細菌病原體、鏽菌、葡萄孢屬、灰葡萄孢菌、蔥鱗葡萄孢菌、歐文菌屬、胡蘿蔔軟腐歐文菌、解澱粉歐文菌、狄克氏菌屬、達旦狄克氏菌、黑脛病菌、土壤桿菌屬、根癌土壤桿菌、黃單孢菌屬、地毯草黃單孢菌、油菜黃單孢菌胡蘿蔔致病變種、桃李黃單孢菌、核桃黃單孢菌、水稻黃單孢菌水稻變種、焦枯菌屬、葉緣焦枯菌、卡地菌屬、韌皮部桿菌、鐮刀菌屬、大刀鐮刀菌、禾穀鐮刀 菌、尖孢鐮刀菌、古巴尖孢鐮刀菌、番茄尖孢鐮刀菌、棒形鐮刀菌、核盤菌屬、向日葵核盤菌、小核盤菌、同果核盤菌、尾孢菌屬、鉤絲殼屬、葡萄白粉病菌(白粉病)、叉絲單囊殼屬(白粉病)、白叉絲單囊殼、克蘭德叉絲單囊殼、擬莖點菌屬、葡萄擬莖點黴病菌、交鏈孢菌屬、細極鏈格孢菌、蔥鏈格孢菌、交鏈格孢菌、立枯交鏈孢菌、鏈格孢菌、假單胞菌屬、丁香假單胞菌番茄變種、疫黴菌屬、致病疫黴菌、寄生疫黴菌、大豆疫黴菌、辣椒疫黴菌、樟疫黴菌、草莓疫黴菌、疫黴菌屬、櫟樹猝死病菌、棕櫚疫黴菌、菸草疫黴菌、層鏽菌屬、豆薯層鏽菌、山馬蝗層鏽菌、曲黴菌屬、黃麴黴、黑麯黴、單孢鏽菌屬、菜豆銹病菌、枝孢菌屬、多主枝孢黴、根黴菌屬、少根根黴菌、青黴菌屬、絲核菌屬、立枯絲核菌、玉蜀黍絲核菌、稻枯斑絲核菌、褐色絲核菌、禾穀絲核菌、紫紋羽絲核菌、草莓絲核菌、枝生絲核菌、覆盆子絲核菌、豆狀絲核菌、菜豆殼球孢菌、稻瘟菌、球腔菌屬、禾生球腔菌、斐濟球腔菌(黑色香蕉葉斑病)、蘋果斑點球腔菌、檸檬球腔菌、稻瘟菌屬、稻瘟病菌、鏈核盤菌屬、桃褐腐病菌、藍莓僵果病病原菌、核果鏈核盤菌、炭疽菌屬、膠孢炭疽菌、辣椒炭疽菌、石斛炭疽菌、間座殼屬、柑桔間座殼菌、棒孢菌屬、多主棒孢菌、膠鏽菌屬、植物受檜膠鏽菌、裂盾菌屬、仁果裂盾菌、黏殼孢菌屬、梨果黏殼孢菌、葡萄座腔菌屬、葡萄潰瘍病病菌、明孢盤菌屬、威爾遜孢殼菌屬、嗜果威爾遜孢殼菌、單囊殼菌屬、斑點單囊殼菌、薔薇單囊殼菌、白粉菌屬、殼多孢菌屬、穎枯殼針孢、腐黴屬、終極腐黴菌、瓜果腐黴菌、畸雌腐黴菌、簇囊腐黴菌、水生腐黴菌、森林腐黴菌、黑星菌屬、蘋果黑星病菌、輪枝孢菌屬、黑粉菌屬、裸黑粉菌、玉蜀黍黑粉菌、甘蔗鞭黑粉菌、麥角菌屬、麥角菌、腥黑粉菌屬、小麥腥黑粉菌、光滑腥黑粉菌、稻粒黑粉菌、小麥矮腥黑粉菌、莖點黴屬、大豆生莖點黴、多變莖點黴、甘藍莖點黴、禾旋孢腔菌、 禾頂囊殼、炭疽菌屬、雲紋病菌屬、大麥雲紋病菌、離蠕孢屬、長蠕孢屬、大麥長蠕孢、玉蜀黍長蠕孢、索萊長蠕孢及小麥褐斑長蠕孢或其組合。 In the application of an effective amount of isolated Ericin S and Ericin A compounds and amylin-MA, abundance-MB, abundance-MC, amylin-H and amylin-I compounds and dehydroxyl-proto-ana-MA, dehydroxyl In a method of extracting or extracting an abundance of the abundance of the abundance of the abundance of the abundance of the abundance of the abundance of the abundance of the abundance of the abundance of the alkaloids Plant pathogens include, for example, plant fungal pathogens, plant bacterial pathogens, rust fungi, Botrytis, Botrytis cinerea, Botrytis cinerea, Owen genus, Carrot soft rot, Erwinia, Diracium, D. dandelion, black smut, Agrobacterium, Agrobacterium tumefaciens, Xanthomonas, Rhizoctonia solani, Xanthomonas campestris, carcinogenic species, P. xylophilus, Walnut yellow Monospora, rice xanthomonas oryzae, rice, genus, genus, bacterium, genus phloem, genus Fusarium, Fusarium oxysporum Fusarium, Fusarium oxysporum, Fusarium oxysporum, Fusarium oxysporum, Fusarium solani, Sclerotinia, S. sclerotiorum, Sclerotinia sclerotiorum, Sclerotinia sclerotiorum, Cercospora, Helicobacter, grape powdery mildew (powder disease), stellate sclerotium (white powder), white stalk single-shell, kelund fork single-shell, Pseudomonas, grape stem point Mold fungus, Alternaria, Alternaria alternata, Alternaria alternata, Alternaria alternata, Alternaria alternata, Alternaria, Pseudomonas, clove pseudomonad Tomato strain, Phytophthora, Phytophthora infestans, Phytophthora infestans, Phytophthora sojae, Phytophthora capsici, Phytophthora capsici, Phytophthora infestans, Phytophthora, eucalyptus, Phytophthora palm, Phytophthora nicotiana, Pseudomonas, Pleurotus ostreatus, P. sylvestris, Aspergillus, Aspergillus, Aspergillus niger, Puccinia, Bean rust, Cladosporium, C. Rhizopus, Rhizopus, Penicillium, Rhizoctonia, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia solani Rhizoctonia cerealis, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia solani, Coccidioides, Magnaporthe oryzae , G. sphaeroides, Fischer's bacterium (black banana leaf spot), apple spotted bacterium, lemon bacterium, rice blast, rice blast, chain genus, brown rot, blueberry Zygootic pathogens, Sclerotinia sclerotiorum, Anthrax, Agaricus bisporus, Capsicum anthracis, Anthracnose, Aphis citrifolia, Citrus occidentum, Corynebacterium, Pseudomonas aeruginosa , rust fungus, plant rust rust fungus, sclerotium genus, lentil licheniformis, genus genus, genus Phaeocystis, genus genus, grape canker, genus Genus, genus, genus, genus, genus, genus, genus, genus, genus, genus, genus, genus, genus, genus, genus, genus , Pythium, Pythium, Pythium, Pythium, Pythium, Pythium, Pythium, Pseudostellaria, Apple Black Phytophthora, Verticillium, sphaerotheca, naked black powder fungus, black ash fungus, sugarcane whitish, ergot, ergot, smut, wheat smut, Smooth black powder fungus, rice black powder fungus, wheat dwarf black powder fungus, stem mold, soybean shoot stem mold, polymorphic stem mold, cabbage stem mold, Helminthosporium, Hessian shell, anthracnose, genus, genus, barley, genus, Helminthosporium, Helminthosporium, Helminthosporium, Helminthosporium, Helminthosporium and Wheat brown spot Spore or a combination thereof.

實例Instance

包括以下實例以為一般技術者實施本發明揭示之標的物之代表性實施例提供指導。鑒於本發明及此項技術中之一般技術水準，一般技術者可瞭解，以下實例意欲僅為例示性的且可在不背離當前所揭示標的物之範疇的情況下採用許多變化、修改及改變。 The following examples are included to provide guidance for the general practitioner to implement representative embodiments of the subject matter disclosed herein. In view of the present invention and the general technical level of the present invention, it is to be understood by those skilled in the art that the following examples are intended to be illustrative only and that many variations, modifications and changes may be employed without departing from the scope of the presently disclosed subject matter.

實例1Example 1 經由序列分析鑑別細菌分離株為解澱粉芽孢桿菌Bacillus amyloliquefaciens

將本文中稱為RTI472之植物相關菌株自生長在北卡羅來納州之西洋參根部分離。將RTI472菌株之16S rRNA及rpoB基因定序，且隨後使用BLAST與NCBI及RDP資料庫中之其他已知細菌菌株進行比較。確定RTI472(SEQ ID NO：1)之16S RNA部分序列與解澱粉芽孢桿菌菌株NS6(KF177175)、解澱粉芽孢桿菌菌株FZB42(NR_075005)及枯草芽孢桿菌亞種枯草菌株DSM 10(NR_027552)之16S rRNA基因序列一致。另外，確定RTI472之rpoB序列與已知之解澱粉芽孢桿菌AS43.3菌株具有最高程度之序列相似性(亦即99%序列一致性)；然而，在DNA層面上存在10個核苷酸差異，表明RTI472為解澱粉芽孢桿菌之新菌株。RTI472菌株經鑑別為解澱粉芽孢桿菌。在DNA層面上rpoB基因之序列差異表明RTI472為解澱粉芽孢桿菌之新菌株。解澱粉芽孢桿菌RTI472於2014年4月17日依據國際承認用於專利程序的微生物寄存布達佩斯條約之條款寄存於美國菌種保藏中心(ATCC)(Manassas,Virginia,USA)且具有專利寄存編號PTA-121166。 A plant-associated strain referred to herein as RTI 472 is isolated from the roots of American ginseng grown in North Carolina. The 16S rRNA and rpoB genes of the RTI472 strain were sequenced and subsequently compared to other known bacterial strains in the NCBI and RDP databases using BLAST. To determine the 16S RNA partial sequence of RTI472 (SEQ ID NO: 1) and the 16S rRNA of B. amyloliquefaciens strain NS6 (KF177175), B. amyloliquefaciens strain FZB42 (NR_075005) and Bacillus subtilis subsp. subtilis strain DSM 10 (NR_027552) The gene sequence is identical. In addition, it was determined that the rpoB sequence of RTI472 has the highest degree of sequence similarity (ie, 99% sequence identity) to the known strain of B. amyloliquefaciens AS43.3; however, there are 10 nucleotide differences at the DNA level, indicating RTI472 is a new strain of Bacillus amyloliquefaciens. The RTI472 strain was identified as B. amyloliquefaciens. The sequence difference of the rpoB gene at the DNA level indicates that RTI472 is a new strain of B. amyloliquefaciens. Bacillus amyloliquefaciens RTI472 was deposited with the American Type Culture Collection (ATCC) (Manassas, Virginia, USA) on April 17, 2014 under the terms of the Budapest Treaty on the International Recognition of Microorganisms for the Purposes of Patent Procedures and has the patent registration number PTA- 121166.

實例2Example 2 解澱粉芽孢桿菌RTI472菌株中與羊毛硫抗生素生物合成相關之獨特Uniqueness associated with lantibiotic biosynthesis in Bacillus amyloliquefaciens RTI472 strain 基因gene

解澱粉芽孢桿菌RTI472菌株之基因組的進一步序列分析揭露此菌株具有同源物在其他緊密相關解澱粉芽孢桿菌菌株中缺乏的與羊毛硫抗生素生物合成相關之獨特基因。羊毛硫肽生物合成簇在解澱粉芽孢桿菌RTI472中經由使用RAST、antiSMASH及BAGEL生物信息學平台進行計算分析來鑑別。RAST基因組註釋鑑別具有蛋白質序列假定與羊毛硫肽生物合成有關之基因的區域。此藉由antiSMASH資料證實，其鑑別相同基因組區域為含有羊毛硫肽生物合成簇。圖1展示與2種緊密相關之解澱粉芽孢桿菌菌株相比，在解澱粉芽孢桿菌RTI472中發現之羊毛硫抗生素生物合成簇之基因組結構的示意圖。圖1中，頂部箭頭組表示RTI472菌株之蛋白質編碼區，其中指示相對轉錄方向。為進行比較，兩種緊密相關之解澱粉芽孢桿菌參考菌株FZB42(中間)及TrigoCor1448(底部)的對應區域展示在RTI472菌株下面。與兩種參考菌株相比由RTI472菌株之基因編碼的蛋白質之胺基酸一致性程度由代表性箭頭之陰影程度以及箭頭內指示之一致性百分比兩者指示。自圖1可觀測到FZB42及TrigoCor1448菌株缺乏許多存在於此簇中之基因，且存在之許多基因內序列一致性程度低。 Further sequence analysis of the genome of Bacillus amyloliquefaciens RTI 472 strain revealed that this strain has a unique gene associated with lantibiotic biosynthesis that is homologous in other closely related B. amyloliquefaciens strains. The lanthion biosynthesis cluster was identified in B. amyloliquefaciens RTI472 by computational analysis using the RAST, antiSMASH and BAGEL bioinformatics platforms. The RAST genome annotation identifies regions with genes whose protein sequences are assumed to be involved in the biosynthesis of lanthiopeptide. This was confirmed by antiSMASH data, which identified the same genomic region as containing a thiol peptide biosynthetic cluster. Figure 1 shows a schematic representation of the genomic structure of a lantibiotic biosynthetic cluster found in B. amyloliquefaciens RTI472 compared to two closely related strains of B. amyloliquefaciens. In Figure 1, the top arrow group indicates the protein coding region of the RTI 472 strain, indicating relative transcriptional orientation. For comparison, the corresponding regions of the two closely related B. amyloliquefaciens reference strains FZB42 (middle) and TrigoCor 1448 (bottom) are shown below the RTI472 strain. The degree of amino acid identity of the protein encoded by the gene of the RTI 472 strain compared to the two reference strains is indicated by both the degree of shading of the representative arrows and the percent identity indicated within the arrows. It can be observed from Figure 1 that the FZB42 and TrigoCor1448 strains lack many of the genes present in this cluster, and many of the genes present have a low degree of sequence identity.

RAST資料之初始檢查鑑別出RTI472菌株基因屬於I類羊毛硫肽合成路徑，除前肽(簇之「A」基因)外其似乎為完整的。關於RTI472菌株之RAST資料的進一步檢查揭露兩個基因(推定生物合成基因與推定免疫性基因)之間不具有任何相關假設基因的區域(大約400bp)。隨後AntiSMASH用於鑑別此區域內推定核心肽序列。為證實此結果，使用方案BAGEL，分析此區域之核苷酸序列，該方案BAGEL預測鑑別為Ericin S前肽之核心肽。雖然基於生物信息學分析解澱粉芽孢桿菌RTI472似乎缺乏Ericin A之前肽，但隨後HPLC/MS/MS資料揭露RTI472菌株之培養物上清液中存在Ericin A(MW 2985.4Da)及Ericin S (MW 3341.6Da)兩者。圖2A中展示解澱粉芽孢桿菌RTI472菌株及稱為「FB005」之未公開之解澱粉芽孢桿菌菌株的代謝物型態之比較。圖2A中之圖展示RTI472菌株產生Ericin A及Ericin S肽，而對於FB005菌株，未偵測到Ericin A或Ericin S。 The initial examination of the RAST data identified the RTI472 gene as belonging to the class I lutein synthesis pathway, which appeared to be complete except for the propeptide (the "A" gene of the cluster). Further examination of the RAST data for the RTI 472 strain revealed a region (approximately 400 bp) that does not have any relevant putative genes between the two genes (a putative biosynthetic gene and a putative immune gene). AntiSMASH was then used to identify putative core peptide sequences in this region. To confirm this result, the nucleotide sequence of this region was analyzed using the protocol BAGEL, which was identified as the core peptide of the Ericin S propeptide. Although B. amyloliquefaci RTI472 appears to lack the Ericin A pre-peptide based on bioinformatics analysis, subsequent HPLC/MS/MS data revealed the presence of Ericin A (MW 2985.4 Da) and Ericin S in the culture supernatant of the RTI 472 strain. (MW 3341.6Da) Both. A comparison of the metabolite profiles of the B. amyloliquefaciens RTI 472 strain and the undisclosed B. amyloliquefaciens strain designated "FB005" is shown in Figure 2A. The graph in Figure 2A shows that the RTI 472 strain produces Ericin A and Ericin S peptides, while for the FB005 strain, Ericin A or Ericin S was not detected.

隨後RTI472與可獲得之解澱粉芽孢桿菌基因組之間的基因組-基因組比較展示參考基因組中無一者具有功能性Ericin S生物合成簇。然而，許多基因組對此基因簇之抗性部分具有低同源性命中。圖2B-2C為展示在RTI472菌株及7種參考解澱粉芽孢桿菌基因組中鑑別之羊毛硫肽生物合成簇之間的比較之表。表展示7種參考解澱粉芽孢桿菌基因組之區域中同線性喪失。7種參考菌株中無一者具有功能性Ericin S生物合成簇。另外，基於文獻之搜索推斷枯草芽孢桿菌A1/3為先前報導具有產生Ericin S之生物合成簇之唯一其他菌株(參見Stein等人，2002,J.Bacteriol.第184卷，第6期，1703)。因此，新鑑別之RTI472菌株具有產生MW=3341.6Da之Ericin S分子及MW為2985.4Da之Ericin A分子的羊毛硫肽合成路徑，此為解澱粉芽孢桿菌菌株獨特的。 A genomic-genomic comparison between RTI 472 and the available B. amyloliquefaciens genome then shows that none of the reference genomes has a functional Ericin S biosynthetic cluster. However, many genomes have low homology hits on the resistant portion of this gene cluster. 2B-2C are tables showing a comparison between the thiothiazide biosynthesis clusters identified in the RTI 472 strain and the seven reference B. amyloliquefaciens genomes. The table shows the same linear loss in the region of the seven reference B. amyloliquefaciens genomes. None of the seven reference strains had a functional Ericin S biosynthetic cluster. In addition, a literature-based search inferred B. subtilis A1/3 as the only other strain previously reported to have a biosynthetic cluster producing Ericin S (see Stein et al., 2002, J. Bacteriol. Vol. 184, No. 6, 1703). . Therefore, the newly identified RTI472 strain has a thiol peptide synthesis pathway which produces an Ericin S molecule of MW = 3341.6 Da and an Ericin A molecule of 2985.4 Da, which is unique to the B. amyloliquefaciens strain.

實例3Example 3 解澱粉芽孢桿菌RTI472分離株之抗微生物特性Antimicrobial properties of Bacillus amyloliquefaciens RTI472 isolates

在盤分析中量測分離株對抗主要植物病原體之拮抗能力。藉由在869瓊脂培養盤上以4cm距離使細菌分離株及病原性真菌並排生長進行評估對抗植物真菌病原體之拮抗作用的盤分析。在室溫下培育培養盤，且長達兩週定期檢查生長行為，諸如生長抑制、地位佔用或無效果。在針對對抗細菌病原體之拮抗特性篩選之情況下，病原體首先在869瓊脂培養盤上擴散為菌苔。隨後，將RTI472培養物之20μl等分試樣點樣於培養盤上。在室溫下培育培養盤，且長達兩週定期檢查已施用RTI472之位置周圍的菌苔中之抑制區。下表I展示拮抗活性之概述。 The antagonistic ability of the isolate against major plant pathogens was measured in disk assays. Disc analysis against antagonism of plant fungal pathogens was evaluated by growing bacterial isolates and pathogenic fungi side by side on a 869 agar plate at 4 cm distance. The plates were incubated at room temperature and examined for growth behavior for up to two weeks, such as growth inhibition, status occupancy or no effect. In the case of screening for antagonistic properties against bacterial pathogens, the pathogen first spreads to the lawn on the 869 agar plate. Subsequently, a 20 μl aliquot of the RTI 472 culture was spotted onto the plate. The plate was incubated at room temperature and the zone of inhibition in the lawn around the site where RTI 472 had been applied was periodically checked for up to two weeks. Table I below shows an overview of antagonistic activity.

實例4Example 4 解澱粉芽孢桿菌RTI472分離株之表現性狀Performance traits of Bacillus amyloliquefaciens RTI472 isolates

除拮抗特性之外，亦量測解澱粉芽孢桿菌472菌株之各種表現性狀，且資料展示於下表II中。根據表II下方文字中所描述之程序進行分析。 In addition to the antagonistic properties, various performance traits of the Bacillus amyloliquefaciens 472 strain were also measured and the data are shown in Table II below. Analyze according to the procedure described in the text below Table II.

表II. 表現型分析：解澱粉芽孢桿菌RTI472分離株之植物激素產生、乙偶姻及吲哚乙酸(IAA)及養分循環 Table II. Phenotypic analysis: Phytohormone production, acetoin and indole acetic acid (IAA) and nutrient cycling in Bacillus amyloliquefaciens RTI472 isolates

酸及乙偶姻測試. 將20μl含菌元培養物之869培養基轉移至1ml甲基紅-Voges Proskauer培養基(Sigma Aldrich 39484)。培養物在30℃、200rpm下培育2天。轉移0.5ml培養物，且添加50μl 0.2g/l甲基紅。紅色指示產酸。其餘0.5ml培養物與0.3ml 5%α-萘酚(Sigma Aldrich N1000)，隨後0.1ml 40%KOH混合。培育30分鐘後解譯樣品。紅色顯影指示乙偶姻產生。對於酸及乙偶姻測試，未接種培養基用作陰性對照(Sokol等人，1979,Journal of Clinical Microbiology.9：538-540)。 Acid and acetoin test. 20 μl of 869 medium containing bacterial culture was transferred to 1 ml of methyl red-Voges Proskauer medium (Sigma Aldrich 39484). The culture was incubated at 30 ° C, 200 rpm for 2 days. 0.5 ml of the culture was transferred, and 50 μl of 0.2 g/l methyl red was added. Red indicates acid production. The remaining 0.5 ml of the culture was mixed with 0.3 ml of 5% alpha-naphthol (Sigma Aldrich N1000) followed by 0.1 ml of 40% KOH. Samples were interpreted after 30 minutes of incubation. Red development indicates the occurrence of acetoin. For the acid and acetoin tests, uninoculated medium was used as a negative control (Sokol et al., 1979, Journal of Clinical Microbiology. 9: 538-540).

吲哚-3-乙酸. 將20μl含菌元培養物之豐富869培養基轉移至補充有0.5g/l色胺酸之1ml 1/10 869培養基(Sigma Aldrich T0254)。在暗處在30℃、200RPM下培育培養物4-5天。使樣品離心，且使0.1ml上清液與0.2ml薩爾科夫斯基氏試劑(Salkowski's Reagent)(35%過氯酸，10mM FeCl3)混合。在暗處培育30分鐘後，產生粉紅色之樣品記錄為對IAA合成呈陽性。IAA稀釋液(Sigma Aldrich I5148)用作陽性比較；未接種培養基用作陰性對照(Taghavi等人，2009,Applied and Environmental Microbiology 75：748-757)。 Indole-3-acetic acid. 20 μl of the rich 869 medium containing the bacterial culture was transferred to 1 ml of 1/10 869 medium (Sigma Aldrich T0254) supplemented with 0.5 g/l of tryptophan. Cultures were incubated for 4-5 days at 30 ° C, 200 RPM in the dark. The sample was centrifuged and the supernatant was mixed with 0.2ml 0.1ml so Sal Khodorkovsky's reagent (Salkowski's Reagent) (35% perchloric acid, 10mM FeCl 3). After incubation for 30 minutes in the dark, the pink-producing sample was recorded as positive for IAA synthesis. IAA dilution (Sigma Aldrich I5148) was used as a positive comparison; uninoculated medium was used as a negative control (Taghavi et al, 2009, Applied and Environmental Microbiology 75: 748-757).

磷酸鹽溶解測試. 將細菌接種於皮科夫斯卡婭(PVK)瓊脂培養基上，該培養基由每公升10g葡萄糖、5g三磷酸鈣、0.2g氯化鉀、0.5g硫酸銨、0.2g氯化鈉、0.1g硫酸鎂七水合物、0.5g酵母提取物、2mg硫酸錳、2mg硫酸鐵及15g瓊脂組成，pH為7，高壓滅菌。清除區指示磷酸鹽溶解細菌(Sharma等人，2011,Journal of Microbiology and Biotechnology Research 1：90-95)。 Phosphate dissolution test. Bacteria were inoculated on Picokovskaya (PVK) agar medium containing 10 g of glucose per liter, 5 g of calcium triphosphate, 0.2 g of potassium chloride, 0.5 g of ammonium sulfate, 0.2 g of chlorination. Sodium, 0.1 g of magnesium sulfate heptahydrate, 0.5 g of yeast extract, 2 mg of manganese sulfate, 2 mg of iron sulfate and 15 g of agar, pH 7, and autoclaved. The clearance zone indicates phosphate lytic bacteria (Sharma et al, 2011, Journal of Microbiology and Biotechnology Research 1: 90-95).

殼質酶活性. 將10%濕重膠狀殼質添加至經改質之PVK瓊脂培養基(每公升10g葡萄糖、0.2g氯化鉀、0.5g硫酸銨、0.2g氯化鈉、0.1g硫酸鎂七水合物、0.5g酵母提取物、2mg硫酸錳、2mg硫酸鐵及15g瓊脂，pH為7，高壓滅菌)中。細菌塗鋪在此等殼質盤上；清除區指示殼質酶活性(N.K.S.Murthy及Bleakley.,2012.「Simplified Method of Preparing Colloidal Chitin Used for Screening of Chitinase Producing Microorganisms」.The Internet Journal of Microbiology.10(2))。 Chitinase activity. Add 10% wet weight gelatinous shell to the modified PVK agar medium (10g glucose, 0.2g potassium chloride, 0.5g ammonium sulfate, 0.2g sodium chloride, 0.1g magnesium sulfate per liter) Heptahydrate, 0.5 g yeast extract, 2 mg manganese sulfate, 2 mg iron sulfate and 15 g agar, pH 7, autoclaved). Bacteria are plated on these hull disks; the clearance zone indicates chitinase activity (NKSMurthy and Bleakley., 2012. "Simplified Method of Preparing Colloidal Chitin Used for Screening of Chitinase Producing Microorganisms". The Internet Journal of Microbiology. 10 ( 2)).

蛋白酶活性. 細菌塗鋪在補充有10%牛奶之869瓊脂培養基上。清除區指示分解蛋白質之能力，表明蛋白酶活性(Sokol等人，1979,Journal of Clinical Microbiology.9：538-540)。 Protease activity. Bacteria were spread on 869 agar medium supplemented with 10% milk. The clearance zone indicates the ability to decompose proteins, indicating protease activity (Sokol et al., 1979, Journal of Clinical Microbiology. 9: 538-540).

實例5Example 5 菜豆鏽病菌及灰葡萄孢菌之解澱粉芽孢桿菌RTI472拮抗作用Antagonistic effect of Bacillus amyloliquefaciens RTI472 and Botrytis cinerea RTI472

在溫室中對常見豆類植物進行研究以確定解澱粉芽孢桿菌RTI472菌株預防及/或減輕植物病原體豆銹病(菜豆銹病菌)及植物病原體灰葡萄孢菌之作用的能力。 Common legume plants were studied in the greenhouse to determine the ability of B. amyloliquefaciens RTI 472 strain to prevent and/or alleviate the effects of plant pathogen bean rust (Nicky rust fungus) and the plant pathogen Botrytis cinerea.

在第一組實驗中，針對葉面施用以控制植物病原體菜豆銹病菌及灰葡萄孢菌，測試解澱粉芽孢桿菌RTI472菌株之不同調配物。除RTI472之外，亦測試另一及新鑑別之解澱粉芽孢桿菌菌株RTI301。實驗設計及調配物如下： In a first set of experiments, different formulations of B. amyloliquefaciens RTI 472 strains were tested for foliar application to control the plant pathogen Phytophthora rust and Botrytis cinerea. In addition to RTI 472, another and newly identified B. amyloliquefaciens strain RTI301 was also tested. The experimental design and formulation are as follows:

調配物：Formulation:

廢醱酵液(SFB)中解澱粉芽孢桿菌RTI472孢子，於水中稀釋約100倍，且以1×108cfu/ml之施用率施用於葉。 The spores of Bacillus amyloliquefaciens RTI472 in waste broth (SFB) were diluted about 100 times in water and applied to the leaves at an application rate of 1 x 10 8 cfu/ml.

廢醱酵液(SFB)中解澱粉芽孢桿菌RTI472孢子，於水中稀釋約100倍且添加酵母提取物且以1×108cfu/ml之施用率及約0.2%酵母提取物施用於葉。 The spores of Bacillus amyloliquefaciens RTI472 in waste broth (SFB) were diluted about 100-fold in water and yeast extract was added and applied to the leaves at an application rate of 1 x 10 8 cfu/ml and about 0.2% yeast extract.

廢醱酵液(SFB)中解澱粉芽孢桿菌RTI301孢子，於水中稀釋約100倍且以1×108cfu/ml之施用率施用於葉 Spores of Bacillus amyloliquefaciens RTI301 in waste broth (SFB), diluted about 100 times in water and applied to leaves at an application rate of 1 × 10 8 cfu/ml

廢醱酵液(SFB)中解澱粉芽孢桿菌RTI301孢子，於水中稀釋約100倍且添加酵母提取物且以1×108cfu/ml之施用率及約0.2%酵母提取物施用於葉。 The spores of Bacillus amyloliquefaciens RTI301 in waste broth (SFB) were diluted about 100-fold in water and yeast extract was added and applied to the leaves at an application rate of 1 x 10 8 cfu/ml and about 0.2% yeast extract.

SERENADE OPTIMUM(BAYER CROP SCIENCE,INC)產品以1×108cfu/ml枯草桿菌菌株QST713孢子之施用率施用。 The SERENADE OPTIMUM (BAYER CROP SCIENCE, INC) product was applied at a rate of application of 1 x 10 8 cfu/ml of B. subtilis strain QST713 spores.

HORIZON(HORIZON AG-PRODUCTS)產品以50g a.i./ha之施用率(得克利)施用。 The HORIZON (HORIZON AG-PRODUCTS) product was applied at an application rate of 50 g a.i./ha (Dickley).

BRAVO WEATHER STIK(SYNGENTA CROP PROTECTION,INC)產品以500g a.i./ha之施用率施用(四氯異苯腈)。 The BRAVO WEATHER STIK (SYNGENTA CROP PROTECTION, INC) product was applied at an application rate of 500 g a.i./ha (tetrachloroisophthalonitrile).

TACTIC(LOVELAND PRODUCTS,INC)產品以0.1875%v/v之濃度包括於所有上列調配物中。 TACTIC (LOVELAND PRODUCTS, INC) products are included in all of the above formulations at a concentration of 0.1875% v/v.

處理施用方法：Treatment application method:

軌道噴霧器(track sprayer)用於用各種上列處理接種21日齡常見豆類植物(具有兩個三葉)，該噴霧器具有單一頂部噴嘴(TeeJet SS8001E平扇形)，壓力=276kPa(40psi)。噴嘴高度為超過豆類植物葉子36cm(14")。施用體積為200L/ha且實驗中重複數目等於六次。處理植物單次接種以及對照植物未接收任何處理。 A track sprayer was used to inoculate 21 day old common legumes (with two trifolials) with various top treatments with a single top nozzle (TeeJet SS8001E flat fan) and pressure = 276 kPa (40 psi). The nozzle height was 36 cm (14") above the leaves of the legumes. The application volume was 200 L/ha and the number of replicates in the experiment was equal to six times. The single treatment of the treated plants and the control plants did not receive any treatment.

感染率：Infection rate:

處理施用一天後，測試植物以200k分生孢子/毫升之接種率感染豆銹病(菜豆銹病菌)。 One day after the treatment, the test plants were infected with bean rust (Nicky rust) at a vaccination rate of 200 k conidia/ml.

感染豆銹病(菜豆銹病菌)九天後，評估以下各者之疾病控制%：單獨用水稀釋之廢醱酵液中RTI472孢子(「RTI472+1%SFB」)、用水稀釋之廢醱酵液中RTI472孢子加上酵母提取物(「RTI472+1%SFB+酵母提取物」)、單獨用水稀釋100倍之廢醱酵液中RTI301孢子 (「RTI301+1%SFB」)、用水稀釋100倍之廢醱酵液中RTI301孢子加上酵母提取物(「RTI301+1%SFB+酵母提取物」)、根據上述施用率之BRAVO WEATHER STIK、HORIZON及SERENADE OPTIMUM。未經處理之對照(僅水)產生28%疾病。 Nine days after infection with bean rust (Nicky rust), the disease control % of each of the following was evaluated: RTI472 spores ("RTI472+1%SFB") in waste broth diluted with water alone, RTI472 in waste broth diluted with water Spore plus yeast extract ("RTI472+1%SFB+yeast extract"), diluted with water 100 times in waste broth in RTI301 spore ("RTI301+1%SFB"), RTI301 spore plus yeast extract ("RTI301+1%SFB+yeast extract") in the waste broth diluted 100 times with water, BRAVO WEATHER STIK, HORIZON according to the above application rate And SERENADE OPTIMUM. An untreated control (water only) produced 28% of the disease.

實驗結果展示於表III中。結果表明對於RTI472及RTI301菌株各者，添加酵母提取物使得與未添加酵母提取物下施用之菌株相比疾病控制增加約40%。當以相同施用率(亦即1×108cfu/ml)施用時，RTI472+1%SFB+酵母提取物及RTI301+1%SFB+酵母提取物展現之疾病控制量類似於針對SERENADE OPTIMUM所觀測之結果，即使RTI472及RTI301調配物中SFB之量相對較低，為1%，且SFB可預期含有具有抗真菌活性之分泌化合物。 The experimental results are shown in Table III. The results indicate that for each of the RTI472 and RTI301 strains, the addition of the yeast extract resulted in an increase in disease control of about 40% compared to the strain administered without the addition of the yeast extract. The RTI472+1%SFB+ yeast extract and RTI301+1%SFB+ yeast extract exhibited disease control levels similar to those observed for SERENADE OPTIMUM when administered at the same application rate (i.e., 1 x 10 8 cfu/ml). Even though the amount of SFB in the RTI 472 and RTI 301 formulations is relatively low, it is 1%, and SFB is expected to contain a secretory compound having antifungal activity.

使用上列相同調配物及實驗設計在胡椒植物中進行之實驗觀測到類似結果，該實驗量測RTI472及RTI301展現之對病原體灰葡萄孢菌所引起之胡椒葡萄孢疫病的疾病控制量(資料未展示)。 Similar results were observed using the same formulation and experimental design described above in pepper plants, which measured RTI 472 and RTI 301 for disease control of Botrytis cinerea caused by the pathogen Botrytis cinerea (data not available) Show).

以下實驗描述RTI472對由植物病原體菜豆銹病菌所引起之豆銹 病的疾病控制。實驗設計及調配物如下： The following experiment describes the RTI472 rust caused by the phytopathogen Bean rust Disease control of the disease. The experimental design and formulation are as follows:

調配物：Formulation:

解澱粉芽孢桿菌RTI472孢子在廢醱酵液(SFB)中，於水中稀釋約100倍且添加酵母提取物且以1×108cfu/ml之施用率及約0.2%酵母提取物施用於葉。 The Bacillus amyloliquefaciens RTI472 spores were diluted about 100-fold in water in spent broth (SFB) and yeast extract was added and applied to the leaves at an application rate of 1 x 10 8 cfu/ml and about 0.2% yeast extract.

SERENADE OPTIMUM(BAYER CROP SCIENCE,INC)產品以1×108cfu/ml及4×108cfu/ml之施用率施用。 The SERENADE OPTIMUM (BAYER CROP SCIENCE, INC) product was applied at an application rate of 1 x 10 8 cfu/ml and 4 x 10 8 cfu/ml.

HORIZON(HORIZON AG-PRODUCTS)以50g a.i./ha之施用率(得克利)施用。 HORIZON (HORIZON AG-PRODUCTS) was applied at an application rate of 50 g a.i./ha (Dickley).

四氯異苯腈以500g a.i./ha之施用率施用。 Tetrachloroisophthalonitrile was applied at an application rate of 500 g a.i./ha.

包括作為空白對照之TACTIC(LOVELAND PRODUCTS,INC)亦以0.1875%v/v之濃度施用至所有調配物。 TACTIC (LOVELAND PRODUCTS, INC), including as a blank control, was also applied to all formulations at a concentration of 0.1875% v/v.

處理施用方法：Treatment application method:

軌道噴霧器用於用各種上列處理接種21日齡常見豆類植物(具有兩個三葉)，該噴霧器具有單一頂部噴嘴(TeeJet SS8001E平扇形)，壓力=276kPa(40psi)。噴嘴高度為超過大豆植物葉子36cm(14")。施用體積為200L/ha且實驗中重複數目等於六次。處理植物單次接種以及對照植物未接收任何處理。 A track sprayer was used to inoculate 21 day old common legumes (with two trifolials) with various top treatments with a single top nozzle (TeeJet SS8001E flat fan) and pressure = 276 kPa (40 psi). The nozzle height was 36 cm (14") above the leaves of the soybean plant. The application volume was 200 L/ha and the number of replicates in the experiment was equal to six times. The single treatment of the treated plants and the control plants did not receive any treatment.

感染率：Infection rate:

處理施用一天後，測試植物以200k分生孢子/毫升之接種率感染豆銹病(菜豆銹病菌)。 One day after the treatment, the test plants were infected with bean rust (Nicky rust) at a vaccination rate of 200 k conidia/ml.

