201015035 九、發明說明： 【發明所屬之技術領域】 本發明是有關於一種冷凍裝置與冷凍方法，特別是指 一種生物組織之冷凍包埋裝置與冷凍包埋方法。 【先前技術】 在許多臨床醫學與生物學研究領域中，經常需要透過 生物組織冷凍切片來觀察判斷生物組織之生理狀離。生物 . 組織冷凍切片製作前，必需先將欲進行冷凍切片之生物会且 • 織以包埋劑包覆後，再透過冷凍降溫之程序，達到進行切 片時可提供所需支撐硬度的目的。雖然多數要進行冷床切 片之生物組織在進行冷凍包埋前，皆會經過抗凍處理，以 避免生物組織中之水分形成冰晶，而破壞該生物組織形態 完整性。即使生物組織已經進行抗凍處理，但如冷凍包埋 過程的降溫速度較慢，致使冷凍時間過長時，亦無法完全 防止冰晶之形成，且會增加相關操作之時間與成本。 傳統生物組織之冷凍包埋方法主要分兩大類，其中一 粵冑是以低'皿冰箱或冷柬庫來進行冷;束固化，但此種方式受 限於6又備所能提供之冷卻降溫速度較慢’經常需超過十分 - 鐘才能達到冷凍包埋的目的❶ —另種方法是以超低溫液化氣體（如液態氮，_196〇c ) '降包埋，該方法是將欲包埋之生物組織置入添加冷 東包埋劑之磁开+ ^'中’然後將該棋型置於液態氮中 >待模型 _ ^ ^ /、包埋劑冷凍固化後，再將固化成型之生物組織 脫模取出，而6士4 &战包埋之程序。雖然此種方法之降溫速度 5 201015035 較快，但操作時須具備儲存液態氮之設備，且超低溫液化 氣體之操作亦需有適當之防護設備與人員訓練，安全性需 求與成本皆較高。另外，冷凍包埋後之生物組織要進行切 片時’還得再等生物組織冷凍塊（一 i 9 6。〇 )回溫至與切片 機設定溫度大約一致時（約_3(rc )才能進行切片作業實 際上並無法真正縮短時間。 此外，上述兩種方法於完成冷凍包埋後，由於所使用 的模具大部分皆為塑膠材質，在脫模過程中，多採破壞式 脫模而無法重複使用，以致生物組織的包埋成本提高。 【發明内容】 $ 因此，本發明之目的，即在提供一種冷凍包埋速度較 快之冷凍包埋裝置。 本發明之另一目的，在於提供一種可快速脫模而不會 破壞模具構件的冷;東包埋裝置。 本發明之再一目的，在於提供一種可快速冷凍包埋生 物組織的冷凍包埋方法。 於是，本發明生物組織之冷;東包埋裝置，豸用於將生 =組織與包埋生物組織之包埋劑冷涞定型該冷涞裝置包 機口及女裝於機台上之冷卻機構，該冷卻機構包 可調變溫度的冷卻單元’及一可拆離地安裝於冷卻單-几上之中空的模座，該冷卻單元具有一朝上之低溫冷卻面， 而該模座具有一低溫環壁部，且該低溫冷卻面與該低溫 環壁部相配合界定出一開口朝上並可容置生物組織與包埋 劑’且使生物組織與包埋劑冷核型之成型空間。 201015035 於是，本發明卩上述生物組織之冷來包埋農置之冷束 包埋方法，包含以下步驟（a)將生物組織與用以包埋生物 組織之包埋劑置入該成型空間中而與該低溫冷卻面及低溫 環壁β接觸’及（b)啟動冷卻單元，使該低溫冷卻面溫度 降低至零下-預定溫度值，使包埋劑於成型空間中固化成 型而包埋固定該生物組織。 【實施方式】 有關本發明之前述及其他技術内容、特點與功效’在 • 以下配合參考圖式之一個較佳實施例的詳細說明中，將可 清楚的呈現。 如圓1〜3所示，本發明生物組織之冷凍包埋裝置的較 佳實施例，適用於快速冷凍包埋生物組織1〇(^該冷凍包埋 裝置包含一機台3，及分別安裝於機台3上之一冷卻機構4 、一脫模機構5與一監控機構6。 該冷卻機構4包括一安裝於機台3上之冷卻單元41、 一可拆離地安裝於冷卻單元41上之中空模座42,及一可拆 〇 離地插置於模座42中之塞塊43。該冷卻單元41包括一安 裝於機台3中之散熱器411、一安裝於散熱器411之一頂面 * 4121之電熱致冷器414、一疊接於電熱致冷器414頂面並 具有一朝上之低溫冷卻面419的導熱板418，及一覆蓋包覆 該電熱致冷器414與導熱板418而僅使該低溫冷卻面419 外露地固設於散熱器411上之隔熱塊41〇。 該散熱器411為水冷式散熱器，具有一界定出該頂面 4121之中空水冷座412，及一與水冷座412連通並可持續 201015035 將低溫冷卻水輸送通過水冷座412而散除水冷座412之熱 能的循環散熱模組413 ^該電熱致冷器414是由二上下疊接 於該水冷座412頂面4121之電熱致冷晶片415構成，並具 有一與導熱板418接觸之朝上低溫面416,及一抵接於水冷 座412頂面4121之朝下高溫面417。由於該散熱器411與 電熱致冷晶器414皆為習知構件，因此不再詳述。 該模座42具有一可脫離地設置於隔熱塊41〇頂面之座 本體421 ’及一嵌裝固定於座本體421上並疊置於該導熱板 418之低溫冷卻面419的環狀導熱環423。該座本體421具 有一供導熱環423往上嵌裝固定之直立插孔422,該導熱環 423是抵接於該低溫冷卻面419，，並界定出一自該低溫冷 卻面419往上延伸之環狀低溫環壁面424，且該低溫環壁面 424與該低溫冷卻面419相配合界定出一開口朝上之成型空 間40 〇 該塞塊43具有一可脫離地插置於插孔422中之塊本體 431，及一固定於塊本體431底面並往下限位靠抵於該導熱 環423頂緣而蓋封該成型空間40開口的導熱片432。 在本實施例中，導熱板418、導熱環423與導熱片432 皆為熱良導體，都是由銅製成，而隔熱塊410、座本艘421 與塊本體431皆為冷熱絕緣艎’是由鐵氟龍製成，但實施 時’該導熱板418、導熱環423與導熱片432材質，及該隔 熱塊410、座本體421與塊本體43ί材質皆不以此為限。 如囷1、4、5所示’該脫模機構5包括一安裝固定於 機台3頂面之定位座51、一可拆離地插置於定位座51中之 201015035 • 承接座53，及一固設於機台3上且位於定位座51上方之脫 模單元52。該定位座51具有一 0J設於其頂面且延伸至其前 側面之取料槽511、一凹設於其頂面且延伸至前側面並與取 料槽51〗連通之定位槽512，及一凹設於其前側面並與該取 料槽511連通並可供插承接座53嵌插地位之插槽513，且 該定位槽512之左右寬度大於取料槽51〇，深度小於取料槽 51〇，可供該模座42嵌置定位，而使該模座42之插孔422 與取料槽511連通。 參 °亥脫模單元52包括一固定於定位座51上方之固定座 521、一可被驅動而往下突伸入定位槽512地插置於固定座 521之頂推桿522、一固定於頂推桿522底端面且由冷熱絕 緣原料製成之抵推塊523，及一安裝於固定座mi中並可被 驅動而驅使頂推桿522往下突伸之驅動模組524，且該驅動 模組524具有一外露於固定座521右側並可被扳.動樞擺， 而傳動該頂推桿522帶動抵推塊523上下移動之扳動桿525 〇 • 該承接座53頂面凹設有二前、後間隔且分別與取料槽 411連通之承接槽530,且其中一承接槽53〇是位於該頂推 桿522正下方。 該監控機構6包括一嵌置外露於機台3上之並可顯示 導熱板418溫度的顯示器61，及一安裝於機台3並用以驅 動控制該電熱致冷器414與散熱器411之作動的中控器62 ，且該中控器62可感測該導熱板418之溫度；並具有—外 露於機台3前側面且可被扳動而控制電熱致冷器：414之作 201015035 動的控溫開關621，該控溫開關621可兩段式調控該電熱致 冷器414,其中一段為預冷功能，另外—段為冷凍功能，當 預冷功能被啟動時，中控器62會驅使電熱致冷器414調降 其低溫面之溫度，直至熱傳導接觸之該導熱板418溫度降 至〇°c ’當冷凍功能被啟動時，電熱致冷器414會調降其低 溫面416溫度，直至導熱板418溫度下降至零下一預定溫 度，在本實施例中，該導熱板418之冷凍溫度為H，但 實施時不以此為限。由於該中控器62可感測導熱板418溫 度與控制電熱致冷器414降溫皆為習知技術，因此不再詳 述。 ^ 如圖1、3所示，該冷凍包埋裝置用以冷凍包埋生物組 織方法包含以下步驟： 步驟（一）置入生物組織100並封閉成型空間4〇。將 預定冷凍包埋之生物組織100置入成型空間40中，並將用 以包埋生物組織100之包埋劑101填滿成型空間40，然後 將該塞塊43***插孔422中，使該導熱片432限位靠抵於 該導熱環42；3頂緣，而蓋封成型空間4〇。 & 步驟（二）冷凍成型。扳動該控溫開關621而啟動冷 凍功能，使低溫冷卻面419、導熱環423與導熱片432同步 降溫至-3(TC，使得整個成型空間4〇處於·3(Γ(：低溫狀態， 進而快速將包埋劑101與生物組織1〇〇的熱能吸收散除，-而快速地將生物組織1〇〇與包埋劑1〇1冷凍成型。在本實 施例中，該電熱致冷器414可在三分鐘内將成型空間4〇内 之溫度降至-3(TC，而使生物組織100與包埋劑1〇1迅逮冷 10 201015035 • 凍成型。 , 於上述冷凍過程中，該散熱器411會持續將昶對低溫之 冷卻水打入水冷座412中，迅速散除電熱致冷器414之高 溫面417的熱能，降低該高溫面417溫度，使查熱致冷器 414維持在一穩定之效能，相對使得該低溫面416可快速地 降低至預定之冷;東溫度。於此過程中’該顯示器61會同步 顯示該導熱板418溫度。 如圖3〜5所示，步驟（三）脫模。待生物組織1〇〇與 0 包埋劑101冷凍形成冷凍塊體200後，將該模座42拆離冷 卻單元41 ’此時，冷凍成型之冷凍塊體2〇〇會固結於模座 42之低溫環壁面424，接著，將該模座42嵌置定位於定位 座51之定位槽512中，並扳動該驅動模組524.之扳動桿 525，驅使該頂推桿522連動抵推塊523往下突伸***模座 42之插孔422中，將冷凍塊體200往下頂推脫離模座42， 而掉落至取料槽5 11中，便完成生物組織j〇〇之冷凍包埋作 業。由於此時該冷凍塊體200之溫度大致相等於切片機（ ® 圖未示）之溫度，因此可直接以切片機進行生物組織切片 作業’不需再等待該冷凍塊體200回溫，有助於縮短生物 . 組織100包埋與切片之作業時間。 在本實施例中，於該模座42上插置該塞塊43的目的 ，疋要該冷熱絕緣性隔熱塊410、座本體421與塊本體431 完全隔絕外來熱源的影響，使整個冷凍空間4〇完全處於低 溫狀態，但實施時，該塞塊43並非必要，僅藉击該導熱板 418與導熱環423之低溫，即可快速完成生物組織1〇〇之冷 11 201015035 束包埋作業。此外，固設於該頂推桿522底端面之抵推塊 523是由冷熱絕緣材料製成的結構設計，可於脫模過程中， 避免冷束塊體200直接接觸相對高溫物體或熱良導體而吸 熱融化，但實施時，不以設置該抵推塊523為必要。 另外，在完成步驟（二）之冷凍成型，並將模座42拆 離冷卻單元41後，可扳動該控溫開關621而啟動預冷功能 ，使該低溫冷卻面419回溫至0。(：，連帶使導熱環423的低 溫環壁面424同步回溫至（TC，進而使成型空間4〇維持在 〇°C狀態，可方便使用者準備進行下一次之冷凍包埋作業，脅 可節省自室溫冷卻至0°C的時間。 歸納上述，透過該冷卻單元41之導熱板418、模座42 之導熱環423的低溫環壁面424，及該塞塊Μ之導熱片 432的熱傳導性接觸結構設計，除了有助於迅速降低成型空 間40溫度外，還可使包埋劑1〇1與生物組織1〇〇直接接觸 該等導熱板418、低溫環壁面424與導熱片432，進而可快 速冷凍包埋生物組織100，再加上該模座42與該脫模機構 5之結構設計，可在完全不破壞模座42的情況下，完整地◎ 取出固結成型於模座42中之生物組織冷凍塊體2〇〇，使該 模座42可重複使用，而可降低冷凍包埋之耗材成本。且可 藉由該電熱致冷器414之低溫面416溫度設計，使冷凍成 型後之冷凍塊體200溫度大致相等於切片機溫度，使該冷 凍塊體200可直接進行切片作業，有助於大幅縮短生物組 織包埋切片作業所需花費的時間，且沒有使用低溫液態氣 體之危險性，相當方便實用，且安全性高。因此，確實可 12 201015035 * 達到本發明之目的。 淮以上所述者，僅為本發明之一較佳實施例而已，當 不能以此限定本發明實施之範圍，即大凡依本發明申請專 利範圍及發明說明内容所作之簡單的等效變化與修佛，皆 仍屬本發明專利涵蓋之範圍内。 【圖式簡單說明】 圖1疋本發明生物組織之冷凍包埋裝置的較佳實施例 之立體圖； φ 圖2是該較佳實施例的-冷卻機構的立體分解圖； 圖3是冷卻機構之前側視組合剖視圖； 圖4是該較佳實施例之前側視剖面圖，說明一模座置 放定位於一定位座時的情況；及 圖5是類似圖4之視圖，說明一冷來成型之冷束塊體 被脫模單元往下頂離模座時的情況。 13 201015035 【主要元件符號說明】 100..... ...生物組織 424·.·. ....低溫環壁面 101…·. ...包埋劑 ， 43 .·.·· ....塞塊 200··..· ...冷凉·塊體 431.... ....塊本體 3........ ...機台 432·.·. ....導熱片 4........ ...冷卻機構 5....... ....脫模機構 40 ....... ...成型空間 51 ..·· ....定位座 41 ...... ...冷卻單元 511.... ....取料槽 411 ····· ...散熱器 512.... ....定位槽 412.·... ...水冷座 513.... —插槽 4121 ... ,頂面 52 ..... .…脫模單元 413·..· ...循環散熱模組 521.... ….固定座 414·.··· ...電熱致冷器 522.... ....頂推桿 415 ....電熱致冷晶片 523.... ....抵推塊 416·.··. ,低溫面 524.... ....驅動模組 417..... ,1%溫面 525.... ....扳動桿 418..... ....導熱板 53 .…· ....承接座 419···.. ....低溫冷卻面 530...· ....承接槽 410·.·· ....隔熱塊 6....... ....監控機構 42 ...... ....模座 61 ..··· ....顯示器 421·... ----座本體 62 ···.· ....中控器 422···· ....插孔 621.... ....控溫開關 423··.· ....導熱環201015035 IX. Description of the Invention: [Technical Field] The present invention relates to a freezing apparatus and a freezing method, and more particularly to a biological tissue freezing embedding apparatus and a freezing embedding method. [Prior Art] In many fields of clinical medicine and biological research, it is often necessary to observe the physiological traits of biological tissues by cryosectioning of biological tissues. Biology. Before the tissue cryosection is prepared, it is necessary to first freeze the biofilm to be frozen and then wrap it with an embedding agent, and then pass through the freezing and cooling process