SG11202102003TA - Methods of generating an array - Google Patents

Methods of generating an array

Info

Publication number
SG11202102003TA
SG11202102003TA SG11202102003TA SG11202102003TA SG11202102003TA SG 11202102003T A SG11202102003T A SG 11202102003TA SG 11202102003T A SG11202102003T A SG 11202102003TA SG 11202102003T A SG11202102003T A SG 11202102003TA SG 11202102003T A SG11202102003T A SG 11202102003TA
Authority
SG
Singapore
Prior art keywords
array
generating
methods
Prior art date
Application number
SG11202102003TA
Inventor
Michael Schnall-Levin
Lucas Frenz
Original Assignee
10X Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 10X Genomics Inc filed Critical 10X Genomics Inc
Publication of SG11202102003TA publication Critical patent/SG11202102003TA/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6841In situ hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Lubricants (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
SG11202102003TA 2018-08-28 2019-08-27 Methods of generating an array SG11202102003TA (en)

Applications Claiming Priority (43)

Application Number Priority Date Filing Date Title
US201862723950P 2018-08-28 2018-08-28
US201862723964P 2018-08-28 2018-08-28
US201862723957P 2018-08-28 2018-08-28
US201862723972P 2018-08-28 2018-08-28
US201862723960P 2018-08-28 2018-08-28
US201862723970P 2018-08-28 2018-08-28
US201862724487P 2018-08-29 2018-08-29
US201862724483P 2018-08-29 2018-08-29
US201862724561P 2018-08-29 2018-08-29
US201862724489P 2018-08-29 2018-08-29
US201862779342P 2018-12-13 2018-12-13
US201962788885P 2019-01-06 2019-01-06
US201962788905P 2019-01-06 2019-01-06
US201962788867P 2019-01-06 2019-01-06
US201962788871P 2019-01-06 2019-01-06
US201962788897P 2019-01-06 2019-01-06
US201962812219P 2019-02-28 2019-02-28
US201962819449P 2019-03-15 2019-03-15
US201962819496P 2019-03-15 2019-03-15
US201962819456P 2019-03-15 2019-03-15
US201962819448P 2019-03-15 2019-03-15
US201962819458P 2019-03-15 2019-03-15
US201962819468P 2019-03-15 2019-03-15
US201962819486P 2019-03-15 2019-03-15
US201962819478P 2019-03-15 2019-03-15
US201962819467P 2019-03-15 2019-03-15
US201962822592P 2019-03-22 2019-03-22
US201962822565P 2019-03-22 2019-03-22
US201962822554P 2019-03-22 2019-03-22
US201962822618P 2019-03-22 2019-03-22
US201962822627P 2019-03-22 2019-03-22
US201962822649P 2019-03-22 2019-03-22
US201962822605P 2019-03-22 2019-03-22
US201962822575P 2019-03-22 2019-03-22
US201962822632P 2019-03-22 2019-03-22
US201962839223P 2019-04-26 2019-04-26
US201962839346P 2019-04-26 2019-04-26
US201962839526P 2019-04-26 2019-04-26
US201962839320P 2019-04-26 2019-04-26
US201962842463P 2019-05-02 2019-05-02
US201962858331P 2019-06-07 2019-06-07
US201962860993P 2019-06-13 2019-06-13
PCT/US2019/048427 WO2020047004A2 (en) 2018-08-28 2019-08-27 Methods of generating an array

Publications (1)

Publication Number Publication Date
SG11202102003TA true SG11202102003TA (en) 2021-03-30

Family

ID=69643282

Family Applications (4)

Application Number Title Priority Date Filing Date
SG11202102029TA SG11202102029TA (en) 2018-08-28 2019-08-27 Methods for generating spatially barcoded arrays
SG11202102019UA SG11202102019UA (en) 2018-08-28 2019-08-27 Method for transposase-mediated spatial tagging and analyzing genomic dna in a biological sample
SG11202101944RA SG11202101944RA (en) 2018-08-28 2019-08-27 Increasing spatial array resolution
SG11202102003TA SG11202102003TA (en) 2018-08-28 2019-08-27 Methods of generating an array

Family Applications Before (3)

Application Number Title Priority Date Filing Date
SG11202102029TA SG11202102029TA (en) 2018-08-28 2019-08-27 Methods for generating spatially barcoded arrays
SG11202102019UA SG11202102019UA (en) 2018-08-28 2019-08-27 Method for transposase-mediated spatial tagging and analyzing genomic dna in a biological sample
SG11202101944RA SG11202101944RA (en) 2018-08-28 2019-08-27 Increasing spatial array resolution

