SG11201808302VA - Methods and systems for determining a drop delay of a flow stream in a flow cytometer - Google Patents

Methods and systems for determining a drop delay of a flow stream in a flow cytometer

Info

Publication number
SG11201808302VA
SG11201808302VA SG11201808302VA SG11201808302VA SG11201808302VA SG 11201808302V A SG11201808302V A SG 11201808302VA SG 11201808302V A SG11201808302V A SG 11201808302VA SG 11201808302V A SG11201808302V A SG 11201808302VA SG 11201808302V A SG11201808302V A SG 11201808302VA
Authority
SG
Singapore
Prior art keywords
flow stream
international
methods
drop delay
systems
Prior art date
Application number
SG11201808302VA
Inventor
Pierce Norton
Original Assignee
Becton Dickinson Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson Co filed Critical Becton Dickinson Co
Publication of SG11201808302VA publication Critical patent/SG11201808302VA/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1425—Optical investigation techniques, e.g. flow cytometry using an analyser being characterised by its control arrangement
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/20—Analysis of motion
    • G06T7/246—Analysis of motion using feature-based methods, e.g. the tracking of corners or segments
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/626—Detection means characterised by use of a special device being a flow cytometer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G01N2015/1406—Control of droplet point

Landscapes

  • Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Immunology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Theoretical Computer Science (AREA)
  • Signal Processing (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Information Retrieval, Db Structures And Fs Structures Therefor (AREA)

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1#111111101110101011111 3E1 0 011101E11111 111111111111111111 0111111 Organization 0 International Bureau (10) International Publication Number (43) International Publication Date .....0 WO 2018/067210 Al 12 April 2018 (12.04.2018) W I PO I PC T (51) International Patent Classification: (74) Agent: FIELD, Bret, E.; 201 Redwood Shores Parkway, GO1N 15/14 (2006.01) Suite 200, Redwood City, California 94065 (US). (21) International Application Number: (81) Designated States (unless otherwise indicated, for every PCT/US2017/037508 kind of national protection available): AE, AG, AL, AM, (22) International Filing Date: AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, 14 June 2017 (14.06.2017) CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, (25) Filing Language: English HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, (26) Publication Language: English KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, (30) Priority Data: OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, 62/403,553 03 October 2016 (03.10.2016) US SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (71) Applicant: BECTON, DICKINSON AND COMPANY [US/US]; 1 Becton Drive, MC110, Franklin Lakes, New (84) Designated States (unless otherwise indicated, for every Jersey 07417-1880 (US). kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, (72) Inventor: NORTON, Pierce, 0.; 16280 Greenridge Ter- UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, race, Los Gatos, California 95032 (US). TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, Title: METHODS AND SYSTEMS FOR DETERMINING A DROP DELAY OF A FLOW STREAM IN A FLOW CYTOME- (54) = TER = = Step 1 = • = = Step 2 = = = = Step 3A Step 3B = = = _ = Step 4A Step 4B = 1 ,-1 d © ,-1 Step 5 FIG. 1 ei IN (57) : Methods and systems for determining a drop delay of a flow stream in a flow cytometer are provided. Aspects of the ' t:: methods according to certain embodiments include capturing an image of the flow stream to obtain an imaged flow stream, identifying 0 ---- a disturbance at a break off point in the imaged flow stream and calculating the drop delay of the flow stream based on the identified GO ,_ 1 disturbance. Systems for practicing the subject methods having an imaging sensor for capturing one or more images of the flow stream 0 and a processor configured to identify a disturbance at a break off in the imaged flow stream and calculating the drop delay using the N imaged flow stream are also provided. Non-transitory computer readable storage mediums are also described. C [Continued on next page] WO 2018/067210 Al MIDEDIMOMMIDIREEMOTINHOHOMMOVOIS MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3))
SG11201808302VA 2016-10-03 2017-06-14 Methods and systems for determining a drop delay of a flow stream in a flow cytometer SG11201808302VA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662403553P 2016-10-03 2016-10-03
PCT/US2017/037508 WO2018067210A1 (en) 2016-10-03 2017-06-14 Methods and systems for determining a drop delay of a flow stream in a flow cytometer

Publications (1)

Publication Number Publication Date
SG11201808302VA true SG11201808302VA (en) 2018-10-30

Family

ID=61757970

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201808302VA SG11201808302VA (en) 2016-10-03 2017-06-14 Methods and systems for determining a drop delay of a flow stream in a flow cytometer

Country Status (8)

Country Link
US (2) US10732094B2 (en)
EP (1) EP3519797A4 (en)
JP (1) JP6983870B2 (en)
KR (1) KR102416357B1 (en)
CN (1) CN109073530B (en)
AU (1) AU2017340136B2 (en)
SG (1) SG11201808302VA (en)
WO (1) WO2018067210A1 (en)

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Also Published As

Publication number Publication date
KR102416357B1 (en) 2022-07-04
JP2019529883A (en) 2019-10-17
EP3519797A4 (en) 2020-06-17
AU2017340136A1 (en) 2018-10-18
US11656168B2 (en) 2023-05-23
CN109073530B (en) 2022-04-26
WO2018067210A1 (en) 2018-04-12
US20200326271A1 (en) 2020-10-15
US10732094B2 (en) 2020-08-04
KR20190050935A (en) 2019-05-14
US20180095024A1 (en) 2018-04-05
AU2017340136B2 (en) 2022-02-24
EP3519797A1 (en) 2019-08-07
JP6983870B2 (en) 2021-12-17
CN109073530A (en) 2018-12-21

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