201124712 六、發明說明： 【發明所屬之技術領域】 本案係關於一種量測流體中懸浮微粒與其他物質特性的 方法，尤其，本案係關於一種先透過近紅外光量測出流體中 的懸浮微粒濃度，以扣除懸浮微粒對吸收光譜的影響’來定 性及定量流體中其他物質濃度之方法。 【先前技術】 水及廢水處理是人類在利用水資源的過程中無可迴避與 忽視的處理程序，但為一種耗費大量人力、物力以及財力資 源的程序。因此，即時獲取水及廢水的水質成份及其濃度等 資訊，以提供即時、完整且有效的控制策略，來提升廢水處 理系統之穩定性以及處理效率，已成為目前的研究重點。 目前即時水質監測技術有光學頻譜分析量測、Software sensor、人工智慧及統計等技術。其中利用光譜分析量測技 術可取代多數水質分析實驗與傳統接觸式量測設備，以減輕 分析時間過長與設備維護費用昂貴的問題。光譜分析量測技 術在廢水水質檢測上大多以紫外光/可見光區段分光作為.普 遍運用之方法，再搭配硝化或藥品添加等方法，可量測化學 需氧量、總有機碳、硝酸鹽類、總氮、金屬錯合物、懸浮微 粒等水質項目。 現今的光譜分析量測技術多以吸收光譜為主。廢水中溶 解性物質多以分子形態存在，特定波長的能量通過分子時會 改I鍵結電子的能階，光能莖會因此被吸收，使光譜上產生 吸收峰，因此不同物質的分子、原子和特定官能基^發光團 會造成吸收光譜的差異，可利用光譜特定波長來鑑定有機物 201124712 及其結構，達到定性廢水成分的目的。 匕卜及收的強度可以以兩個經驗公式來描述，Lambert’s L aw係指光被吸收的程度與來源有關 ，Beer’s Law則是描述 吸收作用和°及收分子的量呈正比，综合兩者而得 Beer Lambert定律，並用於含有數個不相互作用的物質之溶 液時，則可得到下列公式： ^totai = Aj + A2 + A3 +......+ An =fljZjCj + a2bc2 + a3bc3 + .··... + anbcn % 其中A為吸光度，a為莫耳吸光係數(L/mol.cm)，b為光 位長度(cm)，c為溶液濃度(m〇1/L)。透過上述公式以及定性 分析出的結果’即可定量出數個不相互作用的物質各自的濃 度。 然而水中懸浮微粒之吸收光譜，主要是散射作用所致， 散射量大小則與其顆粒表面積、表面性質、顆粒尺寸以及濃 度有關。其光散射將會阻擋光的前行而產生吸收值，使得其 他物質解析不易’無法有效定性與定量廢水成分。另外量測 φ 懸浮微粒之方法，目前僅有水樣過濾的方式，但此方法需要 人工採樣分析，不僅耗費人力及物力’並且無法即時獲取懸 浮微粒濃度的資訊。 本案申請人鑑於習知技術中的不足，經過悉心試驗與研 究’並一本鍥而不捨之精神，終構思出本案「一種量測流體 中懸浮微粒與其他物質特性的方法」，以吸收光譜量測技術， 進一步解析懸浮微粒的顆粒尺寸以及濃度與不同波長吸光度 的關係，可將廢水中的懸浮微粒定量後’比對已建立好的資 料庫’即可在扣除懸浮微粒吸光度的情況下，比對出其他物 質的濃度，以改善懸浮微粒干擾光譜分析的問題。 201124712 【發明内容】 為了克服懸浮微粒所提供的吸光度對於流體中物質濃度 解析的困難，本發明以吸收光譜量測技術，首先從近紅外光 吸光度判斷流體中是否存在懸浮微粒，再進一步藉由懸浮微 粒於紫外光與可見光波段之吸收波峰偏移之特性，透過懸浮 微粒顆粒尺寸與吸收波峰之波長的關係而獲得懸浮微粒的顆 粒尺寸。透過其已知的顆粒尺寸，再解析只有懸浮微粒會有 吸收峰的近紅外光波段，透過懸浮微粒濃度與吸光度的關係 而獲得懸浮微粒濃度，最後透過該懸浮微粒濃度以及該顆粒 尺寸，即可扣除懸浮微粒對整體吸光度的影響，進而能有效 的分析流體中其他成分與其濃度值。 為了達到上述目的，本發明提出一種量測一流體中懸浮 微粒濃度的方法，該方法包括下列步驟：（a)量測該流體的一 近紅外光光譜；（b)提供一第一參考資訊；以及(c)比對該近紅 外光光譜以及該第一參考資訊，量測出該流體中的該懸浮微 粒濃度。 根據上述構想，該步驟(a)更包括一步驟(al)排除超過該 近紅外光光譜的偵測極限之數據及低於該近紅外光光譜的偵 測極限之雜訊。 根據上述構想，該第一參考資訊為該近紅外光光譜的吸 光度與該懸浮微粒濃度以及懸浮微粒粒徑尺寸的關係。 根據上述構想，該流體還包括複數物質，且該步驟(c) 更包括下列步驟：（cl)量測該流體的一吸收光譜；（c2)提供一 第二參考資訊；（c3)比對該第二參考資訊以及該懸浮微粒濃 度，計算出該懸浮微粒對於該吸收光譜的一影響值；以及(c4) 將該吸收光譜之數據減去該影響值後，比對該第二參考資 201124712 訊’量測該複數物質之濃度。 根據上述構想，該影響值為該懸浮微粒之吸光度，而該 第二參考資訊包括該懸浮微粒濃度與該懸浮微粒之吸光度之 間的一第一關聯；以及該複數物質之濃度與該複數物質之吸 光度之間的一第二關聯。 本發明另提出一種量測一流體中複數顆粒的尺寸的方 法，忒方法包括下列步驟··（a)量測該流體的一光譜資訊；（b)201124712 VI. Description of the invention: [Technical field to which the invention pertains] The present invention relates to a method for measuring the characteristics of suspended particles and other substances in a fluid, in particular, the present invention relates to a method for measuring the concentration of suspended particles in a fluid by passing near-infrared light. , to deduct the effect of aerosols on the absorption spectrum' to characterize and quantify the concentration of other substances in the fluid. [Prior Art] Water and wastewater treatment is an unavoidable and neglected process in the process of using water resources, but it is a process that consumes a lot of manpower, material resources and financial resources. Therefore, it is the current research focus to obtain instant information on water quality and concentration of water and wastewater to provide immediate, complete and effective control strategies to improve the stability and treatment efficiency of wastewater treatment systems. At present, the instant water quality monitoring technology includes optical spectrum analysis measurement, software sensor, artificial intelligence and statistics. Among them, spectral analysis and measurement technology can replace most water quality analysis experiments and traditional contact measurement equipment to reduce the problem of excessive analysis time and expensive equipment maintenance. Spectral analysis and measurement technology mostly uses ultraviolet/visible section spectrophotometry as the method of universal application, and can be used to measure chemical oxygen demand, total organic carbon and nitrate in combination with nitrification or drug addition. Water quality projects such as total nitrogen, metal complexes and suspended particulates. Today's spectral analysis measurement techniques are mostly based on absorption spectra. The dissolved substances in the wastewater are mostly in the form of molecules. When the energy of a specific wavelength passes through the molecule, the energy level of the electrons is changed. The light energy stems are absorbed, and the absorption peaks are generated in the spectrum. Therefore, molecules and atoms of different substances are generated. And the specific functional group luminescence group will cause the difference of absorption spectrum, and the specific wavelength of the spectrum can be used to identify the organic matter 201124712 and its structure to achieve the purpose of qualitative wastewater components. The intensity of 匕 及 收 收 可以 可以 可以 La La La La La La La La La La La La La La La La La La La La La La La La La La La La La La La La La La La La La La La La La La La La La La La La When Beer Lambert's law is obtained and used for solutions containing several non-interacting substances, the following formula can be obtained: ^totai = Aj + A2 + A3 + ... + An = fljZjCj + a2bc2 + a3bc3 + . ··... + anbcn % where A is the absorbance, a is the molar absorption coefficient (L/mol.cm), b is the light length (cm), and c is the solution concentration (m〇1/L). Through the above formula and the qualitative analysis of the results, the concentration of each of the several non-interacting substances can be quantified. However, the absorption spectrum of suspended particles in water is mainly caused by scattering, and the amount of scattering is related to its particle surface area, surface properties, particle size and concentration. Its light scattering will block the light from moving forward and produce absorption values, making other material analysis difficult. It is not possible to effectively characterize and quantify wastewater components. In addition, the method of measuring φ suspended