TW201102005A - A disinfection method for aquatic products cultivation - Google Patents

Abstract

The present invention provide a disinfection method for aquatic products cultivation, including various applications of chlorine dioxide solution. The concentrations of chlorine dioxide for small fish transportation is ranged from 0.005 to 0.01ppm, for adult fish transportation ranged from 0.01 to 0.05ppm, for the pool disinfection ranged 0.01 to 3.00ppm, for the empty pool sterilization ranged from 400 to 500ppm, for water in the fish pool ranged from 0.3 to 2.0ppm, for fish disease prevention ranged from 0.1 to 1.5ppm and adding new chlorine dioxide every 15 days, for fish disease disinfection ranged 0.1 to 2.5ppm and adding new chlorine dioxide solution every other day till the situation improved, for the crab/decapod/ turtle/frog disease prevention ranged from 0.6 to 0.9ppm and adding new chlorine dioxide every 15 days, for the crab/decapod/ turtle/frog disease disinfection ranged from 0.6 to 1.5 ppm and adding new chlorine dioxide every other day till the situation improved, for the baby crab/ decapod disease prevention ranged from 0.05 to 0.20ppm and adding new chlorine dioxide every 3 days, for the baby crab/ decapod disease disinfection ranged from 0.05 to 0.60ppm and adding new chlorine dioxide every other day till the situation improved.

Description

201102005 無 五、本案若有化學式時，請齡最_示㈣特徵的化學式 六、發明說明： 【發明所屬之技術領域】 "本發明屬於水產養殖之消毒、殺菌處理方法之應用相關技201102005 None 5. If there is a chemical formula in this case, please ask for the chemical formula of the characteristics of the age of the fourth. 4. Description of the invention: [Technical field of invention] "This invention belongs to the application of disinfection and sterilization treatment methods for aquaculture

