SG11202102003TA - Methods of generating an array - Google Patents
Methods of generating an arrayInfo
- Publication number
- SG11202102003TA SG11202102003TA SG11202102003TA SG11202102003TA SG11202102003TA SG 11202102003T A SG11202102003T A SG 11202102003TA SG 11202102003T A SG11202102003T A SG 11202102003TA SG 11202102003T A SG11202102003T A SG 11202102003TA SG 11202102003T A SG11202102003T A SG 11202102003TA
- Authority
- SG
- Singapore
- Prior art keywords
- array
- generating
- methods
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Lubricants (AREA)
- Compositions Of Macromolecular Compounds (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Applications Claiming Priority (43)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201862723950P | 2018-08-28 | 2018-08-28 | |
US201862723964P | 2018-08-28 | 2018-08-28 | |
US201862723957P | 2018-08-28 | 2018-08-28 | |
US201862723972P | 2018-08-28 | 2018-08-28 | |
US201862723960P | 2018-08-28 | 2018-08-28 | |
US201862723970P | 2018-08-28 | 2018-08-28 | |
US201862724487P | 2018-08-29 | 2018-08-29 | |
US201862724483P | 2018-08-29 | 2018-08-29 | |
US201862724561P | 2018-08-29 | 2018-08-29 | |
US201862724489P | 2018-08-29 | 2018-08-29 | |
US201862779342P | 2018-12-13 | 2018-12-13 | |
US201962788885P | 2019-01-06 | 2019-01-06 | |
US201962788905P | 2019-01-06 | 2019-01-06 | |
US201962788867P | 2019-01-06 | 2019-01-06 | |
US201962788871P | 2019-01-06 | 2019-01-06 | |
US201962788897P | 2019-01-06 | 2019-01-06 | |
US201962812219P | 2019-02-28 | 2019-02-28 | |
US201962819449P | 2019-03-15 | 2019-03-15 | |
US201962819496P | 2019-03-15 | 2019-03-15 | |
US201962819456P | 2019-03-15 | 2019-03-15 | |
US201962819448P | 2019-03-15 | 2019-03-15 | |
US201962819458P | 2019-03-15 | 2019-03-15 | |
US201962819468P | 2019-03-15 | 2019-03-15 | |
US201962819486P | 2019-03-15 | 2019-03-15 | |
US201962819478P | 2019-03-15 | 2019-03-15 | |
US201962819467P | 2019-03-15 | 2019-03-15 | |
US201962822592P | 2019-03-22 | 2019-03-22 | |
US201962822565P | 2019-03-22 | 2019-03-22 | |
US201962822554P | 2019-03-22 | 2019-03-22 | |
US201962822618P | 2019-03-22 | 2019-03-22 | |
US201962822627P | 2019-03-22 | 2019-03-22 | |
US201962822649P | 2019-03-22 | 2019-03-22 | |
US201962822605P | 2019-03-22 | 2019-03-22 | |
US201962822575P | 2019-03-22 | 2019-03-22 | |
US201962822632P | 2019-03-22 | 2019-03-22 | |
US201962839223P | 2019-04-26 | 2019-04-26 | |
US201962839346P | 2019-04-26 | 2019-04-26 | |
US201962839526P | 2019-04-26 | 2019-04-26 | |
US201962839320P | 2019-04-26 | 2019-04-26 | |
US201962842463P | 2019-05-02 | 2019-05-02 | |
US201962858331P | 2019-06-07 | 2019-06-07 | |
US201962860993P | 2019-06-13 | 2019-06-13 | |
PCT/US2019/048427 WO2020047004A2 (en) | 2018-08-28 | 2019-08-27 | Methods of generating an array |
Publications (1)
Publication Number | Publication Date |
---|---|
SG11202102003TA true SG11202102003TA (en) | 2021-03-30 |
Family
ID=69643282
Family Applications (4)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11202102029TA SG11202102029TA (en) | 2018-08-28 | 2019-08-27 | Methods for generating spatially barcoded arrays |
SG11202102019UA SG11202102019UA (en) | 2018-08-28 | 2019-08-27 | Method for transposase-mediated spatial tagging and analyzing genomic dna in a biological sample |
SG11202101944RA SG11202101944RA (en) | 2018-08-28 | 2019-08-27 | Increasing spatial array resolution |
SG11202102003TA SG11202102003TA (en) | 2018-08-28 | 2019-08-27 | Methods of generating an array |
Family Applications Before (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11202102029TA SG11202102029TA (en) | 2018-08-28 | 2019-08-27 | Methods for generating spatially barcoded arrays |
SG11202102019UA SG11202102019UA (en) | 2018-08-28 | 2019-08-27 | Method for transposase-mediated spatial tagging and analyzing genomic dna in a biological sample |
SG11202101944RA SG11202101944RA (en) | 2018-08-28 | 2019-08-27 | Increasing spatial array resolution |
Country Status (5)
Country | Link |
---|---|
US (5) | US20210332425A1 (en) |