particles is currently only a method of filtering water samples, but this method requires manual sampling analysis, which not only consumes manpower and material resources, but also does not provide instant information on the concentration of suspended particles. In view of the deficiencies in the prior art, the applicant of this case, through careful experimentation and research, and the spirit of perseverance, finally conceived the case "a method for measuring the characteristics of suspended particles and other substances in fluids" to absorb spectral measurement technology. Further analyzing the particle size of the suspended particles and the relationship between the concentration and the absorbance at different wavelengths, the suspended particles in the wastewater can be quantified and 'aligned with the established database', and the absorbance of the suspended particles can be subtracted. Concentrations of other substances to improve the interference of suspended particles interfere with spectral analysis. 201124712 SUMMARY OF THE INVENTION In order to overcome the difficulty of analyzing the concentration of a substance in a fluid by the absorbance provided by the suspended particles, the present invention firstly uses the absorption spectrum measurement technique to first determine whether there are suspended particles in the fluid from the near-infrared light absorbance, and further suspends by suspension. The characteristic of the absorption peak shift of the particles in the ultraviolet and visible light bands, the particle size of the suspended particles is obtained by the relationship between the size of the suspended particles and the wavelength of the absorption peak. Through the known particle size, the near-infrared band of only the suspended particles has an absorption peak, and the concentration of the suspended particles is obtained by the relationship between the concentration of the suspended particles and the absorbance. Finally, the concentration of the suspended particles and the particle size can be The effect of aerosol on the overall absorbance is deducted, which in turn can effectively analyze other components in the fluid and their concentration values. In order to achieve the above object, the present invention provides a method for measuring the concentration of suspended particles in a fluid, the method comprising the steps of: (a) measuring a near-infrared spectrum of the fluid; (b) providing a first reference information; And (c) measuring the concentration of the aerosol in the fluid by comparing the near-infrared spectrum and the first reference information. According to the above concept, the step (a) further comprises a step (al) of eliminating data exceeding the detection limit of the near-infrared spectrum and noise below the detection limit of the near-infrared spectrum. According to the above concept, the first reference information is the relationship between the absorbance of the near-infrared light spectrum and the concentration of the suspended particles and the particle size of the suspended particles. According to the above concept, the fluid further comprises a plurality of substances, and the step (c) further comprises the steps of: (cl) measuring an absorption spectrum of the fluid; (c2) providing a second reference information; (c3) comparing the a second reference information and the concentration of the aerosol, calculating an influence value of the suspended particle on the absorption spectrum; and (c4) subtracting the influence value from the data of the absorption spectrum, compared with the second reference amount 201124712 'Measure the concentration of the complex substance. According to the above concept, the influence value is the absorbance of the suspended particles, and the second reference information includes a first correlation between the concentration of the suspended particles and the absorbance of the suspended particles; and the concentration of the plurality of substances and the plurality of substances A second association between absorbances. The present invention further provides a method of measuring the size of a plurality of particles in a fluid, the method comprising the steps of: (a) measuring a spectral information of the fluid; (b)
，供參考=貝訊，以及(C)比對該參考資訊以及該光譜資訊， 量測該複數顆粒的尺寸。 、，二=據上述構想，該步驟⑷更包括一步驟（al)排除超過該 光譜資訊的_極限之數據及低於絲譜資訊的偵測極限之 雜訊。 一根據上述構想，該參考資訊為該光譜資訊的吸收波峰之 與該複數顆粒的尺寸的關係，且該光譜資料包括一吸 尺十述構想’吸收波峰的驗長會隨著該複數顆粒的 尺寸變大而往長波長偏移。 數懸述?想’該流體包括一水體’該複數顆粒包括複 資訊中的、Η 4步驟⑻更包含下列步驟：（a2)確認該光譜 該吸光皮段之吸光度是否比純水高；以及(a3)若 光度與::相同：表表;::體具有該複數懸浮微粒’若吸 本2表不水體不具有該複數懸浮微粒。 質的濃度的流體中複數懸浮微粒與複數物 光譜資訊；__ //=下列辣：⑷量卿流體的一 數顆粒尺寸、一近紅外光光譜以及該參考資訊，計算 7 201124712 該流體中一懸浮微粒濃度；以及(d)利用該懸浮微粒濃度、 該複數顆粒尺寸、該光譜資訊以及該參考資訊，確定該流體 中該複數物質的一濃度資訊。 根據上述構想，該光譜資料包括該近紅外光光譜、一可 見光光譜以及一紫外光光譜，且該步驟(b)更包括下列步驟： (bl)透過該近紅外光光譜，確認該流體中是否具有該懸浮微 粒；以及(b2)透過該參考資訊以及該光譜資訊，取得該懸浮 微粒的該複數顆粒尺寸。 根據上述構想，該步驟(d)更包括下列步驟：（dl)利用該 懸浮微粒濃度、該複數顆粒尺寸以及該參考資訊，計算出該 流體中該懸浮微粒所提供的一第一吸光度；（d2)利用該第一 吸光度以及該光譜資訊，計算出該光譜資訊之數據減去該第 一吸光度後的一校正光譜；以及（d3)利用該校正光譜以及該 參考資訊，計算出該複數物質濃度。 根據上述構想，該參考資訊包括：該光譜資訊中該懸浮 微粒吸收波峰之一波長與該複數顆粒尺寸的關係；該光譜資 訊中吸光度與該懸浮微粒濃度以及該複數顆粒尺寸的關係； 以及該濃度資訊與該複數物質的吸光度之間的關係。 根據上述構想，該光譜資料包含一吸光度資訊以及一吸 收峰資訊的一吸收光譜。 【實施方式】 本案所提出之「一種量測流體中懸浮微粒與其他物質特 性的方法」將可由以下的實施例說明而得到充分暸解，使得 熟習本技藝之人士可以據以完成之，然而本案之實施並非可 由下列實施例而被限制其實施型態，熟習本技藝之人士仍可 201124712 依據除既揭露之實施例的精神推演出其他實施例， 例皆當屬於本發明之範圍。 本發明在進行光譜分析時，需要透過與一參考資訊的比 對，才能產生所需的結果。該參考資訊是由複數個吸收光譜 组成的資料庫所建立，該複數個吸收光譜包括一流體具有不 同懸浮微粒濃度與不同懸浮微粒顆粒尺寸的吸收光譜，'以及 具有不同濃度之其他物質的吸收光譜。 本發明提供了建立該流體中懸浮微粒濃度以及顆粒尺寸 與吸收光譜間的資料庫之一實施例1，該實施例i中使用了 如表一的顆粒尺寸與濃度，來探討其對於吸收光譜所產生的 影響。 表一：光譜資料庫之懸浮微粒之粒徑（/zm)與不同濃度(mg/L) 之顆粒數(單位:十億顆） 0.1 0.2 0.34 0.5 0.8 1.0 1.6 2.0 10 9099 1137 232 73 18 9.1 2.2 1.1 20 18198 2275 463 146 36 18 4.4 2.3 30 27298 3412 695 218 53 27 6.7 3.4 40 36397 4550 926 291 71 36 8.9 4.5 50 45496 5687 1158 364 89 45 11 5.7 60 54595 6824 1389 437 107 54 13 6.8 70 63694 7962 1621 510 124 64 16 8.0 80 72793 9099 1852 582 142 73 18 9.1 90 81893 10237 2084 655 160 82 20 10 100 90992 11374 2315 728 178 91 22 11 請參閱第一圖（A)，為該實施例1中，粒徑為Ι.