SG11201912236YA - Crispr reporter non-human animals and uses thereof - Google Patents
Crispr reporter non-human animals and uses thereofInfo
- Publication number
- SG11201912236YA SG11201912236YA SG11201912236YA SG11201912236YA SG11201912236YA SG 11201912236Y A SG11201912236Y A SG 11201912236YA SG 11201912236Y A SG11201912236Y A SG 11201912236YA SG 11201912236Y A SG11201912236Y A SG 11201912236YA SG 11201912236Y A SG11201912236Y A SG 11201912236YA
- Authority
- SG
- Singapore
- Prior art keywords
- human animals
- crispr reporter
- reporter non
- crispr
- animals
- Prior art date
Links
- 108091033409 CRISPR Proteins 0.000 title 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
- C12N15/861—Adenoviral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
- C12N15/877—Techniques for producing new mammalian cloned embryos
- C12N15/8775—Murine embryos
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
- C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/07—Animals genetically altered by homologous recombination
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/20—Animal model comprising regulated expression system
- A01K2217/206—Animal model comprising tissue-specific expression system, e.g. tissue specific expression of transgene, of Cre recombinase
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/105—Murine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/03—Animal model, e.g. for test or diseases
- A01K2267/0393—Animal model comprising a reporter system for screening tests
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/10—Applications; Uses in screening processes
- C12N2320/11—Applications; Uses in screening processes for the determination of target sites, i.e. of active nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Environmental Sciences (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biodiversity & Conservation Biology (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Medicinal Chemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Virology (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201762539279P | 2017-07-31 | 2017-07-31 | |
PCT/US2018/044606 WO2019028023A2 (en) | 2017-07-31 | 2018-07-31 | Methods and compositions for assessing crispr/cas-mediated disruption or excision and crispr/cas-induced recombination with an exogenous donor nucleic acid in vivo |
Publications (1)
Publication Number | Publication Date |
---|---|
SG11201912236YA true SG11201912236YA (en) | 2020-01-30 |
Family
ID=63524355
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11201912236YA SG11201912236YA (en) | 2017-07-31 | 2018-07-31 | Crispr reporter non-human animals and uses thereof |
Country Status (12)
Country | Link |
---|---|
US (2) | US11021719B2 (en) |
EP (1) | EP3585160A2 (en) |
JP (1) | JP2020533957A (en) |
KR (1) | KR20200033259A (en) |
CN (1) | CN111182790A (en) |
AU (1) | AU2018309708A1 (en) |
BR (1) | BR112020001996A2 (en) |
CA (1) | CA3068072A1 (en) |
IL (1) | IL272333A (en) |
MX (1) | MX2020001177A (en) |
SG (1) | SG11201912236YA (en) |
WO (1) | WO2019028023A2 (en) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SG11201912236YA (en) | 2017-07-31 | 2020-01-30 | Regeneron Pharma | Crispr reporter non-human animals and uses thereof |
GB2589246A (en) * | 2018-05-16 | 2021-05-26 | Synthego Corp | Methods and systems for guide RNA design and use |