Country Status (5)

Country Link
US (5) US20210332425A1 (en)
EP (4) EP3844306A2 (en)
CN (3) CN113286893A (en)
SG (4) SG11202102029TA (en)
WO (4) WO2020047007A2 (en)

Families Citing this family (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
PT2556171E (en) 2010-04-05 2015-12-21 Prognosys Biosciences Inc Spatially encoded biological assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
CN105849275B (en) 2013-06-25 2020-03-17 普罗格诺西斯生物科学公司 Method and system for detecting spatial distribution of biological targets in a sample
EP3530752B1 (en) 2015-04-10 2021-03-24 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
EP3788171B1 (en) 2018-05-03 2023-04-05 Becton, Dickinson and Company High throughput multiomics sample analysis
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
EP3894585A2 (en) 2018-12-10 2021-10-20 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US20240026445A1 (en) * 2019-01-06 2024-01-25 10X Genomics, Inc. Methods for determining a location of a biological analyte in a biological sample
CN113631764A (en) * 2019-02-21 2021-11-09 斯特拉托斯基因公司 Methods, compositions, and apparatus for solid state synthesis of scalable polymers for use in single molecule sequencing
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
WO2021097255A1 (en) * 2019-11-13 2021-05-20 10X Genomics, Inc. Generating capture probes for spatial analysis
CN117746422A (en) * 2019-11-22 2024-03-22 10X基因组学有限公司 System and method for spatially analyzing analytes using fiducial alignment
EP4073241A2 (en) 2019-12-11 2022-10-19 10X Genomics, Inc. Reverse transcriptase variants
SG11202106899SA (en) 2019-12-23 2021-09-29 10X Genomics Inc Methods for spatial analysis using rna-templated ligation
JP2023511279A (en) 2020-01-13 2023-03-17 フルーエント バイオサイエンシーズ インコーポレイテッド Single cell sequencing
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
WO2021168278A1 (en) * 2020-02-20 2021-08-26 10X Genomics, Inc. METHODS TO COMBINE FIRST AND SECOND STRAND cDNA SYNTHESIS FOR SPATIAL ANALYSIS
EP4107285A1 (en) 2020-02-21 2022-12-28 10X Genomics, Inc. Methods and compositions for integrated in situ spatial assay
EP4107282A1 (en) * 2020-02-21 2022-12-28 10X Genomics, Inc. Capturing genetic targets using a hybridization approach
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
EP4121016A1 (en) 2020-03-16 2023-01-25 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
EP4136227A1 (en) 2020-04-16 2023-02-22 10X Genomics, Inc. Compositions and methods for use with fixed samples
EP4242325A3 (en) 2020-04-22 2023-10-04 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
US20230265491A1 (en) * 2020-05-04 2023-08-24 10X Genomics, Inc. Spatial transcriptomic transfer modes
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
EP4153775A1 (en) 2020-05-22 2023-03-29 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
EP4153776A1 (en) 2020-05-22 2023-03-29 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
CN116249785A (en) 2020-06-02 2023-06-09 10X基因组学有限公司 Space transcriptomics for antigen-receptor
EP4025692A2 (en) 2020-06-02 2022-07-13 10X Genomics, Inc. Nucleic acid library methods
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4165207A1 (en) 2020-06-10 2023-04-19 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
AU2021294334A1 (en) 2020-06-25 2023-02-02 10X Genomics, Inc. Spatial analysis of DNA methylation
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11802312B2 (en) 2020-08-10 2023-10-31 Dimension Genomics Inc. Devices and methods for multi-dimensional genome analysis
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
EP4213993A2 (en) * 2020-09-18 2023-07-26 10X Genomics, Inc. Sample handling apparatus and fluid delivery methods
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