感染豆銹病(菜豆銹病菌)十天後，評估以下各者之疾病控制%：廢醱酵液(SFB)中RTI472孢子(以1×108cfu/ml施用)、得克利(以50g a.i./ha施用)、四氯異苯腈(以50g a.i./ha施用)、SERENADE OPTIMUM(以1×108cfu/ml施用)及SERENADE OPTIMUM(以4×108cfu/ml施用)。亦包括作為對照之TACTIC(以0.1875%施用)施用於所有 調配物。未經處理之對照產生23%疾病(資料未展示)。實驗結果展示在以下表IV及圖3中，且表明與SERENADE OPTIMUM相比，當以相同施用率施用時，豆銹病(菜豆銹病菌)控制之程度類似。 Ten days after infection with soybean rust (Rh. solani), the disease control % of each of the following were evaluated: RTI472 spores (administered at 1 × 10 8 cfu/ml) and declination (with 50 g ai/) in waste broth (SFB) Ha application), tetrachloroisophthalonitrile (administered at 50 g ai/ha), SERENADE OPTIMUM (administered at 1 x 10 8 cfu/ml) and SERENADE OPTIMUM (administered at 4 x 10 8 cfu/ml). TACTIC (administered at 0.1875%) was also included as a control for all formulations. Untreated controls produced 23% of the disease (data not shown). The results of the experiments are shown in Tables IV and 3 below, and show that the degree of control of soybean rust (Natural Bean rust) is similar when applied at the same application rate as compared to SERENADE OPTIMUM.

實例6Example 6 解澱粉芽孢桿菌RTI472對大豆白粉病(白粉病菌)之拮抗作用Antagonistic effect of Bacillus amyloliquefaciens RTI472 on soybean powdery mildew ( powdery mildew)

在溫室中對大豆進行研究以確定解澱粉芽孢桿菌RTI472菌株預防及/或減輕大豆白粉病(白粉病菌)之天然感染作用的能力。 Soybeans were studied in a greenhouse to determine the ability of B. amyloliquefaciens RTI 472 strain to prevent and/or alleviate the natural infection effects of soybean powdery mildew (powder powder).

調配物：Formulation:

解澱粉芽孢桿菌RTI472孢子在廢醱酵液(SFB)中，於水中稀釋約100倍且添加酵母提取物且以1×108cfu/ml之施用率及約0.2%酵母提取物施用於葉。 The Bacillus amyloliquefaciens RTI472 spores were diluted about 100-fold in water in spent broth (SFB) and yeast extract was added and applied to the leaves at an application rate of 1 x 10 8 cfu/ml and about 0.2% yeast extract.

SERENADE OPTIMUM(BAYER CROP SCIENCE,INC)孢子以1×108cfu/ml之施用率施用。 SERENADE OPTIMUM (BAYER CROP SCIENCE, INC) spores were applied at an application rate of 1 x 10 8 cfu/ml.

HORIZON(HORIZON AG-PRODUCTS)以50g a.i./ha之施用率(得克利)施用。 HORIZON (HORIZON AG-PRODUCTS) was applied at an application rate of 50 g a.i./ha (Dickley).

TACTIC(LOVELAND PRODUCTS,INC)以0.1875%v/v之濃度施用至所有調配物。 TACTIC (LOVELAND PRODUCTS, INC) was applied to all formulations at a concentration of 0.1875% v/v.

處理施用方法：Treatment application method:

軌道噴霧器用於用各種處理接種21日齡大豆植物(具有兩個三葉)，該噴霧器具有單一頂部噴嘴(TeeJet SS8001E平扇形)，壓力=276kPa(40psi)。噴嘴高度為超過大豆植物葉子36cm(14")。施用體積為200L/ha且實驗中重複數目等於六次。接受處理之植物單次接種以及對照植物未接收任何處理。 A track sprayer was used to inoculate 21 day old soybean plants (with two trifolials) with various treatments with a single top nozzle (TeeJet SS8001E flat fan) and pressure = 276 kPa (40 psi). The nozzle height was 36 cm (14") above the leaves of the soybean plant. The application volume was 200 L/ha and the number of replicates in the experiment was equal to six. The single inoculation of the treated plants and the control plants did not receive any treatment.

感染率：Infection rate:

處理施用後立即將測試植物置放於先前感染白粉病菌之大豆的平坦部分之間。 Immediately after the treatment, the test plants were placed between the flat portions of the soybeans previously infected with powdery mildew.

置放在白粉病菌感染之平坦部分之間九天後，評估以下各者之疾病控制%：廢醱酵液(SFB)中RTI472孢子(以1×108cfu/ml施用)、SERENADE OPTIMUM(以1×108cfu/ml施用)及HORIZON(得克利以50g a.i./ha施用)。未經處理之對照產生70%疾病(資料未展示)。實驗結果展示在以下表V及圖4中，且表明與SERENADE OPTIMUM相比，當以相同施用率施用時，大豆白粉病(白粉病菌)控制優良。 Nine days after placement between the flat portions of the powdery mildew infection, the disease control % of each of the following were evaluated: RTI472 spores (administered at 1 × 10 8 cfu/ml), SERENADE OPTIMUM in waste broth (SFB) ×10 8 cfu/ml application) and HORIZON (administered at 50 g ai/ha). Untreated controls produced 70% of the disease (data not shown). The results of the experiment are shown in Table V below and in Figure 4, and indicate that soybean powdery mildew (Saccharomyces cerevisiae) is excellently controlled when applied at the same application rate as compared with SERENADE OPTIMUM.

圖4中之圖展示在用以下各者之處理接種9天之後y軸上疾病控制%(平均值)：廢醱酵液(SFB)中RTI472孢子(以1×108cfu/ml施用)、SERENADE OPTIMUM(以1×108cfu/ml施用)及HORIZON(得克利以50g a.i./ha施用)。檢查對照產生70%疾病(資料未展示)。 The graph in Figure 4 shows the % disease control on the y-axis after 9 days of inoculation with the following treatments: average RTI472 spores (administered at 1 x 10 8 cfu/ml) in spent broth (SFB), SERENADE OPTIMUM (administered at 1 x 10 8 cfu/ml) and HORIZON (administered at 50 g ai/ha). Check the control to produce 70% of the disease (data not shown).

實例7Example 7 解澱粉芽孢桿菌RTI472對胡椒葡萄孢疫病(灰葡萄孢菌)之拮抗作用Antagonistic effect of Bacillus amyloliquefaciens RTI472 on Botrytis cinerea (Botrytis cinerea)

在溫室中對胡椒進行研究以確定解澱粉芽孢桿菌RTI472菌株預防及/或減輕植物病原體胡椒葡萄孢疫病(灰葡萄孢菌)之作用的能力。 Pepper was studied in a greenhouse to determine the ability of B. amyloliquefaciens RTI 472 strain to prevent and/or alleviate the effects of the plant pathogen, Botrytis cinerea (B. cinerea).

調配物：Formulation:

解澱粉芽孢桿菌RTI472孢子在廢醱酵液(SFB)中，於水中稀釋約100倍且添加酵母提取物且以1×108cfu/ml之施用率及約0.2%酵母提取物施用於葉。 The Bacillus amyloliquefaciens RTI472 spores were diluted about 100-fold in water in spent broth (SFB) and yeast extract was added and applied to the leaves at an application rate of 1 x 10 8 cfu/ml and about 0.2% yeast extract.

SERENADE OPTIMUM(BAYER CROP SCIENCE,INC)孢子以1×108cfu/ml及4×108cfu/ml之施用率施用。 SERENADE OPTIMUM (BAYER CROP SCIENCE, INC) spores were applied at an application rate of 1 x 10 8 cfu/ml and 4 x 10 8 cfu/ml.

HORIZON(HORIZON AG-PRODUCTS)以50g a.i./ha之施用率(得克利)施用。 HORIZON (HORIZON AG-PRODUCTS) was applied at an application rate of 50 g a.i./ha (Dickley).

四氯異苯腈以500g a.i./ha之施用率施用。 Tetrachloroisophthalonitrile was applied at an application rate of 500 g a.i./ha.

包括作為空白對照之TACTIC(LOVELAND PRODUCTS,INC)亦以0.1875%v/v之濃度施用至所有調配物。 TACTIC (LOVELAND PRODUCTS, INC), including as a blank control, was also applied to all formulations at a concentration of 0.1875% v/v.

處理施用方法：Treatment application method:

軌道噴霧器用於用各種處理接種28日齡胡椒植物，該噴霧器具有單一頂部噴嘴(TeeJet SS8001E平扇形)，壓力=276kPa(40psi)。噴嘴高度為超過胡椒植物葉子36cm(14")。施用體積為200L/ha且實驗中重複數目等於六次。處理植物單次接種以及對照植物未接收任何處理。 A track sprayer was used to inoculate 28 day old pepper plants with various treatments with a single top nozzle (TeeJet SS8001E flat sector) and pressure = 276 kPa (40 psi). The nozzle height was 36 cm (14") above the pepper plant leaves. The application volume was 200 L/ha and the number of replicates in the experiment was equal to six times. The single treatment of the treated plants and the control plants did not receive any treatment.

感染率：Infection rate:

處理施用一天後，測試植物以1M分生孢子/毫升之感染率感染灰葡萄孢菌。 One day after the treatment, the test plants were infected with Botrytis cinerea at an infection rate of 1 M conidia/ml.

用胡椒葡萄孢疫病(灰葡萄孢菌)感染七天後，評估以下各者之疾病控制%：廢醱酵液(SFB)中RTI472孢子(以1×108cfu/ml施用)、 TACTIC(以0.1875%施用)、得克利(以50g a.i./ha施用)(四氯異苯腈以500g a.i./ha施用)、SERENADE OPTIMUM(以1×108cfu/ml施用)及SERENADE OPTIMUM(以4×108cfu/ml施用)。未經處理之對照產生6%疾病(資料未展示)。實驗結果展示在以下表VI及圖5中，且表明與SERENADE OPTIMUM相比，當以相同施用率施用時，胡椒葡萄孢疫病(灰葡萄孢菌)控制優良。 After seven days of infection with Botrytis cinerea (B. cinerea), the disease control % of each of the following were evaluated: RTI472 spores (administered at 1 × 10 8 cfu/ml), TACTIC (at 0.1875) in waste broth (SFB) % application), Dekli (administered at 50 g ai/ha) (tetrachloroisophthalonitrile applied at 500 g ai/ha), SERENADE OPTIMUM (administered at 1×10 8 cfu/ml) and SERENADE OPTIMUM (at 4×10 8 Cfu/ml application). Untreated controls produced 6% of the disease (data not shown). The results of the experiment are shown in Table VI below and in Figure 5, and indicate that Botrytis cinerea (B. cinerea) is excellently controlled when applied at the same application rate as compared to SERENADE OPTIMUM.

在另一實驗中，在溫室中對胡椒進行研究以確定解澱粉芽孢桿菌RTI472菌株預防及/或減輕植物病原體胡椒葡萄孢疫病(灰葡萄孢菌)作用之理想劑量。 In another experiment, pepper was studied in a greenhouse to determine the optimal dose of B. amyloliquefaciens RTI 472 strain to prevent and/or alleviate the effects of the plant pathogen, Botrytis cinerea (B. cinerea).

調配物：Formulation:

解澱粉芽孢桿菌RTI472孢子廢醱酵液(SFB)中且添加酵母提取物且以在2.5×106cfu/ml至2.5×108cfu/ml及0.01%至0.2%酵母提取物範圍內之施用量施用於葉。 Bacillus amyloliquefaciens RTI472 spore waste mash (SFB) and added yeast extract and applied in the range of 2.5×10 6 cfu/ml to 2.5×10 8 cfu/ml and 0.01% to 0.2% yeast extract The amount is applied to the leaves.

SERENADE OPTIMUM(BAYER CROP SCIENCE,INC)孢子以在2.5×106cfu/ml至4×108cfu/ml範圍內之施用率使用。 SERENADE OPTIMUM (BAYER CROP SCIENCE, INC) spores were used at an application rate ranging from 2.5 x 10 6 cfu/ml to 4 x 10 8 cfu/ml.

HORIZON(HORIZON AG-PRODUCTS)以50g a.i./ha之施用率(得克利)施用。 HORIZON (HORIZON AG-PRODUCTS) was applied at an application rate of 50 g a.i./ha (Dickley).

亦包括作為空白對照之TACTIC(LOVELAND PRODUCTS,INC)亦以0.1875%v/v之濃度施用至所有調配物。 TACTIC (LOVELAND PRODUCTS, INC), also included as a blank control, was also applied to all formulations at a concentration of 0.1875% v/v.

處理施用方法：Treatment application method:

軌道噴霧器用於用各種處理接種28日齡胡椒植物，該噴霧器具有單一頂部噴嘴(TeeJet SS8001E平扇形)，壓力=276kPa(40psi)。噴嘴高度為超過胡椒植物葉子36cm(14")。施用體積為200L/ha且實驗中重複數目等於六次。處理植物單次接種以及對照植物未接收任何處理。 A track sprayer was used to inoculate 28 day old pepper plants with various treatments with a single top nozzle (TeeJet SS8001E flat sector) and pressure = 276 kPa (40 psi). The nozzle height was 36 cm (14") above the pepper plant leaves. The application volume was 200 L/ha and the number of replicates in the experiment was equal to six times. The single treatment of the treated plants and the control plants did not receive any treatment.

感染率：Infection rate:

處理施用一天後，測試植物以1M分生孢子/毫升之接種率感染灰葡萄孢菌。 One day after the treatment, the test plants were infected with Botrytis cinerea at a vaccination rate of 1 M conidia/ml.

在感染胡椒葡萄孢疫病(灰葡萄孢菌)三天後，評估以下各者之疾病控制%：與TACTIC(以0.1875%施用於所有調配物且亦用作空白對照)、SERENADE OPTIMUM(以4×108施用)及得克利(以50g a.i/ha施用)相比，廢醱酵液(SFB)中RTI472孢子(以2.5×106、1×107、2.5×107、1×108及2.5×108cfu/ml施用)及SERENADE OPTIMUM(以2.5×106、1×107、2.5×107、1×108及2.5×108cfu/ml施用)。未經處理之對照產生70%疾病(資料未展示)。實驗結果展示在以下表VII及圖6中。 Three days after infection with Botrytis cinerea (Botrytis cinerea), % of disease control was evaluated for each of the following: with TACTIC (administered to all formulations at 0.1875% and also used as a blank), SERENADE OPTIMUM (with 4×) 10 8 application) and Dekeley (administered at 50 g ai/ha) compared to RTI472 spores in waste broth (SFB) (with 2.5 × 10 6 , 1 × 10 7 , 2.5 × 10 7 , 1 × 10 8 and 2.5×10 8 cfu/ml application) and SERENADE OPTIMUM (administered at 2.5×10 6 , 1×10 7 , 2.5×10 7 , 1×10 8 and 2.5×10 8 cfu/ml). Untreated controls produced 70% of the disease (data not shown). The experimental results are shown in Table VII and Figure 6 below.

在另一實驗中，在溫室中對胡椒進行研究以確定在變化病原體接種率下解澱粉芽孢桿菌RTI472菌株預防及/或減輕植物病原體胡椒葡萄孢疫病(灰葡萄孢菌)作用之能力。 In another experiment, pepper was studied in a greenhouse to determine the ability of the B. amyloliquefaciens RTI472 strain to prevent and/or alleviate the effects of the plant pathogen Botrytis blight (Botrytis cinerea) at varying pathogen inoculation rates.

調配物：Formulation:

解澱粉芽孢桿菌RTI472孢子在廢醱酵液(SFB)中，於水中稀釋約100倍且添加酵母提取物且以1×108cfu/ml之施用率及約0.2%酵母提取物施用於葉。 The Bacillus amyloliquefaciens RTI472 spores were diluted about 100-fold in water in spent broth (SFB) and yeast extract was added and applied to the leaves at an application rate of 1 x 10 8 cfu/ml and about 0.2% yeast extract.

SERENADE OPTIMUM(BAYER CROP SCIENCE,INC)孢子以1×108cfu/ml至4×108cfu/ml之施用率使用。 SERENADE OPTIMUM (BAYER CROP SCIENCE, INC) spores were used at an application rate of 1 x 10 8 cfu/ml to 4 x 10 8 cfu/ml.

HORIZON(HORIZON AG-PRODUCTS)以50g a.i./ha之施用率(得克利)施用。 HORIZON (HORIZON AG-PRODUCTS) was applied at an application rate of 50 g a.i./ha (Dickley).

亦包括作為空白對照之TACTIC(LOVELAND PRODUCTS,INC)亦以0.1875%v/v之濃度施用至所有調配物。 TACTIC (LOVELAND PRODUCTS, INC), also included as a blank control, was also applied to all formulations at a concentration of 0.1875% v/v.

處理施用方法：Treatment application method:

軌道噴霧器用於用各種處理接種28日齡胡椒植物，該噴霧器具有單一頂部噴嘴(TeeJet SS8001E平扇形)，壓力=276kPa(40psi)。噴嘴高度為超過胡椒植物葉子36cm(14")。施用體積為200L/ha且實驗中重複數目等於六次。處理植物單次接種以及對照植物未接收任何處理。 A track sprayer was used to inoculate 28 day old pepper plants with various treatments with a single top nozzle (TeeJet SS8001E flat sector) and pressure = 276 kPa (40 psi). The nozzle height was 36 cm (14") above the pepper plant leaves. The application volume was 200 L/ha and the number of replicates in the experiment was equal to six times. The single treatment of the treated plants and the control plants did not receive any treatment.

感染率：Infection rate:

處理施用後一天，測試植物以50k至2M分生孢子/毫升範圍內之接種率感染灰葡萄孢菌。 One day after the treatment, the test plants were infected with Botrytis cinerea at an inoculation rate ranging from 50 k to 2 M conidia/ml.

在感染胡椒葡萄孢疫病(灰葡萄孢菌)四天後，評估以下各者之疾病控制%：與TACTIC(以0.1875%施用於所有調配物且亦用作空白對 照)及得克利(HORIZON；以50g a.i/ha施用)相比，廢醱酵液(SFB)中RTI472孢子(以2.5×106cfu/ml施用)及SERENADE OPTIMUM(以1×108及4×108cfu/ml施用)。未經處理之對照中疾病%(資料未展示)為50k=15%，100k=30%，500k=65%，1M=65%，且2M=65%。實驗結果展示在以下表VIII及圖7中。另外，圖8A-8D展示在灰葡萄孢菌50k接種率下植物之影像，圖9A-9D展示灰葡萄孢菌100k接種率下植物之影像，圖10A-10D展示灰葡萄孢菌500k接種率下植物之影像，圖11A-11D展示灰葡萄孢菌1M接種率下植物之影像，且圖12A-12D展示灰葡萄孢菌2M接種率下植物之影像。在3個最低病原體接種率下，觀測到RTI472菌株及SERENADE OPTIMUM之疾病控制百分比類似。然而，觀測到與SERENADE OPTIMUM相比，在2個最高病原體接種率1M及2M下，經RTI472菌株處理之植物達到統計學改善。 Four days after infection with Botrytis cinerea (B. cinereus), % of disease control was assessed for each of the following: with TACTIC (administered to all formulations at 0.1875% and also used as a blank) and Kelly (HORIZON; 50 g ai/ha application) RTI 472 spores (administered at 2.5 x 10 6 cfu/ml) and SERENADE OPTIMUM (administered at 1 x 10 8 and 4 x 10 8 cfu/ml) in spent broth (SFB). The % of disease in the untreated control (data not shown) was 50k = 15%, 100k = 30%, 500k = 65%, 1M = 65%, and 2M = 65%. The experimental results are shown in Table VIII and Figure 7 below. In addition, Figures 8A-8D show images of plants under the 50k inoculation rate of Botrytis cinerea, Figures 9A-9D show images of plants under the 100k inoculation rate of Botrytis cinerea, and Figures 10A-10D show the inoculation rate of Botrytis cinerea 500k. Images of plants, Figures 11A-11D show images of plants under the 1M inoculation rate of Botrytis cinerea, and Figures 12A-12D show images of plants under the 2M inoculation rate of Botrytis cinerea. At the three lowest pathogen inoculation rates, the percentage of disease control observed for RTI 472 strain and SERENADE OPTIMUM was similar. However, it was observed that plants treated with the RTI 472 strain achieved statistical improvement at the highest vaccination rates of 1 M and 2 M compared to SERENADE OPTIMUM.

實例8Example 8 解澱粉芽孢桿菌RTI472對葫蘆中白粉病之拮抗作用Antagonistic effect of Bacillus amyloliquefaciens RTI472 on powdery mildew in gourd

研究在佛羅里達州(Florida)之葫蘆田間試驗中進行以確定解澱粉芽孢桿菌RTI472菌株預防及/或減輕植物病原體葫蘆疾病白粉病作用 的能力。 The study was conducted in the field trial of the gourd in Florida to determine the prevention and/or alleviation of the powdery mildew effect of the plant pathogen cucurbit disease RTI472 strain. Ability.

整體上，使用如以下表IX中所指示之施用率，各產品進行6次施用。在RTI472之情況下，施用率以g/ha為單位且施用率對應於與基於1400g/ha施用率，針對SERENADE OPTIMUM所推薦相同之菌落形成單位/公頃。 Overall, each product was applied 6 times using an application rate as indicated in Table IX below. In the case of RTI 472, the application rate is in g/ha and the application rate corresponds to the same colony forming unit/ha as recommended for SERENADE OPTIMUM based on the application rate of 1400 g/ha.

除非另外說明，否則所有施用進行6次，施用之間的時間間隔為5至7天。第一次施用之時間安排視特定作物而定且在種植時、作物萌芽後幾週、開花時、出現疾病時或預期疾病即將出現前的範圍內。在將生物活性成分與化學活性成分之施用組合的方案4、5及6之情況下，用生物活性成分進行第一、第三及第五次施用(A、C及E)，而用化學活性成分進行第二、第四及第六次施用(B、D及F)。 All applications were performed 6 times, with a time interval between administrations of 5 to 7 days, unless otherwise stated. The timing of the first application depends on the particular crop and is within the range of the crop, the weeks after the crop is germinated, the flowering, the disease, or the expected disease. In the case of Schemes 4, 5 and 6 in which the bioactive ingredient is combined with the administration of the chemically active ingredient, the first, third and fifth administrations (A, C and E) are carried out with the biologically active ingredient, while chemically active The ingredients were administered in the second, fourth and sixth applications (B, D and F).

調配物：Formulation:

SERENADE OPTIMUM(BAYER CROP SCIENCE,INC)以1400g/ha，對應於1.8×10+13CFU/ha之施用率施用。 SERENADE OPTIMUM (BAYER CROP SCIENCE, INC ) to 1400g / ha, corresponding to 1.8 × 10 +13 CFU / ha, of administration.

解澱粉芽孢桿菌RTI472孢子在廢醱酵液(SFB)中且添加酵母提取物，且施用率對應於與基於1400g/ha施用率，針對SERENADE OPTIMUM所推薦相同之菌落形成單位/公頃(CFU/ha)且酵母提取物在約0.01%至0.2%範圍內。 Bacillus amyloliquefaciens RTI472 spores in spent broth (SFB) and added yeast extract, and the application rate corresponds to the same colony forming unit/ha (CFU/ha) as recommended for SERENADE OPTIMUM based on 1400 g/ha application rate And the yeast extract is in the range of about 0.01% to 0.2%.

TACTIC(LOVELAND PRODUCTS,INC)以0.1875%v/v之濃度施用。 TACTIC (LOVELAND PRODUCTS, INC) was applied at a concentration of 0.1875% v/v.

LUNA EXPERIENCE(BAYER CROP SCIENCE,INC)以500g a.i./ha之施用率施用(氟吡菌醯胺加上得克利殺真菌劑)。 LUNA EXPERIENCE (BAYER CROP SCIENCE, INC) was applied at an application rate of 500 g a.i./ha (fluopyram and plus kle fungicide).

BRAVO WEATHER STIK(SYNGENTA CROP PROTECTION,INC)以2240g a.i./ha之施用率施用(四氯異苯腈)。 BRAVO WEATHER STIK (SYNGENTA CROP PROTECTION, INC) was applied at an application rate of 2240 g a.i./ha (tetrachloroisophthalonitrile).

實驗設計如下：未經處理之對照、RTI472+TACTIC、SERENADE OPTIMUM+TACTIC、RTI472+LUNA EXPERIENCE+ TACTIC、SERENADE OPTIMUM+LUNA EXPERIENCE+TACTIC及BRAVO WEATHER STIK+LUNA EXPERIENCE+TACTIC。 The experimental design is as follows: untreated control, RTI472+TACTIC, SERENADE OPTIMUM+TACTIC, RTI472+LUNA EXPERIENCE+ TACTIC, SERENADE OPTIMUM+LUNA EXPERIENCE+TACTIC and BRAVO WEATHER STIK+LUNA EXPERIENCE+TACTIC.

處理施用方法：Treatment application method:

如上所述按時間安排進行六次施用。施用噴霧器設定為輸送30加侖/英畝。個別小塊土地使用具有平扇形噴嘴(8004類型)之CO2背包式噴霧器以4mph之地速噴灑且各噴嘴相隔18吋。輸送化學品之載劑為混合在2公升瓶中之水。 Six applications were performed on a schedule as described above. The sprayer was set to deliver 30 gallons per acre. Individual plots were sprayed at a ground speed of 4 mph using a CO 2 backpack sprayer with a flat fan nozzle (type 8004) and each nozzle was 18 inches apart. The carrier that transports the chemical is water mixed in a 2 liter bottle.

疾病評分：針對各處理，評估植物之5片葉子之疾病嚴重程度平均%。評估來自各處理之四個小塊土地的作物反應及白粉病之疾病控制。在各施用之後，即將下一次施用前，將試驗評級。白粉病來自天然感染。黃瓜之實驗結果展示在以下表IX中且表明與SERENADE OPTIMUM相比，當以相同施用率施用時，作為單獨生物殺真菌劑或作為處理方案之一部分，黃瓜中白粉病之控制類似。 Disease score: For each treatment, the average degree of disease severity of the 5 leaves of the plants was evaluated. Evaluation of crop response and disease control of powdery mildew from four plots of each treatment. After each application, the test will be rated just prior to the next application. Powdery mildew comes from natural infections. The results of the cucumber test are shown in Table IX below and indicate that the control of powdery mildew in cucumber is similar when applied at the same application rate as part of a single biofungicide or as part of a treatment regimen compared to SERENADE OPTIMUM.

在冬南瓜及黃瓜中在商業生產溫室田間試驗中使用相同格式及施用率進行類似實驗，以確定RTI472菌株預防及/或減輕植物病原體白粉病之作用的能力。對於黃瓜及冬南瓜，在作物萌芽之後約3週進行第一次處理施用。下文分別在表X及XI中展示冬南瓜及黃瓜之資料。表示為「疾病壓力曲線下面積」(AUDPC)之疾病嚴重程度作為上葉表面上白粉病之嚴重程度的函數(受影響葉面積%)確定。 Similar experiments were carried out in a commercial field greenhouse field trial in winter squash and cucumber using the same format and application rate to determine the ability of the RTI 472 strain to prevent and/or alleviate the effects of plant pathogen powdery mildew. For cucumber and winter squash, the first treatment application was carried out about 3 weeks after the crop was germinated. Information on winter squash and cucumber is shown in Tables X and XI below. The severity of the disease expressed as "area under the disease stress curve" (AUDPC) was determined as a function of the severity of powdery mildew on the upper leaf surface (% of affected leaf area).

與SERENADE OPTIMUM相比，RTI472作為單獨生物殺真菌劑，更佳地控制冬南瓜上之白粉病。針對三個方案，觀測到冬南瓜上白粉病之最佳控制，其中解澱粉芽孢桿菌RTI472與LUNA EXPERIENCE(BAYER CROP SCIENCE)之組合等於基於使用BRAVO WEATHER STIK(SYNGENTA)與LUNA EXPERIENCE組合之行業標準方案。因此，解澱粉芽孢桿菌RTI472可用作用於控制葫蘆上白粉病之方案中四氯異苯腈殺真菌劑之替代。在控制黃瓜上白粉病之試驗中進一步證實此，其中單獨之解澱粉芽孢桿菌RTI472展示與使用行業標準LUNA EXPERIENCE之方案類似的效能(參見表XI)。 Compared to SERENADE OPTIMUM, RTI472 acts as a separate biofungicide to better control powdery mildew on winter squash. The optimal control of powdery mildew on winter squash was observed for three scenarios, in which the combination of B. amyloliquefaciformis RTI472 and LUNA EXPERIENCE (BAYER CROP SCIENCE) was equivalent to an industry standard scheme based on the combination of BRAVO WEATHER STIK (SYNGENTA) and LUNA EXPERIENCE. . Therefore, B. amyloliquefaciens RTI 472 can be used as an alternative to the tetrachloroisophthalonitrile fungicide in the scheme for controlling powdery mildew on the gourd. This was further confirmed in an experiment to control powdery mildew on cucumber, in which B. amyloliquefaciens RTI472 showed similar efficacy to the protocol using the industry standard LUNA EXPERIENCE (see Table XI).

實例9Example 9 解澱粉芽孢桿菌RTI472對食莢菜豆及花生中白黴及葉斑病之拮抗作用Antagonistic effect of Bacillus amyloliquefaciens RTI472 on white mold and leaf spot in pod bean and peanut

在食莢菜豆及花生之田間試驗中進行研究以確定解澱粉芽孢桿菌RTI472菌株預防及/或減輕由食莢菜豆中向日葵核盤菌及花生中葉斑病所引起的植物病原體棉狀腐病(白黴)之作用之能力。 In the field trials of pods and peanuts, the Bacillus amyloliquefaciens RTI472 strain was used to prevent and/or alleviate the phytopathogenic cotton rot caused by the leaf spot of sunflower sclerotiorum and peanut leaf spot in pods (white The ability of the mold to act.

除非另外說明，否則整體上，使用如以下表XII中所指示之施用率，各產品進行6次施用，其中施用之間的時間間隔為5至7天。第一次施用之時間安排視特定作物而定且在種植時、作物萌芽後幾週、開花時、出現疾病時或預期疾病即將出現前的範圍內。在RTI472(*施用量，g/ha)之情況下，施用率對應於與基於1400g/ha施用率，針對SERENADE OPTIMUM所推薦相同之菌落形成單位/公頃。在將生物活性成分與化學活性成分之施用組合的方案4、5及6之情況下，用生物活性成分進行第一、第三及第五次施用(A、C及E)，而用化學活性成分進行第二、第四及第六次施用(B、D及F)。 Unless otherwise stated, overall, each product was applied 6 times using an application rate as indicated in Table XII below, with a time interval between administrations of 5 to 7 days. The timing of the first application depends on the particular crop and is within the range of the crop, the weeks after the crop is germinated, the flowering, the disease, or the expected disease. In the case of RTI 472 (* application amount, g/ha), the application rate corresponds to the same colony forming unit/ha as recommended for SERENADE OPTIMUM based on the application rate of 1400 g/ha. In the case of Schemes 4, 5 and 6 in which the bioactive ingredient is combined with the administration of the chemically active ingredient, the first, third and fifth administrations (A, C and E) are carried out with the biologically active ingredient, while chemically active The ingredients were administered in the second, fourth and sixth applications (B, D and F).

調配物：Formulation:

SERENADE OPTIMUM(BAYER CROP SCIENCE,INC)以1400g/ha，對應於1.8×10+13CFU/ha之施用率施用。 SERENADE OPTIMUM (BAYER CROP SCIENCE, INC ) to 1400g / ha, corresponding to 1.8 × 10 +13 CFU / ha, of administration.

解澱粉芽孢桿菌RTI472孢子在廢醱酵液(SFB)中且添加酵母提取物，且施用率對應於與基於1400g/ha施用率，針對SERENADE OPTIMUM所推薦相同之菌落形成單位/公頃，且酵母提取物在約0.01%至0.2%範圍內。 Bacillus amyloliquefaciens RTI472 spores in spent broth (SFB) and added yeast extract, and the application rate corresponds to an application rate based on 1400 g/ha for SERENADE The same colony forming units/ha are recommended by OPTIMUM, and the yeast extract is in the range of about 0.01% to 0.2%.

TACTIC(LOVELAND PRODUCTS,INC)以0.1875%v/v之濃度施用。 TACTIC (LOVELAND PRODUCTS, INC) was applied at a concentration of 0.1875% v/v.

TOPSIN M 70W(CEREXAGRI,INC.)以1570g a.i./ha(甲基托布津殺真菌劑)之施用率施用。 TOPSIN M 70W (CEREX AGRI, INC.) was applied at an application rate of 1570 g a.i./ha (methyl thiophanate fungicide).

PROLINE(BAYER CROP SCIENCE)以200g a.i./ha(丙硫菌唑殺真菌劑)之施用率施用。 PROLINE (BAYER CROP SCIENCE) was applied at an application rate of 200 g a.i./ha (propionazole fungicide).

實驗設計如下：未經處理之對照、RTI472+TACTIC、SERENADE OPTIMUM+TACTIC、RTI472+TOPSIN M 70W+TACTIC、SERENADE OPTIMUM+TOPSIN M 70W+TACTIC及PROLINE+TOPSIN M 70W+TACTIC。 The experimental design is as follows: untreated control, RTI472+TACTIC, SERENADE OPTIMUM+TACTIC, RTI472+TOPSIN M 70W+TACTIC, SERENADE OPTIMUM+TOPSIN M 70W+TACTIC and PROLINE+TOPSIN M 70W+TACTIC.

處理施用方法：除非另外說明，否則所有施用均進行6次，其中施用之間的時間間隔為5至7天。施用噴霧器設定為輸送30加侖/英畝。個別小塊土地使用具有平扇形噴嘴(8004類型)之CO2背包式噴霧器以4mph之地速噴灑且各噴嘴相隔18吋。輸送化學品之載劑為混合在2公升瓶中之水。 Treatment Method of Administration: All applications were performed 6 times, unless otherwise stated, with a time interval between administrations of 5 to 7 days. The sprayer was set to deliver 30 gallons per acre. Individual plots were sprayed at a ground speed of 4 mph using a CO 2 backpack sprayer with a flat fan nozzle (type 8004) and each nozzle was 18 inches apart. The carrier that transports the chemical is water mixed in a 2 liter bottle.

疾病評分： Disease score:

食莢菜豆中白黴試驗：莖幹評估-每個小塊土地評估十個莖幹。疾病嚴重程度藉由視覺估計受疾病影響之各莖幹之量來確定。評估植物上樹冠-葉子、發育中及成熟之果實。每個小塊土地六個2呎區段評估為子樣品且嚴重程度藉由評估展示白黴症狀之樹冠百分比來確定。評估整個樹冠。 White mold test in pods: stem evaluation - Ten stems were evaluated for each plot. The severity of the disease is determined by visually estimating the amount of stems affected by the disease. Evaluate the canopy-leaf, developing and mature fruit on the plant. Six of the two plots of each plot were evaluated as subsamples and the severity was determined by assessing the percentage of canopy showing the symptoms of white mold. Evaluate the entire canopy.

實驗結果展示於以下表XII中。與SERENADE OPTIMUM相比，RTI472作為單獨生物殺真菌劑，更佳地控制食莢菜豆上之白黴。觀測到使用解澱粉芽孢桿菌RTI472與TOPSIN(甲基托布津殺真菌劑)之組 合的方案對食莢菜豆上白黴控制最佳，其甚至比基於使用PROLINE(丙硫菌唑殺真菌劑)與TOPSIN組合之化學方案更佳。因此，解澱粉芽孢桿菌RTI472可用於方案中作為丙硫菌唑殺真菌劑之替代，以控制在乾豆、芥花、玉米、花生、大豆及甜菜上發現之諸如尾孢菌葉斑病、殼針孢褐斑病、核盤菌屬及絲核菌之疾病。 The experimental results are shown in Table XII below. Compared to SERENADE OPTIMUM, RTI472 acts as a separate biofungicide to better control the white mold on the pod bean. A group using B. amyloliquefaciens RTI472 and TOPSIN (methyl thiophanate fungicide) was observed. The combination scheme is optimal for white mold on the pod, which is even better than the chemical scheme based on the combination of PROLINE (propionazole fungicide) and TOPSIN. Therefore, B. amyloliquefaciens RTI472 can be used as an alternative to prothioconazole fungicides in the program to control such as Cercospora leaf spot, shell found on dried beans, canola, corn, peanuts, soybeans and beets. Diseases of needle brown spot, Sclerotinia and Rhizoctonia.

在喬治亞州(Georgia)之花生田間試驗中進行類似研究以確定解澱粉芽孢桿菌RTI472菌株預防及/或減輕花生中植物病原體南方白黴及由尾孢菌/尾孢菌所引起之葉斑病天然出現之作用的能力。如上所述之相同施用率用於RTI472及SERENADE OPTIMUM且使用相同格式，其中各菌株分開施用6次，其中施用之間的時間間隔為5至7天。莖幹在食莢菜豆之白黴試驗中進行評估。每個小塊土地樹冠六個2呎區段評估為子樣品且嚴重程度藉由自整個樹冠評估展示症狀之葉百分比來確定。疾病嚴重程度評分為每1m列白色黴菌之命中數目且葉斑病指數依1至10等級評分。將所有花生收穫，計數，稱重，且分成可銷售 或患病，以確定每英畝之產量。 A similar study was conducted in a peanut field trial in Georgia to determine that the B. amyloliquefaciens RTI472 strain prevents and/or alleviates the plant pathogen P. perforatum in peanuts and the leaf spot caused by Cercospora/Cervium The ability to appear. The same application rates as described above were used for RTI 472 and SERENADE OPTIMUM and the same format was used, with each strain applied 6 times separately with a time interval between administrations of 5 to 7 days. The stems were evaluated in the white mold test of the pods. The six 2-inch segments of each small land canopy were evaluated as subsamples and the severity was determined by evaluating the percentage of leaves showing symptoms from the entire canopy. The severity of the disease was scored as the number of hits per 1 m column of white mold and the leaf spot index was scored on a scale of 1 to 10. Harvest, count, weigh, and divide all peanuts into saleable Or sick to determine the yield per acre.

