SK281100B6 - Aspergillus niger ccm 8210 microorganism - Google Patents

Aspergillus niger ccm 8210 microorganism Download PDF

Info

Publication number
SK281100B6
SK281100B6 SK156-96A SK15696A SK281100B6 SK 281100 B6 SK281100 B6 SK 281100B6 SK 15696 A SK15696 A SK 15696A SK 281100 B6 SK281100 B6 SK 281100B6
Authority
SK
Slovakia
Prior art keywords
aspergillus niger
microorganism
ccm
niger ccm
citric acid
Prior art date
Application number
SK156-96A
Other languages
Slovak (sk)
Other versions
SK15696A3 (en
Inventor
Peter Michalík
Original Assignee
Likospol, A. S.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Likospol, A. S. filed Critical Likospol, A. S.
Priority to SK156-96A priority Critical patent/SK281100B6/en
Priority to PCT/SK1996/000014 priority patent/WO1997010350A1/en
Priority to AU70061/96A priority patent/AU7006196A/en
Publication of SK15696A3 publication Critical patent/SK15696A3/en
Publication of SK281100B6 publication Critical patent/SK281100B6/en

Links

Landscapes

  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Aspergillus niger CCM 8210 microorganism is usable for the preparation of citric acid of high purity. The productivity provides a yield of 90 - 93 kg of citric acid per 100 kg of sugar.

Description

Príklady uskutočnenia vynálezuDETAILED DESCRIPTION OF THE INVENTION

Príklad 1Example 1

Pripraví sa hydrolyzát čistého kukuričného škrobu s dextrózovým ekvivalentom DE = 15 %, z ktorého sa pripraví kultivačné médium. 1 liter média obsahuje 180 g cukru, 2,8 g síranu amónneho, 0,3 g dihydrogenfosforečnanu draselného, 0,15 g chloridu vápenatého a 0,08 g heptahydrátu síranu zinočnatého. Sterilné kultivačné médium s pH 2,7 až 2,8 sa v 1001 fermentore zaočkuje s 2 až 3 % obj. 40 až 48 hodinového inokula kmeňa Aspergillus niger CCM 8210 a kultivuje sa pri teplote 32 až 33 °C, obvodovej rýchlosti miešadla do 5.2 m.s1 a vzdušnení 0,2 až 0,35 VVM počas 155 až 160 hodín. V priebehu fermentácie sa vytvorí v médiu 165 g.ľ1 kyseliny citrónovej.A pure corn starch hydrolyzate with a dextrose equivalent of DE = 15% is prepared from which a culture medium is prepared. 1 liter of medium contains 180 g of sugar, 2.8 g of ammonium sulfate, 0.3 g of potassium dihydrogen phosphate, 0.15 g of calcium chloride and 0.08 g of zinc sulfate heptahydrate. A sterile culture medium of pH 2.7 to 2.8 is inoculated with 2 to 3% v / v in a 100 L fermenter. 40 to 48 hour inoculum of Aspergillus niger strain CCM 8210 and cultured at 32-33 ° C, stirrer peripheral speed up to 5.2 ms 1 and aeration 0.2-0.35 VVM for 155-160 hours. 165 g / l of citric acid are formed in the medium during the fermentation.

Príklad 2Example 2

Pripraví sa hydrolyzát kukuričnej múky s dextrózovým ekvivalentom DE = 40 %, z ktorého sa pripraví kultivačné médium. 1 liter média obsahuje 180 g cukru, 2,0 g dusična-Corn flour hydrolyzate is prepared with a dextrose equivalent of DE = 40% from which the culture medium is prepared. 1 liter of media contains 180 g of sugar, 2.0 g of nitrogen-

Claims (1)

PATENTOVÉ NÁROKYPATENT CLAIMS Mikroorganizmus Aspergillus niger CCM 8210 produkujúci vysoké množstvá kyseliny citrónovej bez tvorby vedľajších organických kyselín.Aspergillus niger CCM 8210 producing high levels of citric acid without the formation of organic acids.
SK156-96A 1995-09-11 1996-02-02 Aspergillus niger ccm 8210 microorganism SK281100B6 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
SK156-96A SK281100B6 (en) 1996-02-02 1996-02-02 Aspergillus niger ccm 8210 microorganism
PCT/SK1996/000014 WO1997010350A1 (en) 1995-09-11 1996-09-10 Process, apparatus and microorganism strain for the manufacture of citric acid
AU70061/96A AU7006196A (en) 1995-09-11 1996-09-10 Process, apparatus and microorganism strain for the manufacture of citric acid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SK156-96A SK281100B6 (en) 1996-02-02 1996-02-02 Aspergillus niger ccm 8210 microorganism

Publications (2)

Publication Number Publication Date
SK15696A3 SK15696A3 (en) 1997-09-10
SK281100B6 true SK281100B6 (en) 2000-12-11

Family

ID=20433244

Family Applications (1)

Application Number Title Priority Date Filing Date
SK156-96A SK281100B6 (en) 1995-09-11 1996-02-02 Aspergillus niger ccm 8210 microorganism

Country Status (1)

Country Link
SK (1) SK281100B6 (en)

Also Published As

Publication number Publication date
SK15696A3 (en) 1997-09-10

Similar Documents

Publication Publication Date Title
JPS6155948B2 (en)
CA2210319A1 (en) Production of seasoning
NZ504345A (en) Low pH lactic acid fermentation
JP3717535B2 (en) Novel microorganism and process for producing L-α-amino acid
EP0887420A3 (en) Process for producing L-amino acids by fermentation
US4774179A (en) Process for preparing a 7-aminocephalosporanic acid compound
Tsugawa et al. Production of l-Glutamic Acid from dl-Hydantoin-5-propionic Acid by Microoganisms: Part I. Screening of l-Glutamic Acid-Producing Microorganisms and Some Optimal Conditions for Production of l-Glutamic Acid
US5166061A (en) Process for the production of 4-halo-3-hydroxybutyronitrile with Corynebacterium or Microbacterium
US5210031A (en) Process for the production of R(-)-4-halo-3-hydroxybutyronitrile
JP3951008B2 (en) Capsaicin decomposing / synthesizing enzyme and production method thereof
Choi et al. Hydrolysis of the nitrile group in α-aminophenylacetonitrile by nitrilase; Development of a new biotechnology for stereospecific production of S-α-phenylglycine
CA2042425C (en) Biocatalytic process for the production of l-(-)-carnitine from crotonobetaine and strains of proteeae for use in said process
US3825473A (en) Production of cephalosporin c
US4248967A (en) Enzymic complexes adapted to convert racemic hydantoins into optically active aminoacids, and their applications
JPH07106150B2 (en) Novel L-aminoacylase
SK281100B6 (en) Aspergillus niger ccm 8210 microorganism
US3980520A (en) Process for the production of l-malic acid by microbiological fermentation and means suitable for carrying out the same
JP4300289B2 (en) Hydrolysis or dehydration condensing enzyme, method for producing the enzyme, and method for synthesizing amide using the enzyme
US3239428A (en) Preparation of 6-aminopenicillanic acid by arthrobacter viscosus
CA1140878A (en) Preparation of 2,5-diketogluconic acid
US4734368A (en) Process for the bioconversion of fumarate to L-malate
US6197560B1 (en) Metabolic controlled fermentation procedure for the manufacture of lovastatin hydroxy acid
CA1209073A (en) Process for the biotechnical production of l-malic acid
CA2289553C (en) Metabolic controlled fermentation procedure for the manufacture of lovastatin hydroxy acid
RU2215036C2 (en) Method for preparing citric acid