SMT201600147B - Metodi per migliorare l'espressione di proteine ricombinanti - Google Patents

Metodi per migliorare l'espressione di proteine ricombinanti

Info

Publication number
SMT201600147B
SMT201600147B SM201600147T SM201600147T SMT201600147B SM T201600147 B SMT201600147 B SM T201600147B SM 201600147 T SM201600147 T SM 201600147T SM 201600147 T SM201600147 T SM 201600147T SM T201600147 B SMT201600147 B SM T201600147B
Authority
SM
San Marino
Prior art keywords
expression
improve
methods
recombinant proteins
recombinant
Prior art date
Application number
SM201600147T
Other languages
English (en)
Inventor
Howard Robert Grahame Clarke
Original Assignee
Cmc Icos Biolog Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cmc Icos Biolog Inc filed Critical Cmc Icos Biolog Inc
Publication of SMT201600147B publication Critical patent/SMT201600147B/it

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
    • C12N9/003—Dihydrofolate reductase [DHFR] (1.5.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y105/00—Oxidoreductases acting on the CH-NH group of donors (1.5)
    • C12Y105/01—Oxidoreductases acting on the CH-NH group of donors (1.5) with NAD+ or NADP+ as acceptor (1.5.1)
    • C12Y105/01003—Dihydrofolate reductase (1.5.1.3)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
SM201600147T 2009-08-06 2016-05-27 Metodi per migliorare l'espressione di proteine ricombinanti SMT201600147B (it)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23190609P 2009-08-06 2009-08-06
PCT/US2010/044693 WO2011017606A1 (en) 2009-08-06 2010-08-06 Methods for improving recombinant protein expression

Publications (1)

Publication Number Publication Date
SMT201600147B true SMT201600147B (it) 2016-07-01

Family

ID=43544683

Family Applications (1)

Application Number Title Priority Date Filing Date
SM201600147T SMT201600147B (it) 2009-08-06 2016-05-27 Metodi per migliorare l'espressione di proteine ricombinanti

Country Status (15)

Country Link
US (3) US9212367B2 (it)
EP (1) EP2462237B1 (it)
KR (1) KR101800904B1 (it)
CN (1) CN102648285A (it)
AU (1) AU2010279294B2 (it)
CA (1) CA2771410C (it)
DK (1) DK2462237T3 (it)
ES (1) ES2563073T3 (it)
HR (1) HRP20160162T1 (it)
HU (1) HUE028655T2 (it)
NZ (1) NZ598275A (it)
PL (1) PL2462237T3 (it)
SI (1) SI2462237T1 (it)
SM (1) SMT201600147B (it)
WO (1) WO2011017606A1 (it)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK201070194A (en) * 2010-05-08 2011-11-09 Univ Koebenhavn A method of stabilizing mRNA
CN105209625B (zh) * 2013-03-12 2019-03-29 Cmc依科斯生技制品公司 使用杂合chef1启动子的改善重组蛋白表达
KR101446054B1 (ko) * 2013-03-14 2014-10-01 전남대학교산학협력단 재조합 단백질의 과발현을 위한 번역속도 조절용 램프 태그 및 이용
CN107794275B (zh) * 2017-11-06 2020-04-21 河北省微生物研究所 一种产(+)γ-内酰胺酶的重组毕赤酵母及其构建方法和应用
US20210062217A1 (en) 2018-01-10 2021-03-04 Agc Biologics, Inc. Bidirectional chef1 vectors
KR20200082618A (ko) 2018-12-31 2020-07-08 주식회사 폴루스 인슐린 과발현용 램프 태그 및 이를 이용한 인슐린의 제조방법
CN111434693B (zh) * 2019-01-11 2022-03-08 江苏芝大生物科技有限公司 一种抗氧化融合蛋白及应用
CN109979539B (zh) * 2019-04-10 2020-10-02 电子科技大学 基因序列优化方法、装置及数据处理终端
CN112301047A (zh) * 2020-11-02 2021-02-02 江苏东玄基因科技有限公司 一种精确调控重组蛋白表达的方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4704362A (en) 1977-11-08 1987-11-03 Genentech, Inc. Recombinant cloning vehicle microbial polypeptide expression
DE669986T1 (de) * 1992-11-13 1996-10-10 Idec Pharma Corp Völlig unfunktionelle konsensus-kozak-sequenzen zur säugetier-exprimierung.
US5795737A (en) 1994-09-19 1998-08-18 The General Hospital Corporation High level expression of proteins
US5888809A (en) 1997-05-01 1999-03-30 Icos Corporation Hamster EF-1α transcriptional regulatory DNA
AU2003249208B2 (en) * 2002-07-16 2010-03-04 Vgx Pharmaceuticals, Llc Codon optimized synthetic plasmids
CA2498776A1 (en) * 2002-09-13 2004-03-25 The University Of Queensland Gene expression system based on codon translation efficiency
US7344886B2 (en) * 2002-11-29 2008-03-18 Boehringer Ingelheim Pharma Gmbh & Co., Kg Neomycin-phosphotransferase-genes and methods for the selection of recombinant cells producing high levels of a desired gene product
CA2514941A1 (en) * 2003-02-03 2004-08-19 Immuno Japan Inc. High-expression vector for animal cells
EP2808384B1 (en) * 2004-10-08 2017-12-06 The Government of the United States of America as represented by the Secretary of the Department of Health and Human Services Modulation of replicative fitness by using less frequently used synonymous codons
ES2384391T3 (es) * 2004-11-08 2012-07-04 Chromagenics B.V. Selección de células hospedantes que expresan proteína a altos niveles
WO2006063292A1 (en) * 2004-12-08 2006-06-15 Icos Corporation Recombinant method for making multimeric proteins
US7429480B2 (en) * 2005-01-10 2008-09-30 National Taiwan University Promoter sequences from WSSV immediate early genes and their uses in recombinant DNA techniques
WO2007130650A2 (en) * 2006-05-04 2007-11-15 The Regents Of The University Of California Methods for calculating codon pair-based translational kinetics values, and methods for generating polypeptide-encoding nucleotide sequences from such values

Also Published As

Publication number Publication date
CN102648285A (zh) 2012-08-22
KR20120041245A (ko) 2012-04-30
CA2771410C (en) 2020-10-06
EP2462237A1 (en) 2012-06-13
US10066231B2 (en) 2018-09-04
HRP20160162T1 (hr) 2016-04-08
CA2771410A1 (en) 2011-02-10
AU2010279294A1 (en) 2012-03-15
US9212367B2 (en) 2015-12-15
NZ598275A (en) 2014-11-28
EP2462237B1 (en) 2015-12-23
KR101800904B1 (ko) 2017-12-20
US20190225972A1 (en) 2019-07-25
WO2011017606A1 (en) 2011-02-10
ES2563073T3 (es) 2016-03-10
US20120214203A1 (en) 2012-08-23
SI2462237T1 (sl) 2016-04-29
HUE028655T2 (en) 2016-12-28
EP2462237A4 (en) 2013-07-03
PL2462237T3 (pl) 2016-06-30
US20160076043A1 (en) 2016-03-17
AU2010279294B2 (en) 2014-12-04
DK2462237T3 (da) 2016-03-29

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