下文分別在表XIII及XIV中展示中度疾病壓力及重度疾病壓力之實驗結果。RTI472比SERENADE OPTIMUM及未經處理之對照更好地控制花生葉斑病。在兩個試驗中觀測此等結果，一個試驗具有中度壓力且一個試驗具有重度壓力。RTI472比SERENADE OPTIMUM及未經處理之對照更好地遏制花生南方白黴。在兩個試驗中觀測此等結果，一個試驗具有中度壓力且一個試驗具有重度壓力。在包括六次施用時間安排之噴霧方案期間未觀測到作物對RTI472之陰性反應。兩種疾病存在於田間之花生產量展示用RTI472處理引起產量反應高於SERENADE OPTIMUM及未經處理之對照。 Experimental results of moderate disease stress and severe disease stress are shown below in Tables XIII and XIV, respectively. RTI472 better controls peanut leaf spot than SERENADE OPTIMUM and untreated controls. These results were observed in two experiments, one with moderate pressure and one with severe pressure. RTI 472 better detoxifies P. chinensis than SERENADE OPTIMUM and untreated controls. These results were observed in two experiments, one with moderate pressure and one with severe pressure. No negative reaction of the crop to RTI 472 was observed during the spray protocol including six application schedules. The peanut yields of both diseases present in the field were treated with RTI 472 to cause a yield response higher than SERENADE OPTIMUM and untreated controls.

實例10Example 10 解澱粉芽孢桿菌RTI472對小麥赤黴病、大豆銹病及立枯交鏈孢菌之拮抗作用Antagonistic effect of Bacillus amyloliquefaciens RTI472 on wheat scab, soybean rust and Alternaria alternata

在喬治亞州之小麥、大豆及番茄田間試驗中進行研究以確定解澱粉芽孢桿菌RTI472菌株預防及/或減輕植物病原體小麥赤黴病、大豆銹病及立枯交鏈孢菌之作用的能力。 Studies were conducted in the wheat, soybean, and tomato field trials in Georgia to determine the ability of B. amyloliquefaciens RTI472 to prevent and/or alleviate the effects of plant pathogens such as wheat scab, soybean rust, and Alternaria alternata.

SERENADE OPTIMUM使用1400g/ha之施用率，且RTI472使用對應於與基於1400g/ha施用率，針對SERENADE OPTIMUM所推薦相同之菌落形成單位/公頃的施用率，施用RTI472及SERENADE OPTIMUM各者。除非另外說明，否則處理施用輸送至作物，施用之間的時間間隔為5至7天。施用次數及第一次施用之時間安排視特定作物而定且在種植時、作物萌芽後幾週、開花時、出現疾病時或預期疾病即將出現前的範圍內。施用噴霧器設定為輸送20加侖/英畝(189l/ha)。個別小塊土地使用具有成對平扇形噴嘴(8003類型)之CO2背包式噴霧器以3mph之地速噴灑且各噴嘴相隔18吋。輸送化學品之載劑為混合在2公升瓶中之水。 The SERENADE OPTIMUM used an application rate of 1400 g/ha, and the RTI 472 was applied to each of the RTI 472 and SERENADE OPTIM UM using an application rate corresponding to the same colony forming unit/ha as recommended by SERENADE OPTIMUM based on the 1400 g/ha application rate. Unless otherwise stated, the treatment application is delivered to the crop with a time interval between administration of 5 to 7 days. The number of applications and the timing of the first application are dependent on the particular crop and are within the range of the crop, the weeks after the crop germination, the flowering, the onset of the disease, or the pre-expected disease. The sprayer was set to deliver 20 gallons per acre (189 l/ha). Individual plots were sprayed at 3 mph using a CO 2 backpack sprayer with pairs of flat fan nozzles (type 8003) and the nozzles were 18 angstroms apart. The carrier that transports the chemical is water mixed in a 2 liter bottle.

對於小麥赤黴病，在作物開花時進行單次處理施用。處理三天後，植物用赤黴病病原體玉米赤黴(亦稱為禾穀鐮刀菌)人工感染。藉由確定受赤黴病影響(小穗變白)之小麥頭部百分比來量測疾病嚴重程度。疾病控制百分比基於考慮患病、未經處理之對照植物為100%。資料展示於以下表XV中。 For wheat scab, a single treatment is applied while the crop is flowering. After three days of treatment, the plants were artificially infected with the pathogen of the scab, Zea mays (also known as Fusarium graminearum). Disease severity was measured by determining the percentage of wheat head affected by scab (white spikes). The percentage of disease control was based on 100% of diseased, untreated control plants. The data is shown in Table XV below.

對於大豆銹病，進行6次處理施用。初始施用在生長R1階段輸送。此試驗具有天然感染。疾病嚴重程度藉由如白黴試驗中評估莖幹來量測。每個小塊土地樹冠六個2呎區段評估為子樣品且嚴重程度藉由自整個樹冠評估展示鏽症狀之葉百分比來確定。疾病控制百分比基於考慮患病、未經處理之對照植物為100%。資料展示於以下表XV中。 For soybean rust, 6 treatments were applied. Initial application is delivered during the growth R1 phase. This test has a natural infection. The severity of the disease is measured by assessing the stem as in the white mold test. The six 2-inch segments of each small land canopy were evaluated as subsamples and the severity was determined by assessing the percentage of leaves showing rust symptoms from the entire canopy. The percentage of disease control was based on 100% of diseased, untreated control plants. The data is shown in Table XV below.

對於番茄，進行2次處理施用。藉由在樹冠進行觀察且評估受影響葉之百分比來量測疾病嚴重程度。疾病控制百分比基於考慮患病、未經處理之對照植物為100%。資料展示於以下表XV中。 For tomato, 2 treatments were applied. Disease severity was measured by observing in the canopy and assessing the percentage of affected leaves. The percentage of disease control was based on 100% of diseased, untreated control plants. The data is shown in Table XV below.

如藉由未經處理之對照%所量測，RTI472比SERENADE OPTIMUM更好地控制小麥赤黴病。小麥赤黴病在未經處理之對照中具有85%之重度嚴重程度/壓力。如藉由未經處理之對照%所量測，RTI472比SERENADE OPTIMUM更好地控制大豆銹病。大豆銹病試驗在未經處理之對照中具有44%之中度嚴重程度/壓力。在包括小麥中施用一次、番茄中施用兩次及大豆中施用六次的處理施用方案中未指出對RTI472之陰性作物反應。 RTI 472 better controls wheat scab than SERENADE OPTIMUM, as measured by untreated control %. Wheat scab has 85% severity severity/pressure in untreated controls. RTI 472 better controls soybean rust than SERENADE OPTIMUM as measured by untreated control %. The soybean rust test had a moderate severity/pressure of 44% in the untreated control. Negative crop responses to RTI 472 were not indicated in the treatment regimen including one application in wheat, two in tomato, and six in soybean.

實例11Example 11 在喬治亞州蕃茄田間試驗中解澱粉芽孢桿菌RTI472對番茄細菌性斑病(黃單孢菌屬)之拮抗作用Antagonistic effect of Bacillus amyloliquefaciens RTI472 on tomato bacterial spot disease (Homomonospora) in a tomato field trial in Georgia

在番茄田間試驗中進行研究以確定解澱粉芽孢桿菌RTI472菌株預防及/或減輕植物病原體番茄細菌性斑病(黃單孢菌屬)作用的能力。 Studies were conducted in a tomato field trial to determine the ability of B. amyloliquefaciens RTI 472 strain to prevent and/or alleviate the effects of the plant pathogen tomato bacterial spot disease (Homomonospora).

對作物總共進行4次施用，其中施用之間的時間間隔為5至7天。在將生物活性成分與化學活性成分之施用組合的方案4及5之情況下，第一次及第三次施用用生物活性成分進行，而第二次及第四次施用用化學活性成分進行。 A total of 4 applications were applied to the crop with a time interval between administrations of 5 to 7 days. In the case of Schemes 4 and 5 in which the bioactive component is combined with the administration of the chemically active ingredient, the first and third administrations are carried out with the biologically active ingredient, and the second and fourth administrations are carried out with the chemically active ingredient.

調配物：Formulation:

SERENADE OPTIMUM以1400g/ha之施用率，對應於1.8×10+13CFU/ha施用。 SERENADE OPTIMUM an application rate of 1400g / ha of, corresponding to 1.8 × 10 +13 CFU / ha administration.

解澱粉芽孢桿菌RTI472孢子在廢醱酵液(SFB)中且添加酵母提取物，且施用率對應於與基於1400g/ha施用率，針對SERENADE OPTIMUM所推薦相同之菌落形成單位/公頃，且酵母提取物在約0.01%至0.2%範圍內。 Bacillus amyloliquefaciens RTI472 spores in spent broth (SFB) and added yeast extract, and the application rate corresponds to the same colony forming unit/ha as recommended for SERENADE OPTIMUM based on 1400 g/ha application rate, and yeast extraction The material is in the range of about 0.01% to 0.2%.

TACTIC(LOVELAND PRODUCTS,INC)以0.1875%v/v之濃度施用。 TACTIC (LOVELAND PRODUCTS, INC) was applied at a concentration of 0.1875% v/v.

KOCIDE 3000(DUPONT USA)以1850g a.i./ha之施用率(氫氧化銅殺真菌劑)施用。 KOCIDE 3000 (DUPONT USA) was applied at an application rate of 1850 g a.i./ha (copper hydroxide fungicide).

BRAVO WEATHER STIK(SYNGENTA CROP PROTECTION,INC)以2240g a.i./ha之施用率施用(四氯異苯腈)。 BRAVO WEATHER STIK (SYNGENTA CROP PROTECTION, INC) was applied at an application rate of 2240 g a.i./ha (tetrachloroisophthalonitrile).

實驗設計如下：未經處理之對照、RTI472+TACTIC、SERENADE OPTIMUM+TACTIC、RTI472+KOCIDE 3000+TACTIC、SERENADE OPTIMUM+KOCIDE 3000+TACTIC及BRAVO WEATHER STIK+KOCIDE 3000+TACTIC。 The experimental design is as follows: untreated control, RTI472+TACTIC, SERENADE OPTIMUM+TACTIC, RTI472+KOCIDE 3000+TACTIC, SERENADE OPTIMUM+KOCIDE 3000+TACTIC and BRAVO WEATHER STIK+KOCIDE 3000+TACTIC.

處理施用方法：Treatment application method:

將四次分開處理施用輸送至作物，其中各施用之間的時間間隔為5至7。施用噴霧器設定為輸送40加侖/英畝。個別小塊土地使用具有錐形噴嘴之CO2背包式噴霧器以3mph之地速噴灑且各噴嘴相隔12吋。輸送化學品之載劑為混合在2.5公升瓶中之水。 Four separate treatment applications were delivered to the crop with a time interval between 5 and 7 for each application. The sprayer was set to deliver 40 gallons per acre. Individual plots were sprayed at 3 mph using a CO 2 backpack sprayer with a conical nozzle and each nozzle was 12 相 apart. The carrier that transports the chemical is water mixed in a 2.5 liter bottle.

疾病嚴重程度藉由評估樹冠來量測。疾病嚴重程度平均%在各處理之植物中間評估。疾病控制百分比基於考慮患病、未經處理之對照植物為100%。資料展示於以下表XVI中。處理包括：未經處理之對照、RTI472+TACTIC、SERENADE OPTIMUM+TACTIC、RTI472+ KOCIDE 3000+TACTIC、SERENADE OPTIMUM+KOCIDE 3000+TACTIC及BRAVO WEATHER STIK+KOCIDE 3000+TACTIC。觀測到解澱粉芽孢桿菌RTI472作為單獨或在與KOCIDE一起之方案中對蕃茄上番茄細菌性斑病(黃單孢菌屬)之控制最佳，且勝過基於使用BRAVO WEATHER STIK(四氯異苯腈；SYNGENTA)與KOCIDE 3000組合之方案。 The severity of the disease was measured by assessing the canopy. The average % of disease severity was assessed in the middle of each treated plant. The percentage of disease control was based on 100% of diseased, untreated control plants. The data is shown in Table XVI below. Treatment includes: untreated control, RTI472+TACTIC, SERENADE OPTIMUM+TACTIC, RTI472+ KOCIDE 3000+TACTIC, SERENADE OPTIMUM+KOCIDE 3000+TACTIC and BRAVO WEATHER STIK+KOCIDE 3000+TACTIC. It was observed that B. amyloliquefaciens RTI472 was the best control of tomato bacterial spot disease (Homomonospora) on tomato alone or in combination with KOCIDE, and was superior to the use of BRAVO WEATHER STIK (tetrachloroisobenzene). Nitrile; SYNGENTA) combined with KOCIDE 3000.

實例12Example 12 用解澱粉芽孢桿菌RTI472分離株塗佈玉米種子之作用Effect of coating corn seed with Bacillus amyloliquefaciens RTI472 isolate

進行實驗以確定用除典型化學對照外的解澱粉芽孢桿菌RTI472菌株之孢子塗料玉米種子之作用。量測對達至植物萌芽之時間、植株密度、植物活力及籽粒產量之作用，求威斯康星州之三個田間試驗之平均值。如下所述進行實驗。 Experiments were performed to determine the effect of spore coating corn seed with a strain of Bacillus amyloliquefaciens RTI472 other than a typical chemical control. The effects of the time on plant germination, plant density, plant vigor and grain yield were measured and the average of three field trials in Wisconsin was obtained. The experiment was carried out as described below.

調配物：Formulation:

解澱粉芽孢桿菌RTI472孢子於水中之濃縮物(1.0×10+10cfu/ml)以每個種子1.0×10+5cfu之量施用。 A concentrate of Bacillus amyloliquefaciens RTI472 spores in water (1.0 x 10 + 10 cfu/ml) was applied in an amount of 1.0 x 10 + 5 cfu per seed.

MAXIM(SYNGENTA CROP PROTECTION,INC)以0.0064mg AI/核(護汰寧)施用於種子。 MAXIM (SYNGENTA CROP PROTECTION, INC) was applied to the seeds at 0.0064 mg AI/nuclear (Ning Ning).

滅達樂以0.005mg AI/核施用於種子。 Verbrail was applied to the seeds at 0.005 mg AI/nucleus.

PONCHO 250(BAYER CROP SCIENCE)以0.25mg AI/核(可尼丁)施用於種子。 PONCHO 250 (BAYER CROP SCIENCE) was applied to the seeds at 0.25 mg AI/nuclear (konidine).

處理施用方法：Treatment application method:

藉由將玉米種子與含有解澱粉芽孢桿菌RTI472孢子及化學對照MAXIM+滅達樂+PONCHO 250之溶液混合來進行種子處理，該溶液產生每顆種子1×105cfu之平均值及如以上詳述的標記指示濃度之化學活性成分。用未經處理之種子及經單獨化學對照處理之種子作為對照進行實驗。未經處理之種子及經處理之各玉米種子種植在威斯康星州三個分開田間試驗中且藉由達至植物萌芽之時間長度、植株密度、植物活力及籽粒產量(蒲式耳/英畝)分析。使用來自三個田間試驗之資料的平均值，與未經處理之種子相比，添加化學對照使得達至植物萌芽之時間、植株密度、植物活力及籽粒產量各者統計學上顯著增加。與經單獨化學對照處理之種子相比，種子處理劑中包括解澱粉芽孢桿菌RTI472對達至植物萌芽之時間、植株密度或植物活力無統計學上顯著之作用，但引起顆粒6蒲式耳/英畝之增加(231至237蒲式耳/英畝)，表示籽粒產量增加2.6%。 Seed treatment was carried out by mixing corn seeds with a solution containing Bacillus amyloliquefaciens RTI 472 spores and a chemical control MAXIM + statin + PONCHO 250, which produced an average of 1 x 10 5 cfu per seed and as detailed above The label indicates the concentration of the chemically active ingredient. Experiments were performed using untreated seeds and seeds treated with separate chemical controls as controls. Untreated seeds and treated corn seeds were planted in three separate field trials in Wisconsin and analyzed by time to plant germination, plant density, plant vigor and grain yield (bushel/acre). Using the average of the data from the three field trials, the addition of a chemical control resulted in a statistically significant increase in the time to plant germination, plant density, plant vigor, and grain yield, as compared to untreated seeds. Compared with the seeds treated with the chemical control alone, the B. amyloliquefaciens RTI472 included in the seed treatment agent had no statistically significant effect on the time to plant germination, plant density or plant vigor, but caused the particles to be 6 bushels per acre. An increase (231 to 237 bushels per acre) indicates a 2.6% increase in grain production.

如上所述進行相關試驗，其中例外為分開用病原體絲核菌及禾穀鐮刀菌攻擊玉米植物。藉由目視檢查依1至5之量表將疾病嚴重程度評級。與經單獨化學對照處理之種子相比，用解澱粉芽孢桿菌RTI472處理種子使得絲核菌及天然存在之疾病的疾病嚴重程度統計學上顯著降低。 The relevant tests were carried out as described above, with the exception of attacking corn plants with the pathogen Rhizoctonia and Fusarium graminearum separately. The severity of the disease was rated by visual inspection on a scale of 1 to 5. Treatment of the seeds with B. amyloliquefaciens RTI 472 resulted in a statistically significant reduction in the severity of the disease of Rhizoctonia and naturally occurring diseases compared to seeds treated with a separate chemical control.

實例13Example 13 意大利(Italy)及西班牙(Spain)田間試驗中解澱粉芽孢桿菌RTI472對草莓中褐灰黴(灰葡萄孢菌)之拮抗作用Antagonistic effects of Bacillus amyloliquefaciens RTI472 on the gray mold of Botrytis cinerea in Italy (Italy) and Spain (Spain)

在草莓田間試驗中進行研究以確定解澱粉芽孢桿菌RTI472菌株預防及/或減輕植物病原體褐灰黴(灰葡萄孢菌)作用的能力。 Studies were conducted in a strawberry field trial to determine the ability of B. amyloliquefaciens RTI 472 strain to prevent and/or alleviate the effects of the plant pathogen, Botrytis cinerea (B. cinerea).

對作物總共進行4次施用，其中施用之間的時間間隔為7天。 A total of 4 applications were applied to the crop with a time interval between administrations of 7 days.

調配物：Formulation:

SERENADE MAX以4000g/ha之施用率，對應於2.0×10+14CFU/ha之枯草芽孢桿菌菌株QST713施用。 SERENADE MAX was applied at an application rate of 4000 g/ha corresponding to 2.0 x 10 + 14 CFU/ha of Bacillus subtilis strain QST713.

解澱粉芽孢桿菌RTI472孢子廢醱酵液(SFB)中且添加酵母提取物且施用率為2.0×10+13CFU/ha，其中酵母提取物在約0.01%至0.2%範圍內。另外，一種非離子型有機聚矽氧界面活性劑SILWET L77(HELENA CHEMICAL)以每100公升噴霧溶液0.15公升之施用率添加。 Spores of Bacillus amyloliquefaciens RTI472 Po spent fermentation broth (SFB) and the yeast extract and the application rate of 2.0 × 10 +13 CFU / ha, wherein the yeast extract in the range of about 0.01% to 0.2%. Further, a nonionic organopolyfluorene surfactant SILWET L77 (HELENA CHEMICAL) was added at an application rate of 0.15 liter per 100 liters of the spray solution.

用SWITCH(嘧菌環胺375g/kg加上護汰寧250g/kg；SYNGENTA CROP PROTECTION,INC)以0.8kg/ha之施用率進行作物之頭兩次施用，接著以1.8kg/ha之施用率兩次施用SIGNUM(白可列267g/kg加上百克敏67g/kg；BAYER CROP SCIENCE,INC)。此在本文中稱為「FARMER方案」。 The first two applications of the crop were carried out with SWITCH (cytosine 375 g/kg plus shining 250 g/kg; SYNGENTA CROP PROTECTION, INC) at an application rate of 0.8 kg/ha, followed by an application rate of 1.8 kg/ha. SIGNIUM was administered twice (white 267 g/kg plus 100 g/67 g/kg; BAYER CROP SCIENCE, INC). This is referred to herein as the "FARMER program."

實驗設計如下：未經處理之對照(UTC)、FARMER方案、RTI472+SILWET L77及SERENADE MAX。 The experimental design is as follows: untreated control (UTC), FARMER protocol, RTI472+SILWET L77 and SERENADE MAX.

處理施用方法：Treatment application method:

進行四次獨立田間試驗，各具有4個複本，且試驗結果組合且作為平均值結果呈現。將四次分開處理施用輸送至作物，其中各施用之間的時間間隔為7天。在開始處理前之三天，所有小塊土地，包括未經處理之對照，均經SWITCH處理以遏制初始疾病發展。施用噴霧器設定為輸送53至107加侖/英畝，視施用時作物密度而定。個別小塊土地使用具有錐形噴嘴之CO2背包式噴霧器以0.56mph(0.25m/s或0.9km/h)之地速噴灑且各噴嘴相隔2吋(5.5cm)。 Four independent field trials were performed, each with 4 replicates, and the test results were combined and presented as average results. Four separate treatment applications were delivered to the crop with a time interval between each application of 7 days. Three days before the start of treatment, all small plots, including untreated controls, were treated with SWITCH to contain the initial disease progression. The application sprayer is set to deliver between 53 and 107 gallons per acre depending on the crop density at the time of application. Individual plots were sprayed at a ground speed of 0.56 mph (0.25 m/s or 0.9 km/h) using a CO 2 backpack sprayer with a conical nozzle and each nozzle was 2 吋 (5.5 cm) apart.

將所有草莓收穫，計數，稱重，且分成可銷售或患病。疾病發病率(受褐灰黴影響之果實%)藉由在6個分開日期評估收穫時各處理之果實來量測，且表示為「疾病壓力曲線下面積」(AUDPC)。相對於未經處理之對照的疾病發病率及產量增加%展示於以下表XVIII中。 UTC之疾病發病率隨時間之變化展示在以下圖(圖13)，且展示在試驗期間之疾病壓力到達感染果實約20%至45%之最高疾病壓力。 All strawberries are harvested, counted, weighed, and divided into saleable or diseased. The incidence of disease (% of fruit affected by B. cinerea) was measured by assessing the fruits of each treatment at the time of harvest on six separate dates and expressed as "area under the disease pressure curve" (AUDPC). The disease incidence and % increase in yield relative to the untreated control are shown in Table XVIII below. The change in UTC disease incidence over time is shown in the following figure (Figure 13) and shows that the disease pressure during the trial reaches the highest disease stress of about 20% to 45% of the infected fruit.

以下表XVIII中之結果展示對所有三個處理，解澱粉芽孢桿菌RTI472、SERENADE MAX及FARMER方案，均觀測到草莓上褐灰黴(灰葡萄孢菌)比未經處理之對照改善之控制，其中用RTI472處理之產量增加數值上略微更高。 The results in Table XVIII below show that for all three treatments, Bacillus amyloliquefaciens RTI472, SERENADE MAX and FARMER protocols, the control of the improvement of the brown mold (B. cinerea) on the strawberry was observed compared to the untreated control. The yield increase with RTI472 treatment was slightly higher.

實例14Example 14 解澱粉芽孢桿菌RTI472對夏南瓜中白粉病之拮抗作用Antagonistic effect of Bacillus amyloliquefaciens RTI472 on powdery mildew in zucchini

在夏南瓜田間試驗中進行研究，以確定解澱粉芽孢桿菌RTI472菌株單獨或作為與FRACTURE之混合物，預防及/或減輕夏南瓜中由高氏白粉病菌(ERYSCI)所引起之白粉病之作用的能力。FRACTURE(CONSUMO EM VERDE(CEV),BIOTECNOLOGIA DAS PLANTAS S.A.,PORTUGAL)為一種基於植物提取物之調配物，其含有20%BLAD多肽作為活性成分。BLAD多肽為來自甜羽扇豆(白羽扇豆)之天然存在之種子儲存蛋白的片段，其藉由對真菌細胞壁造成損害且破壞內細胞膜而作用於易感真菌病原體。 Studies were conducted in a zucchini field trial to determine the ability of B. amyloliquefaciens RTI 472 strain alone or as a mixture with FRACTURE to prevent and/or alleviate the effects of powdery mildew caused by powdery mildew (TERSCI) in zucchini . FRACTURE (CONSUMO EM VERDE (CEV), BIOTECNOLOGIA DAS PLANTAS S.A., PORTUGAL) is a plant extract-based formulation containing 20% BLAD polypeptide as an active ingredient. The BLAD polypeptide is a fragment of a naturally occurring seed storage protein from sweet lupin (white lupin) that acts on susceptible fungal pathogens by causing damage to the fungal cell wall and destroying the inner cell membrane.

除非另外說明，否則整體上，使用如以下表XIX中所指示之施用率，各產品進行6次施用，其中施用之間的時間間隔為5至7天。預期 疾病即將出現前進行第一次施用之時間安排。在RTI472(**施用率為1.8×10+13CFU/ha)之情況下，施用率對應於與基於1400g/ha施用率，針對SERENADE OPTIMUM所推薦相同之菌落形成單位/公頃。 Unless otherwise stated, overall, each product was applied 6 times using an application rate as indicated in Table XIX below, with a time interval between administrations of 5 to 7 days. The timing of the first application before the onset of the disease is expected. In the case of RTI 472 (** application rate 1.8×10 +13 CFU/ha), the application rate corresponds to the same colony forming unit/ha as recommended for SERENADE OPTIMUM based on the application rate of 1400 g/ha.

調配物：Formulation:

SERENADE OPTIMUM(BAYER CROP SCIENCE,INC)以1400g/ha，對應於1.8×10+13CFU/ha之施用率施用。 SERENADE OPTIMUM (BAYER CROP SCIENCE, INC ) to 1400g / ha, corresponding to 1.8 × 10 +13 CFU / ha, of administration.

FRACTURE(CONSUMO EM VERDE(CEV),BIOTECNOLOGIA DAS PLANTAS S.A.,PORTUGAL)以672g a.i./ha施用(BLAD)。 FRACTURE (CONSUMO EM VERDE (CEV), BIOTECNOLOGIA DAS PLANTAS S.A., PORTUGAL) was administered at 672 g a.i./ha (BLAD).

解澱粉芽孢桿菌RTI472孢子加上廢醱酵液(SFB)且添加0.15%酵母提取物、0.2%蔗糖、0.02%MgSO4及0.002%CaCl2，且施用率對應於與基於1400g/ha施用率，針對SERENADE OPTIMUM所推薦相同之菌落形成單位/公頃(CFU/ha)。 Bacillus amyloliquefaciens RTI472 spore plus waste mash (SFB) and 0.15% yeast extract, 0.2% sucrose, 0.02% MgSO 4 and 0.002% CaCl 2 were added , and the application rate corresponds to an application rate based on 1400 g/ha. The same colony forming units/ha (CFU/ha) are recommended for SERENADE OPTIMUM.

BRAVO WEATHER STIK(SYNGENTA CROP PROTECTION,INC)產品以1680g a.i./ha之施用率施用(四氯異苯腈)。 The BRAVO WEATHER STIK (SYNGENTA CROP PROTECTION, INC) product was applied at an application rate of 1680 g a.i./ha (tetrachloroisophthalonitrile).

LUNA EXPERIENCE(BAYER CROP SCIENCE,INC)以500g a.i./ha之施用率施用(氟吡菌醯胺加上得克利殺真菌劑)。 LUNA EXPERIENCE (BAYER CROP SCIENCE, INC) was applied at an application rate of 500 g a.i./ha (fluopyram and plus kle fungicide).

TACTIC(LOVELAND PRODUCTS,INC)以0.1875%v/v之濃度施用。 TACTIC (LOVELAND PRODUCTS, INC) was applied at a concentration of 0.1875% v/v.

實驗設計如下：未經處理之對照、FRACTURE、SERENADE OPTIMUM+TACTIC、BRAVO WEATHER STIK+TACTIC、LUNA EXPERIENCE+TACTIC、RTI472+TACTIC、RTI472+FRACTURE +TACTIC。 The experimental design is as follows: untreated control, FRACTURE, SERENADE OPTIMUM+TACTIC, BRAVO WEATHER STIK+TACTIC, LUNA EXPERIENCE+TACTIC, RTI472+TACTIC, RTI472+FRACTURE +TACTIC.

處理施用方法：除非另外說明，否則所有施用均進行6次，其中施用之間的時間間隔為5至7天。施用噴霧器設定為輸送30加侖/英畝。個別小塊土地使用具有平扇形噴嘴(8004類型)之CO2背包式噴霧器以4mph之地速噴灑且各噴嘴相隔18吋。輸送化學品之載劑為混合 在2公升瓶中之水。對於RTI472加上FRACTURE之施用，兩種產品進行桶混且以與個別產品相同之濃度施用。 Treatment Method of Administration: All applications were performed 6 times, unless otherwise stated, with a time interval between administrations of 5 to 7 days. The sprayer was set to deliver 30 gallons per acre. Individual plots were sprayed at a ground speed of 4 mph using a CO 2 backpack sprayer with a flat fan nozzle (type 8004) and each nozzle was 18 inches apart. The carrier that transports the chemical is water mixed in a 2 liter bottle. For the application of RTI 472 plus FRACTURE, the two products were tank mixed and applied at the same concentration as the individual products.

疾病評分：夏南瓜上白粉病依頂葉之程度評估。表示為「疾病壓力曲線下面積」(AUDPC)之疾病嚴重程度作為上葉表面上白粉病之嚴重程度的函數(受影響葉面積%)確定。未經處理之植物確定具有100%疾病發病率及62.8%疾病嚴重程度。 Disease score: The severity of powdery mildew on zucchini is assessed by the degree of parietal lobe. The severity of the disease expressed as "area under the disease stress curve" (AUDPC) was determined as a function of the severity of powdery mildew on the upper leaf surface (% of affected leaf area). Untreated plants were determined to have a 100% disease incidence and 62.8% disease severity.

實驗結果展示於以下表XIX中。FRACTURE提供疾病嚴重程度大約15%減少，而SERENADE OPTIMUM及RTI472得到疾病嚴重程度大約30%減少。觀測到BRAVO WEATHER STIK(45%)及LUNA EXPERIENCE(94%)之疾病嚴重程度減少最顯著。有趣地，當RTI472及FRACTURE一起施用時，觀測到疾病控制之累積作用，引起疾病嚴重程度47%之減少。此與BRAVO WEATHER STIK之疾病嚴重程度減少(45%)相當。因此，基於解澱粉芽孢桿菌RTI472及FRACTURE之產品組合可用作控制葫蘆上白粉病之方案中四氯異苯腈殺真菌劑的替代。 The experimental results are shown in Table XIX below. FRACTURE provides approximately a 15% reduction in disease severity, while SERENADE OPTIMUM and RTI472 have a reduction in disease severity of approximately 30%. The reduction in disease severity was most pronounced in BRAVO WEATHER STIK (45%) and LUNA EXPERIENCE (94%). Interestingly, when RTI 472 and FRACTURE were administered together, the cumulative effect of disease control was observed, causing a 47% reduction in disease severity. This is comparable to the reduced severity of disease (45%) in BRAVO WEATHER STIK. Therefore, a product combination based on Bacillus amyloliquefaciens RTI472 and FRACTURE can be used as an alternative to the tetrachloroisophthalonitrile fungicide in the scheme for controlling powdery mildew on the gourd.

* 施用率，g a.i./ha * application rate, g a.i./ha

** 施用率，cfu/ha ** Application rate, cfu/ha

+ 指示施用之時間安排(具有字母/日期) + indicates the timing of the application (with letters/dates)

N.A. 不適用 N.A. Not applicable

實例15Example 15 鑑別由解澱粉芽孢桿菌RTI472分離株產生之新代謝物Identification of new metabolites produced by Bacillus amyloliquefaciens RTI472 isolates

先前已報導五種類別豐原素型代謝物及去羥基豐原素型代謝物藉由包括解澱粉芽孢桿菌之微生物物種產生(參見例如Li,Xing-Yu等人，2013,J.Microbiol.Biotechnol.23(3),313-321；PecciY等人，2010, Mass Spectrom.,45(7)：772-77)。此等代謝物環狀脂肽為亦含有脂肪酸基之環狀肽分子。五種類別豐原素及去羥基豐原素型代謝物稱為A、B、C、D及S。五種類別各者之此等代謝物之主鏈結構以及特定胺基酸序列展示在圖13中。 It has previously been reported that five classes of abundane-type metabolites and dehydroxy-hydroxytoxin-type metabolites are produced by microbial species including Bacillus amyloliquefaciens (see, for example, Li, Xing-Yu et al., 2013, J. Microbiol. Biotechnol. 23 (3), 313-321; PecciY et al., 2010, Mass Spectrom., 45(7): 772-77). These metabolite cyclic lipopeptides are cyclic peptide molecules which also contain fatty acid groups. The five classes of abundance and dehydroxyl-producing metabolites are called A, B, C, D and S. The backbone structure of these metabolites and the specific amino acid sequence for each of the five classes are shown in Figure 13.

藉由解澱粉芽孢桿菌RTI472產生之豐原素型及去羥基豐原素型代謝物使用UHPLC-TOF MS分析。在869培養基中30℃下生長3與6天之後藉由RTI472菌株產生之豐原素型代謝物之分子量與針對豐原素型及去羥基豐原素型代謝物所預期之理論分子量相比。另外，為確定藉由RTI472菌株產生之各種豐原素型代謝物之胺基酸組成，對先前經由UHPLC-TOF MS鑑別之豐原素型代謝物各者使用LC-MS-MS進行肽測序。以此方式，確定解澱粉芽孢桿菌RTI472產生豐原素A、豐原素B、豐原素C、豐原素D及豐原素S及去羥基豐原素A、去羥基豐原素B、去羥基豐原素C、去羥基豐原素D及去羥基豐原素S。意外地，除此等已知之化合物之外，確定RTI472菌株亦產生若干類別之先前未鑑別之此等化合物衍生物。 Ubiquitin-type and dehydroxy-hydroxytoxin-type metabolites produced by B. amyloliquesii RTI472 were analyzed using UHPLC-TOF MS. The molecular weight of the abundane-type metabolite produced by the RTI 472 strain after growth for 3 and 6 days at 30 ° C in 869 medium was compared to the theoretical molecular weight expected for the abundance of the abundance and the dehydroxyl-type metabolite. In addition, to determine the amino acid composition of various abundane-type metabolites produced by the RTI 472 strain, peptide sequencing was performed using LC-MS-MS for each of the abundane-type metabolites previously identified by UHPLC-TOF MS. In this way, it was determined that B. amyloliquefaciens RTI472 produced abundance of A, abundance of B, abundance of C, abundance of D and abundance of S, and a dehydroxyl-producin A, a dehydroxyl-producin B, a dehydroxy-hydroxytoxin C, Hydroxy-rich protamine D and dehydroxy-hydroxytoxin S. Surprisingly, in addition to such known compounds, it was determined that the RTI 472 strain also produced several classes of previously unidentified such compound derivatives.

舉例而言，確定解澱粉芽孢桿菌RTI472菌株產生豐原素樣及去羥基豐原素樣化合物，其中環狀肽鏈之位置8(圖14中稱為X3)之L-異 白胺酸經L-甲硫胺酸置換。新類別豐原素及去羥基豐原素在本文中稱為MA、MB及MC，係指A類、B類及C類衍生物，其中圖14中X3處之L-異白胺酸經L-甲硫胺酸置換。新鑑別之分子以粗體展示於圖14及以下表XX中。如表XX所述，在RTI472菌株之培養物中未觀測到去羥基豐原素MC化合物。 For example, it is determined that the B. amyloliquefaciens RTI472 strain produces a protosanoid-like and dehydroxy-norformin-like compound in which the L-isoleucine acid at position 8 (referred to as X 3 in FIG. 14) of the cyclic peptide chain is L- Methionine replacement. The new classes of protosan and dehydroxylectin are referred to herein as MA, MB and MC, and refer to class A, B and C derivatives, wherein L-isoleucine at X 3 in Figure 14 is L- Methionine replacement. The newly identified molecules are shown in bold in Figure 14 and in Table XX below. As shown in Table XX, no dehydroxylectin MC compound was observed in the culture of the RTI472 strain.

進一步確定解澱粉芽孢桿菌RTI472菌株產生其他類別之先前未鑑別豐原素(及假定去羥基豐原素)代謝物。在此類別中，豐原素B之L-異白胺酸(圖13中之位置X3)經L-高半胱胺酸(Hcy)置換。此先前未鑑別之代謝物在本文中稱作豐原素H。 It was further determined that the B. amyloliquefaciens RTI 472 strain produced other classes of previously unidentified abundane (and putative dehydroxylectin) metabolites. In this category, L- fengycin B is isoleucine (FIG. 13 in the position X 3) via L- homocysteine (of Hcy) replacement. This previously unidentified metabolite is referred to herein as a protocin H.

進一步確定RTI472菌株產生其他類別先前未鑑別之豐原素及去羥基豐原素代謝物。在此類別中，環狀肽主鏈結構之位置4(圖14中之位置X1)的胺基酸經L-異白胺酸置換。此等先前未鑑別之代謝物本文中稱為豐原素I及去羥基豐原素I且以粗體展示在圖14及表XX中。 It was further determined that the RTI 472 strain produced other classes of previously unidentified abundance and dehydroxylectin metabolites. In this category, the position of the main chain structure of the cyclic peptide 4 (FIG. 14 in the position X 1) is replaced by the amino acid L- isoleucine. These previously unidentified metabolites are referred to herein as protamine I and dehydroxylectin I and are shown in bold in Figure 14 and Table XX.

