SU839547A1 - Method of obtaining leukocytic interferon - Google Patents

Method of obtaining leukocytic interferon Download PDF

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Publication number
SU839547A1
SU839547A1 SU792750539A SU2750539A SU839547A1 SU 839547 A1 SU839547 A1 SU 839547A1 SU 792750539 A SU792750539 A SU 792750539A SU 2750539 A SU2750539 A SU 2750539A SU 839547 A1 SU839547 A1 SU 839547A1
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USSR - Soviet Union
Prior art keywords
interferon
obtaining
expand
virus
incubated
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SU792750539A
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Russian (ru)
Inventor
Валентин Дмитриевич Соловьев
Всеволод Иванович Огарков
Виктор Исаакович Марченко
Лариса Александровна Монастырева
Александр Васильевич Киктенко
Владимир Викторович Парфенов
Ирина Ивановна Бухарова
Нина Константиновна Преображенская
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Всесоюзный Научно-Исследовательскийинститут Биологического Приборострое-Ния
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Priority to SU792750539A priority Critical patent/SU839547A1/en
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Publication of SU839547A1 publication Critical patent/SU839547A1/en

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Description

(54) СПОСОБ ПОЛУЧЕНИЯ ЛЕЙКОЦИТАРНОГО ИНТЕРФЕРОНА(54) METHOD FOR OBTAINING LEUKOCITARNY INTERFERON

с т евиной лейкоцитарный интерферон в количестве 100 ед. на 1 мл суспензии. Смесь инкубируют 2ч при затем центрифугируют дл  отделени  клеток от среды, клеки вновь суспензируют в свежей среде 199 с 10%-ной АМЖ, после чего в суспензию внос т вирус-ин .:дуктор. Титр ВБН определ ют предварительно в культуре куриных фибробластов .with t evinous leukocyte interferon in the amount of 100 units. on 1 ml of suspension. The mixture is incubated for 2 hours, then centrifuged to separate the cells from the medium, the glue is re-suspended in fresh medium 199 with 10% AMJ, after which the virus-in. Suspension is added to the suspension. VBN titer is pre-determined in a chicken fibroblast culture.

на I И этапе Определ ют антивирусную активность. Двухкратные разведени  интерфероносодержаадей жидкости внос т в пробирки с трехдневной культурой диплоидных.клеток человека и через 1&-20 ч инкубации при в те же пробирки внос т вирус-индикатор (вирус везикул рного стоматика ВВС, штамм Индиана ) в дозе 100 unflggHa пробирку. Через 48 ч инкубировани  при ,in stage I and stage Antiviral activity is determined. Twofold dilutions of the interferon-containing liquid are introduced into test tubes with a three-day culture of human diploid cells and after 1 & 20 h of incubation, an indicator virus (the air force vesicular dental virus, Indiana strain) is introduced in the same test tube at a dose of 100 unflggHa tube. After 48 h of incubation at

при условии наступлени  полной дегенерации клеток в контрольных пробирках с культурой ткани, не обработанной интерференом, но инфицированной вирусом, определ ют последнее разведение интерферона, защитившее клетки от вирусной деструкции . Это разведение принимают за титр интерферона и выражают его в единицах ме хдународного стандарта Б 69/19.subject to the onset of complete cell degeneration in control tubes with a tissue culture that was not treated with an interference, but infected with a virus, the last dilution of interferon was determined, which protected the cells from viral destruction. This dilution is taken as an interferon titer and expressed in units of international standard B 69/19.

Средний титр свиного лейкоцитарного интерферона в культуре диплоидных клеток человека 400М.ед/ в том случае, если интерферон получают без применени  прайминг-метода , а при использовании его 10000 М.ед/мл. The average titer of porcine leukocyte interferon in human diploid cell culture is 400M. Units / if interferon is obtained without the priming method, and when using it is 10,000 M. units / ml.

В таблице представлена сравнителна , характеристика свиного и человеческого лейкоцитарного интерфероНОВ .The table shows the comparative characteristics of swine and human leukocyte interferons.

1333+4201333 + 420

Claims (1)

Свиньи Предлагаемый способ позвол ет п чить интерферон, активный у челове ка, повысить качество интерферона за счет снижени  содержани  балластных белков и расширить сьарьевую базу. Формула изобретени  Способ получени  лейкоцитарного интерферона I путем заршхени  лей8 ,1±0,58 4835+814 0,70+0,12 крови вирусом-индуктором с Доследующей инкубацией и вьщелёниSM целевого продукта, отличающийс  тем, что, с целью расширени  сырьевой базы, дл  заражени  используют лейкоциты крови свиней и инкубируют в среде, содержащей амниотическую жидкость. Источники информации, прин тые во внимание при экспертизе 1. Авторское свидетельство СССР № 297296, кл. С 12 К 5/00, 1970.Pigs The proposed method allows the production of interferon, which is active in humans, to improve the quality of interferon by reducing the content of ballast proteins and expand the source base. The invention of the method of obtaining leukocyte interferon I by zeshenia leu8, 1 ± 0.58 4835 + 814 0.70 + 0.12 blood by an inducer virus with a further next incubation and an increase of the target product, characterized in that, in order to expand the raw material base, contamination using porcine leukocytes and incubated in medium containing amniotic fluid. Sources of information taken into account during the examination 1. USSR Author's Certificate No. 297296, cl. From 12 to 5/00, 1970.
SU792750539A 1979-04-06 1979-04-06 Method of obtaining leukocytic interferon SU839547A1 (en)

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Application Number Priority Date Filing Date Title
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1983001899A1 (en) * 1981-12-01 1983-06-09 BÉLADI, Ilona Process for the production of human gamma-interferon

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1983001899A1 (en) * 1981-12-01 1983-06-09 BÉLADI, Ilona Process for the production of human gamma-interferon

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