=領域，_是-種朗二氧化氯在水產養殖之環境使用消 毋、殺菌處理方法之應用。 【先前技術】 在以往大規模的水處理系統中，消毒是最基本的處理工 藝，氨和氯的衍生物消毒是經濟有效和應用最廣泛的消毒，然 而隨著對氯消毒產生的副產物的分析研究，發現氣與水中某些 有機物如腐植酸、富裏酸等反應會產生大量的齒代氰和氯化& 機物，使得處理後的水中各類氣化有機物有不同程度的提高， 氣氣和氯的衍生物消毒的副作用產生三鹵甲烷(THMs)等，三 鹵甲烷的前驅物質通常來自三類：一類是天然大分子有機 物，如腐植酸、灰黃霉酸等；另一類是小分子有機物，如酸類 化合物、苯胺、苯、氨基酸等多種有機物；第三類是藻類及代 謝產物。三齒甲烷(THMS)等致癌等之危害程度越來越引起人 們的重視’消毒效果受環境影響，水體中之pH及溫度等皆會 影響消毒效果，對病毒性病原消毒效果較差，除腺病毒外，腸 道病毒之殺菌效果差，長期使用會使微生物產生較強之抗藥 性。積極尋找替代氯的更安全更優越的新型氯化消毒劑，已經 成為水處理的一個發展方向。 201102005 二氧化氯是-種強氧化劑，其氧化能力居於祕、NaC1〇2、 KMn〇4、NaCIO、似必、過氧乙酸等強氧化劑之首，也是一種比 含氯消毒劑更加優秀的消毒劑，其氧化能力是翠氣的2. 6倍。 二氧化氯易溶於水，其溶解度為氣的5倍。與氣不同的是，"二 氧化氣在水中不易發生水解反應’但是在水產養殖業的實務應 用及操作準則及用量，尚未被詳加研究與證實。 【發明内容】 本發明的目的在於提供一種水產養殖之消毒殺菌方法，其 攀117包括使用二氧化氯水溶液。 、 本發明使用一氧化氣水溶液於魚苗運輸槽之消毒較佳範圍 介於0.005〜O.Olppm之間；使用於成魚運輸槽之消毒較佳範圍介 於0.0卜0.05ppm之間；使用於養殖池之消毒較佳範圍介於 〇· 01〜3. OOppm之間；使用於養殖池之空池底池壁消毒較佳範圍介 於400〜500ppm之間；使用於養殖池之空池水體消毒較佳範圍介於 〇. 3〜2. Oppm之間；使用於養殖池之魚類養殖疾病預防較佳範圍介 於〇. 1〜1. 5ppm之間每15天添加一次；使用於養殖池之魚類養殖 # 疾病治療較佳範圍介於〇. 1〜2. 5ppm ppm之間每2天添加一次直到 改善；使用於養殖池之蝦蟹蛙鱉類疾病預防較佳範圍介於 〇· 6〜0. 9ppm ppm之間每15天添加一次；使用於養殖池之蝦蟹蛙 繁類疾病治療較佳範圍介於〇. 6〜1. 5ppm ppm之間每2天添加一次 直到改善；使用於養殖池之蟹類蛋狀幼體蝦類幼體養殖疾病預防 較佳範圍介於〇. 05〜〇. 20ppm ppm之間每3天添加一次；使用於養 殖池之蟹類蛋狀幼體蝦類幼體養殖疾病治療較佳範圍介於 0.05〜0.60ppm ppm之間每2天添加一次直到改善；應用在弧菌之 抑菌作用較佳濃度介於〇. 8〜1. 2xl(T3mol/m3間。 201102005 —因本發明所使用之二氧化氣可改善養殖水體的水質 ，增加水 體，氧的含量，具有良好的水質淨化效果，能觸加水環境中 的冷氧及減低化學耗氧量和減值，減少水體富營養化，提供適 宜的養殖水環境。 ，因本發明之較佳實施例，證明使用本發明之方法，二氧化氣 ^毒效果不受pH相素影響，能避免產生三齒代產生。能分解 丄害物質，如鐵鋪子、硫化物與氰化物，而提供—種殺菌能力 尚，並可以較短時間、較少用量、操作方便、且安全可靠的方式 ，去水中水產養雜系中的細g，以制優良水質之化氣在 水產養殖應用。 為讓本發明之上述和其他目的、雖和優職更明顯易懂， 文特舉較佳實施例，並配合所關式，作詳細說明如下。 【實施方式】 本發明之實施例以二氧化氣實施於水產養殖之不同運輸方式 ^不同養殖體系之使財法與用量，及二氧化氣水產養殖之 ^毒與殺菌使用濃度與使用方式，二氧化氯對水中化學因子之影 響及二氧化氯的殺菌作用之使用。 實施例一：二氧化氣於水產養殖之殺菌力 *本實施例在水產養殖應用，針對溶藻弧菌、溶藻弧菌、溶血 狐菌、乳酸鏈球g、酵母g、以二氧絲做消毒測試，結果如表j 所示： 試驗對象 Vibrio alginolyticus 溶藻弧菌（宿主：白蝦) 接觸8ϋ |使用濃度| 5®in 0. 5ppm 殺死 99. 99%= field, _ is the application of the method of sulphur dioxide and sterilizing treatment in the environment of aquaculture. [Prior Art] In the large-scale water treatment system in the past, disinfection is the most basic treatment process. The disinfection of ammonia and chlorine derivatives is the most cost-effective and widely used disinfection, but with the by-products of chlorine disinfection. Analytical studies have found that the reaction of gas with certain organic substances in water such as humic acid and fulvic acid produces a large amount of cyanide and chlorinated & ampoules, which can improve the various types of vaporized organic matter in the treated water to varying degrees. The side effects of gas and chlorine derivatives disinfection produce trihalomethanes (THMs), etc. The precursors of trihalomethanes usually come from three categories: one is natural macromolecular organics such as humic acid, fulvic acid, etc.; the other is Small molecules of organic matter, such as acid compounds, aniline, benzene, amino acids and other organic matter; the third category is algae and metabolites. The degree of harm caused by carcinogenicity such as tridentate methane (THMS) has attracted more and more people's attention. 'The disinfection effect is affected by the environment. The pH and temperature in the water body will affect the disinfection effect. The disinfection effect on viral pathogens is poor, except for adenovirus. In addition, the enterovirus has a poor bactericidal effect, and long-term use will make the microorganisms more resistant to drugs. Actively looking for a new safer and superior chlorinated disinfectant that replaces chlorine has become a development direction for water treatment. 201102005 Chlorine dioxide is a strong oxidant. Its oxidizing ability is the first of strong oxidants such as secret, NaC1〇2, KMn〇4, NaCIO, ubiquitous, peracetic acid, etc. It is also a better disinfectant than chlorine disinfectant. 2倍。 The oxidizing capacity is 2. 6 times. Chlorine dioxide is readily soluble in water and has a solubility of 5 times that of gas. Unlike gas, "2 Oxidation gas is not susceptible to hydrolysis in water', but the practical application and operating guidelines and dosages in the aquaculture industry have not been studied and confirmed in detail. SUMMARY OF THE INVENTION An object of the present invention is to provide a method for disinfecting and sterilizing aquaculture, which comprises using an aqueous solution of chlorine dioxide. The disinfecting solution of the aqueous solution of the aqueous solution of the present invention is preferably between 0.005 and 0.1 ppm; The preferred range of disinfection is between 〇· 01~3. OOppm; the optimal range of disinfection of the empty pool bottom wall used in the culture pond is between 400 and 500 ppm; the preferred range of disinfection of the empty pond water used in the culture pond is between 〇. 3~2. Oppm; the preferred range of fish culture disease prevention used in culture ponds is between 1. 1~1. 5ppm is added every 15 days; fish culture used in breeding ponds# Between 1 and 2. 5ppm ppm is added every 2 days until improvement; the preferred range of prevention of shrimp and crab frog mites used in the culture pond is between 〇·6~0. 9ppm ppm added every 15 days. Once; the treatment of shrimp and crab frogs used in breeding ponds is better than 6. 6~1. 5ppm ppm is added every 2 days until improvement; crab larvae used in culture ponds The best range of prevention is between 〇. 05 〇. Add every 3 days between 20ppm ppm; the treatment of crab larvae larvae used in culture ponds is preferably between 0.05~0.60ppm ppm and added every 2 days until improvement; applied in arc The preferred concentration of the bacteriostatic action of the bacteria is between 8. 8~1. 2xl (T3mol/m3. 201102005) The oxidizing gas used in the present invention can improve the water quality of the aquaculture water, increase the water content and oxygen content, and has good The water purification effect can touch the cold oxygen in the water environment and reduce the chemical oxygen consumption and the value, reduce the eutrophication of the water body, and provide a suitable aquaculture water environment. According to the preferred embodiment of the present invention, the invention is proved to be used. The method of oxidizing gas is not affected by pH phase, and can avoid the generation of three-tooth generation. It can decompose harmful substances such as iron shops, sulfides and cyanide, and provide a kind of bactericidal ability, and can Shorter time, less dosage, convenient operation, safe and reliable way, the fine g in the aquatic aquaculture system is removed to make the good quality water gas in aquaculture application. To make the above and the present invention The purpose, although better and easier to understand, is described in detail with reference to the preferred embodiment, and is described in detail below. [Embodiment] Embodiments of the present invention implement different transportations of aquaculture using dioxide gas. Mode ^ The use of different farming systems and the amount of use, and the concentration and use of chlorine dioxide aquaculture and the use of chlorine dioxide on the chemical factors in water and the use of chlorine dioxide sterilization. A: The bactericidal power of dioxide gas in aquaculture * This example is used in aquaculture applications, for Vibrio alginolyticus, Vibrio alginolyticus, hemolytic fox fungus, lactic acid chain ball g, yeast g, and disinfection test with dioxane. The results are shown in Table j: Test subject Vibrio alginolyticus Vibrio alginolyticus (host: white shrimp) contact 8 ϋ | use concentration | 5®in 0. 5ppm kill 99. 99%

