SG147349A1 - Reaction system - Google Patents
Reaction systemInfo
- Publication number
- SG147349A1 SG147349A1 SG200703238-6A SG2007032386A SG147349A1 SG 147349 A1 SG147349 A1 SG 147349A1 SG 2007032386 A SG2007032386 A SG 2007032386A SG 147349 A1 SG147349 A1 SG 147349A1
- Authority
- SG
- Singapore
- Prior art keywords
- nucleic acid
- sample
- target nucleic
- reaction
- well
- Prior art date
Links
Abstract
Reaction System A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined there between; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the p.H. is above 8.3; 8) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SG200703238-6A SG147349A1 (en) | 2007-05-04 | 2007-05-04 | Reaction system |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SG200703238-6A SG147349A1 (en) | 2007-05-04 | 2007-05-04 | Reaction system |
Publications (1)
Publication Number | Publication Date |
---|---|
SG147349A1 true SG147349A1 (en) | 2008-11-28 |
Family
ID=40032357
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG200703238-6A SG147349A1 (en) | 2007-05-04 | 2007-05-04 | Reaction system |
Country Status (1)
Country | Link |
---|---|
SG (1) | SG147349A1 (en) |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001023093A1 (en) * | 1999-09-29 | 2001-04-05 | The Secretary Of State For Defence | Reaction system for performing in the amplification of nucleic acids |
-
2007
- 2007-05-04 SG SG200703238-6A patent/SG147349A1/en unknown
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001023093A1 (en) * | 1999-09-29 | 2001-04-05 | The Secretary Of State For Defence | Reaction system for performing in the amplification of nucleic acids |
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