下表XX中提供先前報導之豐原素型及去羥基豐原素型脂肽及新鑑別之代謝物的胺基酸序列的概述。 A summary of the amino acid sequences of the previously reported abundance forms and dehydroxyl-preparative lipopeptides and newly identified metabolites is provided in Table XX below.

實例16 Example 16 RTI472之分離脂肽代謝物的抗微生物活性Antimicrobial activity of isolated lipopeptide metabolites of RTI472

來自解澱粉芽孢桿菌菌株RTI472之拮抗脂肽自RTI472廢醱酵液分離且展示其保留其活性。 The antagonist lipopeptide from B. amyloliquefaciens strain RTI472 was isolated from RTI 472 waste mash and was shown to retain its activity.

在此實驗中，根據Smyth,TJP等人，2010，「Isolation and Analysis of Lipopeptides and High Molecular Weight Biosurfactants.」：Handbook of Hydrocarbon and Lipid Microbiology,K.N.Timmis(編輯).第3687-3704頁中描述之程序，解澱粉芽孢桿菌培養物上清液酸化至pH 2。藉由UHPLC-TOF MS分析脂肽之回收，且測試其針對灰葡萄孢菌及禾穀鐮刀菌之拮抗活性。 In this experiment, the procedure described in Smyth, TJP et al., 2010, "Isolation and Analysis of Lipopeptides and High Molecular Weight Biosurfactants.": Handbook of Hydrocarbon and Lipid Microbiology, KNTimmis (eds.), pp. 3687-3704. The B. amyloliquefacium culture supernatant was acidified to pH 2. The recovery of lipopeptides was analyzed by UHPLC-TOF MS and tested for antagonistic activity against Botrytis cinerea and Fusarium graminearum.

RTI472在M2孢子形成培養基中在30℃下培養六天，且廢醱酵液 (472-SFB)在18,514g下離心20分鐘以移除孢子。上清液隨後藉由添加濃HCl酸化至pH 2.0，且在4℃下沈澱隔夜。隨後樣品在18,514g下離心20分鐘以獲得固體粗脂肽。球粒凍乾隔夜，溶解於原始體積之M2培養基中，且藉由LCMS分析。提取伊枯草菌素(C14、C15、C16)、表面活性素(C12、C13、C14、C15、C16、C17)及豐原素(A、B、C、D、S)之質量，整合且計算總和，以比較來自各樣品之脂肽的相對豐度。圖15為展示在酸沈澱之後自RTI472廢醱酵液(SFB)回收之脂肽的百分比之圖。術語「472-AP-球粒」及「472-AP-上清液」分別指在離心之SFB進行酸沈澱之後獲得的再懸浮球粒及上清液。圖15中之圖的結果展示脂肽總量之83%藉由酸沈澱回收。對於伊枯草菌素，沈澱65%，而經由酸沈澱方法，26%伊枯草菌素未回收。表面活性素及豐原素使用酸沈澱100%回收。 RTI472 was cultured in M2 sporulation medium at 30 ° C for six days, and waste broth (472-SFB) was centrifuged at 18,514 g for 20 minutes to remove spores. The supernatant was then acidified to pH 2.0 by the addition of concentrated HCl and precipitated overnight at 4 °C. The sample was then centrifuged at 18,514 g for 20 minutes to obtain a solid crude lipopeptide. The pellets were lyophilized overnight, dissolved in the original volume of M2 medium and analyzed by LCMS. Extract the quality of italin (C14, C15, C16), surfactant (C12, C13, C14, C15, C16, C17) and agrosperm (A, B, C, D, S), integrate and calculate the sum To compare the relative abundance of lipopeptides from each sample. Figure 15 is a graph showing the percentage of lipopeptides recovered from RTI 472 waste mash (SFB) after acid precipitation. The terms "472-AP-spherule" and "472-AP-supernatant" refer to the resuspended pellets and supernatant obtained after acid precipitation of SFB in centrifugation, respectively. The results in the graph of Figure 15 show that 83% of the total lipopeptide was recovered by acid precipitation. For isalectin, 65% was precipitated, while 26% of the avermectin was not recovered by the acid precipitation method. Surfactant and abundance are 100% recovered using acid precipitation.

為證實LCMS結果與拮抗活性相關，用藉由LCMS分析之相同樣品進行生物分析。對於該生物分析，20μl灰葡萄孢菌或禾穀鐮刀菌接種體在培養盤中間點樣，其中472-AP-球粒樣品在10μl、20μl及40μl等分試樣中點樣。對於灰葡萄孢菌及禾穀鐮刀菌盤，分別在30℃下培育5天或7天之後，檢查抗真菌活性且成像。結果展示在圖16A-16D中盤分析之影像中。圖16A及16B為點樣在具有以下之869瓊脂盤上之灰葡萄孢菌的影像：A)RTI472廢醱酵液(472-SFB)；及B)在酸沈澱加上離心之後獲得之再懸浮球粒物質(472-AP-球粒)。圖16C及16D為點樣在具有以下之869瓊脂盤上之禾穀鐮刀菌的影像：C)RTI472廢醱酵液(472-SFB)；及D)在酸沈澱加上離心之後獲得之再懸浮球粒物質(472-AP-球粒)。對於陽性對照，10μl RTI472細胞培養物點樣在各盤左側，緊挨真菌。結果展示酸沈澱之樣品(472-AP-球粒)與起始廢醱酵液具有類似程度之針對灰葡萄孢菌與禾穀鐮刀菌之拮抗活性。生物分析結果與圖15中LCMS資料良好相關。 To confirm that the LCMS results correlate with the antagonistic activity, bioassays were performed using the same samples analyzed by LCMS. For this bioassay, 20 [mu]l of Botrytis cinerea or Fusarium graminearum inoculum was spotted in the middle of the plates, where 472-AP-spherule samples were spotted in 10 [mu]l, 20 [mu]l and 40 [mu]l aliquots. For Botrytis cinerea and Fusarium graminearum, after 5 or 7 days of incubation at 30 ° C, respectively, antifungal activity was examined and imaged. The results are shown in the images of the disc analysis in Figures 16A-16D. Figures 16A and 16B are images of Botrytis cinerea spotted on a 869 agar plate with the following: A) RTI 472 waste mash (472-SFB); and B) resuspended after acid precipitation plus centrifugation Globular material (472-AP-pellet). Figures 16C and 16D are images of Fusarium graminearum spotted on a 869 agar plate with the following: C) RTI 472 waste broth (472-SFB); and D) resuspended after acid precipitation plus centrifugation Globular material (472-AP-pellet). For the positive control, 10 μl of RTI472 cell culture was spotted on the left side of each plate, next to the fungus. The results show that the acid precipitated sample (472-AP-spherule) has a similar degree of antagonistic activity against Botrytis cinerea and Fusarium graminearum as the starting waste mash. The results of the bioassay are well correlated with the LCMS data in Figure 15.

參考文獻 references

本說明書中提及之所有公開案、專利申請案、專利及其他參考文獻均以全文引用的方式併入本文中。 All publications, patent applications, patents, and other references mentioned in this specification are hereby incorporated by reference in their entirety.

雖然以上標的物已以說明及實例方式出於清楚瞭解之目的相當詳細地描述，但熟習此項技術者應瞭解某些改變及修改可在所附申請專利範圍之範疇內進行。 Although the above-described subject matter has been described in considerable detail for the purpose of clarity and understanding, it will be understood by those skilled in the art that certain changes and modifications can be made within the scope of the appended claims.

【生物材料寄存】【Biomaterial Storage】 國內寄存資訊【請依寄存機構、日期、號碼順序註記】 Domestic registration information [please note according to the registration authority, date, number order]

1.食品工業發展研究所生物資源保存及研究中心；105年04月26日；BCRC910729 1. Center for Biological Resource Conservation and Research, Institute of Food Industry Development; April 26, 105; BCRC910729

國外寄存資訊【請依寄存國家、機構、日期、號碼順序註記】 Foreign deposit information [please note according to the country, organization, date, number order]

1.美國；American Type Culture Collection(ATCC)；2014年04月17日；PTA-121166 1. United States; American Type Culture Collection (ATCC); April 17, 2014; PTA-121166

<110> 美商富曼西公司 <110> American Fumanxi Company

<120> 解澱粉芽孢桿菌RTI472組合物及其於使植物生長獲益及治療植物疾病之使 用方法 <120> Bacillus amyloliquefaciens RTI472 composition and its benefits in plant growth and treatment of plant diseases Method

<130> 61084-PCT <130> 61084-PCT

<160> 2 <160> 2

<170> PatentIn version 3.5 <170> PatentIn version 3.5

<210> 1 <210> 1

<211> 1554 <211> 1554

<212> DNA <212> DNA

<213> 解澱粉芽孢桿菌 <213> Bacillus amyloliquefaciens

<400> 1 <400> 1

<210> 2 <210> 2

<211> 3246 <211> 3246

<212> DNA <212> DNA

<213> 解澱粉芽孢桿菌 <213> Bacillus amyloliquefaciens

<400> 2 <400> 2

Claims (109)