CN112143784A (en) * 2020-09-29 2020-12-29 生物岛实验室 Space omics sequencing, single-cell apparent transcriptomics sequencing and positioning identification method
WO2022067565A1 (en) * 2020-09-29 2022-04-07 生物岛实验室 Spatial omics sequencing method, single cell epitranscriptomic sequencing method, and localization labeling method
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
WO2022132645A1 (en) * 2020-12-14 2022-06-23 Chan Zuckerberg Biohub, Inc. Spatial genomics with single cell resolution
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US20220314187A1 (en) * 2020-12-30 2022-10-06 10X Genomics, Inc. Methods and compositions for light-controlled surface patterning using a polymer
EP4271804A1 (en) * 2020-12-30 2023-11-08 Dovetail Genomics, LLC Methods and compositions for sequencing library preparation
WO2022147296A1 (en) 2020-12-30 2022-07-07 10X Genomics, Inc. Cleavage of capture probes for spatial analysis
EP4271509A1 (en) * 2020-12-30 2023-11-08 10X Genomics, Inc. Molecular arrays and methods for generating and using the arrays
US20240060122A1 (en) * 2021-01-12 2024-02-22 Paris Sciences Et Lettres Method for spatial mapping and sequencing of cells or organelles
WO2022198068A1 (en) 2021-03-18 2022-09-22 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
WO2022221425A1 (en) * 2021-04-14 2022-10-20 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
WO2022226057A1 (en) * 2021-04-20 2022-10-27 10X Genomics, Inc. Methods for assessing sample quality prior to spatial analysis using templated ligation
WO2022226372A1 (en) * 2021-04-23 2022-10-27 Ultima Genomics, Inc. Systems and methods for spatial reference sequencing
WO2022232571A1 (en) 2021-04-30 2022-11-03 10X Genomics, Inc. Fusion rt variants for improved performance
WO2022251419A1 (en) * 2021-05-26 2022-12-01 Board Of Regents, The University Of Texas System Methods and systems for single cell protein analysis
WO2022251445A1 (en) * 2021-05-28 2022-12-01 Fluent Biosciences Inc. Spatial transcriptomics in pips
EP4347879A1 (en) * 2021-06-03 2024-04-10 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
WO2022265965A1 (en) 2021-06-14 2022-12-22 10X Genomics, Inc. Reverse transcriptase variants for improved performance
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2023059658A1 (en) * 2021-10-04 2023-04-13 AtlasXomics Inc. System and methods for high throughput screening of tissue preparation conditions
WO2023076345A1 (en) * 2021-10-26 2023-05-04 10X Genomics, Inc. Methods for spatial analysis using targeted rna capture
WO2023114473A2 (en) 2021-12-16 2023-06-22 10X Genomics, Inc. Recombinant reverse transcriptase variants for improved performance
WO2023116938A1 (en) * 2021-12-24 2023-06-29 映泰科技有限公司 Biochip for spatial transcriptomic analysis, and preparation method and application thereof
CN116694730A (en) * 2022-02-28 2023-09-05 南方科技大学 Construction method of single cell open chromatin and transcriptome co-sequencing library
CN114574569B (en) * 2022-03-28 2023-05-16 浙江大学 Genome sequencing kit and sequencing method based on terminal transferase
WO2023196526A1 (en) 2022-04-06 2023-10-12 10X Genomics, Inc. Methods for multiplex cell analysis
WO2023215612A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Analysis of antigen and antigen receptor interactions
WO2023225519A1 (en) 2022-05-17 2023-11-23 10X Genomics, Inc. Modified transposons, compositions and uses thereof
WO2023250077A1 (en) * 2022-06-22 2023-12-28 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
WO2024006799A1 (en) * 2022-06-29 2024-01-04 10X Genomics, Inc. Covalent attachment of splint oligonucleotides for molecular array generation using ligation
WO2024006832A1 (en) * 2022-06-29 2024-01-04 10X Genomics, Inc. Click chemistry-based dna photo-ligation for manufacturing of high-resolution dna arrays