to achieve the desired support hardness when cutting. Although most biological tissues subjected to cold bed cutting are subjected to freeze-frozen treatment before freezing and embedding, the formation of ice crystals in the biological tissue is prevented, and the biological integrity of the biological tissue is destroyed. Even if the biological tissue has been subjected to antifreeze treatment, if the temperature of the freezing process is slow, the freezing time is too long, and the formation of ice crystals cannot be completely prevented, and the time and cost of the related operation are increased. The traditional methods of freezing and embedding of biological tissues are mainly divided into two categories. One of them is cold in a low-refrigerator or cold-camp library; beam curing, but this method is limited by the cooling and cooling provided by 6 The slower speed often takes more than ten minutes to reach the purpose of freezing and embedding. Another method is to embed the ultra-low temperature liquefied gas (such as liquid nitrogen, _196〇c), which is the organism to be buried. The tissue is placed in the magnetic opening + ^ 'medium of the cold east embedding agent and then the chess type is placed in the liquid nitrogen > after the model _ ^ ^ /, the embedding agent is freeze-cured, and then the solidified biological tissue is solidified. Release the mold, and the 6 士 4 & Although the cooling rate of this method is faster than 201015035, it must be equipped with equipment for storing liquid nitrogen. The operation of ultra-low temperature liquefied gas also requires appropriate protective equipment and personnel training, and the safety requirements and costs are high. In addition, when the frozen tissue is to be sliced, it is necessary to wait for the biological tissue freezing block to return to the temperature of the slicer (about _3 (rc)). In fact, the above two methods can not really shorten the time after the completion of the freeze embedding. Since most of the molds used are made of plastic materials, in the process of demolding, the demolition mold release is repeated and cannot be repeated. Use, so that the embedding cost of the biological tissue is increased. SUMMARY OF THE INVENTION Accordingly, it is an object of the present invention to provide a cryoemulsion device having a relatively fast freezing embedding speed. Another object of the present invention is to provide a Quickly demoulding without damaging the cold of the mold member; East embedding device. A further object of the present invention is to provide a cryoemulsion method capable of rapidly freezing and embedding biological tissue. Thus, the biological tissue of the present invention is cold; The embedding device is used for cold-setting the embedding agent of the raw tissue and the embedded biological tissue to fix the cooling mechanism of the cold-pressing device and the cooling mechanism of the women's wear on the machine table, the cooling mechanism a temperature-changing cooling unit' and a hollow mold base detachably mounted on the cooling unit, the cooling unit having an upwardly-lowering cooling surface, and the mold base having a low-temperature annular wall portion And the low temperature cooling surface cooperates with the low temperature ring wall portion to define a molding space with an opening facing upward and can accommodate the biological tissue and the embedding agent 'and cold tissue type of the biological tissue and the embedding agent. 