一種組合物，該組合物包含以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物，用於施用於植物以達成以下之一者或兩者：使植物生長獲益或對易感病植物賦予保護以避免病原性感染。 A composition comprising a Bacillus amyloliquefaciens RTI472 deposited with ATCC No. PTA-121166 or a biologically pure culture thereof having mutants of all of its identifying characteristics for application to a plant to achieve one of the following or Both: Benefit from plant growth or protect against susceptible plants to avoid pathogenic infections. 如請求項1之組合物，其中該植物生長益處及/或該賦予之保護係藉由以下展現：經提高之幼苗活力、經改善之根發育、經改善之植物生長、經提高之植物健康、經增加之產量、經改善之外觀、對植物病原體之經提高之抗性、經減少之病原性感染或其組合。 The composition of claim 1, wherein the plant growth benefit and/or the conferred protection is manifested by improved seedling vigor, improved root development, improved plant growth, improved plant health, Increased yield, improved appearance, increased resistance to plant pathogens, reduced pathogenic infection, or a combination thereof. 如請求項1之組合物，其中該組合物呈液體、粉劑、乾燥可潤濕粉末、可撒佈顆粒或乾燥可潤濕顆粒形式。 The composition of claim 1 wherein the composition is in the form of a liquid, a powder, a dry wettable powder, a spreadable granule or a dry wettable granule. 如請求項1之組合物，其中該組合物呈液體形式，且該解澱粉芽孢桿菌RTI472係以約1.0×108CFU/ml至約1.0×1012CFU/ml之濃度存在。 The composition of claim 1, wherein the composition is in liquid form, and the B. amyloliquefaciens RTI 472 is present at a concentration of from about 1.0 x 10 8 CFU/ml to about 1.0 x 10 12 CFU/ml. 如請求項1之組合物，其中該組合物呈粉劑、乾燥可潤濕粉末、可撒佈顆粒或乾燥可潤濕顆粒形式，且該解澱粉芽孢桿菌RTI472係以約1.0×108CFU/g至約1.0×1012CFU/g之量存在。 The composition of claim 1, wherein the composition is in the form of a powder, a dry wettable powder, a spreadable granule or a dry wettable granule, and the Bacillus amyloliquefaciens RTI 472 is about 1.0 x 10 8 CFU/g. It is present in an amount of about 1.0 x 10 12 CFU/g. 如請求項1之組合物，其中該組合物呈油性分散液形式，且該解澱粉芽孢桿菌RTI472係以約1.0×108CFU/ml至約1.0×1012CFU/ml之濃度存在。 The composition of claim 1, wherein the composition is in the form of an oily dispersion, and the B. amyloliquefaciens RTI472 is present at a concentration of from about 1.0 x 10 8 CFU/ml to about 1.0 x 10 12 CFU/ml. 如請求項1之組合物，其中該解澱粉芽孢桿菌RTI472呈孢子或營養細胞形式。 The composition of claim 1, wherein the B. amyloliquefaciens RTI 472 is in the form of spores or vegetative cells. 如請求項1之組合物，其進一步包含存在適合於使植物生長獲益及/或對易感病植物賦予保護以避免病原性感染之量之以下之一 或組合：微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑、除草劑、植物提取物、植物生長調節劑或肥料。 The composition of claim 1 further comprising one of the following amounts present in an amount suitable for benefiting plant growth and/or conferring protection to susceptible plants to avoid pathogenic infections Or combination: microbial, biological or chemical insecticides, fungicides, nematicides, fungicides, herbicides, plant extracts, plant growth regulators or fertilizers. 如請求項1之組合物，其進一步包含以下之一或組合：載劑、界面活性劑、分散劑或酵母提取物。 The composition of claim 1, which further comprises one or a combination of: a carrier, a surfactant, a dispersant, or a yeast extract. 如請求項1之組合物，其中該組合物呈種植基質形式。 The composition of claim 1 wherein the composition is in the form of a planting matrix. 如請求項10之組合物，其中該種植基質呈盆栽土形式。 The composition of claim 10, wherein the planting substrate is in the form of potting soil. 一種經以組合物塗佈之植物種子，該組合物包含：以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472，或其具有其所有鑑別特徵之突變體的生物純培養物的孢子，以適合使植物生長獲益及/或對易感病植物賦予保護以避免病原性感染之量存在。 A plant seed coated with a composition comprising: a spore of a biologically pure culture of a Bacillus amyloliquefaciens RTI472 deposited with ATCC No. PTA-121166, or a mutant thereof having all of its identifying characteristics, It is suitable for the benefit of plant growth and/or the protection of susceptible plants to avoid the presence of pathogenic infections. 如請求項12之植物種子，其中該植物生長益處及/或該賦予之保護係藉由以下展現：經提高之幼苗活力、經改善之根發育、經改善之植物生長、經提高之植物健康、經增加之產量、經改善之外觀、對植物病原體之經提高之抗性、經減少之病原性感染或其組合。 The plant seed of claim 12, wherein the plant growth benefit and/or the conferred protection is manifested by improved seedling vigor, improved root development, improved plant growth, enhanced plant health, Increased yield, improved appearance, increased resistance to plant pathogens, reduced pathogenic infection, or a combination thereof. 如請求項12之植物種子，其中該組合物包含解澱粉芽孢桿菌孢子之量係約1.0×102CFU/種子至約1.0×109CFU/種子。 The plant seed of claim 12, wherein the composition comprises B. amyloliquefaciens spores in an amount of from about 1.0 x 10 2 CFU per seed to about 1.0 x 10 9 CFU per seed. 如請求項12之植物種子，其中該種子包含以下之種子：單子葉植物、雙子葉植物、穀物、玉米、甜玉米、爆玉米、玉米種、青貯玉米、飼料玉米、稻穀、小麥、大麥、高粱、甘藍型蔬菜、椰菜、甘藍菜、花椰菜、芽甘藍、散葉甘蘭、羽衣甘藍、芥菜、球莖甘藍、鱗莖類蔬菜、洋蔥、大蒜、蔥、果蔬菜、胡椒、番茄、茄子、地面櫻桃、樹蕃茄、秋葵、葡萄、香草/香料、瓜類蔬菜、黃瓜、哈密瓜、甜瓜、香甘瓜、番南瓜、西瓜、南瓜、茄子、綠葉蔬菜、萵苣、芹菜、菠菜、香芹、菊 苣、豆科植物/蔬菜(多汁且乾燥之豆子及豌豆)、豆子、四季豆、食莢菜豆、殼豆、大豆、乾豆、回回豆、利馬豆、豌豆、鷹嘴豆、豌豆瓣、扁豆、油料種子作物、芥花、蓖麻、棉花、亞麻、花生、油菜籽、紅花、芝麻、向日葵、大豆、根/塊莖及球莖蔬菜、胡蘿蔔、馬鈴薯、甘薯、甜菜、薑、辣根、蘿蔔、人參、蕪菁、甘蔗、甜菜、草或草皮草。 The plant seed of claim 12, wherein the seed comprises the following seeds: monocot, dicot, grain, corn, sweet corn, popcorn, corn, silage corn, feed corn, rice, wheat, barley, sorghum , kale-type vegetables, broccoli, kale, broccoli, Brussels sprouts, broccoli, kale, mustard, broccoli, bulbous vegetables, onions, garlic, onions, fruit vegetables, pepper, tomato, eggplant, ground cherry, tree Tomato, okra, grape, vanilla/spice, melon, cucumber, cantaloupe, melon, cantaloupe, squash, watermelon, pumpkin, eggplant, green leafy vegetables, lettuce, celery, spinach, parsley, chrysanthemum Borage, legumes/vegetables (succulent and dry beans and peas), beans, green beans, pod beans, shell beans, soybeans, dried beans, peas, lima beans, peas, chickpeas, pea petals, Lentils, oilseed crops, canola, ramie, cotton, flax, peanuts, rapeseed, safflower, sesame, sunflower, soybean, roots/tubers and bulbous vegetables, carrots, potatoes, sweet potatoes, beets, ginger, horseradish, radish , ginseng, turnip, sugar cane, sugar beet, grass or turf grass. 如請求項14之植物種子，其中該植物包含以下之種子：乾豆、玉米、小麥、大豆、芥花、稻穀、黃瓜、胡椒、番茄、番南瓜、棉花、草或草皮草。 The plant seed of claim 14, wherein the plant comprises the following seeds: dried beans, corn, wheat, soybean, canola, rice, cucumber, pepper, tomato, squash, cotton, grass or turf grass. 如請求項12之植物種子，其中該組合物進一步包含存在適合於使植物生長獲益及/或對易感病植物賦予保護以避免病原性感染之量之以列之一或組合：微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑或植物生長調節劑。 The plant seed of claim 12, wherein the composition further comprises one or a combination of the amount of which is suitable for benefiting plant growth and/or conferring protection to the susceptible plant to avoid pathogenic infection: microorganisms, organisms Or chemical insecticides, fungicides, nematicides, fungicides or plant growth regulators. 一種用於達成使植物生長獲益或對易感病植物賦予保護以避免病原性感染中之一或兩者的組合物，該組合物包含：以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物，呈適合於使植物生長獲益及/或對該易感病植物賦予保護以避免病原性感染的量；以及微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑、除草劑、植物提取物、植物生長調節劑或肥料之一或組合，呈適合於使植物生長獲益及/或對該易感病植物賦予保護以避免病原性感染之量。 A composition for achieving one or both of the benefits of plant growth or protection of a susceptible plant to avoid pathogenic infection, the composition comprising: Bacillus amyloliquefaciens deposited under ATCC PTA-121166 RTI472 or a biologically pure culture of a mutant having all of its identifying characteristics, in an amount suitable for benefiting plant growth and/or conferring protection to the susceptible plant to avoid pathogenic infection; and microbial, biological or chemical One or a combination of an insecticide, a fungicide, a nematicide, a bactericide, a herbicide, a plant extract, a plant growth regulator or a fertilizer, which is suitable for benefiting plant growth and/or for a susceptible plant Give protection to avoid the amount of pathogenic infections. 如請求項18之組合物，其中該植物生長益處及/或該賦予之保護係藉由以下展現：經提高之幼苗活力、經改善之根發育、經改善之植物生長、經提高之植物健康、經增加之產量、經改善之 外觀、對植物病原體之經提高之抗性、經減少之病原性感染或其組合。 The composition of claim 18, wherein the plant growth benefit and/or the conferred protection is manifested by enhanced seedling vigor, improved root development, improved plant growth, enhanced plant health, Increased production, improved Appearance, increased resistance to plant pathogens, reduced pathogenic infection, or a combination thereof. 如請求項18之組合物，其中該組合物呈液體形式，且該解澱粉芽孢桿菌RTI472係以約1.0×108CFU/ml至約1.0×1012CFU/ml之濃度存在。 The composition of claim 18, wherein the composition is in liquid form, and the B. amyloliquefaciens RTI 472 is present at a concentration of from about 1.0 x 10 8 CFU/ml to about 1.0 x 10 12 CFU/ml. 如請求項18之組合物，其中該組合物呈粉劑、乾燥可潤濕粉末、可撒佈顆粒或乾燥可潤濕顆粒形式，且該解澱粉芽孢桿菌RTI472係以約1.0×108CFU/g至約1.0×1012CFU/g之量存在。 The composition of claim 18, wherein the composition is in the form of a powder, a dry wettable powder, a spreadable granule or a dry wettable granule, and the B. amyloliquefaciens RTI 472 is about 1.0 x 10 8 CFU/g. It is present in an amount of about 1.0 x 10 12 CFU/g. 如請求項18之組合物，其中該組合物呈油性分散液形式，且該解澱粉芽孢桿菌RTI472係以約1.0×108CFU/ml至約1.0×1012CFU/ml之濃度存在。 The composition of claim 18, wherein the composition is in the form of an oily dispersion, and the B. amyloliquefaciens RTI 472 is present at a concentration of from about 1.0 x 10 8 CFU/ml to about 1.0 x 10 12 CFU/ml. 如請求項18之組合物，其中該解澱粉芽孢桿菌RTI472呈孢子或營養細胞形式。 The composition of claim 18, wherein the B. amyloliquefaciens RTI 472 is in the form of spores or vegetative cells. 如請求項18之組合物，其中該植物包含單子葉植物、雙子葉植物、穀物、玉米、甜玉米、爆玉米、玉米種、青貯玉米、飼料玉米、稻穀、小麥、大麥、高粱、蘆筍、漿果、藍莓、黑莓、樹莓、羅甘莓、越橘、蔓越橘、醋栗、接骨木、黑醋栗、蔓越莓、越莓、甘藍型蔬菜、椰菜、甘藍菜、花椰菜、芽甘藍、散葉甘藍、羽衣甘藍、芥菜、球莖甘藍、瓜類蔬菜、黃瓜、哈密瓜、甜瓜、香甘瓜、番南瓜、西瓜、南瓜、茄子、鱗莖類蔬菜、洋蔥、大蒜、蔥、陳皮、橘子、葡萄柚、檸檬、紅橘、橘柚、柚子、果蔬菜、胡椒、番茄、地面櫻桃、樹蕃茄、秋葵、葡萄、香草/香料、綠葉蔬菜、萵苣、芹菜、菠菜、香芹、菊苣、豆科植物/蔬菜(多汁且乾燥之豆子及豌豆)、豆子、四季豆、食莢菜豆、殼豆、大豆、乾豆、回回豆、利馬豆、豌豆、鷹嘴豆、豌豆瓣、扁豆、油料種子作物、芥花、蓖麻、椰子、 棉花、亞麻、油棕櫚、橄欖、花生、油菜籽、紅花、芝麻、向日葵、大豆、梨果、蘋果、海棠果、梨、榅桲、夏花山楂、根/塊莖及球莖蔬菜、胡蘿蔔、馬鈴薯、甘薯、木薯、甜菜、薑、辣根、蘿蔔、人參、蕪菁、核果、杏子、櫻桃、油桃、桃子、李子、梅子、草莓、堅果、杏仁、開心果、山核桃、核桃、榛子、板栗、腰果、山毛櫸堅果、胡桃、夏威夷果、獼猴桃、香蕉、(藍)龍舌蘭、草、草皮草、觀賞植物、一品紅、硬木剪枝、栗樹、橡樹、楓樹、甘蔗或甜菜。 The composition of claim 18, wherein the plant comprises monocots, dicots, grains, corn, sweet corn, popcorn, corn, silage corn, feed corn, rice, wheat, barley, sorghum, asparagus, berries , blueberry, blackberry, raspberry, raspberry, cowberry, cranberry, gooseberry, elderberry, black currant, cranberry, raspberry, kale, broccoli, kale, broccoli, Brussels sprouts , broccoli, kale, mustard, stalks, melons, cucumbers, cantaloupe, melon, cantaloupe, squash, watermelon, pumpkin, eggplant, bulbous vegetables, onions, garlic, onions, tangerine peel, oranges, Grapefruit, lemon, red orange, tangerine, grapefruit, fruit vegetable, pepper, tomato, ground cherry, tree tomato, okra, grape, vanilla/spice, green leafy vegetables, lettuce, celery, spinach, parsley, chicory, beans Plants/vegetables (succulent and dry beans and peas), beans, green beans, pod beans, shell beans, soybeans, dried beans, peas, lima beans, peas, chickpeas, pea petals, lentils, oil Seed crop, canola, castor, coconut, Cotton, flax, oil palm, olive, peanut, rapeseed, safflower, sesame, sunflower, soybean, pear, apple, jellyfish, pear, alfalfa, summer flower hawthorn, roots/tubers and bulbous vegetables, carrots, potatoes, Sweet potato, cassava, beet, ginger, horseradish, radish, ginseng, turnip, stone fruit, apricot, cherry, nectarine, peach, plum, plum, strawberry, nut, almond, pistachio, pecan, walnut, hazelnut, chestnut, Cashew nuts, beech nuts, walnuts, macadamia nuts, kiwis, bananas, (blue) agave, grass, turfgrass, ornamental plants, poinsettia, hardwood pruning, chestnut, oak, maple, sugar cane or beet. 一種用於達成使植物生長獲益或對易感病植物賦予保護以避免病原性感染中之一或兩者的方法，該方法包含：將如下組合物輸送至：該植物之葉、該植物之莖皮、該植物之果實、該植物之花、該植物之種子、該植物之根部、該植物之剪枝、該植物之移植物、該植物之癒合組織；該植物周圍土壤或生長介質；在土壤或生長介質中播種該植物之種子之前的該土壤或生長介質；或在土壤或生長介質中種植該植物、該植物剪枝、該植物移植物或該植物癒合組織之前的該土壤或生長介質，該組合物包含以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物，呈適合於使該植物生長獲益及/或對該易感病植物賦予保護以避免病原性感染之量。 A method for achieving one or both of plant growth benefit or protection of a susceptible plant to avoid a pathogenic infection, the method comprising: delivering a composition to: a leaf of the plant, the plant Stem bark, fruit of the plant, flower of the plant, seed of the plant, root of the plant, pruning of the plant, graft of the plant, healing tissue of the plant; soil or growth medium surrounding the plant; The soil or growth medium prior to seeding the seed of the plant in the soil or growth medium; or planting the plant, the plant pruner, the plant graft or the soil or growth medium prior to healing the tissue in the soil or growth medium The composition comprises a B. amyloliquefaci RTI 472 deposited with ATCC No. PTA-121166 or a biologically pure culture thereof having all of its identifying characteristics, which is suitable for benefiting the growth of the plant and/or The susceptible plant confers protection against the amount of pathogenic infection. 如請求項25之方法，其中該組合物輸送至該植物之葉。 The method of claim 25, wherein the composition is delivered to the leaves of the plant. 如請求項25之方法，其中該植物之生長益處及/或該賦予之保護係藉由以下展現：經提高之幼苗活力、經改善之根發育、經改善之植物生長、經提高之植物健康、經增加之產量、經改善之外觀、對植物病原體之經提高之抗性、經減少之病原性感染或其組合。 The method of claim 25, wherein the growth benefit of the plant and/or the protection conferred by the plant is manifested by improved seedling vigor, improved root development, improved plant growth, enhanced plant health, Increased yield, improved appearance, increased resistance to plant pathogens, reduced pathogenic infection, or a combination thereof. 如請求項25之方法，其中該解澱粉芽孢桿菌RTI472係以約1.0×1010CFU/ha至約1.0×1014CFU/ha之速率輸送。 The method of claim 25, wherein the B. amyloliquefaciens RTI 472 is delivered at a rate of from about 1.0 x 10 10 CFU/ha to about 1.0 x 10 14 CFU/ha. 如請求項25之方法，其中該解澱粉芽孢桿菌RTI472呈孢子或營養細胞形式。 The method of claim 25, wherein the B. amyloliquefaciens RTI 472 is in the form of spores or vegetative cells. 如請求項25之方法，其中該組合物呈液體、粉劑、乾燥可濕潤粉末、可撒佈顆粒、乾燥可濕潤顆粒或油性分散液形式。 The method of claim 25, wherein the composition is in the form of a liquid, a powder, a dry wettable powder, a spreadable granule, a dry wettable granule or an oily dispersion. 如請求項25之方法，其中該組合物進一步包含存在適合於使植物生長獲益及/或對該易感病植物賦予保護以避免病原性感染之量之以下之一或組合：微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑、除草劑、植物提取物、植物生長調節劑或肥料。 The method of claim 25, wherein the composition further comprises one or a combination of the presence of an amount suitable for benefiting plant growth and/or conferring protection to the susceptible plant to avoid pathogenic infection: microorganism, organism or Chemical insecticides, fungicides, nematicides, fungicides, herbicides, plant extracts, plant growth regulators or fertilizers. 如請求項25之方法，其中該組合物進一步包含以下之一或組合：載劑、界面活性劑、分散劑或酵母提取物。 The method of claim 25, wherein the composition further comprises one or a combination of: a carrier, a surfactant, a dispersant, or a yeast extract. 如請求項25之方法，其中該植物包含單子葉植物、雙子葉植物、穀物、玉米、甜玉米、爆玉米、玉米種、青貯玉米、飼料玉米、稻穀、小麥、大麥、高粱、蘆筍、漿果、藍莓、黑莓、樹莓、羅甘莓、越橘、蔓越橘、醋栗、接骨木、黑醋栗、蔓越莓、越莓、甘藍型蔬菜、椰菜、甘藍菜、花椰菜、芽甘藍、散葉甘藍、羽衣甘藍、芥菜、球莖甘藍、瓜類蔬菜、黃瓜、哈密瓜、甜瓜、香甘瓜、番南瓜、西瓜、南瓜、茄子、鱗莖類蔬菜、洋蔥、大蒜、蔥、陳皮、橘子、葡萄柚、檸檬、紅橘、橘抽、柚子、果蔬菜、胡椒、番茄、地面櫻桃、樹蕃茄、秋葵、葡萄、香草/香料、綠葉蔬菜、萵苣、芹菜、菠菜、香芹、菊苣、豆科植物/蔬菜(多汁且乾燥之豆子及豌豆)、豆子、四季豆、食莢菜豆、殼豆、大豆、乾豆、回回豆、利馬豆、豌豆、鷹嘴豆、豌豆瓣、扁豆、油料種子作物、芥花、蓖麻、椰子、 棉花、亞麻、油棕櫚、橄欖、花生、油菜籽、紅花、芝麻、向日葵、大豆、梨果、蘋果、海棠果、梨、榅桲、夏花山楂、根/塊莖及球莖蔬菜、胡蘿蔔、馬鈴薯、甘薯、木薯、甜菜、薑、辣根、蘿蔔、人參、蕪菁、核果、杏子、櫻桃、油桃、桃子、李子、梅子、草莓、堅果、杏仁、開心果、山核桃、核桃、榛子、板栗、腰果、山毛櫸堅果、胡桃、夏威夷果、獼猴桃、香蕉、(藍)龍舌蘭、草、草皮草、觀賞植物、一品紅、硬木剪枝、栗樹、橡樹、楓樹、甘蔗或甜菜。 The method of claim 25, wherein the plant comprises monocots, dicots, grains, corn, sweet corn, popcorn, corn, silage corn, forage corn, rice, wheat, barley, sorghum, asparagus, berries, Blueberries, blackberries, raspberries, raspberries, cranberries, cranberries, gooseberries, elderberries, currants, cranberries, berry, cabbage, broccoli, kale, broccoli, Brussels sprouts, Cabbage, kale, mustard, stalk, melon, cucumber, cantaloupe, melon, sweet melon, squash, watermelon, pumpkin, eggplant, bulbous vegetables, onions, garlic, onions, tangerine peel, oranges, grapes Pomelo, lemon, red orange, orange pump, grapefruit, fruit vegetable, pepper, tomato, ground cherry, tree tomato, okra, grape, vanilla/spice, green leafy vegetables, lettuce, celery, spinach, parsley, chicory, legume Plants/vegetables (succulent and dry beans and peas), beans, green beans, pod beans, shell beans, soybeans, dried beans, peas, lima beans, peas, chickpeas, peas, lentils, oil Sub-crop, canola, castor, coconut, Cotton, flax, oil palm, olive, peanut, rapeseed, safflower, sesame, sunflower, soybean, pear, apple, jellyfish, pear, alfalfa, summer flower hawthorn, roots/tubers and bulbous vegetables, carrots, potatoes, Sweet potato, cassava, beet, ginger, horseradish, radish, ginseng, turnip, stone fruit, apricot, cherry, nectarine, peach, plum, plum, strawberry, nut, almond, pistachio, pecan, walnut, hazelnut, chestnut, Cashew nuts, beech nuts, walnuts, macadamia nuts, kiwis, bananas, (blue) agave, grass, turfgrass, ornamental plants, poinsettia, hardwood pruning, chestnut, oak, maple, sugar cane or beet. 如請求項33之方法，其中該植物包含大豆、豆子、食莢菜豆、小麥、棉花、玉米、胡椒、番茄、馬鈴薯、木薯、葡萄、草莓、香蕉、花生、番南瓜、南瓜、茄子或黃瓜。 The method of claim 33, wherein the plant comprises soybean, bean, pod bean, wheat, cotton, corn, pepper, tomato, potato, tapioca, grape, strawberry, banana, peanut, squash, pumpkin, eggplant or cucumber. 如請求項25之方法，其中該病原性感染由以下引起：植物真菌病原體、植物細菌病原體、鏽菌、葡萄孢屬(Botrytis spp.)、灰葡萄孢菌(Botrytis cinerea)、蔥鱗葡萄孢菌(Botrytis squamosa)、歐文菌屬(Erwinia spp.)、胡蘿蔔軟腐歐文菌(Erwinia carotovora)、解澱粉歐文菌(Erwinia amylovora)、狄克氏菌屬(Dickeya spp.)、達旦狄克氏菌(Dickeya dadantii)、黑脛病菌(Dickeya solani)、土壤桿菌屬(Agrobacterium spp.)、根癌土壤桿菌(Agrobacterium tumefaciens)、黃單孢菌屬(Xanthomonas spp.)、地毯草黃單孢菌(Xanthomonas axonopodis)、油菜黃單孢菌胡蘿蔔致病變種(Xanthomonas campestris pv.carotae)、桃李黃單孢菌(Xanthomonas pruni)、核桃黃單孢菌(Xanthomonas arboricola)、水稻黃單孢菌水稻變種(Xanthomonas oryzae pv.oryzae)、焦枯菌屬(Xylella spp.)、葉緣焦枯菌(Xylella fastidiosa)、卡地菌屬(Candidatus spp.)、韌皮部桿菌(Candidatus liberibacter)、鐮刀菌屬(Fusarium spp.)、大刀鐮刀菌(Fusarium colmorum)、禾穀鐮刀 菌(Fusarium graminearum)、尖孢鐮刀菌(Fusarium oxysporum)、古巴尖孢鐮刀菌(Fusarium oxysporum f.sp.Cubense)、番茄尖孢鐮刀菌(Fusarium oxysporum f.sp.Lycopersici)、棒形鐮刀菌(Fusarium virguliforme)、核盤菌屬(Sclerotinia spp.)、向日葵核盤菌(Sclerotinia sclerotiorum)、小核盤菌(Sclerotinia minor)、同果核盤菌(Sclerotinia homeocaspa)、尾孢菌屬(Cercospora/Cercosporidium spp.)、鉤絲殼屬(Uncinula spp.)、葡萄白粉病菌(Uncinula necator)(白粉病(Powdery Mildew))、叉絲單囊殼屬(Podosphaera spp.)(白粉病)、白叉絲單囊殼(Podosphaera leucotricha)、克蘭德叉絲單囊殼(Podosphaera clandestine)、擬莖點菌屬(Phomopsis spp.)、葡萄擬莖點黴病菌(Phomopsis viticola)、交鏈孢菌屬(Alternaria spp.)、細極鏈格孢菌(Alternaria tenuissima)、蔥鏈格孢菌(Alternaria porri)、交鏈格孢菌(Alternaria alternate)、立枯交鏈孢菌(Alternaria solani)、鏈格孢菌(Alternaria tenuis)、假單胞菌屬(Pseudomonas spp.)、丁香假單胞菌番茄變種(Pseudomonas syringae pv.Tomato)、疫黴菌屬(Phytophthora spp.)、致病疫黴菌(Phytophthora infestans)、寄生疫黴菌(Phytophthora parasitica)、大豆疫黴菌(Phytophthora sojae)、辣椒疫黴菌(Phytophthora capsici)、樟疫黴菌(Phytophthora cinnamon)、草莓疫黴菌(Phytophthora fragariae)、疫黴菌屬(Phytophthora spp.)、櫟樹猝死病菌(Phytophthora ramorum)、棕櫚疫黴菌(Phytophthora palmivara)、菸草疫黴菌(Phytophthora nicotianae)、層鏽菌屬(Phakopsora spp.)、豆薯層鏽菌(Phakopsora pachyrhizi)、層鏽層假尾孢菌(Phakopsora meibomiae)、曲黴菌屬(Aspergillus spp.)、黃麴黴(Aspergillus flavus)、黑麯黴(Aspergillus niger)、單孢鏽菌屬(Uromyces spp.)、菜豆銹病菌(Uromyces appendiculatus)、枝孢菌屬(Cladosporium spp.)、多主枝孢黴(Cladosporium herbarum)、根黴菌屬(Rhizopus spp.)、少根根黴菌(Rhizopus arrhizus)、青黴菌屬(Penicillium spp.)、絲核菌屬(Rhizoctonia spp.)、立枯絲核菌(Rhizoctonia solani)、玉蜀黍絲核菌(Rhizoctonia zeae)、稻枯斑絲核菌(Rhizoctonia oryzae)、褐色絲核菌(Rhizoctonia caritae)、禾穀絲核菌(Rhizoctonia cerealis)、紫紋羽絲核菌(Rhizoctonia crocorum)、草莓絲核菌(Rhizoctonia fragariae)、枝生絲核菌(Rhizoctonia ramicola)、覆盆子絲核菌(Rhizoctonia rubi)、豆狀絲核菌(Rhizoctonia leguminicola)、菜豆殼球孢菌(Macrophomina phaseolina)、稻瘟菌(Magnaorthe oryzae)、球腔菌屬(Mycosphaerella spp.)、禾生球腔菌(Mycosphaerella graminocola)、斐濟球腔菌(Mycosphaerella fijiensis)(黑色香蕉葉斑病(Black sigatoga))、蘋果斑點球腔菌(Mycosphaerella pomi)、檸檬球腔菌(Mycosphaerella citri)、稻瘟菌屬(Magnaporthe spp.)、稻瘟病菌(Magnaporthe grisea)、鏈核盤菌屬(Monilinia spp.)、桃褐腐病菌(Monilinia fruticola)、藍莓僵果病病原菌(Monilinia vacciniicorymbosi)、核果鏈核盤菌(Monilinia laxa)、炭疽菌屬(Colletotrichum spp.)、膠孢炭疽菌(Colletotrichum gloeosporiodes)、辣椒炭疽菌(Colletotrichum acutatum)、石斛炭疽菌(Colletotrichum Candidum)、間座殼屬(Diaporthe spp.)、柑桔間座殼菌(Diaporthe citri)、棒孢菌屬(Corynespora spp.)、多主棒孢菌(Corynespora Cassiicola)、膠鏽菌屬(Gymnosporangium spp.)、植物受檜膠鏽菌(Gymnosporangium juniperi-virginianae)、裂盾菌屬(Schizothyrium spp.)、仁果裂盾菌(Schizothyrium pomi)、黏殼孢菌屬(Gloeodes spp.)、梨果黏殼孢菌(Gloeodes pomigena)、葡萄座腔菌屬(Botryosphaeria spp.)、葡萄潰瘍病病菌(Botryosphaeria dothidea)、明孢盤菌屬(Neofabraea spp.)、威爾遜孢殼菌屬(Wilsonomyces spp.)、嗜果威爾遜孢殼菌(Wilsonomyces carpophilus)、單囊殼菌屬(Sphaerotheca spp.)、斑點單囊殼菌(Sphaerotheca macularis)、薔薇單囊殼菌(Sphaerotheca pannosa)、白粉菌屬(Erysiphe spp.)、殼多孢菌屬(Stagonospora spp.)、穎枯殼針孢(Stagonospora nodorum)、腐黴屬(Pythium spp.)、終極腐黴菌(Pythium ultimum)、瓜果腐黴菌(Pythium aphanidermatum)、畸雌腐黴菌(Pythium irregularum)、簇囊腐黴菌(Pythium ulosum)、水生腐黴菌(Pythium lutriarium)、森林腐黴菌(Pythium sylvatium)、黑星菌屬(Venturia spp.)、蘋果黑星病菌(Venturia inaequalis)、輪枝孢菌屬(Verticillium spp.)、黑粉菌屬(Ustilago spp.)、裸黑粉菌(Ustilago nuda)、玉蜀黍黑粉菌(Ustilago maydis)、甘蔗鞭黑粉菌(Ustilago scitaminea)、麥角菌屬(Claviceps spp.)、麥角菌(Claviceps puprrea)、腥黑粉菌屬(Tilletia spp.)、小麥腥黑粉菌(Tilletia tritici)、光滑腥黑粉菌(Tilletia laevis)、稻粒黑粉菌(Tilletia horrid)、小麥矮腥黑粉菌(Tilletia controversa)、莖點黴屬(Phoma spp.)、大豆生莖點黴(Phoma glycinicola)、多變莖點黴(Phoma exigua)、甘藍莖點黴(Phoma lingam)、禾旋孢腔菌(Cocliobolus sativus)、禾頂囊殼(Gaeumanomyces gaminis)、炭疽菌屬(Colleototricum spp.)、雲紋病菌屬(Rhychosporium spp.)、大麥雲紋病菌(Rhychosporium secalis)、離蠕孢屬(Biopolaris spp.)、長蠕孢屬(Helminthosporium spp.)、大麥長蠕孢(Helminthosporium secalis)、玉蜀黍長蠕抱(Helminthosporium maydis)、索萊長蠕抱(Helminthosporium solai)、小麥褐斑長蠕孢(Helminthosporium tritici-repentis)或其組合。 The method of claim 25, wherein the pathogenic infection is caused by a plant fungal pathogen, a plant bacterial pathogen, a rust fungus, Botrytis spp. , Botrytis cinerea , and Botrytis cinerea ( Botrytis squamosa ), Erwinia spp. , Erwinia carotovora , Erwinia amylovora , Dickeya spp. , Dickeya Dadantii ), Dickeya solani , Agrobacterium spp. , Agrobacterium tumefaciens , Xanthomonas spp. , Xanthomonas axonopodis Xanthomonas campestris pv. carotae , Xanthomonas pruni , Xanthomonas arboricola , Xanthomonas oryzae pv. Oryzae ), Xylella spp. , Xylella fastidiosa , Candidatus spp., phloem ( C) Andidatus liberibacter ), Fusarium spp. , Fusarium colmorum , Fusarium graminearum , Fusarium oxysporum , Fusarium oxysporum f.sp .Cubense ), Fusarium oxysporum f.sp. Lycopersici , Fusarium virguliforme , Sclerotinia spp. , Sclerotinia sclerotiorum , small nucleus Sclerotinia minor , Sclerotinia homeocaspa , Cercospora/Cercosporidium spp., Uncinula spp., Uncinula necator (Powdery) Mildew)), Podosphaera spp. ( powder disease ), white stalk shell ( Podosphaera leucotricha ), Claude stalked shell ( Podosphaera clandestine ), Pseudomonas sp. Phomopsis spp.), Phomopsis viticola , Alternaria spp. , Alternaria tenuissima , Alterna solani ( Alterna) Ria porri ), Alternaria alternate , Alternaria solani , Alternaria tenuis , Pseudomonas spp., Pseudomonas syringae Tomato cultivar ( Pseudomonas syringae pv. Tomato ), Phytophthora spp., Phytophthora infestans , Phytophthora parasitica , Phytophthora sojae , Phytophthora capsici ), Phytophthora cinnamon , Phytophthora fragariae , Phytophthora spp. , Phytophthora ramorum , Phytophthora palmivara , Phytophthora nicotianae ), Phakopsora spp., Phakopsora pachyrhizi , Phakopsora meibomiae , Aspergillus spp. , Aspergillus flavus , Aspergillus niger , Uromyces spp. , Uromyces appendiculatus ), Cladosporium spp., Cladosporium herbarum , Rhizopus spp., Rhizopus arrhizus , Penicillium spp., silk Rhizoctonia spp. , Rhizoctonia solani , Rhizoctonia zeae, Rhizoctonia oryzae , Rhizoctonia caritae , cereal Rhizoctonia cerealis , Rhizoctonia crocorum , Rhizoctonia fragariae , Rhizoctonia ramicola , Rhizoctonia rubi , Beanlike silk solani (Rhizoctonia leguminicola), bean shell fungus Beauveria (Macrophomina phaseolina), Pyricularia oryzae (Magnaorthe oryzae), Mycosphaerella (Mycosphaerella spp.), Mycosphaerella graminicola (Mycosphaerella graminocola), Fiji Leptosphaeria (Mycosphaerella fijiensis) (black Sigatoka (black sigatoga)), apple scab Leptosphaeria (Mycosphaerella pomi), lemon Leptosphaeria (Mycosphaerella citri), rice blast species (M Agnaporthe spp. ), Magnaporthe grisea , Monilinia spp., Monilinia fruticola , Monilinia vacciniicorymbosi , Monilinia Laxa ), Colletotrichum spp., Colletotrichum gloeosporiodes , Colletotrichum acutatum , Colletotrichum Candidum , Diaporthe spp., Citrus Block globosum (Diaporthe citri), the genus Micromonospora rod (Corynespora spp.), multi-master rod cinerea (Corynespora Cassiicola), the genus gum rust (Gymnosporangium spp.), gum juniper plants by rust (Gymnosporangium juniperi-virginianae) , Schizothyrium spp., Schizothyrium pomi , Gloeodes spp., Gloeodes pomigena , Botryosphaeria Spp .), Botryosphaeria dothidea , Neofabraea spp., Wilsonomyces spp., fruiting Wilson Wilsonomyces carpophilus , Sphaerotheca spp., Sphaerotheca macularis , Sphaerotheca pannosa , Erysiphe spp., shell Stagonospora spp. , Stagonospora nodorum , Pythium spp. , Pythium ultimum , Pythium aphanidermatum, Pythium pyogenes Pythium irregularum ), Pythium ulosum , Pythium lutriarium , Pythium sylvatium , Venturia spp. , Venturia inaequalis , round branches Verticillium spp., Ustilago spp. , Ustilago nuda , Ustilago maydis , Ustilago scitaminea , ergot Genus ( Claviceps spp. ), Claviceps puprrea , Tilletia spp. , Tilletia tritici , Tilletia laevis , rice black Tilletia horrid , Tilletia controversa , Phoma spp. , Phoma glycinicola , Phoma exigua , Cabbage stalk Phoma lingam , Cocliobolus sativus , Gaeumanomyces gaminis , Colleototricum spp., Rhychosporium spp. , Barley grisea Rhychosporium secalis ), Biopolaris spp. , Helminthosporium spp. , Helminthosporium secalis , Helminthosporium maydis , Soleil long sling ( Helminthosporium solai ), Helminthosporium tritici-repentis or a combination thereof. 如請求項35之方法，其中該病原性感染由以下之一或組合引起：大豆鏽菌(豆薯層鏽菌、層鏽層假尾孢菌)且該植物包含大豆；灰葡萄孢菌(葡萄孢疫病)且該植物包含葡萄；灰葡萄孢菌(葡萄孢疫病)且該植物包含草莓；交鏈孢菌屬(例如立枯交鏈孢菌)且該植物包含番茄；交鏈孢菌屬(例如立枯交鏈孢菌)且該植物包含馬鈴薯；豆銹病(菜豆銹病菌)且該植物包含常見豆子；白粉病菌(大豆白粉病)且該植物包含大豆；斐濟球腔菌(黑色香蕉葉斑病)或古巴尖孢鐮刀菌(巴拿馬疾病)且該植物包含香蕉；黃單孢菌屬或水稻黃單孢菌水稻變種且該植物包含稻穀；地毯草黃單孢菌且該植物包含木薯；野油菜黃單孢菌且該植物包含番茄；灰葡萄孢菌(胡椒葡萄孢疫病)且該植物包含胡椒；白粉病且該植物包含葫蘆；向日葵核盤菌(白黴)且該植物包含食莢菜豆；向日葵核盤菌(白黴)且該植物包含馬鈴薯；同果核盤菌(幣斑病)且該植物包含草皮草；南方白黴且該植物包含花生；葉斑病(尾孢菌/尾孢菌)且該植物包含花生；禾穀鐮刀菌(小麥赤黴病)且該植物包含小麥；禾生球腔菌(小麥殼針孢斑)且該植物包含小麥；穎枯殼針孢(穎枯病及殼針孢葉枯病)且該植物包含小麥；解澱粉歐文菌且該植物包含蘋果、梨及其他梨果果實； 蘋果黑星病菌且該植物包含蘋果、梨及其他梨果果實；或立枯絲核菌且該植物包含小麥、稻穀、草皮草、大豆、玉米、豆科植物及蔬菜作物。 The method of claim 35, wherein the pathogenic infection is caused by one or a combination of: soybean rust (Pythium humilis, Pseudomonas aeruginosa) and the plant comprises soybean; Botrytis cinerea (grape) Spore plague) and the plant comprises a grape; Botrytis cinerea (B. blight) and the plant comprises a strawberry; a genus Alternaria (eg, Alternaria solani) and the plant comprises a tomato; For example, Alternaria solani) and the plant comprises potato; bean rust (String rust) and the plant contains common beans; powdery mildew (soybean powdery mildew) and the plant contains soybean; Ficus sclerotium (black banana leaf spot) Disease) or Fusarium oxysporum (Panama disease) and the plant comprises banana; Xanthomonas or Xanthomonas oryzae rice variety and the plant comprises rice; Trichophyton rubrum and the plant contains cassava; Xanthomonas camphora and the plant comprises tomato; Botrytis cinerea (P. cinerea) and the plant comprises pepper; powdery mildew and the plant comprises a gourd; S. cerevisiae (White mold) and the plant comprises a pod bean Sunflower Sclerotium (White mold) The plant comprises potato; Sclerotinia sclerotiorum (coin spot disease) and the plant comprises turfgrass; southern white mold and the plant comprises peanut; leaf spot disease (S. cerevisiae / Cercospora) and the plant comprises peanuts; Fusarium oxysporum (Schizophrenia) and the plant comprises wheat; Phytophthora sinensis (S. cerevisiae) and the plant comprises wheat; Phytophthora capsici (Bacterial blight and Phytophthora capsici) The plant comprises wheat; the starch-degraded Owen bacteria and the plant comprises apple, pear and other pear fruit; Apple black spot disease and the plant comprises apple, pear and other pear fruit; or Rhizoctonia solani and the plant comprises wheat, rice, turf, soybean, corn, legume and vegetable crops. 