SG147349A1 - Reaction system - Google Patents

Reaction system

Info

Publication number
SG147349A1
SG147349A1 SG200703238-6A SG2007032386A SG147349A1 SG 147349 A1 SG147349 A1 SG 147349A1 SG 2007032386 A SG2007032386 A SG 2007032386A SG 147349 A1 SG147349 A1 SG 147349A1
Authority
SG
Singapore
Prior art keywords
nucleic acid
sample
target nucleic
reaction
well
Prior art date
Application number
SG200703238-6A
Inventor
Martin Alan Lee
Hilary Bird
Dario Lyall Leslie
David James Squirrell
John Shaw
David Wenn
Julie Deacon
Original Assignee
Secr Defence
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Secr Defence filed Critical Secr Defence
Priority to SG200703238-6A priority Critical patent/SG147349A1/en
Publication of SG147349A1 publication Critical patent/SG147349A1/en

Links

Abstract

Reaction System A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined there between; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the p.H. is above 8.3; 8) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions.
SG200703238-6A 2007-05-04 2007-05-04 Reaction system SG147349A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SG200703238-6A SG147349A1 (en) 2007-05-04 2007-05-04 Reaction system

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SG200703238-6A SG147349A1 (en) 2007-05-04 2007-05-04 Reaction system

Publications (1)

Publication Number Publication Date
SG147349A1 true SG147349A1 (en) 2008-11-28

Family

ID=40032357

Family Applications (1)

Application Number Title Priority Date Filing Date
SG200703238-6A SG147349A1 (en) 2007-05-04 2007-05-04 Reaction system

Country Status (1)

Country Link
SG (1) SG147349A1 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001023093A1 (en) * 1999-09-29 2001-04-05 The Secretary Of State For Defence Reaction system for performing in the amplification of nucleic acids

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001023093A1 (en) * 1999-09-29 2001-04-05 The Secretary Of State For Defence Reaction system for performing in the amplification of nucleic acids

Similar Documents

Publication Publication Date Title
GB2369592A (en) Reaction system for performing in the amplification of nucleic acids
EP3286338B1 (en) Amplification with primers of limited nucleotide composition
US9737887B2 (en) Integrated sample preparation systems and stabilized enzyme mixtures
EP2294225B1 (en) Method for direct amplification from crude nucleic acid samples
WO2005083068A3 (en) Methods for obtaining thermostable enzymes, dna polymerase i variants from thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications to the same
EP1727911A2 (en) Base specific cleavage of methylation-specific amplification products in combination with mass analysis
JP2012231799A (en) Method for amplifying and sequencing nucleic acid
JP2008533983A (en) Polymorphism detection method
WO2007127992A3 (en) Use of base-modified deoxynucleoside triphosphates
WO2017161306A1 (en) Improved amplification and sequencing methods
US8153401B2 (en) Method for direct amplification from crude nucleic acid samples
GB0223563D0 (en) Detection system
CA2655518A1 (en) Method for analyzing amplified nucleic acids
EP2971140B1 (en) Methods to assess contamination in dna sequencing
CA3140836A1 (en) A method of removing amplicons of a non-target nucleic acid having one or more methylated cytosines from a sample
SG147349A1 (en) Reaction system
US20110045485A1 (en) Analytical method and kit
ES2305233T3 (en) PROCEDURE AND ANALYTICAL EQUIPMENT.
Jin et al. Real-time monitoring of DNA methyltransferase activity using a hemimethylated smart probe
Huang et al. Allele frequencies and statistic parameters for penta D and penta E loci in Chinese Han population
AU2014259546A1 (en) Integrated sample preparation systems and stabilized enzyme mixtures
Sarno Genomic 8-oxo-7, 8-dihydro-2'-deoxyguanosine Quantification
Edition PCR Technology