Family Cites Families (135)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US4968601A (en) * 1988-02-09 1990-11-06 The United States Of America As Represented By The Dept. Of Health & Human Services Method for diagnosing latent viral infection
US5472881A (en) 1992-11-12 1995-12-05 University Of Utah Research Foundation Thiol labeling of DNA for attachment to gold surfaces
DE69434520T3 (en) 1993-07-30 2009-10-15 Affymax, Inc., Palo Alto BIOTINYLATION OF PROTEINS
US5610287A (en) 1993-12-06 1997-03-11 Molecular Tool, Inc. Method for immobilizing nucleic acid molecules
US5512462A (en) 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
US5552278A (en) 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5925545A (en) 1996-09-09 1999-07-20 Wisconsin Alumni Research Foundation System for in vitro transposition
US5965443A (en) 1996-09-09 1999-10-12 Wisconsin Alumni Research Foundation System for in vitro transposition
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
EP0968223B1 (en) 1997-01-08 2016-12-21 Sigma-Aldrich Co. LLC Bioconjugation of macromolecules
US5837860A (en) 1997-03-05 1998-11-17 Molecular Tool, Inc. Covalent attachment of nucleic acid molecules onto solid-phases via disulfide bonds
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
US6023540A (en) 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
ATE269908T1 (en) 1997-04-01 2004-07-15 Manteia S A METHOD FOR SEQUENCING NUCLEIC ACIDS
AU6846698A (en) 1997-04-01 1998-10-22 Glaxo Group Limited Method of nucleic acid amplification
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
US5919626A (en) 1997-06-06 1999-07-06 Orchid Bio Computer, Inc. Attachment of unmodified nucleic acids to silanized solid phase surfaces
US7427678B2 (en) 1998-01-08 2008-09-23 Sigma-Aldrich Co. Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method
US6387632B2 (en) * 1998-06-11 2002-05-14 Hitachi, Ltd. Polynucleotide separation method and apparatus therefor
EP2045334A1 (en) 1998-06-24 2009-04-08 Illumina, Inc. Decoding of array sensors with microspheres
US6159736A (en) 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
AR021833A1 (en) 1998-09-30 2002-08-07 Applied Research Systems METHODS OF AMPLIFICATION AND SEQUENCING OF NUCLEIC ACID
US6391937B1 (en) 1998-11-25 2002-05-21 Motorola, Inc. Polyacrylamide hydrogels and hydrogel arrays made from polyacrylamide reactive prepolymers
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
ATE413467T1 (en) 1999-04-20 2008-11-15 Illumina Inc DETECTION OF NUCLEIC ACID REACTIONS ON BEAD ARRAYS
US20060275782A1 (en) * 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
WO2001009363A1 (en) 1999-08-02 2001-02-08 Wisconsin Alumni Research Foundation Mutant tn5 transposase enzymes and method for their use
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
GB0018120D0 (en) 2000-07-24 2000-09-13 Fermentas Ab Nuclease
AR031640A1 (en) 2000-12-08 2003-09-24 Applied Research Systems ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS IN A SOLID SUPPORT
US7297778B2 (en) * 2001-07-25 2007-11-20 Affymetrix, Inc. Complexity management of genomic DNA
US20040019005A1 (en) * 2001-10-01 2004-01-29 Jeffrey Van Ness Methods for parallel measurement of genetic variations
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
WO2003070294A2 (en) 2002-01-16 2003-08-28 Mayo Foundation For Medical Education And Research Method and apparatus for image-guided therapy
US20040002090A1 (en) 2002-03-05 2004-01-01 Pascal Mayer Methods for detecting genome-wide sequence variations associated with a phenotype
KR101138643B1 (en) 2002-05-30 2012-04-26 더 스크립스 리서치 인스티튜트 Copper-catalysed ligation of azides and acetylenes
SI3002289T1 (en) 2002-08-23 2018-07-31 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
KR20040062847A (en) * 2003-01-03 2004-07-09 삼성전자주식회사 Method for replicating nucleic acid array
GB0302058D0 (en) * 2003-01-29 2003-02-26 Univ Cranfield Replication of nucleic acid arrays
US7083980B2 (en) 2003-04-17 2006-08-01 Wisconsin Alumni Research Foundation Tn5 transposase mutants and the use thereof
US20050031176A1 (en) 2003-08-08 2005-02-10 Hertel Sarah R. Method and apparatus of multi-modality image fusion
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
US7259258B2 (en) 2003-12-17 2007-08-21 Illumina, Inc. Methods of attaching biological compounds to solid supports using triazine
JP2007525571A (en) 2004-01-07 2007-09-06 ソレクサ リミテッド Modified molecular array