一種用於達成使植物生長獲益或對易感病植物賦予保護以避免病原性感染中之一或兩者的方法，該方法包含：將該植物之種子種植在適合生長介質中或使該植物之營養剪枝/組織在適合生長介質中再生，其中該種子已用如下組合物塗佈或該營養剪枝/組織業經該組合物接種，該組合物包含以ATCC PTA-121166寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物，其中該植物的生長可獲益自該種子或該營養剪枝/組織，及/或賦予保護以避免病原性感染。 A method for achieving one or both of plant growth benefits or protection of susceptible plants to avoid pathogenic infections, the method comprising: planting the seeds of the plants in a suitable growth medium or causing the plants The nutrient pruning/tissue is regenerated in a suitable growth medium, wherein the seed has been coated with a composition or the nutritional pruning/tissue is inoculated with the composition comprising a starch spore deposited with ATCC PTA-121166 Bacillus RTI 472 or a biologically pure culture of a mutant having all of its identifying characteristics, wherein the growth of the plant may benefit from the seed or the nutritional pruning/tissue, and/or confer protection to avoid pathogenic infection. 如請求項37之方法，其中該植物生長益處係藉由以下展現：經提高之幼苗活力、經改善之根發育、經改善之植物生長、經提高之植物健康、經增加之產量、經改善之外觀、對植物病原體之經提高之抗性、經減少之病原性感染或其組合。 The method of claim 37, wherein the plant growth benefit is manifested by improved seedling vigor, improved root development, improved plant growth, improved plant health, increased yield, improved Appearance, increased resistance to plant pathogens, reduced pathogenic infection, or a combination thereof. 如請求項37之方法，其中該解澱粉芽孢桿菌RTI472係以約1.0×102CFU/種子至約1.0×109CFU/種子之量之孢子形式存在。 The method of claim 37, wherein the B. amyloliquefaciens RTI 472 is present in the form of spores in an amount of from about 1.0 x 10 2 CFU per seed to about 1.0 x 10 9 CFU per seed. 如請求項37之方法，其中該植物包含單子葉植物、雙子葉植物、穀物、玉米、甜玉米、爆玉米、玉米種、青貯玉米、飼料玉米、稻穀、小麥、大麥、高粱、蘆筍、漿果、藍莓、黑莓、樹莓、羅甘莓、越橘、蔓越橘、醋栗、接骨木、黑醋栗、蔓越莓、越莓、甘藍型蔬菜、椰菜、甘藍菜、花椰菜、芽甘藍、散葉甘藍、羽衣甘藍、芥菜、球莖甘藍、瓜類蔬菜、黃瓜、哈密瓜、甜瓜、香甘瓜、番南瓜、西瓜、南瓜、茄子、鱗莖類蔬菜、洋蔥、大蒜、蔥、陳皮、橘子、葡萄柚、檸檬、紅橘、橘柚、柚子、果蔬菜、胡椒、番茄、地面櫻桃、樹蕃茄、秋葵、 葡萄、香草/香料、綠葉蔬菜、萵苣、芹菜、菠菜、香芹、菊苣、豆科植物/蔬菜(多汁且乾燥之豆子及豌豆)、豆子、四季豆、食莢菜豆、殼豆、大豆、乾豆、回回豆、利馬豆、豌豆、鷹嘴豆、豌豆瓣、扁豆、油料種子作物、芥花、蓖麻、椰子、棉花、亞麻、油棕櫚、橄欖、花生、油菜籽、紅花、芝麻、向日葵、大豆、梨果、蘋果、海棠果、梨、榅桲、夏花山楂、根/塊莖及球莖蔬菜、胡蘿蔔、馬鈴薯、甘薯、木薯、甜菜、薑、辣根、蘿蔔、人參、蕪菁、核果、杏子、櫻桃、油桃、桃子、李子、梅子、草莓、堅果、杏仁、開心果、山核桃、核桃、榛子、板栗、腰果、山毛櫸堅果、胡桃、夏威夷果、獼猴桃、香蕉、(藍)龍舌蘭、草、草皮草、觀賞植物、一品紅、硬木剪枝、栗樹、橡樹、楓樹、甘蔗或甜菜。 The method of claim 37, wherein the plant comprises monocots, dicots, grains, corn, sweet corn, popcorn, corn, silage corn, forage corn, rice, wheat, barley, sorghum, asparagus, berries, Blueberries, blackberries, raspberries, raspberries, cranberries, cranberries, gooseberries, elderberries, currants, cranberries, berry, cabbage, broccoli, kale, broccoli, Brussels sprouts, Cabbage, kale, mustard, stalk, melon, cucumber, cantaloupe, melon, sweet melon, squash, watermelon, pumpkin, eggplant, bulbous vegetables, onions, garlic, onions, tangerine peel, oranges, grapes Pomelo, lemon, red tangerine, tangerine, grapefruit, fruit and vegetable, pepper, tomato, ground cherry, tree tomato, okra, Grapes, herbs/spice, green leafy vegetables, lettuce, celery, spinach, parsley, chicory, legumes/vegetables (succulent and dry beans and peas), beans, green beans, food pods, shell beans, soybeans, Dried beans, peas, lima beans, peas, chickpeas, pea petals, lentils, oilseed crops, canola, ramie, coconut, cotton, flax, oil palm, olive, peanut, rapeseed, safflower, sesame, Sunflower, soybean, pear fruit, apple, jellyfish, pear, alfalfa, summer flower hawthorn, root/tuber and bulbous vegetables, carrot, potato, sweet potato, cassava, beet, ginger, horseradish, radish, ginseng, turnip, stone fruit , apricot, cherry, nectarine, peach, plum, plum, strawberry, nut, almond, pistachio, pecan, walnut, hazelnut, chestnut, cashew, beech nut, walnut, macadamia, kiwi, banana, (blue) dragon Tequila, grass, turf grass, ornamental plants, poinsettia, hardwood pruning, chestnut, oak, maple, sugar cane or beet. 如請求項39之方法，其中該植物包含大豆、豆子、食莢菜豆、小麥、棉花、玉米、胡椒、番茄、馬鈴薯、木薯、葡萄、草莓、香蕉、花生、番南瓜、南瓜、茄子或黃瓜。 The method of claim 39, wherein the plant comprises soybean, bean, pod bean, wheat, cotton, corn, pepper, tomato, potato, tapioca, grape, strawberry, banana, peanut, squash, pumpkin, eggplant or cucumber. 如請求項37之方法，其中該病原性感染由以下引起：植物真菌病原體、植物細菌病原體、鏽菌、葡萄孢屬、灰葡萄孢菌、蔥鱗葡萄孢菌、歐文菌屬、胡蘿蔔軟腐歐文菌、解澱粉歐文菌、狄克氏菌屬、達旦狄克氏菌、黑脛病菌、土壤桿菌屬、根癌土壤桿菌、黃單孢菌屬、地毯草黃單孢菌、油菜黃單孢菌胡蘿蔔致病變種、桃李黃單孢菌、核桃黃單孢菌、水稻黃單孢菌水稻變種、焦枯菌屬、葉緣焦枯菌、卡地菌屬、韌皮部桿菌、鐮刀菌屬、大刀鐮刀菌、禾穀鐮刀菌、尖孢鐮刀菌、古巴尖孢鐮刀菌、番茄尖孢鐮刀菌、棒形鐮刀菌、核盤菌屬、向日葵核盤菌、小核盤菌、同果核盤菌、尾孢菌屬、鉤絲殼屬、葡萄白粉病菌(白粉病)、叉絲單囊殼屬(白粉病)、白叉絲單囊殼、克蘭德 叉絲單囊殼、擬莖點菌屬、葡萄擬莖點黴病菌、交鏈孢菌屬、細極鏈格孢菌、蔥鏈格孢菌、交鏈格孢菌、立枯交鏈孢菌、鏈格孢菌、假單胞菌屬、丁香假單胞菌番茄變種、疫黴菌屬、致病疫黴菌、寄生疫黴菌、大豆疫黴菌、辣椒疫黴菌、樟疫黴菌、草莓疫黴菌、疫黴菌屬、櫟樹猝死病菌、棕櫚疫黴菌、菸草疫黴菌、層鏽菌屬、豆薯層鏽菌、層鏽層假尾孢菌、曲黴菌屬、黃麴黴、黑麯黴、單孢鏽菌屬、菜豆銹病菌、枝孢菌屬、多主枝孢黴、根黴菌屬、少根根黴菌、青黴菌屬、絲核菌屬、立枯絲核菌、玉蜀黍絲核菌、稻枯斑絲核菌、褐色絲核菌、禾穀絲核菌、紫紋羽絲核菌、草莓絲核菌、枝生絲核菌、覆盆子絲核菌、豆狀絲核菌、菜豆殼球孢菌、稻瘟菌、球腔菌屬、禾生球腔菌、斐濟球腔菌(黑色香蕉葉斑病)、蘋果斑點球腔菌、檸檬球腔菌、稻瘟菌屬、稻瘟病菌、鏈核盤菌屬、桃褐腐病菌、藍莓僵果病病原菌、核果鏈核盤菌、炭疽菌屬、膠孢炭疽菌、辣椒炭疽菌、石斛炭疽菌、間座殼屬、柑桔間座殼菌、棒孢菌屬、多主棒孢菌、膠鏽菌屬、植物受檜膠鏽菌、裂盾菌屬、仁果裂盾菌、黏殼孢菌屬、梨果黏殼孢菌、葡萄座腔菌屬、葡萄潰瘍病病菌、明孢盤菌屬、威爾遜孢殼菌屬、嗜果威爾遜孢殼菌、單囊殼菌屬、斑點單囊殼菌、薔薇單囊殼菌、白粉菌屬、殼多孢菌屬、穎枯殼針孢、腐黴屬、終極腐黴菌、瓜果腐黴菌、畸雌腐黴菌、簇囊腐黴菌、水生腐黴菌、森林腐黴菌、黑星菌屬、蘋果黑星病菌、輪枝孢菌屬、黑粉菌屬、裸黑粉菌、玉蜀黍黑粉菌、甘蔗鞭黑粉菌、麥角菌屬、麥角菌、腥黑粉菌屬、小麥腥黑粉菌、光滑腥黑粉菌、稻粒黑粉菌、小麥矮腥黑粉菌、莖點黴屬、大豆生莖點黴、多變莖點黴、甘藍莖點黴、禾旋孢腔菌、禾頂囊殼、炭疽菌屬、雲紋病菌屬、大麥雲紋病 菌、離蠕孢屬、長蠕孢屬、大麥長蠕孢、玉蜀黍長蠕孢、索萊長蠕孢、小麥褐斑長蠕孢或其組合。 The method of claim 37, wherein the pathogenic infection is caused by a plant fungal pathogen, a plant bacterial pathogen, a rust fungus, a Botrytis, a Botrytis cinerea, a Botrytis cinerea, an Erwinia, and a carrot soft rot Starch Amoeba, Diracium, D. dandelion, black smut, Agrobacterium, Agrobacterium tumefaciens, Xanthomonas, P. sphaeroides, Xanthomonas campestris Pathogenic species, Rhizoctonia solani, Rhizoctonia solani, Rice variety of Xanthomonas oryzae, Saccharomyces, Saccharomyces cerevisiae, Cartilian, Phloem phlei, Fusarium, Fusarium oxysporum, Wo Fusarium oxysporum, Fusarium oxysporum, Fusarium oxysporum, Fusarium oxysporum, Fusarium solani, Sclerotinia, S. sclerotiorum, Sclerotinia sclerotiorum, Sclerotinia sclerotiorum, Cercospora Genus, genus Hook, genus of powdery mildew (white powder), genus of the genus of the genus (white powder), white stalk, single sac, Cland Cross-single single-shell, Pseudomonas, Pseudomonas cepacii, Alternaria, Alternaria alternata, Alternaria alternata, Alternaria alternata, Alternaria alternata , Alternaria, Pseudomonas, Pseudomonas syringae, tomato Phytophthora, Phytophthora infestans, Phytophthora infestans, Phytophthora sojae, Phytophthora capsici, Phytophthora capsici, Phytophthora strawberry, plague Mycelium, eucalyptus, Phytophthora parasitica, Phytophthora nicotianae, rust fungus, rust fungus, rust layer pseudomonas, Aspergillus, Astragalus, Aspergillus niger, Puccinia Genus, Bean rust, Cladosporium, Cladosporium, Rhizopus, Rhizopus, Penicillium, Rhizoctonia, Rhizoctonia solani, Rhizoctonia solani, Rice radix Nuclear bacteria, Rhizoctonia solani, Rhizoctonia cerealis, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia solani, Coccidioides, Rice Phytophthora, genus genus, genus Phytophthora, Phytophthora Fiji (black banana leaf spot), Phytophthora sphaeroides, Phytophthora sinensis, Magnaporthe oryzae Rice blast fungus, Sclerotinia sclerotiorum, Peach brown rot fungus, Blueberry zombie pathogen, S. cerevisiae, Anthrax, Anthrax anthracis, Capsicum anthracis, Anthracnose, Aphis, Citrus Phytophthora, Corynebacterium, Pseudomonas aeruginosa, Puccinia, Plant rust, R. licheniformis, Pseudomonas, Schizosaccharomyces, Pear and fruit Helicobacter, grape genus, grape canker, genus, genus, genus, genus, genus, genus, genus, genus, sclerotium, sclerotium , powdery mildew, genus Polyporus, Phytophthora, Pythium, Pythium, Pythium, Pythium, Pythium, Pythium, Pythium, Black Star Phytophthora, apple black scab, Helicobacter, sphaeroides, smut, black smut, sugarcane, ergot, ergot, smut, Wheat black powder fungus, smooth black powder fungus, rice black powder fungus, wheat dwarf black powder fungus, stem mold, soybean shoot stem mold, polymorphic stem mold , Brassica napus, Trichomonas, Trichosanthes, Anthrax, Rhizoctonia, Barley Phytophthora, Helminthosporium, Helminthosporium, Helminthosporium, Helminthosporium, Helminthosporium, Helminthosporium or a combination thereof. 如請求項37之方法，其中該組合物進一步包含存在適合於使植物生長獲益及/或對易感病植物賦予保護以避免病原性感染之量之以下之一或組合：微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑或植物生長調節劑。 The method of claim 37, wherein the composition further comprises one or a combination of the presence of an amount suitable to benefit plant growth and/or to confer protection to the susceptible plant to avoid pathogenic infection: microbial, biological or chemical Insecticides, fungicides, nematicides, fungicides or plant growth regulators. 一種用於達成使植物生長獲益或對易感病植物賦予保護以避免病原性感染中之一或兩者的方法，該方法包含：輸送第一組合物與第二組合物之組合至：該植物之葉、該植物之莖皮、該植物之果實、該植物之花、該植物之種子、該植物之根部、該植物之剪枝、該植物之移植物、該植物之癒合組織；該植物周圍土壤或生長介質；在土壤或生長介質中播種該植物之種子之前的該土壤或生長介質；或在土壤或生長介質中種植該植物、該植物剪枝、該植物移植物或該植物癒合組織之前的該土壤或生長介質，該第一組合物包含以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物，呈適合於使該植物生長獲益及/或對該易感病植物賦予保護以避免病原性感染之量；且該第二組合物包含微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑、除草劑、植物提取物、植物生長調節劑或肥料之一或組合，呈適合於使該植物生長獲益及/或對該易感病植物賦予保護以避免病原性感染之量。 A method for achieving one or both of benefiting plant growth or conferring protection to a susceptible plant to avoid a pathogenic infection, the method comprising: delivering a combination of the first composition and the second composition to: a leaf of a plant, a bark of the plant, a fruit of the plant, a flower of the plant, a seed of the plant, a root of the plant, a pruning of the plant, a graft of the plant, a healing tissue of the plant; the plant a surrounding soil or growth medium; the soil or growth medium prior to sowing the seed of the plant in the soil or growth medium; or planting the plant, the plant pruning, the plant graft or the plant healing tissue in the soil or growth medium In the previous soil or growth medium, the first composition comprises a B. amyloliquefaci RTI 472 deposited with ATCC No. PTA-121166 or a biologically pure culture of a mutant having all of its identifying characteristics, suitable for making the plant Growth benefit and/or protection of the susceptible plant to avoid pathogenic infection; and the second composition comprises a microbial, biological or chemical insecticide, fungicide One or a combination of nematicides, bactericides, herbicides, plant extracts, plant growth regulators or fertilizers, suitable for benefiting the growth of the plant and/or conferring protection to the susceptible plant to avoid pathogenic infections The amount. 如請求項44之方法，其中該組合輸送至該植物之葉。 The method of claim 44, wherein the combination is delivered to the foliage of the plant. 如請求項44之方法，其中該第一組合物包含以下之一或組合：載劑、界面活性劑、分散劑或酵母提取物。 The method of claim 44, wherein the first composition comprises one or a combination of: a carrier, a surfactant, a dispersant, or a yeast extract. 如請求項44之方法，其中該植物生長益處及/或該賦予之保護係藉由以下展現：經提高之幼苗活力、經改善之根發育、經提高之植物健康、經提高之植物質量、經增加之產量、經改善之外觀、對植物病原體之經提高之抗性、經減少之病原性感染或其組合。 The method of claim 44, wherein the plant growth benefit and/or the conferred protection is manifested by improved seedling vigor, improved root development, improved plant health, enhanced plant quality, Increased yield, improved appearance, increased resistance to plant pathogens, reduced pathogenic infections, or a combination thereof. 如請求項44之方法，其中該適合於使植物生長獲益及/或賦予保護之量為約1.0×1010CFU/ha至約1.0×1014CFU/ha之解澱粉芽孢桿菌RTI472。 The method of claim 44, wherein the B. amyloliquefaci RTI 472 is adapted to benefit from plant growth and/or to confer protection in an amount from about 1.0 x 10 10 CFU/ha to about 1.0 x 10 14 CFU/ha. 如請求項44之方法，其中該解澱粉芽孢桿菌RTI472呈孢子或營養細胞形式。 The method of claim 44, wherein the B. amyloliquefaciens RTI 472 is in the form of spores or vegetative cells. 如請求項44之方法，其中該植物包含單子葉植物、雙子葉植物、穀物、玉米、甜玉米、爆玉米、玉米種、青貯玉米、飼料玉米、稻穀、小麥、大麥、高粱、蘆筍、漿果、藍莓、黑莓、樹莓、羅甘莓、越橘、蔓越橘、醋栗、接骨木、黑醋栗、蔓越莓、越莓、甘藍型蔬菜、椰菜、甘藍菜、花椰菜、芽甘藍、散葉甘藍、羽衣甘藍、芥菜、球莖甘藍、瓜類蔬菜、黃瓜、哈密瓜、甜瓜、香甘瓜、番南瓜、西瓜、南瓜、茄子、鱗莖類蔬菜、洋蔥、大蒜、蔥、陳皮、橘子、葡萄柚、檸檬、紅橘、橘柚、柚子、果蔬菜、胡椒、番茄、地面櫻桃、樹蕃茄、秋葵、葡萄、香草/香料、綠葉蔬菜、萵苣、芹菜、菠菜、香芹、菊苣、豆科植物/蔬菜(多汁且乾燥之豆子及豌豆)、豆子、四季豆、食莢菜豆、殼豆、大豆、乾豆、回回豆、利馬豆、豌豆、鷹嘴豆、豌豆瓣、扁豆、油料種子作物、芥花、蓖麻、椰子、棉花、亞麻、油棕櫚、橄欖、花生、油菜籽、紅花、芝麻、向日葵、大豆、梨果、蘋果、海棠果、梨、榅桲、夏花山楂、根/塊莖及球莖蔬菜、胡蘿蔔、馬鈴薯、甘薯、木薯、甜菜、薑、 辣根、蘿蔔、人參、蕪菁、核果、杏子、櫻桃、油桃、桃子、李子、梅子、草莓、堅果、杏仁、開心果、山核桃、核桃、榛子、板栗、腰果、山毛櫸堅果、胡桃、夏威夷果、獼猴桃、香蕉、(藍)龍舌蘭、草、草皮草、觀賞植物、一品紅、硬木剪枝、栗樹、橡樹、楓樹、甘蔗或甜菜。 The method of claim 44, wherein the plant comprises monocots, dicots, grains, corn, sweet corn, popcorn, corn, silage corn, forage corn, rice, wheat, barley, sorghum, asparagus, berries, Blueberries, blackberries, raspberries, raspberries, cranberries, cranberries, gooseberries, elderberries, currants, cranberries, berry, cabbage, broccoli, kale, broccoli, Brussels sprouts, Cabbage, kale, mustard, stalk, melon, cucumber, cantaloupe, melon, sweet melon, squash, watermelon, pumpkin, eggplant, bulbous vegetables, onions, garlic, onions, tangerine peel, oranges, grapes Pomelo, lemon, red orange, tangerine, grapefruit, fruit vegetable, pepper, tomato, ground cherry, tree tomato, okra, grape, vanilla/spice, green leafy vegetables, lettuce, celery, spinach, parsley, chicory, legume Plants/vegetables (succulent and dry beans and peas), beans, green beans, pod beans, shell beans, soybeans, dried beans, peas, lima beans, peas, chickpeas, peas, lentils, oil Sub-crops, canola, ramie, coconut, cotton, flax, oil palm, olive, peanut, rapeseed, safflower, sesame, sunflower, soybean, pear, apple, jellyfish, pear, alfalfa, summer flower haw, Roots/tubers and bulbs vegetables, carrots, potatoes, sweet potatoes, cassava, beets, ginger, Horseradish, radish, ginseng, turnip, stone fruit, apricot, cherry, nectarine, peach, plum, plum, strawberry, nut, almond, pistachio, pecan, walnut, hazelnut, chestnut, cashew, beech nut, walnut, hawaii Fruit, kiwi, banana, (blue) agave, grass, turf grass, ornamental plant, poinsettia, hardwood pruning, chestnut, oak, maple, sugar cane or beet. 如請求項50之方法，其中該植物包含大豆、豆子、食莢菜豆、小麥、棉花、玉米、胡椒、番茄、馬鈴薯、木薯、葡萄、草莓、香蕉、花生、番南瓜、南瓜、茄子或黃瓜。 The method of claim 50, wherein the plant comprises soybean, bean, pod bean, wheat, cotton, corn, pepper, tomato, potato, tapioca, grape, strawberry, banana, peanut, squash, pumpkin, eggplant or cucumber. 如請求項50之方法，其中該病原性感染由以下引起：植物真菌病原體、植物細菌病原體、鏽菌、葡萄孢屬、灰葡萄孢菌、蔥鱗葡萄孢菌、歐文菌屬、胡蘿蔔軟腐歐文菌、解澱粉歐文菌、狄克氏菌屬、達旦狄克氏菌、黑脛病菌、土壤桿菌屬、根癌土壤桿菌、黃單孢菌屬、地毯草黃單孢菌、油菜黃單孢菌胡蘿蔔致病變種、桃李黃單孢菌、核桃黃單孢菌、水稻黃單孢菌水稻變種、焦枯菌屬、葉緣焦枯菌、卡地菌屬、韌皮部桿菌、鐮刀菌屬、大刀鐮刀菌、禾穀鐮刀菌、尖孢鐮刀菌、古巴尖孢鐮刀菌、番茄尖孢鐮刀菌、棒形鐮刀菌、核盤菌屬、向日葵核盤菌、小核盤菌、同果核盤菌、尾孢菌屬、鉤絲殼屬、葡萄白粉病菌(白粉病)、叉絲單囊殼屬(白粉病)、白叉絲單囊殼、克蘭德叉絲單囊殼、擬莖點菌屬、葡萄擬莖點黴病菌、交鏈孢菌屬、細極鏈格孢菌、蔥鏈格孢菌、交鏈格孢菌、立枯交鏈孢菌、鏈格孢菌、假單胞菌屬、丁香假單胞菌番茄變種、疫黴菌屬、致病疫黴菌、寄生疫黴菌、大豆疫黴菌、辣椒疫黴菌、樟疫黴菌、草莓疫黴菌、疫黴菌屬、櫟樹猝死病菌、棕櫚疫黴菌、菸草疫黴菌、層鏽菌屬、豆薯層鏽菌、層鏽層假尾孢菌、曲黴菌屬、黃麴黴、黑麯黴、單孢鏽菌屬、菜豆銹病菌、枝孢菌屬、 多主枝孢黴、根黴菌屬、少根根黴菌、青黴菌屬、絲核菌屬、立枯絲核菌、玉蜀黍絲核菌、稻枯斑絲核菌、褐色絲核菌、禾穀絲核菌、紫紋羽絲核菌、草莓絲核菌、枝生絲核菌、覆盆子絲核菌、豆狀絲核菌、菜豆殼球孢菌、稻瘟菌、球腔菌屬、禾生球腔菌、斐濟球腔菌(黑色香蕉葉斑病)、蘋果斑點球腔菌、檸檬球腔菌、稻瘟菌屬、稻瘟病菌、鏈核盤菌屬、桃褐腐病菌、藍莓僵果病病原菌、核果鏈核盤菌、炭疽菌屬、膠孢炭疽菌、辣椒炭疽菌、石斛炭疽菌、間座殼屬、柑桔間座殼菌、棒孢菌屬、多主棒孢菌、膠鏽菌屬、植物受檜膠鏽菌、裂盾菌屬、仁果裂盾菌、黏殼孢菌屬、梨果黏殼孢菌、葡萄座腔菌屬、葡萄潰瘍病病菌、明孢盤菌屬、威爾遜孢殼菌屬、嗜果威爾遜孢殼菌、單囊殼菌屬、斑點單囊殼菌、薔薇單囊殼菌、白粉菌屬、殼多孢菌屬、穎枯殼針孢、腐黴屬、終極腐黴菌、瓜果腐黴菌、畸雌腐黴菌、簇囊腐黴菌、水生腐黴菌、森林腐黴菌、黑星菌屬、蘋果黑星病菌、輪枝孢菌屬、黑粉菌屬、裸黑粉菌、玉蜀黍黑粉菌、甘蔗鞭黑粉菌、麥角菌屬、麥角菌、腥黑粉菌屬、小麥腥黑粉菌、光滑腥黑粉菌、稻粒黑粉菌、小麥矮腥黑粉菌、莖點黴屬、大豆生莖點黴、多變莖點黴、甘藍莖點黴、禾旋孢腔菌、禾頂囊殼、炭疽菌屬、雲紋病菌屬、大麥雲紋病菌、離蠕孢屬、長蠕孢屬、大麥長蠕孢、玉蜀黍長蠕孢、索萊長蠕孢、小麥褐斑長蠕孢或其組合。 The method of claim 50, wherein the pathogenic infection is caused by a plant fungal pathogen, a plant bacterial pathogen, a rust fungus, a Botrytis, a Botrytis cinerea, a Botrytis cinerea, an Owenium genus, and a carrot soft rot Starch Amoeba, Diracium, D. dandelion, black smut, Agrobacterium, Agrobacterium tumefaciens, Xanthomonas, P. sphaeroides, Xanthomonas campestris Pathogenic species, Rhizoctonia solani, Rhizoctonia solani, Rice variety of Xanthomonas oryzae, Saccharomyces, Saccharomyces cerevisiae, Cartilian, Phloem phlei, Fusarium, Fusarium oxysporum, Wo Fusarium oxysporum, Fusarium oxysporum, Fusarium oxysporum, Fusarium oxysporum, Fusarium solani, Sclerotinia, S. sclerotiorum, Sclerotinia sclerotiorum, Sclerotinia sclerotiorum, Cercospora Genus, genus, genus, powdery mildew (white powder), genus of the genus of the genus of the genus (white powder), white stalk, single sac, clad, single sac, stalk, stalk Phomopsis solani, Alternaria, Alternaria alternata, onion chain Alternaria, Alternaria alternata, Alternaria alternata, Alternaria, Pseudomonas, Pseudomonas syringae, tomato Phytophthora, Phytophthora infestans, Phytophthora infestans, Phytophthora sojae Phytophthora capsici, Phytophthora capsici, Phytophthora infestans, Phytophthora, eucalyptus, Phytophthora palmatum, Phytophthora nicotianae, rust fungus, rust fungus, rust layer pseudomonas, koji Fungi, Astragalus, Aspergillus niger, Puccinia rot, Bean rust, Cladosporium, Cladosporium fulvum, Rhizopus, Rhizopus, Penicillium, Rhizoctonia, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia solani, Grass Nuclear bacteria, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia platensis, Rhizoctonia solani, Coccidioides, Magnaporthe oryzae, Glomus Phytophthora, Filipino bacterium (black banana leaf spot), apple spotted bacterium, lemon bacterium, rice blast, rice blast, chain genus, brown rot, blueberry fruit pathogen , Spirulina platensis, anthracnose, anthrax, anthracnose, anthracnose, anthrax, genus, citrus, genus, genus, genus, rust Genus, plant, genus, genus, genus, genus, genus, genus, genus, genus, genus, grape rot, genus Phytophthora, Phytophthora, Phytophthora, Phytophthora, Phytophthora, Rhizoctonia solani, Powdery genus, Polyporus genus, Vernacular Acupuncture, Pythium, Pythium, Pythium, Pythium, Pythium, Pythium, Pythium, Black Spot, Echinococcus, Verticillium, Black powder fungus, naked black powder fungus, maize black powder fungus, sugarcane black powder fungus, ergot fungus, ergot fungus, black fungus genus, wheat black powder fungus, smooth black powder fungus, rice grain Black powder fungus, wheat dwarf smut, stem mold, soybean stem, mold, mildew, mildew, cabbage, stem mold, Helminthosporium, grass capsule, anthracnose, moiré Phytophthora, barley genus, Helminthosporium, Helminthosporium, Helminthosporium, Helminthosporium, Helminthosporium, Schistosoma gracilis, or a combination thereof. 如請求項52之方法，其中該病原性感染由以下之一或組合引起：大豆鏽菌(豆薯層鏽菌、層鏽層假尾孢菌)且該植物包含大豆；灰葡萄孢菌(葡萄孢疫病)且該植物包含葡萄；灰葡萄孢菌(葡萄孢疫病)且該植物包含草莓； 交鏈孢菌屬(例如立枯交鏈孢菌)且該植物包含番茄；交鏈孢菌屬(例如立枯交鏈孢菌)且該植物包含馬鈴薯；豆銹病(菜豆銹病菌)且該植物包含常見豆子；白粉病菌(大豆白粉病)且該植物包含大豆；斐濟球腔菌(黑色香蕉葉斑病)或古巴尖孢鐮刀菌(巴拿馬疾病)且該植物包含香蕉；黃單孢菌屬或水稻黃單孢菌水稻變種且該植物包含稻穀；地毯草黃單孢菌且該植物包含木薯；野油菜黃單孢菌且該植物包含番茄；灰葡萄孢菌(胡椒葡萄孢疫病)且該植物包含胡椒；白粉病且該植物包含葫蘆；向日葵核盤菌(白黴)且該植物包含食莢菜豆；向日葵核盤菌(白黴)且該植物包含馬鈴薯；同果核盤菌(幣斑病)且該植物包含草皮草；南方白黴且該植物包含花生；葉斑病(尾孢菌/尾孢菌)且該植物包含花生；禾穀鐮刀菌(小麥赤黴病)且該植物包含小麥；禾生球腔菌(小麥殼針孢斑)且該植物包含小麥；穎枯殼針孢(穎枯病及殼針孢葉枯病)且該植物包含小麥；解澱粉歐文菌且該植物包含蘋果、梨及其他梨果果實；蘋果黑星病菌且該植物包含蘋果、梨及其他梨果果實；或立枯絲核菌且該植物包含小麥、稻穀、草皮草、大豆、玉米、豆科植物及蔬菜作物。 The method of claim 52, wherein the pathogenic infection is caused by one or a combination of: soybean rust (Phaeophyllum serrata, Pseudomonas serrata) and the plant comprises soybean; Botrytis cinerea (grape) Spore blight) and the plant comprises a grape; Botrytis cinerea (B. blight) and the plant comprises a strawberry; A genus of the genus Alternaria (for example, Alternaria solani) and the plant comprises a tomato; a genus of the genus Alternaria (for example, Alternaria solani) and the plant comprises a potato; soybean rust (a bean rust) and the plant Contains common beans; powdery mildew (soybean powdery mildew) and the plant contains soybeans; Fischerella filiformis (black banana leaf spot) or Fusarium oxysporum (Panama disease) and the plant contains bananas; Xanthomonas or a rice variety of Xanthomonas oryzae and the plant comprising rice; Rhizoctonia solani and the plant comprising cassava; Xanthomonas campestris and the plant comprising tomato; Botrytis cinerea (P. cinerea) and the plant Containing pepper; powdery mildew and the plant comprises a gourd; S. cerevisiae (White mold) and the plant comprises a pod bean; S. cerevisiae (White mold) and the plant comprises a potato; Sclerotinia sclerotiorum And the plant comprises turfgrass; southern white mildew and the plant comprises peanuts; leaf spot disease (C. cerevisiae / Cercospora) and the plant comprises peanuts; Fusarium graminearum (branches) and the plant comprises wheat Phytophthora (Saccharomyces cerevisiae) and Containing wheat; A. sinensis (Bacterial blight and Phytophthora capsici) and the plant comprises wheat; Amylolytic starch and the plant comprises apple, pear and other pear fruit; apple black scab and the The plant comprises apple, pear, and other pear fruit; or Rhizoctonia solani and the plant comprises wheat, rice, turf, soybean, corn, legume, and vegetable crops. 一種用於達成使植物生長獲益或對易感病植物賦予保護以避免病原性感染中之一或兩者的方法，該方法包含：將如下組合物輸送至該植物之葉、該植物之莖皮、該植物之 果實、該植物之花、該植物之種子、該植物之根部、該植物之剪枝、該植物之移植物、該植物之癒合組織；該植物周圍土壤或生長介質；在土壤或生長介質中播種該植物之種子之前的該土壤或生長介質；或在土壤或生長介質中種植該植物、該植物剪枝、該植物移植物或該植物癒合組織之前的該土壤或生長介質，該組合物包含以下各者：以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物，呈適合於使植物生長獲益及/或賦予保護以避免該病原性感染之量；及微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑、除草劑、植物提取物、植物生長調節劑或肥料之一或組合，呈適合於使該植物生長獲益及/或賦予保護以避免該病原性感染之量。 A method for achieving one or both of plant growth benefits or protection of susceptible plants to avoid pathogenic infections, the method comprising: delivering a composition to the leaves of the plant, the stem of the plant Skin, the plant Fruit, flower of the plant, seed of the plant, root of the plant, pruning of the plant, graft of the plant, healing tissue of the plant; soil or growth medium surrounding the plant; seeding in soil or growth medium The soil or growth medium prior to the seed of the plant; or the soil or growth medium prior to planting the plant, the plant pruning, the plant graft or the plant healing tissue in a soil or growth medium, the composition comprising the following Each: a biologically pure culture of Bacillus amyloliquefaciens RTI472 or a mutant having all of its identifying characteristics deposited under ATCC PTA-121166, suitable for benefiting plant growth and/or conferring protection against the pathogen An amount of a sexual infection; and one or a combination of a microbial, biological or chemical insecticide, fungicide, nematicide, bactericide, herbicide, plant extract, plant growth regulator or fertilizer, suitable for the plant Growth benefits and/or protection affords the amount of the pathogenic infection. 如請求項54之方法，其中該組合物輸送至該植物之葉。 The method of claim 54, wherein the composition is delivered to the leaves of the plant. 如請求項54之方法，其中該植物生長益處及/或為避免病原性感染所賦予之保護係藉由以下展現：經提高之幼苗活力、經改善之根發育、經提高之植物健康、經提高之植物質量、經增加之產量、經改善之外觀、對植物病原體之經提高之抗性、經減少之病原性感染或其組合。 The method of claim 54, wherein the plant growth benefit and/or the protection conferred by the avoidance of the pathogenic infection is manifested by improved seedling vigor, improved root development, improved plant health, and improved Plant quality, increased yield, improved appearance, increased resistance to plant pathogens, reduced pathogenic infection, or a combination thereof. 如請求項54之方法，其中該解澱粉芽孢桿菌RTI472呈孢子或營養細胞形式。 The method of claim 54, wherein the B. amyloliquefaciens RTI 472 is in the form of spores or vegetative cells. 如請求項54之方法，其中該植物包含單子葉植物、雙子葉植物、穀物、玉米、甜玉米、爆玉米、玉米種、青貯玉米、飼料玉米、稻穀、小麥、大麥、高粱、蘆筍、漿果、藍莓、黑莓、樹莓、羅甘莓、越橘、蔓越橘、醋栗、接骨木、黑醋栗、蔓越莓、越莓、甘藍型蔬菜、椰菜、甘藍菜、花椰菜、芽甘藍、散 葉甘藍、羽衣甘藍、芥菜、球莖甘藍、瓜類蔬菜、黃瓜、哈密瓜、甜瓜、香甘瓜、番南瓜、西瓜、南瓜、茄子、鱗莖類蔬菜、洋蔥、大蒜、蔥、陳皮、橘子、葡萄柚、檸檬、紅橘、橘柚、柚子、果蔬菜、胡椒、番茄、地面櫻桃、樹蕃茄、秋葵、葡萄、香草/香料、綠葉蔬菜、萵苣、芹菜、菠菜、香芹、菊苣、豆科植物/蔬菜(多汁且乾燥之豆子及豌豆)、豆子、四季豆、食莢菜豆、殼豆、大豆、乾豆、回回豆、利馬豆、豌豆、鷹嘴豆、豌豆瓣、扁豆、油料種子作物、芥花、蓖麻、椰子、棉花、亞麻、油棕櫚、橄欖、花生、油菜籽、紅花、芝麻、向日葵、大豆、梨果、蘋果、海棠果、梨、榅桲、夏花山楂、根/塊莖及球莖蔬菜、胡蘿蔔、馬鈴薯、甘薯、木薯、甜菜、薑、辣根、蘿蔔、人參、蕪菁、核果、杏子、櫻桃、油桃、桃子、李子、梅子、草莓、堅果、杏仁、開心果、山核桃、核桃、榛子、板栗、腰果、山毛櫸堅果、胡桃、夏威夷果、獼猴桃、香蕉、(藍)龍舌蘭、草、草皮草、觀賞植物、一品紅、硬木剪枝、栗樹、橡樹、楓樹、甘蔗或甜菜。 The method of claim 54, wherein the plant comprises monocots, dicots, grains, corn, sweet corn, popcorn, corn, silage corn, forage corn, rice, wheat, barley, sorghum, asparagus, berries, Blueberries, blackberries, raspberries, raspberries, cranberries, cranberries, gooseberries, elderberries, currants, cranberries, berry, cabbage, broccoli, kale, broccoli, Brussels sprouts, Scatter Cabbage, kale, mustard, broccoli, melon, cucumber, cantaloupe, melon, cantaloupe, squash, watermelon, pumpkin, eggplant, bulbous vegetables, onions, garlic, onions, tangerine peel, oranges, grapefruit , lemon, red tangerine, tangerine, grapefruit, fruit vegetable, pepper, tomato, ground cherry, tree tomato, okra, grape, vanilla/spice, green leafy vegetables, lettuce, celery, spinach, parsley, chicory, legumes /Vegetables (juicy and dry beans and peas), beans, green beans, food pods, shell beans, soybeans, dried beans, peas, lima beans, peas, chickpeas, peas, lentils, oilseed crops , canola, ramie, coconut, cotton, flax, oil palm, olive, peanut, rapeseed, safflower, sesame, sunflower, soybean, pear, apple, jellyfish, pear, alfalfa, summer flower hawthorn, root / Tuber and bulb vegetable, carrot, potato, sweet potato, cassava, beet, ginger, horseradish, radish, ginseng, turnip, stone fruit, apricot, cherry, nectarine, peach, plum, plum, strawberry, nut Almond, pistachio, pecan, walnut, hazelnut, chestnut, cashew, beech nut, walnut, macadamia, kiwi, banana, (blue) agave, grass, turf, ornamental, poinsettia, hardwood pruning, chestnut Trees, oaks, maples, sugar cane or beets. 如請求項54之方法，其中該植物包含大豆、豆子、食莢菜豆、小麥、棉花、玉米、胡椒、番茄、馬鈴薯、木薯、葡萄、草莓、香蕉、花生、番南瓜、南瓜、茄子或黃瓜。 The method of claim 54, wherein the plant comprises soybean, bean, pod bean, wheat, cotton, corn, pepper, tomato, potato, tapioca, grape, strawberry, banana, peanut, squash, pumpkin, eggplant or cucumber. 如請求項54之方法，其中該病原性感染由以下引起：植物真菌病原體、植物細菌病原體、鏽菌、葡萄孢屬、灰葡萄孢菌、蔥鱗葡萄孢菌、歐文菌屬、胡蘿蔔軟腐歐文菌、解澱粉歐文菌、狄克氏菌屬、達旦狄克氏菌、黑脛病菌、土壤桿菌屬、根癌土壤桿菌、黃單孢菌屬、地毯草黃單孢菌、油菜黃單孢菌胡蘿蔔致病變種、桃李黃單孢菌、核桃黃單孢菌、水稻黃單孢菌水稻變種、焦枯菌屬、葉緣焦枯菌、卡地菌屬、韌皮部桿菌、鐮刀 菌屬、大刀鐮刀菌、禾穀鐮刀菌、尖孢鐮刀菌、古巴尖孢鐮刀菌、番茄尖孢鐮刀菌、棒形鐮刀菌、核盤菌屬、向日葵核盤菌、小核盤菌、同果核盤菌、尾孢菌屬、鉤絲殼屬、葡萄白粉病菌(白粉病)、叉絲單囊殼屬(白粉病)、白叉絲單囊殼、克蘭德叉絲單囊殼、擬莖點菌屬、葡萄擬莖點黴病菌、交鏈孢菌屬、細極鏈格孢菌、蔥鏈格孢菌、交鏈格孢菌、立枯交鏈孢菌、鏈格孢菌、假單胞菌屬、丁香假單胞菌番茄變種、疫黴菌屬、致病疫黴菌、寄生疫黴菌、大豆疫黴菌、辣椒疫黴菌、樟疫黴菌、草莓疫黴菌、疫黴菌屬、櫟樹猝死病菌、棕櫚疫黴菌、菸草疫黴菌、層鏽菌屬、豆薯層鏽菌、層鏽層假尾孢菌、曲黴菌屬、黃麴黴、黑麯黴、單孢鏽菌屬、菜豆銹病菌、枝孢菌屬、多主枝孢黴、根黴菌屬、少根根黴菌、青黴菌屬、絲核菌屬、立枯絲核菌、玉蜀黍絲核菌、稻枯斑絲核菌、褐色絲核菌、禾穀絲核菌、紫紋羽絲核菌、草莓絲核菌、枝生絲核菌、覆盆子絲核菌、豆狀絲核菌、菜豆殼球孢菌、稻瘟菌、球腔菌屬、禾生球腔菌、斐濟球腔菌(黑色香蕉葉斑病)、蘋果斑點球腔菌、檸檬球腔菌、稻瘟菌屬、稻瘟病菌、鏈核盤菌屬、桃褐腐病菌、藍莓僵果病病原菌、核果鏈核盤菌、炭疽菌屬、膠孢炭疽菌、辣椒炭疽菌、石斛炭疽菌、間座殼屬、柑桔間座殼菌、棒孢菌屬、多主棒孢菌、膠鏽菌屬、植物受檜膠鏽菌、裂盾菌屬、仁果裂盾菌、黏殼孢菌屬、梨果黏殼孢菌、葡萄座腔菌屬、葡萄潰瘍病病菌、明孢盤菌屬、威爾遜孢殼菌屬、嗜果威爾遜孢殼菌、單囊殼菌屬、斑點單囊殼菌、薔薇單囊殼菌、白粉菌屬、殼多孢菌屬、穎枯殼針孢、腐黴屬、終極腐黴菌、瓜果腐黴菌、畸雌腐黴菌、簇囊腐黴菌、水生腐黴菌、森林腐黴菌、黑星菌屬、蘋果黑星病菌、輪枝孢菌屬、黑粉菌屬、裸黑粉菌、 玉蜀黍黑粉菌、甘蔗鞭黑粉菌、麥角菌屬、麥角菌、腥黑粉菌屬、小麥腥黑粉菌、光滑腥黑粉菌、稻粒黑粉菌、小麥矮腥黑粉菌、莖點黴屬、大豆生莖點黴、多變莖點黴、甘藍莖點黴、禾旋孢腔菌、禾頂囊殼、炭疽菌屬、雲紋病菌屬、大麥雲紋病菌、離蠕孢屬、長蠕孢屬、大麥長蠕孢、玉蜀黍長蠕孢、索萊長蠕孢、小麥褐斑長蠕孢或其組合。 The method of claim 54, wherein the pathogenic infection is caused by a plant fungal pathogen, a plant bacterial pathogen, a rust fungus, a Botrytis, a Botrytis cinerea, a Botrytis cinerea, an Erwinia, and a carrot soft rot Starch Amoeba, Diracium, D. dandelion, black smut, Agrobacterium, Agrobacterium tumefaciens, Xanthomonas, P. sphaeroides, Xanthomonas campestris Pathogenic species, Rhizoctonia solani, Rhizoctonia solani, rice strain of Xanthomonas oryzae, Saccharomyces, Saccharomyces cerevisiae, Carterella, Phloem bacillus, sickle Fusarium, Fusarium graminearum, Fusarium graminearum, Fusarium oxysporum, Fusarium oxysporum, Fusarium oxysporum, Fusarium oxysporum, Sclerotinia, S. cerevisiae, Sclerotinia sclerotiorum Sclerotinia sclerotiorum, Cercospora, H. genus, Powdery mildew (powdery), Trichoplusa (powder disease), White cross-shell single-shell, Clande cross-shell, Pseudomonas, Pseudomonas syringae, Pseudomonas, Alternaria alternata, Alternaria alternata, Alternaria alternata, Alternaria alternata, Alternaria, Pseudomonas, Pseudomonas syringae, tomato Phytophthora, Phytophthora infestans, Phytophthora infestans, Phytophthora sojae, Phytophthora capsici, Phytophthora infestans, Phytophthora infestans, Phytophthora, eucalyptus Phytophthora, Phytophthora palmetto, Phytophthora nicotianae, rust fungus, rust fungus, rust layer pseudomonas, Aspergillus, Astragalus, Aspergillus niger, Puccinia rot, Bean rust, Cladosporium, Cladosporium, Rhizopus, Rhizopus, Penicillium, Rhizoctonia, Rhizoctonia solani, Yusi Bacteria, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia cerealis, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia solani, Kidney bean Coccidioides, Magnaporthe oryzae, Glomus, G. sphaeroides, Fischer's genus Filipino (black banana leaf spot), P. sphaeroides, Trichoderma lucidum, Magnaporthe oryzae, Magnaporthe oryzae , Sclerotinia genus, brown rot fungus, blueberry fruit disease pathogen, S. cerevisiae, anthracnose, anthrax, anthracnose, anthracnose, anthraquinone, citrus Phytophthora, Corynebacterium, Pseudomonas aeruginosa, Puccinia, Phytophthora rust, Phytophthora, Phytophthora, Phytophthora, Phaeocystis, Grape genus, grape canker, Phytophthora, Wilson genus, Phytophthora, Phytophthora, Phytophthora, Phytophthora capsici, powdery mildew Genus, genus Pseudomonas, Phytophthora, Pythium, Pythium, Pythium, Pythium, Pythium, Pythium, Lin Pythium, Venturia, inaequalis, round Cladosporium, Ustilago, nude Ustilago, Zea mays, black sugar stalk, ergot, ergot, smut, wheat smut, smooth smut, rice black powder, wheat dwarf smut , Stem genus, soybean stem, mold, polymorphic stem mold, cabbage stem mold, Helminthosporium, Hessian shell, Anthrax, Rhizoctonia, Barley, and Mosquito Sporozoites, Helminthosporium, Helminthosporium longissima, Helminthosporium fulvus, Helminthosporium serrata, Helminthosporium fulvum or a combination thereof. 