SU1681266A1 - Method for determining the binding capacity of albumin in the blood serum - Google Patents
Method for determining the binding capacity of albumin in the blood serum Download PDFInfo
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- SU1681266A1 SU1681266A1 SU894720987A SU4720987A SU1681266A1 SU 1681266 A1 SU1681266 A1 SU 1681266A1 SU 894720987 A SU894720987 A SU 894720987A SU 4720987 A SU4720987 A SU 4720987A SU 1681266 A1 SU1681266 A1 SU 1681266A1
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- USSR - Soviet Union
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- serum
- albumin
- blood serum
- binding capacity
- diluted
- Prior art date
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Изобретение относитс к медицине. Цель изобретени - упрощение и ускорение способа. Сущность его заключаетс в том, что флуоресцентным способом измер етс св зывание с альбумином флуоресцирующих N-карбоксифенилимидов 4-замещен- ной 1,8-нафталиндикарбоновой кислоты. Дл проведени анализа достаточно 0,01 мл сыворотки крови. Расход красител 11 мг на 1000 анализов. Длительность определени не более 3,5 мин. 3 таблThis invention relates to medicine. The purpose of the invention is to simplify and speed up the method. The essence of it is that fluorescence of 4-substituted 1,8-naphthalene dicarboxylic acid fluorescent N-carboxyphenyl imides is measured by fluorescence. For analysis, 0.01 ml of serum is sufficient. The dye consumption is 11 mg per 1000 analyzes. Duration of determination is no more than 3.5 minutes. 3 tabl
Description
4545
Таблица 1Table 1
Fi и Fa -интенсивности флуоресценции ФС в сыворотках крови, разведенных в X раз, с содержанием альбумина соответственно 51 и 34 г/л.Fi and Fa-fluorescence intensity of PS in blood sera, divorced X times, with albumin content of 51 and 34 g / l, respectively.
) Среднее ± ошибка среднего) Mean ± mean error
Стадии процессаProcess steps
По предлагаемому способуAccording to the proposed method
1.Разведение сыворотки крови1. Blood serum production
2.Добавление ФС2. Add FS
3.Перемешивание3.Mixing
4.Измерение интенсивности флуоресце относительно эталона4. Measurement of fluorescence intensity relative to reference
5.Оценка отклонени от нормы Всего:5. Evaluation of deviations from the norm. Total:
По способу-прототипуAccording to the method prototype
1.Приготовление рабочего раствора крал 1. Preparation of Kral working solution
2.Добавление раствора красител к сывке2. Add a dye solution to the sylvka
3.Инкубирование при 37°С3. Incubation at 37 ° C
4.Добавление насыщенного раствора ристого натри 4. Add saturated sodium hydroxide solution.
5.Перемешивание5. Mixing
6.Инкубирование при 37°С6. Incubation at 37 ° C
7.Перемешивание7. Mixing
8.Фильтрование8. Filtration
9.Разведение9.Production
10.Измерение оптической плотности10. Measurement of optical density
11.Оценка отклонени от нормы Всего:11. Evaluation of deviations from the norm. Total:
Таблица 2table 2
Таблица 3Table 3
Длительность, минDuration, min
0,5 0,3 0,20.5 0.3 0.2
2,0 0,5 3,52.0 0.5 3.5
0,50.5
0,5 30,00.5 30.0
0,30.3
0,2 1440,00.2 1440.0
0.20.2
5.С5.C
0,30.3
1.01.0
0,5 1478.50.5 1478.5
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU894720987A SU1681266A1 (en) | 1989-07-25 | 1989-07-25 | Method for determining the binding capacity of albumin in the blood serum |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU894720987A SU1681266A1 (en) | 1989-07-25 | 1989-07-25 | Method for determining the binding capacity of albumin in the blood serum |
Publications (1)
Publication Number | Publication Date |
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SU1681266A1 true SU1681266A1 (en) | 1991-09-30 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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SU894720987A SU1681266A1 (en) | 1989-07-25 | 1989-07-25 | Method for determining the binding capacity of albumin in the blood serum |
Country Status (1)
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SU (1) | SU1681266A1 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0728745A1 (en) * | 1995-02-21 | 1996-08-28 | Bayer Ag | 1,8-Naphthalimid derivates, process for their preparation and their use as intermediates |
WO2001053829A1 (en) * | 2000-01-18 | 2001-07-26 | Council For The Central Laboratory Of The Research Councils | Lipoprotein assay |
RU2618447C2 (en) * | 2015-08-20 | 2017-05-03 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Астраханский государственный медицинский университет" Министерства здравоохранения Российской Федерации (ФГБОУ ВО Астраханский ГМУ Минздрава России) | Method of assessing severity of intoxication |
-
1989
- 1989-07-25 SU SU894720987A patent/SU1681266A1/en active
Non-Patent Citations (1)
Title |
---|
Чегер С.И. Транспортна функци сывороточного альбумина, 1975, с 183 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0728745A1 (en) * | 1995-02-21 | 1996-08-28 | Bayer Ag | 1,8-Naphthalimid derivates, process for their preparation and their use as intermediates |
WO2001053829A1 (en) * | 2000-01-18 | 2001-07-26 | Council For The Central Laboratory Of The Research Councils | Lipoprotein assay |
US7211441B2 (en) | 2000-01-18 | 2007-05-01 | Council For The Central Laboratory Of The Research Councils | Lipoprotein assay |
RU2618447C2 (en) * | 2015-08-20 | 2017-05-03 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Астраханский государственный медицинский университет" Министерства здравоохранения Российской Федерации (ФГБОУ ВО Астраханский ГМУ Минздрава России) | Method of assessing severity of intoxication |
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