201015035 Thus, the present invention The cold-embedding method for embedding the agricultural tissue in the cold tissue comprises the following steps: (a) placing the biological tissue and the embedding agent for embedding the biological tissue into the molding space and the cryogenic cooling surface And the low temperature ring wall β contact 'and (b) start the cooling unit to lower the temperature of the low temperature cooling surface to a sub-zero predetermined temperature value, so that the embedding agent is solidified in the molding space to embed and fix the biological tissue. The foregoing and other technical contents, features, and advantages of the present invention will be apparent from the following detailed description of a preferred embodiment of the accompanying drawings. FIG. The preferred embodiment of the frozen embedding device of the object tissue is suitable for rapidly freezing the embedded biological tissue 1 (the freezing embedding device comprises a machine table 3, and a cooling mechanism 4 respectively mounted on the machine table 3, A demolding mechanism 5 and a monitoring mechanism 6. The cooling mechanism 4 includes a cooling unit 41 mounted on the machine base 3, a hollow mold base 42 detachably mounted on the cooling unit 41, and a detachable cymbal. The plug block 43 is inserted into the die holder 42. The cooling unit 41 includes a heat sink 411 mounted in the machine base 3, and an electric heat cooler 414 mounted on a top surface of the heat sink 411*41. a heat conducting plate 418 stacked on the top surface of the electrothermal cooler 414 and having an upwardly facing low temperature cooling surface 419, and a cover coating the electrothermal refrigerator 414 and the heat conducting plate 418 to expose only the low temperature cooling surface 419 The heat insulating block 41 is fixed to the heat sink 411. The heat sink 411 is a water-cooled heat sink having a hollow water-cooling seat 412 defining the top surface 4121, and a water-cooling seat 412 communicating with the water-cooling seat 412 and continuing to transport the low-temperature cooling water through the water-cooling seat 412 to disperse the water-cooled seat 412. The thermal heat-dissipating module 413 of the thermal energy is composed of two electrothermal cooling fins 415 which are superposed on the top surface 4121 of the water-cooling base 412, and has an upward-low temperature surface which is in contact with the heat conducting plate 418. 416, and a downwardly facing high temperature surface 417 abutting the top surface 4121 of the water cooling seat 412. Since the heat sink 411 and the electrothermal crystallizer 414 are both conventional members, they will not be described in detail. The die holder 42 has a seat body 421 ' detachably disposed on the top surface of the heat insulating block 41 and an annular heat conduction embedded on the seat body 421 and superposed on the low temperature cooling surface 419 of the heat conducting plate 418. Ring 423. The base body 421 has an upright insertion hole 422 for mounting the heat-conducting ring 423 upwardly. The