BR112021007229A2 (en) | 2018-10-16 | 2021-08-10 | Blueallele, Llc | methods for targeted insertion of DNA into genes |
CN109266680B (en) * | 2018-10-17 | 2020-09-25 | 江苏集萃药康生物科技有限公司 | Method for preparing CKO/KI animal model by using Cas9 technology |
SG11202108090XA (en) * | 2019-03-18 | 2021-08-30 | Regeneron Pharma | Crispr/cas dropout screening platform to reveal genetic vulnerabilities associated with tau aggregation |
KR102264829B1 (en) | 2019-11-22 | 2021-06-14 | 한국원자력의학원 | Reporter System for Assessing Cleavage Activity of CRISPR/Cas9 |
CN112266931B (en) * | 2020-10-15 | 2022-04-29 | 海湃泰克(北京)生物医药科技有限公司 | Construction method and application of transgenic rat specifically expressing hMRGPRX4 |
WO2023064798A2 (en) * | 2021-10-13 | 2023-04-20 | The Board Of Trustees Of The Leland Stanford Junior University | Differential proliferation of human hematopoietic stem and progenitor cells using truncated erythropoietin receptors |
CN114703174B (en) * | 2022-04-12 | 2023-10-24 | 中国科学院海洋研究所 | CRISPR/Cas9 gene knockout method for rapidly obtaining genotype and phenotype mutation and application thereof |
CN114703231B (en) * | 2022-04-12 | 2023-10-24 | 中国科学院海洋研究所 | Electroporation gene editing method and application of crassostrea gigas beta-tubulin gene |
Family Cites Families (103)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU4220093A (en) | 1992-04-27 | 1993-11-29 | Indiana University Foundation | Live animal mutagenesis systems for testing mutagenic agents in vivo |
US5523226A (en) | 1993-05-14 | 1996-06-04 | Biotechnology Research And Development Corp. | Transgenic swine compositions and methods |
AU8587598A (en) | 1997-07-26 | 1999-02-16 | Wisconsin Alumni Research Foundation | Trans-species nuclear transfer |
US20050144655A1 (en) | 2000-10-31 | 2005-06-30 | Economides Aris N. | Methods of modifying eukaryotic cells |
US6586251B2 (en) | 2000-10-31 | 2003-07-01 | Regeneron Pharmaceuticals, Inc. | Methods of modifying eukaryotic cells |
AUPR451401A0 (en) | 2001-04-20 | 2001-05-24 | Monash University | A method of nuclear transfer |
CA2474486C (en) | 2002-01-23 | 2013-05-14 | The University Of Utah Research Foundation | Targeted chromosomal mutagenesis using zinc finger nucleases |
AU2003218382B2 (en) | 2002-03-21 | 2007-12-13 | Sangamo Therapeutics, Inc. | Methods and compositions for using zinc finger endonucleases to enhance homologous recombination |
US7612250B2 (en) | 2002-07-29 | 2009-11-03 | Trustees Of Tufts College | Nuclear transfer embryo formation method |
AU2003298574B2 (en) | 2002-09-05 | 2008-04-24 | California Institute Of Technology | Use of chimeric nucleases to stimulate gene targeting |
US8409861B2 (en) | 2003-08-08 | 2013-04-02 | Sangamo Biosciences, Inc. | Targeted deletion of cellular DNA sequences |
US7888121B2 (en) | 2003-08-08 | 2011-02-15 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
US7972854B2 (en) | 2004-02-05 | 2011-07-05 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
US20060063231A1 (en) | 2004-09-16 | 2006-03-23 | Sangamo Biosciences, Inc. | Compositions and methods for protein production |
EP2767161B1 (en) | 2004-10-19 | 2018-02-07 | Regeneron Pharmaceuticals, Inc. | Method for generating an non-human animal homozygous for a genetic modification |
EP2447279B1 (en) | 2006-05-25 | 2014-04-09 | Sangamo BioSciences, Inc. | Methods and compositions for gene inactivation |