EP (4) | EP3844306A2 (en) |
CN (3) | CN113286893A (en) |
SG (4) | SG11202102029TA (en) |
WO (4) | WO2020047007A2 (en) |
Families Citing this family (84)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
PT2556171E (en) | 2010-04-05 | 2015-12-21 | Prognosys Biosciences Inc | Spatially encoded biological assays |
US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
CN105849275B (en) | 2013-06-25 | 2020-03-17 | 普罗格诺西斯生物科学公司 | Method and system for detecting spatial distribution of biological targets in a sample |
EP3530752B1 (en) | 2015-04-10 | 2021-03-24 | Spatial Transcriptomics AB | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
EP3788171B1 (en) | 2018-05-03 | 2023-04-05 | Becton, Dickinson and Company | High throughput multiomics sample analysis |
US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
EP3894585A2 (en) | 2018-12-10 | 2021-10-20 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
US20240026445A1 (en) * | 2019-01-06 | 2024-01-25 | 10X Genomics, Inc. | Methods for determining a location of a biological analyte in a biological sample |
CN113631764A (en) * | 2019-02-21 | 2021-11-09 | 斯特拉托斯基因公司 | Methods, compositions, and apparatus for solid state synthesis of scalable polymers for use in single molecule sequencing |
EP3976820A1 (en) | 2019-05-30 | 2022-04-06 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
EP4025711A2 (en) | 2019-11-08 | 2022-07-13 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
WO2021091611A1 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Spatially-tagged analyte capture agents for analyte multiplexing |
WO2021097255A1 (en) * | 2019-11-13 | 2021-05-20 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
CN117746422A (en) * | 2019-11-22 | 2024-03-22 | 10X基因组学有限公司 | System and method for spatially analyzing analytes using fiducial alignment |
EP4073241A2 (en) | 2019-12-11 | 2022-10-19 | 10X Genomics, Inc. | Reverse transcriptase variants |
SG11202106899SA (en) | 2019-12-23 | 2021-09-29 | 10X Genomics Inc | Methods for spatial analysis using rna-templated ligation |
JP2023511279A (en) | 2020-01-13 | 2023-03-17 | フルーエント バイオサイエンシーズ インコーポレイテッド | Single cell sequencing |
US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
US11821035B1 (en) | 2020-01-29 | 2023-11-21 | 10X Genomics, Inc. | Compositions and methods of making gene expression libraries |
US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
US11835462B2 (en) | 2020-02-11 | 2023-12-05 | 10X Genomics, Inc. | Methods and compositions for partitioning a biological sample |
WO2021168278A1 (en) * | 2020-02-20 | 2021-08-26 | 10X Genomics, Inc. | METHODS TO COMBINE FIRST AND SECOND STRAND cDNA SYNTHESIS FOR SPATIAL ANALYSIS |
EP4107285A1 (en) | 2020-02-21 | 2022-12-28 | 10X Genomics, Inc. | Methods and compositions for integrated in situ spatial assay |
EP4107282A1 (en) * | 2020-02-21 | 2022-12-28 | 10X Genomics, Inc. | Capturing genetic targets using a hybridization approach |
US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
US11926863B1 (en) | 2020-02-27 | 2024-03-12 | 10X Genomics, Inc. | Solid state single cell method for analyzing fixed biological cells |
US11768175B1 (en) | 2020-03-04 | 2023-09-26 | 10X Genomics, Inc. | Electrophoretic methods for spatial analysis |
EP4121016A1 (en) | 2020-03-16 | 2023-01-25 | Fluent Biosciences Inc. | Multi-omic analysis in monodisperse droplets |
EP4136227A1 (en) | 2020-04-16 | 2023-02-22 | 10X Genomics, Inc. | Compositions and methods for use with fixed samples |
EP4242325A3 (en) | 2020-04-22 | 2023-10-04 | 10X Genomics, Inc. | Methods for spatial analysis using targeted rna depletion |
US20230265491A1 (en) * | 2020-05-04 | 2023-08-24 | 10X Genomics, Inc. | Spatial transcriptomic transfer modes |
US11851700B1 (en) | 2020-05-13 | 2023-12-26 | 10X Genomics, Inc. | Methods, kits, and compositions for processing extracellular molecules |
EP4153775A1 (en) | 2020-05-22 | 2023-03-29 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
EP4153776A1 (en) | 2020-05-22 | 2023-03-29 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
