SG11202100756RA - Cell culture strategies for modulating protein glycosylation - Google Patents

Cell culture strategies for modulating protein glycosylation

Info

Publication number
SG11202100756RA
SG11202100756RA SG11202100756RA SG11202100756RA SG11202100756RA SG 11202100756R A SG11202100756R A SG 11202100756RA SG 11202100756R A SG11202100756R A SG 11202100756RA SG 11202100756R A SG11202100756R A SG 11202100756RA SG 11202100756R A SG11202100756R A SG 11202100756RA
Authority
SG
Singapore
Prior art keywords
cell culture
protein glycosylation
modulating protein
culture strategies
strategies
Prior art date
Application number
SG11202100756RA
Inventor
Inn Yuk
Patrick Ahyow
Dang Anh Nguyen
Melissa Mun
Kristen Leigh Douglas
Robert David Kiss
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of SG11202100756RA publication Critical patent/SG11202100756RA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/005Glycopeptides, glycoproteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/10Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14Specific host cells or culture conditions, e.g. components, pH or temperature
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/40Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41Glycosylation, sialylation, or fucosylation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/02Atmosphere, e.g. low oxygen conditions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00Culture process characterised by temperature
SG11202100756RA 2018-08-10 2019-08-09 Cell culture strategies for modulating protein glycosylation SG11202100756RA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862717751P 2018-08-10 2018-08-10
PCT/US2019/045900 WO2020033827A1 (en) 2018-08-10 2019-08-09 Cell culture strategies for modulating protein glycosylation

Publications (1)

Publication Number Publication Date
SG11202100756RA true SG11202100756RA (en) 2021-02-25

Family

ID=67902575

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202100756RA SG11202100756RA (en) 2018-08-10 2019-08-09 Cell culture strategies for modulating protein glycosylation

Country Status (14)

Country Link
US (1) US20210238644A1 (en)
EP (1) EP3833688A1 (en)
JP (1) JP2021533760A (en)
KR (1) KR20210043618A (en)
CN (1) CN112752769A (en)
AR (1) AR114548A1 (en)
AU (1) AU2019319970A1 (en)
BR (1) BR112021002480A2 (en)
CA (1) CA3107038A1 (en)
IL (1) IL280553A (en)
MX (1) MX2021001521A (en)
SG (1) SG11202100756RA (en)
TW (1) TW202021985A (en)
WO (1) WO2020033827A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4352094A1 (en) 2021-06-07 2024-04-17 Amgen Inc. Using fucosidase to control afucosylation level of glycosylated proteins
CN115651953A (en) * 2022-11-17 2023-01-31 康日百奥生物科技(苏州)有限公司 Antibody medicine, preparation method and cell culture medium

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL85035A0 (en) 1987-01-08 1988-06-30 Int Genetic Eng Polynucleotide molecule,a chimeric antibody with specificity for human b cell surface antigen,a process for the preparation and methods utilizing the same
ES2206447T3 (en) 1991-06-14 2004-05-16 Genentech, Inc. HUMANIZED ANTIBODY FOR HEREGULINE.
ATE375365T1 (en) 1998-04-02 2007-10-15 Genentech Inc ANTIBODIES VARIANTS AND FRAGMENTS THEREOF
US6194551B1 (en) 1998-04-02 2001-02-27 Genentech, Inc. Polypeptide variants
AU2005233387B2 (en) * 2004-04-15 2011-05-26 Glycofi, Inc. Production of galactosylated glycoproteins in lower eukaryotes
JP5058483B2 (en) * 2005-09-14 2012-10-24 オリンパス株式会社 Long-term or continuous detection method for biological samples
BRPI1015019A2 (en) * 2009-04-20 2018-02-14 Pfizer glycosylation control of protein and related compositions and methods.
US20110053223A1 (en) * 2009-08-14 2011-03-03 Robert Bayer Cell culture methods to make antibodies with enhanced adcc function
EP2511293A1 (en) * 2011-04-13 2012-10-17 LEK Pharmaceuticals d.d. A method for controlling the main complex N-glycan structures and the acidic variants and variability in bioprocesses producing recombinant proteins
EP2702077A2 (en) * 2011-04-27 2014-03-05 AbbVie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
US9493744B2 (en) 2012-06-20 2016-11-15 Genentech, Inc. Methods for viral inactivation and other adventitious agents
WO2014170866A2 (en) * 2013-04-18 2014-10-23 Dr. Reddy's Laboratories Limited Process of obtaining glycoprotein composition with increased galactosylation content
EP3024922A4 (en) * 2013-07-23 2017-03-29 Biocon Limited Methods for controlling fucosylation levels in proteins
HU231463B1 (en) * 2015-08-04 2024-01-28 Richter Gedeon Nyrt. Method for increasing the galactose content of recombinant proteins

Also Published As

Publication number Publication date
KR20210043618A (en) 2021-04-21
US20210238644A1 (en) 2021-08-05
AR114548A1 (en) 2020-09-16
AU2019319970A1 (en) 2021-03-11
MX2021001521A (en) 2021-04-19
CN112752769A (en) 2021-05-04
JP2021533760A (en) 2021-12-09
TW202021985A (en) 2020-06-16
EP3833688A1 (en) 2021-06-16
BR112021002480A2 (en) 2021-07-27
CA3107038A1 (en) 2020-02-13
WO2020033827A1 (en) 2020-02-13
IL280553A (en) 2021-03-25

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