heat-conducting ring 423 abuts against the low-temperature cooling surface 419 and defines an upward extending from the low-temperature cooling surface 419. An annular low temperature annular wall surface 424, and the low temperature annular wall surface 424 cooperates with the low temperature cooling surface 419 to define an opening-facing molding space 40. The plug block 43 has a block detachably inserted into the socket 422. The main body 431 is fixed to the bottom surface of the block body 431 and abuts against the top edge of the heat conducting ring 423 to seal the heat conducting sheet 432 of the forming space 40. In this embodiment, the heat conducting plate 418, the heat conducting ring 423 and the heat conducting sheet 432 are both good heat conductors, both of which are made of copper, and the heat insulating block 410, the seat boat 421 and the block body 431 are both hot and cold insulating. It is made of Teflon, but the material of the heat conducting plate 418, the heat conducting ring 423 and the heat conducting sheet 432, and the materials of the heat insulating block 410, the seat body 421 and the block body 43 are not limited thereto. As shown in 囷 1, 4, 5, the demolding mechanism 5 includes a positioning base 51 mounted on the top surface of the machine table 3, a 201015035 detachably inserted into the positioning base 51, and a receiving seat 53, and A demolding unit 52 is fixed on the machine table 3 and located above the positioning base 51. The positioning seat 51 has a receiving groove 511 which is disposed on the top surface thereof and extends to the front side thereof, a positioning groove 512 which is recessed on the top surface thereof and extends to the front side and communicates with the take-up groove 51, and a slot 513 recessed in the front side thereof and communicating with the take-up slot 511 for inserting the socket 53 into the socket, and the left and right width of the positioning slot 512 is larger than the take-up slot 51〇, and the depth is smaller than the take-up slot 51〇, the mold base 42 can be positioned and positioned, and the socket 422 of the mold base 42 is connected to the take-out groove 511. The ejector unit 52 includes a fixing base 521 fixed to the positioning base 51, a top push rod 522 that can be driven to protrude downward into the positioning slot 512 and inserted into the fixing seat 521, and is fixed to the top. a pushing block 523 formed by a bottom end surface of the push rod 522 and made of a cold and heat insulating material, and a driving module 524 mounted in the fixing base mi and driven to drive the pushing rod 522 to protrude downward, and the driving module The 524 has a triggering rod 525 which is exposed on the right side of the fixing base 521 and can be pivoted and pivoted, and the pushing rod 522 is driven to move up and down the pushing block 523. The top surface of the receiving seat 53 is recessed. And a receiving groove 530 which is spaced apart from each other and communicates with the take-out groove 411, and one of the receiving grooves 53 is located directly below the pushing rod 522. The monitoring mechanism 6 includes a display 61 embedded on the machine table 3 and capable of displaying the temperature of the heat conducting plate 418, and a display unit 3 mounted on the