WO2021242834A1 (en) | 2020-05-26 | 2021-12-02 | 10X Genomics, Inc. | Method for resetting an array |
CN116249785A (en) | 2020-06-02 | 2023-06-09 | 10X基因组学有限公司 | Space transcriptomics for antigen-receptor |
EP4025692A2 (en) | 2020-06-02 | 2022-07-13 | 10X Genomics, Inc. | Nucleic acid library methods |
WO2021252499A1 (en) | 2020-06-08 | 2021-12-16 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
EP4165207A1 (en) | 2020-06-10 | 2023-04-19 | 10X Genomics, Inc. | Methods for determining a location of an analyte in a biological sample |
AU2021294334A1 (en) | 2020-06-25 | 2023-02-02 | 10X Genomics, Inc. | Spatial analysis of DNA methylation |
US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
US11981960B1 (en) | 2020-07-06 | 2024-05-14 | 10X Genomics, Inc. | Spatial analysis utilizing degradable hydrogels |
US11802312B2 (en) | 2020-08-10 | 2023-10-31 | Dimension Genomics Inc. | Devices and methods for multi-dimensional genome analysis |
US11981958B1 (en) | 2020-08-20 | 2024-05-14 | 10X Genomics, Inc. | Methods for spatial analysis using DNA capture |
EP4213993A2 (en) * | 2020-09-18 | 2023-07-26 | 10X Genomics, Inc. | Sample handling apparatus and fluid delivery methods |
US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
CN112143784A (en) * | 2020-09-29 | 2020-12-29 | 生物岛实验室 | Space omics sequencing, single-cell apparent transcriptomics sequencing and positioning identification method |
WO2022067565A1 (en) * | 2020-09-29 | 2022-04-07 | 生物岛实验室 | Spatial omics sequencing method, single cell epitranscriptomic sequencing method, and localization labeling method |
US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
WO2022132645A1 (en) * | 2020-12-14 | 2022-06-23 | Chan Zuckerberg Biohub, Inc. | Spatial genomics with single cell resolution |
AU2021409136A1 (en) | 2020-12-21 | 2023-06-29 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
US20220314187A1 (en) * | 2020-12-30 | 2022-10-06 | 10X Genomics, Inc. | Methods and compositions for light-controlled surface patterning using a polymer |
EP4271804A1 (en) * | 2020-12-30 | 2023-11-08 | Dovetail Genomics, LLC | Methods and compositions for sequencing library preparation |
WO2022147296A1 (en) | 2020-12-30 | 2022-07-07 | 10X Genomics, Inc. | Cleavage of capture probes for spatial analysis |
EP4271509A1 (en) * | 2020-12-30 | 2023-11-08 | 10X Genomics, Inc. | Molecular arrays and methods for generating and using the arrays |
US20240060122A1 (en) * | 2021-01-12 | 2024-02-22 | Paris Sciences Et Lettres | Method for spatial mapping and sequencing of cells or organelles |
WO2022198068A1 (en) | 2021-03-18 | 2022-09-22 | 10X Genomics, Inc. | Multiplex capture of gene and protein expression from a biological sample |
WO2022221425A1 (en) * | 2021-04-14 | 2022-10-20 | 10X Genomics, Inc. | Methods of measuring mislocalization of an analyte |
WO2022226057A1 (en) * | 2021-04-20 | 2022-10-27 | 10X Genomics, Inc. | Methods for assessing sample quality prior to spatial analysis using templated ligation |
WO2022226372A1 (en) * | 2021-04-23 | 2022-10-27 | Ultima Genomics, Inc. | Systems and methods for spatial reference sequencing |
WO2022232571A1 (en) | 2021-04-30 | 2022-11-03 | 10X Genomics, Inc. | Fusion rt variants for improved performance |
WO2022251419A1 (en) * | 2021-05-26 | 2022-12-01 | Board Of Regents, The University Of Texas System | Methods and systems for single cell protein analysis |
WO2022251445A1 (en) * | 2021-05-28 | 2022-12-01 | Fluent Biosciences Inc. | Spatial transcriptomics in pips |
EP4347879A1 (en) * | 2021-06-03 | 2024-04-10 | 10X Genomics, Inc. | Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis |
WO2022265965A1 (en) | 2021-06-14 | 2022-12-22 | 10X Genomics, Inc. | Reverse transcriptase variants for improved performance |
WO2023034489A1 (en) | 2021-09-01 | 2023-03-09 | 10X Genomics, Inc. | Methods, compositions, and kits for blocking a capture probe on a spatial array |
WO2023059658A1 (en) * | 2021-10-04 | 2023-04-13 | AtlasXomics Inc. | System and methods for high throughput screening of tissue preparation conditions |