Ο/zm的 9 201124712 懸洋微粒在不同濃度下的吸收光譜圖，隨著m粒的濃度 增加，該流體的吸光度會上升。請參㈣_圖⑻，為該 實施例1中，粒徑為的懸浮微粒在不同濃度與吸光度 的關係圖’其巾在紫外光、可見絲及近紅外紐段各選一 波長354 nni、500 nm以及978nm作為代表來分析。由圖可 以發現，在固定粒徑大小以及@定波長的情灯，該流體的 吸光度會隨著濃度的增加而線性遞增。請參㈣—圖(〇,為 邊實施例1中，懸浮微粒在不同粒徑與不同濃度下，對於波 ^為500 nm的可見光之吸光值。由圖可以發現在固定波長 日、，吸光度皆會隨濃度增加而增加，表示不同粒徑之顆粒其 吸光值與濃度的趨勢線斜率皆為線性關係，即可知曉該流體 中懸浮微粒對於吸光度的影響。 在本發明中，以同樣的方式建立其他成分的資料庫發 現在近紅外光的波段僅有懸浮微粒為因本身濃度的不同， 而景> 響該流體整體在近紅外光波段的吸光度。有機物與金屬 離子分別以鄰苯二甲酸鈉以及鉻離子(Cr3+)為例，請參閱第二 圖（A)以及第二圖（B)分別為鄰苯二曱酸鈉以及鉻離子（Cr3+) 在不同濃度下的光譜圖，比較第一圖（A)、第二圖（A)以及第 二圖（B)可以發現’近紅外光吸光度只會隨著懸浮微粒濃度的 増加而上升，但是吸光度並不會隨著金屬離子的鉻離子(Cr3+) 以及有機物的鄰苯二甲酸鈉之濃度增加而上升，而會維持純 水之吸光度。因此本發明可透過近紅外光波段的吸光度只受 懸浮微粒濃度以及粒徑影響的特性，可先利用近紅外光譜解 析有關懸浮微粒濃度以及粒徑的資訊。 事實上，一流體的懸浮微粒濃度以及粒徑通常皆為未 知’若要知道懸浮微粒對於該流體吸光度的影響，必須先確 201124712 定懸浮微粒濃度及粒#，再比對資料庫才可得知。請參閱第 三圖（A)為相同顆粒數的懸浮微粒在不同粒徑下的光譜圖，由 於粒徑大小不同，因此該流體中的懸浮微粒濃度會有曰所不同 (’J示於表―），導致顆粒尺寸越大而懸浮微粒濃度越高，所 以吸光度越高。此外由圖可以發現在紫外光與可見光的波 段，懸洋微粒吸收峰的波長會因懸浮微粒的顆粒尺寸變大， 而往較長的波長移動。請與第—圖比較可發現，在相同顆粒 尺寸不同懸浮微粒濃度的情況下，吸收峰並不會因激度的改For reference = Beixun, and (C) to measure the size of the plurality of particles compared to the reference information and the spectral information. According to the above concept, the step (4) further includes a step (al) for excluding data exceeding the _limit of the spectral information and noise below the detection limit of the spectral information. According to the above concept, the reference information is the relationship between the absorption peak of the spectral information and the size of the complex particle, and the spectral data includes a suction ruler. The length of the absorption peak will vary with the size of the complex particle. It becomes larger and shifts to longer wavelengths. The number of suspensions? I think 'the fluid includes a body of water', the plurality of particles including the complex information, Η 4 step (8) further comprises the following steps: (a2) confirm whether the absorbance of the spectrum of the light absorption section is higher than pure water; A3) If the luminosity is the same as:: table;:: the body has the plurality of suspended particles 'If the sample 2 does not have the plurality of suspended particles. Spectral information of a plurality of suspended particles and complex matter in a mass concentration; __ //= the following spicy: (4) a particle size of a quantity of a fluid, a near-infrared spectrum, and the reference information, calculation 7 201124712 a suspension in the fluid a concentration of the particles; and (d) determining a concentration information of the plurality of substances in the fluid using the concentration of the suspended particles, the size of the plurality of particles, the spectral information, and the reference information. According to the above concept, the spectral data includes the near-infrared spectrum, a visible spectrum, and an ultraviolet spectrum, and the step (b) further includes the following steps: (bl) confirming whether the fluid has the fluid through the near-infrared spectrum The suspended particles; and (b2) obtaining the plurality of particle sizes of the suspended particles through the reference information and the spectral information. According to the above concept, the step (d) further comprises the steps of: (dl) calculating a first absorbance provided by the suspended particles in the fluid by using the suspended particle concentration, the plurality of particle sizes, and the reference information; (d2) Using the first absorbance and the spectral information, calculating a corrected spectrum of the data of the spectral information minus the first absorbance; and (d3) calculating the concentration of the plurality of substances using the corrected spectrum and the reference information. According to the above concept, the reference information includes: a relationship between a wavelength of the absorption peak of the suspended particle and the size of the complex particle in the spectral information; a relationship between the absorbance of the spectral information and the concentration of the suspended particle and the size of the complex particle; The relationship between information and the absorbance of the plural substance. According to the above concept, the spectral data contains an absorbance information and an absorption spectrum of an absorption peak information. [Embodiment] The "method of measuring the characteristics of suspended particles and other substances in a fluid" proposed in the present invention will be fully understood by the following examples, so that those skilled in the art can accomplish this, but the present case The implementations are not limited by the following embodiments, and those skilled in the art can still use the embodiments of the present invention in addition to the embodiments disclosed herein. In the spectral analysis of the present invention, it is necessary to compare with a reference information to produce a desired result. The reference information is established by a database consisting of a plurality of absorption spectra including an absorption spectrum of a fluid having different aerosol concentrations and different aerosol particle sizes, and an absorption spectrum of other substances having different concentrations. . The present invention provides an embodiment 1 for establishing a concentration of suspended particles in the fluid and a particle size and absorption spectrum. In this example i, the particle size and concentration as shown in Table 1 are used to investigate the absorption spectrum. The impact. Table 1: Particle size (/zm) and particle size (mg/L) of suspended particles in the spectral database (unit: billion) 0.1 0.2 0.34 0.5 0.8 1.0 1.6 2.0 10 9099 1137 232 73 18 9.1 2.2 1.1 20 18198 2275 463 146 36 18 4.4 2.3 30 27298 3412 695 218 53 27 6.7 3.4 40 36397 4550 926 291 71 36 8.9 4.5 50 45496 5687 1158 364 89 45 11 5.7 60 54595 6824 1389 437 107 54 13 6.8 70 63694 7962 1621 510 124 64 16 8.0 80 72793 9099 1852 582 142 73 18 9.1 90 81893 10237 2084 655 160 82 20 10 100 90992 11374 2315 728 178 91 22 11 Please refer to the first figure (A) for the particle size of this example 1. For the absorption spectrum of 9 201124712 suspension particles of Ι.