如請求項60之方法，其中該病原性感染由以下之一或組合引起：大豆鏽菌(豆薯層鏽菌、層鏽層假尾孢菌)且該植物包含大豆；灰葡萄孢菌(葡萄孢疫病)且該植物包含葡萄；灰葡萄孢菌(葡萄孢疫病)且該植物包含草莓；交鏈孢菌屬(例如立枯交鏈孢菌)且該植物包含番茄；交鏈孢菌屬(例如立枯交鏈孢菌)且該植物包含馬鈴薯；豆銹病(菜豆銹病菌)且該植物包含常見豆子；白粉病菌(大豆白粉病)且該植物包含大豆；斐濟球腔菌(黑色香蕉葉斑病)或古巴尖孢鐮刀菌(巴拿馬疾病)且該植物包含香蕉；黃單孢菌屬或水稻黃單孢菌水稻變種且該植物包含稻穀；地毯草黃單孢菌且該植物包含木薯；野油菜黃單孢菌且該植物包含番茄；灰葡萄孢菌(胡椒葡萄孢疫病)且該植物包含胡椒；白粉病且該植物包含葫蘆；向日葵核盤菌(白黴)且該植物包含食莢菜豆；向日葵核盤菌(白黴)且該植物包含馬鈴薯；同果核盤菌(幣斑病)且該植物包含草皮草；南方白黴且該植物包含花生； 葉斑病(尾孢菌/尾孢菌)且該植物包含花生；禾穀鐮刀菌(小麥赤黴病)且該植物包含小麥；禾生球腔菌(小麥殼針孢斑)且該植物包含小麥；穎枯殼針孢(穎枯病及殼針孢葉枯病)且該植物包含小麥；解澱粉歐文菌且該植物包含蘋果、梨及其他梨果果實；蘋果黑星病菌且該植物包含蘋果、梨及其他梨果果實；或立枯絲核菌且該植物包含小麥、稻穀、草皮草、大豆、玉米、豆科植物及蔬菜作物。 The method of claim 60, wherein the pathogenic infection is caused by one or a combination of: soybean rust (Phaeophyllum serrata, Pseudomonas sphaeroides) and the plant comprises soybean; Botrytis cinerea (grape Spore plague) and the plant comprises a grape; Botrytis cinerea (B. blight) and the plant comprises a strawberry; a genus Alternaria (eg, Alternaria solani) and the plant comprises a tomato; For example, Alternaria solani) and the plant comprises potato; bean rust (String rust) and the plant contains common beans; powdery mildew (soybean powdery mildew) and the plant contains soybean; Ficus sclerotium (black banana leaf spot) Disease) or Fusarium oxysporum (Panama disease) and the plant comprises banana; Xanthomonas or Xanthomonas oryzae rice variety and the plant comprises rice; Trichophyton rubrum and the plant contains cassava; Xanthomonas camphora and the plant comprises tomato; Botrytis cinerea (P. cinerea) and the plant comprises pepper; powdery mildew and the plant comprises a gourd; S. cerevisiae (White mold) and the plant comprises a pod bean Sunflower Sclerotium (White mold) The plant comprises potato; pyrenes same sclerotiorum (dollar spot) and the turf grass plant comprises; South mildew and the peanut plant comprises; Leaf spot (C. cerevisiae / Cercospora) and the plant comprises peanuts; Fusarium graminearum (branches of wheat) and the plant comprises wheat; Phytophthora sinensis (S. cerevisiae) and the plant comprises wheat ; Phyllostachys pubescens (Bacterial blight and Phytophthora capsici) and the plant comprises wheat; Amylolytic starch and the plant comprises apple, pear and other pear fruit; apple black scab and the plant comprises apple , pears and other pear fruit; or Rhizoctonia solani and the plant comprises wheat, rice, turf, soybean, corn, legume and vegetable crops. 如請求項54之方法，其中該適合於使植物生長獲益及/或賦予保護以避免病原性感染之量為約1.0×1010CFU/ha至約1.0×1014CFU/ha之解澱粉芽孢桿菌RTI472。 The method of claim 54, wherein the amylose spore is suitable for benefiting plant growth and/or conferring protection against pathogenic infection in an amount of from about 1.0 x 10 10 CFU/ha to about 1.0 x 10 14 CFU/ha. Bacillus RTI472. 一種組合物，其包含以下中之至少一者：分離之豐原素-MA化合物、分離之豐原素MB化合物、分離之豐原素MC化合物、分離之去羥基豐原素MA化合物、分離之去羥基豐原素MB化合物、分離之豐原素H化合物、分離之豐原素I化合物及分離之去羥基豐原素I化合物，呈適合於對易感病植物賦予保護以避免病原性感染之量，該化合物具有下式： 其中R為OH，n在8至20範圍內，FA為直鏈、異或反異，且：對於豐原素MA，X1為Ala，X2為Thr，且X3為Met；對於豐原素MB，X1為Val，X2為Thr，且X3為Met；對於豐原素MC，X1為 Aba，X2為Thr，且X3為Met；對於豐原素H，X1為Val，X2為Thr，且X3為Hcy；且對於豐原素I，X1為Ile，X2為Thr，且X3為Ile；且其中R為H，n在8至20範圍內，FA為直鏈、異或反異，且：對於去羥基豐原素MA，X1為Ala，X2為Thr，且X3為Met；對於去羥基豐原素MB，X1為Val，X2為Thr，且X3為Met；且對於去羥基豐原素I，X1為Ile，X2為Thr，且X3為Ile。 A composition comprising at least one of the following: an isolated abundance-MA compound, an isolated abundance MB compound, an isolated abundance MC compound, an isolated dehydroxyl-protonin MA compound, an isolated dehydroxyeductin The MB compound, the isolated abundance H compound, the isolated abundance I compound, and the isolated hydroxy-hydroxytoxin I compound are in an amount suitable for imparting protection to a susceptible plant to avoid a pathogenic infection. The compound has the formula: Wherein R is OH, n is in the range of 8 to 20, FA is linear, XOR or di-isomeric, and: for abundance MA, X 1 is Ala, X 2 is Thr, and X 3 is Met; for abundance MB X 1 is Val, X 2 is Thr, and X 3 is Met; for abundance MC, X 1 is Aba, X 2 is Thr, and X 3 is Met; for abundance H, X 1 is Val, X 2 Is Thr and X 3 is Hcy; and for abundance I, X 1 is Ile, X 2 is Thr, and X 3 is Ile; and wherein R is H, n is in the range of 8 to 20, and FA is linear, XOR or singular, and: for dehydroxylectin MA, X 1 is Ala, X 2 is Thr, and X 3 is Met; for dehydroxyl-rich MB, X 1 is Val, X 2 is Thr, and X 3 Is Met; and for dehydroxylectin I, X 1 is Ile, X 2 is Thr, and X 3 is Ile. 如請求項63之組合物，其進一步包含存在適合於使植物生長獲益及/或對該易感病植物賦予保護以避免病原性感染之量之以下之一或組合：微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑、除草劑、植物提取物、植物生長調節劑或肥料。 The composition of claim 63, further comprising one or a combination of the presence of an amount suitable for benefiting plant growth and/or conferring protection to the susceptible plant to avoid pathogenic infection: microbial, biological or chemical killing Insecticides, fungicides, nematicides, fungicides, herbicides, plant extracts, plant growth regulators or fertilizers. 如請求項64之組合物，其中該殺真菌劑係選自由氟吡菌醯胺加上得克利(tebuconazole)、四氯異苯腈(chlorothalonil)、甲基托布津(thiophanate-methyl)、丙硫菌唑(prothioconazole)及氫氧化銅組成之群。 The composition of claim 64, wherein the fungicide is selected from the group consisting of flupirtine plus tebuconazole, chlorothalonil, thiophanate-methyl, and propyl sulfide. A group consisting of prothioconazole and copper hydroxide. 如請求項63之組合物，其中該組合物呈液體、粉塵、可撒佈顆粒或乾燥可濕潤顆粒形式。 The composition of claim 63, wherein the composition is in the form of a liquid, dust, sprinkable particles or dry wettable particles. 一種組合物，其包含適合於對易感病植物賦予保護以避免病原性感染之量之以下之一或兩者：分子量等於3341.6Da之分離之Ericin S化合物，及分子量等於2985.4Da之分離之Ericin A化合物。 A composition comprising one or both of the following: an Ericin S compound having a molecular weight equal to 3341.6 Da, and an isolated Ericin having a molecular weight equal to 2985.4 Da, which is suitable for imparting protection to a susceptible plant to avoid pathogenic infection. Compound A. 如請求項67之組合物，其進一步包含存在適合於使植物生長獲益及/或對該易感病植物賦予保護以避免病原性感染之量之以下之一或組合：微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑、除草劑、植物提取物、植物生長調節劑或肥料。 The composition of claim 67, further comprising one or a combination of the presence of a quantity suitable for benefiting plant growth and/or conferring protection to the susceptible plant to avoid pathogenic infection: microbial, biological or chemical killing Insecticides, fungicides, nematicides, fungicides, herbicides, plant extracts, plant growth regulators or fertilizers. 如請求項68之組合物，其中該殺真菌劑為以下之一或多者：苯 并烯氟菌唑(benzovindiflupyr)、抗霜黴菌(anitiperonosporic)、辛唑嘧菌胺(ametoctradin)、吲唑磺菌胺(amisulbrom)、銅鹽(包括氫氧化銅、氯氧化銅、硫酸銅及過氧硫酸銅)、白可列(boscalid)、硫氟醯胺(thiflumazide)、氟替尼(flutianil)、呋霜靈(furalaxyl)、腐絕(thiabendazole)、麥鏽靈(benodanil)、滅普寧(mepronil)、艾索非他米(isofetamid)、甲呋醯胺(fenfuram)、必殺吩(bixafen)、氟唑菌醯胺(fluxapyroxad)、噴福芬(penflufen)、氟唑環菌胺(sedaxane)、丁香菌酯(coumoxystrobin)、烯肟菌酯(enoxastrobin)、氟菌蟎酯(flufenoxystrobin)、唑菌酯(pyraoxystrobin)、唑胺菌酯(pyrametostrobin)、三環吡菌威(triclopyricarb)、烯肟菌胺(fenaminstrobin)、苯氧菌胺(metominostrobin)、吡菌苯威(pyribencarb)、敵蟎普(meptyldinocap)、三苯醋錫(fentin acetate)、氯化三苯錫(fentin chloride)、氫氧化三苯錫(fentin hydroxide)、土黴素(oxytetracycline)、克氯得(chlozolinate)、地茂散(chloroneb)、四氯硝基苯(tecnazene)、依得利(etridiazole)、霜黴威(iodocarb)、硫菌威(prothiocarb)、枯草芽孢桿菌合成、解澱粉芽孢桿菌(包括菌株QST 713、FZB24、MBI600及D747)、來自互生葉白千層(Melaleuca alternifolia)之提取物、來自白羽扇豆(Lupinus albus doce)之提取物、BLAD多肽、啶菌噁唑(pyrisoxazole)、噁咪唑(oxpoconazole)、乙環唑(etaconazole)、胺苯吡菌酮(fenpyrazamine)、萘替芬(naftifine)、特比萘芬(terbinafine)、維利微素(validamycin)、丁吡嗎啉(pyrimorph)、威利芬那雷特(valifenalate)、熱必斯(fthalide)、撲殺熱(probenazole)、異噻菌胺(isotianil)、昆布糖(laminarin)、來自大虎杖之提取物、亞磷酸及鹽、葉枯酞(teclofthalam)、咪唑嗪(triazoxide)、甲氧苯唳菌 (pyriofenone)、有機油、碳酸氫鉀、四氯異苯腈、唑呋草(fluoroimide)；唑類，包括比多農(bitertanol)、糠菌唑(bromuconazole)、環克座(cyproconazole)、待克利(difenoconazole)、達克利(diniconazole)、恩康唑(enilconazole)、依普座(epoxiconazole)、氟喹唑(fluquinconazole)、芬克座(fenbuconazole)、護矽得(flusilazole)、護汰芬(flutriafol)、菲克利(hexaconazole)、亞胺唑(imibenconazole)、種菌唑(ipconazole)、滅特座(metconazole)、邁克尼(myclobutanil)、平克座(penconazole)、普克利(propiconazole)、丙硫菌唑(prothioconazole)、矽氟唑(simeconazole)、三泰芬(triadimefon)、三泰隆(triadimenol)、得克利、四克利(tetraconazole)、滅菌唑(triticonazole)、咪醯胺(prochloraz)、披扶座(pefurazoate)、依滅列(imazalil)、賽福座(triflumizole)、賽座滅(cyazofamid)、免賴得(benomyl)、貝芬替(carbendazim)、腐絕(thia-bendazole)、麥穗靈(fuberidazole)、噻唑菌胺(ethaboxam)、依得利(etridiazole)及殺紋寧(hymexazole)、氧環唑(azaconazole)、達克利-M(diniconazole-M)、噁咪唑(oxpoconazol)、巴克素(paclobutrazol)、烯效唑(uniconazol)、1-(4-氯-苯基)-2-([1,2,4]***-1-基)-環庚醇及硫酸依滅列(imazalilsulfphate)；嗜毬果傘素，包括亞托敏(azoxystrobin)、醚菌胺(dimoxystrobin)、烯肟菌酯(enestroburin)、氟嘧菌酯(fluoxastrobin)、克收欣(kresoxim-methyl)、苯氧菌胺(methominostrobin)、肟醚菌胺(orysastrobin)、啶氧菌酯(picoxystrobin)、百克敏(pyraclostrobin)、三氟敏(trifloxystrobin)、烯肟菌酯(enestroburin)、(2-氯-5-[1-(3-甲基苄氧亞胺基)乙基]苄基)胺基甲酸甲酯、(2-氯-5-[1-(6-甲基吡啶-2-基甲氧亞胺基)乙基]苄基)胺 基甲酸甲酯及2-(鄰-(2,5-二甲基苯氧亞甲基)-苯基)-3-甲氧基丙烯酸甲酯、2-(2-(6-(3-氯-2-甲基-苯氧基)-5-氟-嘧啶-4-基氧基)-苯基)-2-甲氧亞胺基-N-甲基-乙醯胺及3-甲氧基-2-(2-(N-(4-甲氧基-苯基)-環丙烷伸亞胺醯基硫基甲基)-苯基)-丙烯酸甲酯；羧醯胺，包括萎鏽靈(carboxin)、本達樂(benalaxyl)、本達樂-M、環醯菌胺(fenhexamid)、福多寧(flutolanil)、福拉比(furametpyr)、滅普寧(mepronil)、滅達樂(metalaxyl)、右滅達樂(mefenoxam)、呋醯胺(ofurace)、歐殺斯(oxadixyl)、嘉保信(oxycarboxin)、吡噻菌胺(penthiopyrad)、吡唑萘菌胺(isopyrazam)、賽氟滅(thifluzamide)、汰敵寧(tiadinil)、3,4-二氯-N-(2-氰基苯基)異噻唑-5-甲醯胺、達滅芬(dimethomorph)、氟嗎啉(flumorph)、氟醯菌胺(flumetover)、氟吡菌胺(fluopicolide)(吡考苯紮(picobenzamid))、座賽胺(zoxamide)、加普胺(carpropamid)、二氯西莫(diclocymet)、雙炔醯菌胺(mandipropamid)、N-(2-(4-[3-(4-氯苯基)丙-2-炔氧基]-3-甲氧苯基)乙基)-2-甲磺醯基-胺基-3-甲基丁醯胺、N-(2-(4-[3-(4-氯-苯基)丙-2-炔氧基]-3-甲氧基-苯基)乙基)-2-乙磺醯胺基-3-甲基丁醯胺、3-(4-氯苯基)-3-(2-異丙氧羰基-胺基-3-甲基-丁醯胺基)丙酸甲酯、N-(4'-溴聯苯-2-基)-4-二氟甲基^-甲基噻唑-δ-甲醯胺、N-(4'-三氟甲基-聯苯-2-基)-4-二氟甲基-2-甲基噻唑-5-甲醯胺、N-(4'-氯-3'-氟聯苯-2-基)-4-二氟甲基-2-甲基-噻唑-5-甲醯胺、N-(3\4'-二氯-4-氟聯苯-2-基)-3-二氟-甲基-1-甲基-吡唑-4-甲醯胺、N-(3',4'-二氯-5-氟聯苯-2-基)-3-二氟甲基-1-甲基吡唑-4-甲醯胺、N-(2-氰基-苯基)-3,4-二氯異噻唑-5-甲醯胺、2-胺基-4-甲基-噻唑-5-甲醯苯胺、2-氯-N-(1,1,3-三甲基-茚滿-4-基)-菸鹼醯胺、N-(2-(1,3-二甲基丁基)-苯基)-1,3-二甲基-5-氟-1H-吡唑-4-甲醯胺、N-(4'-氯-3',5-二氟-聯苯-2-基)-3-二氟甲 基-1-甲基-1H-吡唑-4-甲醯胺、N-(4'-氯-3',5-二氟-聯苯-2-基)-3-三氟甲基-1-甲基-1H-吡唑-4-甲醯胺、N-(3',4'-二氯-5-氟-聯苯-2-基)-3-三氟甲基-1-甲基-1H-吡唑-4-甲醯胺、N-(3',5-二氟-4'-甲基-聯苯-2-基)-3-二氟甲基-1-甲基-1H-吡唑-4-甲醯胺、N-(3',5-二氟-4'-甲基-聯苯-2-基)-3-三氟甲基-1-甲基-1H-吡唑-4-甲醯胺、N-(順-2-雙環丙基-2-基-苯基)-3-二氟甲基-1-甲基-1H-吡唑-4-甲醯胺、N-(反-2-雙環丙基-2-基-苯基)-3-二氟-甲基-1-甲基-1H-吡唑-4-甲醯胺、氟吡菌醯胺、N-(3-乙基-3,5-5-三甲基-環己基)-3-甲醯胺基-2-羥基-苯甲醯胺、土黴素、矽硫芬、N-(6-甲氧基-吡啶-3-基)環丙烷甲醯胺、2-碘-N-苯基-苯甲醯胺、N-(2-雙環-丙基-2-基-苯基)-3-二氟甲基-1-甲基吡唑-4-基甲醯胺、N-(3',4',5'-三氟聯苯-2-基)-1,3-二甲基吡唑-4-基甲醯胺、N-(3',4',5'-三氟聯苯-2-基)-1,3-二甲基-5-氟吡唑-4-基-甲醯胺、N-(3',4',5'-三氟聯苯-2-基)-5-氯-1,3-二甲基-吡唑-4-基甲醯胺、N-(3',4',5'-三氟聯苯-2-基)-3-氟甲基-1-甲基吡唑-4-基甲醯胺、N-(3',4',5'-三氟聯苯-2-基)-3-(氯氟甲基)-1-甲基吡唑-4-基甲醯胺、N-(3',4',5'-三氟聯苯-2-基)-3-二氟甲基-1-甲基吡唑-4-基甲醯胺、N-(3',4',5'-三氟聯苯-2-基)-3-二氟甲基-5-氟-1-甲基吡唑-4-基甲醯胺、N-(3',4',5'-三氟聯苯-2-基)-5-氯-3-二氟甲基-1-甲基吡唑-4-基甲醯胺、N-(3',4',5'-三氟聯苯-2-基)-3-(氯二氟甲基)-1-甲基吡唑-4-基甲醯胺、N-(3',4',5'-三氟聯苯-2-基)-1-甲基-3-三氟甲基吡唑-4-基甲醯胺、N-(3',4',5'-三氟聯苯-2-基)-5-氟-1-甲基-3-三氟甲基吡唑-4-基甲醯胺、N-(3',4',5'-三氟聯苯-2-基)-5-氯-1-甲基-3-三氟甲基吡唑-4-基甲醯胺、N-(2',4',5'-三氟聯苯-2-基)-1,3-二甲基吡唑-4-基甲醯胺、N-(2',4',5'-三氟聯苯-2-基)-1,3-二甲基-5-氟吡唑-4-基甲醯胺、N-(2',4',5'-三氟聯苯-2-基)-5-氯-1,3-二甲基吡唑-4-基甲醯胺、N- (2',4',5'-三氟聯苯-2-基)-3-氟甲基-1-甲基吡唑-4-基甲醯胺、N-(2',4',5'-三氟聯苯-2-基)-3-(氯氟甲基)-1-甲基吡唑-4-基甲醯胺、N-(2',4',5'-三氟聯苯-2-基)-3-二氟甲基-1-甲基吡唑-4-基甲醯胺、N-(2',4',5'-三氟聯苯-2-基)-3-二氟甲基-5-氟-1-甲基吡唑-4-基甲醯胺、N-(2',4',5'-三氟聯苯-2-基)-5-氯-3-二氟甲基-1-甲基吡唑-4-基甲醯胺、N-(2',4',5'-三氟聯苯-2-基)-3-(氯二氟甲基)-1-甲基吡唑-4-基甲醯胺、N-(2',4',5'-三氟聯苯-2-基)-1-甲基-3-三氟甲基吡唑-4-基甲醯胺、N-(2',4',5'-三氟聯苯-2-基)-5-氟-1-甲基-3-三氟甲基吡唑-4-基甲醯胺、N-(2',4',5'-三氟聯苯-2-基)-5-氯-1-甲基-3-三氟甲基吡唑-4-基甲醯胺、N-(3',4'-二氯-3-氟聯苯-2-基)-1-甲基-3-三氟甲基-1H-吡唑-4-甲醯胺、N-(3',4'-二氯-3-氟聯苯-2-基)-1-甲基-3-二氟甲基-1H-吡唑-4-甲醯胺、N-(3',4'-二氟-3-氟聯苯-2-基)-1-甲基-3-三氟甲基-1H-吡唑-4-甲醯胺、N-(3',4'-二氟-3-氟聯苯-2-基)-1-甲基-S-二氟甲基-1H-吡唑-4-甲醯胺、N-(3'-氯-4'-氟-3-氟聯苯-2-基)-1-甲基-3-二氟甲基-1H-吡唑-4-甲醯胺、N-(3',4'-二氯-4-氟聯苯-2-基)-1-甲基-3-三氟甲基-1H-吡唑-4-甲醯胺、N-(3',4'-二氟-4-氟聯苯-2-基)-1-甲基-S-三氟甲基-1H-吡唑-4-甲醯胺、N-(3',4'-二氯-4-氟聯苯-2-基)-1-甲基-3-二氟甲基-1H-吡唑-4-甲醯胺、N-(3',4'-二氟-4-氟聯苯-2-基)-1-甲基-3-二氟甲基-1H-吡唑-4-甲醯胺、N-(3'-氯-4'-氟-4-氟聯苯-2-基)-1-甲基-S-二氟甲基-1H-吡唑-4-甲醯胺、N-(3',4'-二氯-5-氟聯苯-2-基)-1-甲基-3-三氟甲基-1H-吡唑-4-甲醯胺、N-(3',4'-二氟-5-氟聯苯-2-基)-1-甲基-3-三氟甲基-1H-吡唑-4-甲醯胺、N-(3',4'-二氯-5-氟聯苯-2-基)-1-甲基-S-二氟甲基-1H-吡唑-甲醯胺、N-(3',4'-二氟-5-氟聯苯-2-基)-1-甲基-3-二氟甲基-1H-吡唑-4-甲醯胺、N-(3',4'-二氯-5-氟聯苯-2-基)-1,3-二甲基-1H-吡唑-4-甲醯胺、N-(3'-氯-4'-氟-5-氟聯苯-2-基)-1- 甲基-3-二氟甲基-1H-吡唑-4-甲醯胺、N-(4'-氟-4-氟聯苯-2-基)-1-甲基-3-三氟甲基-1H-吡唑-4-甲醯胺、N-(4'-氟-5-氟聯苯-2-基)-1-甲基-3-三氟甲基-1H-吡唑-4-甲醯胺、N-(4'-氯-5-氟聯苯-2-基)-1-甲基-3-三氟甲基-1H-吡唑-4-甲醯胺、N-(4'-甲基-5-氟聯苯-2-基)-1-甲基-3-三氟甲基-1H-吡唑-4-甲醯胺、N-(4'-氟-5-氟聯苯-2-基)-1,3-二甲基-1H-吡唑-4-甲醯胺、N-(4'-氯-5-氟聯苯-2-基)-1,3-二甲基-1H-吡唑-4-甲醯胺、N-(4'-甲基-5-氟聯苯-2-基)-1,3-二甲基-1H-吡唑-4-甲醯胺、N-(4'-氟-6-氟聯苯-2-基)-1-甲基-3-三氟甲基-1H-吡唑-4-甲醯胺、N-(4'-氯-6-氟聯苯-2-基)-1-甲基-3-三氟甲基-1H-吡唑-4-甲醯胺、N-[2-(1,1,2,3,3,3-六氟丙氧基)-苯基]-3-二氟甲基-1-甲基-1H-吡唑-4-甲醯胺、N-[4'-(三氟甲硫基)-聯苯-2-基]-3-二氟甲基-1-甲基-1H-吡唑-4-甲醯胺及N-[4'-(三氟甲硫基)-聯苯-2-基]-1-甲基-3-三氟甲基-1-甲基-1H-吡唑-4-甲醯胺；雜環化合物，包括扶吉胺(fluazinam)、比芬諾(pyrifenox)、布瑞莫(bupirimate)、嘧菌環胺(cyprodinil)、芬瑞莫(fenarimol)、富米綜(ferimzone)、嘧菌胺(mepanipyrim)、尼瑞莫(nuarimol)、派美尼(pyrimethanil)、賽福寧(triforine)、拌種咯(fenpiclonil)、護汰寧(fludioxonil)、阿迪嗎啉(aldimorph)、嗎菌靈(dodemorph)、芬普福(fenpropimorph)、三得芬(tridemorph)、苯鏽啶(fenpropidin)、依普同(iprodione)、撲滅寧(procymidone)、免克寧(vinclozolin)、凡殺同(famoxadone)、咪唑菌酮(fenamidone)、辛噻酮(octhilinone)、撲殺熱(proben-azole)、5-氯-7-(4-甲基-哌啶-1-基)-6-(2,4,6-三氟苯基)-[1,2,4]***并[1,5-a]嘧啶、敵菌靈(anilazine)、達滅淨(diclomezine)、百快隆(pyroquilon)、丙氧喹啉(proquinazid)、三賽唑(tricyclazole)、2-丁氧基-6-碘-3-丙基烯-4-酮、酸化苯并噻二唑-S-甲酯、四氯丹(captafol)、蓋普丹 (captan)、邁隆(dazomet)、福爾培(folpet)、氰菌胺(fenoxanil)、快諾芬(quinoxyfen)、N,N-二甲基-3-(3-溴-6-氟-2-甲基吲哚-1-磺醯基)-[1,2,4]***-1-磺醯胺、5-乙基-6-辛基-[1,2,4]***并[1,5-a]嘧啶-2,7-二胺、2,3,5,6-四氯-4-甲磺醯基-吡啶、3,4,5-三氯-吡啶-2,6-二甲腈、N-(1-(5-溴-3-氯-吡啶-2-基)-乙基)-2,4-二氯-菸鹼醯胺、N-((5-溴-3-氯吡啶-2-基)-甲基)-2,4-二氯-菸鹼醯胺、二氟林(diflumetorim)、氯啶(nitrapyrin)、嗎菌靈乙酸酯(dodemorphacetate)、唑呋草(fluoroimid)、殺稻瘟菌素-S(blasticidin-S)、蟎離丹(chinomethionat)、咪菌威(debacarb)、野燕枯(difenzoquat)、野燕枯-甲基硫酸鹽(difenzoquat-methylsulphat)、歐索林酸(oxolinic acid)及粉病靈(piperalin)；胺基甲酸酯，包括鋅錳乃浦(mancozeb)、錳乃浦(maneb)、威百畝(metam)、磺菌威(methasulphocarb)、免得爛(metiram)、福美鐵(ferbam)、甲基鋅乃浦(propineb)、得恩地(thiram)、鋅乃浦(zineb)、益穗(ziram)、乙黴威(diethofencarb)、纈黴威(iprovalicarb)、苯噻菌胺(benthiavalicarb)、霜黴威(propamocarb)、霜黴威鹽酸鹽、N-(1-(1-(4-氰基苯基)-乙磺醯基)丁-2-基)胺基甲酸4-氟苯酯、3-(4-氯-苯基)-3-(2-異丙氧基羰胺基-3-甲基-丁醯基胺基)丙酸甲酯；及其他殺真菌劑，包括胍、多寧(dodine)、多寧游離鹼、克熱淨(iminoctadine)、雙胍鹽(guazatine)；抗生素：春日黴素(kasugamycin)、土黴素及其鹽、鏈黴素(streptomycin)、保粒黴素(polyoxin)、維利黴素A(validamycin A)；硝基苯基衍生物：百蟎克(binapacryl)、白粉克(dinocap)、大脫蟎(dinobuton)；含硫雜環基化合物：腈硫醌(dithianon)、稻瘟靈(isoprothiolane)；有機金屬化合物：薯瘟錫鹽；有機磷化合物：護粒松(edifenphos)、丙基喜樂松 (iprobenfos)、福賽得(fosetyl)、福賽得-鋁、亞磷酸及其鹽、白粉松(pyrazophos)、甲基脫克松(tolclofos-methyl)；有機氯化合物：益發靈(dichlofluanid)、氟硫滅(flusulfamide)、六氯苯、苯酞(phthalide)、賓克隆(pencycuron)、五氯硝基苯(quintozene)、多保淨(thiophanate)、甲基多保淨、甲基益發靈(tolylfluanid)；其他：環氟菌胺(cyflufenamid)、克絕(cymoxanil)、二甲嘧酚(dimethirimol)、依瑞莫(ethirimol)、呋霜靈(furalaxyl)、滅芬農(metrafenone)及螺環菌胺(spiroxamine)、雙胍辛乙酸鹽、克熱淨乙酸鹽(iminoc-tadine-triacetate)、克熱淨-參(烷苯磺酸鹽)(iminoctadine-tris(albesilate))、春日黴素鹽酸鹽水合物、二氯酚(dichlorophen)、五氯苯酚(pentachlorophenol)及其鹽、N-(4-氯-2-硝基-苯基)-N-乙基-4-甲基-苯磺醯胺、氯硝胺(dicloran)、異丙基滅鏽胺(nitrothal-isopropyl)、四氯硝基苯(tecnazen)、聯苯(biphenyl)、溴硝丙二醇(bronopol)、二苯胺、米多黴素(mildiomycin)、啉銅(oxincopper)、調環酸鈣(prohexadione calcium)、N-(環丙基甲氧基亞胺基-(6-二氟甲氧基-2,3-二氟-苯基)-甲基)-2-苯基乙醯胺、N'-(4-(4-氯-3-三氟甲基-苯氧基)-2,5-二甲基-苯基)-N-乙基-N-甲基甲脒、N'-(4-(4-氟-3-三氟甲基-苯氧基)-2,5-二甲基-苯基)-N-乙基-N-甲基甲脒、N'-(2-甲基-5-三氟甲基-4-(3-三甲基矽烷基-丙氧基)-苯基)-N-乙基-N-甲基甲脒及N'-(5-二氟甲基-2-甲基-4-(3-三甲基矽烷基-丙氧基)-苯基)-N-乙基-N-甲基甲脒。 The composition of claim 68, wherein the fungicide is one or more of the following: benzovindiflupyr, anitiperonosporic, ametoctradin, oxazolidine Amisulbrom, copper salts (including copper hydroxide, copper oxychloride, copper sulfate and copper peroxosulfate), boscalid, thiflumazide, flutianil, fur Furalaxyl, thiabendazole, benodanil, mepronil, isofetamid, fenfuram, bixafen, fluazole Fluxapyroxad, penflufen, sedaxane, coumoxystrobin, enoxastrobin, flufenoxystrobin, pyraclostrobin Pyraoxystrobin), pyramitostrobin, triclopyricarb, fenaminstrobin, metominostrobin, pyribencarb, meptyldinocap , fentin acetate, fentin chloride, triphenyltin hydroxide (fentin hydroxide), oxytetracycline, chlozolinate, chloroneb, tecnazene, etridiazole, iodocarb, sulfur Prothiocarb, Bacillus subtilis synthesis, Bacillus amyloliquefaciens (including strains QST 713, FZB24, MBI600 and D747), extract from Melaleuca alternifolia , from white lupins ( Lupinus albus doce) Extract, BLAD polypeptide, pyrisoxazole, oxpoconazole, etaconazole, fenpyrazamine, naftifine, terbinafine Terbinafine), validamycin, pyrimorph, valifenalate, fthalide, probenazole, isotianil, Laminarin, extract from Polygonum cuspidatum, phosphorous acid and salt, teclofthalam, triazoxide, pyriofenone, organic oil, potassium bicarbonate, tetrachloroethylene Benzotrile, fluoroimide; azoles, including more than (bitertanol), bromuconazole, cyproconazole, difenoconazole, diniconazole, enilconazole, epoxiconazole, fluquinconazole , fenbuconazole, flusilazole, flutriafol, hexaconazole, imibenconazole, ipconazole, metconazole, micney (myclobutanil), penconazole, propiconazole, prothioconazole, simeconazole, triadimefon, triadimenol, texley, tetrakeley (tetraconazole), triticonazole, prochloraz, pefurazoate, imazalil, triflumizole, cyazofamid, benomyl ), carbendazim, thia-bendazole, fuberidazole, ethaboxam, etridiazole and hymexazole, azaconazole ), Dickine-M, oxopc Onazol), paclobutrazol, uniconazol, 1-(4-chloro-phenyl)-2-([1,2,4]triazol-1-yl)-cycloheptanol and sulfuric acid Imazalilsulfphate; eosin, including azoxystrobin, dimoxystrobin, enestroburin, fluoxastrobin, kresoxim- Methyl), methocycline (methominostrobin), orysastrobin, picoxystrobin, pyraclostrobin, trifloxystrobin, enestroburin, (2) -Chloro-5-[1-(3-methylbenzyloxyimino)ethyl]benzyl)carbamic acid methyl ester, (2-chloro-5-[1-(6-methylpyridine-2-) Methyl methoxyimino)ethyl]benzyl)carbamate and 2-(o-(2,5-dimethylphenoxymethylene)-phenyl)-3-methoxyacrylic acid Ester, 2-(2-(6-(3-chloro-2-methyl-phenoxy)-5-fluoro-pyrimidin-4-yloxy)-phenyl)-2-methoxyimino- N-methyl-acetamide and 3-methoxy-2-(2-(N-(4-methoxy-phenyl)-cyclopropane)-iminylthiomethyl)-phenyl) - methyl acrylate; carboxamide, including carboxin, benalaxyl Bendall-M, fenhexamid, flutolanil, furametpyr, mepronil, metalaxyl, mefenoxam, fur Ofurace, oxadixyl, oxycarboxin, penthiopyrad, isopyrazam, thifluzamide, tiadinil, 3,4-Dichloro-N-(2-cyanophenyl)isothiazol-5-carboxamide, dimethomorph, flumorph, flumetover, fluoropyridinium Fluopicolide (picobenzamid), zoxamide, carpropamid, diclocymet, mandipropamid, N-(2) -(4-[3-(4-chlorophenyl)prop-2-ynyloxy]-3-methoxyphenyl)ethyl)-2-methylsulfonyl-amino-3-methylbutane Amine, N-(2-(4-[3-(4-chloro-phenyl)prop-2-ynyloxy]-3-methoxy-phenyl)ethyl)-2-ethanesulfonylamino 3-methylbutyridamine, methyl 3-(4-chlorophenyl)-3-(2-isopropoxycarbonyl-amino-3-methyl-butylammonium)propionate, N-( 4'-bromobiphenyl-2-yl)-4-difluoromethyl^-methylthiazole-δ-formamide, N-(4'-three Methyl-biphenyl-2-yl)-4-difluoromethyl-2-methylthiazole-5-formamide, N-(4'-chloro-3'-fluorobiphenyl-2-yl)- 4-Difluoromethyl-2-methyl-thiazole-5-carboxamide, N-(3\4'-dichloro-4-fluorobiphenyl-2-yl)-3-difluoro-methyl- 1-methyl-pyrazole-4-carboxamide, N-(3',4'-dichloro-5-fluorobiphenyl-2-yl)-3-difluoromethyl-1-methylpyrazole 4-carbamamine, N-(2-cyano-phenyl)-3,4-dichloroisothiazol-5-carboxamide, 2-amino-4-methyl-thiazole-5-formamidine Aniline, 2-chloro-N-(1,1,3-trimethyl-indan-4-yl)-nicotinium amide, N-(2-(1,3-dimethylbutyl)-benzene -1,3-Dimethyl-5-fluoro-1H-pyrazole-4-carboxamide, N-(4'-chloro-3',5-difluoro-biphenyl-2-yl)- 3-Difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, N-(4'-chloro-3',5-difluoro-biphenyl-2-yl)-3-tri Fluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, N-(3',4'-dichloro-5-fluoro-biphenyl-2-yl)-3-trifluoromethyl 1-methyl-1H-pyrazole-4-carboxamide, N-(3',5-difluoro-4'-methyl-biphenyl-2-yl)-3-difluoromethyl-1 -Methyl-1H-pyrazole-4-carboxamide, N-(3',5-difluoro-4'-methyl-biphenyl-2-yl)-3-trifluoromethyl-1-methyl -1H-pyrazole-4-carboxamide, N-(cis-2-bicyclopropyl-2-yl-phenyl)-3-difluoro -1-methyl-1H-pyrazole-4-carbamide, N-(trans-2-bicyclopropyl-2-yl-phenyl)-3-difluoro-methyl-1-methyl- 1H-pyrazole-4-carboxamide, flupirtine, N-(3-ethyl-3,5-5-trimethyl-cyclohexyl)-3-carboxamido-2-hydroxy- Benzylamine, oxytetracycline, thioxanthene, N-(6-methoxy-pyridin-3-yl)cyclopropanecarbamide, 2-iodo-N-phenyl-benzamide, N- (2-bicyclo-propyl-2-yl-phenyl)-3-difluoromethyl-1-methylpyrazole-4-ylcarboxamide, N-(3',4',5'-three Fluorbiphenyl-2-yl)-1,3-dimethylpyrazole-4-ylcarboxamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-1, 3-Dimethyl-5-fluoropyrazole-4-yl-carbenamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-5-chloro-1,3- Dimethyl-pyrazol-4-ylcarboxamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-3-fluoromethyl-1-methylpyrazole-4 -carbamamine, N-(3',4',5'-trifluorobiphenyl-2-yl)-3-(chlorofluoromethyl)-1-methylpyrazol-4-ylcarboxamide , N-(3',4',5'-trifluorobiphenyl-2-yl)-3-difluoromethyl-1-methylpyrazol-4-ylcarboxamide, N-(3', 4',5'-Trifluorobiphenyl-2-yl)-3-difluoromethyl-5-fluoro-1-methylpyrazole-4-ylcarboxamide, N-(3',4', 5'-trifluorobiphenyl-2-yl)-5-chloro-3-difluoromethyl-1- Methylpyrazole-4-ylformamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-3-(chlorodifluoromethyl)-1-methylpyrazole 4-ylformamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-1-methyl-3-trifluoromethylpyrazole-4-ylcarboxamide , N-(3',4',5'-trifluorobiphenyl-2-yl)-5-fluoro-1-methyl-3-trifluoromethylpyrazole-4-ylcarboxamide, N- (3',4',5'-Trifluorobiphenyl-2-yl)-5-chloro-1-methyl-3-trifluoromethylpyrazole-4-ylcarboxamide, N-(2' , 4',5'-trifluorobiphenyl-2-yl)-1,3-dimethylpyrazole-4-ylcarboxamide, N-(2',4',5'-trifluorobiphenyl -2-yl)-1,3-dimethyl-5-fluoropyrazole-4-ylcarboxamide, N-(2',4',5'-trifluorobiphenyl-2-yl)-5 -Chloro-1,3-dimethylpyrazole-4-ylcarboxamide, N-(2',4',5'-trifluorobiphenyl-2-yl)-3-fluoromethyl-1- Methylpyrazol-4-ylcarboxamide, N-(2',4',5'-trifluorobiphenyl-2-yl)-3-(chlorofluoromethyl)-1-methylpyrazole- 4-ylformamide, N-(2',4',5'-trifluorobiphenyl-2-yl)-3-difluoromethyl-1-methylpyrazol-4-ylcarboxamide, N-(2',4',5'-trifluorobiphenyl-2-yl)-3-difluoromethyl-5-fluoro-1-methylpyrazole-4-ylcarboxamide, N-( 2',4',5'-Trifluorobiphenyl-2-yl)-5-chloro-3-difluoromethyl-1-methylpyrazole-4-ylcarboxamide, N-(2', 4', 5' -Trifluorobiphenyl-2-yl)-3-(chlorodifluoromethyl)-1-methylpyrazol-4-ylcarboxamide, N-(2',4',5'-trifluoro Phen-2-yl)-1-methyl-3-trifluoromethylpyrazole-4-ylcarboxamide, N-(2',4',5'-trifluorobiphenyl-2-yl)- 5-fluoro-1-methyl-3-trifluoromethylpyrazole-4-ylcarboxamide, N-(2',4',5'-trifluorobiphenyl-2-yl)-5-chloro -1-methyl-3-trifluoromethylpyrazole-4-ylcarboxamide, N-(3',4'-dichloro-3-fluorobiphenyl-2-yl)-1-methyl- 3-trifluoromethyl-1H-pyrazole-4-carboxamide, N-(3',4'-dichloro-3-fluorobiphenyl-2-yl)-1-methyl-3-difluoro Methyl-1H-pyrazole-4-carboxamide, N-(3',4'-difluoro-3-fluorobiphenyl-2-yl)-1-methyl-3-trifluoromethyl-1H -pyrazole-4-carbamide, N-(3',4'-difluoro-3-fluorobiphenyl-2-yl)-1-methyl-S-difluoromethyl-1H-pyrazole- 4-Protonamine, N-(3'-chloro-4'-fluoro-3-fluorobiphenyl-2-yl)-1-methyl-3-difluoromethyl-1H-pyrazole-4-A Indoleamine, N-(3',4'-dichloro-4-fluorobiphenyl-2-yl)-1-methyl-3-trifluoromethyl-1H-pyrazole-4-carboxamide, N -(3',4'-difluoro-4-fluorobiphenyl-2-yl)-1-methyl-S-trifluoromethyl-1H-pyrazole-4-carboxamide, N-(3' , 4'-Dichloro-4-fluorobiphenyl-2-yl)-1-methyl-3-difluoromethyl-1H-pyrazole-4-carboxamide, N-(3',4'- two 4-fluorobiphenyl-2-yl)-1-methyl-3-difluoromethyl-1H-pyrazole-4-carboxamide, N-(3'-chloro-4'-fluoro-4- Fluorbiphenyl-2-yl)-1-methyl-S-difluoromethyl-1H-pyrazole-4-carboxamide, N-(3',4'-dichloro-5-fluorobiphenyl- 2-yl)-1-methyl-3-trifluoromethyl-1H-pyrazole-4-carboxamide, N-(3',4'-difluoro-5-fluorobiphenyl-2-yl) -1-methyl-3-trifluoromethyl-1H-pyrazole-4-carboxamide, N-(3',4'-dichloro-5-fluorobiphenyl-2-yl)-1-methyl s-S-difluoromethyl-1H-pyrazole-formamide, N-(3',4'-difluoro-5-fluorobiphenyl-2-yl)-1-methyl-3-difluoro Methyl-1H-pyrazole-4-carboxamide, N-(3',4'-dichloro-5-fluorobiphenyl-2-yl)-1,3-dimethyl-1H-pyrazole- 4-Protonamine, N-(3'-chloro-4'-fluoro-5-fluorobiphenyl-2-yl)-1-methyl-3-difluoromethyl-1H-pyrazole-4-A Indoleamine, N-(4'-fluoro-4-fluorobiphenyl-2-yl)-1-methyl-3-trifluoromethyl-1H-pyrazole-4-carboxamide, N-(4' -Fluoro-5-fluorobiphenyl-2-yl)-1-methyl-3-trifluoromethyl-1H-pyrazole-4-carboxamide, N-(4'-chloro-5-fluorobiphenyl -2-yl)-1-methyl-3-trifluoromethyl-1H-pyrazole-4-carboxamide, N-(4'-methyl-5-fluorobiphenyl-2-yl)-1 -methyl-3-trifluoromethyl-1H-pyrazole-4-carboxamide, N-(4'-fluoro-5-fluorobiphenyl-2-yl)-1,3-dimethyl-1H - Oxazole-4-carbamide, N-(4'-chloro-5-fluorobiphenyl-2-yl)-1,3-dimethyl-1H-pyrazole-4-carboxamide, N-(4 '-Methyl-5-fluorobiphenyl-2-yl)-1,3-dimethyl-1H-pyrazole-4-carboxamide, N-(4'-fluoro-6-fluorobiphenyl-2 -yl)-1-methyl-3-trifluoromethyl-1H-pyrazole-4-carboxamide, N-(4'-chloro-6-fluorobiphenyl-2-yl)-1-methyl -3-trifluoromethyl-1H-pyrazole-4-carboxamide, N-[2-(1,1,2,3,3,3-hexafluoropropoxy)-phenyl]-3- Difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, N-[4'-(trifluoromethylthio)-biphenyl-2-yl]-3-difluoromethyl- 1-methyl-1H-pyrazole-4-carboxamide and N-[4'-(trifluoromethylthio)-biphenyl-2-yl]-1-methyl-3-trifluoromethyl- 1-methyl-1H-pyrazole-4-carboxamide; heterocyclic compounds, including fluazinam, pyrifenox, bupirimate, cyprodinil, Fenarimol, ferimzone, mepanipyrim, nuarimol, pyrimethanil, triforine, fenpiclonil, care Fludioxonil, aldimorph, dodemorph, fenpropimorph, tridemorph, benzene Fenpropidin, iprodione, procymidone, vinclozolin, famoxadone, fenamidone, octhilinone, proben -azole), 5-chloro-7-(4-methyl-piperidin-1-yl)-6-(2,4,6-trifluorophenyl)-[1,2,4]triazolo[ 1,5-a]pyrimidine, anilazine, diclomezine, pyroquilon, proquinazid, tricyclazole, 2-butoxy- 6-iodo-3-propyl Ace-4-one, acidified benzothiadiazole-S-methyl ester, captafol, captan, dazome, folfet, fenoxanil ), quinoxyfen, N,N-dimethyl-3-(3-bromo-6-fluoro-2-methylindole-1-sulfonyl)-[1,2,4]triazole 1-sulfonamide, 5-ethyl-6-octyl-[1,2,4]triazolo[1,5-a]pyrimidine-2,7-diamine, 2,3,5,6 -tetrachloro-4-methanesulfonyl-pyridine, 3,4,5-trichloro-pyridine-2,6-dicarbonitrile, N-(1-(5-bromo-3-chloro-pyridine-2- -ethyl)-2,4-dichloro-nicotinamide, N-((5-bromo-3-chloropyridin-2-yl)-methyl)-2,4-dichloro-nicotine Indoleamine, diflumetorim, nitrapyrin, dodemorphacetate, fluoroimid, blasticidin-S, sputum Chinomethionat), debacarb, difenzoquat, difenzoquat-methylsulphat, oxolinic acid, and piperalin; Acid esters, including manganzeb, maneb, manam, methasulphocarb, metiram, fere Bam), propineb, thiram, zineb, ziram, diethofencarb, iprovalicarb, phenothiamine Benthiavalicarb), propamocarb, downy mild acid hydrochloride, N-(1-(1-(4-cyanophenyl)-ethanesulfonyl)butan-2-yl)carbamic acid 4- Fluorophenyl ester, methyl 3-(4-chloro-phenyl)-3-(2-isopropoxycarbonylamino-3-methyl-butylguanidino)propionate; and other fungicides, including hydrazine , dodine, toxin free base, iminoctadine, guazatine; antibiotics: kasugamycin, oxytetracycline and its salts, streptomycin, granules Polyoxin, validamycin A; nitrophenyl derivatives: binapacryl, dinocap, dinobuton; sulfur-containing heterocyclic compounds: Dithianon, isoprothiolane; organometallic compounds: dip and tin; organic phosphorus compounds: edifenphos, iprobenfos, fosetyl, blessing Saide-aluminum, phosphorous acid and its salt, white pine (pyrazo Phos), tolclofos-methyl; organochlorine compounds: dichlofluanid, flusulfamide, hexachlorobenzene, phthalide, pencycuron, pentachloronitrate Quintozene, thiophanate, methylpreservative, tolylfluanid; others: cyflufenamid, cymoxanil, dimethirimol , ethirimol, furalaxyl, metrafenone and spiroxamine, biguanide acetate, iminoc-tadine-triacetate, gram heat Isooctadine-tris (albesilate), kasugamycin hydrochloride hydrate, dichlorophen, pentachlorophenol and its salts, N-(4-chloro -2-nitro-phenyl)-N-ethyl-4-methyl-benzenesulfonamide, chlorolamine (dicloran), nitrothal-isopropyl, tetrachloronitrobenzene ( Tectazen), biphenyl, bronopol, diphenylamine, mildiomycin, oxincopper, prohexadione calcium, N-( Propylmethoxyimino-(6-difluoromethoxy-2,3-difluoro-phenyl)-methyl)-2-phenylacetamide, N'-(4-(4- Chloro-3-trifluoromethyl-phenoxy)-2,5-dimethyl-phenyl)-N-ethyl-N-methylformamidine, N'-(4-(4-fluoro-3) -trifluoromethyl-phenoxy)-2,5-dimethyl-phenyl)-N-ethyl-N-methylformamidine, N'-(2-methyl-5-trifluoromethyl 4-(3-trimethyldecyl-propoxy)-phenyl)-N-ethyl-N-methylformamidine and N'-(5-difluoromethyl-2-methyl-4 -(3-Trimethyldecyl-propoxy)-phenyl)-N-ethyl-N-methylformamidine. 