WO2023076345A1 (en) * | 2021-10-26 | 2023-05-04 | 10X Genomics, Inc. | Methods for spatial analysis using targeted rna capture |
WO2023114473A2 (en) | 2021-12-16 | 2023-06-22 | 10X Genomics, Inc. | Recombinant reverse transcriptase variants for improved performance |
WO2023116938A1 (en) * | 2021-12-24 | 2023-06-29 | 映泰科技有限公司 | Biochip for spatial transcriptomic analysis, and preparation method and application thereof |
CN116694730A (en) * | 2022-02-28 | 2023-09-05 | 南方科技大学 | Construction method of single cell open chromatin and transcriptome co-sequencing library |
CN114574569B (en) * | 2022-03-28 | 2023-05-16 | 浙江大学 | Genome sequencing kit and sequencing method based on terminal transferase |
WO2023196526A1 (en) | 2022-04-06 | 2023-10-12 | 10X Genomics, Inc. | Methods for multiplex cell analysis |
WO2023215612A1 (en) | 2022-05-06 | 2023-11-09 | 10X Genomics, Inc. | Analysis of antigen and antigen receptor interactions |
WO2023225519A1 (en) | 2022-05-17 | 2023-11-23 | 10X Genomics, Inc. | Modified transposons, compositions and uses thereof |
WO2023250077A1 (en) * | 2022-06-22 | 2023-12-28 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
WO2024006799A1 (en) * | 2022-06-29 | 2024-01-04 | 10X Genomics, Inc. | Covalent attachment of splint oligonucleotides for molecular array generation using ligation |
WO2024006832A1 (en) * | 2022-06-29 | 2024-01-04 | 10X Genomics, Inc. | Click chemistry-based dna photo-ligation for manufacturing of high-resolution dna arrays |
Family Cites Families (135)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
US4968601A (en) * | 1988-02-09 | 1990-11-06 | The United States Of America As Represented By The Dept. Of Health & Human Services | Method for diagnosing latent viral infection |
US5472881A (en) | 1992-11-12 | 1995-12-05 | University Of Utah Research Foundation | Thiol labeling of DNA for attachment to gold surfaces |
DE69434520T3 (en) | 1993-07-30 | 2009-10-15 | Affymax, Inc., Palo Alto | BIOTINYLATION OF PROTEINS |
US5610287A (en) | 1993-12-06 | 1997-03-11 | Molecular Tool, Inc. | Method for immobilizing nucleic acid molecules |
US5512462A (en) | 1994-02-25 | 1996-04-30 | Hoffmann-La Roche Inc. | Methods and reagents for the polymerase chain reaction amplification of long DNA sequences |
US5677170A (en) | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
US5552278A (en) | 1994-04-04 | 1996-09-03 | Spectragen, Inc. | DNA sequencing by stepwise ligation and cleavage |
US5807522A (en) | 1994-06-17 | 1998-09-15 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for fabricating microarrays of biological samples |
US5641658A (en) | 1994-08-03 | 1997-06-24 | Mosaic Technologies, Inc. | Method for performing amplification of nucleic acid with two primers bound to a single solid support |
US5846719A (en) | 1994-10-13 | 1998-12-08 | Lynx Therapeutics, Inc. | Oligonucleotide tags for sorting and identification |
US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
US5925545A (en) | 1996-09-09 | 1999-07-20 | Wisconsin Alumni Research Foundation | System for in vitro transposition |
US5965443A (en) | 1996-09-09 | 1999-10-12 | Wisconsin Alumni Research Foundation | System for in vitro transposition |
GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
GB9626815D0 (en) | 1996-12-23 | 1997-02-12 | Cemu Bioteknik Ab | Method of sequencing DNA |
EP0968223B1 (en) | 1997-01-08 | 2016-12-21 | Sigma-Aldrich Co. LLC | Bioconjugation of macromolecules |
US5837860A (en) | 1997-03-05 | 1998-11-17 | Molecular Tool, Inc. | Covalent attachment of nucleic acid molecules onto solid-phases via disulfide bonds |
US6327410B1 (en) | 1997-03-14 | 2001-12-04 | The Trustees Of Tufts College | Target analyte sensors utilizing Microspheres |
US6023540A (en) | 1997-03-14 | 2000-02-08 | Trustees Of Tufts College | Fiber optic sensor with encoded microspheres |
ATE269908T1 (en) | 1997-04-01 | 2004-07-15 | Manteia S A | METHOD FOR SEQUENCING NUCLEIC ACIDS |