Ο/zm at different concentrations, as the concentration of m particles increases, the absorbance of the fluid will increase. Please refer to (4)_Fig. (8), which is the relationship between the concentration and the absorbance of the suspended particles with particle size in the first embodiment. The towel has a wavelength of 354 nni, 500 in the ultraviolet, visible and near-infrared sections. Nm and 978 nm were analyzed as representatives. It can be seen from the figure that at a fixed particle size and a constant wavelength, the absorbance of the fluid increases linearly with increasing concentration. Please refer to (4)-Fig. (〇, for the side-by-side example, the absorbance of visible light for 500 μm of suspended particles at different particle sizes and different concentrations. It can be found at fixed wavelength days, absorbance It will increase with the increase of the concentration, indicating that the particles of different particle sizes have a linear relationship between the absorbance value and the trend line slope of the concentration, so that the influence of the suspended particles in the fluid on the absorbance can be known. In the present invention, the same manner is established. The database of other components found that only the suspended particles in the near-infrared light band are due to their respective concentrations, and the sound of the fluid is in the near-infrared band. The organic matter and the metal ions are sodium phthalate and For example, chrome ion (Cr3+), please refer to the second graph (A) and the second graph (B) for the spectra of sodium phthalate and chromium ions (Cr3+) at different concentrations, respectively. A), the second figure (A) and the second figure (B) can be found that 'near-infrared light absorbance only rises with the increase of the concentration of suspended particles, but the absorbance does not follow the chromium ion of the metal ion (Cr 3+) and the concentration of organic sodium phthalate increase and increase the absorbance of pure water. Therefore, the absorbance of the present invention through the near-infrared light band is affected only by the concentration of suspended particles and the particle size, and can be utilized first. Near-infrared spectroscopy analyzes information about aerosol concentration and particle size. In fact, the concentration and particle size of a fluid suspension are usually unknown. To know the effect of aerosol on the absorbance of the fluid, it is necessary to confirm the 201114312 aerosol. Concentration and particle #, and then compare the database can be known. Please refer to the third figure (A) is the spectrum of the same particle number of suspended particles in different particle sizes, due to the different particle size, so in the fluid The concentration of suspended particles will be different ('J is shown in Table-), resulting in larger particle size and higher concentration of suspended particles, so the higher the absorbance. In addition, the particles can be found in the ultraviolet and visible light bands, suspended particles The wavelength of the absorption peak will shift to a longer wavelength due to the larger particle size of the suspended particles. Please compare with the first image to find that the same In the case where the particle size is different from the concentration of suspended particles, the absorption peak is not changed by the sensitivity.
變有所偏移。因此斷定第三圖⑷中’該吸收波峰的偏移是受 到粒徑的影響。 110 1.6 2 450 879 表二 粒徑（ym) 0.1 0.2 0.34 r 0.5 濃度(mg/L) 0.1 1 4 14 0.8 56 叫不一 _、以砷个M祖桎興吸收波峰的波長之關 係。由圖可看出，紫外光與可見光波段吸收峰的前三個吸收 波峰皆會隨著齡尺寸的增加而往長波長偏移。因此，可透 過紫外光與可見光波段的吸收峰之波長，來確定該流體中懸 浮微粒的肺尺寸’並藉由已知的齡尺寸再輯近紅外光 波段的吸光度，即可1：測出職财料微粒濃度，最後比 對懸浮微粒濃度以及顆粒尺寸，即可量測出懸浮微粒對於該 流體所提供的吸光度。 μ參閱第四圖，本發明提供—種量測—流體中複數懸浮 微粒與複數物質的濃度的-實施例2,其步驟包括：（s41)提 供-流體；（S42)量測該流體的一光譜資訊，該光譜資訊包 括-近紅外光光譜以及-紫外/可見光光譜；（S43)提供作為 201124712 一參考值的資料庫；（S44)透過近紅外光光譜確認是否有複 數懸浮微粒；（S45)透過該參考值以及該紫外/可見光光議， 量測該懸浮微粒的複數顆粒尺寸；（S46)利用該流體中^複 數顆粒尺寸、該近紅外光光譜以及該參考值，計算該流體中 一懸浮微粒濃度；（S47)利用該懸浮微粒濃度、該複數顆粒 尺寸以及該參考值’確定該流體中該懸浮微粒所提供的一第 一吸光度；（S48)將該光譜資訊扣除該懸浮微粒所提供得誃 第一吸光度，計算出一校正光譜；（S49)透過該校正光譜^ 及該參考值計算出該流體中複數物質的濃度。此外在^驟 S45確認並無該懸浮微粒時，則將該光譜資訊直接視為該校 鲁 正光譜，並透過該參考值來計算該複數物質的濃度。 該實施例2中可應用於廢水分析。可將一廢水(步驟S4i) 先經過例如分光光度計等光譜分析儀器，該廢水經過近紅外 光、可見光以及紫外光照射後，會有特定波長的光被廢水所 吸收或散射，而產生一個作為光譜資訊的吸收光譜（步驟 S42)，該吸收光譜會顯示不同波長所具有得不同吸光度。提 供一個資料庫’該資料庫包括純水的吸收光譜、僅有懸浮微 粒的溶液之吸收光谱以及其他單一成份的溶液之吸收光譜 鲁 (步驟S43) ’比較純水以及該廢水的吸收光譜，透過近紅外光 波段中該廢水的吸光度與純水的吸光度是否相同，來確認該 廢水中是否具有複數懸浮微粒（步驟S44)。 若該廢水在近紅外光波段的吸光度高於純水(步驟S44的 「是」），則表示該廢水具有懸浮微粒。利用紫外/可見光波 丰又δ亥廢水的吸收波峰的波長，比對僅有懸浮微粒的水體之吸 收光譜，即可量測出該廢水之懸浮微粒的複數顆粒尺寸（步驟 S45) ’再利用近紅外光對於該廢水的吸光度以及該複數顆粒 12 201124712 尺寸’比對僅有懸浮微粒的水體之吸收光譜，即可量測出該 廢水的懸浮微粒濃度(步驟S46)’由於懸浮微粒的該複數顆粒 尺寸及濃度確定後’即可比對資料庫的資料，而知道該廢水 中懸浮微粒在不同波段所提供的吸光度（步驟S 4 7 )，將該廢水 的吸收光譜扣除懸浮微粒所提供的吸光度，可計算出該廢水 若移除懸浮微粒後的一個作為校正光譜的虛擬吸收光譜（步 驟S48)，該校正光譜的吸光度除了純水所提供的吸光度外， 即為作為該複數物質的其他物質所提供的吸光度，可再透過 二貝料庫的比對，經由各種運算法來定性定量該廢水中所有物 質的成份以及濃度（步驟S49)。 ^該廢水在近紅外光波段的吸光度與純水相同（步驟S44 的「否」），即表示該廢水中並無懸浮微粒，因此該光譜資訊 並不受懸浮微粒的干擾，意即該光譜資訊就是已扣除懸浮微 粒干擾的該校正光譜，可透過該校正光譜以及該參考值來定 性定量定性定量該廢水中所有物質的成份以及濃度（步驟 S49)。 在上述實施例的應用中’做為該參考值的資料庫，可具 有下列五種功能，（1)從資料庫中獲得純水的吸收光讀，比對 該廢水的吸收光譜，從近紅外光的吸收值大小確定該廢水中 是否有懸浮微粒(步驟S44) ; (2)從資料庫中獲取不同懸浮微 粒該複數顆粒尺寸的吸收波峰之波長，比對該廢水的紫外/ 可見光吸收波峰，以確定懸浮微粒的該複數顆粒尺寸（步驟 S45) ; (3)從資料庫中獲知該複數顆粒尺寸的不同懸浮微粒濃 度之吸光度，從近紅外光波段的吸光度比對出懸浮微粒的濃 度(步驟S46) ; (4)就已知的該複數顆粒尺寸以及濃度，從資 料庫中得出該廢水中懸浮微粒所提供的吸光度(步驟S47);及 13 201124712 該，譜的吸收波峰 質的漢度(步驟S49)。 