SG11201903797SA - An array of needle manipulators for biological cell injection - Google Patents

An array of needle manipulators for biological cell injection

Info

Publication number
SG11201903797SA
SG11201903797SA SG11201903797SA SG11201903797SA SG11201903797SA SG 11201903797S A SG11201903797S A SG 11201903797SA SG 11201903797S A SG11201903797S A SG 11201903797SA SG 11201903797S A SG11201903797S A SG 11201903797SA SG 11201903797S A SG11201903797S A SG 11201903797SA
Authority
SG
Singapore
Prior art keywords
international
needle
cell
array
micro
Prior art date
Application number
SG11201903797SA
Inventor
Arunava Steven Banerjee
Original Assignee
Mekonos Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2016904437A external-priority patent/AU2016904437A0/en
Application filed by Mekonos Ltd filed Critical Mekonos Ltd
Publication of SG11201903797SA publication Critical patent/SG11201903797SA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B25—HAND TOOLS; PORTABLE POWER-DRIVEN TOOLS; MANIPULATORS
    • B25J—MANIPULATORS; CHAMBERS PROVIDED WITH MANIPULATION DEVICES
    • B25J7/00—Micromanipulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/50—Means for positioning or orientating the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Robotics (AREA)
  • Fluid Mechanics (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Mechanical Engineering (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Manipulator (AREA)
  • Computer Hardware Design (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Micromachines (AREA)
  • Infusion, Injection, And Reservoir Apparatuses (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 111111111111111111111010111 0111110101111111111111111111111010111111111011110111111 Organization International Bureau (10) International Publication Number (43) International Publication Date .....0\"\" WO 2018/080324 Al 03 May 2018 (03.05.2018) W I PO I PCT (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every C12N 15/89 (2006.01) C12M 3/00 (2006.01) kind of national protection available): AE, AG, AL, AM, (21) International Application Number: AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, PCT/NZ2017/050140 CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, (22) International Filing Date: HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, 31 October 2017 (31.10.2017) KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, (25) Filing Language: English OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (26) Publication Language: English SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, (30) Priority Data: TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. 2016904437 31 October 2016 (31.10.2016) AU (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (71) Applicant: MEKONOS LIMITED [NZ/NZ]; 101 Pack- GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, enham St, W., 1010 Auckland (NZ). UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, (72) Inventor: BANERJEE, Arunava Steven; Skydeck, 2150 TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, Shattuck Avenue, Berkeley, 94704 (US). EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, (74) Agent: AJ PARK; State Insurance Tower, 1 Willis Street, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, PO Box 949, Wellington 6140 (NZ). KM, ML, MR, NE, SN, TD, TG). = (54) Title: AN ARRAY OF NEEDLE MANIPULATORS FOR BIOLOGICAL CELL INJECTION = gb , _ 643 = - = 8.h • gb2 8113 = _ - _ — , = = = = \ \ _ = 6 c Il 71' ei M 0 (57) : A device is provided, comprising a cell GC to hold a cell. The device can further comprise a manipulator ---- communication with a respective micro-chamber, wherein GC needle is mounted to the stage, and the actuator is operable to apply 1-1 © a cell in the respective micro-chamber. ei r ' .:..: 4 '''V')` ::.:.':. . ... 58 Figure trap comprising array each , . 8a1 Q.a 1 a plurality comprising a plurality of manipulators, each manipulator in spatial manipulator comprises force to 5. , 4 , e . e A 6a 883 of micro-chambers, each micro-chamber configured a needle, a stage, and an actuator, wherein the the stage in a direction to move the needle to penetrate C [Continued on next page] WO 2018/080324 Al MIDEDIMOM0101011EFIENIRM01111011010HEMOVOIS Published: — with international search report (Art. 21(3)) — before the expiration of the time limit for amending the claims and to be republished in the event amendments (Rule 48.2(h)) of receipt of
SG11201903797SA 2016-10-31 2017-10-31 An array of needle manipulators for biological cell injection SG11201903797SA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2016904437A AU2016904437A0 (en) 2016-10-31 An Array of Needle Manipulators for Biological Cell Injection
PCT/NZ2017/050140 WO2018080324A1 (en) 2016-10-31 2017-10-31 An array of needle manipulators for biological cell injection

Publications (1)

Publication Number Publication Date
SG11201903797SA true SG11201903797SA (en) 2019-05-30

Family

ID=62025227

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201903797SA SG11201903797SA (en) 2016-10-31 2017-10-31 An array of needle manipulators for biological cell injection

Country Status (8)

Country Link
US (2) US11680277B2 (en)
EP (1) EP3532622A4 (en)
KR (2) KR102470238B1 (en)
CN (1) CN110418844B (en)
AU (1) AU2017349494B2 (en)
CA (1) CA3041777A1 (en)
SG (1) SG11201903797SA (en)
WO (1) WO2018080324A1 (en)

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US11680277B2 (en) 2016-10-31 2023-06-20 Mekonos Limited Array of needle manipulators for biological cell injection
CN111979110B (en) * 2020-07-08 2022-02-15 北京理工大学 Micro-target screening device based on multi-needle array vibration excitation fluid

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Also Published As

Publication number Publication date
EP3532622A4 (en) 2020-06-03
KR102649456B1 (en) 2024-03-19
AU2017349494B2 (en) 2024-02-01
CN110418844A (en) 2019-11-05
AU2017349494A1 (en) 2019-06-06
EP3532622A1 (en) 2019-09-04
KR20220162811A (en) 2022-12-08
KR102470238B1 (en) 2022-11-23
US20190300907A1 (en) 2019-10-03
US11680277B2 (en) 2023-06-20
WO2018080324A9 (en) 2019-04-18
CA3041777A1 (en) 2018-05-03
US20240018549A1 (en) 2024-01-18
CN110418844B (en) 2024-04-09
WO2018080324A1 (en) 2018-05-03
KR20190070987A (en) 2019-06-21

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