AU6846698A (en) | 1997-04-01 | 1998-10-22 | Glaxo Group Limited | Method of nucleic acid amplification |
US6969488B2 (en) | 1998-05-22 | 2005-11-29 | Solexa, Inc. | System and apparatus for sequential processing of analytes |
US5919626A (en) | 1997-06-06 | 1999-07-06 | Orchid Bio Computer, Inc. | Attachment of unmodified nucleic acids to silanized solid phase surfaces |
US7427678B2 (en) | 1998-01-08 | 2008-09-23 | Sigma-Aldrich Co. | Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method |
US6387632B2 (en) * | 1998-06-11 | 2002-05-14 | Hitachi, Ltd. | Polynucleotide separation method and apparatus therefor |
EP2045334A1 (en) | 1998-06-24 | 2009-04-08 | Illumina, Inc. | Decoding of array sensors with microspheres |
US6159736A (en) | 1998-09-23 | 2000-12-12 | Wisconsin Alumni Research Foundation | Method for making insertional mutations using a Tn5 synaptic complex |
AR021833A1 (en) | 1998-09-30 | 2002-08-07 | Applied Research Systems | METHODS OF AMPLIFICATION AND SEQUENCING OF NUCLEIC ACID |
US6391937B1 (en) | 1998-11-25 | 2002-05-21 | Motorola, Inc. | Polyacrylamide hydrogels and hydrogel arrays made from polyacrylamide reactive prepolymers |
US6355431B1 (en) | 1999-04-20 | 2002-03-12 | Illumina, Inc. | Detection of nucleic acid amplification reactions using bead arrays |
ATE413467T1 (en) | 1999-04-20 | 2008-11-15 | Illumina Inc | DETECTION OF NUCLEIC ACID REACTIONS ON BEAD ARRAYS |
US20060275782A1 (en) * | 1999-04-20 | 2006-12-07 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
WO2001009363A1 (en) | 1999-08-02 | 2001-02-08 | Wisconsin Alumni Research Foundation | Mutant tn5 transposase enzymes and method for their use |
US7244559B2 (en) | 1999-09-16 | 2007-07-17 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
US6274320B1 (en) | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
US6770441B2 (en) | 2000-02-10 | 2004-08-03 | Illumina, Inc. | Array compositions and methods of making same |
US7001792B2 (en) | 2000-04-24 | 2006-02-21 | Eagle Research & Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
GB0018120D0 (en) | 2000-07-24 | 2000-09-13 | Fermentas Ab | Nuclease |
AR031640A1 (en) | 2000-12-08 | 2003-09-24 | Applied Research Systems | ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS IN A SOLID SUPPORT |
US7297778B2 (en) * | 2001-07-25 | 2007-11-20 | Affymetrix, Inc. | Complexity management of genomic DNA |
US20040019005A1 (en) * | 2001-10-01 | 2004-01-29 | Jeffrey Van Ness | Methods for parallel measurement of genetic variations |
US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
WO2003070294A2 (en) | 2002-01-16 | 2003-08-28 | Mayo Foundation For Medical Education And Research | Method and apparatus for image-guided therapy |
US20040002090A1 (en) | 2002-03-05 | 2004-01-01 | Pascal Mayer | Methods for detecting genome-wide sequence variations associated with a phenotype |
KR101138643B1 (en) | 2002-05-30 | 2012-04-26 | 더 스크립스 리서치 인스티튜트 | Copper-catalysed ligation of azides and acetylenes |
SI3002289T1 (en) | 2002-08-23 | 2018-07-31 | Illumina Cambridge Limited | Modified nucleotides for polynucleotide sequencing |
KR20040062847A (en) * | 2003-01-03 | 2004-07-09 | 삼성전자주식회사 | Method for replicating nucleic acid array |
GB0302058D0 (en) * | 2003-01-29 | 2003-02-26 | Univ Cranfield | Replication of nucleic acid arrays |
US7083980B2 (en) | 2003-04-17 | 2006-08-01 | Wisconsin Alumni Research Foundation | Tn5 transposase mutants and the use thereof |
US20050031176A1 (en) | 2003-08-08 | 2005-02-10 | Hertel Sarah R. | Method and apparatus of multi-modality image fusion |
GB0321306D0 (en) | 2003-09-11 | 2003-10-15 | Solexa Ltd | Modified polymerases for improved incorporation of nucleotide analogues |
US7259258B2 (en) | 2003-12-17 | 2007-08-21 | Illumina, Inc. | Methods of attaching biological compounds to solid supports using triazine |
JP2007525571A (en) | 2004-01-07 | 2007-09-06 | ソレクサ リミテッド | Modified molecular array |
US7608434B2 (en) | 2004-08-04 | 2009-10-27 | Wisconsin Alumni Research Foundation | Mutated Tn5 transposase proteins and the use thereof |