JP5266210B2 (en) | 2006-05-25 | 2013-08-21 | サンガモ バイオサイエンシズ インコーポレイテッド | Modified cleavage half-domain |
JP4692417B2 (en) | 2006-06-30 | 2011-06-01 | 富士ゼロックス株式会社 | Image forming apparatus |
CN101117633B (en) | 2006-08-03 | 2011-07-20 | 上海交通大学附属儿童医院 | Nucleus transplantation method |
ATE489465T1 (en) * | 2007-04-26 | 2010-12-15 | Sangamo Biosciences Inc | TARGETED INTEGRATION INTO THE PPP1R12C POSITION |
WO2008149176A1 (en) | 2007-06-06 | 2008-12-11 | Cellectis | Meganuclease variants cleaving a dna target sequence from the mouse rosa26 locus and uses thereof |
JP2011517838A (en) | 2008-04-11 | 2011-06-16 | ユーティーシー パワー コーポレイション | Bipolar plate and fuel cell with manifold sump |
JP5952263B2 (en) | 2010-04-26 | 2016-07-13 | サンガモ バイオサイエンシーズ, インコーポレイテッド | Genome editing of the ROSA locus using zinc finger nuclease |
CN103025344B (en) | 2010-05-17 | 2016-06-29 | 桑格摩生物科学股份有限公司 | Novel DNA-associated proteins and application thereof |
SG10201811736QA (en) | 2011-09-21 | 2019-02-27 | Sangamo Therapeutics Inc | Methods and compositions for regulation of transgene expression |
WO2013063315A2 (en) | 2011-10-27 | 2013-05-02 | Sangamo Biosciences, Inc. | Methods and compositions for modification of the hprt locus |
US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
WO2013141680A1 (en) | 2012-03-20 | 2013-09-26 | Vilnius University | RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX |
SG11201406547YA (en) | 2012-04-25 | 2014-11-27 | Regeneron Pharma | Nuclease-mediated targeting with large targeting vectors |
ES2960803T3 (en) | 2012-05-25 | 2024-03-06 | Univ California | Methods and compositions for RNA-directed modification of target DNA and for modulation of RNA-directed transcription |
WO2014033644A2 (en) | 2012-08-28 | 2014-03-06 | Novartis Ag | Methods of nuclease-based genetic engineering |
ES2926021T3 (en) | 2012-10-23 | 2022-10-21 | Toolgen Inc | Composition for cleaving a target DNA comprising a target DNA-specific guide RNA and Cas protein-encoding nucleic acid or Cas protein, and use thereof |
EP3617309A3 (en) | 2012-12-06 | 2020-05-06 | Sigma Aldrich Co. LLC | Crispr-based genome modification and regulation |
SG10201912328UA (en) | 2012-12-12 | 2020-02-27 | Broad Inst Inc | Delivery, Engineering and Optimization of Systems, Methods and Compositions for Sequence Manipulation and Therapeutic Applications |
US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
IL308158A (en) | 2012-12-17 | 2023-12-01 | Harvard College | Rna-guided human genome engineering |
MX2015010841A (en) | 2013-02-20 | 2016-05-09 | Regeneron Pharma | Genetic modification of rats. |
EP2922393B2 (en) | 2013-02-27 | 2022-12-28 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) | Gene editing in the oocyte by cas9 nucleases |
US9234213B2 (en) | 2013-03-15 | 2016-01-12 | System Biosciences, Llc | Compositions and methods directed to CRISPR/Cas genomic engineering systems |
US10119133B2 (en) * | 2013-03-15 | 2018-11-06 | The General Hospital Corporation | Using truncated guide RNAs (tru-gRNAs) to increase specificity for RNA-guided genome editing |
EP2981617B1 (en) | 2013-04-04 | 2023-07-05 | President and Fellows of Harvard College | Therapeutic uses of genome editing with crispr/cas systems |
DK2986729T3 (en) | 2013-04-16 | 2018-10-29 | Regeneron Pharma | TARGETED MODIFICATION OF ROOT THROUGH |