氧，氣於水產養殖殺菌力測試結果 201102005Oxygen, gas in aquaculture bactericidal test results 201102005

Vibrio parahaemolyticus 溶血弧菌（宿主：九孔） 5min 〇· 5ppm 殺死100% Damsela弧菌（宿主：草瑕） 5min 〇. 5ppin 殺死100% Lactococcus garvieae 乳酸鏈球菌（宿主·•淡水長臂大蝦） 5min 〇· 5ppm 殺死卯.99% Debaryomyces hansenii 酵母菌（宿主：淡水長臂大蝦） 5min 〇. 5ppm 殺死100% 根據本實施例結果顯示，在加入二氧化氣後，其殺菌力皆為 99%以上，顯示二氧化氣應用於水產養殖是具成效的。 實施例二：二氧化氯潑灑後水產養殖中之細菌數量的變化Vibrio parahaemolyticus Vibrio parahaemolyticus (host: nine wells) 5min 〇· 5ppm kills 100% of Vibrio damsela (host: grasshopper) 5min 〇. 5ppin kills 100% Lactococcus garvieae Streptococcus lactis (host · • freshwater long-armed prawns 5min 5· 5ppm Kill 卯.99% Debaryomyces hansenii Yeast (host: freshwater long-armed prawns) 5min 〇. 5ppm Kill 100% According to the results of this example, the bactericidal power after adding the oxidizing gas More than 99%, it shows that the application of dioxide gas to aquaculture is effective. Example 2: Changes in the number of bacteria in aquaculture after chlorine dioxide splashing

本實施例以二氧化氣之不同的濃度與加藥後之時間針對水產 養殖中之細菌變化，進行測試其殺菌、消毒之效果，使用二氧化In this embodiment, the concentration of the dioxide gas and the time after the dosing are applied to the bacteria in the aquaculture, and the sterilization and disinfection effects are tested, and the oxidation is used.

氣濃度為 0· 2 mg/L、0.4 mg/L、0. 6mg/L、0. 8 mg/L、1. 0 mg/L 測試細菌數量變化。並將實驗結果列於表2。The gas concentration was 0. 2 mg/L, 0.4 mg/L, 0.6 mg/L, 0.8 mg/L, and 1.0 mg/L. The experimental results are listed in Table 2.