讀及定量出各種物 在該實施例2中，丰跡C/10 @、条 並定量該流體中該複數=:度需透===性 比對該廢水之吸收光議 參考，、步驟包括⑻ 日及資枓庫，推估各波長可能存在之 欢—S"廢水吸收光譜，是否有波峰戋波谷盥 成分相符，若有 與資枓庫 ㈣成八的:、斷5亥廢水3有該吸收成分）；⑻選取 及收成》的主要吸收峰，該波 的吸光係數，計算推估心，料管&.吸光度與該成刀 度的差值，若多成八/ 農算推估吸光度與實際吸光 重新推估吸Μ :有差值為負之狀況’則取負值最大者 的έ士里4 W丨又直至差值全為正為止’即可獲得定性分析 旦:光譜特性㈣庫，確認各成份間是否會相互 =日。右交互衫響則比對資料庫，將該影響還原產生一還 :、光a' (d)若。魄原光譜具有單—吸收成分的連續波段，以 Ϊ較長者優先’依序檢録估濃度(任選-職波長，根據 貫際吸光度以及吸㈣數，計算出修正濃度，輕該修正濃 度之吸光度與實際吸光度差值全為負為止）；（e)若該還原光譜 具有兩個吸收成分的連段，續段較長者優先，依序檢 查是f包含已計算過修正濃度的成分，若有，則參照步驟d 去計算；若無，則利用線性規劃求解的方式修正兩個成分的 推估濃度(利用該連續波段的每—波長，計算兩成份推估值的 加 '、息吸光度與貫際吸光度，以差值最大的波長及其相鄰四個 1長進行線性規劃，再以最小化差值最大的波長之差值為目 標函數規劃求解，最後將所得修正濃度重新計算吸光度差 201124712 值，以確認最大吸光度差值小於分光光度計的偵測雜訊為 止）；（f)若該還原光譜具有三個吸收成分的連續波段，以波段 較長者優先，依序檢查是否包含已計算過修正濃度的成分， 若有，則透過步驟d以及e去計算，若無，仍參照步驟e的 方式，利用線性規劃求得三個成分的推估濃度；（g)依據步驟 f的方式去檢查是否有四個以上吸收成分的連續波段，並進 行運算’直至所有吸收成分都已修正；及(h)檢查修正濃度的 加總吸光度是否與實際吸光度的差值小於偵測雜訊，若有大 擊 於雜訊之波段’則以步驟e的方式重新線性規劃來修正，直 至差值全部小於偵測雜訊’即完成定量分析的動作。 在該實施例2中，若步驟S42所獲得的吸收光譜存在超 過值測極限或小於價測雜訊的一無效吸光度時，則可於步驟 S42以及步驟S43之間***一步驟，以排除該無效吸光度， 以避免影響後續定性定量分析的運算。此外作為一參考值的 資料庫是一整個資料庫的内容之一參考資訊。 請參閱第五圖，本發明提供一種量測一流體中複數顆粒 φ 的尺寸的之一實施例4,其步驟包括：（S51)提供一流體；（S52) 量測該流體的一光譜資訊，該光譜資訊包括一近紅外光光譜 以及一紫外/可見光光譜；（S53)排除超過該光譜資訊的偵測 極限之數據及低於該光譜資訊的偵測極限之雜訊；（S54)提 $作為一參考值的資料庫；（S55)透過該近紅外光光譜確認 疋否有複數顆粒，（S56)透過該參考值以及該紫外/可見光光 譜，量測該複數顆粒的尺寸；及(S57)取得該複數顆叙的尺 寸。此外在步驟S55確認並無該複數顆粒時，則可視該複數 顆粒的尺寸為0。 該λ施例4中可應用於廢水分析。可將一廢水(步驟mi) 15 201124712 先經過例如分光光度計等光譜分析儀器，該廢水經過近紅外 光、可見光以及紫外光照射後，會有特定波長的光被廢水所 吸收或散射，而產生一個作為光譜資訊的吸收光譜（步驟 S52)，該吸收光譜會顯示不同波長所具有得不同吸光度，其 中可能會有一部分的吸光度超過該吸收光譜的偵測極限或低 於該吸收光譜的偵測極限，因此將不在偵測極限範圍内的數 據排除，以利後續分析（步驟S53)。提供一個資料庫，該資料 庫包括純水的吸收光谱以及僅有懸浮微粒的溶液之吸收光譜 (步驟S54)’比較純水以及該廢水的吸收光譜，透過近紅外光 波段中該廢水的吸光度與純水的吸光度是否相同，來確認該 廢水中是否具有作為該複數顆粒的懸浮微粒（步驟S55)。若該 廢水的吸光度高於純水(步驟S55的「是」），則表示該廢水 具有懸浮微粒。利用紫外/可見光波段該廢水的吸收波峰的波 長，比對僅有懸浮微粒的水體之吸收光譜，即可量測出該廢 水的懸浮微粒的複數顆粒尺寸（步驟S56及步驟S57)。 若比對該廢水的吸光度在近紅外光波段與純水相同，即 表示該廢水中無懸浮试粒(步驟§55的「否」），亦可視懸浮 微粒的顆粒尺寸為〇,而取得懸浮微粒的顆粒尺寸（步驟S57)。 在上述實施例的應用中，做為該參考值的資料庫，可具 有下列兩種功能’（1)從資料庫中獲得純水的吸收光譜，比對 該廢水的吸收光譜，從近紅外光的吸收值大小，以確定該廢 水中是否有懸浮微粒（步驟S55);及(2)從資料庫中獲取不同 懸浮微粒顆粒尺寸的吸收波峰之波長，來比對該廢水的紫外/ 可見光吸收波峰，以確定懸浮微粒的該複數顆粒尺寸（步驟 S56)。 在步驟S56中，由資料庫可以發現當懸浮微粒的該複數 201124712Changed to some offset. Therefore, it is concluded that the shift of the absorption peak in the third graph (4) is affected by the particle diameter. 110 1.6 2 450 879 Table 2 Particle size (ym) 0.1 0.2 0.34 r 0.5 Concentration (mg/L) 0.1 1 4 14 0.8 56 It is not the same as _, the relationship between the wavelengths of the absorption peaks of arsenic M. It can be seen from the figure that the first three absorption peaks of the absorption peaks in the ultraviolet and visible light bands are shifted to long wavelengths as the age increases. Therefore, the lung size of the suspended particles in the fluid can be determined by the wavelength of the absorption peaks in the ultraviolet and visible light bands, and the absorbance of the near-infrared band can be recovered by the known age size. The particle concentration, and finally the concentration of the aerosol and the particle size, can be used to measure the absorbance provided by the aerosol for the fluid. Referring to the fourth figure, the present invention provides a method for measuring the concentration of a plurality of suspended particles and a plurality of substances in a fluid, the steps of which include: (s41) providing a fluid; (S42) measuring the fluid. Spectral information, the spectral information includes - near-infrared light spectrum and - ultraviolet / visible light spectrum; (S43) provides a reference database as 201124712; (S44) confirms whether there are multiple suspended particles through near-infrared light spectrum; (S45) Measuring, by the reference value and the ultraviolet/visible light, a plurality of particle sizes of the suspended particles; (S46) calculating a suspension in the fluid by using the complex particle size, the near-infrared light spectrum, and the reference value of the fluid a concentration of the particles; (S47) determining a first absorbance provided by the suspended particles in the fluid using the concentration of the suspended particles, the plurality of particle sizes, and the reference value; (S48) providing the spectral information by subtracting the suspended particles誃 First absorbance, a corrected spectrum is calculated; (S49) the concentration of the plurality of substances in the fluid is calculated through the corrected spectrum and the reference value. Further, when it is confirmed in S45 that the suspended particles are not present, the spectral information is directly regarded as the corrected positive spectrum, and the concentration of the complex substance is calculated by the reference value. This Example 2 can be applied to wastewater analysis. A waste water (step S4i) may be subjected to a spectroscopic analysis instrument such as a spectrophotometer. After the waste water is irradiated with near-infrared light, visible light, and ultraviolet light, light of a specific wavelength is absorbed or scattered by the waste water to generate a The absorption spectrum of the spectral information (step S42), which shows that the different wavelengths have different absorbances. Providing a database comprising an absorption spectrum of pure water, an absorption spectrum of a solution containing only suspended particles, and an absorption spectrum of a solution of other single components (step S43) 'Comparative to pure water and absorption spectrum of the wastewater, through Whether the absorbance of the wastewater in the near-infrared light band is the same as the absorbance of the pure water is to confirm whether or not the plurality of suspended particles are present in the wastewater (step S44). If the absorbance of the wastewater in the near-infrared light band is higher than that of pure water ("YES" in step S44), it means that the wastewater has suspended particles. By using the wavelength of the absorption peak of