US20140349405A1 (en) | 2013-05-22 | 2014-11-27 | Wisconsin Alumni Research Foundation | Rna-directed dna cleavage and gene editing by cas9 enzyme from neisseria meningitidis |
AU2014281028B2 (en) | 2013-06-17 | 2020-09-10 | Massachusetts Institute Of Technology | Delivery and use of the CRISPR-Cas systems, vectors and compositions for hepatic targeting and therapy |
CA2915845A1 (en) | 2013-06-17 | 2014-12-24 | The Broad Institute, Inc. | Delivery, engineering and optimization of systems, methods and compositions for targeting and modeling diseases and disorders of post mitotic cells |
EP3011035B1 (en) | 2013-06-17 | 2020-05-13 | The Broad Institute, Inc. | Assay for quantitative evaluation of target site cleavage by one or more crispr-cas guide sequences |
CN106222197A (en) | 2013-07-16 | 2016-12-14 | 中国科学院上海生命科学研究院 | Plant Genome pointed decoration method |
KR102407354B1 (en) | 2013-09-23 | 2022-06-10 | 리제너론 파마슈티칼스 인코포레이티드 | Non-human animals having a humanized signal-regulatory protein gene |
US20160237455A1 (en) | 2013-09-27 | 2016-08-18 | Editas Medicine, Inc. | Crispr-related methods and compositions |
CN105899665B (en) | 2013-10-17 | 2019-10-22 | 桑格摩生物科学股份有限公司 | The delivering method and composition being transformed for nuclease-mediated genome project |
RU2685914C1 (en) | 2013-12-11 | 2019-04-23 | Регенерон Фармасьютикалс, Инк. | Methods and compositions for genome targeted modification |
US9840699B2 (en) | 2013-12-12 | 2017-12-12 | President And Fellows Of Harvard College | Methods for nucleic acid editing |
CA2939942A1 (en) | 2014-02-24 | 2015-08-27 | Sangamo Biosciences, Inc. | Methods and compositions for nuclease-mediated targeted integration |
ES2898460T3 (en) | 2014-02-27 | 2022-03-07 | Monsanto Technology Llc | Compositions and methods for site-directed genomic modification |
WO2015179540A1 (en) | 2014-05-20 | 2015-11-26 | Regents Of The University Of Minnesota | Method for editing a genetic sequence |
WO2015191693A2 (en) | 2014-06-10 | 2015-12-17 | Massachusetts Institute Of Technology | Method for gene editing |
JP6930834B2 (en) | 2014-06-16 | 2021-09-01 | ザ・ジョンズ・ホプキンス・ユニバーシティー | Compositions and Methods for Expression of CRISPR Guide RNA Using the H1 Promoter |
EP3155099B1 (en) | 2014-06-23 | 2018-03-21 | Regeneron Pharmaceuticals, Inc. | Nuclease-mediated dna assembly |
US20150376586A1 (en) | 2014-06-25 | 2015-12-31 | Caribou Biosciences, Inc. | RNA Modification to Engineer Cas9 Activity |
ES2901074T3 (en) | 2014-06-26 | 2022-03-21 | Regeneron Pharma | Methods and compositions for targeted genetic modifications and methods of use |
EP2966170A1 (en) | 2014-07-10 | 2016-01-13 | Heinrich-Pette-Institut Leibniz-Institut für experimentelle Virologie-Stiftung bürgerlichen Rechts - | HBV inactivation |
EP3169309B1 (en) | 2014-07-16 | 2023-05-10 | Novartis AG | Method of encapsulating a nucleic acid in a lipid nanoparticle host |
US20160081314A1 (en) | 2014-09-19 | 2016-03-24 | Regeneron Pharmaceuticals, Inc. | Chimeric Antigen Receptors |
WO2016049024A2 (en) | 2014-09-24 | 2016-03-31 | The Broad Institute Inc. | Delivery, use and therapeutic applications of the crispr-cas systems and compositions for modeling competition of multiple cancer mutations in vivo |
TWI716367B (en) | 2014-10-31 | 2021-01-21 | 麻省理工學院 | Massively parallel combinatorial genetics for crispr |