machine table 3 for driving and controlling the operation of the electric heat cooler 414 and the heat sink 411. The central controller 62, and the central controller 62 can sense the temperature of the heat conducting plate 418; and has - exposed to the front side of the machine table 3 and can be pulled to control the electric heat cooler: 414 for the 201015035 dynamic control The temperature switch 621, the temperature control switch 621 can regulate the electric heat cooler 414 in two stages, one of which is a pre-cooling function, and the other section is a freezing function. When the pre-cooling function is activated, the central controller 62 drives the electric heating. The chiller 414 lowers the temperature of the low temperature surface until the temperature of the heat conducting plate 418 in contact with the heat conduction drops to 〇 °c 'When the freezing function is activated, the electric heat cooler 414 lowers the temperature of the low temperature surface 416 until the heat conduction The temperature of the plate 418 is lowered to a predetermined temperature. In this embodiment, the temperature of the heat transfer plate 418 is H, but is not limited thereto. Since the central controller 62 can sense the temperature of the heat conducting plate 418 and control the temperature drop of the electric heating cooler 414, it is a conventional technique and will not be described in detail. As shown in Figures 1 and 3, the method for freezing the embedded biological tissue of the cryoemulsion device comprises the following steps: Step (1) Inserting the biological tissue 100 and closing the molding space. The predetermined frozen embedded biological tissue 100 is placed in the molding space 40, and the embedding agent 101 for embedding the biological tissue 100 is filled into the molding space 40, and then the plug 43 is inserted into the insertion hole 422, so that the The heat conducting sheet 432 is positioned to abut against the top edge of the heat conducting ring 42; 3, and the sealing space 4 is sealed. & Step (2) Freeze molding. The temperature control switch 621 is pulled to activate the freezing function, so that the low temperature cooling surface 419, the heat conducting ring 423 and the heat conducting sheet 432 are simultaneously cooled down to -3 (TC, so that the entire molding space 4 〇 is at -3 (Γ (: low temperature state, and further The thermal energy absorption of the embedding agent 101 and the biological tissue is quickly dissipated, and the biological tissue 1〇〇 and the embedding agent 1〇1 are rapidly formed into a frozen shape. In the present embodiment, the electrothermal refrigerator 414 is used. The temperature in the molding space can be reduced to -3 (TC) within three minutes, and the biological tissue 100 and the embedding agent 1〇1 can be quickly cooled. 