US7608434B2 (en) 2004-08-04 2009-10-27 Wisconsin Alumni Research Foundation Mutated Tn5 transposase proteins and the use thereof
GB0427236D0 (en) 2004-12-13 2005-01-12 Solexa Ltd Improved method of nucleotide detection
WO2006064199A1 (en) 2004-12-13 2006-06-22 Solexa Limited Improved method of nucleotide detection
CN109485727A (en) * 2005-05-09 2019-03-19 小野药品工业株式会社 The human monoclonal antibodies of programmed death-1 (PD-1) and the method for carrying out treating cancer using anti-PD-1 antibody
EP1888743B1 (en) 2005-05-10 2011-08-03 Illumina Cambridge Limited Improved polymerases
ES2565670T3 (en) 2005-06-20 2016-04-06 Advanced Cell Diagnostics, Inc. Nucleic acid detection methods in individual cells and identification of rare cells in large populations of heterogeneous cells
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
EP2021503A1 (en) 2006-03-17 2009-02-11 Solexa Ltd. Isothermal methods for creating clonal single molecule arrays
US20070231823A1 (en) * 2006-03-23 2007-10-04 Mckernan Kevin J Directed enrichment of genomic DNA for high-throughput sequencing
EP3536396B1 (en) 2006-08-07 2022-03-30 The President and Fellows of Harvard College Fluorocarbon emulsion stabilizing surfactants
WO2008022332A2 (en) * 2006-08-18 2008-02-21 Board Of Regents, The University Of Texas System System, method and kit for replicating a dna array
ES2923759T3 (en) 2006-12-14 2022-09-30 Life Technologies Corp Apparatus for measuring analytes using FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US20100055733A1 (en) 2008-09-04 2010-03-04 Lutolf Matthias P Manufacture and uses of reactive microcontact printing of biomolecules on soft hydrogels
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
ES2719502T3 (en) 2010-01-29 2019-07-10 Advanced Cell Diagnostics Inc Nucleic acid in situ detection methods
EP2354242A1 (en) * 2010-02-03 2011-08-10 Epiontis GmbH Assay for determining the type and/or status of a cell based on the epigenetic pattern and the chromatin structure
PT2556171E (en) 2010-04-05 2015-12-21 Prognosys Biosciences Inc Spatially encoded biological assays
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP2739752B1 (en) * 2011-08-03 2017-07-19 Bio-Rad Laboratories, Inc. Filtering small nucleic acids using permeabilized cells
DK3623481T3 (en) 2011-09-23 2021-11-15 Illumina Inc COMPOSITIONS FOR NUCLEIC ACID SEQUENCE
WO2013096838A2 (en) * 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids
US9193996B2 (en) 2012-04-03 2015-11-24 Illumina, Inc. Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
CA2881685C (en) 2012-08-14 2023-12-05 10X Genomics, Inc. Microcapsule compositions and methods
US20140378345A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US9783841B2 (en) 2012-10-04 2017-10-10 The Board Of Trustees Of The Leland Stanford Junior University Detection of target nucleic acids in a cellular sample
CA2886974C (en) 2012-10-17 2021-06-29 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
WO2014085725A1 (en) 2012-11-27 2014-06-05 Tufts University Biopolymer-based inks and use thereof
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
EP3578666A1 (en) 2013-03-12 2019-12-11 President and Fellows of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
WO2014149629A1 (en) * 2013-03-15 2014-09-25 Htg Molecular Diagnostics, Inc. Subtyping lung cancers
CN105849275B (en) 2013-06-25 2020-03-17 普罗格诺西斯生物科学公司 Method and system for detecting spatial distribution of biological targets in a sample
US20150000854A1 (en) 2013-06-27 2015-01-01 The Procter & Gamble Company Sheet products bearing designs that vary among successive sheets, and apparatus and methods for producing the same
CN105658310A (en) 2013-09-13 2016-06-08 斯坦福大学托管董事会 Multiplexed imaging of tissues using mass tags and secondary ion mass spectrometry
WO2015094123A1 (en) * 2013-12-20 2015-06-25 National University Of Singapore Differentiation therapy with cd137 ligand agonists
CN106460069B (en) 2014-04-18 2021-02-12 威廉马歇莱思大学 Competitive compositions for enriching nucleic acid molecules for rare allele-containing material
CA2951325C (en) 2014-06-06 2023-09-12 Herlev Hospital Determining antigen recognition through barcoding of mhc multimers
US11585806B2 (en) 2014-06-13 2023-02-21 Immudex Aps General detection and isolation of specific cells by binding of labeled molecules
JP6838969B2 (en) 2014-06-26 2021-03-03 10エックス ジェノミクス, インコーポレイテッド Method for Analyzing Nucleic Acids Derived from Individual Cells or Cell Populations