WO2016073990A2 (en) | 2014-11-07 | 2016-05-12 | Editas Medicine, Inc. | Methods for improving crispr/cas-mediated genome-editing |
NZ731962A (en) | 2014-11-21 | 2022-07-01 | Regeneron Pharma | Methods and compositions for targeted genetic modification using paired guide rnas |
WO2016089866A1 (en) | 2014-12-01 | 2016-06-09 | President And Fellows Of Harvard College | Rna-guided systems for in vivo gene editing |
MX2017008190A (en) * | 2014-12-19 | 2018-03-23 | Regeneron Pharma | Methods and compositions for targeted genetic modification through single-step multiple targeting. |
WO2016106236A1 (en) | 2014-12-23 | 2016-06-30 | The Broad Institute Inc. | Rna-targeting system |
EP3242938B1 (en) | 2015-01-09 | 2020-01-08 | Bio-Rad Laboratories, Inc. | Detection of genome editing |
CN107429263A (en) | 2015-01-15 | 2017-12-01 | 斯坦福大学托管董事会 | The method of controlling gene group editor |
WO2016123514A1 (en) | 2015-01-29 | 2016-08-04 | University Of Massachusetts | Nanoparticle-protein complex for intracellular protein delivery |
WO2016132122A1 (en) | 2015-02-17 | 2016-08-25 | University Of Edinburgh | Assay construct |
US20180245073A1 (en) | 2015-02-23 | 2018-08-30 | Voyager Therapeutics, Inc. | Regulatable expression using adeno-associated virus (aav) |
WO2016176191A1 (en) | 2015-04-27 | 2016-11-03 | The Trustees Of The University Of Pennsylvania | Dual aav vector system for crispr/cas9 mediated correction of human disease |
US10179918B2 (en) | 2015-05-07 | 2019-01-15 | Sangamo Therapeutics, Inc. | Methods and compositions for increasing transgene activity |
US9790490B2 (en) | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
EP3159407A1 (en) | 2015-10-23 | 2017-04-26 | Silence Therapeutics (London) Ltd | Guide rnas, methods and uses |
CN106893739A (en) * | 2015-11-17 | 2017-06-27 | 香港中文大学 | For the new method and system of target gene operation |
JP6997708B2 (en) | 2015-11-20 | 2022-02-04 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | Non-human animal carrying the humanized LYMPHOCYTE-ACTIVATION GENE 3 gene |
US10240145B2 (en) * | 2015-11-25 | 2019-03-26 | The Board Of Trustees Of The Leland Stanford Junior University | CRISPR/Cas-mediated genome editing to treat EGFR-mutant lung cancer |
EP3402884A1 (en) | 2016-01-15 | 2018-11-21 | Astrazeneca AB | Gene modification assays |
CN105647968B (en) | 2016-02-02 | 2019-07-23 | 浙江大学 | A kind of CRISPR/Cas9 working efficiency fast testing system and its application |
KR102617874B1 (en) | 2016-03-30 | 2023-12-22 | 인텔리아 테라퓨틱스, 인크. | Lipid nanoparticle formulations for CRISPR/CAS components |
CN116254231A (en) | 2016-04-25 | 2023-06-13 | 巴塞尔大学 | Allele editing and uses thereof |
KR20170123581A (en) | 2016-04-28 | 2017-11-08 | 연세대학교 산학협력단 | Method for in vivo high-throughput evaluating of RNA-guided nuclease activity |
IL267024B2 (en) | 2016-12-08 | 2023-12-01 | Intellia Therapeutics Inc | MODIFIED GUIDE RNAs FOR GENOMIC EDITING |
US20180185516A1 (en) | 2016-12-09 | 2018-07-05 | Sangamo Therapeutics, Inc. | Delivery of target specific nucleases |
CN110582302A (en) | 2016-12-14 | 2019-12-17 | 利甘达尔股份有限公司 | Compositions and methods for nucleic acid and/or protein payload delivery |
US11060153B2 (en) | 2016-12-22 | 2021-07-13 | Yale University | Fluorescence amplification method for forward genetic discovery of factors in human mRNA degradation |