10 201015035 • Frozen molding. In the above freezing process, the heat dissipation The heater 411 continuously pushes the low temperature cooling water into the water cooling seat 412, rapidly dissipates the heat energy of the high temperature surface 417 of the electric heating cooler 414, lowers the temperature of the high temperature surface 417, and maintains the heat detecting cooler 414 at one The stable performance is such that the low temperature surface 416 can be quickly lowered to a predetermined cold; the east temperature. In the process, the display 61 synchronously displays the temperature of the heat conducting plate 418. As shown in Figures 3 to 5, the steps (three ) demoulding. The biological tissue 1〇〇 and 0 embedding agent 101 are frozen to form a cold After the frozen block 200 is removed, the die holder 42 is detached from the cooling unit 41 ′. At this time, the frozen formed frozen block 2 〇〇 is fixed to the low temperature ring wall surface 424 of the die holder 42 , and then the die holder 42 is embedded. The positioning rod 512 is positioned in the positioning slot 51 of the positioning block 51, and the triggering rod 525 of the driving module 524 is pulled to drive the pushing rod 522 to extend downwardly into the socket 422 of the clamping block 523. The freezing block 200 is pushed down from the die holder 42 and dropped into the take-up groove 5 11 to complete the freezing and embedding operation of the biological tissue. Since the temperature of the frozen block 200 at this time It is roughly equivalent to the temperature of the microtome (not shown), so it can be used for bio-tissue slicing directly with the microtome. 'There is no need to wait for the frozen block 200 to warm up, which helps to shorten the organism. 100-embedded and sliced tissue In the present embodiment, the plug block 43 is inserted into the die holder 42 for the purpose of completely isolating the influence of the external heat source from the cold heat insulating block 410, the seat body 421 and the block body 431. Keeping the entire freezing space 4 〇 completely at a low temperature, but when implemented, the plug 43 is not Therefore, only by the low temperature of the heat conducting plate 418 and the heat conducting ring 423, the cold tissue of the biological tissue can be quickly completed. In addition, the pressing block is fixed on the bottom end surface of the pushing rod 522. 523 is a structural design made of cold and heat insulating material, which can prevent the cold bundle block 200 from directly contacting the relatively high temperature object or the good heat conductor to absorb heat during the demolding process, but in implementation, the resisting block 523 is not provided. In addition, after the cold forming of the step (2) is completed, and the mold base 42 is detached from the cooling unit 41, the temperature control switch 621 can be pulled to start the pre-cooling function, and the low-temperature cooling surface 419 is warmed up to 0. (:, the low temperature ring wall surface 424 of the heat conducting ring 423 is synchronously brought back to the temperature (TC, thereby maintaining the molding space 4〇 in the 〇 ° C state, which is convenient for the user to prepare for the next freezing embedding operation, and the threat can be saved. The time from the room temperature to 0 ° C. In summary, the heat conducting plate 418 of the cooling unit 41, the low temperature ring wall surface 424 of the heat conducting ring 423 of the die holder 42, and the thermally conductive contact structure of the heat conducting sheet 432 of the plug block The design, in addition to helping to rapidly reduce the temperature of the molding space 40, can also directly contact the embedding agent 1〇1 and the biological tissue 