GB0427236D0 (en) | 2004-12-13 | 2005-01-12 | Solexa Ltd | Improved method of nucleotide detection |
WO2006064199A1 (en) | 2004-12-13 | 2006-06-22 | Solexa Limited | Improved method of nucleotide detection |
CN109485727A (en) * | 2005-05-09 | 2019-03-19 | 小野药品工业株式会社 | The human monoclonal antibodies of programmed death-1 (PD-1) and the method for carrying out treating cancer using anti-PD-1 antibody |
EP1888743B1 (en) | 2005-05-10 | 2011-08-03 | Illumina Cambridge Limited | Improved polymerases |
ES2565670T3 (en) | 2005-06-20 | 2016-04-06 | Advanced Cell Diagnostics, Inc. | Nucleic acid detection methods in individual cells and identification of rare cells in large populations of heterogeneous cells |
GB0514936D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Preparation of templates for nucleic acid sequencing |
GB0522310D0 (en) | 2005-11-01 | 2005-12-07 | Solexa Ltd | Methods of preparing libraries of template polynucleotides |
EP2021503A1 (en) | 2006-03-17 | 2009-02-11 | Solexa Ltd. | Isothermal methods for creating clonal single molecule arrays |
US20070231823A1 (en) * | 2006-03-23 | 2007-10-04 | Mckernan Kevin J | Directed enrichment of genomic DNA for high-throughput sequencing |
EP3536396B1 (en) | 2006-08-07 | 2022-03-30 | The President and Fellows of Harvard College | Fluorocarbon emulsion stabilizing surfactants |
WO2008022332A2 (en) * | 2006-08-18 | 2008-02-21 | Board Of Regents, The University Of Texas System | System, method and kit for replicating a dna array |
ES2923759T3 (en) | 2006-12-14 | 2022-09-30 | Life Technologies Corp | Apparatus for measuring analytes using FET arrays |
US8262900B2 (en) | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
US20100055733A1 (en) | 2008-09-04 | 2010-03-04 | Lutolf Matthias P | Manufacture and uses of reactive microcontact printing of biomolecules on soft hydrogels |
US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
ES2719502T3 (en) | 2010-01-29 | 2019-07-10 | Advanced Cell Diagnostics Inc | Nucleic acid in situ detection methods |
EP2354242A1 (en) * | 2010-02-03 | 2011-08-10 | Epiontis GmbH | Assay for determining the type and/or status of a cell based on the epigenetic pattern and the chromatin structure |
PT2556171E (en) | 2010-04-05 | 2015-12-21 | Prognosys Biosciences Inc | Spatially encoded biological assays |
US8951781B2 (en) | 2011-01-10 | 2015-02-10 | Illumina, Inc. | Systems, methods, and apparatuses to image a sample for biological or chemical analysis |
GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
EP2739752B1 (en) * | 2011-08-03 | 2017-07-19 | Bio-Rad Laboratories, Inc. | Filtering small nucleic acids using permeabilized cells |
DK3623481T3 (en) | 2011-09-23 | 2021-11-15 | Illumina Inc | COMPOSITIONS FOR NUCLEIC ACID SEQUENCE |
WO2013096838A2 (en) * | 2011-12-22 | 2013-06-27 | Ibis Biosciences, Inc. | Systems and methods for isolating nucleic acids |
US9193996B2 (en) | 2012-04-03 | 2015-11-24 | Illumina, Inc. | Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing |
US9012022B2 (en) | 2012-06-08 | 2015-04-21 | Illumina, Inc. | Polymer coatings |
US8895249B2 (en) | 2012-06-15 | 2014-11-25 | Illumina, Inc. | Kinetic exclusion amplification of nucleic acid libraries |
CA2881685C (en) | 2012-08-14 | 2023-12-05 | 10X Genomics, Inc. | Microcapsule compositions and methods |
US20140378345A1 (en) | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
US9783841B2 (en) | 2012-10-04 | 2017-10-10 | The Board Of Trustees Of The Leland Stanford Junior University | Detection of target nucleic acids in a cellular sample |
CA2886974C (en) | 2012-10-17 | 2021-06-29 | Spatial Transcriptomics Ab | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
WO2014085725A1 (en) | 2012-11-27 | 2014-06-05 | Tufts University | Biopolymer-based inks and use thereof |
US9512422B2 (en) | 2013-02-26 | 2016-12-06 | Illumina, Inc. | Gel patterned surfaces |
EP3578666A1 (en) | 2013-03-12 | 2019-12-11 | President and Fellows of Harvard College | Method of generating a three-dimensional nucleic acid containing matrix |