the ultraviolet/visible wave and the δHai wastewater, the absorption spectrum of the water having only the suspended particles can be measured, and the complex particle size of the suspended particles of the wastewater can be measured (step S45). The absorbance of the wastewater by the infrared light and the absorption spectrum of the water particle having only the suspended particles by the size of the plurality of particles 12 201124712, the concentration of the suspended particles of the wastewater can be measured (step S46) 'the plurality of particles due to the suspended particles After the size and concentration are determined, the data of the database can be compared, and the absorbance provided by the suspended particles in the different wavelength bands is known (step S 4 7 ), and the absorption spectrum of the wastewater is subtracted from the absorbance provided by the suspended particles. Calculating a virtual absorption spectrum as a corrected spectrum of the wastewater after removing the suspended particles (step S48), the absorbance of the corrected spectrum being provided by the other substances as the plural substance in addition to the absorbance provided by the pure water The absorbance can be further calibrated to the contents of the wastewater by various algorithms through the comparison of the two shells. And the concentration of component (step S49). ^ The absorbance of the wastewater in the near-infrared light band is the same as that of pure water ("No" in step S44), which means that there is no suspended particles in the wastewater, so the spectral information is not interfered by the suspended particles, that is, the spectral information That is, the corrected spectrum has been deducted from the interference of the suspended particles, and the calibration spectrum and the reference value are used to qualitatively and quantitatively quantify the composition and concentration of all substances in the wastewater (step S49). In the application of the above embodiment, the database as the reference value may have the following five functions: (1) obtaining absorption light reading of pure water from the database, compared to the absorption spectrum of the wastewater, from near infrared The absorption value of the light determines whether there is suspended particles in the wastewater (step S44); (2) obtaining the wavelength of the absorption peak of the plurality of particles from the database, which is higher than the ultraviolet/visible absorption peak of the wastewater, Determining the complex particle size of the suspended particles (step S45); (3) obtaining the absorbance of different suspended particle concentrations of the complex particle size from the database, and comparing the concentration of the suspended particles from the absorbance in the near-infrared band (step S46); (4) obtaining the absorbance provided by the suspended particles in the wastewater from the database according to the known particle size and concentration (step S47); and 13 201124712, the absorption peak of the spectrum (Step S49). Reading and quantifying various things in this embodiment 2, the trace C/10 @, the strip and quantifying the complex number in the fluid =: degree required to pass === sex ratio reference to the absorption of the wastewater, the steps include (8) Days and assets, estimate the possible existence of the wavelengths of each wavelength - S " wastewater absorption spectrum, whether there is a peak chopping wave 盥 盥 盥 , , , , , , , , , , , , 若 若 若 5 5 5 5 5 5 5 5 (8) Absorption component); (8) The main absorption peak of the selection and harvest, the absorption coefficient of the wave, calculate the difference between the estimated value of the heart, the tube & absorbance and the degree of formation, if more than eight / agronomic estimation of absorbance Re-estimate the sucking with the actual absorbance: if the difference is negative, then take the 4W in the gentleman with the largest negative value and until the difference is all positive. Then you can get the qualitative analysis: Spectral characteristics (4) , to confirm whether each component will mutually = day. The right interactive shirt is compared to the database, and the effect is restored to produce a return:, light a' (d) if. The spectrum of the original spectrum has a continuous band of single-absorption components, and the longer one is preferred. The concentration is determined in order (optional-operating wavelength, according to the continuous absorbance and the number of suction (four), the corrected concentration is calculated, and the corrected concentration is light. The difference between the absorbance and the actual absorbance is all negative); (e) if the reduction spectrum has a continuous segment of two absorption components, the longer segment is preferred, and the sequential check is that f contains the component with the corrected concentration calculated, if any , refer to step d to calculate; if not, use the linear programming solution to correct the estimated concentration of the two components (using the per-wavelength of the continuous band, calculate the addition of the two components, the absorbance and the absorption) The absorbance is linearly planned with the wavelength with the largest difference and its adjacent four lengths, and then the difference between the wavelengths that minimize the difference is used as the objective function to solve the solution. Finally, the corrected concentration is recalculated to calculate the absorbance difference 201124712. To confirm that the maximum absorbance difference is less than the detection noise of the spectrophotometer); (f) if the reduction spectrum has a continuous band of three absorption components, the band is longer Priority is given to check whether the