表2 不同濃度的Cl〇2溶液潑麗後養蝦水泥池水中細菌數量的蠻化 時間 藥物濃度（mg/L) $ ί·細菌數量（cfu/mL) (小時） 0.2 0.4 0.6 0.8 1.0 對照組 1 4. 8x104 4. 8x1 〇3 3. 6x103 4. 2x103 3. 2χ103 7. 6x107 2 6. 5x103 4. 3x1 〇3 4. 2x102 4. 5x103 3. 6x103 7. 4x107 3 6. 2x103 4. 5χ103 3. 7x103 6. ΙχΙΟ3 4. 6x103 7. 2χ107 4 6. 8xl03 5.1χ1〇3 4. 2χ103 5. 3χ103 5. ΙχΙΟ3 7. 9χ107 5 9·8χ103 6_2χ103 6.4χ103 5. ΙχΙΟ3 5. 3x103 8. 4x107 6 5. 8χ104 5.5χ103 5. 5x103 6. 2x103 4. 2x103 7. 8x107 7 6. lxl〇5 5. 7x1 〇3 6. 3x103 6. ΙχΙΟ3 4. 6x103 7. 4x107 8 6. 5x10s 6. 4x105 6.4x105 6. 7x103 7. 3x104 7. 5x107 9 7. 2x106 7. 7x1 〇7 7. 2x105 7. ΙχΙΟ4 7. 3x105 7. 4x107 10 7. 2x1 〇6 7. 5x1 〇6 7.7x106 7.5x105 9. 3x106 7. 8x107 11 8. 7x106 8. 6x106 7. 3x106 7. 8x105 8. 3x106 7. 3x107 12 8. 3x106 7. 8x1 〇7 8. 4x106 7.4x107 7. 8x107 7. 5x107 根據本實施例結果顯示，在潑灑二氧化氣溶液後，各試驗池 的細菌數量均迅速下降，且使用二氧化氣濃度愈高，在潑灑後時 間介於1〜6h内，其飼養池水中細菌數量即大幅的下降，使水體 中的細菌數量保持了較低的水準。 201102005 實施例三：二氧化氱於魚苗運輸之消毒方法 將二氧化氯用清水稀釋成濃度介於〇· 005~0. 01ppm間，將其 均勻喷麗於魚苗運輸槽内，即能達到良好的消毒殺菌效果。 實施例四：二氧化氣於成魚運輸之消毒方法 使用清水稀釋二氧化氯使其濃度介於〇· 0卜〇· 〇3ppm間，在將 其均勻喷灑於成魚運輸槽内，即能達到有效的消毒殺菌能力。 實施例五：二氧化氣於養成管理之消毒方法 鲁 使用二氧化氯濃度介於0. 01〜0· 02ppm間，將其均勻讓於養殖 池内’即能達到良好的消毒殺菌效果。 實施例六：二氧化氣於池底池壁消毒方法 在清除池塘底部污泥後，於注水前一天，利用清水稀釋二氧 化氯使其濃度介於400〜500ppm間，喷灑魚池體及池壁，即能達到 消毒殺菌的效果。 • 實施例七：二氧化氯於空池水艘消毒方法 將一氧化氣以清水稀釋成濃度介於0. 5〜1. 〇ppm間，將其均句 灑於養殖池内’ 3天後無殘留即可直接放養，且需依水質狀況增減 用量。 實施例八：二氧化氣於魚類養殖之浸泡方法 以清水將二氧化氣用稀釋成濃度介於1. (M· 5ppm間，浸泡在 魚類養殖池’時間介於5〜10分鐘，即能達到良好的消毒殺菌效 I ° ,. 201102005 實施例九：二氧化氣於魚類養殖之預防方法 將一氧化氯用清水稀釋成濃度介於0. 8〜1. Oppm間，喷麗於魚 類養殖池，約15天全池潑灑1次，即能達到有效的預防效果。 實施例十：二氧化氣於魚類養殖之治療方法 使用二氧化氯濃度介於0.1〜1. 5ppm間，噴灑魚類養殖池，如 病情嚴重時則需隔日再使用1次，以達到良好的治療效果。 • 實施例十一：二氧化氯於瑕蟹蛙鱉類養殖之預防方法 使用清水將二氧化氯稀釋成濃度介於〇· 6〜0. 8ppm間，喷灑於 蝦蟹蛙鱉類養殖池，約10〜15天全池潑灑1次，即能達到有效的 預防效果。 實施例十二：二氣化氣於瑕蟹蛙鱉類養殖之治療方法 將一氧化亂用清水稀釋成濃度介於1· 0〜1· 5ppm間，噴丨麗於瑕 蟹蛙鱉類養殖池’如病情嚴重時則需隔曰再使用1次，即能这丨 • 有效的治療效果。 實施例十三：二氧化氣於蟹類蛋狀幼體蝦類幼通養殖之預防方法 使用二氧化氣濃度介於0.05〜0.2ppm間，喷灑於蟹類蛋狀幼 體瑕類幼體養殖池，約2〜3天全池潑瀵1次，即能達到有效的預 防效果。 實施例十四：二氧化氣於蟹類蛋狀幼體蝦類幼艘養殖之治療方法 將二氧化氯用清水稀釋成濃度介於〇. 15〜225ppm ^，噴灑 201102005Table 2 Bruising time of bacteria in the cement pool water of different concentrations of Cl〇2 solution. Drug concentration (mg/L) $ ί·Number of bacteria (cfu/mL) (hours) 0.2 0.4 0.6 0.8 1.0 Control group 1 4. 8x104 4. 8x1 〇3 3. 6x103 4. 2x103 3. 2χ103 7. 6x107 2 6. 5x103 4. 3x1 〇3 4. 2x102 4. 5x103 3. 6x103 7. 4x107 3 6. 2x103 4. 5χ103 3 7x103 6. ΙχΙΟ3 4. 6x103 7. 2χ107 4 6. 8xl03 5.1χ1〇3 4. 2χ103 5. 3χ103 5. ΙχΙΟ3 7. 9χ107 5 9·8χ103 6_2χ103 6.4χ103 5. ΙχΙΟ3 5. 3x103 8. 4x107 6 5. 8χ104 5.5χ103 5. 5x103 6. 2x103 4. 2x103 7. 8x107 7 6. lxl〇5 5. 7x1 〇3 6. 3x103 6. ΙχΙΟ3 4. 6x103 7. 4x107 8 6. 5x10s 6. 4x105 6.4x105 6. 7x103 7. 3x104 7. 5x107 9 7. 2x106 7. 7x1 〇7 7. 2x105 7. ΙχΙΟ4 7. 3x105 7. 4x107 10 7. 2x1 〇6 7. 5x1 〇6 7.7x106 7.5x105 9. 3x106 7. 8x107 11 8 7x106 8. 6x106 7. 3x106 7. 8x105 8. 3x106 7. 3x107 12 8. 3x106 7. 8x1 〇7 8. 4x106 7.4x107 7. 8x107 7. 5x107 According to the results of this example, the dioxide gas solution is splashed After each test pool The number of bacteria decreased rapidly, and the higher the concentration of the dioxide used, the time after the splash was between 1 and 6 hours, the number of bacteria in the feeding pool was greatly reduced, and the number of bacteria in the water body was kept at a low level. . 201102005 Example 3: Disinfection method of cerium oxide in fry transportation The chlorine dioxide is diluted with water to a concentration of 〇·005~0. 01ppm, and it is evenly sprayed in the fry transport tank, which can achieve good Sterilization effect. Example 4: Disinfection method of dioxin in adult fish transportation Diluted chlorine dioxide with water to make it have a concentration of 〇·0〇·〇3ppm, and it can be effectively sprayed evenly in the adult fish transport tank. Disinfection ability. Example 5: Disinfection method for the management of the formation of the oxidizing gas. The concentration of the chlorine dioxide is between 0. 01~0· 02ppm, and it is uniformly distributed in the culture tank to achieve a good disinfection effect. Example 6: Disinfection method of dioxide gas in the bottom of the pool wall After removing the sludge at the bottom of the pond, dilute the chlorine dioxide with water to make the concentration between 400~500ppm, spray the fish pond body and the pool wall one day before water injection. , can achieve the effect of sterilization. • Example 7: Chlorine dioxide in an empty pond water disinfection method. The oxidizing gas is diluted with water to a concentration of 0. 5~1. 〇ppm, the average sentence is sprinkled in the culture pond. It can be directly stocked and needs to be increased or decreased depending on the water quality. Example 8: Soaking method of dioxin in fish culture The dilute gas is diluted with water to a concentration of 1. (M· 5ppm, soaked in a fish culture pond) for 5 to 10 minutes, which can be achieved. The sterilizing effect of the sterilizing and sterilizing effect I ° , 201102005 Example 9: The prevention method of the oxidizing gas in the fish culture, the chlorine dioxide is diluted with water to a concentration of 0. 8~1. 5小时之间,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, If the condition is serious, it needs to be used again every other day to achieve a good therapeutic effect. • Example 11: Prevention of Chlorine Dioxide in the Crab Frog Breeding Method Use water to dilute the chlorine dioxide to a concentration of 〇· 6~0. 8ppm, sprayed on the shrimp and crab frog scorpion breeding pool, about 10~15 days, the whole pool can be splashed once, which can achieve effective preventive effect. Example 12: Two gasification 瑕 瑕 crab frog The treatment of ape culture will be oxidized The water is diluted to a concentration between 1·0~1·5ppm, and the squirting squid is used in the frog frog culture pool. If the condition is serious, it needs to be separated and used once more, which is the effective treatment effect. Example 13: Preventive method for the production of sulfur dioxide in crab egg-shaped juvenile shrimps. The concentration of the dioxide gas is between 0.05 and 0.2 ppm, and is sprayed on the larvae of the crab larvae. After 2 to 3 days of whole pond pour 1 time, it can achieve effective preventive effect. Example 14: Treatment method of dioxin in crab egg-shaped juvenile shrimps, young fish culture, dilute chlorine dioxide into water with water Between 15. 15~225ppm ^, spraying 201102005