WO2014149629A1 (en) * | 2013-03-15 | 2014-09-25 | Htg Molecular Diagnostics, Inc. | Subtyping lung cancers |
CN105849275B (en) | 2013-06-25 | 2020-03-17 | 普罗格诺西斯生物科学公司 | Method and system for detecting spatial distribution of biological targets in a sample |
US20150000854A1 (en) | 2013-06-27 | 2015-01-01 | The Procter & Gamble Company | Sheet products bearing designs that vary among successive sheets, and apparatus and methods for producing the same |
CN105658310A (en) | 2013-09-13 | 2016-06-08 | 斯坦福大学托管董事会 | Multiplexed imaging of tissues using mass tags and secondary ion mass spectrometry |
WO2015094123A1 (en) * | 2013-12-20 | 2015-06-25 | National University Of Singapore | Differentiation therapy with cd137 ligand agonists |
CN106460069B (en) | 2014-04-18 | 2021-02-12 | 威廉马歇莱思大学 | Competitive compositions for enriching nucleic acid molecules for rare allele-containing material |
CA2951325C (en) | 2014-06-06 | 2023-09-12 | Herlev Hospital | Determining antigen recognition through barcoding of mhc multimers |
US11585806B2 (en) | 2014-06-13 | 2023-02-21 | Immudex Aps | General detection and isolation of specific cells by binding of labeled molecules |
JP6838969B2 (en) | 2014-06-26 | 2021-03-03 | 10エックス ジェノミクス, インコーポレイテッド | Method for Analyzing Nucleic Acids Derived from Individual Cells or Cell Populations |
WO2016007839A1 (en) | 2014-07-11 | 2016-01-14 | President And Fellows Of Harvard College | Methods for high-throughput labelling and detection of biological features in situ using microscopy |
KR20170064540A (en) * | 2014-09-26 | 2017-06-09 | 투 포어 가이즈, 인코포레이티드 | Target sequence detection by nanopore sensing of synthetic probes |
US20160108458A1 (en) | 2014-10-06 | 2016-04-21 | The Board Of Trustees Of The Leland Stanford Junior University | Multiplexed detection and quantification of nucleic acids in single-cells |
ES2836802T3 (en) | 2015-02-27 | 2021-06-28 | Becton Dickinson Co | Spatially addressable molecular barcodes |
ES2934982T3 (en) * | 2015-03-30 | 2023-02-28 | Becton Dickinson Co | Methods for encoding with combinatorial barcodes |
EP3530752B1 (en) * | 2015-04-10 | 2021-03-24 | Spatial Transcriptomics AB | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
US10059990B2 (en) | 2015-04-14 | 2018-08-28 | Massachusetts Institute Of Technology | In situ nucleic acid sequencing of expanded biological samples |
CN107709574B (en) | 2015-04-14 | 2021-10-01 | 皇家飞利浦有限公司 | Spatial mapping of molecular profiles of biological tissue samples |
JP6971218B2 (en) | 2015-07-17 | 2021-11-24 | ナノストリング テクノロジーズ,インコーポレイティド | Simultaneous quantification of gene expression in user-defined regions of cross-tissue sections |
CA3176469A1 (en) | 2015-07-27 | 2017-02-02 | Illumina, Inc. | Spatial mapping of nucleic acid sequence information |
CN108139408B (en) | 2015-08-07 | 2020-08-28 | 麻省理工学院 | Protein retention expansion microscopy |
CN108474029B (en) | 2015-08-07 | 2021-07-23 | 麻省理工学院 | Nanoscale imaging of proteins and nucleic acids by extended microscopy |
SG11201804086VA (en) | 2015-12-04 | 2018-06-28 | 10X Genomics Inc | Methods and compositions for nucleic acid analysis |
US20170241911A1 (en) | 2016-02-22 | 2017-08-24 | Miltenyi Biotec Gmbh | Automated analysis tool for biological specimens |
WO2017147483A1 (en) | 2016-02-26 | 2017-08-31 | The Board Of Trustees Of The Leland Stanford Junior University | Multiplexed single molecule rna visualization with a two-probe proximity ligation system |
US20170253918A1 (en) | 2016-03-01 | 2017-09-07 | Expansion Technologies | Combining protein barcoding with expansion microscopy for in-situ, spatially-resolved proteomics |
WO2017156336A1 (en) * | 2016-03-10 | 2017-09-14 | The Board Of Trustees Of The Leland Stanford Junior University | Transposase-mediated imaging of the accessible genome |
WO2017161251A1 (en) | 2016-03-17 | 2017-09-21 | President And Fellows Of Harvard College | Methods for detecting and identifying genomic nucleic acids |
