SG11202112036YA - Cultivation systems and methods for large-scale production of cultured food - Google Patents

Cultivation systems and methods for large-scale production of cultured food

Info

Publication number
SG11202112036YA
SG11202112036YA SG11202112036YA SG11202112036YA SG11202112036YA SG 11202112036Y A SG11202112036Y A SG 11202112036YA SG 11202112036Y A SG11202112036Y A SG 11202112036YA SG 11202112036Y A SG11202112036Y A SG 11202112036YA SG 11202112036Y A SG11202112036Y A SG 11202112036YA
Authority
SG
Singapore
Prior art keywords
methods
scale production
cultivation systems
cultured food
cultured
Prior art date
Application number
SG11202112036YA
Inventor
Neta Lavon
Moshe Aviv
Yoav Roth
Didier Toubia
Original Assignee
Aleph Farms Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aleph Farms Ltd filed Critical Aleph Farms Ltd
Publication of SG11202112036YA publication Critical patent/SG11202112036YA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/26—Constructional details, e.g. recesses, hinges flexible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/58—Reaction vessels connected in series or in parallel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16—Vibrating; Shaking; Tilting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0653—Adipocytes; Adipose tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0658—Skeletal muscle cells, e.g. myocytes, myotubes, myoblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
SG11202112036YA 2019-05-02 2020-04-30 Cultivation systems and methods for large-scale production of cultured food SG11202112036YA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962841864P 2019-05-02 2019-05-02
PCT/IL2020/050484 WO2020222239A1 (en) 2019-05-02 2020-04-30 Cultivation systems and methods for large-scale production of cultured food

Publications (1)

Publication Number Publication Date
SG11202112036YA true SG11202112036YA (en) 2021-11-29

Family

ID=73029703

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202112036YA SG11202112036YA (en) 2019-05-02 2020-04-30 Cultivation systems and methods for large-scale production of cultured food

Country Status (7)

Country Link
US (1) US20220195359A1 (en)
EP (1) EP3963052A4 (en)
JP (1) JP2022532498A (en)
CN (1) CN114207115A (en)
IL (1) IL287620A (en)
SG (1) SG11202112036YA (en)
WO (1) WO2020222239A1 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020123876A1 (en) * 2018-12-12 2020-06-18 Wild Type, Inc. Synthetic food compositions
PE20230409A1 (en) * 2019-12-31 2023-03-07 Air Protein Inc FOOD COMPOSITIONS WITH HIGH PROTEIN CONTENT
WO2022038241A1 (en) 2020-08-21 2022-02-24 Merck Patent Gmbh Process system for bioreactor-based clean meat production
EP4240173A1 (en) * 2020-11-03 2023-09-13 Aleph Farms Ltd. Edible protein products
JP2023548434A (en) * 2020-11-04 2023-11-16 アレフ ファームス リミテッド Multi-scaffold system for large-scale culture of cells
CN112831416A (en) * 2021-01-29 2021-05-25 上海睿钰生物科技有限公司 In-vitro life-sustaining perfusion culture system and control method thereof
WO2022189505A1 (en) 2021-03-09 2022-09-15 Ants Innovate Pte. Ltd. Scalable methods for manufacturing alternative meat cuts
WO2022208525A1 (en) * 2021-03-31 2022-10-06 Myoworks Pvt. Ltd. A scaffolding for cultivated meat and a process for making the same
IL308773A (en) * 2021-06-16 2024-01-01 Upside Foods Inc Plant fat-based scaffolds for the growth of cell-based meats and methods of making such products
US20220403309A1 (en) 2021-06-16 2022-12-22 Upside Foods, Inc. Plant fat-based scaffolds for the growth of cell-based meats and methods of making such products
WO2023031839A2 (en) * 2021-09-01 2023-03-09 Sophie's Bionutrients Pte Ltd. Using mixotrophic microalgae to produce cultured meat and seafood
CA3231125A1 (en) 2021-10-19 2023-04-27 Joshua MARCH Plant base/animal cell hybrid meat substitute
US20230203446A1 (en) * 2021-12-29 2023-06-29 Upside Foods, Inc. Method for washing and finishing a grown cell mass
US11912972B2 (en) * 2022-04-25 2024-02-27 Ark Biotech Inc. Scaffold bioreactor
WO2024024967A1 (en) * 2022-07-29 2024-02-01 日機装株式会社 Culture system
WO2024048588A1 (en) * 2022-08-31 2024-03-07 インテグリカルチャー株式会社 Cell culture system, cultured meat, and culture supernatant

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999031222A1 (en) * 1997-12-18 1999-06-24 Willem Frederik Van Eelen Industrial scale production of meat from in vitro cell cultures
GB0718245D0 (en) * 2007-09-19 2007-10-31 Univ Bath Bioreactors for tissue engineering
WO2015003012A2 (en) * 2013-07-01 2015-01-08 Olivier Berteau Distributed perfusion bioreactor system for continuous culture of biological cells
CN109071594A (en) * 2016-04-14 2018-12-21 崔泽尔有限公司 With constant flow pump/pipe-line system fixed-bed bioreactor
WO2018011805A2 (en) * 2016-07-11 2018-01-18 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Systems and methods for growing cells in vitro

Also Published As

Publication number Publication date
US20220195359A1 (en) 2022-06-23
EP3963052A4 (en) 2023-05-24
CN114207115A (en) 2022-03-18
JP2022532498A (en) 2022-07-15
IL287620A (en) 2021-12-01
WO2020222239A1 (en) 2020-11-05
EP3963052A1 (en) 2022-03-09

Similar Documents

Publication Publication Date Title
IL287620A (en) Cultivation systems and methods for large-scale production of cultured food
ZA202004233B (en) Reactors and submerged fermentation methods for producing microbe-based products
EP3612623A4 (en) Compositions and methods for increasing the culture density of a cellular biomass within a cultivation infrastructure
EP3106515A4 (en) Method for preparing fermentation broth extracts having chymosin activity and products thereof
EP3843529A4 (en) Plant cultivation device and method for culturing plant
EP3584313A4 (en) Maintenance culture of intestinal stem cells derived from induced pluripotent stem cells
EP3510140A4 (en) Novel cultivation system for the efficient production of microorganisms
EP3721979A4 (en) Charged nanobubble dispersion, production method for charged nanobubble dispersion, production device for charged nanobubble dispersion, and method for using charged nanobubble dispersion to control growth rate of microorganisms and plants
MY193225A (en) Methods for continuous production of products from microorganisms
EP3508566A4 (en) Method for culturing photosynthetic microalgae
MX2019002223A (en) Improved method for growing algae.
IL290068B1 (en) System and method for seeding and culturing
PL3466854T3 (en) Method and plant for producing logs of thin products
EP3146058A4 (en) Fermentation process for the production and control of pyruvate-derived products
PL3737223T3 (en) Nutrient medium for automated cultivation of plants
GB202200070D0 (en) Method of culture
EP3702444A4 (en) Method for inducing differentiation of pluripotent stem cells into hepatocytes
EP3765597A4 (en) Bacterial cultures and methods for production of alginate
EP3167043A4 (en) Bioreactor for production and harvesting of microalgae
HK1250048A1 (en) Method and bioreactor for producing a culture of microorganisms
ZA202102842B (en) Culture medium for improving induction rate of tissue cultured plant, preparation method, and application
EP3689134A4 (en) Method for producing plants and method for producing processed plant products
IL281231A (en) Methods of continuous cell culture
EP3694982C0 (en) Method and system for heterotrophic and mixotrophic cultivation of microalgae
GB201707034D0 (en) Modified microorganisms and methods for production of useful products