於類蛋狀幼體蝦類幼體養殖池，如病情嚴重時則需隔日再使用 次’即能達到有效的治療效果。實施例十五：二氧化氣對魚池水中化學因子的影響 本發明以二氧化氣之不同濃度與加藥前加藥後之溶解氧 (D0)、化學耗氧量(C0D)與氨氮值(腿3_幻之最佳使用濃度，二氧化 氣使用濃度為 0 xl(T3mol/m3、0. 2 xl(T3m〇l/m3、〇. 5 xi〇-3m〇i/m3、 〇· 8 xlO—W/nM. 2 xlO—W/m3，且針對二氧化氣之加藥前〇. %， 與加藥後lh、6h、16h、24h進行化學因子的測量，並將實驗結果列表3。 3 表 時間 加藥前0.5h 加 二氧化氣對魚池水化，In the egg-like juvenile shrimp larvae culture pond, if the condition is serious, it needs to be used again every other day to achieve an effective therapeutic effect. Example 15: Effect of Dioxide on Chemical Factors in Fish Pond Water According to the present invention, dissolved oxygen (D0), chemical oxygen demand (C0D) and ammonia nitrogen value (legs) after different concentrations of dioxide gas and before dosing The optimal use concentration of 3_ magical, the concentration of sulfur dioxide used is 0 xl (T3mol/m3, 0. 2 xl (T3m〇l/m3, 〇. 5 xi〇-3m〇i/m3, 〇·8 xlO- W/nM. 2 xlO—W/m3, and the chemical factors were measured before and after the dosing of the dioxide gas. The chemical factors were measured at lh, 6h, 16h, and 24h after dosing, and the experimental results were listed in 3. 3 Adding oxidizing gas to the fish pond for hydration 0.5 h before the time of dosing,