components with the corrected concentration have been calculated, and if so, the calculation is performed through steps d and e. If not, the estimated concentration of the three components is obtained by linear programming with reference to the method of step e. (g) according to the method of step f to check whether there are more than four continuous components of the absorption component, and perform the operation 'until all the absorption components have been corrected; and (h) check whether the corrected absorbance of the corrected concentration and the actual absorbance The difference is less than the detection noise. If there is a big hit in the band of the noise, then re-linearly plan in the way of step e to correct until the difference is less than the detection noise, that is, the action of quantitative analysis is completed. In the second embodiment, if the absorption spectrum obtained in step S42 exceeds the value limit or is less than an invalid absorbance of the price measurement noise, a step may be inserted between step S42 and step S43 to exclude the invalidity. Absorbance, to avoid affecting the operation of subsequent qualitative and quantitative analysis. In addition, the database as a reference value is one of the contents of an entire database. Referring to FIG. 5, the present invention provides an embodiment 4 for measuring the size of a plurality of particles φ in a fluid, the steps comprising: (S51) providing a fluid; (S52) measuring a spectral information of the fluid, The spectral information includes a near-infrared spectrum and an ultraviolet/visible spectrum; (S53) excluding data exceeding the detection limit of the spectral information and noise below the detection limit of the spectral information; (S54) a reference value database; (S55) confirming whether there is a plurality of particles through the near-infrared light spectrum, (S56) measuring the size of the plurality of particles through the reference value and the ultraviolet/visible spectrum; and (S57) obtaining The size of the plural number. Further, when it is confirmed in step S55 that the plural particles are not present, it is considered that the size of the plural particles is zero. The λ Example 4 can be applied to wastewater analysis. A waste water (step mi) 15 201124712 may be subjected to a spectroscopic analysis instrument such as a spectrophotometer, and after the waste water is irradiated by near-infrared light, visible light, and ultraviolet light, light of a specific wavelength is absorbed or scattered by the waste water to generate An absorption spectrum as spectral information (step S52), which exhibits different absorbances at different wavelengths, wherein a portion of the absorbance may exceed the detection limit of the absorption spectrum or be below the detection limit of the absorption spectrum Therefore, the data that is not within the detection limit range is excluded to facilitate subsequent analysis (step S53). Providing a database comprising an absorption spectrum of pure water and an absorption spectrum of a solution containing only suspended particles (step S54) 'Comparatively pure water and an absorption spectrum of the wastewater, the absorbance of the wastewater in the near-infrared light band and Whether the absorbance of the pure water is the same or not, it is confirmed whether or not the waste water has the suspended particles as the plural particles (step S55). If the absorbance of the wastewater is higher than that of pure water ("YES" in step S55), it means that the wastewater has suspended particles. The complex particle size of the suspended particles of the waste water can be measured by measuring the wavelength of the absorption peak of the wastewater in the ultraviolet/visible band and comparing the absorption spectrum of the water having only the suspended particles (steps S56 and S57). If the absorbance of the wastewater is the same as that of the pure water in the near-infrared light band, it means that there is no suspended test sample in the wastewater ("No" in step §55), and the suspended particles can be obtained by using the particle size of the suspended particles as 〇. The particle size (step S57). In the application of the above embodiment, the database as the reference value may have the following two functions' (1) obtaining an absorption spectrum of pure water from the database, compared to the absorption spectrum of the wastewater, from near-infrared light. The absorption value is determined to determine whether there is aerosol in the wastewater (step S55); and (2) the wavelength of the absorption peak of the particle size of the different aerosol particles is obtained from the database to compare the ultraviolet/visible absorption peak of the wastewater. To determine the complex particle size of the aerosol (step S56). In step S56, the plural of the suspended particles can be found by the database 201124712
量測該流體的一 如乃提供一種量測一流體中懸浮微粒 例5，其步驟包括：（S61)提供一流體；（S62) 近紅外光光譜；（S63)排除超過該光譜資訊 的偵測極限之數據及低於該光譜資訊的情測極限之雜訊； (S64)提供作為—第—參考值的資料庫；及_)利用該近 紅外光光譜以及該第—參考值，計算誠體巾懸浮微粒濃度。 該實施例5巾可應用於廢水分析。可將已知懸浮微粒一 粒徑尺^的一廢水(步驟S61)先經過例如分光光度計等光譜 分析儀器，該廢水經過近紅外光照射後，會有特定波長的光 被廢水所吸收或散射’而產生—個近紅外光吸收光譜(步驟 S62)，該近紅外光吸收光譜會顯示不同波長所具有的不同吸 光度，其中可能會有一部分的吸光度超過該吸收光譜的偵測 極限或低於該吸收光譜的偵測極限，因此將不在偵測極限範 圍内的數據排除，以利後續分析(步驟S63)。提供一個作為第 一參考值的資料庫，該資料庫包括僅有懸浮微粒的水體之吸 收光譜(步驟S64)，再利用該粒徑尺寸，先找出與該粒徑尺寸 相同，但不同懸浮微粒濃度的吸收光譜，經比對後，即可量 測出該廢水的懸浮微粒濃度(步驟S65)。 在上述實施例的應用中’做為該參考值的資料庫，可從 其中獲取該近紅外光光譜的吸光度與該懸浮微粒濃度以及懸 浮微粒粒徑尺寸的關係，從中找出與該顆粒尺寸相同，但不 同懸浮微粒濃度的吸收光譜(步驟S65)。 17 201124712 在該實施例5應用於廢水分析時，可於取得懸浮微粒濃 度後，進一步定量該廢水中的複數物質，其步驟包括：量 測該θ廢水的—吸收賴，該吸收錢包括-紫外/可見光譜； (b)提供一個作為第二參考值的資料庫；（c)比對資料庫以及該 懸浮微粒濃度與粒徑尺寸’即可獲得該懸浮微粒對於該吸收 光谱的一影響值；及(d)將該吸收光譜之數據減去該影響值 後，即可比對資料庫，透過如實施例3的特定演算法去量測 該複數物質之濃度。 如上述實施例中，該影響值為該吸收光譜中該懸浮微粒 所提供的吸光度，透過減去該影響值，即可取得純水以及該 複數物質所提供的吸光度。此外作為一參考值的資料庫是一 整個資料庫的内容之一參考資訊。 