SG11202109391PA - Method for the generation of an fcrn expressing cell by targeted integration of multiple expression cassettes in a defined organization - Google Patents

Method for the generation of an fcrn expressing cell by targeted integration of multiple expression cassettes in a defined organization

Info

Publication number
SG11202109391PA
SG11202109391PA SG11202109391PA SG11202109391PA SG11202109391PA SG 11202109391P A SG11202109391P A SG 11202109391PA SG 11202109391P A SG11202109391P A SG 11202109391PA SG 11202109391P A SG11202109391P A SG 11202109391PA SG 11202109391P A SG11202109391P A SG 11202109391PA
Authority
SG
Singapore
Prior art keywords
generation
expression cassettes
expressing cell
targeted integration
multiple expression
Prior art date
Application number
SG11202109391PA
Inventor
Tilman Schlothauer
Stefan Seeber
Jasmin Maria Wehrstein
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of SG11202109391PA publication Critical patent/SG11202109391PA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70535—Fc-receptors, e.g. CD16, CD32, CD64 (CD2314/705F)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70539—MHC-molecules, e.g. HLA-molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/04—Other carbon-nitrogen ligases (6.3.4)
    • C12Y603/04015—Biotin-[acetyl-CoA-carboxylase] ligase (6.3.4.15)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/22—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a Strep-tag

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
SG11202109391PA 2019-03-29 2020-03-26 Method for the generation of an fcrn expressing cell by targeted integration of multiple expression cassettes in a defined organization SG11202109391PA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP19166030 2019-03-29
PCT/EP2020/058452 WO2020200983A1 (en) 2019-03-29 2020-03-26 Method for the generation of an fcrn expressing cell by targeted integration of multiple expression cassettes in a defined organization

Publications (1)

Publication Number Publication Date
SG11202109391PA true SG11202109391PA (en) 2021-09-29

Family

ID=66239740

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202109391PA SG11202109391PA (en) 2019-03-29 2020-03-26 Method for the generation of an fcrn expressing cell by targeted integration of multiple expression cassettes in a defined organization

Country Status (12)

Country Link
US (1) US20220119482A1 (en)
EP (1) EP3947437A1 (en)
JP (1) JP7250950B2 (en)
KR (1) KR20210134932A (en)
CN (1) CN113661173A (en)
AU (1) AU2020253023B2 (en)
BR (1) BR112021019368A2 (en)
CA (1) CA3130546A1 (en)
IL (1) IL286718A (en)
MX (1) MX2021011837A (en)
SG (1) SG11202109391PA (en)
WO (1) WO2020200983A1 (en)

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2096222C (en) 1990-11-13 1998-12-29 Stephen D. Lupton Bifunctional selectable fusion genes
EP0804590A1 (en) 1993-05-21 1997-11-05 Targeted Genetics Corporation Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene
EA014182B1 (en) 2004-07-20 2010-10-29 Симфоген А/С Anti-rhesus d recombinant polyclonal antibody composition, method for manufacturing thereof and use thereof
WO2007124452A2 (en) * 2006-04-20 2007-11-01 The Regents Of The University Of California Methods for expressing multiple sirna and shrna from a single vector
US8293533B2 (en) * 2008-12-19 2012-10-23 E.I. Du Pont De Nemours And Company Site-specific integration and stacking of transgenes in soybean via DNA recombinase mediated cassette exchange
WO2013006142A1 (en) 2011-07-05 2013-01-10 Nanyang Technological University A novel process and reagent for rapid genetic alterations in eukaryotic cells
RU2624128C2 (en) * 2012-02-15 2017-06-30 Ф. Хоффманн-Ля Рош Аг AFFINITY CHROMATOGRAPHY WITH APPLICATION OF Fc-RECEPTORS
WO2014162318A2 (en) * 2013-03-30 2014-10-09 Usha Biotech Limited Methods and constructs for expressing biologically active proteins in mammalian cells
HK1218486A2 (en) * 2015-12-09 2017-02-17 Ka Lun Alan Ng Means and method for enhancing growth of fish
BR112018071285A2 (en) * 2016-04-20 2019-02-12 Regeneron Pharma cell, vector set, vector, system, and method
AU2018232698B2 (en) 2017-03-10 2020-06-25 F. Hoffmann-La Roche Ag Method for producing multispecific antibodies
WO2018197533A1 (en) * 2017-04-28 2018-11-01 F. Hoffmann-La Roche Ag Antibody selection method
AU2018392728A1 (en) 2017-12-22 2020-07-09 Genentech, Inc. Targeted integration of nucleic acids

Also Published As

Publication number Publication date
US20220119482A1 (en) 2022-04-21
KR20210134932A (en) 2021-11-11
EP3947437A1 (en) 2022-02-09
IL286718A (en) 2021-10-31
CN113661173A (en) 2021-11-16
AU2020253023A1 (en) 2021-09-23
CA3130546A1 (en) 2020-10-08
AU2020253023B2 (en) 2022-07-14
BR112021019368A2 (en) 2021-12-07
WO2020200983A1 (en) 2020-10-08
MX2021011837A (en) 2021-10-22
JP2022526556A (en) 2022-05-25
JP7250950B2 (en) 2023-04-03

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