因子的影響 PHFactor effect PH

9 201102005 Ο^ΙΙΖΓΙΙΓΟίϊΕΕΙΓ'Πΐη 本實施例證實： ~ U一- 1. /合解氧(J)0).使用二氧化氯2後，即能明顯地提升養殖水環境中 的/合解氧含里。而使用二氧化氣濃度為lxl0_3mol/心後，養魚 池水中的最大增氧量為104%。 2·化子耗氧$(_)與氨氮值⑽3_N):使用二氧化氯後，即能有 效地降低養殖水環境+的化學耗氧量⑽^和·_N含量，其降 低百分，與二氧化驗度有關，故養魚池使用二氧化氣濃度為 1.2xl(T3m〇l/m3後’化學耗氧量(c〇D)下降的最大百分率為39 8 • %，NHs-N清除率以33. 3%為最佳。 實施例十六··二氧化氣對水中細菌總數及孤菌數的影響 本實施例以二氧化氣之不随度與加藥後之時間，對水中細 菌^數及孤g數的影響’測試其殺g能力，使用二氧化氯濃度為〇 xio mol/y、0. 2 xl(T3m〇i/m3、〇 5 xl0-3m〇1/m3、〇 8 xl0-3m〇1/m3、 1·2 xl〇3mol/m3，測其細菌數之變化，並將實驗結果列於下表4。 表49 201102005 Ο^ΙΙΖΓΙΙΓΟίϊΕΕΙΓ'Πΐη This example shows: ~ U-- 1. / Oxygen (J) 0). After using chlorine dioxide 2, it can significantly improve the oxygen content in the culture water environment. in. When the concentration of the dioxide gas was lxl0_3mol/heart, the maximum oxygen uptake in the fish pond water was 104%. 2. Oxygen consumption of oxygen (() and ammonia nitrogen value (10) 3_N): After using chlorine dioxide, it can effectively reduce the chemical oxygen demand (10) ^ and · _N content of the culture water environment, which is reduced by The oxidation test is related. Therefore, the concentration of CO2 in the fish pond is 1.2xl (the maximum percentage of chemical oxygen demand (c〇D) decreases after T3m〇l/m3 is 39 8 • %, and the NHs-N clearance rate is 33. 3% is the best. Example 16···························································································· The influence of g number 'test its killing ability, using chlorine dioxide concentration 〇xio mol/y, 0. 2 xl (T3m〇i/m3, 〇5 xl0-3m〇1/m3, 〇8 xl0-3m〇 1/m3, 1·2 xl〇3mol/m3, the change of the bacterial number was measured, and the experimental results are listed in the following Table 4. Table 4