如上述實施例中，作為該第二參考值的資料庫，可具有 下列兩種功能，（1)就已知的懸浮微粒粒徑尺寸以及濃度，從 資料庫中取得該廢水的該影響值；及⑺利用資料庫以^減去 遠影響值的光譜資訊’比對該複數物質的吸光度與吸收波 峰’來計算該複數物質的濃度。 以上所述實施例僅係為了方便說明而舉例，並非限 發明。因此熟悉本技藝之人士在不違背本發明之精神，對於 例進行修改、變化’然皆不脫如附申請專利範圍所 【圖式簡單說明】 第一圖⑷為本案實施合"以粒徑為1〇叫的 在不同濃度下的吸收光譜圖； 第-圖⑻為本案實施例〗以粒徑為1()帅㈣浮微粒在 201124712 不同濃度與吸光度的關係圖； 第一圖（c)為本案實施例1，懸浮微粒在不同粒徑與不同 濃度下，對於波長為500 nm的可見光之吸光值； 第二圖（A)為鄰苯二曱酸鈉在不同濃度下的光譜圖； 第二圖（B)為鉻離子(Cr3+)在不同濃度下的光譜圖； 第三圖（A)為相同顆粒數的懸浮微粒在不同粒徑下的光譜 圖； 第三圖（B)為不同顆粒尺寸與吸收波峰的波長之關係； 第四圖為本發明的一實施例2之流程圖； ® 第五圖為本發明的一實施例4之流程圖；以及 第六圖為本發明的一實施例5之流程圖。 【主要元件符號說明】 S41〜S49步驟 S51〜S57步驟 S61〜S65步驟 19Measuring the fluid as an example provides a method for measuring suspended particles in a fluid, the steps of which include: (S61) providing a fluid; (S62) near-infrared light spectrum; (S63) eliminating detection beyond the spectral information a limit data and a noise level lower than an emotional limit of the spectral information; (S64) providing a database as a - reference value; and _) calculating the honest body using the near-infrared light spectrum and the first reference value The concentration of the aerosol particles. This Example 5 towel can be applied to wastewater analysis. A wastewater having a known particle size-particle size (step S61) may be subjected to a spectroscopic analyzer such as a spectrophotometer, and the wastewater is absorbed or scattered by the wastewater after being irradiated by near-infrared light. 'produces a near-infrared light absorption spectrum (step S62), the near-infrared light absorption spectrum will display different absorbances of different wavelengths, wherein a part of the absorbance may exceed the detection limit of the absorption spectrum or lower than the The detection limit of the absorption spectrum is excluded, so that data not within the detection limit range is excluded for subsequent analysis (step S63). Providing a database as a first reference value, the database includes an absorption spectrum of a water body having only suspended particles (step S64), and then using the particle size to first find the same size as the particle size, but different aerosols The absorption spectrum of the concentration, after comparison, measures the concentration of suspended particles of the wastewater (step S65). In the application of the above embodiment, as a database of the reference value, the relationship between the absorbance of the near-infrared light spectrum and the concentration of the suspended particles and the particle size of the suspended particles can be obtained therefrom, and the same size as the particle can be found. However, the absorption spectra of different aerosol concentrations (step S65). 17 201124712 When the fifth embodiment is applied to the analysis of wastewater, the plurality of substances in the wastewater can be further quantified after the concentration of the suspended particles is obtained, and the steps include: measuring the absorption of the wastewater of the θ, the absorption including UV (b) providing a database as a second reference value; (c) comparing the library and the concentration and particle size of the aerosol to obtain an effect of the aerosol on the absorption spectrum; And (d) after subtracting the data of the absorption spectrum from the influence value, the concentration of the plurality of substances can be measured by comparing the database with a specific algorithm as in the third embodiment. In the above embodiment, the influence value is the absorbance provided by the suspended particles in the absorption spectrum, and by subtracting the influence value, the pure water and the absorbance provided by the plurality of substances can be obtained. In addition, the database as a reference value is one of the contents of the entire database. As in the above embodiment, the database as the second reference value may have the following two functions: (1) obtaining the influence value of the wastewater from the database with respect to the known particle size and concentration of the suspended particles; And (7) using the database to subtract the spectral information of the far-impact value from the absorbance and absorption peak of the complex material to calculate the concentration of the complex substance. The embodiments described above are merely illustrative for convenience of explanation and are not limited to the invention. Therefore, those skilled in the art can revise and change the examples without departing from the spirit of the present invention. However, the scope of the patent application is not limited. [The simple description of the drawings] The first figure (4) is the implementation of the case " Absorption spectrum of 1 〇 at different concentrations; Figure-8 (8) is the relationship between the different concentrations and absorbance of the particle size 1 () handsome (four) floating particles in 201124712; the first figure (c) For the first embodiment of the present invention, the absorbance of visible light with a wavelength of 500 nm at different particle sizes and different concentrations of suspended particles; the second figure (A) is a spectrum of sodium phthalate at different concentrations; Figure 2 (B) is the spectrum of chromium ions (Cr3+) at different concentrations; the third figure (A) is the spectrum of the same particle number of suspended particles at different particle sizes; the third figure (B) is the different particles The relationship between the size and the wavelength of the absorption peak; the fourth diagram is a flow chart of a second embodiment of the present invention; the fifth diagram is a flowchart of a fourth embodiment of the present invention; and the sixth diagram is an implementation of the present invention. The flow chart of Example 5. [Description of main component symbols] Steps S41 to S49 Steps S51 to S57 Steps S61 to S65 19