WO2016007839A1 (en) 2014-07-11 2016-01-14 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
KR20170064540A (en) * 2014-09-26 2017-06-09 투 포어 가이즈, 인코포레이티드 Target sequence detection by nanopore sensing of synthetic probes
US20160108458A1 (en) 2014-10-06 2016-04-21 The Board Of Trustees Of The Leland Stanford Junior University Multiplexed detection and quantification of nucleic acids in single-cells
ES2836802T3 (en) 2015-02-27 2021-06-28 Becton Dickinson Co Spatially addressable molecular barcodes
ES2934982T3 (en) * 2015-03-30 2023-02-28 Becton Dickinson Co Methods for encoding with combinatorial barcodes
EP3530752B1 (en) * 2015-04-10 2021-03-24 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US10059990B2 (en) 2015-04-14 2018-08-28 Massachusetts Institute Of Technology In situ nucleic acid sequencing of expanded biological samples
CN107709574B (en) 2015-04-14 2021-10-01 皇家飞利浦有限公司 Spatial mapping of molecular profiles of biological tissue samples
JP6971218B2 (en) 2015-07-17 2021-11-24 ナノストリング テクノロジーズ,インコーポレイティド Simultaneous quantification of gene expression in user-defined regions of cross-tissue sections
CA3176469A1 (en) 2015-07-27 2017-02-02 Illumina, Inc. Spatial mapping of nucleic acid sequence information
CN108139408B (en) 2015-08-07 2020-08-28 麻省理工学院 Protein retention expansion microscopy
CN108474029B (en) 2015-08-07 2021-07-23 麻省理工学院 Nanoscale imaging of proteins and nucleic acids by extended microscopy
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
US20170241911A1 (en) 2016-02-22 2017-08-24 Miltenyi Biotec Gmbh Automated analysis tool for biological specimens
WO2017147483A1 (en) 2016-02-26 2017-08-31 The Board Of Trustees Of The Leland Stanford Junior University Multiplexed single molecule rna visualization with a two-probe proximity ligation system
US20170253918A1 (en) 2016-03-01 2017-09-07 Expansion Technologies Combining protein barcoding with expansion microscopy for in-situ, spatially-resolved proteomics
WO2017156336A1 (en) * 2016-03-10 2017-09-14 The Board Of Trustees Of The Leland Stanford Junior University Transposase-mediated imaging of the accessible genome
WO2017161251A1 (en) 2016-03-17 2017-09-21 President And Fellows Of Harvard College Methods for detecting and identifying genomic nucleic acids
US20180052081A1 (en) 2016-05-11 2018-02-22 Expansion Technologies Combining modified antibodies with expansion microscopy for in-situ, spatially-resolved proteomics
US10894990B2 (en) * 2016-05-17 2021-01-19 Shoreline Biome, Llc High throughput method for identification and sequencing of unknown microbial and eukaryotic genomes from complex mixtures
EP3472359B1 (en) 2016-06-21 2022-03-16 10X Genomics, Inc. Nucleic acid sequencing
JP7017554B2 (en) 2016-07-27 2022-02-08 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー Highly multiplexed fluorescence imaging
WO2018045186A1 (en) 2016-08-31 2018-03-08 President And Fellows Of Harvard College Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing
EP3507364A4 (en) 2016-08-31 2020-05-20 President and Fellows of Harvard College Methods of generating libraries of nucleic acid sequences for detection via fluorescent in situ sequencing
US11505819B2 (en) 2016-09-22 2022-11-22 William Marsh Rice University Molecular hybridization probes for complex sequence capture and analysis
SG11201903519UA (en) 2016-10-19 2019-05-30 10X Genomics Inc Methods and systems for barcoding nucleic acid molecules from individual cells or cell populations
GB201619458D0 (en) 2016-11-17 2017-01-04 Spatial Transcriptomics Ab Method for spatial tagging and analysing nucleic acids in a biological specimen
US10656144B2 (en) 2016-12-02 2020-05-19 The Charlotte Mecklenburg Hospital Authority Immune profiling and minimal residue disease following stem cell transplantation in multiple myeloma
KR20230166141A (en) 2016-12-09 2023-12-06 얼티뷰, 인크. Improved methods for multiplex imaging using labeled nucleic acid imaging agents
WO2019113533A1 (en) 2017-12-08 2019-06-13 10X Genomics, Inc. Methods and compositions for labeling cells
US10995361B2 (en) 2017-01-23 2021-05-04 Massachusetts Institute Of Technology Multiplexed signal amplified FISH via splinted ligation amplification and sequencing
CN111263819A (en) 2017-10-06 2020-06-09 卡特阿纳公司 RNA templated ligation
US11753676B2 (en) 2017-10-11 2023-09-12 Expansion Technologies Multiplexed in situ hybridization of tissue sections for spatially resolved transcriptomics with expansion microscopy

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