加藥後時間 --藥物濃度(xl0_3mo】 /in3) 0.0 0.2 0.5 0.8 1.2 總 0. 5 + + + + + + + + + + + + + + + 菌 16. 0 + + + + + + + + + + + + 数 48. 0 + + + + + + + + + + + + 弧 0.5 + + + + + + + + + + + + + + + 菌 16. 0 + + + + - - 數 48.0 + + + + + + 使用一氧化氣濃度在1.2xi〇-3m〇i/m3時’即能有效地抑制海 水中的細菌總數，在加入二氧化氯後的16h，即有明顯抑菌作用， 其藥力在48h後相對減弱’而藥物濃度介於on 2xi〇-3m〇i/m3, 對弧菌有較佳的抑菌作用。 201102005Time after dosing - drug concentration (xl0_3mo) /in3) 0.0 0.2 0.5 0.8 1.2 Total 0. 5 + + + + + + + + + + + + + + + + bacteria 16. 0 + + + + + + + + + + + + number 48. 0 + + + + + + + + + + + + arc 0.5 + + + + + + + + + + + + + + + + bacteria 16. 0 + + + + - - number 48.0 + + + + + + When the concentration of monoxide is 1.2xi〇-3m〇i/m3, it can effectively inhibit the total number of bacteria in seawater. After 16 hours of adding chlorine dioxide, it has obvious antibacterial effect. The drug strength is relatively weak after 48h' and the drug concentration is between 2xi〇-3m〇i/m3, which has a better antibacterial effect on Vibrio. 201102005

加人二氧化氯之溶魄⑽、娜耗氧量㈣)、NM 解氧氯之㈣於水產養財，測試其溶 驗結果列於/以觸與氨氮值⑽3~ν)之含量變化，並將實 圖1顯示加藥後的6h内各組溶解氧 後平穩下降’其中以二氧化氣濃度3. _ = 升^勢= 持較高的溶氧值。 ，在51小時維Adding chlorine dioxide solution (10), Na oxygen consumption (4), NM oxychloride (4) in aquaculture, testing the results of the test listed / in contact with ammonia nitrogen (10) 3 ~ ν) content changes, and Figure 1 shows that the dissolved oxygen in each group is steadily decreased within 6 hours after dosing. The concentration of the gas is 3. _ = liter potential = higher dissolved oxygen value. In 51 hours dimension

人二3顯示，加人二氧化氯後化學耗氧量(C 與Γ襲，隨著加藥後時間延長，化學耗氧量(⑽、 ΝΗ3-Ν含里逐漸下降，在時間介於16〜24h降到最低點。 雖然本發明已以較佳實施例揭露如上，然其並非用以限定 本發明，任何熟習此技藝者，在不麟本發明之精神和範圍 内’當可作些許之更動與潤飾，因此本發明之保護範圍當視後 附之申請專利範圍所界定者為準。 【圖式簡單說明】 • 圖1為添加不同濃度之二氧化氯後，其溶解氧(D0)值隨時間之 變化。 圖2為加入不同濃度之二氧化氯後，其化學耗氧量(c〇D)值隨 時間之變化。 圖3為添加不同濃度之二氧化氯後，其NHs-N值隨時間之變化。 【主要元件符號說明】Human 2 shows that the chemical oxygen demand after adding chlorine dioxide (C and attack, with the prolonged time after dosing, the chemical oxygen demand ((10), ΝΗ3-Ν) gradually decreased, at a time between 16~ 24h is reduced to the lowest point. Although the present invention has been disclosed in the above preferred embodiments, it is not intended to limit the present invention, and any person skilled in the art can make some changes in the spirit and scope of the present invention. And the scope of protection of the present invention is defined by the scope of the appended patent application. [Simplified description of the drawings] • Figure 1 shows the dissolved oxygen (D0) value of different concentrations of chlorine dioxide added. The change of time. Figure 2 shows the change of chemical oxygen demand (c〇D) value with time after adding different concentrations of chlorine dioxide. Figure 3 shows the NHs-N value with different concentrations of chlorine dioxide. Change of time. [Main component symbol description]

Claims (1)