EP3625346A1 (en) | 2017-05-16 | 2020-03-25 | Cairn Biosciences, Inc. | Multiplex assay |
US11332749B2 (en) | 2017-07-13 | 2022-05-17 | Regents Of The University Of Minnesota | Real-time reporter systems for monitoring base editing |
CA3065579A1 (en) | 2017-07-31 | 2019-02-07 | Regeneron Pharmaceuticals, Inc. | Assessment of crispr/cas-induced recombination with an exogenous donor nucleic acid in vivo |
SG11201912236YA (en) | 2017-07-31 | 2020-01-30 | Regeneron Pharma | Crispr reporter non-human animals and uses thereof |
WO2019089761A1 (en) | 2017-10-31 | 2019-05-09 | The Broad Institute, Inc. | Crispr protein inhibitors |
WO2019089803A1 (en) | 2017-10-31 | 2019-05-09 | The Broad Institute, Inc. | Methods and compositions for studying cell evolution |
US20210123072A1 (en) | 2018-04-20 | 2021-04-29 | Dana-Farber Cancer Institute, Inc. | Multifunctional nucleic acid reporter constructs |
US11690921B2 (en) | 2018-05-18 | 2023-07-04 | Sangamo Therapeutics, Inc. | Delivery of target specific nucleases |
EP3802828A4 (en) | 2018-06-08 | 2022-10-26 | Intellia Therapeutics, Inc. | Modified guide rnas for gene editing |
WO2019246203A1 (en) | 2018-06-19 | 2019-12-26 | The Board Of Regents Of The University Of Texas System | Lipid nanoparticle compositions for delivery of mrna and long nucleic acids |
WO2020047531A1 (en) | 2018-08-31 | 2020-03-05 | The Children's Hospital Of Philadelphia | Scalable tagging of endogenous genes by homology-independent intron targeting |
WO2020146732A1 (en) | 2019-01-11 | 2020-07-16 | North Carolina State University | Compositions and methods related to reporter systems and large animal models for evaluating gene editing technology |
-
2018
- 2018-07-31 SG SG11201912236YA patent/SG11201912236YA/en unknown
- 2018-07-31 CN CN201880047561.3A patent/CN111182790A/en active Pending
- 2018-07-31 US US16/050,806 patent/US11021719B2/en active Active
- 2018-07-31 JP JP2020504690A patent/JP2020533957A/en active Pending
- 2018-07-31 CA CA3068072A patent/CA3068072A1/en active Pending
- 2018-07-31 EP EP18766066.7A patent/EP3585160A2/en not_active Withdrawn
- 2018-07-31 WO PCT/US2018/044606 patent/WO2019028023A2/en unknown
- 2018-07-31 KR KR1020207001975A patent/KR20200033259A/en not_active Application Discontinuation
- 2018-07-31 BR BR112020001996-8A patent/BR112020001996A2/en unknown
- 2018-07-31 MX MX2020001177A patent/MX2020001177A/en unknown
- 2018-07-31 AU AU2018309708A patent/AU2018309708A1/en not_active Abandoned
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2020
- 2020-01-29 IL IL272333A patent/IL272333A/en unknown
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2021
- 2021-04-30 US US17/245,405 patent/US20210261985A1/en not_active Abandoned
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US11021719B2 (en) | 2021-06-01 |
RU2020105065A3 (en) | 2021-11-10 |
MX2020001177A (en) | 2020-09-25 |
CN111182790A (en) | 2020-05-19 |
IL272333A (en) | 2020-03-31 |
US20190032092A1 (en) | 2019-01-31 |
CA3068072A1 (en) | 2019-02-07 |
WO2019028023A3 (en) | 2019-04-04 |
AU2018309708A1 (en) | 2020-02-06 |
US20210261985A1 (en) | 2021-08-26 |
EP3585160A2 (en) | 2020-01-01 |
JP2020533957A (en) | 2020-11-26 |
KR20200033259A (en) | 2020-03-27 |
WO2019028023A4 (en) | 2019-06-13 |
BR112020001996A2 (en) | 2020-08-18 |
RU2020105065A (en) | 2021-09-02 |
WO2019028023A2 (en) | 2019-02-07 |
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