US20180052081A1 (en) | 2016-05-11 | 2018-02-22 | Expansion Technologies | Combining modified antibodies with expansion microscopy for in-situ, spatially-resolved proteomics |
US10894990B2 (en) * | 2016-05-17 | 2021-01-19 | Shoreline Biome, Llc | High throughput method for identification and sequencing of unknown microbial and eukaryotic genomes from complex mixtures |
EP3472359B1 (en) | 2016-06-21 | 2022-03-16 | 10X Genomics, Inc. | Nucleic acid sequencing |
JP7017554B2 (en) | 2016-07-27 | 2022-02-08 | ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー | Highly multiplexed fluorescence imaging |
WO2018045186A1 (en) | 2016-08-31 | 2018-03-08 | President And Fellows Of Harvard College | Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing |
EP3507364A4 (en) | 2016-08-31 | 2020-05-20 | President and Fellows of Harvard College | Methods of generating libraries of nucleic acid sequences for detection via fluorescent in situ sequencing |
US11505819B2 (en) | 2016-09-22 | 2022-11-22 | William Marsh Rice University | Molecular hybridization probes for complex sequence capture and analysis |
SG11201903519UA (en) | 2016-10-19 | 2019-05-30 | 10X Genomics Inc | Methods and systems for barcoding nucleic acid molecules from individual cells or cell populations |
GB201619458D0 (en) | 2016-11-17 | 2017-01-04 | Spatial Transcriptomics Ab | Method for spatial tagging and analysing nucleic acids in a biological specimen |
US10656144B2 (en) | 2016-12-02 | 2020-05-19 | The Charlotte Mecklenburg Hospital Authority | Immune profiling and minimal residue disease following stem cell transplantation in multiple myeloma |
KR20230166141A (en) | 2016-12-09 | 2023-12-06 | 얼티뷰, 인크. | Improved methods for multiplex imaging using labeled nucleic acid imaging agents |
WO2019113533A1 (en) | 2017-12-08 | 2019-06-13 | 10X Genomics, Inc. | Methods and compositions for labeling cells |
US10995361B2 (en) | 2017-01-23 | 2021-05-04 | Massachusetts Institute Of Technology | Multiplexed signal amplified FISH via splinted ligation amplification and sequencing |
CN111263819A (en) | 2017-10-06 | 2020-06-09 | 卡特阿纳公司 | RNA templated ligation |
US11753676B2 (en) | 2017-10-11 | 2023-09-12 | Expansion Technologies | Multiplexed in situ hybridization of tissue sections for spatially resolved transcriptomics with expansion microscopy |
-
2019
- 2019-08-27 EP EP19773224.1A patent/EP3844306A2/en active Pending
- 2019-08-27 US US17/271,995 patent/US20210332425A1/en active Pending
- 2019-08-27 CN CN201980071234.6A patent/CN113286893A/en active Pending
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- 2019-08-27 EP EP19765928.7A patent/EP3844304A2/en active Pending
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- 2019-08-27 SG SG11202102019UA patent/SG11202102019UA/en unknown
- 2019-08-27 US US18/023,257 patent/US20230323447A1/en active Pending
- 2019-08-27 EP EP19773221.7A patent/EP3844307A1/en active Pending
- 2019-08-27 WO PCT/US2019/048430 patent/WO2020047007A2/en unknown
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- 2019-08-27 SG SG11202102003TA patent/SG11202102003TA/en unknown
- 2019-08-27 WO PCT/US2019/048425 patent/WO2020047002A1/en unknown
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- 2019-08-27 WO PCT/US2019/048434 patent/WO2020047010A2/en unknown
- 2019-08-27 WO PCT/US2019/048427 patent/WO2020047004A2/en unknown
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2020
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- 2020-05-18 US US16/876,682 patent/US20200407781A1/en not_active Abandoned
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US20210332424A1 (en) | 2021-10-28 |
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WO2020047007A2 (en) | 2020-03-05 |
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US20210332425A1 (en) | 2021-10-28 |
WO2020047010A3 (en) | 2020-04-16 |
EP3844304A2 (en) | 2021-07-07 |
WO2020047007A3 (en) | 2020-04-16 |
SG11202102019UA (en) | 2021-03-30 |
US20210010070A1 (en) | 2021-01-14 |
EP3844305A2 (en) | 2021-07-07 |
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