201102005 七、申δ青專利範圍： 一種水產養殖的消毒殺菌方法，其中包括使用二氧化氯水 溶液。 2、 ，據申請專利範圍第1項所述之一種水產養殖的消毒殺 菌作業’其中二氡化氣使用於魚苗運輸槽之消毒較佳範圍 介於0· 005〜〇· 〇ippm之間。 3、 ，據申請專利範圍第1項所述之一種水產養殖的消毒殺 菌作業’其中二氧化氣使用於成魚運輸槽之消毒較佳範圍201102005 VII. Scope of patent application: A method of disinfection and sterilization of aquaculture, including the use of chlorine dioxide water solution. 2. A disinfection and bactericidal operation for aquaculture according to item 1 of the scope of application of the patent application. The disinfection of the sputum gas used in the fry transport tank is preferably between 0·005~〇·〇ippm. 3. A disinfection and bactericidal operation of aquaculture according to item 1 of the scope of application of the patent application, wherein the oxidizing gas is used in the sterilization range of the adult fish transport tank. 5、 6、5, 6, 8、 介於0. 01〜〇. 〇5ppm之間。 ，據申請專利範圍第1項所述之一種水產養殖的消毒殺 菌作業’其中二氧化氣使用於養殖池之消毒較佳範圍介於 〇· 01 〜3. 00ppm 之間。 根據申請專利範圍第丨項所述之一種水產養殖的消毒殺 菌作業’其中二氧化氣使用於養殖池之空池底池壁消毒較 佳範圍介於400〜50〇ppm之間。 根據申請專利範圍第1項所述之一種水產養殖的消毒殺 菌作業’其中二氧化氯使用於養殖池之空池水體消毒較佳 範圍介於0.3〜2. 〇ppm之間。 2艮據申叫專利範圍第1項所述之一種水產養殖的消毒殺 菌作業，其中一氡化氣使用於養殖池之魚類養殖疾病預防 較佳範圍介於Q. 1〜1. 5ppm之間每15天添加-次。 根據申請專利範圍第1項所述之-種水產養殖的消毒殺 菌作業，其中二氧化氣使用於養殖池之魚類養殖疾病治療 較佳範圍介於〜2. 5ppm ppm之間每2天添加-次直到改 9、 根據申請專利範圍第1項所述之-種水產養殖的消毒殺 12 201102005 菌作業，其中二氧化氯使用於養殖池之蝦蟹蛙鱉類疾病預 防較佳範圍介於〇· 6〜〇· 9ppm ppm之間每丨5天添加一次。 W、根據申請專利範圍第丨項所述之一種水產養殖的消毒殺 鹵作業，其中一氧化氯使用於養殖池之蝦蟹娃繁類疾病治 療較佳範圍介於〇. 6〜1. 5ppm ppm之間每2天添加一次直到改 善。8, between 0. 01~〇. 〇5ppm. According to the scope of the patent application, the disinfection and bactericidal operation of aquaculture is as follows: wherein the disinfection of the oxidizing gas used in the culture pond is preferably in the range of 〇· 01 to 3.00 ppm. According to the above-mentioned patent application scope, a disinfection and bactericidal operation of aquaculture, wherein the oxidizing gas used for the empty pool bottom wall of the culture pond is preferably in the range of 400 to 50 〇 ppm. According to the disinfection and bactericidal operation of aquaculture according to the first aspect of the patent application, the disinfection of the empty water in the aquaculture tank in which the chlorine dioxide is used is preferably between 0.3 and 2. 〇ppm. 2之间之间。 According to the application of the scope of the patent scope of the first paragraph of a sterilizing operation of the aquaculture, wherein the use of a phlegm gas in the culture of the fish culture disease prevention range is better than Q. 1~1. 5ppm between every 15 Days added - times. According to the scope of the patent application, the aquaculture sterilization operation described in the first paragraph of the patent application, wherein the treatment of the fish culture disease in which the dioxide gas is used in the culture pond is preferably between -2. 5 ppm ppm and added every two days until Change 9, in accordance with the scope of the application for patents, the aquaculture sterilization 12 201102005 bacteria operation, in which the chlorine dioxide used in the culture pond shrimp crab frog disease prevention range is better than 〇 · 6 ~ 〇 · Add once every 5 days between 9ppm ppm. W 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 It is added every 2 days until improvement. 11、根據中請專利範㈣i項所述之—種水產#殖的消毒殺 菌作業’其中二氧化氣使用於養殖池之蟹類蛋狀幼體瑕類 幼體養殖疾病預防較佳範圍介於〇. 05〜〇· 2〇ppm ppm之間每3 天添加一次。 12 13 根據申請專利範圍第1項所述之—種水產養殖的消毒殺 菌作業，其中二氧化氣使驗養殖池之_蛋狀幼體瑕類 幼體養殖疾病治療較佳範圍介於〇· 05〜〇. 6〇ppm ppm之間每2 天添加一次直到改善。 根據申請專利範圍第1項所述之-種水產養殖的消毒殺 菌方法，其中二氧化氯應用在弧菌之抑菌作 於 0.8〜1.2xl(T3m〇l/m3 間。 /辰度”11. According to the patent application (4) item i of the patent, the disinfection and sterilization operation of a kind of aquaculture# is used for the prevention of the disease of the larvae of the larvae of the larvae of the larvae of the larvae used in the culture pond. 〇· 2〇ppm ppm is added every 3 days. 12 13 According to the scope of the patent application, the disinfection and sterilization operation of aquaculture, in which the dioxide gas makes the treatment of the larvae of the larvae of the larvae of the brooding pond is better than 〇· 05~〇. Add every 2 days between 6 〇 ppm ppm until improvement. According to the disinfection and bactericidal method for aquaculture according to the scope of claim 1, wherein the chlorine dioxide is applied to the antibacterial activity of Vibrio at 0.8~1.2xl (T3m〇l/m3. /辰度) 1313