1〇〇 with the heat conducting plate 418, the low temperature ring wall surface 424 and the heat conducting sheet 432, thereby allowing rapid freezing. The biological tissue 100 is embedded, and the structural design of the mold base 42 and the demolding mechanism 5 can completely remove the biological tissue consolidated in the mold base 42 without destroying the mold base 42 at all. The frozen block 2〇〇 makes the mold base 42 reusable, and the cost of the frozen embedded consumable can be reduced. The frozen block can be frozen by the temperature design of the low temperature surface 416 of the electrothermal cooler 414. Body 200 temperature is approximately equal to The temperature of the slicer allows the frozen block 200 to be directly sliced, which helps to greatly shorten the time required for the biological tissue to be embedded and sliced, and does not have the risk of using a low-temperature liquid gas, which is quite convenient and practical, and is safe. Therefore, it is true that 12 201015035 * achieves the object of the present invention. The above is only a preferred embodiment of the present invention, and the scope of the present invention cannot be limited thereto, that is, the application according to the present invention The simple equivalent changes and the modifications made by the patent scope and the description of the invention are still within the scope of the patent of the present invention. [Fig. 1] FIG. 1 is a preferred embodiment of the frozen embedding device of the biological tissue of the present invention. 2 is a perspective exploded view of the cooling mechanism of the preferred embodiment; FIG. 3 is a front cross-sectional view of the cooling mechanism; FIG. 4 is a front cross-sectional view of the preferred embodiment, illustrating a mode The case where the seat is placed in a positioning seat; and FIG. 5 is a view similar to FIG. 4, illustrating that a cold formed cold bridging block is pushed down from the die holder by the demolding unit. Situation. 13 201015035 [Explanation of main component symbols] 100........... Biological tissue 424···. .... low temperature ring wall surface 101...·.. embedding agent, 43 .···· .... Plug 200··..·...cold and cool block 431...... Block body 3..............machine 432·.. .... Thermal sheet 4........ Cooling mechanism 5........ Demoulding mechanism 40....... molding space 51. ........ Positioning seat 41............Cooling unit 511.....Reclaiming trough 411 ·····...heat sink 512.... ...positioning slot 412.·... water-cooled seat 513.... - slot 4121 ..., top surface 52 ..... .... demoulding unit 413 ·..... Circulating heat dissipation module 521.....fixing seat 414·····Electrical heat cooler 522.....Pushing rod 415 ....Electrical heat-cooling wafer 523.. .. .... against push block 416·.., low temperature surface 524..... drive module 417....., 1% warm surface 525.... Pulling rod 418...... heat conducting plate 53 ..... .... receiving seat 419···.....low temperature cooling surface 530...·.... receiving groove 410 ···· ....Insulation block 6..............Monitoring mechanism 42........ Mold base 61 ..··· .... 421·... ---- Seat body 62 ·····....Central controller 422····.. Jack 621..... Temperature control switch 423 ····....thermal ring
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