如請求項67之組合物，其中該殺真菌劑係選自由氟吡菌醯胺加上得克利、四氯異苯腈、甲基托布津、丙硫菌唑及氫氧化銅組成之群。 The composition of claim 67, wherein the fungicide is selected from the group consisting of fluopyram and dextran, tetrachloroisophthalonitrile, methyl thiophanate, prothioconazole, and copper hydroxide. 如請求項67之組合物，其中該組合物呈液體、粉劑、可撒佈顆 粒或乾燥可濕潤顆粒形式。 The composition of claim 67, wherein the composition is in the form of a liquid, a powder, or a spreadable Granular or dry in the form of wettable granules. 一種以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472之生物純培養物的提取物，該提取物包括以下中之至少一者：分離之豐原素MA化合物、分離之豐原素MB化合物、分離之豐原素MC化合物、分離之去羥基豐原素MA化合物、分離之去羥基豐原素MB化合物、分離之豐原素H化合物、分離之豐原素I化合物及分離之去羥基豐原素I化合物。 An extract of a biologically pure culture of Bacillus amyloliquefaciens RTI472 deposited under ATCC PTA-121166, the extract comprising at least one of the following: isolated abundance MA compound, isolated abundance MB compound, separation A protosulin MC compound, an isolated dehydroxyl-prolamin MA compound, an isolated dehydroxy-hydroxytoxin MB compound, an isolated abundance H compound, an isolated abundance I compound, and an isolated dehydroxy-hydroxytoxin I compound. 一種解澱粉芽孢桿菌之生物純培養物之提取物，該提取物包括以下至少一者：分子量等於3341.6Da之分離之Ericin S化合物，及分子量等於2985.4Da之分離之Ericin A化合物。 An extract of a biologically pure culture of B. amyloliquefaciens, the extract comprising at least one of the following: an isolated Ericin S compound having a molecular weight equal to 3341.6 Da, and an isolated Ericin A compound having a molecular weight equal to 2985.4 Da. 一種保護植物或果實免於病原性感染或治療植物或果實之病原性感染的方法，其包含將有效量之如請求項63至73中任一項之組合物或提取物施用於該植物或果實，或該等植物之根部或根部周圍土壤。 A method of protecting a plant or fruit from a pathogenic infection or treating a pathogenic infection of a plant or fruit, comprising applying an effective amount of the composition or extract of any one of claims 63 to 73 to the plant or fruit , or the soil around the roots or roots of such plants. 如請求項74之方法，其中該植物包含單子葉植物、雙子葉植物、穀物、玉米、甜玉米、爆玉米、玉米種、青貯玉米、飼料玉米、稻穀、小麥、大麥、高粱、蘆筍、漿果、藍莓、黑莓、樹莓、羅甘莓、越橘、蔓越橘、醋栗、接骨木、黑醋栗、蔓越莓、越莓、甘藍型蔬菜、椰菜、甘藍菜、花椰菜、芽甘藍、散葉甘藍、羽衣甘藍、芥菜、球莖甘藍、瓜類蔬菜、黃瓜、哈密瓜、甜瓜、香甘瓜、番南瓜、西瓜、南瓜、茄子、鱗莖類蔬菜、洋蔥、大蒜、蔥、陳皮、橘子、葡萄柚、檸檬、紅橘、橘柚、柚子、果蔬菜、胡椒、番茄、地面櫻桃、樹蕃茄、秋葵、葡萄、香草/香料、綠葉蔬菜、萵苣、芹菜、菠菜、香芹、菊苣、豆科植物/蔬菜(多汁且乾燥之豆子及豌豆)、豆子、四季豆、食莢菜豆、殼豆、大豆、乾豆、回回豆、利馬豆、豌豆、 鷹嘴豆、豌豆瓣、扁豆、油料種子作物、芥花、蓖麻、椰子、棉花、亞麻、油棕櫚、橄欖、花生、油菜籽、紅花、芝麻、向日葵、大豆、梨果、蘋果、海棠果、梨、榅桲、夏花山楂、根/塊莖及球莖蔬菜、胡蘿蔔、馬鈴薯、甘薯、木薯、甜菜、薑、辣根、蘿蔔、人參、蕪菁、核果、杏子、櫻桃、油桃、桃子、李子、梅子、草莓、堅果、杏仁、開心果、山核桃、核桃、榛子、板栗、腰果、山毛櫸堅果、胡桃、夏威夷果、獼猴桃、香蕉、(藍)龍舌蘭、草、草皮草、觀賞植物、一品紅、硬木剪枝、栗樹、橡樹、楓樹、甘蔗或甜菜。 The method of claim 74, wherein the plant comprises monocots, dicots, grains, corn, sweet corn, popcorn, corn, silage corn, forage corn, rice, wheat, barley, sorghum, asparagus, berries, Blueberries, blackberries, raspberries, raspberries, cranberries, cranberries, gooseberries, elderberries, currants, cranberries, berry, cabbage, broccoli, kale, broccoli, Brussels sprouts, Cabbage, kale, mustard, stalk, melon, cucumber, cantaloupe, melon, sweet melon, squash, watermelon, pumpkin, eggplant, bulbous vegetables, onions, garlic, onions, tangerine peel, oranges, grapes Pomelo, lemon, red orange, tangerine, grapefruit, fruit vegetable, pepper, tomato, ground cherry, tree tomato, okra, grape, vanilla/spice, green leafy vegetables, lettuce, celery, spinach, parsley, chicory, legume Plants/vegetables (succulent and dry beans and peas), beans, green beans, food pods, shell beans, soybeans, dried beans, peas, lima beans, peas, Chickpeas, pea petals, lentils, oilseed crops, canola, ramie, coconut, cotton, flax, oil palm, olive, peanut, rapeseed, safflower, sesame, sunflower, soybean, pear, apple, jellyfish, Pear, medlar, summer flower hawthorn, root/tuber and bulbous vegetables, carrots, potatoes, sweet potatoes, cassava, beets, ginger, horseradish, radish, ginseng, turnip, stone fruit, apricot, cherry, nectarine, peach, plum, Plum, strawberry, nut, almond, pistachio, pecan, walnut, hazelnut, chestnut, cashew, beech nut, walnut, macadamia, kiwi, banana, (blue) agave, grass, turf, ornamental, poinsettia , hardwood pruning, chestnut, oak, maple, sugar cane or beet. 如請求項74之方法，其中該病原性感染由以下引起：植物真菌病原體、植物細菌病原體、鏽菌、葡萄孢屬、灰葡萄孢菌、蔥鱗葡萄孢菌、歐文菌屬、胡蘿蔔軟腐歐文菌、解澱粉歐文菌、狄克氏菌屬、達旦狄克氏菌、黑脛病菌、土壤桿菌屬、根癌土壤桿菌、黃單孢菌屬、地毯草黃單孢菌、油菜黃單孢菌胡蘿蔔致病變種、桃李黃單孢菌、核桃黃單孢菌、水稻黃單孢菌水稻變種、焦枯菌屬、葉緣焦枯菌、卡地菌屬、韌皮部桿菌、鐮刀菌屬、大刀鐮刀菌、禾穀鐮刀菌、尖孢鐮刀菌、古巴尖孢鐮刀菌、番茄尖孢鐮刀菌、棒形鐮刀菌、核盤菌屬、向日葵核盤菌、小核盤菌、同果核盤菌、尾孢菌屬、鉤絲殼屬、葡萄白粉病菌(白粉病)、叉絲單囊殼屬(白粉病)、白叉絲單囊殼、克蘭德叉絲單囊殼、擬莖點菌屬、葡萄擬莖點黴病菌、交鏈孢菌屬、細極鏈格孢菌、蔥鏈格孢菌、交鏈格孢菌、立枯交鏈孢菌、鏈格孢菌、假單胞菌屬、丁香假單胞菌番茄變種、疫黴菌屬、致病疫黴菌、寄生疫黴菌、大豆疫黴菌、辣椒疫黴菌、樟疫黴菌、草莓疫黴菌、疫黴菌屬、櫟樹猝死病菌、棕櫚疫黴菌、菸草疫黴菌、層鏽菌屬、豆薯層鏽菌、層鏽層假尾孢菌、曲黴菌 屬、黃麴黴、黑麯黴、單孢鏽菌屬、菜豆銹病菌、枝孢菌屬、多主枝孢黴、根黴菌屬、少根根黴菌、青黴菌屬、絲核菌屬、立枯絲核菌、玉蜀黍絲核菌、稻枯斑絲核菌、褐色絲核菌、禾穀絲核菌、紫紋羽絲核菌、草莓絲核菌、枝生絲核菌、覆盆子絲核菌、豆狀絲核菌、菜豆殼球孢菌、稻瘟菌、球腔菌屬、禾生球腔菌、斐濟球腔菌(黑色香蕉葉斑病)、蘋果斑點球腔菌、檸檬球腔菌、稻瘟菌屬、稻瘟病菌、鏈核盤菌屬、桃褐腐病菌、藍莓僵果病病原菌、核果鏈核盤菌、炭疽菌屬、膠孢炭疽菌、辣椒炭疽菌、石斛炭疽菌、間座殼屬、柑桔間座殼菌、棒孢菌屬、多主棒孢菌、膠鏽菌屬、植物受檜膠鏽菌、裂盾菌屬、仁果裂盾菌、黏殼孢菌屬、梨果黏殼孢菌、葡萄座腔菌屬、葡萄潰瘍病病菌、明孢盤菌屬、威爾遜孢殼菌屬、嗜果威爾遜孢殼菌、單囊殼菌屬、斑點單囊殼菌、薔薇單囊殼菌、白粉菌屬、殼多孢菌屬、穎枯殼針孢、腐黴屬、終極腐黴菌、瓜果腐黴菌、畸雌腐黴菌、簇囊腐黴菌、水生腐黴菌、森林腐黴菌、黑星菌屬、蘋果黑星病菌、輪枝孢菌屬、黑粉菌屬、裸黑粉菌、玉蜀黍黑粉菌、甘蔗鞭黑粉菌、麥角菌屬、麥角菌、腥黑粉菌屬、小麥腥黑粉菌、光滑腥黑粉菌、稻粒黑粉菌、小麥矮腥黑粉菌、莖點黴屬、大豆生莖點黴、多變莖點黴、甘藍莖點黴、禾旋孢腔菌、禾頂囊殼、炭疽菌屬、雲紋病菌屬、大麥雲紋病菌、離蠕孢屬、長蠕孢屬、大麥長蠕孢、玉蜀黍長蠕孢、索萊長蠕孢、小麥褐斑長蠕孢或其組合。 The method of claim 74, wherein the pathogenic infection is caused by a plant fungal pathogen, a plant bacterial pathogen, a rust fungus, a Botrytis, a Botrytis cinerea, a Botrytis cinerea, an Erwinia, and a carrot soft rot Starch Amoeba, Diracium, D. dandelion, black smut, Agrobacterium, Agrobacterium tumefaciens, Xanthomonas, P. sphaeroides, Xanthomonas campestris Pathogenic species, Rhizoctonia solani, Rhizoctonia solani, Rice variety of Xanthomonas oryzae, Saccharomyces, Saccharomyces cerevisiae, Cartilian, Phloem phlei, Fusarium, Fusarium oxysporum, Wo Fusarium oxysporum, Fusarium oxysporum, Fusarium oxysporum, Fusarium oxysporum, Fusarium solani, Sclerotinia, S. sclerotiorum, Sclerotinia sclerotiorum, Sclerotinia sclerotiorum, Cercospora Genus, genus, genus, powdery mildew (white powder), genus of the genus of the genus of the genus (white powder), white stalk, single sac, clad, single sac, stalk, stalk Phomopsis solani, Alternaria, Alternaria alternata, onion chain Alternaria, Alternaria alternata, Alternaria alternata, Alternaria, Pseudomonas, Pseudomonas syringae, tomato Phytophthora, Phytophthora infestans, Phytophthora infestans, Phytophthora sojae Phytophthora capsici, Phytophthora capsici, Phytophthora infestans, Phytophthora, eucalyptus, Phytophthora palmatum, Phytophthora nicotianae, rust fungus, rust fungus, rust layer pseudomonas, koji Mold Genus, Astragalus, Aspergillus niger, Puccinia rot, Bean rust, Cladosporium, Cladosporium, Rhizopus, Rhizopus, Penicillium, Rhizoctonia, Lichen Rhizoctonia, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia cerealis, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia granulosus, Rhizoctonia solani, Phytophthora sojae, Magnaporthe oryzae, Phytophthora, Phytophthora, Ficus sclerotium (black banana leaf spot), Campouflagellate, Lemon bacterium, rice Trichophyton, Magnaporthe oryzae, Sclerotinia sclerotiorum, Peach brown rot fungus, Blueberry fruit disease pathogen, Spirulina platensis, Anthrax, Anthrax anthracis, Capsicum anthracis, Anthracnose, Acacia Genus, citrus, genus, genus, genus, genus, genus, genus, genus, genus, genus, genus, genus, genus, genus Pythium oxysporum, genus genus, grape canker, genus, genus, genus, genus, genus, genus, genus Point sclerotium, Rhizoctonia solani, Powdery mildew, genus Polyporus, Needle, Pythium, Pythium, Pythium, Pythium, Pythium , Pythium oxysporum, Pythium, Pseudomonas, Escherichia coli, Verticillium, Black powder, Naked black powder, Black powder, Sugarcane, Saccharomyces , ergot, smut, wheat smut, sphaerotheca sphaeroides, rice black powder fungus, wheat dwarf smut, stalk genus, soybean stalk, variegated stalk Spot mold, Brassica oleracea, Trichomonas serrata, Hessian shell, Anthrax, Rhizoctonia, Barley, Rhizoctonia, Helminthosporium, Long Helminthum, Long worm, Yulong Helminthosporium, Solanum velutipes, Wheat Helminthosporium or a combination thereof. 一種藉由對易感病植物賦予保護以避免病原性感染或減少病原性感染使植物生長獲益，同時使針對處理之抗性積聚減至最少之方法，該方法包含：將第一組合物及第二組合物以分開施用及改變時間間隔輸送 至該易感病植物，其中該第一組合物及該第二組合物各者以適合於對該植物賦予保護以避免病原性感染或減少病原性感染之量輸送，其中該第一組合物包含以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物；且其中該第二組合物包含一或多種具有殺真菌或殺菌特性之化學活性劑，且其中該第一組合物及該第二組合物以該等改變時間間隔輸送至該植物之葉、該植物之莖皮、該植物之果實、該植物之花、該植物之種子、該植物之根部、該植物之剪枝、該植物之移植物、該植物之癒合組織或該植物周圍土壤或生長介質之一或組合，其中賦予保護以避免該病原性感染及/或減少該病原性感染所需之該(等)化學活性劑的總量減少且針對處理之抗性積聚減至最少。 A method for providing plant protection by protecting a susceptible plant from pathogenic infection or reducing pathogenic infection while minimizing the accumulation of resistance to treatment, the method comprising: The second composition is delivered separately and at varying intervals To the susceptible plant, wherein the first composition and the second composition are each delivered in an amount suitable to confer protection to the plant to avoid pathogenic infection or reduce pathogenic infection, wherein the first composition comprises A biologically pure culture of Bacillus amyloliquefaciens RTI472 or a mutant having all of its identifying characteristics deposited with ATCC PTA-121166; and wherein the second composition comprises one or more chemical activities having fungicidal or bactericidal properties And wherein the first composition and the second composition are delivered to the leaves of the plant, the bark of the plant, the fruit of the plant, the flower of the plant, the seed of the plant, at the time interval of the change, One or a combination of the root of the plant, the pruning of the plant, the graft of the plant, the healing tissue of the plant, or the soil or growth medium surrounding the plant, wherein protection is provided to avoid the pathogenic infection and/or reduce the pathogenicity The total amount of the (etc.) chemically active agent required for infection is reduced and the accumulation of resistance to treatment is minimized. 如請求項77之方法，其中該植物包含單子葉植物、雙子葉植物、穀物、玉米、甜玉米、爆玉米、玉米種、青貯玉米、飼料玉米、稻穀、小麥、大麥、高粱、蘆筍、漿果、藍莓、黑莓、樹莓、羅甘莓、越橘、蔓越橘、醋栗、接骨木、黑醋栗、蔓越莓、越莓、甘藍型蔬菜、椰菜、甘藍菜、花椰菜、芽甘藍、散葉甘藍、羽衣甘藍、芥菜、球莖甘藍、南瓜、茄子、瓜類蔬菜、黃瓜、哈密瓜、甜瓜、香甘瓜、番南瓜、西瓜、鱗莖類蔬菜、洋蔥、大蒜、蔥、陳皮、橘子、葡萄柚、檸檬、紅橘、橘柚、柚子、果蔬菜、胡椒、番茄、地面櫻桃、樹蕃茄、秋葵、葡萄、香草/香料、綠葉蔬菜、萵苣、芹菜、菠菜、香芹、菊 苣、豆科植物/蔬菜(多汁且乾燥之豆子及豌豆)、豆子、四季豆、食莢菜豆、殼豆、大豆、乾豆、回回豆、利馬豆、豌豆、鷹嘴豆、豌豆瓣、扁豆、油料種子作物、芥花、蓖麻、椰子、棉花、亞麻、油棕櫚、橄欖、花生、油菜籽、紅花、芝麻、向日葵、大豆、梨果、蘋果、海棠果、梨、榅桲、夏花山楂、根/塊莖及球莖蔬菜、胡蘿蔔、馬鈴薯、甘薯、木薯、甜菜、薑、辣根、蘿蔔、人參、蕪菁、核果、杏子、櫻桃、油桃、桃子、李子、梅子、草莓、堅果、杏仁、開心果、山核桃、核桃、榛子、板栗、腰果、山毛櫸堅果、胡桃、夏威夷果、獼猴桃、香蕉、(藍)龍舌蘭、草、草皮草、觀賞植物、一品紅、硬木剪枝、栗樹、橡樹、楓樹、甘蔗或甜菜。 The method of claim 77, wherein the plant comprises monocots, dicots, grains, corn, sweet corn, popcorn, corn, silage corn, feed corn, rice, wheat, barley, sorghum, asparagus, berries, Blueberries, blackberries, raspberries, raspberries, cranberries, cranberries, gooseberries, elderberries, currants, cranberries, berry, cabbage, broccoli, kale, broccoli, Brussels sprouts, Amaranth, kale, mustard, stalk, pumpkin, eggplant, melon, cucumber, cantaloupe, melon, sweet melon, squash, watermelon, bulbous vegetables, onions, garlic, onions, tangerine peel, oranges, grapes Pomelo, lemon, red orange, tangerine, grapefruit, fruit vegetable, pepper, tomato, ground cherry, tree tomato, okra, grape, vanilla/spice, green leafy vegetables, lettuce, celery, spinach, parsley, chrysanthemum Borage, legumes/vegetables (succulent and dry beans and peas), beans, green beans, pod beans, shell beans, soybeans, dried beans, peas, lima beans, peas, chickpeas, pea petals, Lentils, oilseed crops, canola, ramie, coconut, cotton, flax, oil palm, olive, peanut, rapeseed, safflower, sesame, sunflower, soybean, pear, apple, jellyfish, pear, alfalfa, summer Flowering hawthorn, roots/tubers and bulbous vegetables, carrots, potatoes, sweet potatoes, cassava, beets, ginger, horseradish, radish, ginseng, turnip, stone fruit, apricots, cherries, nectarines, peaches, plums, plums, strawberries, nuts, Almond, pistachio, pecan, walnut, hazelnut, chestnut, cashew, beech nut, walnut, macadamia, kiwi, banana, (blue) agave, grass, turf, ornamental, poinsettia, hardwood pruning, chestnut Trees, oaks, maples, sugar cane or beets. 如請求項77之方法，其中該病原性感染由以下引起：植物真菌病原體、植物細菌病原體、鏽菌、葡萄孢屬、灰葡萄孢菌、蔥鱗葡萄孢菌、歐文菌屬、胡蘿蔔軟腐歐文菌、解澱粉歐文菌、狄克氏菌屬、達旦狄克氏菌、黑脛病菌、土壤桿菌屬、根癌土壤桿菌、黃單孢菌屬、地毯草黃單孢菌、油菜黃單孢菌胡蘿蔔致病變種、桃李黃單孢菌、核桃黃單孢菌、水稻黃單孢菌水稻變種、焦枯菌屬、葉緣焦枯菌、卡地菌屬、韌皮部桿菌、鐮刀菌屬、大刀鐮刀菌、禾穀鐮刀菌、尖孢鐮刀菌、古巴尖孢鐮刀菌、番茄尖孢鐮刀菌、棒形鐮刀菌、核盤菌屬、向日葵核盤菌、小核盤菌、同果核盤菌、尾孢菌屬、鉤絲殼屬、葡萄白粉病菌(白粉病)、叉絲單囊殼屬(白粉病)、白叉絲單囊殼、克蘭德叉絲單囊殼、擬莖點菌屬、葡萄擬莖點黴病菌、交鏈孢菌屬、細極鏈格孢菌、蔥鏈格孢菌、交鏈格孢菌、立枯交鏈孢菌、鏈格孢菌、假單胞菌屬、丁香假單胞菌番茄變種、疫黴菌屬、致病疫黴菌、寄生疫黴菌、大豆疫黴菌、辣椒疫黴菌、樟疫黴 菌、草莓疫黴菌、疫黴菌屬、櫟樹猝死病菌、棕櫚疫黴菌、菸草疫黴菌、層鏽菌屬、豆薯層鏽菌、層鏽層假尾孢菌、曲黴菌屬、黃麴黴、黑麯黴、單孢鏽菌屬、菜豆銹病菌、枝孢菌屬、多主枝孢黴、根黴菌屬、少根根黴菌、青黴菌屬、絲核菌屬、立枯絲核菌、玉蜀黍絲核菌、稻枯斑絲核菌、褐色絲核菌、禾穀絲核菌、紫紋羽絲核菌、草莓絲核菌、枝生絲核菌、覆盆子絲核菌、豆狀絲核菌、菜豆殼球孢菌、稻瘟菌、球腔菌屬、禾生球腔菌、斐濟球腔菌(黑色香蕉葉斑病)、蘋果斑點球腔菌、檸檬球腔菌、稻瘟菌屬、稻瘟病菌、鏈核盤菌屬、桃褐腐病菌、藍莓僵果病病原菌、核果鏈核盤菌、炭疽菌屬、膠孢炭疽菌、辣椒炭疽菌、石斛炭疽菌、間座殼屬、柑桔間座殼菌、棒孢菌屬、多主棒孢菌、膠鏽菌屬、植物受檜膠鏽菌、裂盾菌屬、仁果裂盾菌、黏殼孢菌屬、梨果黏殼孢菌、葡萄座腔菌屬、葡萄潰瘍病病菌、明孢盤菌屬、威爾遜孢殼菌屬、嗜果威爾遜孢殼菌、單囊殼菌屬、斑點單囊殼菌、薔薇單囊殼菌、白粉菌屬、殼多孢菌屬、穎枯殼針孢、腐黴屬、終極腐黴菌、瓜果腐黴菌、畸雌腐黴菌、簇囊腐黴菌、水生腐黴菌、森林腐黴菌、黑星菌屬、蘋果黑星病菌、輪枝孢菌屬、黑粉菌屬、裸黑粉菌、玉蜀黍黑粉菌、甘蔗鞭黑粉菌、麥角菌屬、麥角菌、腥黑粉菌屬、小麥腥黑粉菌、光滑腥黑粉菌、稻粒黑粉菌、小麥矮腥黑粉菌、莖點黴屬、大豆生莖點黴、多變莖點黴、甘藍莖點黴、禾旋孢腔菌、禾頂囊殼、炭疽菌屬、雲紋病菌屬、大麥雲紋病菌、離蠕孢屬、長蠕孢屬、大麥長蠕孢、玉蜀黍長蠕孢、索萊長蠕孢、小麥褐斑長蠕孢或其組合。 The method of claim 77, wherein the pathogenic infection is caused by a plant fungal pathogen, a plant bacterial pathogen, a rust fungus, a Botrytis, a Botrytis cinerea, a Botrytis cinerea, an Erwinia, and a carrot soft rot Starch Amoeba, Diracium, D. dandelion, black smut, Agrobacterium, Agrobacterium tumefaciens, Xanthomonas, P. sphaeroides, Xanthomonas campestris Pathogenic species, Rhizoctonia solani, Rhizoctonia solani, Rice variety of Xanthomonas oryzae, Saccharomyces, Saccharomyces cerevisiae, Cartilian, Phloem phlei, Fusarium, Fusarium oxysporum, Wo Fusarium oxysporum, Fusarium oxysporum, Fusarium oxysporum, Fusarium oxysporum, Fusarium solani, Sclerotinia, S. sclerotiorum, Sclerotinia sclerotiorum, Sclerotinia sclerotiorum, Cercospora Genus, genus, genus, powdery mildew (white powder), genus of the genus of the genus of the genus (white powder), white stalk, single sac, clad, single sac, stalk, stalk Phomopsis solani, Alternaria, Alternaria alternata, onion chain Alternaria, Alternaria alternata, Alternaria alternata, Alternaria, Pseudomonas, Pseudomonas syringae, tomato Phytophthora, Phytophthora infestans, Phytophthora infestans, Phytophthora sojae Phytophthora capsici, Phytophthora capsici Phytophthora, Phytophthora infestans, Phytophthora, eucalyptus, Phytophthora palmatum, Phytophthora nicotianae, rust fungus, rust fungus, rust layer pseudomonas, Aspergillus, Astragalus, Aspergillus niger, Puccinia solani, Bean rust, Cladosporium, Cladosporium, Rhizopus, Rhizopus, Penicillium, Rhizoctonia, Rhizoctonia solani, Jade silk Nuclear bacteria, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia cerealis, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia solani, Rhizoctonia solani, Phytophthora, Magnaporthe oryzae, Phytophthora, Phytophthora, Fibroin bacterium (black banana leaf spot), Campouflagellate, Pyrococcus sphaeroides, Magnaporthe oryzae, Rice blast Bacteria, Sclerotinia, Peach brown rot, Blueberry zombie pathogen, Sclerotinia sclerotiorum, Anthrax, Anthrax anthracis, Capsicum anthracis, Anthracnose, Aphis, Citrus Crustacea, Corynebacterium, Pseudomonas aeruginosa, Puccinia, Plant Puccinia, Schizophyllum, R. sclerophylla, C. Genus, Phaeocystis, Phytophthora, Grape Canker, Campylobacter, Wilson, Phytophthora, Monochaete, Monocystis , Rhizoctonia solani, powdery mildew, genus Polyporus, Phytophthora, Pythium, Pythium, Pythium, Pythium, Pythium, Pythium, Forest Pythium, Black Spot, Apple Black Spot, Verticillium, Sphaerotheca, Naked Black Powder, Jade Black Powder, Sugarcane Grifola, Trichosanthes, Ergot,腥Black genus, wheat smut, sphaerotheca fuliginea, rice black powder fungus, wheat dwarf smut, stalk genus, soybean stalk, stalk, stalk Mold, Trichomonas, Trichoderma, Capsule, Anthrax, Rhizoctonia solani, Barley Phytophthora, Helminthosporium, Helminthosporium, Helminthosporium, Helminthosporium, Soleil Helminthosporium, Wheat Helminthosporium or a combination thereof. 如請求項77之方法，其中該等改變時間間隔為相隔5至7天。 The method of claim 77, wherein the change intervals are between 5 and 7 days apart. 如請求項77之方法，其中該第一組合物及該第二組合物各者輸 送至該植物之葉、該植物之果實或該植物之花。 The method of claim 77, wherein the first composition and the second composition are each lost The leaf that is sent to the plant, the fruit of the plant, or the flower of the plant. 如請求項77之方法，其中該適合於對該植物賦予保護以避免病原性感染或減少病原性感染之量為約1.0×1010CFU/ha至約1.0×1014CFU/ha之解澱粉芽孢桿菌RTI472。 The method of claim 77, wherein the starch-spore is suitable for imparting protection to the plant to avoid pathogenic infection or reducing pathogenic infection in an amount of from about 1.0 x 10 10 CFU/ha to about 1.0 x 10 14 CFU/ha. Bacillus RTI472. 如請求項77之方法，其中該化學活性劑包含以下之一或組合：甲氧基丙烯酸酯(strobilurine)、***、護汰芬(flutriafol)、得克利、丙硫菌唑(prothiaconazole)、氟環唑(expoxyconazole)、氟吡菌醯胺、四氯異苯腈、甲基托布津、氫氧化銅殺真菌劑、基於EDBC之殺真菌劑、鋅錳乃浦(mancozeb)、琥珀酸去氫酶(succinase dehydrogenase，SDHI)殺真菌劑、必殺吩(bixafen)、依普同(iprodione)、達滅芬(dimethomorph)或威利芬那雷特(valifenalate)。 The method of claim 77, wherein the chemically active agent comprises one or a combination of: strobilurine, triazole, flutriafol, dextran, prothiaconazole, fluoro Expoxazole (expoxyconazole), fluopyram, tetrachloroisophthalonitrile, methyl thiophanate, copper hydroxide fungicide, EDBC-based fungicide, zinc-manganese (mancozeb), succinate dehydrogenase (succinase dehydrogenase, SDHI) fungicide, bixafen, iprodione, dimethomorph or valifenalate. 如請求項83之方法，其中該化學活性劑包含氟吡菌醯胺加上得克利，且包含該RTI472之該第一組合物之輸送代替該四氯異苯腈殺真菌劑之輸送。 The method of claim 83, wherein the chemically active agent comprises flupirtine plus dextran, and the delivery of the first composition comprising the RTI 472 replaces the delivery of the tetrachloroisophthalonitrile fungicide. 如請求項83之方法，其中該植物包含葫蘆，且該病原性感染係由白粉病引起。 The method of claim 83, wherein the plant comprises a gourd and the pathogenic infection is caused by powdery mildew. 如請求項83之方法，其中該化學活性劑包含甲基托布津殺真菌劑，且包含該RTI472之該第一組合物之輸送代替丙硫菌唑殺真菌劑之輸送。 The method of claim 83, wherein the chemically active agent comprises a methyl thiophanate fungicide, and the delivery of the first composition comprising the RTI 472 is substituted for the delivery of the prothioconazole fungicide. 如請求項83之方法，其中該化學活性劑包含氫氧化銅殺真菌劑，且包含該RTI472之該第一組合物之輸送代替該四氯異苯腈殺真菌劑之輸送。 The method of claim 83, wherein the chemically active agent comprises a copper hydroxide fungicide, and the delivery of the first composition comprising the RTI 472 replaces the delivery of the tetrachloroisophthalonitrile fungicide. 如請求項83之方法，其中該第一組合物進一步包含以下之一或組合：載劑、界面活性劑、分散劑或酵母提取物。 The method of claim 83, wherein the first composition further comprises one or a combination of: a carrier, a surfactant, a dispersant, or a yeast extract. 一種使植物生長獲益之組合物，該組合物包含： 以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物；及畢芬寧(bifenthrin)殺蟲劑。 A composition that benefits plant growth, the composition comprising: A biologically pure culture of Bacillus amyloliquefaciens RTI472 or a mutant having all of its identifying characteristics deposited with ATCC PTA-121166; and bifenthrin insecticide. 如請求項89之組合物，其中該組合物呈與液體肥料相容之調配物。 The composition of claim 89, wherein the composition is in a formulation compatible with a liquid fertilizer. 如請求項89之組合物，其中該組合物進一步包含水合鋁-鎂矽酸鹽及至少一種分散劑。 The composition of claim 89, wherein the composition further comprises hydrated aluminum-magnesium citrate and at least one dispersant. 如請求項89之組合物，其中該畢芬寧殺蟲劑係以在0.1g/ml至0.2g/ml範圍內之濃度存在。 The composition of claim 89, wherein the Phenfenin insecticide is present in a concentration ranging from 0.1 g/ml to 0.2 g/ml. 如請求項89之組合物，其中該畢芬寧殺蟲劑係以約0.1715g/ml之濃度存在。 The composition of claim 89, wherein the Phenfenin insecticide is present at a concentration of about 0.1715 g/ml. 一種用於使植物生長獲益之組合物，該組合物包含以下：以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物；及包含來自白羽扇豆之提取物、BLAD多肽或BLAD多肽之片段之一或組合的殺真菌劑。 A composition for benefiting plant growth, the composition comprising: a Bacillus amyloliquefaciens RTI472 deposited with ATCC No. PTA-121166 or a biologically pure culture thereof having mutants having all of its identifying characteristics; A fungicide derived from one or a combination of an extract of white lupin, a BLAD polypeptide or a BLAD polypeptide. 如請求項94之組合物，其中該組合物包含約20%該BLAD多肽或該BLAD多肽之片段。 The composition of claim 94, wherein the composition comprises about 20% of the BLAD polypeptide or a fragment of the BLAD polypeptide. 一種產品，其包含：第一組分，該第一組分包含以ATCC第PTA-121166號寄存之解澱粉芽孢桿菌RTI472或其具有其所有鑑別特徵之突變體的生物純培養物；第二組分，該第二組分包含微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑、除草劑、植物提取物、植物生長調節劑或肥料之一或組合，其中該第一組分及該第二組分分開包裝，且其中各組分為適合於使植物生長獲益及/或對易感病植 物賦予保護以避免病原性感染之量；以及說明書，該說明書係針對用於輸送適合於使植物生長獲益之量的該第一組合物與該第二組合物之組合至：該植物之葉、該植物之莖皮、該植物之果實、該植物之花、該植物之種子、該植物之根部、該植物之剪枝、該植物之移植物、該植物之癒合組織；該植物之周圍土壤或生長介質；在土壤或生長介質中播種該植物之種子之前的該土壤或生長介質；或在土壤或生長介質中種植該植物、該植物剪枝、該植物移植物或該植物癒合組織之前的該土壤或生長介質。 A product comprising: a first component comprising a Bacillus amyloliquefaciens RTI472 deposited with ATCC PTA-121166 or a biologically pure culture thereof having mutants having all of its identifying characteristics; The second component comprises one or a combination of a microorganism, a biological or chemical insecticide, a fungicide, a nematicide, a bactericide, a herbicide, a plant extract, a plant growth regulator or a fertilizer, wherein the first component The component and the second component are packaged separately, and wherein each component is suitable for plant growth benefit and/or for susceptible disease The substance confers protection against the amount of pathogenic infection; and the instructions are directed to the combination of the first composition and the second composition for delivering an amount suitable for plant growth benefit to: the leaf of the plant , the bark of the plant, the fruit of the plant, the flower of the plant, the seed of the plant, the root of the plant, the pruning of the plant, the graft of the plant, the healing tissue of the plant; the soil surrounding the plant Or a growth medium; the soil or growth medium prior to seeding the seed of the plant in soil or growth medium; or planting the plant, the plant pruning, the plant graft or the plant healing tissue prior to the soil or growth medium The soil or growth medium. 如請求項96之產品，其中該殺蟲劑為擬除蟲菊酯(pyrethroid)、畢芬寧、七氟菊酯(tefluthrin)、ξ-賽滅寧、有機磷酸酯、氯氧磷(chlorethoxyphos)、陶斯松(chlorpyrifos)、丁基嘧啶磷(tebupirimfos)、賽扶寧(cyfluthrin)、非潑羅(fiprole)、費普尼(fipronil)、菸鹼類(nicotinoid)或可尼丁(clothianidin)之一或組合。 The product of claim 96, wherein the insecticide is pyrethroid, bifennin, tefluthrin, sputum-seding, organic phosphate, chlorethoxyphos, taosson One or a combination of (chlorpyrifos), tebupirimfos, cyfluthrin, fiprole, fipronil, nicotinoid or clothianidin . 如請求項96之產品，其中該第一組合物進一步包含以下之一或組合：載劑、界面活性劑、分散劑或酵母提取物。 The product of claim 96, wherein the first composition further comprises one or a combination of: a carrier, a surfactant, a dispersant, or a yeast extract. 如請求項96之產品，其中該第一組合物及該第二組合物各者呈液體、粉劑、可撒佈顆粒、乾燥可潤濕粉末或乾燥可潤濕顆粒形式。 The product of claim 96, wherein the first composition and the second composition are each in the form of a liquid, a powder, a spreadable granule, a dry wettable powder or a dry wettable granule. 如請求項96之產品，其中該第一組合物呈液體形式，且該解澱粉芽孢桿菌RTI472係以約1.0×108CFU/ml至約1.0×1012CFU/ml之濃度存在。 The product of claim 96, wherein the first composition is in liquid form and the B. amyloliquefaciens RTI 472 is present at a concentration of from about 1.0 x 10 8 CFU/ml to about 1.0 x 10 12 CFU/ml. 如請求項96之產品，其中該第一組合物呈粉劑、乾燥可潤濕粉末、可撒佈顆粒或乾燥可潤濕顆粒形式，且該解澱粉芽孢桿菌RTI472以約1.0×108CFU/g至約1.0×1012CFU/g之量存在。 The product of claim 96, wherein the first composition is in the form of a powder, a dry wettable powder, a spreadable granule or a dry wettable granule, and the B. amyloliquefaciform RTI 472 is at about 1.0 x 10 8 CFU/g It is present in an amount of about 1.0 x 10 12 CFU/g. 如請求項96之產品，其中該第一組合物呈油性分散液形式且該解澱粉芽孢桿菌RTI472係以約1.0×108CFU/ml至約1.0×1012CFU/ml之濃度存在。 The product of claim 96, wherein the first composition is in the form of an oily dispersion and the B. amyloliquefaciens RTI 472 is present at a concentration of from about 1.0 x 10 8 CFU/ml to about 1.0 x 10 12 CFU/ml. 一種組合物，其用於施用於植物以達成使植物生長獲益或對易感病植物賦予保護以避免病原性感染中之一或兩者，該組合物包含：具有使植物生長或植物健康中之一或兩者受益的特性之一或多種微生物的生物純培養物；以及酵母提取物。 A composition for application to a plant to achieve or benefit from the growth of a susceptible plant to avoid one or both of the pathogenic infections, the composition comprising: having plant growth or plant health One or both of the properties that benefit from the biologically pure culture of one or more microorganisms; and the yeast extract. 如請求項103之組合物，其進一步包含以下之一或組合：載劑、界面活性劑或分散劑。 The composition of claim 103, further comprising one or a combination of: a carrier, a surfactant, or a dispersing agent. 如請求項103之組合物，其進一步包含以下之一或組合：微生物、生物或化學殺蟲劑、殺真菌劑、殺線蟲劑、殺菌劑、除草劑、植物提取物、植物生長調節劑或肥料。 The composition of claim 103, further comprising one or a combination of: a microbial, biological or chemical insecticide, fungicide, nematicide, bactericide, herbicide, plant extract, plant growth regulator or fertilizer . 如請求項103之組合物，其中該微生物包含真菌物種。 The composition of claim 103, wherein the microorganism comprises a fungal species. 如請求項103之組合物，其中該微生物包含細菌物種。 The composition of claim 103, wherein the microorganism comprises a bacterial species. 如請求項103之組合物，其中該微生物包含芽孢桿菌屬微生物。 The composition of claim 103, wherein the microorganism comprises a microorganism of the genus Bacillus. 如請求項103之組合物，其中該微生物包含解澱粉芽孢桿菌。 The composition of claim 103, wherein the microorganism comprises Bacillus amyloliquefaciens.
TW105115451A 2016-05-18 2016-05-18 Bacillus amyloliquefaciens RTI472 compositions and methods of use for benefiting plant growth and treating plant disease TW201740808A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112029693A (en) * 2020-09-30 2020-12-04 河南省农业科学院植物保护研究所 Bacillus amyloliquefaciens with growth promoting effect and capable of preventing and treating sesame stem blight and fusarium wilt and application thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112029693A (en) * 2020-09-30 2020-12-04 河南省农业科学院植物保护研究所 Bacillus amyloliquefaciens with growth promoting effect and capable of preventing and treating sesame stem blight and fusarium wilt and application thereof
CN112029693B (en) * 2020-09-30 2022-06-07 河南省农业科学院植物保护研究所 Bacillus amyloliquefaciens with growth promoting effect and capable of preventing and treating sesame stem blight and fusarium wilt and application thereof

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